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Custom Polyclonal Antibody Services (Mouse)

polyclonal antibody services quotation

Peptide Supplied Polyclonal Antibody Package (Mouse) (Cat. No.: SC1046)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Five Balb/c mice, pre-immune bleed, four immunizations/mouse
  • ELISA test
  • 50-100 µl of pre-immune serum
  • ELISA results
  • 2.5 ml of antisera from all mice
  • 5 mg of unconjugated peptide, MS and HPLC report
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization and ELISA: 10 weeks
  • Shipping: 1-2 weeks
  • Total 16 weeks

Protein A-Purified Peptide Supplied Polyclonal Antibody Package (Mouse) (Cat. No.: SC1049)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Five Balb/C mice, pre-immune, four immunizations/mouse
  • Antibody purification by protein A column
  • ELISA test
  • 50-100 µl of pre-immune serum
  • ELISA results
  • 2.5-12.5 mg purified antibody from all antisera
  • 5 mg of unconjugated peptide, MS and HPLC report
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1 week
  • Shipping: 1-2 weeks
  • Total 17 weeks

Standard Protocol (Five animals)

Steps Time from
Project Initiation
Time from Immunization Activity Description
Peptide synthesis
≈2 weeks
-
-
Peptide conjugation to carrier
≈3 weeks
-
-
Control serum collection
≈4 weeks
Day 0
Pre-bleed (≈0.1 ml serum)
Primary immunization
≈4 weeks
Day 1
0.1 mg per mouse peptide-KLH conjugate, CFA, i.p.
1st boost
≈6 weeks
Day 14
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
Test bleed and ELISA
≈8 weeks
Day 24
0.01-0.02 ml serum per mouse. ELISA and response evaluation.
2nd boost
≈9 weeks
Day 35
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
Test bleed and ELISA
≈11 weeks
Day 45
0.01-0.02 ml serum per mouse. ELISA and response evaluation.
3rd boost
≈12 weeks
Day 56
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
Production bleed
≈14 weeks
Day 66
0.5 ml serum per mouse
ELISA and shipping
≈15 weeks
Day 73
Extend or terminate

Note:

If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.

Quotations and Ordering:

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