| Product Name |
CHO-K1/GPR68/Gα15 Stable Cell Line |
Full Name |
Human Recombinant G-Protein Coupled Receptor 68 Stable Cell Line |
|
Species |
Human |
Description |
The GPR68 is a proton-sensing receptor involved in pH homeostasis. A study revealed that in osteosarcoma cells and primary human osteoblast precursors which expression of GPR68 exhibit strong PH-dependent inositol phosphate formation. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for GPR68 receptor |
Application Examples |
Figure 1. H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a GPR68 receptor agonist, H+. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (0.4 log dilution) of H+ (Mean ± SD, n = 2). The EC50 of H+ on GPR68 co-expressing with Gα15 in CHO-K1 cells was pH 5.6. The S/B of H+ on GPR68 co-expressing with Gα15 in CHO-K1 cells was 21. |
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