| Product Name |
CHO-K1/VPAC1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant VPAC1 Receptor Stable Cell Line |
|
Species |
Human |
Description |
The widespread neuropeptide vasoactive intestinal peptide (VIP) has two receptors VPAC1 and VPAC2. The vasoactive intestinal polypeptide receptor VPAC1 is Gs-coupled GPCRs expressed in the lung, prostate, peripheral blood leukocytes, liver, brain, small intestine colon, heart, spleen, placenta, kidney, thymus, and testis. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for VPAC1 receptor |
Application Examples |
Figure 1. VIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/VPAC1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a VPAC1 receptor agonist, VIP. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of VIP (Mean ± SD, n = 2). The EC50 of VIP on VPAC1 co-expressing with Gα15 in CHO-K1 cells was 11 nM. The S/B of VIP on VPAC1 co-expressing with Gα15 in CHO-K1 cells was 12. |
Related Products
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
|
Document-EXAMPLE: 11765_20100131212933.JPG (JPG)
TECHNICAL MANUAL: 13120_20100714222318.PDF (PDF)