| Product Name |
CHO-K1/OPRL1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Opiate Receptor-like 1 Stable Cell Line |
|
Species |
Human |
Description |
Following the cloning of the classical opioid receptors (mu, delta and kappa), the opiate receptor like-1 (ORL1) was identified as a G-protein coupled receptor (GPCR) with 65% structure homology to the other members of the opioid family. OPRL1 is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ and may be involved in the regulation of numerous brain activities, particularly instinctive and emotional behaviors. Recently, new study results are consistent with the reported high density of ORL1 receptor mRNA in dorsal raphe nucleus and with inhibitory actions of nociceptin in cells expressing ORL1. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for OPRL1 receptor |
Application Examples |
Figure 1. Orphanin FQ-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRL1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with an OPRL1 receptor agonist, Orphanin FQ. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Orphanin FQ (Mean ± SD, n = 2). The EC50 of Orphanin FQ on OPRL1 co-expressing with Gα15 in CHO-K1 cells was 6.4 nM. The S/B of Orphanin FQ on OPRL1 co-expressing with Gα15 in CHO-K1 cells was 18. |
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