CHO-K1/MRGPRX2 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human mas-related G-protein coupled receptor member X2 (MRGPRX2) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.

Price	$8500.00
Cat. No.	M00425
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human mas-related G-protein coupled receptor member X2 (MRGPRX2) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies).
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS.
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

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Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a MRGPRX2 receptor agonist, cortistatin 14. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (∆RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of cortistatin 14 (Mean ± SEM, n = 3). The EC₅₀ of cortistatin 14 on this cell was 1.53 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.