Yeast Expression System

GenScript's yeast expression system combines the best features of bacterial and eukaryotic expression systems. It is ideal for the large-scale production of recombinant eukaryotic proteins. Our yeast expression system incorporates high yield, high productivity, chemically defined media, and mammalian-like product processing with stable and durable production strains to dramatically lower protein production costs.

GenScript employs a transgenic yeast strain to glycosylate recombinant proteins in the same manner as mammalian cells, making it practical to produce proteins with increased applicability in human therapies. This yeast expression system also provides creative and reliable approach to the expression of proteins that are difficult or impossible to express in other systems.

Advantages of Yeast Expression:

Rapid transient expression
Superior expression level
Controllable process
Mammalian-like proteins

Generations of stability
Durability
Cost-effective expression
Maximum value

Services Included:

Subcloning of the gene of interest into a yeast expression vector
Transformation of yeast and selection of transformants
Screening of positive transformants by PCR and verification integration of the gene
Verification of expression of the gene of interest by blot analysis
Pilot expression analysis and optimization
Optimization of expression and fermentation
Small-scale expression from one to five liters
Scale-up expression and fermentation
¹⁵N- or ¹³C-labeled protein expression

Service Specifications:

Services	Description	Deliverables
I. Generation of recombinant yeast vector	Amplification/isolation of target gene or synthetic gene of interest and cloning into yeast expression vector Transformation of the expression clone into yeast and selection of transformants	Expression vector with insert (5 µg) Verified sequence file Glycerol stock with vector Documentation of procedures
II. Pilot expression analysis and optimization	Screening of positive transformants by PCR to verify gene integration Testing for expression of the recombinant protein by western blot analysis	Expression data Documentation of expression condition and analysis
III. Small-scale expression from one to five liters	Verification of gene expression by SDS-PAGE and western analysis Small-scale expression from one to five liters	Cell pellet Expression data
IV. Large-scale protein production and purification	Large-scale production up to 100 L Protein purification by affinity column, ion exchange, gel filtration, and hydrophobic column Removal of tags	Yeast paste, from 10 L to three metric tons Purified proteins QC data Documentation of procedures

Quotations and Ordering:

To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure web server.

If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.

For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure web server.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

	Email: protein@genscript.com
	Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
	Fax: 1-732-210-0262 1-732-885-5878