Catalog Products » Other Products » Stable Cell Lines » GenScript Stable Cell Line: CHO-K1/FFA2/Gα15 Stable Cell Line
Cat. No.
Name
Size   
Price
Selected
M00437
2 vials
$8,500
Product Name CHO-K1/FFA2/Gα15 Stable Cell Line
Full Name
Human Recombinant Free Fatty Acid Receptor 2 Stable Cell Line
Documents
TECHNICAL MANUAL: 12993_20100628004845.PDF (PDF)
Document-MSDS: 13223_20100815211320.PDF (PDF)
Figures
CHO-K1/FFA2/Gα15 Stable Cell Line zoomCHO-K1/FFA2/Gα15 Stable Cell Line
Species
Human
Description
FFA2 (GPR43) is a 330-amino acid G-protein coupled receptor that binds to fatty acid. FFA2 is highly selective expressed in leukocytes, which suggests a role in the recruitment of these cell populations toward sites of bacterial infection.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for FFA2 receptor
Application Examples
Figure Intracellular calcium response from CHO-K1 cells stably expressing human FFA2 receptor and from untransfected control cells. Cells were loaded with Calcium-4 then stimulated with the indicated concentrations of Sodium Acetate. Calcium responses were recorded on a FlexStation plate reader. Data represent the average +/- standard deviation of triplicate determinations.
Document-COA
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