| Product Name |
CHO-K1/GRM2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Metabotropic Glutamate Receptor 2 Stable Cell Line |
|
Species |
Human |
Description |
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. GRM2 is linked to the inhibition of the cyclic AMP cascade, included in group II metabotropic glutamate receptors. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for GRM2 receptor |
Application Examples |
Figure 1. L-Glutamate-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GRM2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a GRM2 receptor agonist, L-Glutamate. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of L-Glutamate (Mean ± SD, n = 2). The EC50 of L-Glutamate on GRM2 co-expressing with Gα15 in CHO-K1 cells was 34 μM. The S/B of L-Glutamate on GRM2 co-expressing with Gα15 in CHO-K1 cells was 4. |
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