CHO-K1/B7-H3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human CD276 (B7-H3) on the surface. The surface expression of B7-H3 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against B7-H3 or to measure binding affinity between B7-H3 and anti-B7-H3 antibodies.

Price	$8500.00
Cat. No.	M00536
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human CD276 (B7-H3) on the surface. The surface expression of B7-H3 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against B7-H3 or to measure binding affinity between B7-H3 and anti-B7-H3 antibodies.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 6 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies).
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

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Figure 1. FACS analysis of cell surface expression of human B7-H3 on CHO-K1/B7-H3 cells. The CHO-K1/B7-H3 cells (blue) and the negative control CHO-K1 cells (red) were probed using anti-human B7-H3 mouse IgG (Cat. No. MAB1027, R&D systems) and FITC-conjugated goat anti-mouse IgG (Cat. No. sc-2010, Santa Cruz). »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.