| Product Name |
CHO-K1/FPR1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Formylpeptide Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
The formyl peptide receptors (FPR) are a class of G protein-coupled receptors involved in chemotaxis. FPR1 is a Gi-coupled GPCR. FPR1 is expressed in the vasculature, secretory epithelial cells, neurons and other tissues. FPR is involved in host defense against bacterial infection and in the clearance of damaged cells. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for FPR1 receptor |
Application Examples |
Figure 1. W-peptide-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/FPR1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an FPR1 receptor agonist, w-peptide. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of w-peptide (Mean ± SD, n = 2). The EC50 of w-peptide on FPR1 co-expressing with Gα15 in CHO-K1 cells was 2.0 nM. The S/B of w-peptide on FPR1 co-expressing with Gα15 in CHO-K1 cells was 19. |
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TECHNICAL MANUAL: 13096_20100712065725.PDF (PDF)
Document-EXAMPLE: 13175_20100811050539.JPG (JPG)