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Recombinant Protein Services

GenScript can take your project directly from gene synthesis to protein production! Use our free rare codon analysis tool to see if your gene sequence is suboptimal, and explore means to boost your target protein expression.

recombinant protein expression recombinant protein expression-guaranteed package recombinant protein expression - Large-scale Protein Production Services BioProcess Services E.coli Expression Yeast Expression Baculovirus Expression Mammalian Expression

"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service."

— Dr. Barry Bradford, Kansas State University Department of Animal Sciences & Industry


  • Services
  • Competitive Advantages
  • Case Studies
  • Customer Publications

1. Gene-to-Protein: Guaranteed 10 mg purified soluble protein from $2,990 (Cat. No. SC1484)

Package includes:
Deliverables:
Timeline:
  • Gene synthesis
  • Subcloning
  • Expression optimization and verification
  • Protein expression and purification
  • QC by SDS-PAGE, Western blot*, and MALDI-TOF**
  • Gene in pUC57
  • 10 mg purified soluble tagged protein
  • QC data
4–8 weeks

2. Most popular: Guaranteed Protein Expression Package—3 mg purified soluble protein from $2,200 (Cat. No. SC1253)

Package includes:
Deliverables:
Timeline:
  • Subcloning
  • Expression optimization and verification
  • Protein expression and purification
  • QC by SDS-PAGE, Western blot*, and MALDI-TOF**
  • 3 mg or more of purified soluble tagged protein
  • QC data
4–5 weeks

3. BacPowerTM Bacterial Expression System (Cat. No. SC1256)

Specifications:
Deliverables:
Timeline:
  • Subcloning; Expression evaluation and optimization
  • SDS-PAGE and Western blot analysis
  • One-step affinity or multiple steps of purification, protein purity available from 75% to >95%
  • QC by SDS-PAGE, Western blot*, and MALDI-TOF**
  • Tag removal and Endotoxin removal (Optional)
  • Purified soluble protein
  • QC data
4–6 weeks

4. YeastHIGHTM Yeast Expression System (Advanced Package: SC1346/Standard Package: SC1257)

Specifications:
Deliverables:
Timeline:
  • Subcloning; Generation of recombinant yeast vectors
  • Expression evaluation and optimization in various host strains
  • One-step affinity or multiple steps of purification
  • QC by SDS-PAGE, Western blot*, and MALDI-TOF**
  • Cell pellet or purified soluble protein
  • QC data
6–9 weeks

5. BacuVanceTM Baculovirus Expression System (Optimization Package: SC1347/Standard Package: SC1258)

Specifications:
Deliverables:
Timeline:
  • Subcloning; Generation of recombinant bacmid DNA and high-titer viral stock
  • Determination and confirmation of virus titer using qPCR or plaque assay
  • Infection of insect cells (Sf9/Sf21/Hi5) with high-titer virus stocks
  • One-step affinity or multiple steps of purification
  • QC by SDS-PAGE, Western blot*, and MALDI-TOF**
  • High-titer viral stock
  • Cell pellet / media or purified soluble protein
  • QC data
6.5–8.5 weeks

6. Mammalian Expression System (Cat. No. SC1259/SC1260)

Capacity:
Deliverables:
Timeline:
  • Fusion protein or tag-free protein
  • stable cell lines: 3×3 clones
  • QC data
6–8 weeks /Assessed by project

* FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply.

** Optional MALDI-TOF data is available with additional charge.

  1. OptimumGeneTM codon optimization technology for gene expression
GenScript's proprietary OptimumGeneTM Codon Optimization Technology can significantly increase the expression level of target proteins in most systems. Our advanced optimization takes the entire mRNA structure into consideration, as opposed to traditional optimization methodologies which only consider parts of the mRNA structure. The patent-pending OptimumGeneTM Codon Optimization Technology can typically increase the protein expression level by 10-fold in the E. coli system.
View examples

2. Success rate and Extensive recombinant protein production experience:
We have delivered thousands of difficult recombinant proteins including trans-membrane proteins, secreted proteins, proteases, and other enzymes at success rate of >90%. The recombinant proteins we produced have been successfully used in target validation, enzyme characterization, antibody production, high-throughput screening (HTS), structural study, and SAR (Structure-activity relationships).

3. BacPowerTM technology and FoldArtTM protein refolding technology
Hard-to-express and hard-to-dissolve recombinant proteins are specifically tackled by our proprietary BacPowerTM technology and FoldArtTM protein refolding technology.

4. Comprehensive services:
GenScript can take your project directly from gene synthesis, to recombinant protein expression, stable cell line generation, antibody production and assay development.

5. High quality services:
GenScript provides high quality recombinant protein expression services to meet the requirements of high purity, high activity and speedy delivery. Our deliverables are available at the purity of >95%, and the endotoxin level of lower than 0.01 EU/µg.

1. Recombinant protein with biological activity:

Production and purification of proteins with biological activity is essential in drug discovery. Multifunctional units at GenScript secure successful delivery of bioactive recombinant proteins based on our state-of-the-art equipment and methodologies. A case study below shows groups of protein production and assay development teaming up to deliver enzyme with qualified specific activity, higher than customer expected. After modifying the method of cell-breaking, we increase the final yield of recombinant protein of interest with purity of above 90%.

Biological Activity Recombinant Proteins
Fig. A : SDS-PAGE analysis of the fractions upon the IEX chromatography
Lane 1-47: Gradient elution fractions
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0906)
Lane S: The supernatant after homogenizer with high pressure
Lane ft : Flow through
Biological Activity Recombinant Proteins
Fig. B: Enzyme assay monitoring the fractionation by DEAD Sepharose
Biological Activity Recombinant Proteins
Fig. C: Fractions pooled based on activity analysis for further ADP Sepharose affinity purification
Fig. D: SDS-PAGE analysis of the purified protein
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0906)
Lane S: The supernatant after homogenizer with high pressure
Lane ft : Flow through


2. Co-expression of protein complexes

GenScript has successfully co-expressed 3 subunits of A protein in E. coli system:

Co-expression of protein complexes
Fig. A protein with His-tag was analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0906)
Lane 1: A-His-αβγ target protein with His-tag

3. Protein refolding

Overproduction of proteins in E.coli can cause the formation of inactive, misfolded and insoluble protein aggregates. GenScript provides proprietary refolding technology to solve this problem. A case study shows that over 95% of the inclusion body is solublized and refolded. The purity of the refolded recombinant protein is more than 85%. The refolded recombinant protein is delivered in customized buffer.

Protein Refolding
Fig. Refolded protein of sample was analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0900)
Lane 1: Refolded protein of sample

4. Tag removal

Presence of epitope tag in recombinant protein may result in changes in protein structure, toxicity, and loss of bioactivity. To avoid these problems, tag removal after recombinant protein expression is one of additional requests from some customers. Shown below is a recombinant protein that is cleaved by SUMO protease and successfully isolated. About 80% tag free target protein with purity of >90% is recovered.

Tag removal
SDS-PAGE checking purification of a recombinant protein after tag cleavage by SUMO protease
Fig. A:
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat.No. MM0900)
Lane 1: Refolded fusion protein with SUMO-tag
Fig. B:
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat.No. MM0900)
Lane 1: Recovered protein after 1st incubation with Ni-resin
Lane 2: Recovered protein after 2nd incubation with Ni-resin
Lane 3: Elute with 300 mM imidazole from Ni-resin of 2nd incubation

Partial list of publications cited the choice of our protein services

  • Youngjin Park, Mohd Amir F. Abdullah, Milton D. Taylor, Khalidur Rahman, and Michael J. Adang. Enhancement of Bacillus thuringiensis Cry3Aa and Cry3Bb toxicities to coleopteran larvae by a toxin-binding fragment of an insect cadherin. Appl. Envir. Microbiol. May 2009; 75(10): 3086-3092

  • Ilene M. Pedroso, William Hayward, and Terace M. Fletcher. The effect of the TRF2 N-terminal and TRFH regions on telomeric G-quadruplex structures. Nucleic Acids Res. Apr 2009; 37(5): 1541-1554

  • Huiyong Wei, Dan Huang, Jeff Fortman, Richard Wang, Linyun Shao, and Zheng W. Chen. Coadministration of Cidofovir and Smallpox Vaccine Reduced Vaccination Side Effects but Interfered with Vaccine-Elicited Immune Responses and Immunity to Monkeypox. J. Virol. Jan 2009; 83(2): 1115 - 1125

  • Ashish Nagarsekar, Rachel S. Greenberg, Nirav G. Shah, Ishwar S. Singh, and Jeffrey D. Hasday. Febrile-Range Hyperthermia Accelerates Caspase-Dependent Apoptosis in Human Neutrophils. J. Immunol. Aug 2008; 181(4): 2636 - 2643

  • Brando C, Ware LA, Freyberger H, Kathcart A, Barbosa A, Cayphas S, Demoitie MA, Mettens P, Heppner DG, Lanar DE. Murine immune responses to liver-stage antigen 1 protein FMP011, a malaria vaccine candidate, delivered with adjuvant AS01B or AS02A. Infect. Immun. Feb 2007; 75(2): 838-845

  • Buchko GW, Ni S, Robinson H, Welsh EA, Pakrasi HB, Kennedy MA. Characterization of two potentially universal turn motifs that shape the repeated five-residues fold--crystal structure of a lumenal pentapeptide repeat protein from Cyanothece 51142. Protein Sci. Nov 2006; 15(11): 2579-2595

  • Dieterich DC, Link AJ, Graumann J, Tirrell DA, Schuman EM. Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc. Natl. Acad. Sci. USA. Jun 2006; 103(25): 9482-9487

See more publications following this link...

Quotations and Ordering:

recombinant protein expression quotation

recombinant protein expression To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

recombinant protein expression To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

recombinant protein expression If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Recombinant Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.

recombinant protein expression For questions about recombinant protein expression, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Order by mail Email: protein@genscript.com
recombinant protein expression Phone: 1-877-436-7274 (Toll-Free)　　1-732-885-9188
recombinant protein expression Fax: 1-732-210-0262　　1-732-885-5878