| Product Name |
293T/AT1 Stable Cell Line |
Full Name |
Human Recombinant Angiotensin Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
Angiotensin receptor 1 (AT1 receptor) is a member of angiotensin receptors which are responsible for the signal transduction of the main effecter hormone. Effects mediated by the AT1 receptor include vasoconstriction, aldosterone synthesis and secretion, increased vasopressin secretion, cardiac hypertrophy, augmentation of peripheral noradrenergic activity, vascular smooth muscle cells proliferation, decreased renal blood flow, renal renin inhibition, renal tubular sodium reuptake, modulation of central sympathetic nervous system activity, cardiac contractility, central osmocontrol and extracellular matrix formation. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 100 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for AT1 receptor |
Application Examples |
Figure 1. Angiotensin II-induced concentration-dependent stimulation of intracellular calcium mobilization in 293T/AT1 and 293T cells. The cells were loaded with Calcium-4 prior to stimulation with an AT1 receptor agonist, Angiotensin II. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of Angiotensin II (Mean ± SD, n = 2). The EC50 of Angiotensin II on AT1 in 293T cells was 19 nM. The S/B of Angiotensin II on AT1 in 293T cells was 27. |
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