CHO-K1/BDCA2 Stable Cell Line

BDCA-2 is a novel type II C-type lectin, which shows 50.7% sequence identity at the amino acid level to its putative murine ortholog, the murine dendritic cell–associated C-type lectin 2. In addition to its antigen capturing function, BDCA-2 can mediate potent inhibition of induction of IFN-α/β expression in PDCs. Production of IFN-α/β in response to several different types of viruses, bacteria, CpG-DNA, dsRNA, and SLE serum is by far the most prominent feature of PDCs.

Price	$8500.00
Cat. No.	M00595
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human C-type lectin domain family 4 member C (CLEC4C, BDCA2) on the surface. The surface expression of BDCA2 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against BDCA2 or to measure binding affinity between BDCA2 and anti-BDCA2 antibodies.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies).
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

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Figure 1. FACS analysis of cell surface expression of human BDCA2 on CHO-K1/BDCA2 cells. The CHO-K1/BDCA2 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD303 antibody (Cat. No. 130-090-511, Miltenyi Biotec). »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.