Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/CASR Stable Cell Line
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Cat. No.
Name
Size   
Price
Selected
M00434
2 vials
$11,600
Product Name CHO-K1/CASR Stable Cell Line
Full Name
Human Recombinant Calcium Sensor Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: 13057_20100710013403.PDF (PDF)
Document-MSDS: 13226_20100815211820.PDF (PDF)
Figures
CHO-K1/CASR Stable Cell Line zoomCHO-K1/CASR Stable Cell Line
Reference
Species
Human
Description
CASR is a calcium-sensing receptor and plays an important role in regulating PTH secretion. It is expressed in many different tissues, such as parathyroid, pancreatic islet and insulinoma cells, breast, gastrinoma cells, pituitary, keratinocytes and human colon epithelial cells. CASR Inhibits PTH secretion and parathyroid cellular proliferation in parathyroid glands or cells, and also inhibits proliferation of colonic crypt cells. Besides, it can stimulate PTHrP secretion in prostate cell line, and stimulate calcitonin secretion from thyroidal C-cells.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for CASR receptor
Application Examples
Figure 1. CaCl2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CASR and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a CASR receptor agonist, CaCl2. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (3-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of CaCl2 on CASR in CHO-K1 cells was 0.34 mM. The S/B of CaCl2 on CASR in CHO-K1 cells was 29.
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