Baculovirus Expression System

The Baculovirus Expression Vector System (BEVS) is universally recognized as a powerful and versatile tool for the production of large quantities of recombinant proteins due to its ease of use, high expression level, and ability to incorporate post-translational modifications. The GenScript baculovirus expression service package includes both virus production and large-scale protein production, suitable for the pharmaceutical and biotechnology industries and academic institutions. GenScript has pioneered methods for the secretion of recombinant proteins from baculovirus-infected insect cells. We can produce milligram amounts of recombinant protein, suitable for functional screening and biological testing using either the customer's or our own purification processes. Our flexible services have considerable scale-up capacity: We can produce up to 500 liters per project.

GenScript protein expression specialists work closely with clients to clone and express full-length recombinant proteins from full-length cDNAs, EST sequence information, and genomic sequences, providing service and expertise at all stages of protein expression and production.

Advantages of Baculovirus Expression:

Cloning of the gene (with or without affinity tags) into a commercially available expression vector or a custom vector
Production of recombinant viruses using Bac-to-Bac system
Extraction of viral DNA
Amplification and titration of high-titer recombinant virus
Optimization of foreign gene expression and protein production
Scale-up of virus inoculums and protein production in suspension (wave mixer up to 100 L and shake cultures up to 10 L)
¹⁵N- or ¹³C-labeled protein expression

Service Specifications:

Services	Description	Deliverables
I. Generation of recombinant bacmid DNA	Amplification/isolation of the gene or synthetic gene of interest into a baculoviral expression vector Transformation of the expression clone into DH10Bac cells Isolation of recombinant bacmid DNA	Expression vector with insert (5 ug) Verified sequence file Glycerol stock with vector Documentation of procedures
II.Generation of a low-titer viral stock	Transfection of insect cells (Sf9,Sf21,Hi5) with the recombinant bacmid DNA Testing for expression of the recombinant protein by western blot	Expression data Plaque-purified BV virus stock with titer Documentation of expression condition and analysis
III. Amplification of recombinant baculovirus and generation of a high-titer viral stock	Generation of 10 - 200 ml of high-titer viral stock from low-titer stock of a single recombinant Determination of virus titer using qPCR Confirmation of virus titer by plaque assay
IV. Large-scale protein expression	Infection of insect cells with high-titer virus stocks Post-transfectional harvest of insect cells and supernatant	Cell pellet Expression data
V. Protein purification	Protein purification using Hisx6-tag or GST-tag, MBP-tag, DYKDDDDK-tag under native or denaturing conditions Identification of the purified protein by western blot Dialysis	Purified proteins QC data Documentation of procedures
VI. Extras	Tag removal followed by purification with ion-exchange chromatography, gel filtration or reverse-FPLC	Tag-free proteins Documentation of procedures

Quotations and Ordering:

To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure web server.

If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.

For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure web server.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

	Email: protein@genscript.com
	Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
	Fax: 1-732-210-0262 1-732-885-5878