| Product Name |
RH7777/EDG4 Stable Cell Line |
Full Name |
Human Recombinant EDG4 Lysophospholipid Receptor Stable Cell Line |
|
Species |
Human |
Description |
Lysophosphatidic acid (LPA), a bioactive lipid produced by several cell types including postmitotic neurons and activated platelets, is thought to be involved in various biological processes, including brain development. Three cognate G-protein coupled receptors encoded by lpa(1)/lp(A1)/Edg-2/Gpcr26, lpa(2)/lp(A2)/Edg-4, and lpa(3)/lp(A3)/Edg-7 mediate the cellular effects of LPA. However, many LPA-induced responses, including phospholipase C activation, Ca2+ mobilization, adenylyl cyclase activation, proliferation, JNK activation, AKT activation, and stress fiber formation, were absent or severely reduced in embryonic fibroblasts derived from lpa(1)(-/-) lpa(2)(-/-) mice. The lysophosphatidic acid receptor LPA2 or endothelial differentiation, G-protein coupled receptor 4(EDG-4) is expressed most abundantly in testes and peripheral blood leukocytes. It is reported to be a distinctive functional marker for ovarian carcinoma. |
Freeze Medium |
45% culture medium, 45%FBS, and 10% DMSO |
Culture Medium |
DMEM, 10% FBS, 100 μg/ml G418 |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assays for EDG4 Receptor |
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1 |
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