| Product Name |
CHO-K1/CysLT1/Gα15 Stable Cell Line |
Full Name |
Human Recombinant Cysteinyl Leukotriene Receptor 1 Stable Cell Line |
|
Species |
Human |
Description |
CysLT1 (Cysteinyl leukotriene receptor 1) is previous named as LTD4 receptor (leukotriene D4 receptor). It is a receptor for cysteinyl leukotrienes and has highest affinity to leukotriene D4 (LTD4). The receptor mediates contraction and proliferation of smooth muscle, edema, eosinophil migration and damage to the mucus layer in the lung caused by LTD4. A CysLT1 selective antagonist, montelukast, is used clinically in the treatment of asthma.. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4. |
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS, 400 μg/ml G418, 100 μg/ml Hygromycin B |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for CysLT1 receptor |
Application Examples |
Figure 1. LTD4-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CysLT1/Gα15 and CHO-K1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a CysLT1 receptor agonist, LTD4. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of LTD4 (Mean ± SD, n = 2). The EC50 of LTD4 on CysLT1 in CHO-K1 cells was 0.81 nM. The S/B of LTD4 on CysLT1 in CHO-K1/Gα15 cells was 5. |
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TECHNICAL MANUAL: 13328_20100903015128.PDF (PDF)
Document-EXAMPLE: 13331_20100903020232.JPG (JPG)