| Product Name |
CHO-K1/SST1/Gqi5 Stable Cell Line |
Full Name |
Human Recombinant SST1 Somatostatin Receptor Stable Cell Line |
|
Species |
Human |
Description |
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SST1 receptor is a Gi/Go-coupled GPCR which is expressed in pancreatic islets, pituitary, Cerebellum (Purkinje cells), frontal cortex (pyramidal cells), hippocampus (CA1-4 subfields and some granule cells of the dentate gyrus). It inhibits cAMP accumulation and stimulates tyrosine phosphatase activity, it also can antiproliferation.
GenScript's SST1-expressing stable cell line was made in CHO-K1/Gqi5 host cell and optimized for calcium assays.
|
Freeze Medium |
45% culture medium, 45% FBS, 10% DMSO |
Culture Medium |
Ham's F12, 10% FBS |
Storage |
Liquid nitrogen immediately upon delivery |
Application |
Functional assay for SST1 receptor |
Application Examples |
Figure 1. Somatostatin-14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/SST1/Gqi5 and CHO-K1/Gqi5 cells. The cells were loaded with Calcium-4 prior to stimulation with an SST1 receptor agonist, Somatostatin-14. The intracellular calcium change was measured by FlexStation. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (10-fold dilution) of Somatostatin-14 (Mean ± SD, n = 2). The EC50 of Somatostatin-14 on SST1 co-expressing with Gqi5 in CHO-K1 cells was 465 nM. The S/B of Somatostatin-14 on SST1 co-expressing with Gqi5 in CHO-K1 cells was 7. |
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TECHNICAL MANUAL: 13694_20110421052701.PDF (PDF)
Document-EXAMPLE: 13696_20110421052856.JPG (JPG)