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Cat. No.
Product Name
Size
Price
Quantity
M00342 CHO-K1/VPAC1/Gα15 Stable Cell Line
2 vials
$8500.00
Product Name CHO-K1/VPAC1/Gα15 Stable Cell Line
Species
Human
Documents
CHO-K1/VPAC1/Gα15 Stable Cell LineTECHNICAL MANUAL: M00342_Manual.pdf (pdf)
CHO-K1/VPAC1/Gα15 Stable Cell LineDocument-MSDS: 13242_20100815215655.PDF (PDF)
Figures
CHO-K1/VPAC1/Gα15 Stable Cell Line zoomCHO-K1/VPAC1/Gα15 Stable Cell Line
Description
The widespread neuropeptide vasoactive intestinal peptide (VIP) has two receptors VPAC1 and VPAC2. The vasoactive intestinal polypeptide receptor VPAC1 is Gs-coupled GPCRs expressed in the lung, prostate, peripheral blood leukocytes, liver, brain, small intestine colon, heart, spleen, placenta, kidney, thymus, and testis.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for VPAC1 receptor
Application Examples
Figure 1. VIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/VPAC1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a VPAC1 receptor agonist, VIP. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of VIP (Mean ± SD, n = 2). The EC50 of VIP on VPAC1 co-expressing with Gα15 in CHO-K1 cells was 11 nM. The S/B of VIP on VPAC1 co-expressing with Gα15 in CHO-K1 cells was 12.
Document-COA
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