Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/CASR Stable Cell Line
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Cat. No.
Name
Size   
Price
Selected
M00434
2 vials
$8,500
Product Name CHO-K1/CASR Stable Cell Line
Full Name
Human Recombinant Calcium Sensor Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: 13057_20100710013403.PDF (PDF)
Document-MSDS: 13226_20100815211820.PDF (PDF)
Figures
CHO-K1/CASR Stable Cell Line zoomCHO-K1/CASR Stable Cell Line
Species
Human
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for CASR receptor
Application Examples
Figure 1. CaCl2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CASR and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a CASR receptor agonist, CaCl2. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (3-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of CaCl2 on CASR in CHO-K1 cells was 0.34 mM. The S/B of CaCl2 on CASR in CHO-K1 cells was 29.
Document-COA
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