Catalog Products » Other Products » Stable Cell Lines » Human Recombinant Calcium Sensor Receptor Stable Cell Line
Email to GenScript

Cat. No.
Product Name
M00434 CHO-K1/CASR Stable Cell Line
2 vials
Product Name CHO-K1/CASR Stable Cell Line
CHO-K1/CASR Stable Cell LineTECHNICAL MANUAL: 13057_20100710013403.PDF (PDF)
CHO-K1/CASR Stable Cell LineDocument-MSDS: 13226_20100815211820.PDF (PDF)
CHO-K1/CASR Stable Cell Line zoomCHO-K1/CASR Stable Cell Line
CASR is a calcium-sensing receptor and plays an important role in regulating PTH secretion. It is expressed in many different tissues, such as parathyroid, pancreatic islet and insulinoma cells, breast, gastrinoma cells, pituitary, keratinocytes and human colon epithelial cells. CASR Inhibits PTH secretion and parathyroid cellular proliferation in parathyroid glands or cells, and also inhibits proliferation of colonic crypt cells. Besides, it can stimulate PTHrP secretion in prostate cell line, and stimulate calcitonin secretion from thyroidal C-cells.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin
Liquid nitrogen immediately upon delivery
Functional assay for CASR receptor
Application Examples
Figure 1. CaCl2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CASR and CHO-K1 cells. The cells were loaded with Calcium-4 prior to stimulation with a CASR receptor agonist, CaCl2. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (3-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of CaCl2 on CASR in CHO-K1 cells was 0.34 mM. The S/B of CaCl2 on CASR in CHO-K1 cells was 29.
Related Products
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.23/bp, with advanced FREE codon optimization tool and seamless cloning technology.
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.