| Product Name |
CHO-K1/LTB4/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Leukotriene B4 Receptor Stable Cell Line |
|
Species |
Human |
Description |
The dihydroxy-leukotriene, leukotriene B4 (LTB4) is a member of the G protein-coupled receptor (GPCR) family, in a subfamily of GPCRs that includes receptors for chemokines and other chemotactic factors. Recently, a second, lower affinity receptor for LTB4 (BLT2) has also been cloned, with broader ligand specificity for various eicosanoids. LTB4 stimulates neutrophil chemotaxis and secretion but may also affect immunomodulation, contraction of certain smooth muscles via an indirect mechanism and activation of the nuclear transcription factor PPARα (peroxisome proliferation activated receptor alpha). Chemotaxis, the principal effects of LTB4 and related dihydroxy-acids on leukocytes, occurs via activation of BLT1 receptors. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for LTB4 receptor |
Application Examples |
Figure 1. LTB4-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ LTB4/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an LTB4 receptor agonist, LTB4. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of LTB4 (Mean ± SD, n = 2). The EC50 of LTB4 on LTB4 co-expressing with Gα15 in CHO-K1 cells was 1.1 nM. The S/B of LTB4 on LTB4 co-expressing with Gα15 in CHO-K1 cells was 8. |
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