| Product Name |
CHO-K1/NIACR1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Niacin Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
GPR109A is a high affinity receptor for nicotinic acid (niacin) and is a member of the nicotinic acid receptor family of G protein-coupled receptors (the other identified member being GPR109B). GPR109A is a Gi/o protein-coupled receptor with high affinity for nicotinic acid. In GPR109A knockout mice, the effects of niacin on both lipids and flushing are eliminated. Furthermore in arrestin beta 1 knockout mice, niacin's effect on flushing is greatly reduced while the lipid modifying effects are maintained. GPR109A is believed to be an important biomolecular target of niacin which is a widely prescribed drug for the treatment of dyslipidemia and to increase HDL cholesterol but whose therapeutic use is limited by flushing. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for NIACR1 receptor |
Application Examples |
Figure 1. Nicotinic Acid-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NIACR1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an NIACR1 receptor agonist, Nicotinic Acid. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Nicotinic Acid (Mean ± SD, n = 2). The EC50 of Nicotinic Acid on NIACR1 co-expressing with Gα15 in CHO-K1 cells was 49 nM. The S/B of Nicotinic Acid on NIACR1 co-expressing with Gα15 in CHO-K1 cells was 4. |
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