| Product Name |
CHO-K1/DP2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Prostanoid Receptor DP2 Stable Cell Line |
|
Species |
Human |
Description |
Prostaglandin D(2) (PGD(2)) is derived from arachidonic acid and binds with high affinity to the G-protein coupled receptors prostanoid DP(1) and DP(2). Interaction with DP(2) results in cell chemotaxis, eosinophil degranulation, eosinophil shape change, adhesion molecule upregulation and Th2 cytokine production. In allergic rhinitis and allergic asthma PGD(2) is released from mast cells in response to allergen challenge and may trigger symptoms such as sneezing, rhinorrhea, pruritus, mucus hypersecretion and pulmonary inflammation. GenScript's cloned human DP2-expressing cell line co-expressing Gα15 is made in the CHO-K1 host. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham’s F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for DP2 receptor |
Application Examples |
Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a DP2 receptor agonist, PGD2. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC50 of PGD2 on DP2 co-expressing with Gα15 in CHO-K1 cells was 102 nM. The S/B of PGD2 on DP2 co-expressing with Gα15 in CHO-K1 cells was 6. |
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