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Custom Polyclonal Antibody Services (Mouse)

Service

Cat. No.
Service Items Antigen Purification
SC1047
Basic Polyclonal Antibody Package (Mouse)
No*
No
SC1250
Basic Protein A-Purified Polyclonal Antibody Package (Mouse)
No*
Protein A
SC1046
Complete Peptide Polyclonal Antibody Package (Mouse)
Yes**
No
SC1049
Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)
Yes**
Protein A
*: Antigens supplied by clients. **: Peptide antigens designed, synthesized, and conjugated by GenScript

Standard Protocol (Five animals)

Steps Time from
Project Initiation
Time from Immunization Activity Description
Peptide synthesis
≈2 weeks
-
-
Peptide conjugation to carrier
≈3 weeks
-
-
Control serum collection
≈4 weeks
Day 0
Pre-bleed (≈0.1 ml serum)
Primary immunization
≈4 weeks
Day 1
0.1 mg per mouse peptide-KLH conjugate, CFA, i.p.
1st boost
≈6 weeks
Day 14
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
Test bleed and ELISA
≈8 weeks
Day 24
0.01-0.02 ml serum per mouse. ELISA and response evaluation.
2nd boost
≈9 weeks
Day 35
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
Test bleed and ELISA
≈11 weeks
Day 45
0.01-0.02 ml serum per mouse. ELISA and response evaluation.
3rd boost
≈12 weeks
Day 56
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
Production bleed
≈14 weeks
Day 66
0.5 ml serum per mouse
Lyophilization
≈15 weeks
Day 73
-
ELISA and shipping
≈16 weeks
Day 80
Extend or terminate

Note:

All antisera or purified antibodies are provided as lyophilized form except special requirements. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.

Quotation and Ordering:
  To request a quotation, please download and complete our Quick Quotation Request.
   To order a pAb, please download & complete our Polyclonal Antibody Order Form.
  To submit an antigen, mail it together with a completed hard copy of Antigen Submission
     Form to Antibody Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.

Please email or fax all quotation requests or completed order forms (including the Antigen Submission Forms) to GenScript.

  If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our Secure Web Server.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Email: antibody@genscript.com
Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Fax: 1-732-210-0262 1-732-885-5878