| Product Name |
CHO-K1/GNRHR/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Gonadotropin-releasing Hormone Receptor Stable Cell Line |
|
Species |
Human |
Description |
The gonadotropin-releasing hormone receptor (GNRHR), also known as the luteinizing hormone releasing hormone receptor (LHRHR), is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Upon activation, the LHRHr stimulates tyrosine phosphatase and elicits the release of LH from the pituitary. Evidence exists showing the presence of LHRH and its receptor in extrapituitary tissues as well as a role in progression of some cancers. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for GNRHR receptor |
Application Examples |
Figure 1. LH-RH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GNRHR/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GNRHR receptor agonist, LH-RH. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of LH-RH (Mean ± SD, n = 2). The EC50 of LH-RH on GNRHR co-expressing with Gα15 in CHO-K1 cells was 41 nM. The S/B of LH-RH on GNRHR co-expressing with Gα15 in CHO-K1 cells was 16. |
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