| Product Name |
HEK293/GCGR/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Glucagon Receptor Stable Cell Line |
|
Species |
Human |
Description |
Glucagon regulates blood glucose via control of hepatic glycogenolysis and gluconeogenesis and via regulation of insulin release from the β cell. Pharmacological administration of glucagon increases blood glucose in normal and diabetic subjects, and produces positive inotropic and chronotropic cardiovascular effects, relaxation of smooth muscle in the gastrointestinal tract and stimulation of growth hormone secretion. The actions of glucagon are mediated via a single adenylate cyclase-coupled glucagon receptor that also couples to the phospholipase C-inositol phosphate (PLC-IP) pathway leading to Ca2+ release from intracellular stores. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 50 μg/ml Hygromycin B, 300 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for glucagon receptor |
Application Examples |
Figure 1. Glucagon-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/GCGR/Gα15 and HEK293 cells. The cells were loaded with Calcium-4 prior to stimulation with a GCGR receptor agonist, Glucagon. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Glucagon (Mean ± SD, n = 2). The EC50 of Glucagon on GCGR co-expressing with Gα15 in HEK293 cells was 9.4 nM. The S/B of Glucagon on GCGR co-expressing with Gα15 in HEK293 cells was 18. |
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TECHNICAL MANUAL: 13400_20101018234617.PDF (PDF)
Document-EXAMPLE: 13181_20100812020420.JPG (JPG)