Catalog Products » Other Products » Stable Cell Lines » Human Recombinant G-Protein Coupled Receptor 68 Stable Cell Line
Email to GenScript

Cat. No.
Product Name
M00439 CHO-K1/GPR68/Gα15 Stable Cell Line
2 vials
Product Name CHO-K1/GPR68/Gα15 Stable Cell Line
CHO-K1/GPR68/Gα15 Stable Cell LineTECHNICAL MANUAL: M00439_protocol.pdf (pdf)
CHO-K1/GPR68/Gα15 Stable Cell LineDocument-MSDS: 13222_20100815211225.PDF (PDF)
CHO-K1/GPR68/Gα15 Stable Cell Line zoomCHO-K1/GPR68/Gα15 Stable Cell Line
The GPR68 is a proton-sensing receptor involved in pH homeostasis. A study revealed that in osteosarcoma cells and primary human osteoblast precursors which expression of GPR68 exhibit strong PH-dependent inositol phosphate formation.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418
Liquid nitrogen immediately upon delivery
Functional assay for GPR68 receptor
Application Examples
Figure 1. H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/Gα15. The cells were loaded with Calcium-4 prior to stimulation with a GPR68 receptor agonist, H+. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (0.4 log dilution) of H+ (Mean ± SD, n = 2). The EC50 of H+ on GPR68 co-expressing with Gα15 in CHO-K1 cells was pH 5.6. The S/B of H+ on GPR68 co-expressing with Gα15 in CHO-K1 cells was 21.
Related Products
Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.23/bp, with advanced FREE codon optimization tool and seamless cloning technology.
PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.