| Product Name |
293/5-HT7/CRE-Luc Stable Cell Line |
Full Name |
Human Recombinant 5-HT7 Serotonin Receptor Stable Cell Line |
|
Species |
Human |
Description |
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT7 receptor is a Gs-coupled GPCR which is expressed in the heart and brain. Splice variants of the 5-HT7 receptor have been found in the human brain, 5-HT7(a), 5-HT7(b) and 5-HT7(d). Differing only in the lengths of their carboxy terminal, these variants are all functional receptors. GenScript's cloned human 5-HT7-expressing cell line is generated in the 293/CRE-Luc host. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for 5-HT7 receptor |
 |
1 |
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology. |
2 |
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days. |
3 |
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package. |
4 |
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50. |
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TECHNICAL MANUAL: 13537_20101216013714.PDF (PDF)
Document-EXAMPLE: 13539_20101216013849.JPG (JPG)