Custom Polyclonal Antibody Services (Mouse) | | Peptide Supplied Polyclonal Antibody Package (Mouse) (Cat. No.: SC1046) Includes: | Delivery: | Time Frame: | - Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/c mice, pre-immune bleed, four immunizations/mouse
- ELISA test
| - 50-100 µl of pre-immune serum
- ELISA results
- 2.5 ml of antisera from all mice
- 5 mg of unconjugated peptide, MS and HPLC report
| - Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Shipping: 1-2 weeks
- Total 16 weeks
| Protein A-Purified Peptide Supplied Polyclonal Antibody Package (Mouse) (Cat. No.: SC1049) Includes: | Delivery: | Time Frame: | - Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/C mice, pre-immune, four immunizations/mouse
- Antibody purification by protein A column
- ELISA test
| - 50-100 µl of pre-immune serum
- ELISA results
- 2.5-12.5 mg purified antibody from all antisera
- 5 mg of unconjugated peptide, MS and HPLC report
| - Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Shipping: 1-2 weeks
- Total 17 weeks
| Standard Protocol (Five animals) | Steps | Time from
Project Initiation | Time from Immunization | Activity Description | | Peptide synthesis | ≈2 weeks | | - | | Peptide conjugation to carrier | ≈3 weeks | | - | | Control serum collection | ≈4 weeks | Day 0 | Pre-bleed (≈0.1 ml serum) | | Primary immunization | ≈4 weeks | Day 1 | 0.1 mg per mouse peptide-KLH conjugate, CFA, i.p. | | 1st boost | ≈6 weeks | Day 14 | 0.1 mg per mouse peptide-KLH conjugate, IFA, i.p. | | Test bleed and ELISA | ≈8 weeks | Day 24 | 0.01-0.02 ml serum per mouse. ELISA and response evaluation. | | 2nd boost | ≈9 weeks | Day 35 | 0.1 mg per mouse peptide-KLH conjugate, IFA, i.p. | | Test bleed and ELISA | ≈11 weeks | Day 45 | 0.01-0.02 ml serum per mouse. ELISA and response evaluation. | | 3rd boost | ≈12 weeks | Day 56 | 0.1 mg per mouse peptide-KLH conjugate, IFA, i.p. | | Production bleed | ≈14 weeks | Day 66 | 0.5 ml serum per mouse | | ELISA and shipping | ≈15 weeks | Day 73 | Extend or terminate | Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth. Quotations and Ordering:   For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
 | Email: antibody@genscript.com |  | Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |  | Fax: 1-732-210-0262 1-732-885-5878 | |
