| Product Name |
CHO-K1/CRF1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Corticotropin Releasing Factor Receptor CRF1 Stable Cell Line |
|
Species |
Human |
Description |
The CRF1 receptor is a Gs-coupled GPCR expressed in the brain and pituitary gland that binds to several neuropeptides, including corticotropin-releasing factor (CRF) and urocortin, and the amphibian peptide sauvagine. CRF plays a predominant role in stress response mediated by the hypothalamic-pituitary-adrenal axis, and alterations in CRF and its receptors CRF1 and CRF2 appear to be linked to depression and anxiety. In comparison to the CRF2 receptor, the CRF1 receptor has received considerable attention as a potential therapeutic target for the treatment of stress-related disorders such as adrenocorticotropin hypersecretion, increased colonic motility and exaggerated fear and anxiety-related behavior. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CRF1 receptor |
Application Examples |
Figure 1. Corticotro-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CRF1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a CRF1 receptor agonist, Corticotro. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Corticotro (Mean ± SD, n = 2). The EC50 of Corticotro on CRF1 co-expressing with Gα15 in CHO-K1 cells was 51 nM. The S/B of Corticotro on CRF1 co-expressing with Gα15 in CHO-K1 cells was 21. |
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