CHO-K1/CTLA4 Stable Cell Line

CTLA4 or CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152 (cluster of differentiation 152), is a protein receptor that functions as an immune checkpoint and downregulates immune responses. CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. The CTLA-4 protein is encoded by the Ctla4 gene in mice and the CTLA4 gene in humans.

Price	$8500.00
Cat. No.	M00530
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human cytotoxic T-lymphocyte associated protein 4 (CTLA4) on the surface. The surface expression of CTLA4 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against CTLA4 or to measure binding affinity between CTLA4 and anti-CTLA4 antibodies.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies).
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies).
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

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Figure 1. FACS analysis of cell surface expression of human CTLA4 on CHO-K1/CTLA4 tailless cells. The CHO-K1/CTLA4 tailless cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CTLA4 antibody (Cat. No. 349906, Biolegend). »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.