| Product Name |
CHO-K1/MRGPRX2 Stable Cell Line |
Full Name |
Human Recombinant Mas-related G Protein Coupled Receptor Member X2 Stable Cell Line |
|
Species |
Human |
Description |
MRGX2 is a Gq- and Gi-coupled GPCR that binds to several peptides, including the neuropeptide cortistatin-14 and the proadrenomedullin N-terminal peptide (PAMP). It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Dorsal root ganglia and adrenal chromaffin cells express MRGX2, and binding of these ligands to MRGX2 is thought to play a role in nociception and catecholamine release from the adrenal glands. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for MRGPRX2 receptor |
Application Examples |
Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with an MRGPRX2 receptor agonist, Cortistatin 14. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Cortistatin 14 (Mean ± SD, n = 2). The EC50 of Cortistatin 14 on MRGPRX2 in CHO-K1 cells was 136 nM. The S/B of Cortistatin 14 on MRGPEX2 in CHO-K1 cells was 12. |
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