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Baculovirus Expression System

Baculovirus protein expression service

Baculovirus Expression System

The Baculovirus Expression Vector System (BEVS) is universally recognized as a powerful, versatile means of producing large quantities of recombinant proteins. Its ease of use, high expression level, and ability to incorporate post-translational modifications make it a valuable part of any protein expression arsenal. The GenScript baculovirus expression service package includes both virus production and flexible-scale protein production. GenScript has pioneered methods for the secretion of recombinant proteins from baculovirus-infected insect cells. Our services are suitable for academic institutions and both the pharmaceutical and biotechnology industries. Our current capacity allows to produce proteins at large quantities, up to 500 liters per project.

GenScript protein expression specialists work closely with clients to clone and express full-length recombinant proteins from full-length cDNAs, EST sequence information, and genomic sequences, providing service and expertise at all stages of protein expression and production.

In addition, as the largest gene synthesis supplier in US, GenScript can provide you with free advanced codon optimization specific to Sf9, Sf21, Hi-5 and other insect cell lines, to further enhance the productivity of your protein. Or use our free rare codon analysis tool to see if your gene sequence is suboptimal, and explore means to boost your target protein expression. Learn more

  •          BacuVanceTM      Optimization   Package
  • Standard Package
  • Baculovirus Preparation
  • Case Studies
BacuVanceTM Optimization Protein Expression Package (SC1347)

For customers who wish to establish the best possible protocol for their project and then scale up, GenScript recommends leverage the sophisticated BacuVanceTM platform to explore different conditions for the expression of the target proteins.

Milestones Specifications Deliverables Timeline*
1. Subcloning
  • Subcloning into three expression vectors
Construct(s) report
2 weeks
2. Virus generation
  • Generation of recombinant Bacmid DNA
  • Transfection of insect cell with recombinant Bacmid DNA
  • Generation of P1 stock (Low titer), P2 stock (High titer), P3 determine pfu
5 ml, >107 pfu/ml virus stock
3–4 weeks
3. Expresion optimization and pilot protein production
  • Infection of insect cells with P3 stock
  • Expression optimization and detection:
    MOI, time course, cell lines
  • SDS-PAGE gel and Western blot
Expression data
1.5 weeks
4. Expression and purification
  • 1 L insect cell expression and purification
  • One-step purification
Purified proteins
QC data
1–2 weeks

*Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.

Quotations and Ordering:

protein service quotation

protein services To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

protein services To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

protein services If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.

protein services For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Order by mail Email: protein@genscript.com
Order by phone Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Order by fax Fax: 1-732-210-0262 1-732-885-5878