CHO-K1/OX40 Stable Cell Line

OX40 (CD134) is a member of the TNFR/TNF superfamily. Costimulatory signals from OX40 to a conventional T cell promotes division and survival, augmenting the clonal expansion of effector and memory populations as they are being generated to antigens. OX40 additionally suppresses the differentiation and activity of Treg, further amplifying this process.

Price	$8500.00
Cat. No.	M00561
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human OX40 on the surface. The surface expression of OX40 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against OX40 or to measure binding affinity between OX40 and anti-OX40 antibodies.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies).
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

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Figure 1. FACS analysis of cell surface expression of human OX40 on CHO-K1/OX40 cells. The CHO-K1/OX40 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human OX40 antibody (Cat. No. 350004, Biolegend). »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.