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M00473
CHO-K1/SST1/Gqi5 Stable Cell Line
2 vials
Product Name CHO-K1/SST1/Gqi5 Stable Cell Line
Full Name
Human Recombinant SST1 Somatostatin Receptor Stable Cell Line
Documents
TECHNICAL MANUAL: 13694_20110421052701.PDF (PDF)
Document-EXAMPLE: 13696_20110421052856.JPG (JPG)
Figures
Reference
Species
Human
Description
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SST1 receptor is a Gi/Go-coupled GPCR which is expressed in pancreatic islets, pituitary, Cerebellum (Purkinje cells), frontal cortex (pyramidal cells), hippocampus (CA1-4 subfields and some granule cells of the dentate gyrus). It inhibits cAMP accumulation and stimulates tyrosine phosphatase activity, it also can antiproliferation.
GenScript's SST1-expressing stable cell line was made in CHO-K1/Gqi5 host cell and optimized for calcium assays.
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Culture Medium
Ham's F12, 10% FBS
Storage
Liquid nitrogen immediately upon delivery
Application
Functional assay for SST1 receptor
Application Examples
Figure 1. Somatostatin-14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST1/Gqi5 and CHO-K1/Gqi5 cells. The cells were loaded with Calcium-4 prior to stimulation with an SST1 receptor agonist, Somatostatin-14. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Somatostatin-14 (Mean ± SD, n = 2). The EC50 of Somatostatin-14 on SST1 co-expressing with Gqi5 in CHO-K1 cells was 465 nM. The S/B of Somatostatin-14 on SST1 co-expressing with Gqi5 in CHO-K1 cells was 7.
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