| Product Name |
CHO-K1/MT1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Melatonin MT1 Receptor Stable Cell Line |
|
Species |
Human |
Description |
Melatonin binds to two specific G-protein coupled receptors (GPCR), MT1 (MTNR1A/MEL1A) and MT2 (MTNR1B/MEL1B). MT1 receptors signal via inhibitory G proteins (Gαi and Gαo) leading to adenylate cyclase inhibition and possibly inositol phosphate stimulation in recombinant systems. In certain native tissues (e.g. sheep pars tuberalis, rat cerebral and caudal arteries) melatonin responses are presumably mediated through activation of MT1 receptors. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for MT1 receptor |
Application Examples |
Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an MT1 receptor agonist, Melatonin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Melatonin (Mean ± SD, n = 2). The EC50 of Melatonin on MT1 co-expressing with Gα15 in CHO-K1 cells was 0.36 nM. The S/B of Melatonin on MT1 co-expressing with Gα15 in CHO-K1 cells was 45. |
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