293/5-HT7/CRE-Luc Stable Cell Line

*This product has been discontinued!*

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT7 receptor is a Gs-coupled GPCR which is expressed in the heart and brain. Splice variants of the 5-HT7 receptor have been found in the human brain, 5-HT7(a), 5-HT7(b) and 5-HT7(d). Differing only in the lengths of their carboxy terminal, these variants are all functional receptors. GenScript's cloned human 5-HT7-expressing cell line is generated in the 293/CRE-Luc host.

Cat. No.	M00423
Size	Buy in bulk

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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant HEK293 cells stably overexpress human 5-hydroxytryptamine receptor 7 (HTR7) on the surface and contain high levels of G protein Gαs to couple with the inducible luciferase in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	DMEM, 10% FBS
Freeze Medium-DATA	50% FBS (Cat. No. 10099-141, Life Technologies), 45% DMEM (Cat. No. 10566, Life Technologies) 5% DMSO (Cat. No. D2650, Sigma)

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.