| Product Name |
CHO-K1/PAR2/Gα15 Stable Cell Line |
Full Name |
Human Recombinant PAR2 Proteinase-activated Receptor Stable Cell Line |
|
Species |
Human |
Description |
Proteinase-activated receptors (PAR) are a subfamily of G-protein coupled, seven-transmembrane domain receptors, which are cleaved within the aminoterminal exodomain by certain serine proteinases at a specific peptide bond. Trypsin and mast cell tryptase, and more recently, the activated coagulation factors VIIa and Xa, have been identified as serine proteinases able to activate mammalian PAR-2. As already indicated, PAR-2 is believed to be involved in inflammation. This role for PAR-2 implies that elastase and cathepsin G would paradoxically display an anti-inflammatory property by disarming PAR-2. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for PAR2 receptor |
Application Examples |
Figure 1. Trypsin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PAR2/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a PAR2 receptor agonist, Trypsin. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Trypsin (Mean ± SD, n = 2). The EC50 of Trypsin on PAR2 co-expressing with Gα15 in CHO-K1 cells was 4.6 nM. The S/B of Trypsin on PAR2 co-expressing with Gα15 in CHO-K1 cells was 7. |
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TECHNICAL MANUAL: 13142_20100719041200.PDF (PDF)
Document-EXAMPLE: 12944_20100611233608.JPG (JPG)