| Product Name |
CHO-K1/NPY2/Gqi5 Stable Cell Line |
Full Name |
Human Recombinant Neuropeptide Y Receptor Y2 Stable Cell Line |
|
Species |
Human |
Description |
The NPY family consists of three 36-amino acid peptides, neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), which bind to the NPY receptors. At present five distinct NPY receptors, Y1, Y2, Y4, Y5, and y6, have been established by receptor cloning studies and all of them are Gi-coupled GPCRs. Activation of NPY receptors mediate a variety physiological effects including stimulation of food intake, inhibition of anxiety in the CNS, presynaptic inhibition of neurotransmitter release in the CNS and periphery, modulation of circadian rhythm, release of pituitary hormones, modulation of hippocampal activity, pain transmission, vasoconstriction, inhibition of insulin release and modulation of renal function. With regard to endogenous agonists, the receptors Y2 preferentially bind NPY and PYY. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for NPY2 receptor |
Application Examples |
Figure 1. NPY-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NPY2/Gqi5 and CHO-K1/Gqi5 cells. The cells were loaded with Calcium-4 prior to stimulation with a NPY2 receptor agonist, NPY. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of NPY (Mean ± SD, n = 2). The EC50 of NPY on NPY2 co-expressing with Gqi5 in CHO-K1 cells was 5.6 nM. The S/B of NPY on NPY2 co-expressing with Gqi5 in CHO-K1 cells was 5. |
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TECHNICAL MANUAL: 13762_20110613052014.PDF (PDF)
Document-EXAMPLE: 13764_20110614010830.JPG (JPG)