Myo7a gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_011241690.1	XP_011239992.1	unconventional myosin-VIIa isoform X3
XM_006507429.2	XP_006507492.1	unconventional myosin-VIIa isoform X7
XM_011241689.2	XP_011239991.1	unconventional myosin-VIIa isoform X2
XM_011241691.2	XP_011239993.1	unconventional myosin-VIIa isoform X4
NM_001256081.1	NP_001243010.1	unconventional myosin-VIIa isoform 1
XM_011241688.2	XP_011239990.1	unconventional myosin-VIIa isoform X1
XM_006507428.2	XP_006507491.1	unconventional myosin-VIIa isoform X6
XM_011241690.2	XP_011239992.1	unconventional myosin-VIIa isoform X3
XM_006507427.4	XP_006507490.1	unconventional myosin-VIIa isoform X5
NM_001256083.1	NP_001243012.1	unconventional myosin-VIIa isoform 4
XM_011241689.1	XP_011239991.1	unconventional myosin-VIIa isoform X2
XM_011241691.1	XP_011239993.1	unconventional myosin-VIIa isoform X4
XM_011241688.3	XP_011239990.1	unconventional myosin-VIIa isoform X1
NM_008663.2	NP_032689.2	unconventional myosin-VIIa isoform 2
NM_001256082.1	NP_001243011.1	unconventional myosin-VIIa isoform 3
XM_006507427.3	XP_006507490.1	unconventional myosin-VIIa isoform X5
XM_006507428.3	XP_006507491.1	unconventional myosin-VIIa isoform X6
XM_006507429.3	XP_006507492.1	unconventional myosin-VIIa isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20013	NM_001256081.1 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu20144	XM_011241690.2 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu20201	XM_011241691.2 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39357	XM_006507427.4 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39358	XM_006507428.3 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu39359	XM_006507429.3 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu68262	XM_011241688.3 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu68263	XM_011241689.2 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu20144	NM_001256082.1 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu20162	NM_008663.2 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1602.30 $2289.00
OMu20201	NM_001256083.1 Latest version!	Mus musculus myosin VIIA (Myo7a), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu20144	XM_011241690.1	Mus musculus myosin VIIA (Myo7a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu68263	XM_011241689.1	Mus musculus myosin VIIA (Myo7a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu20201	XM_011241691.1	Mus musculus myosin VIIA (Myo7a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu68262	XM_011241688.2	Mus musculus myosin VIIA (Myo7a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39357	XM_006507427.3	Mus musculus myosin VIIA (Myo7a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39358	XM_006507428.2	Mus musculus myosin VIIA (Myo7a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu39359	XM_006507429.2	Mus musculus myosin VIIA (Myo7a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

Myo7a ( NM_001256081.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243010.1 Mus musculus myosin VIIA (Myo7a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20013
Clone ID Related Accession (Same CDS sequence)	NM_001256081.1
Accession Version	NM_001256081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115022.11 and AC157792.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U81453.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACGTA CACAGGTTCC 
ATCCTGGTGG CCGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTACACCA ACAAGAAGAT AGGGGAGATG CCCCCCCACA TCTTCGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAACCGG GACCAGTGCT GTATTATCAG CGGGGAGTCG 
GGAGCTGGCA AGACAGAGAG CACAAAGTTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGA 
CAGCACTCAT GGATCGAGCA GCAGGTGCTG GAGGCCACCC CGATCCTGGA AGCATTTGGG 
AACGCCAAGA CCATCCGCAA CGACAACTCT AGCCGCTTTG GCAAGTACAT TGACATCCAC 
TTTAACAAGC GTGGTGCCAT CGAGGGCGCC AAAATAGAGC AATACCTGCT GGAGAAGTCA 
CGTGTCTGCC GCCAGGCCCC TGACGAGAGG AACTATCACG TGTTCTACTG TATGCTGGAG 
GGCATGAATG AGGAGGAGAA GAAGAAACTG GGCCTAGGCC AGGCCGCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT CACCTGTGAG GGCCGCGTGG ACAGTCAGGA GTATGCCAAC 
ATCCGCTCTG CCATGAAGGT TCTCATGTTC ACAGACACGG AGAACTGGGA GATCTCGAAG 
CTTCTGGCTG CCATCCTACA CATGGGCAAT CTGCAGTATG AGGCCCGGAC ATTTGAGAAC 
TTGGATGCGT GTGAAGTCCT CTTCTCCCCA TCGCTGGCCA CGGCAGCTTC TCTGCTCGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTC ACCAGCCGCA CCCTCATCAC CCGTGGGGAG 
ACGGTGTCCA CCCCTCTCAG CAGGGAACAG GCGCTGGATG TGCGAGATGC CTTTGTCAAG 
GGCATCTATG GGCGGCTCTT TGTGTGGATT GTGGAGAAGA TCAACGCAGC AATCTACAAG 
CCACCCCCCC TGGAAGTGAA GAACTCTCGC CGGTCCATCG GTCTCCTGGA CATCTTTGGA 
TTTGAGAACT TCACTGTGAA CAGCTTCGAG CAGCTCTGCA TTAACTTTGC CAATGAGCAC 
CTGCAGCAAT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA CGACCTGGAG 
AGCATCGACT GGTTGCACAT TGAGTTCACT GACAACCAGG AAGCACTGGA CATGATTGCC 
AACCGGCCTA TGAACGTCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG 
GATGCCACCA TGCTGCATAA GCTGAACTCA CAGCACAAGC TCAATGCCAA CTACGTGCCA 
CCCAAGAACA GCCACGAGAC CCAGTTTGGA ATCAACCACT TTGCGGGTGT TGTCTATTAT 
GAGAGTCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT CATCCAGCTG 
GTCCACTCTT CCCGGAACAA GTTCATAAAG CAGATTTTCC AAGCTGACGT TGCCATGGGT 
GCCGAGACCA GGAAGCGCTC GCCTACACTC AGCAGCCAGT TCAAGCGGTC TCTGGAGCTG 
CTGATGCGCA CACTGGGCGC CTGCCAGCCC TTCTTTGTGC GTTGTATCAA ACCCAATGAG 
TTCAAGAAGC CCATGCTCTT CGACCGGCAC TTGTGTGTAC GCCAGCTGCG ATATTCGGGC 
ATGATGGAGA CAATCCGCAT CCGCCACGCA GGCTACCCCA TTCGCTACAG CTTTGTGGAG 
TTTGTGGAGC GCTACCGGGT ACTGCTGCCT GGTGTGAAGC CAGCATACAA GCAGGGTGAC 
CTCCGAGGGA CATGCCAGCG CATGGCTGAG GCTGTGCTGG GCACGCACGA TGACTGGCAG 
ATTGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGTTGCTGGA GGTGGAGCGG 
GACAAGGCCA TCACAGACAG AGTCATTCTC CTCCAGAAGG TTATCCGGGG CTTCAAAGAC 
AGGTCCAACT TCCTGAGACT GAAGAGTGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC 
CACCACTGTA GGAAAAACTA TGAGCTGATT CGTCTTGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CAAGCAGTAC CGCCTGGCCA GACAGCGCAT AATAGAGTTC 
CAGGCCCGCT GCCGGGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG 
ATCACCGTGC AGGCCTATGC CCGAGGCATG ATTGCCCGCC GGCTACACCG CCGCCTCCGG 
GTTGAGTACC AGCGGCGCCT CGAGGCAGAG AGGATGCGTC TGGCAGAGGA GGAGAAACTC 
CGAAAGGAGA TGAGTGCCAA GAAGGCCAAA GAGGAGGCTG AGCGCAAGCA TCAGGAGCGC 
CTGGCTCAGC TAGCCCGCGA GGATGCGGAG CGGGAACTGA AGGAGAAGGA GGAGGCTCGG 
AGGAAGAAGG AACTGCTGGA GCAGATGGAG AAGGCCCGCC ACGAACCCAT CAACCACTCA 
GATATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GCAGCCTGCC AGGCCAGGAA 
GGCCAGGCGC CTAGTGGCTT TGAGGACCTA GAGCGCGGAC GGAGGGAGAT GGTGGAAGAG 
GATGTTGACG CTGCCCTGCC CCTGCCTGAT GAAGACGAGG AGGACCTTTC TGAGTACAAA 
TTCGCCAAGT TTGCTGCCAC CTACTTCCAG GGCACAACCA CACACTCCTA CACCCGGAGG 
CCTCTCAAGC AGCCGCTGCT CTACCACGAC GATGAGGGTG ACCAGCTGGC GGCGCTGGCT 
GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAAGTA CCACACAGCC 
ATGAGCGACG GCAGTGAGAA GATCCCAGTG ATGACTAAGA TCTACGAGAC CCTAGGCAAG 
AAGACATATA AGAGGGAGCT GCAGGCCTTG CAGGGCGAGG GCGAGACCCA GCTCCCTGAG 
GGGCAGAAGA AGACCAGTGT GAGACACAAG TTGGTACACT TGACACTGAA GAAAAAGTCC 
AAACTCACAG AAGAGGTGAC CAAGAGGCTG AACGATGGGG AATCCACGGT ACAGGGCAAC 
AGCATGCTGG AGGATCGGCC CACCTCAAAT CTAGAGAAGC TGCACTTCAT CATCGGCAAC 
GGCATCCTGC GGCCTGCGCT GCGGGACGAG ATTTACTGCC AGATCAGTAA GCAGCTCACA 
CACAACCCAT CCAAGAGCAG CTATGCCAGG GGCTGGATCC TCGTGTCGCT CTGTGTGGGC 
TGCTTCGCCC CCTCTGAGAA GTTCGTTAAG TACCTGCGGA ACTTCATCCA CGGAGGCCCA 
CCTGGCTATG CTCCTTACTG TGAGGAGCGC CTGAGGAGGA CCTTTGTCAA CGGAACTCGG 
ACACAGCCAC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG 
CCCGTGACCT TCATGGATGG GACCACCAAG ACCCTGCTAA CAGATTCAGC AACTACAGCC 
AGGGAGCTGT GCAATGCTCT GGCTGACAAG ATCTCACTCA AGGACCGCTT TGGCTTCTCC 
CTCTACATCG CTCTGTTCGA TAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCATGTCATG 
GATGCCATCT CTCAGTGTGA GCAGTACGCC AAGGAGCAGG GTGCTCAGGA GCGCAACGCC 
CCATGGAGGC TCTTCTTTAG AAAGGAGGTC TTCACACCCT GGCACAACCC CTCGGAGGAC 
AACGTGGCCA CGAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCTTCTC AGCAGTACTT TGTGGACTAT 
GGTTCTGAGA TGATTCTGGA GCGCCTGCTG AGCCTCGTGC CCACTTACAT CCCTGACCGT 
GAGATCACAC CGCTGAAGAA TCTTGAGAAG TGGGCACAGC TGGCCATTGC TGCCCACAAG 
AAGGGAATTT ATGCCCAGAG GAGAACTGAC TCCCAGAAGG TCAAAGAGGA TGTGGTCAAT 
TATGCCCGTT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ACGAAGCTTA CAAATTCTCA 
GGCCCTCCCC TCCCCAAGAG CGACGTCATC GTGGCTGTCA ACTGGACGGG TGTGTACTTC 
GTGGACGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CGGAGATCAT GGCTGTGTCC 
AGCAGTAGGG AGTGCCGCGT CTTGCTCTCA CTGGGCTGCT CTGACTTGGG CTGTGCTACT 
TGTCAATCGG GCCGGGCAGG GCTGACCCCG GCTGGACCCT GTTCTCCGTG TTGGTCCTGT 
AGGGGAACAA AGATGATGGC CCCCAGCTTT ACCCTGGCCA CCATCAAAGG AGATGAGTAC 
ACCTTCACAT CCAGCAATGC TGAGGACATC CGTGACCTGG TGGTCACCTT TCTGGAGGGG 
CTACGGAAGA GGTCTAAGTA TGTGGTGGCA CTGCAGGACA ATCCTAACCC TGCTGGTGAG 
GAGTCAGGCT TCCTCAGCTT CGCCAAGGGA GACCTCATCA TCCTTGACCA TGATACTGGT 
GAGCAGGTCA TGAACTCAGG CTGGGCCAAC GGCATCAACG AGAGGACCAA GCAGCGCGGC 
GACTTCCCCA CTGACTGTGT ATACGTCATG CCCACTGTCA CCTTGCCACC AAGGGAGATT 
GTGGCCCTGG TCACTATGAC CCCAGACCAG AGGCAGGATG TCGTCCGGCT CCTGCAGCTT 
CGCACAGCAG AGCCAGAGGT GCGCGCCAAG CCCTACACGC TAGAGGAGTT CTCCTACGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGCGGT 
AAGGACAGGC TGTGGAGCCA CACACGAGAG CCCCTCAAGC AGGCCCTGCT CAAGAAGATC 
CTGGGCAGTG AAGAACTCTC CCAGGAAGCC TGCATGGCCT TTGTAGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCATCCAA GAGGATGCGA TCCGTCAATG AGCTCACTGA CCAGATCTTT 
GAGTGGGCAC TCAAGGCTGA GCCCCTCAAG GATGAGGCCT ACGTGCAGAT CCTGAAGCAG 
CTGACTGACA ATCACATCAG GTACAGCGAA GAGAGGGGCT GGGAACTGCT GTGGCTGTGC 
ACGGGCCTCT TCCCGCCCAG CAACATCCTC CTGCCTCATG TTCAGCGGTT TCTGCAGTCC 
CGCAAGCACT GTCCTCTTGC CATTGACTGC CTGCAGAGGC TCCAGAAAGC CCTGAGAAAT 
GGCTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAACA TAAGACTACC 
CAGATCTTCC ACAAGGTCTA CTTCCCCGAT GACACGGACG AGGCTTTTGA GGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCAGCC GGCTGCTGCT CAAGTCTTCC 
GAGGGATTCA GCCTTTTTGT CAAAATCGCA GATAAGGTCA TCAGCGTCCC AGAGAATGAT 
TTCTTCTTTG ACTTTGTCCG ACACCTGACA GACTGGATAA AGAAAGCACG GCCCATCAAG 
GACGGAATCG TGCCCTCACT AACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACAGTGCCGG GCAAGGACCC CATGGCTGAC TCCATCTTCC ACTATTACCA GGAACTGCCC 
AAATATCTCC GAGGCTACCA CAAGTGCACC CGGGAGGAGG TGCTGCAGCT GGGCGCACTC 
ATCTACAGGG TCAAGTTTGA GGAGGACAAA TCCTACTTCC CTAGCATCCC CAAGTTGCTG 
AGGGAGCTGG TACCCCAGGA CCTAATCCGG CAGGTCTCAC CTGATGACTG GAAACGGTCT 
ATTGTCGCCT ACTTCAACAA ACATGCGGGG AAGTCCAAGG AGGAAGCCAA GCTGGCCTTC 
CTCAAACTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTTGAGGT GAAGCAAACT 
ACAGAACCAA ACTTCCCAGA GATTCTCTTA ATTGCCATCA ACAAGTACGG GGTCAGCCTC 
ATCGATCCCA GAACCAAGGA CATCCTGACT ACTCACCCCT TCACCAAGAT CTCCAACTGG 
AGTAGTGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTCCGTGG GAGCAAACTG 
CTCTGTGAGA CATCGCTGGG ATACAAAATG GATGATCTTC TGACTTCCTA CATCAGCCAG 
ATGCTCACAG CCATGAGCAA GCAGAGGAAC TCCAGGAGTG GAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243010.1
CDS	261..6908
Translation	MVILQKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	NM_001256081.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256081.1

ORF Insert Sequence:

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACGTA CACAGGTTCC 
ATCCTGGTGG CCGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTACACCA ACAAGAAGAT AGGGGAGATG CCCCCCCACA TCTTCGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAACCGG GACCAGTGCT GTATTATCAG CGGGGAGTCG 
GGAGCTGGCA AGACAGAGAG CACAAAGTTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGA 
CAGCACTCAT GGATCGAGCA GCAGGTGCTG GAGGCCACCC CGATCCTGGA AGCATTTGGG 
AACGCCAAGA CCATCCGCAA CGACAACTCT AGCCGCTTTG GCAAGTACAT TGACATCCAC 
TTTAACAAGC GTGGTGCCAT CGAGGGCGCC AAAATAGAGC AATACCTGCT GGAGAAGTCA 
CGTGTCTGCC GCCAGGCCCC TGACGAGAGG AACTATCACG TGTTCTACTG TATGCTGGAG 
GGCATGAATG AGGAGGAGAA GAAGAAACTG GGCCTAGGCC AGGCCGCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT CACCTGTGAG GGCCGCGTGG ACAGTCAGGA GTATGCCAAC 
ATCCGCTCTG CCATGAAGGT TCTCATGTTC ACAGACACGG AGAACTGGGA GATCTCGAAG 
CTTCTGGCTG CCATCCTACA CATGGGCAAT CTGCAGTATG AGGCCCGGAC ATTTGAGAAC 
TTGGATGCGT GTGAAGTCCT CTTCTCCCCA TCGCTGGCCA CGGCAGCTTC TCTGCTCGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTC ACCAGCCGCA CCCTCATCAC CCGTGGGGAG 
ACGGTGTCCA CCCCTCTCAG CAGGGAACAG GCGCTGGATG TGCGAGATGC CTTTGTCAAG 
GGCATCTATG GGCGGCTCTT TGTGTGGATT GTGGAGAAGA TCAACGCAGC AATCTACAAG 
CCACCCCCCC TGGAAGTGAA GAACTCTCGC CGGTCCATCG GTCTCCTGGA CATCTTTGGA 
TTTGAGAACT TCACTGTGAA CAGCTTCGAG CAGCTCTGCA TTAACTTTGC CAATGAGCAC 
CTGCAGCAAT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA CGACCTGGAG 
AGCATCGACT GGTTGCACAT TGAGTTCACT GACAACCAGG AAGCACTGGA CATGATTGCC 
AACCGGCCTA TGAACGTCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG 
GATGCCACCA TGCTGCATAA GCTGAACTCA CAGCACAAGC TCAATGCCAA CTACGTGCCA 
CCCAAGAACA GCCACGAGAC CCAGTTTGGA ATCAACCACT TTGCGGGTGT TGTCTATTAT 
GAGAGTCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT CATCCAGCTG 
GTCCACTCTT CCCGGAACAA GTTCATAAAG CAGATTTTCC AAGCTGACGT TGCCATGGGT 
GCCGAGACCA GGAAGCGCTC GCCTACACTC AGCAGCCAGT TCAAGCGGTC TCTGGAGCTG 
CTGATGCGCA CACTGGGCGC CTGCCAGCCC TTCTTTGTGC GTTGTATCAA ACCCAATGAG 
TTCAAGAAGC CCATGCTCTT CGACCGGCAC TTGTGTGTAC GCCAGCTGCG ATATTCGGGC 
ATGATGGAGA CAATCCGCAT CCGCCACGCA GGCTACCCCA TTCGCTACAG CTTTGTGGAG 
TTTGTGGAGC GCTACCGGGT ACTGCTGCCT GGTGTGAAGC CAGCATACAA GCAGGGTGAC 
CTCCGAGGGA CATGCCAGCG CATGGCTGAG GCTGTGCTGG GCACGCACGA TGACTGGCAG 
ATTGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGTTGCTGGA GGTGGAGCGG 
GACAAGGCCA TCACAGACAG AGTCATTCTC CTCCAGAAGG TTATCCGGGG CTTCAAAGAC 
AGGTCCAACT TCCTGAGACT GAAGAGTGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC 
CACCACTGTA GGAAAAACTA TGAGCTGATT CGTCTTGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CAAGCAGTAC CGCCTGGCCA GACAGCGCAT AATAGAGTTC 
CAGGCCCGCT GCCGGGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG 
ATCACCGTGC AGGCCTATGC CCGAGGCATG ATTGCCCGCC GGCTACACCG CCGCCTCCGG 
GTTGAGTACC AGCGGCGCCT CGAGGCAGAG AGGATGCGTC TGGCAGAGGA GGAGAAACTC 
CGAAAGGAGA TGAGTGCCAA GAAGGCCAAA GAGGAGGCTG AGCGCAAGCA TCAGGAGCGC 
CTGGCTCAGC TAGCCCGCGA GGATGCGGAG CGGGAACTGA AGGAGAAGGA GGAGGCTCGG 
AGGAAGAAGG AACTGCTGGA GCAGATGGAG AAGGCCCGCC ACGAACCCAT CAACCACTCA 
GATATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GCAGCCTGCC AGGCCAGGAA 
GGCCAGGCGC CTAGTGGCTT TGAGGACCTA GAGCGCGGAC GGAGGGAGAT GGTGGAAGAG 
GATGTTGACG CTGCCCTGCC CCTGCCTGAT GAAGACGAGG AGGACCTTTC TGAGTACAAA 
TTCGCCAAGT TTGCTGCCAC CTACTTCCAG GGCACAACCA CACACTCCTA CACCCGGAGG 
CCTCTCAAGC AGCCGCTGCT CTACCACGAC GATGAGGGTG ACCAGCTGGC GGCGCTGGCT 
GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAAGTA CCACACAGCC 
ATGAGCGACG GCAGTGAGAA GATCCCAGTG ATGACTAAGA TCTACGAGAC CCTAGGCAAG 
AAGACATATA AGAGGGAGCT GCAGGCCTTG CAGGGCGAGG GCGAGACCCA GCTCCCTGAG 
GGGCAGAAGA AGACCAGTGT GAGACACAAG TTGGTACACT TGACACTGAA GAAAAAGTCC 
AAACTCACAG AAGAGGTGAC CAAGAGGCTG AACGATGGGG AATCCACGGT ACAGGGCAAC 
AGCATGCTGG AGGATCGGCC CACCTCAAAT CTAGAGAAGC TGCACTTCAT CATCGGCAAC 
GGCATCCTGC GGCCTGCGCT GCGGGACGAG ATTTACTGCC AGATCAGTAA GCAGCTCACA 
CACAACCCAT CCAAGAGCAG CTATGCCAGG GGCTGGATCC TCGTGTCGCT CTGTGTGGGC 
TGCTTCGCCC CCTCTGAGAA GTTCGTTAAG TACCTGCGGA ACTTCATCCA CGGAGGCCCA 
CCTGGCTATG CTCCTTACTG TGAGGAGCGC CTGAGGAGGA CCTTTGTCAA CGGAACTCGG 
ACACAGCCAC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG 
CCCGTGACCT TCATGGATGG GACCACCAAG ACCCTGCTAA CAGATTCAGC AACTACAGCC 
AGGGAGCTGT GCAATGCTCT GGCTGACAAG ATCTCACTCA AGGACCGCTT TGGCTTCTCC 
CTCTACATCG CTCTGTTCGA TAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCATGTCATG 
GATGCCATCT CTCAGTGTGA GCAGTACGCC AAGGAGCAGG GTGCTCAGGA GCGCAACGCC 
CCATGGAGGC TCTTCTTTAG AAAGGAGGTC TTCACACCCT GGCACAACCC CTCGGAGGAC 
AACGTGGCCA CGAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCTTCTC AGCAGTACTT TGTGGACTAT 
GGTTCTGAGA TGATTCTGGA GCGCCTGCTG AGCCTCGTGC CCACTTACAT CCCTGACCGT 
GAGATCACAC CGCTGAAGAA TCTTGAGAAG TGGGCACAGC TGGCCATTGC TGCCCACAAG 
AAGGGAATTT ATGCCCAGAG GAGAACTGAC TCCCAGAAGG TCAAAGAGGA TGTGGTCAAT 
TATGCCCGTT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ACGAAGCTTA CAAATTCTCA 
GGCCCTCCCC TCCCCAAGAG CGACGTCATC GTGGCTGTCA ACTGGACGGG TGTGTACTTC 
GTGGACGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CGGAGATCAT GGCTGTGTCC 
AGCAGTAGGG AGTGCCGCGT CTTGCTCTCA CTGGGCTGCT CTGACTTGGG CTGTGCTACT 
TGTCAATCGG GCCGGGCAGG GCTGACCCCG GCTGGACCCT GTTCTCCGTG TTGGTCCTGT 
AGGGGAACAA AGATGATGGC CCCCAGCTTT ACCCTGGCCA CCATCAAAGG AGATGAGTAC 
ACCTTCACAT CCAGCAATGC TGAGGACATC CGTGACCTGG TGGTCACCTT TCTGGAGGGG 
CTACGGAAGA GGTCTAAGTA TGTGGTGGCA CTGCAGGACA ATCCTAACCC TGCTGGTGAG 
GAGTCAGGCT TCCTCAGCTT CGCCAAGGGA GACCTCATCA TCCTTGACCA TGATACTGGT 
GAGCAGGTCA TGAACTCAGG CTGGGCCAAC GGCATCAACG AGAGGACCAA GCAGCGCGGC 
GACTTCCCCA CTGACTGTGT ATACGTCATG CCCACTGTCA CCTTGCCACC AAGGGAGATT 
GTGGCCCTGG TCACTATGAC CCCAGACCAG AGGCAGGATG TCGTCCGGCT CCTGCAGCTT 
CGCACAGCAG AGCCAGAGGT GCGCGCCAAG CCCTACACGC TAGAGGAGTT CTCCTACGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGCGGT 
AAGGACAGGC TGTGGAGCCA CACACGAGAG CCCCTCAAGC AGGCCCTGCT CAAGAAGATC 
CTGGGCAGTG AAGAACTCTC CCAGGAAGCC TGCATGGCCT TTGTAGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCATCCAA GAGGATGCGA TCCGTCAATG AGCTCACTGA CCAGATCTTT 
GAGTGGGCAC TCAAGGCTGA GCCCCTCAAG GATGAGGCCT ACGTGCAGAT CCTGAAGCAG 
CTGACTGACA ATCACATCAG GTACAGCGAA GAGAGGGGCT GGGAACTGCT GTGGCTGTGC 
ACGGGCCTCT TCCCGCCCAG CAACATCCTC CTGCCTCATG TTCAGCGGTT TCTGCAGTCC 
CGCAAGCACT GTCCTCTTGC CATTGACTGC CTGCAGAGGC TCCAGAAAGC CCTGAGAAAT 
GGCTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAACA TAAGACTACC 
CAGATCTTCC ACAAGGTCTA CTTCCCCGAT GACACGGACG AGGCTTTTGA GGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCAGCC GGCTGCTGCT CAAGTCTTCC 
GAGGGATTCA GCCTTTTTGT CAAAATCGCA GATAAGGTCA TCAGCGTCCC AGAGAATGAT 
TTCTTCTTTG ACTTTGTCCG ACACCTGACA GACTGGATAA AGAAAGCACG GCCCATCAAG 
GACGGAATCG TGCCCTCACT AACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACAGTGCCGG GCAAGGACCC CATGGCTGAC TCCATCTTCC ACTATTACCA GGAACTGCCC 
AAATATCTCC GAGGCTACCA CAAGTGCACC CGGGAGGAGG TGCTGCAGCT GGGCGCACTC 
ATCTACAGGG TCAAGTTTGA GGAGGACAAA TCCTACTTCC CTAGCATCCC CAAGTTGCTG 
AGGGAGCTGG TACCCCAGGA CCTAATCCGG CAGGTCTCAC CTGATGACTG GAAACGGTCT 
ATTGTCGCCT ACTTCAACAA ACATGCGGGG AAGTCCAAGG AGGAAGCCAA GCTGGCCTTC 
CTCAAACTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTTGAGGT GAAGCAAACT 
ACAGAACCAA ACTTCCCAGA GATTCTCTTA ATTGCCATCA ACAAGTACGG GGTCAGCCTC 
ATCGATCCCA GAACCAAGGA CATCCTGACT ACTCACCCCT TCACCAAGAT CTCCAACTGG 
AGTAGTGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTCCGTGG GAGCAAACTG 
CTCTGTGAGA CATCGCTGGG ATACAAAATG GATGATCTTC TGACTTCCTA CATCAGCCAG 
ATGCTCACAG CCATGAGCAA GCAGAGGAAC TCCAGGAGTG GAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241690.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239992.1 Mus musculus myosin VIIA (Myo7a), transcript variant X3, mRNA.

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CloneID	OMu20144
Clone ID Related Accession (Same CDS sequence)	XM_011241690.1 , XM_011241690.2 , NM_001256082.1
Accession Version	XM_011241690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGGAACA 
AAGATGATGG CCCCCAGCTT TACCCTGGCC ACCATCAAAG GAGATGAGTA CACCTTCACA 
TCCAGCAATG CTGAGGACAT CCGTGACCTG GTGGTCACCT TTCTGGAGGG GCTACGGAAG 
AGGTCTAAGT ATGTGGTGGC ACTGCAGGAC AATCCTAACC CTGCTGGTGA GGAGTCAGGC 
TTCCTCAGCT TCGCCAAGGG AGACCTCATC ATCCTTGACC ATGATACTGG TGAGCAGGTC 
ATGAACTCAG GCTGGGCCAA CGGCATCAAC GAGAGGACCA AGCAGCGCGG CGACTTCCCC 
ACTGACTGTG TATACGTCAT GCCCACTGTC ACCTTGCCAC CAAGGGAGAT TGTGGCCCTG 
GTCACTATGA CCCCAGACCA GAGGCAGGAT GTCGTCCGGC TCCTGCAGCT TCGCACAGCA 
GAGCCAGAGG TGCGCGCCAA GCCCTACACG CTAGAGGAGT TCTCCTACGA CTACTTCAGG 
CCCCCACCCA AGCACACGCT GAGCCGTGTC ATGGTGTCCA AGGCCCGCGG TAAGGACAGG 
CTGTGGAGCC ACACACGAGA GCCCCTCAAG CAGGCCCTGC TCAAGAAGAT CCTGGGCAGT 
GAAGAACTCT CCCAGGAAGC CTGCATGGCC TTTGTAGCTG TGCTCAAGTA CATGGGCGAC 
TACCCATCCA AGAGGATGCG ATCCGTCAAT GAGCTCACTG ACCAGATCTT TGAGTGGGCA 
CTCAAGGCTG AGCCCCTCAA GGATGAGGCC TACGTGCAGA TCCTGAAGCA GCTGACTGAC 
AATCACATCA GGTACAGCGA AGAGAGGGGC TGGGAACTGC TGTGGCTGTG CACGGGCCTC 
TTCCCGCCCA GCAACATCCT CCTGCCTCAT GTTCAGCGGT TTCTGCAGTC CCGCAAGCAC 
TGTCCTCTTG CCATTGACTG CCTGCAGAGG CTCCAGAAAG CCCTGAGAAA TGGCTCCCGG 
AAGTACCCTC CGCACCTGGT GGAGGTGGAG GCCATCCAAC ATAAGACTAC CCAGATCTTC 
CACAAGGTCT ACTTCCCCGA TGACACGGAC GAGGCTTTTG AGGTGGAGTC CAGCACCAAG 
GCCAAGGACT TCTGCCAGAA CATCGCCAGC CGGCTGCTGC TCAAGTCTTC CGAGGGATTC 
AGCCTTTTTG TCAAAATCGC AGATAAGGTC ATCAGCGTCC CAGAGAATGA TTTCTTCTTT 
GACTTTGTCC GACACCTGAC AGACTGGATA AAGAAAGCAC GGCCCATCAA GGACGGAATC 
GTGCCCTCAC TAACCTACCA GGTGTTCTTC ATGAAGAAGC TGTGGACCAC CACAGTGCCG 
GGCAAGGACC CCATGGCTGA CTCCATCTTC CACTATTACC AGGAACTGCC CAAATATCTC 
CGAGGCTACC ACAAGTGCAC CCGGGAGGAG GTGCTGCAGC TGGGCGCACT CATCTACAGG 
GTCAAGTTTG AGGAGGACAA ATCCTACTTC CCTAGCATCC CCAAGTTGCT GAGGGAGCTG 
GTACCCCAGG ACCTAATCCG GCAGGTCTCA CCTGATGACT GGAAACGGTC TATTGTCGCC 
TACTTCAACA AACATGCGGG GAAGTCCAAG GAGGAAGCCA AGCTGGCCTT CCTCAAACTC 
ATCTTCAAGT GGCCCACCTT TGGCTCAGCC TTCTTTGAGG TGAAGCAAAC TACAGAACCA 
AACTTCCCAG AGATTCTCTT AATTGCCATC AACAAGTACG GGGTCAGCCT CATCGATCCC 
AGAACCAAGG ACATCCTGAC TACTCACCCC TTCACCAAGA TCTCCAACTG GAGTAGTGGC 
AACACCTACT TCCACATCAC CATTGGGAAC TTGGTCCGTG GGAGCAAACT GCTCTGTGAG 
ACATCGCTGG GATACAAAAT GGATGATCTT CTGACTTCCT ACATCAGCCA GATGCTCACA 
GCCATGAGCA AGCAGAGGAA CTCCAGGAGT GGAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239992.1
CDS	34..6552
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241690.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241690.2

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGGAACA 
AAGATGATGG CCCCCAGCTT TACCCTGGCC ACCATCAAAG GAGATGAGTA CACCTTCACA 
TCCAGCAATG CTGAGGACAT CCGTGACCTG GTGGTCACCT TTCTGGAGGG GCTACGGAAG 
AGGTCTAAGT ATGTGGTGGC ACTGCAGGAC AATCCTAACC CTGCTGGTGA GGAGTCAGGC 
TTCCTCAGCT TCGCCAAGGG AGACCTCATC ATCCTTGACC ATGATACTGG TGAGCAGGTC 
ATGAACTCAG GCTGGGCCAA CGGCATCAAC GAGAGGACCA AGCAGCGCGG CGACTTCCCC 
ACTGACTGTG TATACGTCAT GCCCACTGTC ACCTTGCCAC CAAGGGAGAT TGTGGCCCTG 
GTCACTATGA CCCCAGACCA GAGGCAGGAT GTCGTCCGGC TCCTGCAGCT TCGCACAGCA 
GAGCCAGAGG TGCGCGCCAA GCCCTACACG CTAGAGGAGT TCTCCTACGA CTACTTCAGG 
CCCCCACCCA AGCACACGCT GAGCCGTGTC ATGGTGTCCA AGGCCCGCGG TAAGGACAGG 
CTGTGGAGCC ACACACGAGA GCCCCTCAAG CAGGCCCTGC TCAAGAAGAT CCTGGGCAGT 
GAAGAACTCT CCCAGGAAGC CTGCATGGCC TTTGTAGCTG TGCTCAAGTA CATGGGCGAC 
TACCCATCCA AGAGGATGCG ATCCGTCAAT GAGCTCACTG ACCAGATCTT TGAGTGGGCA 
CTCAAGGCTG AGCCCCTCAA GGATGAGGCC TACGTGCAGA TCCTGAAGCA GCTGACTGAC 
AATCACATCA GGTACAGCGA AGAGAGGGGC TGGGAACTGC TGTGGCTGTG CACGGGCCTC 
TTCCCGCCCA GCAACATCCT CCTGCCTCAT GTTCAGCGGT TTCTGCAGTC CCGCAAGCAC 
TGTCCTCTTG CCATTGACTG CCTGCAGAGG CTCCAGAAAG CCCTGAGAAA TGGCTCCCGG 
AAGTACCCTC CGCACCTGGT GGAGGTGGAG GCCATCCAAC ATAAGACTAC CCAGATCTTC 
CACAAGGTCT ACTTCCCCGA TGACACGGAC GAGGCTTTTG AGGTGGAGTC CAGCACCAAG 
GCCAAGGACT TCTGCCAGAA CATCGCCAGC CGGCTGCTGC TCAAGTCTTC CGAGGGATTC 
AGCCTTTTTG TCAAAATCGC AGATAAGGTC ATCAGCGTCC CAGAGAATGA TTTCTTCTTT 
GACTTTGTCC GACACCTGAC AGACTGGATA AAGAAAGCAC GGCCCATCAA GGACGGAATC 
GTGCCCTCAC TAACCTACCA GGTGTTCTTC ATGAAGAAGC TGTGGACCAC CACAGTGCCG 
GGCAAGGACC CCATGGCTGA CTCCATCTTC CACTATTACC AGGAACTGCC CAAATATCTC 
CGAGGCTACC ACAAGTGCAC CCGGGAGGAG GTGCTGCAGC TGGGCGCACT CATCTACAGG 
GTCAAGTTTG AGGAGGACAA ATCCTACTTC CCTAGCATCC CCAAGTTGCT GAGGGAGCTG 
GTACCCCAGG ACCTAATCCG GCAGGTCTCA CCTGATGACT GGAAACGGTC TATTGTCGCC 
TACTTCAACA AACATGCGGG GAAGTCCAAG GAGGAAGCCA AGCTGGCCTT CCTCAAACTC 
ATCTTCAAGT GGCCCACCTT TGGCTCAGCC TTCTTTGAGG TGAAGCAAAC TACAGAACCA 
AACTTCCCAG AGATTCTCTT AATTGCCATC AACAAGTACG GGGTCAGCCT CATCGATCCC 
AGAACCAAGG ACATCCTGAC TACTCACCCC TTCACCAAGA TCTCCAACTG GAGTAGTGGC 
AACACCTACT TCCACATCAC CATTGGGAAC TTGGTCCGTG GGAGCAAACT GCTCTGTGAG 
ACATCGCTGG GATACAAAAT GGATGATCTT CTGACTTCCT ACATCAGCCA GATGCTCACA 
GCCATGAGCA AGCAGAGGAA CTCCAGGAGT GGAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241691.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239993.1 Mus musculus myosin VIIA (Myo7a), transcript variant X4, mRNA.

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CloneID	OMu20201
Clone ID Related Accession (Same CDS sequence)	XM_011241691.2 , NM_001256083.1 , XM_011241691.1
Accession Version	XM_011241691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGGAA CAAAGATGAT GGCCCCCAGC 
TTTACCCTGG CCACCATCAA AGGAGATGAG TACACCTTCA CATCCAGCAA TGCTGAGGAC 
ATCCGTGACC TGGTGGTCAC CTTTCTGGAG GGGCTACGGA AGAGGTCTAA GTATGTGGTG 
GCACTGCAGG ACAATCCTAA CCCTGCTGGT GAGGAGTCAG GCTTCCTCAG CTTCGCCAAG 
GGAGACCTCA TCATCCTTGA CCATGATACT GGTGAGCAGG TCATGAACTC AGGCTGGGCC 
AACGGCATCA ACGAGAGGAC CAAGCAGCGC GGCGACTTCC CCACTGACTG TGTATACGTC 
ATGCCCACTG TCACCTTGCC ACCAAGGGAG ATTGTGGCCC TGGTCACTAT GACCCCAGAC 
CAGAGGCAGG ATGTCGTCCG GCTCCTGCAG CTTCGCACAG CAGAGCCAGA GGTGCGCGCC 
AAGCCCTACA CGCTAGAGGA GTTCTCCTAC GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGC GGTAAGGACA GGCTGTGGAG CCACACACGA 
GAGCCCCTCA AGCAGGCCCT GCTCAAGAAG ATCCTGGGCA GTGAAGAACT CTCCCAGGAA 
GCCTGCATGG CCTTTGTAGC TGTGCTCAAG TACATGGGCG ACTACCCATC CAAGAGGATG 
CGATCCGTCA ATGAGCTCAC TGACCAGATC TTTGAGTGGG CACTCAAGGC TGAGCCCCTC 
AAGGATGAGG CCTACGTGCA GATCCTGAAG CAGCTGACTG ACAATCACAT CAGGTACAGC 
GAAGAGAGGG GCTGGGAACT GCTGTGGCTG TGCACGGGCC TCTTCCCGCC CAGCAACATC 
CTCCTGCCTC ATGTTCAGCG GTTTCTGCAG TCCCGCAAGC ACTGTCCTCT TGCCATTGAC 
TGCCTGCAGA GGCTCCAGAA AGCCCTGAGA AATGGCTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA ACATAAGACT ACCCAGATCT TCCACAAGGT CTACTTCCCC 
GATGACACGG ACGAGGCTTT TGAGGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA GCCGGCTGCT GCTCAAGTCT TCCGAGGGAT TCAGCCTTTT TGTCAAAATC 
GCAGATAAGG TCATCAGCGT CCCAGAGAAT GATTTCTTCT TTGACTTTGT CCGACACCTG 
ACAGACTGGA TAAAGAAAGC ACGGCCCATC AAGGACGGAA TCGTGCCCTC ACTAACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACAGTGC CGGGCAAGGA CCCCATGGCT 
GACTCCATCT TCCACTATTA CCAGGAACTG CCCAAATATC TCCGAGGCTA CCACAAGTGC 
ACCCGGGAGG AGGTGCTGCA GCTGGGCGCA CTCATCTACA GGGTCAAGTT TGAGGAGGAC 
AAATCCTACT TCCCTAGCAT CCCCAAGTTG CTGAGGGAGC TGGTACCCCA GGACCTAATC 
CGGCAGGTCT CACCTGATGA CTGGAAACGG TCTATTGTCG CCTACTTCAA CAAACATGCG 
GGGAAGTCCA AGGAGGAAGC CAAGCTGGCC TTCCTCAAAC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTTGA GGTGAAGCAA ACTACAGAAC CAAACTTCCC AGAGATTCTC 
TTAATTGCCA TCAACAAGTA CGGGGTCAGC CTCATCGATC CCAGAACCAA GGACATCCTG 
ACTACTCACC CCTTCACCAA GATCTCCAAC TGGAGTAGTG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTCCG TGGGAGCAAA CTGCTCTGTG AGACATCGCT GGGATACAAA 
ATGGATGATC TTCTGACTTC CTACATCAGC CAGATGCTCA CAGCCATGAG CAAGCAGAGG 
AACTCCAGGA GTGGAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239993.1
CDS	34..6534
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241691.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241691.2

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGGAA CAAAGATGAT GGCCCCCAGC 
TTTACCCTGG CCACCATCAA AGGAGATGAG TACACCTTCA CATCCAGCAA TGCTGAGGAC 
ATCCGTGACC TGGTGGTCAC CTTTCTGGAG GGGCTACGGA AGAGGTCTAA GTATGTGGTG 
GCACTGCAGG ACAATCCTAA CCCTGCTGGT GAGGAGTCAG GCTTCCTCAG CTTCGCCAAG 
GGAGACCTCA TCATCCTTGA CCATGATACT GGTGAGCAGG TCATGAACTC AGGCTGGGCC 
AACGGCATCA ACGAGAGGAC CAAGCAGCGC GGCGACTTCC CCACTGACTG TGTATACGTC 
ATGCCCACTG TCACCTTGCC ACCAAGGGAG ATTGTGGCCC TGGTCACTAT GACCCCAGAC 
CAGAGGCAGG ATGTCGTCCG GCTCCTGCAG CTTCGCACAG CAGAGCCAGA GGTGCGCGCC 
AAGCCCTACA CGCTAGAGGA GTTCTCCTAC GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGC GGTAAGGACA GGCTGTGGAG CCACACACGA 
GAGCCCCTCA AGCAGGCCCT GCTCAAGAAG ATCCTGGGCA GTGAAGAACT CTCCCAGGAA 
GCCTGCATGG CCTTTGTAGC TGTGCTCAAG TACATGGGCG ACTACCCATC CAAGAGGATG 
CGATCCGTCA ATGAGCTCAC TGACCAGATC TTTGAGTGGG CACTCAAGGC TGAGCCCCTC 
AAGGATGAGG CCTACGTGCA GATCCTGAAG CAGCTGACTG ACAATCACAT CAGGTACAGC 
GAAGAGAGGG GCTGGGAACT GCTGTGGCTG TGCACGGGCC TCTTCCCGCC CAGCAACATC 
CTCCTGCCTC ATGTTCAGCG GTTTCTGCAG TCCCGCAAGC ACTGTCCTCT TGCCATTGAC 
TGCCTGCAGA GGCTCCAGAA AGCCCTGAGA AATGGCTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA ACATAAGACT ACCCAGATCT TCCACAAGGT CTACTTCCCC 
GATGACACGG ACGAGGCTTT TGAGGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA GCCGGCTGCT GCTCAAGTCT TCCGAGGGAT TCAGCCTTTT TGTCAAAATC 
GCAGATAAGG TCATCAGCGT CCCAGAGAAT GATTTCTTCT TTGACTTTGT CCGACACCTG 
ACAGACTGGA TAAAGAAAGC ACGGCCCATC AAGGACGGAA TCGTGCCCTC ACTAACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACAGTGC CGGGCAAGGA CCCCATGGCT 
GACTCCATCT TCCACTATTA CCAGGAACTG CCCAAATATC TCCGAGGCTA CCACAAGTGC 
ACCCGGGAGG AGGTGCTGCA GCTGGGCGCA CTCATCTACA GGGTCAAGTT TGAGGAGGAC 
AAATCCTACT TCCCTAGCAT CCCCAAGTTG CTGAGGGAGC TGGTACCCCA GGACCTAATC 
CGGCAGGTCT CACCTGATGA CTGGAAACGG TCTATTGTCG CCTACTTCAA CAAACATGCG 
GGGAAGTCCA AGGAGGAAGC CAAGCTGGCC TTCCTCAAAC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTTGA GGTGAAGCAA ACTACAGAAC CAAACTTCCC AGAGATTCTC 
TTAATTGCCA TCAACAAGTA CGGGGTCAGC CTCATCGATC CCAGAACCAA GGACATCCTG 
ACTACTCACC CCTTCACCAA GATCTCCAAC TGGAGTAGTG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTCCG TGGGAGCAAA CTGCTCTGTG AGACATCGCT GGGATACAAA 
ATGGATGATC TTCTGACTTC CTACATCAGC CAGATGCTCA CAGCCATGAG CAAGCAGAGG 
AACTCCAGGA GTGGAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_006507427.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507490.1 Mus musculus myosin VIIA (Myo7a), transcript variant X5, mRNA.

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CloneID	OMu39357
Clone ID Related Accession (Same CDS sequence)	XM_006507427.4 , XM_006507427.3
Accession Version	XM_006507427.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507427.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACCAG CTGTGGAGGA 
CGGACGTACA CAGGTTCCAT CCTGGTGGCC GTGAACCCCT ACCAGCTGCT CTCCATCTAC 
TCGCCAGAGC ACATCCGCCA GTACACCAAC AAGAAGATAG GGGAGATGCC CCCCCACATC 
TTCGCCATTG CTGACAACTG CTACTTCAAC ATGAAACGCA ACAACCGGGA CCAGTGCTGT 
ATTATCAGCG GGGAGTCGGG AGCTGGCAAG ACAGAGAGCA CAAAGTTGAT CCTGCAGTTC 
CTGGCAGCCA TCAGTGGACA GCACTCATGG ATCGAGCAGC AGGTGCTGGA GGCCACCCCG 
ATCCTGGAAG CATTTGGGAA CGCCAAGACC ATCCGCAACG ACAACTCTAG CCGCTTTGGC 
AAGTACATTG ACATCCACTT TAACAAGCGT GGTGCCATCG AGGGCGCCAA AATAGAGCAA 
TACCTGCTGG AGAAGTCACG TGTCTGCCGC CAGGCCCCTG ACGAGAGGAA CTATCACGTG 
TTCTACTGTA TGCTGGAGGG CATGAATGAG GAGGAGAAGA AGAAACTGGG CCTAGGCCAG 
GCCGCTGACT ACAACTACTT GGCCATGGGT AACTGCATCA CCTGTGAGGG CCGCGTGGAC 
AGTCAGGAGT ATGCCAACAT CCGCTCTGCC ATGAAGGTTC TCATGTTCAC AGACACGGAG 
AACTGGGAGA TCTCGAAGCT TCTGGCTGCC ATCCTACACA TGGGCAATCT GCAGTATGAG 
GCCCGGACAT TTGAGAACTT GGATGCGTGT GAAGTCCTCT TCTCCCCATC GCTGGCCACG 
GCAGCTTCTC TGCTCGAGGT GAACCCCCCA GACCTGATGA GCTGCCTCAC CAGCCGCACC 
CTCATCACCC GTGGGGAGAC GGTGTCCACC CCTCTCAGCA GGGAACAGGC GCTGGATGTG 
CGAGATGCCT TTGTCAAGGG CATCTATGGG CGGCTCTTTG TGTGGATTGT GGAGAAGATC 
AACGCAGCAA TCTACAAGCC ACCCCCCCTG GAAGTGAAGA ACTCTCGCCG GTCCATCGGT 
CTCCTGGACA TCTTTGGATT TGAGAACTTC ACTGTGAACA GCTTCGAGCA GCTCTGCATT 
AACTTTGCCA ATGAGCACCT GCAGCAATTC TTCGTGCGGC ACGTGTTCAA GCTGGAGCAG 
GAGGAGTACG ACCTGGAGAG CATCGACTGG TTGCACATTG AGTTCACTGA CAACCAGGAA 
GCACTGGACA TGATTGCCAA CCGGCCTATG AACGTCATCT CCCTCATCGA TGAGGAGAGC 
AAGTTCCCCA AGGGCACGGA TGCCACCATG CTGCATAAGC TGAACTCACA GCACAAGCTC 
AATGCCAACT ACGTGCCACC CAAGAACAGC CACGAGACCC AGTTTGGAAT CAACCACTTT 
GCGGGTGTTG TCTATTATGA GAGTCAAGGC TTCCTGGAGA AGAACCGAGA CACCCTGCAT 
GGGGACATCA TCCAGCTGGT CCACTCTTCC CGGAACAAGT TCATAAAGCA GATTTTCCAA 
GCTGACGTTG CCATGGGTGC CGAGACCAGG AAGCGCTCGC CTACACTCAG CAGCCAGTTC 
AAGCGGTCTC TGGAGCTGCT GATGCGCACA CTGGGCGCCT GCCAGCCCTT CTTTGTGCGT 
TGTATCAAAC CCAATGAGTT CAAGAAGCCC ATGCTCTTCG ACCGGCACTT GTGTGTACGC 
CAGCTGCGAT ATTCGGGCAT GATGGAGACA ATCCGCATCC GCCACGCAGG CTACCCCATT 
CGCTACAGCT TTGTGGAGTT TGTGGAGCGC TACCGGGTAC TGCTGCCTGG TGTGAAGCCA 
GCATACAAGC AGGGTGACCT CCGAGGGACA TGCCAGCGCA TGGCTGAGGC TGTGCTGGGC 
ACGCACGATG ACTGGCAGAT TGGCAAAACC AAGATCTTTC TGAAGGACCA CCATGACATG 
TTGCTGGAGG TGGAGCGGGA CAAGGCCATC ACAGACAGAG TCATTCTCCT CCAGAAGGTT 
ATCCGGGGCT TCAAAGACAG GTCCAACTTC CTGAGACTGA AGAGTGCTGC CACACTGATC 
CAGAGGCACT GGCGGGGCCA CCACTGTAGG AAAAACTATG AGCTGATTCG TCTTGGCTTC 
CTGCGGCTGC AGGCCCTGCA CCGCTCCCGG AAGCTGCACA AGCAGTACCG CCTGGCCAGA 
CAGCGCATAA TAGAGTTCCA GGCCCGCTGC CGGGCCTATC TGGTGCGCAA GGCCTTCCGC 
CACCGCCTCT GGGCCGTGAT CACCGTGCAG GCCTATGCCC GAGGCATGAT TGCCCGCCGG 
CTACACCGCC GCCTCCGGGT TGAGTACCAG CGGCGCCTCG AGGCAGAGAG GATGCGTCTG 
GCAGAGGAGG AGAAACTCCG AAAGGAGATG AGTGCCAAGA AGGCCAAAGA GGAGGCTGAG 
CGCAAGCATC AGGAGCGCCT GGCTCAGCTA GCCCGCGAGG ATGCGGAGCG GGAACTGAAG 
GAGAAGGAGG AGGCTCGGAG GAAGAAGGAA CTGCTGGAGC AGATGGAGAA GGCCCGCCAC 
GAACCCATCA ACCACTCAGA TATGGTGGAC AAGATGTTTG GCTTCCTGGG GACTTCAGGC 
AGCCTGCCAG GCCAGGAAGG CCAGGCGCCT AGTGGCTTTG AGGACCTAGA GCGCGGACGG 
AGGGAGATGG TGGAAGAGGA TGTTGACGCT GCCCTGCCCC TGCCTGATGA AGACGAGGAG 
GACCTTTCTG AGTACAAATT CGCCAAGTTT GCTGCCACCT ACTTCCAGGG CACAACCACA 
CACTCCTACA CCCGGAGGCC TCTCAAGCAG CCGCTGCTCT ACCACGACGA TGAGGGTGAC 
CAGCTGGCGG CGCTGGCTGT CTGGATCACC ATCCTCCGGT TCATGGGGGA CCTCCCAGAG 
CCCAAGTACC ACACAGCCAT GAGCGACGGC AGTGAGAAGA TCCCAGTGAT GACTAAGATC 
TACGAGACCC TAGGCAAGAA GACATATAAG AGGGAGCTGC AGGCCTTGCA GGGCGAGGGC 
GAGACCCAGC TCCCTGAGGG GCAGAAGAAG ACCAGTGTGA GACACAAGTT GGTACACTTG 
ACACTGAAGA AAAAGTCCAA ACTCACAGAA GAGGTGACCA AGAGGCTGAA CGATGGGGAA 
TCCACGGTAC AGGGCAACAG CATGCTGGAG GATCGGCCCA CCTCAAATCT AGAGAAGCTG 
CACTTCATCA TCGGCAACGG CATCCTGCGG CCTGCGCTGC GGGACGAGAT TTACTGCCAG 
ATCAGTAAGC AGCTCACACA CAACCCATCC AAGAGCAGCT ATGCCAGGGG CTGGATCCTC 
GTGTCGCTCT GTGTGGGCTG CTTCGCCCCC TCTGAGAAGT TCGTTAAGTA CCTGCGGAAC 
TTCATCCACG GAGGCCCACC TGGCTATGCT CCTTACTGTG AGGAGCGCCT GAGGAGGACC 
TTTGTCAACG GAACTCGGAC ACAGCCACCC AGCTGGCTGG AGCTGCAGGC CACCAAGTCC 
AAGAAGCCCA TCATGTTGCC CGTGACCTTC ATGGATGGGA CCACCAAGAC CCTGCTAACA 
GATTCAGCAA CTACAGCCAG GGAGCTGTGC AATGCTCTGG CTGACAAGAT CTCACTCAAG 
GACCGCTTTG GCTTCTCCCT CTACATCGCT CTGTTCGATA AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ATGTCATGGA TGCCATCTCT CAGTGTGAGC AGTACGCCAA GGAGCAGGGT 
GCTCAGGAGC GCAACGCCCC ATGGAGGCTC TTCTTTAGAA AGGAGGTCTT CACACCCTGG 
CACAACCCCT CGGAGGACAA CGTGGCCACG AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCTTCTCAG 
CAGTACTTTG TGGACTATGG TTCTGAGATG ATTCTGGAGC GCCTGCTGAG CCTCGTGCCC 
ACTTACATCC CTGACCGTGA GATCACACCG CTGAAGAATC TTGAGAAGTG GGCACAGCTG 
GCCATTGCTG CCCACAAGAA GGGAATTTAT GCCCAGAGGA GAACTGACTC CCAGAAGGTC 
AAAGAGGATG TGGTCAATTA TGCCCGTTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAC 
GAAGCTTACA AATTCTCAGG CCCTCCCCTC CCCAAGAGCG ACGTCATCGT GGCTGTCAAC 
TGGACGGGTG TGTACTTCGT GGACGAGCAG GAGCAGGTGC TTCTGGAGCT GTCCTTCCCG 
GAGATCATGG CTGTGTCCAG CAGTAGGGAG TGCCGCGTCT TGCTCTCACT GGGCTGCTCT 
GACTTGGGCT GTGCTACTTG TCAATCGGGC CGGGCAGGGC TGACCCCGGC TGGACCCTGT 
TCTCCGTGTT GGTCCTGTAG GGGAACAAAG ATGATGGCCC CCAGCTTTAC CCTGGCCACC 
ATCAAAGGAG ATGAGTACAC CTTCACATCC AGCAATGCTG AGGACATCCG TGACCTGGTG 
GTCACCTTTC TGGAGGGGCT ACGGAAGAGG TCTAAGTATG TGGTGGCACT GCAGGACAAT 
CCTAACCCTG CTGGTGAGGA GTCAGGCTTC CTCAGCTTCG CCAAGGGAGA CCTCATCATC 
CTTGACCATG ATACTGGTGA GCAGGTCATG AACTCAGGCT GGGCCAACGG CATCAACGAG 
AGGACCAAGC AGCGCGGCGA CTTCCCCACT GACTGTGTAT ACGTCATGCC CACTGTCACC 
TTGCCACCAA GGGAGATTGT GGCCCTGGTC ACTATGACCC CAGACCAGAG GCAGGATGTC 
GTCCGGCTCC TGCAGCTTCG CACAGCAGAG CCAGAGGTGC GCGCCAAGCC CTACACGCTA 
GAGGAGTTCT CCTACGACTA CTTCAGGCCC CCACCCAAGC ACACGCTGAG CCGTGTCATG 
GTGTCCAAGG CCCGCGGTAA GGACAGGCTG TGGAGCCACA CACGAGAGCC CCTCAAGCAG 
GCCCTGCTCA AGAAGATCCT GGGCAGTGAA GAACTCTCCC AGGAAGCCTG CATGGCCTTT 
GTAGCTGTGC TCAAGTACAT GGGCGACTAC CCATCCAAGA GGATGCGATC CGTCAATGAG 
CTCACTGACC AGATCTTTGA GTGGGCACTC AAGGCTGAGC CCCTCAAGGA TGAGGCCTAC 
GTGCAGATCC TGAAGCAGCT GACTGACAAT CACATCAGGT ACAGCGAAGA GAGGGGCTGG 
GAACTGCTGT GGCTGTGCAC GGGCCTCTTC CCGCCCAGCA ACATCCTCCT GCCTCATGTT 
CAGCGGTTTC TGCAGTCCCG CAAGCACTGT CCTCTTGCCA TTGACTGCCT GCAGAGGCTC 
CAGAAAGCCC TGAGAAATGG CTCCCGGAAG TACCCTCCGC ACCTGGTGGA GGTGGAGGCC 
ATCCAACATA AGACTACCCA GATCTTCCAC AAGGTCTACT TCCCCGATGA CACGGACGAG 
GCTTTTGAGG TGGAGTCCAG CACCAAGGCC AAGGACTTCT GCCAGAACAT CGCCAGCCGG 
CTGCTGCTCA AGTCTTCCGA GGGATTCAGC CTTTTTGTCA AAATCGCAGA TAAGGTCATC 
AGCGTCCCAG AGAATGATTT CTTCTTTGAC TTTGTCCGAC ACCTGACAGA CTGGATAAAG 
AAAGCACGGC CCATCAAGGA CGGAATCGTG CCCTCACTAA CCTACCAGGT GTTCTTCATG 
AAGAAGCTGT GGACCACCAC AGTGCCGGGC AAGGACCCCA TGGCTGACTC CATCTTCCAC 
TATTACCAGG AACTGCCCAA ATATCTCCGA GGCTACCACA AGTGCACCCG GGAGGAGGTG 
CTGCAGCTGG GCGCACTCAT CTACAGGGTC AAGTTTGAGG AGGACAAATC CTACTTCCCT 
AGCATCCCCA AGTTGCTGAG GGAGCTGGTA CCCCAGGACC TAATCCGGCA GGTCTCACCT 
GATGACTGGA AACGGTCTAT TGTCGCCTAC TTCAACAAAC ATGCGGGGAA GTCCAAGGAG 
GAAGCCAAGC TGGCCTTCCT CAAACTCATC TTCAAGTGGC CCACCTTTGG CTCAGCCTTC 
TTTGAGGTGA AGCAAACTAC AGAACCAAAC TTCCCAGAGA TTCTCTTAAT TGCCATCAAC 
AAGTACGGGG TCAGCCTCAT CGATCCCAGA ACCAAGGACA TCCTGACTAC TCACCCCTTC 
ACCAAGATCT CCAACTGGAG TAGTGGCAAC ACCTACTTCC ACATCACCAT TGGGAACTTG 
GTCCGTGGGA GCAAACTGCT CTGTGAGACA TCGCTGGGAT ACAAAATGGA TGATCTTCTG 
ACTTCCTACA TCAGCCAGAT GCTCACAGCC ATGAGCAAGC AGAGGAACTC CAGGAGTGGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507490.1
CDS	290..6955
Translation	MVILQKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_006507427.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507427.4

ORF Insert Sequence:

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACCAG CTGTGGAGGA 
CGGACGTACA CAGGTTCCAT CCTGGTGGCC GTGAACCCCT ACCAGCTGCT CTCCATCTAC 
TCGCCAGAGC ACATCCGCCA GTACACCAAC AAGAAGATAG GGGAGATGCC CCCCCACATC 
TTCGCCATTG CTGACAACTG CTACTTCAAC ATGAAACGCA ACAACCGGGA CCAGTGCTGT 
ATTATCAGCG GGGAGTCGGG AGCTGGCAAG ACAGAGAGCA CAAAGTTGAT CCTGCAGTTC 
CTGGCAGCCA TCAGTGGACA GCACTCATGG ATCGAGCAGC AGGTGCTGGA GGCCACCCCG 
ATCCTGGAAG CATTTGGGAA CGCCAAGACC ATCCGCAACG ACAACTCTAG CCGCTTTGGC 
AAGTACATTG ACATCCACTT TAACAAGCGT GGTGCCATCG AGGGCGCCAA AATAGAGCAA 
TACCTGCTGG AGAAGTCACG TGTCTGCCGC CAGGCCCCTG ACGAGAGGAA CTATCACGTG 
TTCTACTGTA TGCTGGAGGG CATGAATGAG GAGGAGAAGA AGAAACTGGG CCTAGGCCAG 
GCCGCTGACT ACAACTACTT GGCCATGGGT AACTGCATCA CCTGTGAGGG CCGCGTGGAC 
AGTCAGGAGT ATGCCAACAT CCGCTCTGCC ATGAAGGTTC TCATGTTCAC AGACACGGAG 
AACTGGGAGA TCTCGAAGCT TCTGGCTGCC ATCCTACACA TGGGCAATCT GCAGTATGAG 
GCCCGGACAT TTGAGAACTT GGATGCGTGT GAAGTCCTCT TCTCCCCATC GCTGGCCACG 
GCAGCTTCTC TGCTCGAGGT GAACCCCCCA GACCTGATGA GCTGCCTCAC CAGCCGCACC 
CTCATCACCC GTGGGGAGAC GGTGTCCACC CCTCTCAGCA GGGAACAGGC GCTGGATGTG 
CGAGATGCCT TTGTCAAGGG CATCTATGGG CGGCTCTTTG TGTGGATTGT GGAGAAGATC 
AACGCAGCAA TCTACAAGCC ACCCCCCCTG GAAGTGAAGA ACTCTCGCCG GTCCATCGGT 
CTCCTGGACA TCTTTGGATT TGAGAACTTC ACTGTGAACA GCTTCGAGCA GCTCTGCATT 
AACTTTGCCA ATGAGCACCT GCAGCAATTC TTCGTGCGGC ACGTGTTCAA GCTGGAGCAG 
GAGGAGTACG ACCTGGAGAG CATCGACTGG TTGCACATTG AGTTCACTGA CAACCAGGAA 
GCACTGGACA TGATTGCCAA CCGGCCTATG AACGTCATCT CCCTCATCGA TGAGGAGAGC 
AAGTTCCCCA AGGGCACGGA TGCCACCATG CTGCATAAGC TGAACTCACA GCACAAGCTC 
AATGCCAACT ACGTGCCACC CAAGAACAGC CACGAGACCC AGTTTGGAAT CAACCACTTT 
GCGGGTGTTG TCTATTATGA GAGTCAAGGC TTCCTGGAGA AGAACCGAGA CACCCTGCAT 
GGGGACATCA TCCAGCTGGT CCACTCTTCC CGGAACAAGT TCATAAAGCA GATTTTCCAA 
GCTGACGTTG CCATGGGTGC CGAGACCAGG AAGCGCTCGC CTACACTCAG CAGCCAGTTC 
AAGCGGTCTC TGGAGCTGCT GATGCGCACA CTGGGCGCCT GCCAGCCCTT CTTTGTGCGT 
TGTATCAAAC CCAATGAGTT CAAGAAGCCC ATGCTCTTCG ACCGGCACTT GTGTGTACGC 
CAGCTGCGAT ATTCGGGCAT GATGGAGACA ATCCGCATCC GCCACGCAGG CTACCCCATT 
CGCTACAGCT TTGTGGAGTT TGTGGAGCGC TACCGGGTAC TGCTGCCTGG TGTGAAGCCA 
GCATACAAGC AGGGTGACCT CCGAGGGACA TGCCAGCGCA TGGCTGAGGC TGTGCTGGGC 
ACGCACGATG ACTGGCAGAT TGGCAAAACC AAGATCTTTC TGAAGGACCA CCATGACATG 
TTGCTGGAGG TGGAGCGGGA CAAGGCCATC ACAGACAGAG TCATTCTCCT CCAGAAGGTT 
ATCCGGGGCT TCAAAGACAG GTCCAACTTC CTGAGACTGA AGAGTGCTGC CACACTGATC 
CAGAGGCACT GGCGGGGCCA CCACTGTAGG AAAAACTATG AGCTGATTCG TCTTGGCTTC 
CTGCGGCTGC AGGCCCTGCA CCGCTCCCGG AAGCTGCACA AGCAGTACCG CCTGGCCAGA 
CAGCGCATAA TAGAGTTCCA GGCCCGCTGC CGGGCCTATC TGGTGCGCAA GGCCTTCCGC 
CACCGCCTCT GGGCCGTGAT CACCGTGCAG GCCTATGCCC GAGGCATGAT TGCCCGCCGG 
CTACACCGCC GCCTCCGGGT TGAGTACCAG CGGCGCCTCG AGGCAGAGAG GATGCGTCTG 
GCAGAGGAGG AGAAACTCCG AAAGGAGATG AGTGCCAAGA AGGCCAAAGA GGAGGCTGAG 
CGCAAGCATC AGGAGCGCCT GGCTCAGCTA GCCCGCGAGG ATGCGGAGCG GGAACTGAAG 
GAGAAGGAGG AGGCTCGGAG GAAGAAGGAA CTGCTGGAGC AGATGGAGAA GGCCCGCCAC 
GAACCCATCA ACCACTCAGA TATGGTGGAC AAGATGTTTG GCTTCCTGGG GACTTCAGGC 
AGCCTGCCAG GCCAGGAAGG CCAGGCGCCT AGTGGCTTTG AGGACCTAGA GCGCGGACGG 
AGGGAGATGG TGGAAGAGGA TGTTGACGCT GCCCTGCCCC TGCCTGATGA AGACGAGGAG 
GACCTTTCTG AGTACAAATT CGCCAAGTTT GCTGCCACCT ACTTCCAGGG CACAACCACA 
CACTCCTACA CCCGGAGGCC TCTCAAGCAG CCGCTGCTCT ACCACGACGA TGAGGGTGAC 
CAGCTGGCGG CGCTGGCTGT CTGGATCACC ATCCTCCGGT TCATGGGGGA CCTCCCAGAG 
CCCAAGTACC ACACAGCCAT GAGCGACGGC AGTGAGAAGA TCCCAGTGAT GACTAAGATC 
TACGAGACCC TAGGCAAGAA GACATATAAG AGGGAGCTGC AGGCCTTGCA GGGCGAGGGC 
GAGACCCAGC TCCCTGAGGG GCAGAAGAAG ACCAGTGTGA GACACAAGTT GGTACACTTG 
ACACTGAAGA AAAAGTCCAA ACTCACAGAA GAGGTGACCA AGAGGCTGAA CGATGGGGAA 
TCCACGGTAC AGGGCAACAG CATGCTGGAG GATCGGCCCA CCTCAAATCT AGAGAAGCTG 
CACTTCATCA TCGGCAACGG CATCCTGCGG CCTGCGCTGC GGGACGAGAT TTACTGCCAG 
ATCAGTAAGC AGCTCACACA CAACCCATCC AAGAGCAGCT ATGCCAGGGG CTGGATCCTC 
GTGTCGCTCT GTGTGGGCTG CTTCGCCCCC TCTGAGAAGT TCGTTAAGTA CCTGCGGAAC 
TTCATCCACG GAGGCCCACC TGGCTATGCT CCTTACTGTG AGGAGCGCCT GAGGAGGACC 
TTTGTCAACG GAACTCGGAC ACAGCCACCC AGCTGGCTGG AGCTGCAGGC CACCAAGTCC 
AAGAAGCCCA TCATGTTGCC CGTGACCTTC ATGGATGGGA CCACCAAGAC CCTGCTAACA 
GATTCAGCAA CTACAGCCAG GGAGCTGTGC AATGCTCTGG CTGACAAGAT CTCACTCAAG 
GACCGCTTTG GCTTCTCCCT CTACATCGCT CTGTTCGATA AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ATGTCATGGA TGCCATCTCT CAGTGTGAGC AGTACGCCAA GGAGCAGGGT 
GCTCAGGAGC GCAACGCCCC ATGGAGGCTC TTCTTTAGAA AGGAGGTCTT CACACCCTGG 
CACAACCCCT CGGAGGACAA CGTGGCCACG AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCTTCTCAG 
CAGTACTTTG TGGACTATGG TTCTGAGATG ATTCTGGAGC GCCTGCTGAG CCTCGTGCCC 
ACTTACATCC CTGACCGTGA GATCACACCG CTGAAGAATC TTGAGAAGTG GGCACAGCTG 
GCCATTGCTG CCCACAAGAA GGGAATTTAT GCCCAGAGGA GAACTGACTC CCAGAAGGTC 
AAAGAGGATG TGGTCAATTA TGCCCGTTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAC 
GAAGCTTACA AATTCTCAGG CCCTCCCCTC CCCAAGAGCG ACGTCATCGT GGCTGTCAAC 
TGGACGGGTG TGTACTTCGT GGACGAGCAG GAGCAGGTGC TTCTGGAGCT GTCCTTCCCG 
GAGATCATGG CTGTGTCCAG CAGTAGGGAG TGCCGCGTCT TGCTCTCACT GGGCTGCTCT 
GACTTGGGCT GTGCTACTTG TCAATCGGGC CGGGCAGGGC TGACCCCGGC TGGACCCTGT 
TCTCCGTGTT GGTCCTGTAG GGGAACAAAG ATGATGGCCC CCAGCTTTAC CCTGGCCACC 
ATCAAAGGAG ATGAGTACAC CTTCACATCC AGCAATGCTG AGGACATCCG TGACCTGGTG 
GTCACCTTTC TGGAGGGGCT ACGGAAGAGG TCTAAGTATG TGGTGGCACT GCAGGACAAT 
CCTAACCCTG CTGGTGAGGA GTCAGGCTTC CTCAGCTTCG CCAAGGGAGA CCTCATCATC 
CTTGACCATG ATACTGGTGA GCAGGTCATG AACTCAGGCT GGGCCAACGG CATCAACGAG 
AGGACCAAGC AGCGCGGCGA CTTCCCCACT GACTGTGTAT ACGTCATGCC CACTGTCACC 
TTGCCACCAA GGGAGATTGT GGCCCTGGTC ACTATGACCC CAGACCAGAG GCAGGATGTC 
GTCCGGCTCC TGCAGCTTCG CACAGCAGAG CCAGAGGTGC GCGCCAAGCC CTACACGCTA 
GAGGAGTTCT CCTACGACTA CTTCAGGCCC CCACCCAAGC ACACGCTGAG CCGTGTCATG 
GTGTCCAAGG CCCGCGGTAA GGACAGGCTG TGGAGCCACA CACGAGAGCC CCTCAAGCAG 
GCCCTGCTCA AGAAGATCCT GGGCAGTGAA GAACTCTCCC AGGAAGCCTG CATGGCCTTT 
GTAGCTGTGC TCAAGTACAT GGGCGACTAC CCATCCAAGA GGATGCGATC CGTCAATGAG 
CTCACTGACC AGATCTTTGA GTGGGCACTC AAGGCTGAGC CCCTCAAGGA TGAGGCCTAC 
GTGCAGATCC TGAAGCAGCT GACTGACAAT CACATCAGGT ACAGCGAAGA GAGGGGCTGG 
GAACTGCTGT GGCTGTGCAC GGGCCTCTTC CCGCCCAGCA ACATCCTCCT GCCTCATGTT 
CAGCGGTTTC TGCAGTCCCG CAAGCACTGT CCTCTTGCCA TTGACTGCCT GCAGAGGCTC 
CAGAAAGCCC TGAGAAATGG CTCCCGGAAG TACCCTCCGC ACCTGGTGGA GGTGGAGGCC 
ATCCAACATA AGACTACCCA GATCTTCCAC AAGGTCTACT TCCCCGATGA CACGGACGAG 
GCTTTTGAGG TGGAGTCCAG CACCAAGGCC AAGGACTTCT GCCAGAACAT CGCCAGCCGG 
CTGCTGCTCA AGTCTTCCGA GGGATTCAGC CTTTTTGTCA AAATCGCAGA TAAGGTCATC 
AGCGTCCCAG AGAATGATTT CTTCTTTGAC TTTGTCCGAC ACCTGACAGA CTGGATAAAG 
AAAGCACGGC CCATCAAGGA CGGAATCGTG CCCTCACTAA CCTACCAGGT GTTCTTCATG 
AAGAAGCTGT GGACCACCAC AGTGCCGGGC AAGGACCCCA TGGCTGACTC CATCTTCCAC 
TATTACCAGG AACTGCCCAA ATATCTCCGA GGCTACCACA AGTGCACCCG GGAGGAGGTG 
CTGCAGCTGG GCGCACTCAT CTACAGGGTC AAGTTTGAGG AGGACAAATC CTACTTCCCT 
AGCATCCCCA AGTTGCTGAG GGAGCTGGTA CCCCAGGACC TAATCCGGCA GGTCTCACCT 
GATGACTGGA AACGGTCTAT TGTCGCCTAC TTCAACAAAC ATGCGGGGAA GTCCAAGGAG 
GAAGCCAAGC TGGCCTTCCT CAAACTCATC TTCAAGTGGC CCACCTTTGG CTCAGCCTTC 
TTTGAGGTGA AGCAAACTAC AGAACCAAAC TTCCCAGAGA TTCTCTTAAT TGCCATCAAC 
AAGTACGGGG TCAGCCTCAT CGATCCCAGA ACCAAGGACA TCCTGACTAC TCACCCCTTC 
ACCAAGATCT CCAACTGGAG TAGTGGCAAC ACCTACTTCC ACATCACCAT TGGGAACTTG 
GTCCGTGGGA GCAAACTGCT CTGTGAGACA TCGCTGGGAT ACAAAATGGA TGATCTTCTG 
ACTTCCTACA TCAGCCAGAT GCTCACAGCC ATGAGCAAGC AGAGGAACTC CAGGAGTGGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_006507428.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507491.1 Mus musculus myosin VIIA (Myo7a), transcript variant X6, mRNA.

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CloneID	OMu39358
Clone ID Related Accession (Same CDS sequence)	XM_006507428.2 , XM_006507428.3
Accession Version	XM_006507428.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507428.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACCAGCTGT 
GGAGGACGGA CGTACACAGG TTCCATCCTG GTGGCCGTGA ACCCCTACCA GCTGCTCTCC 
ATCTACTCGC CAGAGCACAT CCGCCAGTAC ACCAACAAGA AGATAGGGGA GATGCCCCCC 
CACATCTTCG CCATTGCTGA CAACTGCTAC TTCAACATGA AACGCAACAA CCGGGACCAG 
TGCTGTATTA TCAGCGGGGA GTCGGGAGCT GGCAAGACAG AGAGCACAAA GTTGATCCTG 
CAGTTCCTGG CAGCCATCAG TGGACAGCAC TCATGGATCG AGCAGCAGGT GCTGGAGGCC 
ACCCCGATCC TGGAAGCATT TGGGAACGCC AAGACCATCC GCAACGACAA CTCTAGCCGC 
TTTGGCAAGT ACATTGACAT CCACTTTAAC AAGCGTGGTG CCATCGAGGG CGCCAAAATA 
GAGCAATACC TGCTGGAGAA GTCACGTGTC TGCCGCCAGG CCCCTGACGA GAGGAACTAT 
CACGTGTTCT ACTGTATGCT GGAGGGCATG AATGAGGAGG AGAAGAAGAA ACTGGGCCTA 
GGCCAGGCCG CTGACTACAA CTACTTGGCC ATGGGTAACT GCATCACCTG TGAGGGCCGC 
GTGGACAGTC AGGAGTATGC CAACATCCGC TCTGCCATGA AGGTTCTCAT GTTCACAGAC 
ACGGAGAACT GGGAGATCTC GAAGCTTCTG GCTGCCATCC TACACATGGG CAATCTGCAG 
TATGAGGCCC GGACATTTGA GAACTTGGAT GCGTGTGAAG TCCTCTTCTC CCCATCGCTG 
GCCACGGCAG CTTCTCTGCT CGAGGTGAAC CCCCCAGACC TGATGAGCTG CCTCACCAGC 
CGCACCCTCA TCACCCGTGG GGAGACGGTG TCCACCCCTC TCAGCAGGGA ACAGGCGCTG 
GATGTGCGAG ATGCCTTTGT CAAGGGCATC TATGGGCGGC TCTTTGTGTG GATTGTGGAG 
AAGATCAACG CAGCAATCTA CAAGCCACCC CCCCTGGAAG TGAAGAACTC TCGCCGGTCC 
ATCGGTCTCC TGGACATCTT TGGATTTGAG AACTTCACTG TGAACAGCTT CGAGCAGCTC 
TGCATTAACT TTGCCAATGA GCACCTGCAG CAATTCTTCG TGCGGCACGT GTTCAAGCTG 
GAGCAGGAGG AGTACGACCT GGAGAGCATC GACTGGTTGC ACATTGAGTT CACTGACAAC 
CAGGAAGCAC TGGACATGAT TGCCAACCGG CCTATGAACG TCATCTCCCT CATCGATGAG 
GAGAGCAAGT TCCCCAAGGG CACGGATGCC ACCATGCTGC ATAAGCTGAA CTCACAGCAC 
AAGCTCAATG CCAACTACGT GCCACCCAAG AACAGCCACG AGACCCAGTT TGGAATCAAC 
CACTTTGCGG GTGTTGTCTA TTATGAGAGT CAAGGCTTCC TGGAGAAGAA CCGAGACACC 
CTGCATGGGG ACATCATCCA GCTGGTCCAC TCTTCCCGGA ACAAGTTCAT AAAGCAGATT 
TTCCAAGCTG ACGTTGCCAT GGGTGCCGAG ACCAGGAAGC GCTCGCCTAC ACTCAGCAGC 
CAGTTCAAGC GGTCTCTGGA GCTGCTGATG CGCACACTGG GCGCCTGCCA GCCCTTCTTT 
GTGCGTTGTA TCAAACCCAA TGAGTTCAAG AAGCCCATGC TCTTCGACCG GCACTTGTGT 
GTACGCCAGC TGCGATATTC GGGCATGATG GAGACAATCC GCATCCGCCA CGCAGGCTAC 
CCCATTCGCT ACAGCTTTGT GGAGTTTGTG GAGCGCTACC GGGTACTGCT GCCTGGTGTG 
AAGCCAGCAT ACAAGCAGGG TGACCTCCGA GGGACATGCC AGCGCATGGC TGAGGCTGTG 
CTGGGCACGC ACGATGACTG GCAGATTGGC AAAACCAAGA TCTTTCTGAA GGACCACCAT 
GACATGTTGC TGGAGGTGGA GCGGGACAAG GCCATCACAG ACAGAGTCAT TCTCCTCCAG 
AAGGTTATCC GGGGCTTCAA AGACAGGTCC AACTTCCTGA GACTGAAGAG TGCTGCCACA 
CTGATCCAGA GGCACTGGCG GGGCCACCAC TGTAGGAAAA ACTATGAGCT GATTCGTCTT 
GGCTTCCTGC GGCTGCAGGC CCTGCACCGC TCCCGGAAGC TGCACAAGCA GTACCGCCTG 
GCCAGACAGC GCATAATAGA GTTCCAGGCC CGCTGCCGGG CCTATCTGGT GCGCAAGGCC 
TTCCGCCACC GCCTCTGGGC CGTGATCACC GTGCAGGCCT ATGCCCGAGG CATGATTGCC 
CGCCGGCTAC ACCGCCGCCT CCGGGTTGAG TACCAGCGGC GCCTCGAGGC AGAGAGGATG 
CGTCTGGCAG AGGAGGAGAA ACTCCGAAAG GAGATGAGTG CCAAGAAGGC CAAAGAGGAG 
GCTGAGCGCA AGCATCAGGA GCGCCTGGCT CAGCTAGCCC GCGAGGATGC GGAGCGGGAA 
CTGAAGGAGA AGGAGGAGGC TCGGAGGAAG AAGGAACTGC TGGAGCAGAT GGAGAAGGCC 
CGCCACGAAC CCATCAACCA CTCAGATATG GTGGACAAGA TGTTTGGCTT CCTGGGGACT 
TCAGGCAGCC TGCCAGGCCA GGAAGGCCAG GCGCCTAGTG GCTTTGAGGA CCTAGAGCGC 
GGACGGAGGG AGATGGTGGA AGAGGATGTT GACGCTGCCC TGCCCCTGCC TGATGAAGAC 
GAGGAGGACC TTTCTGAGTA CAAATTCGCC AAGTTTGCTG CCACCTACTT CCAGGGCACA 
ACCACACACT CCTACACCCG GAGGCCTCTC AAGCAGCCGC TGCTCTACCA CGACGATGAG 
GGTGACCAGC TGGCGGCGCT GGCTGTCTGG ATCACCATCC TCCGGTTCAT GGGGGACCTC 
CCAGAGCCCA AGTACCACAC AGCCATGAGC GACGGCAGTG AGAAGATCCC AGTGATGACT 
AAGATCTACG AGACCCTAGG CAAGAAGACA TATAAGAGGG AGCTGCAGGC CTTGCAGGGC 
GAGGGCGAGA CCCAGCTCCC TGAGGGGCAG AAGAAGACCA GTGTGAGACA CAAGTTGGTA 
CACTTGACAC TGAAGAAAAA GTCCAAACTC ACAGAAGAGG TGACCAAGAG GCTGAACGAT 
GGGGAATCCA CGGTACAGGG CAACAGCATG CTGGAGGATC GGCCCACCTC AAATCTAGAG 
AAGCTGCACT TCATCATCGG CAACGGCATC CTGCGGCCTG CGCTGCGGGA CGAGATTTAC 
TGCCAGATCA GTAAGCAGCT CACACACAAC CCATCCAAGA GCAGCTATGC CAGGGGCTGG 
ATCCTCGTGT CGCTCTGTGT GGGCTGCTTC GCCCCCTCTG AGAAGTTCGT TAAGTACCTG 
CGGAACTTCA TCCACGGAGG CCCACCTGGC TATGCTCCTT ACTGTGAGGA GCGCCTGAGG 
AGGACCTTTG TCAACGGAAC TCGGACACAG CCACCCAGCT GGCTGGAGCT GCAGGCCACC 
AAGTCCAAGA AGCCCATCAT GTTGCCCGTG ACCTTCATGG ATGGGACCAC CAAGACCCTG 
CTAACAGATT CAGCAACTAC AGCCAGGGAG CTGTGCAATG CTCTGGCTGA CAAGATCTCA 
CTCAAGGACC GCTTTGGCTT CTCCCTCTAC ATCGCTCTGT TCGATAAGGT GTCCTCCCTG 
GGCAGCGGCA GTGACCATGT CATGGATGCC ATCTCTCAGT GTGAGCAGTA CGCCAAGGAG 
CAGGGTGCTC AGGAGCGCAA CGCCCCATGG AGGCTCTTCT TTAGAAAGGA GGTCTTCACA 
CCCTGGCACA ACCCCTCGGA GGACAACGTG GCCACGAACC TCATCTACCA GCAGGTGGTG 
CGAGGAGTCA AGTTTGGGGA GTACAGGTGT GAGAAGGAGG ACGACCTGGC TGAGCTGGCT 
TCTCAGCAGT ACTTTGTGGA CTATGGTTCT GAGATGATTC TGGAGCGCCT GCTGAGCCTC 
GTGCCCACTT ACATCCCTGA CCGTGAGATC ACACCGCTGA AGAATCTTGA GAAGTGGGCA 
CAGCTGGCCA TTGCTGCCCA CAAGAAGGGA ATTTATGCCC AGAGGAGAAC TGACTCCCAG 
AAGGTCAAAG AGGATGTGGT CAATTATGCC CGTTTCAAGT GGCCCTTGCT CTTCTCCAGG 
TTTTACGAAG CTTACAAATT CTCAGGCCCT CCCCTCCCCA AGAGCGACGT CATCGTGGCT 
GTCAACTGGA CGGGTGTGTA CTTCGTGGAC GAGCAGGAGC AGGTGCTTCT GGAGCTGTCC 
TTCCCGGAGA TCATGGCTGT GTCCAGCAGT AGGGAGTGCC GCGTCTTGCT CTCACTGGGC 
TGCTCTGACT TGGGCTGTGC TACTTGTCAA TCGGGCCGGG CAGGGCTGAC CCCGGCTGGA 
CCCTGTTCTC CGTGTTGGTC CTGTAGGGGA ACAAAGATGA TGGCCCCCAG CTTTACCCTG 
GCCACCATCA AAGGAGATGA GTACACCTTC ACATCCAGCA ATGCTGAGGA CATCCGTGAC 
CTGGTGGTCA CCTTTCTGGA GGGGCTACGG AAGAGGTCTA AGTATGTGGT GGCACTGCAG 
GACAATCCTA ACCCTGCTGG TGAGGAGTCA GGCTTCCTCA GCTTCGCCAA GGGAGACCTC 
ATCATCCTTG ACCATGATAC TGGTGAGCAG GTCATGAACT CAGGCTGGGC CAACGGCATC 
AACGAGAGGA CCAAGCAGCG CGGCGACTTC CCCACTGACT GTGTATACGT CATGCCCACT 
GTCACCTTGC CACCAAGGGA GATTGTGGCC CTGGTCACTA TGACCCCAGA CCAGAGGCAG 
GATGTCGTCC GGCTCCTGCA GCTTCGCACA GCAGAGCCAG AGGTGCGCGC CAAGCCCTAC 
ACGCTAGAGG AGTTCTCCTA CGACTACTTC AGGCCCCCAC CCAAGCACAC GCTGAGCCGT 
GTCATGGTGT CCAAGGCCCG CGGTAAGGAC AGGCTGTGGA GCCACACACG AGAGCCCCTC 
AAGCAGGCCC TGCTCAAGAA GATCCTGGGC AGTGAAGAAC TCTCCCAGGA AGCCTGCATG 
GCCTTTGTAG CTGTGCTCAA GTACATGGGC GACTACCCAT CCAAGAGGAT GCGATCCGTC 
AATGAGCTCA CTGACCAGAT CTTTGAGTGG GCACTCAAGG CTGAGCCCCT CAAGGATGAG 
GCCTACGTGC AGATCCTGAA GCAGCTGACT GACAATCACA TCAGGTACAG CGAAGAGAGG 
GGCTGGGAAC TGCTGTGGCT GTGCACGGGC CTCTTCCCGC CCAGCAACAT CCTCCTGCCT 
CATGTTCAGC GGTTTCTGCA GTCCCGCAAG CACTGTCCTC TTGCCATTGA CTGCCTGCAG 
AGGCTCCAGA AAGCCCTGAG AAATGGCTCC CGGAAGTACC CTCCGCACCT GGTGGAGGTG 
GAGGCCATCC AACATAAGAC TACCCAGATC TTCCACAAGG TCTACTTCCC CGATGACACG 
GACGAGGCTT TTGAGGTGGA GTCCAGCACC AAGGCCAAGG ACTTCTGCCA GAACATCGCC 
AGCCGGCTGC TGCTCAAGTC TTCCGAGGGA TTCAGCCTTT TTGTCAAAAT CGCAGATAAG 
GTCATCAGCG TCCCAGAGAA TGATTTCTTC TTTGACTTTG TCCGACACCT GACAGACTGG 
ATAAAGAAAG CACGGCCCAT CAAGGACGGA ATCGTGCCCT CACTAACCTA CCAGGTGTTC 
TTCATGAAGA AGCTGTGGAC CACCACAGTG CCGGGCAAGG ACCCCATGGC TGACTCCATC 
TTCCACTATT ACCAGGAACT GCCCAAATAT CTCCGAGGCT ACCACAAGTG CACCCGGGAG 
GAGGTGCTGC AGCTGGGCGC ACTCATCTAC AGGGTCAAGT TTGAGGAGGA CAAATCCTAC 
TTCCCTAGCA TCCCCAAGTT GCTGAGGGAG CTGGTACCCC AGGACCTAAT CCGGCAGGTC 
TCACCTGATG ACTGGAAACG GTCTATTGTC GCCTACTTCA ACAAACATGC GGGGAAGTCC 
AAGGAGGAAG CCAAGCTGGC CTTCCTCAAA CTCATCTTCA AGTGGCCCAC CTTTGGCTCA 
GCCTTCTTTG AGGTGAAGCA AACTACAGAA CCAAACTTCC CAGAGATTCT CTTAATTGCC 
ATCAACAAGT ACGGGGTCAG CCTCATCGAT CCCAGAACCA AGGACATCCT GACTACTCAC 
CCCTTCACCA AGATCTCCAA CTGGAGTAGT GGCAACACCT ACTTCCACAT CACCATTGGG 
AACTTGGTCC GTGGGAGCAA ACTGCTCTGT GAGACATCGC TGGGATACAA AATGGATGAT 
CTTCTGACTT CCTACATCAG CCAGATGCTC ACAGCCATGA GCAAGCAGAG GAACTCCAGG 
AGTGGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507491.1
CDS	221..6712
Translation	MHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_006507428.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507428.3

ORF Insert Sequence:

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACCAGCTGT 
GGAGGACGGA CGTACACAGG TTCCATCCTG GTGGCCGTGA ACCCCTACCA GCTGCTCTCC 
ATCTACTCGC CAGAGCACAT CCGCCAGTAC ACCAACAAGA AGATAGGGGA GATGCCCCCC 
CACATCTTCG CCATTGCTGA CAACTGCTAC TTCAACATGA AACGCAACAA CCGGGACCAG 
TGCTGTATTA TCAGCGGGGA GTCGGGAGCT GGCAAGACAG AGAGCACAAA GTTGATCCTG 
CAGTTCCTGG CAGCCATCAG TGGACAGCAC TCATGGATCG AGCAGCAGGT GCTGGAGGCC 
ACCCCGATCC TGGAAGCATT TGGGAACGCC AAGACCATCC GCAACGACAA CTCTAGCCGC 
TTTGGCAAGT ACATTGACAT CCACTTTAAC AAGCGTGGTG CCATCGAGGG CGCCAAAATA 
GAGCAATACC TGCTGGAGAA GTCACGTGTC TGCCGCCAGG CCCCTGACGA GAGGAACTAT 
CACGTGTTCT ACTGTATGCT GGAGGGCATG AATGAGGAGG AGAAGAAGAA ACTGGGCCTA 
GGCCAGGCCG CTGACTACAA CTACTTGGCC ATGGGTAACT GCATCACCTG TGAGGGCCGC 
GTGGACAGTC AGGAGTATGC CAACATCCGC TCTGCCATGA AGGTTCTCAT GTTCACAGAC 
ACGGAGAACT GGGAGATCTC GAAGCTTCTG GCTGCCATCC TACACATGGG CAATCTGCAG 
TATGAGGCCC GGACATTTGA GAACTTGGAT GCGTGTGAAG TCCTCTTCTC CCCATCGCTG 
GCCACGGCAG CTTCTCTGCT CGAGGTGAAC CCCCCAGACC TGATGAGCTG CCTCACCAGC 
CGCACCCTCA TCACCCGTGG GGAGACGGTG TCCACCCCTC TCAGCAGGGA ACAGGCGCTG 
GATGTGCGAG ATGCCTTTGT CAAGGGCATC TATGGGCGGC TCTTTGTGTG GATTGTGGAG 
AAGATCAACG CAGCAATCTA CAAGCCACCC CCCCTGGAAG TGAAGAACTC TCGCCGGTCC 
ATCGGTCTCC TGGACATCTT TGGATTTGAG AACTTCACTG TGAACAGCTT CGAGCAGCTC 
TGCATTAACT TTGCCAATGA GCACCTGCAG CAATTCTTCG TGCGGCACGT GTTCAAGCTG 
GAGCAGGAGG AGTACGACCT GGAGAGCATC GACTGGTTGC ACATTGAGTT CACTGACAAC 
CAGGAAGCAC TGGACATGAT TGCCAACCGG CCTATGAACG TCATCTCCCT CATCGATGAG 
GAGAGCAAGT TCCCCAAGGG CACGGATGCC ACCATGCTGC ATAAGCTGAA CTCACAGCAC 
AAGCTCAATG CCAACTACGT GCCACCCAAG AACAGCCACG AGACCCAGTT TGGAATCAAC 
CACTTTGCGG GTGTTGTCTA TTATGAGAGT CAAGGCTTCC TGGAGAAGAA CCGAGACACC 
CTGCATGGGG ACATCATCCA GCTGGTCCAC TCTTCCCGGA ACAAGTTCAT AAAGCAGATT 
TTCCAAGCTG ACGTTGCCAT GGGTGCCGAG ACCAGGAAGC GCTCGCCTAC ACTCAGCAGC 
CAGTTCAAGC GGTCTCTGGA GCTGCTGATG CGCACACTGG GCGCCTGCCA GCCCTTCTTT 
GTGCGTTGTA TCAAACCCAA TGAGTTCAAG AAGCCCATGC TCTTCGACCG GCACTTGTGT 
GTACGCCAGC TGCGATATTC GGGCATGATG GAGACAATCC GCATCCGCCA CGCAGGCTAC 
CCCATTCGCT ACAGCTTTGT GGAGTTTGTG GAGCGCTACC GGGTACTGCT GCCTGGTGTG 
AAGCCAGCAT ACAAGCAGGG TGACCTCCGA GGGACATGCC AGCGCATGGC TGAGGCTGTG 
CTGGGCACGC ACGATGACTG GCAGATTGGC AAAACCAAGA TCTTTCTGAA GGACCACCAT 
GACATGTTGC TGGAGGTGGA GCGGGACAAG GCCATCACAG ACAGAGTCAT TCTCCTCCAG 
AAGGTTATCC GGGGCTTCAA AGACAGGTCC AACTTCCTGA GACTGAAGAG TGCTGCCACA 
CTGATCCAGA GGCACTGGCG GGGCCACCAC TGTAGGAAAA ACTATGAGCT GATTCGTCTT 
GGCTTCCTGC GGCTGCAGGC CCTGCACCGC TCCCGGAAGC TGCACAAGCA GTACCGCCTG 
GCCAGACAGC GCATAATAGA GTTCCAGGCC CGCTGCCGGG CCTATCTGGT GCGCAAGGCC 
TTCCGCCACC GCCTCTGGGC CGTGATCACC GTGCAGGCCT ATGCCCGAGG CATGATTGCC 
CGCCGGCTAC ACCGCCGCCT CCGGGTTGAG TACCAGCGGC GCCTCGAGGC AGAGAGGATG 
CGTCTGGCAG AGGAGGAGAA ACTCCGAAAG GAGATGAGTG CCAAGAAGGC CAAAGAGGAG 
GCTGAGCGCA AGCATCAGGA GCGCCTGGCT CAGCTAGCCC GCGAGGATGC GGAGCGGGAA 
CTGAAGGAGA AGGAGGAGGC TCGGAGGAAG AAGGAACTGC TGGAGCAGAT GGAGAAGGCC 
CGCCACGAAC CCATCAACCA CTCAGATATG GTGGACAAGA TGTTTGGCTT CCTGGGGACT 
TCAGGCAGCC TGCCAGGCCA GGAAGGCCAG GCGCCTAGTG GCTTTGAGGA CCTAGAGCGC 
GGACGGAGGG AGATGGTGGA AGAGGATGTT GACGCTGCCC TGCCCCTGCC TGATGAAGAC 
GAGGAGGACC TTTCTGAGTA CAAATTCGCC AAGTTTGCTG CCACCTACTT CCAGGGCACA 
ACCACACACT CCTACACCCG GAGGCCTCTC AAGCAGCCGC TGCTCTACCA CGACGATGAG 
GGTGACCAGC TGGCGGCGCT GGCTGTCTGG ATCACCATCC TCCGGTTCAT GGGGGACCTC 
CCAGAGCCCA AGTACCACAC AGCCATGAGC GACGGCAGTG AGAAGATCCC AGTGATGACT 
AAGATCTACG AGACCCTAGG CAAGAAGACA TATAAGAGGG AGCTGCAGGC CTTGCAGGGC 
GAGGGCGAGA CCCAGCTCCC TGAGGGGCAG AAGAAGACCA GTGTGAGACA CAAGTTGGTA 
CACTTGACAC TGAAGAAAAA GTCCAAACTC ACAGAAGAGG TGACCAAGAG GCTGAACGAT 
GGGGAATCCA CGGTACAGGG CAACAGCATG CTGGAGGATC GGCCCACCTC AAATCTAGAG 
AAGCTGCACT TCATCATCGG CAACGGCATC CTGCGGCCTG CGCTGCGGGA CGAGATTTAC 
TGCCAGATCA GTAAGCAGCT CACACACAAC CCATCCAAGA GCAGCTATGC CAGGGGCTGG 
ATCCTCGTGT CGCTCTGTGT GGGCTGCTTC GCCCCCTCTG AGAAGTTCGT TAAGTACCTG 
CGGAACTTCA TCCACGGAGG CCCACCTGGC TATGCTCCTT ACTGTGAGGA GCGCCTGAGG 
AGGACCTTTG TCAACGGAAC TCGGACACAG CCACCCAGCT GGCTGGAGCT GCAGGCCACC 
AAGTCCAAGA AGCCCATCAT GTTGCCCGTG ACCTTCATGG ATGGGACCAC CAAGACCCTG 
CTAACAGATT CAGCAACTAC AGCCAGGGAG CTGTGCAATG CTCTGGCTGA CAAGATCTCA 
CTCAAGGACC GCTTTGGCTT CTCCCTCTAC ATCGCTCTGT TCGATAAGGT GTCCTCCCTG 
GGCAGCGGCA GTGACCATGT CATGGATGCC ATCTCTCAGT GTGAGCAGTA CGCCAAGGAG 
CAGGGTGCTC AGGAGCGCAA CGCCCCATGG AGGCTCTTCT TTAGAAAGGA GGTCTTCACA 
CCCTGGCACA ACCCCTCGGA GGACAACGTG GCCACGAACC TCATCTACCA GCAGGTGGTG 
CGAGGAGTCA AGTTTGGGGA GTACAGGTGT GAGAAGGAGG ACGACCTGGC TGAGCTGGCT 
TCTCAGCAGT ACTTTGTGGA CTATGGTTCT GAGATGATTC TGGAGCGCCT GCTGAGCCTC 
GTGCCCACTT ACATCCCTGA CCGTGAGATC ACACCGCTGA AGAATCTTGA GAAGTGGGCA 
CAGCTGGCCA TTGCTGCCCA CAAGAAGGGA ATTTATGCCC AGAGGAGAAC TGACTCCCAG 
AAGGTCAAAG AGGATGTGGT CAATTATGCC CGTTTCAAGT GGCCCTTGCT CTTCTCCAGG 
TTTTACGAAG CTTACAAATT CTCAGGCCCT CCCCTCCCCA AGAGCGACGT CATCGTGGCT 
GTCAACTGGA CGGGTGTGTA CTTCGTGGAC GAGCAGGAGC AGGTGCTTCT GGAGCTGTCC 
TTCCCGGAGA TCATGGCTGT GTCCAGCAGT AGGGAGTGCC GCGTCTTGCT CTCACTGGGC 
TGCTCTGACT TGGGCTGTGC TACTTGTCAA TCGGGCCGGG CAGGGCTGAC CCCGGCTGGA 
CCCTGTTCTC CGTGTTGGTC CTGTAGGGGA ACAAAGATGA TGGCCCCCAG CTTTACCCTG 
GCCACCATCA AAGGAGATGA GTACACCTTC ACATCCAGCA ATGCTGAGGA CATCCGTGAC 
CTGGTGGTCA CCTTTCTGGA GGGGCTACGG AAGAGGTCTA AGTATGTGGT GGCACTGCAG 
GACAATCCTA ACCCTGCTGG TGAGGAGTCA GGCTTCCTCA GCTTCGCCAA GGGAGACCTC 
ATCATCCTTG ACCATGATAC TGGTGAGCAG GTCATGAACT CAGGCTGGGC CAACGGCATC 
AACGAGAGGA CCAAGCAGCG CGGCGACTTC CCCACTGACT GTGTATACGT CATGCCCACT 
GTCACCTTGC CACCAAGGGA GATTGTGGCC CTGGTCACTA TGACCCCAGA CCAGAGGCAG 
GATGTCGTCC GGCTCCTGCA GCTTCGCACA GCAGAGCCAG AGGTGCGCGC CAAGCCCTAC 
ACGCTAGAGG AGTTCTCCTA CGACTACTTC AGGCCCCCAC CCAAGCACAC GCTGAGCCGT 
GTCATGGTGT CCAAGGCCCG CGGTAAGGAC AGGCTGTGGA GCCACACACG AGAGCCCCTC 
AAGCAGGCCC TGCTCAAGAA GATCCTGGGC AGTGAAGAAC TCTCCCAGGA AGCCTGCATG 
GCCTTTGTAG CTGTGCTCAA GTACATGGGC GACTACCCAT CCAAGAGGAT GCGATCCGTC 
AATGAGCTCA CTGACCAGAT CTTTGAGTGG GCACTCAAGG CTGAGCCCCT CAAGGATGAG 
GCCTACGTGC AGATCCTGAA GCAGCTGACT GACAATCACA TCAGGTACAG CGAAGAGAGG 
GGCTGGGAAC TGCTGTGGCT GTGCACGGGC CTCTTCCCGC CCAGCAACAT CCTCCTGCCT 
CATGTTCAGC GGTTTCTGCA GTCCCGCAAG CACTGTCCTC TTGCCATTGA CTGCCTGCAG 
AGGCTCCAGA AAGCCCTGAG AAATGGCTCC CGGAAGTACC CTCCGCACCT GGTGGAGGTG 
GAGGCCATCC AACATAAGAC TACCCAGATC TTCCACAAGG TCTACTTCCC CGATGACACG 
GACGAGGCTT TTGAGGTGGA GTCCAGCACC AAGGCCAAGG ACTTCTGCCA GAACATCGCC 
AGCCGGCTGC TGCTCAAGTC TTCCGAGGGA TTCAGCCTTT TTGTCAAAAT CGCAGATAAG 
GTCATCAGCG TCCCAGAGAA TGATTTCTTC TTTGACTTTG TCCGACACCT GACAGACTGG 
ATAAAGAAAG CACGGCCCAT CAAGGACGGA ATCGTGCCCT CACTAACCTA CCAGGTGTTC 
TTCATGAAGA AGCTGTGGAC CACCACAGTG CCGGGCAAGG ACCCCATGGC TGACTCCATC 
TTCCACTATT ACCAGGAACT GCCCAAATAT CTCCGAGGCT ACCACAAGTG CACCCGGGAG 
GAGGTGCTGC AGCTGGGCGC ACTCATCTAC AGGGTCAAGT TTGAGGAGGA CAAATCCTAC 
TTCCCTAGCA TCCCCAAGTT GCTGAGGGAG CTGGTACCCC AGGACCTAAT CCGGCAGGTC 
TCACCTGATG ACTGGAAACG GTCTATTGTC GCCTACTTCA ACAAACATGC GGGGAAGTCC 
AAGGAGGAAG CCAAGCTGGC CTTCCTCAAA CTCATCTTCA AGTGGCCCAC CTTTGGCTCA 
GCCTTCTTTG AGGTGAAGCA AACTACAGAA CCAAACTTCC CAGAGATTCT CTTAATTGCC 
ATCAACAAGT ACGGGGTCAG CCTCATCGAT CCCAGAACCA AGGACATCCT GACTACTCAC 
CCCTTCACCA AGATCTCCAA CTGGAGTAGT GGCAACACCT ACTTCCACAT CACCATTGGG 
AACTTGGTCC GTGGGAGCAA ACTGCTCTGT GAGACATCGC TGGGATACAA AATGGATGAT 
CTTCTGACTT CCTACATCAG CCAGATGCTC ACAGCCATGA GCAAGCAGAG GAACTCCAGG 
AGTGGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_006507429.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507492.1 Mus musculus myosin VIIA (Myo7a), transcript variant X7, mRNA.

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CloneID	OMu39359
Clone ID Related Accession (Same CDS sequence)	XM_006507429.2 , XM_006507429.3
Accession Version	XM_006507429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACGTACACA 
GGTTCCATCC TGGTGGCCGT GAACCCCTAC CAGCTGCTCT CCATCTACTC GCCAGAGCAC 
ATCCGCCAGT ACACCAACAA GAAGATAGGG GAGATGCCCC CCCACATCTT CGCCATTGCT 
GACAACTGCT ACTTCAACAT GAAACGCAAC AACCGGGACC AGTGCTGTAT TATCAGCGGG 
GAGTCGGGAG CTGGCAAGAC AGAGAGCACA AAGTTGATCC TGCAGTTCCT GGCAGCCATC 
AGTGGACAGC ACTCATGGAT CGAGCAGCAG GTGCTGGAGG CCACCCCGAT CCTGGAAGCA 
TTTGGGAACG CCAAGACCAT CCGCAACGAC AACTCTAGCC GCTTTGGCAA GTACATTGAC 
ATCCACTTTA ACAAGCGTGG TGCCATCGAG GGCGCCAAAA TAGAGCAATA CCTGCTGGAG 
AAGTCACGTG TCTGCCGCCA GGCCCCTGAC GAGAGGAACT ATCACGTGTT CTACTGTATG 
CTGGAGGGCA TGAATGAGGA GGAGAAGAAG AAACTGGGCC TAGGCCAGGC CGCTGACTAC 
AACTACTTGG CCATGGGTAA CTGCATCACC TGTGAGGGCC GCGTGGACAG TCAGGAGTAT 
GCCAACATCC GCTCTGCCAT GAAGGTTCTC ATGTTCACAG ACACGGAGAA CTGGGAGATC 
TCGAAGCTTC TGGCTGCCAT CCTACACATG GGCAATCTGC AGTATGAGGC CCGGACATTT 
GAGAACTTGG ATGCGTGTGA AGTCCTCTTC TCCCCATCGC TGGCCACGGC AGCTTCTCTG 
CTCGAGGTGA ACCCCCCAGA CCTGATGAGC TGCCTCACCA GCCGCACCCT CATCACCCGT 
GGGGAGACGG TGTCCACCCC TCTCAGCAGG GAACAGGCGC TGGATGTGCG AGATGCCTTT 
GTCAAGGGCA TCTATGGGCG GCTCTTTGTG TGGATTGTGG AGAAGATCAA CGCAGCAATC 
TACAAGCCAC CCCCCCTGGA AGTGAAGAAC TCTCGCCGGT CCATCGGTCT CCTGGACATC 
TTTGGATTTG AGAACTTCAC TGTGAACAGC TTCGAGCAGC TCTGCATTAA CTTTGCCAAT 
GAGCACCTGC AGCAATTCTT CGTGCGGCAC GTGTTCAAGC TGGAGCAGGA GGAGTACGAC 
CTGGAGAGCA TCGACTGGTT GCACATTGAG TTCACTGACA ACCAGGAAGC ACTGGACATG 
ATTGCCAACC GGCCTATGAA CGTCATCTCC CTCATCGATG AGGAGAGCAA GTTCCCCAAG 
GGCACGGATG CCACCATGCT GCATAAGCTG AACTCACAGC ACAAGCTCAA TGCCAACTAC 
GTGCCACCCA AGAACAGCCA CGAGACCCAG TTTGGAATCA ACCACTTTGC GGGTGTTGTC 
TATTATGAGA GTCAAGGCTT CCTGGAGAAG AACCGAGACA CCCTGCATGG GGACATCATC 
CAGCTGGTCC ACTCTTCCCG GAACAAGTTC ATAAAGCAGA TTTTCCAAGC TGACGTTGCC 
ATGGGTGCCG AGACCAGGAA GCGCTCGCCT ACACTCAGCA GCCAGTTCAA GCGGTCTCTG 
GAGCTGCTGA TGCGCACACT GGGCGCCTGC CAGCCCTTCT TTGTGCGTTG TATCAAACCC 
AATGAGTTCA AGAAGCCCAT GCTCTTCGAC CGGCACTTGT GTGTACGCCA GCTGCGATAT 
TCGGGCATGA TGGAGACAAT CCGCATCCGC CACGCAGGCT ACCCCATTCG CTACAGCTTT 
GTGGAGTTTG TGGAGCGCTA CCGGGTACTG CTGCCTGGTG TGAAGCCAGC ATACAAGCAG 
GGTGACCTCC GAGGGACATG CCAGCGCATG GCTGAGGCTG TGCTGGGCAC GCACGATGAC 
TGGCAGATTG GCAAAACCAA GATCTTTCTG AAGGACCACC ATGACATGTT GCTGGAGGTG 
GAGCGGGACA AGGCCATCAC AGACAGAGTC ATTCTCCTCC AGAAGGTTAT CCGGGGCTTC 
AAAGACAGGT CCAACTTCCT GAGACTGAAG AGTGCTGCCA CACTGATCCA GAGGCACTGG 
CGGGGCCACC ACTGTAGGAA AAACTATGAG CTGATTCGTC TTGGCTTCCT GCGGCTGCAG 
GCCCTGCACC GCTCCCGGAA GCTGCACAAG CAGTACCGCC TGGCCAGACA GCGCATAATA 
GAGTTCCAGG CCCGCTGCCG GGCCTATCTG GTGCGCAAGG CCTTCCGCCA CCGCCTCTGG 
GCCGTGATCA CCGTGCAGGC CTATGCCCGA GGCATGATTG CCCGCCGGCT ACACCGCCGC 
CTCCGGGTTG AGTACCAGCG GCGCCTCGAG GCAGAGAGGA TGCGTCTGGC AGAGGAGGAG 
AAACTCCGAA AGGAGATGAG TGCCAAGAAG GCCAAAGAGG AGGCTGAGCG CAAGCATCAG 
GAGCGCCTGG CTCAGCTAGC CCGCGAGGAT GCGGAGCGGG AACTGAAGGA GAAGGAGGAG 
GCTCGGAGGA AGAAGGAACT GCTGGAGCAG ATGGAGAAGG CCCGCCACGA ACCCATCAAC 
CACTCAGATA TGGTGGACAA GATGTTTGGC TTCCTGGGGA CTTCAGGCAG CCTGCCAGGC 
CAGGAAGGCC AGGCGCCTAG TGGCTTTGAG GACCTAGAGC GCGGACGGAG GGAGATGGTG 
GAAGAGGATG TTGACGCTGC CCTGCCCCTG CCTGATGAAG ACGAGGAGGA CCTTTCTGAG 
TACAAATTCG CCAAGTTTGC TGCCACCTAC TTCCAGGGCA CAACCACACA CTCCTACACC 
CGGAGGCCTC TCAAGCAGCC GCTGCTCTAC CACGACGATG AGGGTGACCA GCTGGCGGCG 
CTGGCTGTCT GGATCACCAT CCTCCGGTTC ATGGGGGACC TCCCAGAGCC CAAGTACCAC 
ACAGCCATGA GCGACGGCAG TGAGAAGATC CCAGTGATGA CTAAGATCTA CGAGACCCTA 
GGCAAGAAGA CATATAAGAG GGAGCTGCAG GCCTTGCAGG GCGAGGGCGA GACCCAGCTC 
CCTGAGGGGC AGAAGAAGAC CAGTGTGAGA CACAAGTTGG TACACTTGAC ACTGAAGAAA 
AAGTCCAAAC TCACAGAAGA GGTGACCAAG AGGCTGAACG ATGGGGAATC CACGGTACAG 
GGCAACAGCA TGCTGGAGGA TCGGCCCACC TCAAATCTAG AGAAGCTGCA CTTCATCATC 
GGCAACGGCA TCCTGCGGCC TGCGCTGCGG GACGAGATTT ACTGCCAGAT CAGTAAGCAG 
CTCACACACA ACCCATCCAA GAGCAGCTAT GCCAGGGGCT GGATCCTCGT GTCGCTCTGT 
GTGGGCTGCT TCGCCCCCTC TGAGAAGTTC GTTAAGTACC TGCGGAACTT CATCCACGGA 
GGCCCACCTG GCTATGCTCC TTACTGTGAG GAGCGCCTGA GGAGGACCTT TGTCAACGGA 
ACTCGGACAC AGCCACCCAG CTGGCTGGAG CTGCAGGCCA CCAAGTCCAA GAAGCCCATC 
ATGTTGCCCG TGACCTTCAT GGATGGGACC ACCAAGACCC TGCTAACAGA TTCAGCAACT 
ACAGCCAGGG AGCTGTGCAA TGCTCTGGCT GACAAGATCT CACTCAAGGA CCGCTTTGGC 
TTCTCCCTCT ACATCGCTCT GTTCGATAAG GTGTCCTCCC TGGGCAGCGG CAGTGACCAT 
GTCATGGATG CCATCTCTCA GTGTGAGCAG TACGCCAAGG AGCAGGGTGC TCAGGAGCGC 
AACGCCCCAT GGAGGCTCTT CTTTAGAAAG GAGGTCTTCA CACCCTGGCA CAACCCCTCG 
GAGGACAACG TGGCCACGAA CCTCATCTAC CAGCAGGTGG TGCGAGGAGT CAAGTTTGGG 
GAGTACAGGT GTGAGAAGGA GGACGACCTG GCTGAGCTGG CTTCTCAGCA GTACTTTGTG 
GACTATGGTT CTGAGATGAT TCTGGAGCGC CTGCTGAGCC TCGTGCCCAC TTACATCCCT 
GACCGTGAGA TCACACCGCT GAAGAATCTT GAGAAGTGGG CACAGCTGGC CATTGCTGCC 
CACAAGAAGG GAATTTATGC CCAGAGGAGA ACTGACTCCC AGAAGGTCAA AGAGGATGTG 
GTCAATTATG CCCGTTTCAA GTGGCCCTTG CTCTTCTCCA GGTTTTACGA AGCTTACAAA 
TTCTCAGGCC CTCCCCTCCC CAAGAGCGAC GTCATCGTGG CTGTCAACTG GACGGGTGTG 
TACTTCGTGG ACGAGCAGGA GCAGGTGCTT CTGGAGCTGT CCTTCCCGGA GATCATGGCT 
GTGTCCAGCA GTAGGGAGTG CCGCGTCTTG CTCTCACTGG GCTGCTCTGA CTTGGGCTGT 
GCTACTTGTC AATCGGGCCG GGCAGGGCTG ACCCCGGCTG GACCCTGTTC TCCGTGTTGG 
TCCTGTAGGG GAACAAAGAT GATGGCCCCC AGCTTTACCC TGGCCACCAT CAAAGGAGAT 
GAGTACACCT TCACATCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTTCTG 
GAGGGGCTAC GGAAGAGGTC TAAGTATGTG GTGGCACTGC AGGACAATCC TAACCCTGCT 
GGTGAGGAGT CAGGCTTCCT CAGCTTCGCC AAGGGAGACC TCATCATCCT TGACCATGAT 
ACTGGTGAGC AGGTCATGAA CTCAGGCTGG GCCAACGGCA TCAACGAGAG GACCAAGCAG 
CGCGGCGACT TCCCCACTGA CTGTGTATAC GTCATGCCCA CTGTCACCTT GCCACCAAGG 
GAGATTGTGG CCCTGGTCAC TATGACCCCA GACCAGAGGC AGGATGTCGT CCGGCTCCTG 
CAGCTTCGCA CAGCAGAGCC AGAGGTGCGC GCCAAGCCCT ACACGCTAGA GGAGTTCTCC 
TACGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGCGGTAAGG ACAGGCTGTG GAGCCACACA CGAGAGCCCC TCAAGCAGGC CCTGCTCAAG 
AAGATCCTGG GCAGTGAAGA ACTCTCCCAG GAAGCCTGCA TGGCCTTTGT AGCTGTGCTC 
AAGTACATGG GCGACTACCC ATCCAAGAGG ATGCGATCCG TCAATGAGCT CACTGACCAG 
ATCTTTGAGT GGGCACTCAA GGCTGAGCCC CTCAAGGATG AGGCCTACGT GCAGATCCTG 
AAGCAGCTGA CTGACAATCA CATCAGGTAC AGCGAAGAGA GGGGCTGGGA ACTGCTGTGG 
CTGTGCACGG GCCTCTTCCC GCCCAGCAAC ATCCTCCTGC CTCATGTTCA GCGGTTTCTG 
CAGTCCCGCA AGCACTGTCC TCTTGCCATT GACTGCCTGC AGAGGCTCCA GAAAGCCCTG 
AGAAATGGCT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAACATAAG 
ACTACCCAGA TCTTCCACAA GGTCTACTTC CCCGATGACA CGGACGAGGC TTTTGAGGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCAGCCGGCT GCTGCTCAAG 
TCTTCCGAGG GATTCAGCCT TTTTGTCAAA ATCGCAGATA AGGTCATCAG CGTCCCAGAG 
AATGATTTCT TCTTTGACTT TGTCCGACAC CTGACAGACT GGATAAAGAA AGCACGGCCC 
ATCAAGGACG GAATCGTGCC CTCACTAACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACAG TGCCGGGCAA GGACCCCATG GCTGACTCCA TCTTCCACTA TTACCAGGAA 
CTGCCCAAAT ATCTCCGAGG CTACCACAAG TGCACCCGGG AGGAGGTGCT GCAGCTGGGC 
GCACTCATCT ACAGGGTCAA GTTTGAGGAG GACAAATCCT ACTTCCCTAG CATCCCCAAG 
TTGCTGAGGG AGCTGGTACC CCAGGACCTA ATCCGGCAGG TCTCACCTGA TGACTGGAAA 
CGGTCTATTG TCGCCTACTT CAACAAACAT GCGGGGAAGT CCAAGGAGGA AGCCAAGCTG 
GCCTTCCTCA AACTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT TGAGGTGAAG 
CAAACTACAG AACCAAACTT CCCAGAGATT CTCTTAATTG CCATCAACAA GTACGGGGTC 
AGCCTCATCG ATCCCAGAAC CAAGGACATC CTGACTACTC ACCCCTTCAC CAAGATCTCC 
AACTGGAGTA GTGGCAACAC CTACTTCCAC ATCACCATTG GGAACTTGGT CCGTGGGAGC 
AAACTGCTCT GTGAGACATC GCTGGGATAC AAAATGGATG ATCTTCTGAC TTCCTACATC 
AGCCAGATGC TCACAGCCAT GAGCAAGCAG AGGAACTCCA GGAGTGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507492.1
CDS	231..6704
Translation	MHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_006507429.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507429.3

ORF Insert Sequence:

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACGTACACA 
GGTTCCATCC TGGTGGCCGT GAACCCCTAC CAGCTGCTCT CCATCTACTC GCCAGAGCAC 
ATCCGCCAGT ACACCAACAA GAAGATAGGG GAGATGCCCC CCCACATCTT CGCCATTGCT 
GACAACTGCT ACTTCAACAT GAAACGCAAC AACCGGGACC AGTGCTGTAT TATCAGCGGG 
GAGTCGGGAG CTGGCAAGAC AGAGAGCACA AAGTTGATCC TGCAGTTCCT GGCAGCCATC 
AGTGGACAGC ACTCATGGAT CGAGCAGCAG GTGCTGGAGG CCACCCCGAT CCTGGAAGCA 
TTTGGGAACG CCAAGACCAT CCGCAACGAC AACTCTAGCC GCTTTGGCAA GTACATTGAC 
ATCCACTTTA ACAAGCGTGG TGCCATCGAG GGCGCCAAAA TAGAGCAATA CCTGCTGGAG 
AAGTCACGTG TCTGCCGCCA GGCCCCTGAC GAGAGGAACT ATCACGTGTT CTACTGTATG 
CTGGAGGGCA TGAATGAGGA GGAGAAGAAG AAACTGGGCC TAGGCCAGGC CGCTGACTAC 
AACTACTTGG CCATGGGTAA CTGCATCACC TGTGAGGGCC GCGTGGACAG TCAGGAGTAT 
GCCAACATCC GCTCTGCCAT GAAGGTTCTC ATGTTCACAG ACACGGAGAA CTGGGAGATC 
TCGAAGCTTC TGGCTGCCAT CCTACACATG GGCAATCTGC AGTATGAGGC CCGGACATTT 
GAGAACTTGG ATGCGTGTGA AGTCCTCTTC TCCCCATCGC TGGCCACGGC AGCTTCTCTG 
CTCGAGGTGA ACCCCCCAGA CCTGATGAGC TGCCTCACCA GCCGCACCCT CATCACCCGT 
GGGGAGACGG TGTCCACCCC TCTCAGCAGG GAACAGGCGC TGGATGTGCG AGATGCCTTT 
GTCAAGGGCA TCTATGGGCG GCTCTTTGTG TGGATTGTGG AGAAGATCAA CGCAGCAATC 
TACAAGCCAC CCCCCCTGGA AGTGAAGAAC TCTCGCCGGT CCATCGGTCT CCTGGACATC 
TTTGGATTTG AGAACTTCAC TGTGAACAGC TTCGAGCAGC TCTGCATTAA CTTTGCCAAT 
GAGCACCTGC AGCAATTCTT CGTGCGGCAC GTGTTCAAGC TGGAGCAGGA GGAGTACGAC 
CTGGAGAGCA TCGACTGGTT GCACATTGAG TTCACTGACA ACCAGGAAGC ACTGGACATG 
ATTGCCAACC GGCCTATGAA CGTCATCTCC CTCATCGATG AGGAGAGCAA GTTCCCCAAG 
GGCACGGATG CCACCATGCT GCATAAGCTG AACTCACAGC ACAAGCTCAA TGCCAACTAC 
GTGCCACCCA AGAACAGCCA CGAGACCCAG TTTGGAATCA ACCACTTTGC GGGTGTTGTC 
TATTATGAGA GTCAAGGCTT CCTGGAGAAG AACCGAGACA CCCTGCATGG GGACATCATC 
CAGCTGGTCC ACTCTTCCCG GAACAAGTTC ATAAAGCAGA TTTTCCAAGC TGACGTTGCC 
ATGGGTGCCG AGACCAGGAA GCGCTCGCCT ACACTCAGCA GCCAGTTCAA GCGGTCTCTG 
GAGCTGCTGA TGCGCACACT GGGCGCCTGC CAGCCCTTCT TTGTGCGTTG TATCAAACCC 
AATGAGTTCA AGAAGCCCAT GCTCTTCGAC CGGCACTTGT GTGTACGCCA GCTGCGATAT 
TCGGGCATGA TGGAGACAAT CCGCATCCGC CACGCAGGCT ACCCCATTCG CTACAGCTTT 
GTGGAGTTTG TGGAGCGCTA CCGGGTACTG CTGCCTGGTG TGAAGCCAGC ATACAAGCAG 
GGTGACCTCC GAGGGACATG CCAGCGCATG GCTGAGGCTG TGCTGGGCAC GCACGATGAC 
TGGCAGATTG GCAAAACCAA GATCTTTCTG AAGGACCACC ATGACATGTT GCTGGAGGTG 
GAGCGGGACA AGGCCATCAC AGACAGAGTC ATTCTCCTCC AGAAGGTTAT CCGGGGCTTC 
AAAGACAGGT CCAACTTCCT GAGACTGAAG AGTGCTGCCA CACTGATCCA GAGGCACTGG 
CGGGGCCACC ACTGTAGGAA AAACTATGAG CTGATTCGTC TTGGCTTCCT GCGGCTGCAG 
GCCCTGCACC GCTCCCGGAA GCTGCACAAG CAGTACCGCC TGGCCAGACA GCGCATAATA 
GAGTTCCAGG CCCGCTGCCG GGCCTATCTG GTGCGCAAGG CCTTCCGCCA CCGCCTCTGG 
GCCGTGATCA CCGTGCAGGC CTATGCCCGA GGCATGATTG CCCGCCGGCT ACACCGCCGC 
CTCCGGGTTG AGTACCAGCG GCGCCTCGAG GCAGAGAGGA TGCGTCTGGC AGAGGAGGAG 
AAACTCCGAA AGGAGATGAG TGCCAAGAAG GCCAAAGAGG AGGCTGAGCG CAAGCATCAG 
GAGCGCCTGG CTCAGCTAGC CCGCGAGGAT GCGGAGCGGG AACTGAAGGA GAAGGAGGAG 
GCTCGGAGGA AGAAGGAACT GCTGGAGCAG ATGGAGAAGG CCCGCCACGA ACCCATCAAC 
CACTCAGATA TGGTGGACAA GATGTTTGGC TTCCTGGGGA CTTCAGGCAG CCTGCCAGGC 
CAGGAAGGCC AGGCGCCTAG TGGCTTTGAG GACCTAGAGC GCGGACGGAG GGAGATGGTG 
GAAGAGGATG TTGACGCTGC CCTGCCCCTG CCTGATGAAG ACGAGGAGGA CCTTTCTGAG 
TACAAATTCG CCAAGTTTGC TGCCACCTAC TTCCAGGGCA CAACCACACA CTCCTACACC 
CGGAGGCCTC TCAAGCAGCC GCTGCTCTAC CACGACGATG AGGGTGACCA GCTGGCGGCG 
CTGGCTGTCT GGATCACCAT CCTCCGGTTC ATGGGGGACC TCCCAGAGCC CAAGTACCAC 
ACAGCCATGA GCGACGGCAG TGAGAAGATC CCAGTGATGA CTAAGATCTA CGAGACCCTA 
GGCAAGAAGA CATATAAGAG GGAGCTGCAG GCCTTGCAGG GCGAGGGCGA GACCCAGCTC 
CCTGAGGGGC AGAAGAAGAC CAGTGTGAGA CACAAGTTGG TACACTTGAC ACTGAAGAAA 
AAGTCCAAAC TCACAGAAGA GGTGACCAAG AGGCTGAACG ATGGGGAATC CACGGTACAG 
GGCAACAGCA TGCTGGAGGA TCGGCCCACC TCAAATCTAG AGAAGCTGCA CTTCATCATC 
GGCAACGGCA TCCTGCGGCC TGCGCTGCGG GACGAGATTT ACTGCCAGAT CAGTAAGCAG 
CTCACACACA ACCCATCCAA GAGCAGCTAT GCCAGGGGCT GGATCCTCGT GTCGCTCTGT 
GTGGGCTGCT TCGCCCCCTC TGAGAAGTTC GTTAAGTACC TGCGGAACTT CATCCACGGA 
GGCCCACCTG GCTATGCTCC TTACTGTGAG GAGCGCCTGA GGAGGACCTT TGTCAACGGA 
ACTCGGACAC AGCCACCCAG CTGGCTGGAG CTGCAGGCCA CCAAGTCCAA GAAGCCCATC 
ATGTTGCCCG TGACCTTCAT GGATGGGACC ACCAAGACCC TGCTAACAGA TTCAGCAACT 
ACAGCCAGGG AGCTGTGCAA TGCTCTGGCT GACAAGATCT CACTCAAGGA CCGCTTTGGC 
TTCTCCCTCT ACATCGCTCT GTTCGATAAG GTGTCCTCCC TGGGCAGCGG CAGTGACCAT 
GTCATGGATG CCATCTCTCA GTGTGAGCAG TACGCCAAGG AGCAGGGTGC TCAGGAGCGC 
AACGCCCCAT GGAGGCTCTT CTTTAGAAAG GAGGTCTTCA CACCCTGGCA CAACCCCTCG 
GAGGACAACG TGGCCACGAA CCTCATCTAC CAGCAGGTGG TGCGAGGAGT CAAGTTTGGG 
GAGTACAGGT GTGAGAAGGA GGACGACCTG GCTGAGCTGG CTTCTCAGCA GTACTTTGTG 
GACTATGGTT CTGAGATGAT TCTGGAGCGC CTGCTGAGCC TCGTGCCCAC TTACATCCCT 
GACCGTGAGA TCACACCGCT GAAGAATCTT GAGAAGTGGG CACAGCTGGC CATTGCTGCC 
CACAAGAAGG GAATTTATGC CCAGAGGAGA ACTGACTCCC AGAAGGTCAA AGAGGATGTG 
GTCAATTATG CCCGTTTCAA GTGGCCCTTG CTCTTCTCCA GGTTTTACGA AGCTTACAAA 
TTCTCAGGCC CTCCCCTCCC CAAGAGCGAC GTCATCGTGG CTGTCAACTG GACGGGTGTG 
TACTTCGTGG ACGAGCAGGA GCAGGTGCTT CTGGAGCTGT CCTTCCCGGA GATCATGGCT 
GTGTCCAGCA GTAGGGAGTG CCGCGTCTTG CTCTCACTGG GCTGCTCTGA CTTGGGCTGT 
GCTACTTGTC AATCGGGCCG GGCAGGGCTG ACCCCGGCTG GACCCTGTTC TCCGTGTTGG 
TCCTGTAGGG GAACAAAGAT GATGGCCCCC AGCTTTACCC TGGCCACCAT CAAAGGAGAT 
GAGTACACCT TCACATCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTTCTG 
GAGGGGCTAC GGAAGAGGTC TAAGTATGTG GTGGCACTGC AGGACAATCC TAACCCTGCT 
GGTGAGGAGT CAGGCTTCCT CAGCTTCGCC AAGGGAGACC TCATCATCCT TGACCATGAT 
ACTGGTGAGC AGGTCATGAA CTCAGGCTGG GCCAACGGCA TCAACGAGAG GACCAAGCAG 
CGCGGCGACT TCCCCACTGA CTGTGTATAC GTCATGCCCA CTGTCACCTT GCCACCAAGG 
GAGATTGTGG CCCTGGTCAC TATGACCCCA GACCAGAGGC AGGATGTCGT CCGGCTCCTG 
CAGCTTCGCA CAGCAGAGCC AGAGGTGCGC GCCAAGCCCT ACACGCTAGA GGAGTTCTCC 
TACGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGCGGTAAGG ACAGGCTGTG GAGCCACACA CGAGAGCCCC TCAAGCAGGC CCTGCTCAAG 
AAGATCCTGG GCAGTGAAGA ACTCTCCCAG GAAGCCTGCA TGGCCTTTGT AGCTGTGCTC 
AAGTACATGG GCGACTACCC ATCCAAGAGG ATGCGATCCG TCAATGAGCT CACTGACCAG 
ATCTTTGAGT GGGCACTCAA GGCTGAGCCC CTCAAGGATG AGGCCTACGT GCAGATCCTG 
AAGCAGCTGA CTGACAATCA CATCAGGTAC AGCGAAGAGA GGGGCTGGGA ACTGCTGTGG 
CTGTGCACGG GCCTCTTCCC GCCCAGCAAC ATCCTCCTGC CTCATGTTCA GCGGTTTCTG 
CAGTCCCGCA AGCACTGTCC TCTTGCCATT GACTGCCTGC AGAGGCTCCA GAAAGCCCTG 
AGAAATGGCT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAACATAAG 
ACTACCCAGA TCTTCCACAA GGTCTACTTC CCCGATGACA CGGACGAGGC TTTTGAGGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCAGCCGGCT GCTGCTCAAG 
TCTTCCGAGG GATTCAGCCT TTTTGTCAAA ATCGCAGATA AGGTCATCAG CGTCCCAGAG 
AATGATTTCT TCTTTGACTT TGTCCGACAC CTGACAGACT GGATAAAGAA AGCACGGCCC 
ATCAAGGACG GAATCGTGCC CTCACTAACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACAG TGCCGGGCAA GGACCCCATG GCTGACTCCA TCTTCCACTA TTACCAGGAA 
CTGCCCAAAT ATCTCCGAGG CTACCACAAG TGCACCCGGG AGGAGGTGCT GCAGCTGGGC 
GCACTCATCT ACAGGGTCAA GTTTGAGGAG GACAAATCCT ACTTCCCTAG CATCCCCAAG 
TTGCTGAGGG AGCTGGTACC CCAGGACCTA ATCCGGCAGG TCTCACCTGA TGACTGGAAA 
CGGTCTATTG TCGCCTACTT CAACAAACAT GCGGGGAAGT CCAAGGAGGA AGCCAAGCTG 
GCCTTCCTCA AACTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT TGAGGTGAAG 
CAAACTACAG AACCAAACTT CCCAGAGATT CTCTTAATTG CCATCAACAA GTACGGGGTC 
AGCCTCATCG ATCCCAGAAC CAAGGACATC CTGACTACTC ACCCCTTCAC CAAGATCTCC 
AACTGGAGTA GTGGCAACAC CTACTTCCAC ATCACCATTG GGAACTTGGT CCGTGGGAGC 
AAACTGCTCT GTGAGACATC GCTGGGATAC AAAATGGATG ATCTTCTGAC TTCCTACATC 
AGCCAGATGC TCACAGCCAT GAGCAAGCAG AGGAACTCCA GGAGTGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241688.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239990.1 Mus musculus myosin VIIA (Myo7a), transcript variant X1, mRNA.

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CloneID	OMu68262
Clone ID Related Accession (Same CDS sequence)	XM_011241688.2 , XM_011241688.3
Accession Version	XM_011241688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGAC AGTGGCATCT GGCTCAGGGC TCTGCATCGA CTGGAGCCTG GTACCTCATC 
TTGTTTAGAT ACCGGGACAC AGGAATGCTG CCAAGGTCCT ACAAGGGGGA CTATGTATGG 
ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGAGTGC 
CGCGTCTTGC TCTCACTGGG CTGCTCTGAC TTGGGCTGTG CTACTTGTCA ATCGGGCCGG 
GCAGGGCTGA CCCCGGCTGG ACCCTGTTCT CCGTGTTGGT CCTGTAGGGG AACAAAGATG 
ATGGCCCCCA GCTTTACCCT GGCCACCATC AAAGGAGATG AGTACACCTT CACATCCAGC 
AATGCTGAGG ACATCCGTGA CCTGGTGGTC ACCTTTCTGG AGGGGCTACG GAAGAGGTCT 
AAGTATGTGG TGGCACTGCA GGACAATCCT AACCCTGCTG GTGAGGAGTC AGGCTTCCTC 
AGCTTCGCCA AGGGAGACCT CATCATCCTT GACCATGATA CTGGTGAGCA GGTCATGAAC 
TCAGGCTGGG CCAACGGCAT CAACGAGAGG ACCAAGCAGC GCGGCGACTT CCCCACTGAC 
TGTGTATACG TCATGCCCAC TGTCACCTTG CCACCAAGGG AGATTGTGGC CCTGGTCACT 
ATGACCCCAG ACCAGAGGCA GGATGTCGTC CGGCTCCTGC AGCTTCGCAC AGCAGAGCCA 
GAGGTGCGCG CCAAGCCCTA CACGCTAGAG GAGTTCTCCT ACGACTACTT CAGGCCCCCA 
CCCAAGCACA CGCTGAGCCG TGTCATGGTG TCCAAGGCCC GCGGTAAGGA CAGGCTGTGG 
AGCCACACAC GAGAGCCCCT CAAGCAGGCC CTGCTCAAGA AGATCCTGGG CAGTGAAGAA 
CTCTCCCAGG AAGCCTGCAT GGCCTTTGTA GCTGTGCTCA AGTACATGGG CGACTACCCA 
TCCAAGAGGA TGCGATCCGT CAATGAGCTC ACTGACCAGA TCTTTGAGTG GGCACTCAAG 
GCTGAGCCCC TCAAGGATGA GGCCTACGTG CAGATCCTGA AGCAGCTGAC TGACAATCAC 
ATCAGGTACA GCGAAGAGAG GGGCTGGGAA CTGCTGTGGC TGTGCACGGG CCTCTTCCCG 
CCCAGCAACA TCCTCCTGCC TCATGTTCAG CGGTTTCTGC AGTCCCGCAA GCACTGTCCT 
CTTGCCATTG ACTGCCTGCA GAGGCTCCAG AAAGCCCTGA GAAATGGCTC CCGGAAGTAC 
CCTCCGCACC TGGTGGAGGT GGAGGCCATC CAACATAAGA CTACCCAGAT CTTCCACAAG 
GTCTACTTCC CCGATGACAC GGACGAGGCT TTTGAGGTGG AGTCCAGCAC CAAGGCCAAG 
GACTTCTGCC AGAACATCGC CAGCCGGCTG CTGCTCAAGT CTTCCGAGGG ATTCAGCCTT 
TTTGTCAAAA TCGCAGATAA GGTCATCAGC GTCCCAGAGA ATGATTTCTT CTTTGACTTT 
GTCCGACACC TGACAGACTG GATAAAGAAA GCACGGCCCA TCAAGGACGG AATCGTGCCC 
TCACTAACCT ACCAGGTGTT CTTCATGAAG AAGCTGTGGA CCACCACAGT GCCGGGCAAG 
GACCCCATGG CTGACTCCAT CTTCCACTAT TACCAGGAAC TGCCCAAATA TCTCCGAGGC 
TACCACAAGT GCACCCGGGA GGAGGTGCTG CAGCTGGGCG CACTCATCTA CAGGGTCAAG 
TTTGAGGAGG ACAAATCCTA CTTCCCTAGC ATCCCCAAGT TGCTGAGGGA GCTGGTACCC 
CAGGACCTAA TCCGGCAGGT CTCACCTGAT GACTGGAAAC GGTCTATTGT CGCCTACTTC 
AACAAACATG CGGGGAAGTC CAAGGAGGAA GCCAAGCTGG CCTTCCTCAA ACTCATCTTC 
AAGTGGCCCA CCTTTGGCTC AGCCTTCTTT GAGGTGAAGC AAACTACAGA ACCAAACTTC 
CCAGAGATTC TCTTAATTGC CATCAACAAG TACGGGGTCA GCCTCATCGA TCCCAGAACC 
AAGGACATCC TGACTACTCA CCCCTTCACC AAGATCTCCA ACTGGAGTAG TGGCAACACC 
TACTTCCACA TCACCATTGG GAACTTGGTC CGTGGGAGCA AACTGCTCTG TGAGACATCG 
CTGGGATACA AAATGGATGA TCTTCTGACT TCCTACATCA GCCAGATGCT CACAGCCATG 
AGCAAGCAGA GGAACTCCAG GAGTGGAAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239990.1
CDS	19..6771
Translation	MDRQWHLAQGSASTGAWYLILFRYRDTGMLPRSYKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241688.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241688.3

ORF Insert Sequence:

ATGGATCGAC AGTGGCATCT GGCTCAGGGC TCTGCATCGA CTGGAGCCTG GTACCTCATC 
TTGTTTAGAT ACCGGGACAC AGGAATGCTG CCAAGGTCCT ACAAGGGGGA CTATGTATGG 
ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGAGTGC 
CGCGTCTTGC TCTCACTGGG CTGCTCTGAC TTGGGCTGTG CTACTTGTCA ATCGGGCCGG 
GCAGGGCTGA CCCCGGCTGG ACCCTGTTCT CCGTGTTGGT CCTGTAGGGG AACAAAGATG 
ATGGCCCCCA GCTTTACCCT GGCCACCATC AAAGGAGATG AGTACACCTT CACATCCAGC 
AATGCTGAGG ACATCCGTGA CCTGGTGGTC ACCTTTCTGG AGGGGCTACG GAAGAGGTCT 
AAGTATGTGG TGGCACTGCA GGACAATCCT AACCCTGCTG GTGAGGAGTC AGGCTTCCTC 
AGCTTCGCCA AGGGAGACCT CATCATCCTT GACCATGATA CTGGTGAGCA GGTCATGAAC 
TCAGGCTGGG CCAACGGCAT CAACGAGAGG ACCAAGCAGC GCGGCGACTT CCCCACTGAC 
TGTGTATACG TCATGCCCAC TGTCACCTTG CCACCAAGGG AGATTGTGGC CCTGGTCACT 
ATGACCCCAG ACCAGAGGCA GGATGTCGTC CGGCTCCTGC AGCTTCGCAC AGCAGAGCCA 
GAGGTGCGCG CCAAGCCCTA CACGCTAGAG GAGTTCTCCT ACGACTACTT CAGGCCCCCA 
CCCAAGCACA CGCTGAGCCG TGTCATGGTG TCCAAGGCCC GCGGTAAGGA CAGGCTGTGG 
AGCCACACAC GAGAGCCCCT CAAGCAGGCC CTGCTCAAGA AGATCCTGGG CAGTGAAGAA 
CTCTCCCAGG AAGCCTGCAT GGCCTTTGTA GCTGTGCTCA AGTACATGGG CGACTACCCA 
TCCAAGAGGA TGCGATCCGT CAATGAGCTC ACTGACCAGA TCTTTGAGTG GGCACTCAAG 
GCTGAGCCCC TCAAGGATGA GGCCTACGTG CAGATCCTGA AGCAGCTGAC TGACAATCAC 
ATCAGGTACA GCGAAGAGAG GGGCTGGGAA CTGCTGTGGC TGTGCACGGG CCTCTTCCCG 
CCCAGCAACA TCCTCCTGCC TCATGTTCAG CGGTTTCTGC AGTCCCGCAA GCACTGTCCT 
CTTGCCATTG ACTGCCTGCA GAGGCTCCAG AAAGCCCTGA GAAATGGCTC CCGGAAGTAC 
CCTCCGCACC TGGTGGAGGT GGAGGCCATC CAACATAAGA CTACCCAGAT CTTCCACAAG 
GTCTACTTCC CCGATGACAC GGACGAGGCT TTTGAGGTGG AGTCCAGCAC CAAGGCCAAG 
GACTTCTGCC AGAACATCGC CAGCCGGCTG CTGCTCAAGT CTTCCGAGGG ATTCAGCCTT 
TTTGTCAAAA TCGCAGATAA GGTCATCAGC GTCCCAGAGA ATGATTTCTT CTTTGACTTT 
GTCCGACACC TGACAGACTG GATAAAGAAA GCACGGCCCA TCAAGGACGG AATCGTGCCC 
TCACTAACCT ACCAGGTGTT CTTCATGAAG AAGCTGTGGA CCACCACAGT GCCGGGCAAG 
GACCCCATGG CTGACTCCAT CTTCCACTAT TACCAGGAAC TGCCCAAATA TCTCCGAGGC 
TACCACAAGT GCACCCGGGA GGAGGTGCTG CAGCTGGGCG CACTCATCTA CAGGGTCAAG 
TTTGAGGAGG ACAAATCCTA CTTCCCTAGC ATCCCCAAGT TGCTGAGGGA GCTGGTACCC 
CAGGACCTAA TCCGGCAGGT CTCACCTGAT GACTGGAAAC GGTCTATTGT CGCCTACTTC 
AACAAACATG CGGGGAAGTC CAAGGAGGAA GCCAAGCTGG CCTTCCTCAA ACTCATCTTC 
AAGTGGCCCA CCTTTGGCTC AGCCTTCTTT GAGGTGAAGC AAACTACAGA ACCAAACTTC 
CCAGAGATTC TCTTAATTGC CATCAACAAG TACGGGGTCA GCCTCATCGA TCCCAGAACC 
AAGGACATCC TGACTACTCA CCCCTTCACC AAGATCTCCA ACTGGAGTAG TGGCAACACC 
TACTTCCACA TCACCATTGG GAACTTGGTC CGTGGGAGCA AACTGCTCTG TGAGACATCG 
CTGGGATACA AAATGGATGA TCTTCTGACT TCCTACATCA GCCAGATGCT CACAGCCATG 
AGCAAGCAGA GGAACTCCAG GAGTGGAAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241689.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239991.1 Mus musculus myosin VIIA (Myo7a), transcript variant X2, mRNA.

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CloneID	OMu68263
Clone ID Related Accession (Same CDS sequence)	XM_011241689.2 , XM_011241689.1
Accession Version	XM_011241689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGAGT GCCGCGTCTT GCTCTCACTG 
GGCTGCTCTG ACTTGGGCTG TGCTACTTGT CAATCGGGCC GGGCAGGGCT GACCCCGGCT 
GGACCCTGTT CTCCGTGTTG GTCCTGTAGG GGAACAAAGA TGATGGCCCC CAGCTTTACC 
CTGGCCACCA TCAAAGGAGA TGAGTACACC TTCACATCCA GCAATGCTGA GGACATCCGT 
GACCTGGTGG TCACCTTTCT GGAGGGGCTA CGGAAGAGGT CTAAGTATGT GGTGGCACTG 
CAGGACAATC CTAACCCTGC TGGTGAGGAG TCAGGCTTCC TCAGCTTCGC CAAGGGAGAC 
CTCATCATCC TTGACCATGA TACTGGTGAG CAGGTCATGA ACTCAGGCTG GGCCAACGGC 
ATCAACGAGA GGACCAAGCA GCGCGGCGAC TTCCCCACTG ACTGTGTATA CGTCATGCCC 
ACTGTCACCT TGCCACCAAG GGAGATTGTG GCCCTGGTCA CTATGACCCC AGACCAGAGG 
CAGGATGTCG TCCGGCTCCT GCAGCTTCGC ACAGCAGAGC CAGAGGTGCG CGCCAAGCCC 
TACACGCTAG AGGAGTTCTC CTACGACTAC TTCAGGCCCC CACCCAAGCA CACGCTGAGC 
CGTGTCATGG TGTCCAAGGC CCGCGGTAAG GACAGGCTGT GGAGCCACAC ACGAGAGCCC 
CTCAAGCAGG CCCTGCTCAA GAAGATCCTG GGCAGTGAAG AACTCTCCCA GGAAGCCTGC 
ATGGCCTTTG TAGCTGTGCT CAAGTACATG GGCGACTACC CATCCAAGAG GATGCGATCC 
GTCAATGAGC TCACTGACCA GATCTTTGAG TGGGCACTCA AGGCTGAGCC CCTCAAGGAT 
GAGGCCTACG TGCAGATCCT GAAGCAGCTG ACTGACAATC ACATCAGGTA CAGCGAAGAG 
AGGGGCTGGG AACTGCTGTG GCTGTGCACG GGCCTCTTCC CGCCCAGCAA CATCCTCCTG 
CCTCATGTTC AGCGGTTTCT GCAGTCCCGC AAGCACTGTC CTCTTGCCAT TGACTGCCTG 
CAGAGGCTCC AGAAAGCCCT GAGAAATGGC TCCCGGAAGT ACCCTCCGCA CCTGGTGGAG 
GTGGAGGCCA TCCAACATAA GACTACCCAG ATCTTCCACA AGGTCTACTT CCCCGATGAC 
ACGGACGAGG CTTTTGAGGT GGAGTCCAGC ACCAAGGCCA AGGACTTCTG CCAGAACATC 
GCCAGCCGGC TGCTGCTCAA GTCTTCCGAG GGATTCAGCC TTTTTGTCAA AATCGCAGAT 
AAGGTCATCA GCGTCCCAGA GAATGATTTC TTCTTTGACT TTGTCCGACA CCTGACAGAC 
TGGATAAAGA AAGCACGGCC CATCAAGGAC GGAATCGTGC CCTCACTAAC CTACCAGGTG 
TTCTTCATGA AGAAGCTGTG GACCACCACA GTGCCGGGCA AGGACCCCAT GGCTGACTCC 
ATCTTCCACT ATTACCAGGA ACTGCCCAAA TATCTCCGAG GCTACCACAA GTGCACCCGG 
GAGGAGGTGC TGCAGCTGGG CGCACTCATC TACAGGGTCA AGTTTGAGGA GGACAAATCC 
TACTTCCCTA GCATCCCCAA GTTGCTGAGG GAGCTGGTAC CCCAGGACCT AATCCGGCAG 
GTCTCACCTG ATGACTGGAA ACGGTCTATT GTCGCCTACT TCAACAAACA TGCGGGGAAG 
TCCAAGGAGG AAGCCAAGCT GGCCTTCCTC AAACTCATCT TCAAGTGGCC CACCTTTGGC 
TCAGCCTTCT TTGAGGTGAA GCAAACTACA GAACCAAACT TCCCAGAGAT TCTCTTAATT 
GCCATCAACA AGTACGGGGT CAGCCTCATC GATCCCAGAA CCAAGGACAT CCTGACTACT 
CACCCCTTCA CCAAGATCTC CAACTGGAGT AGTGGCAACA CCTACTTCCA CATCACCATT 
GGGAACTTGG TCCGTGGGAG CAAACTGCTC TGTGAGACAT CGCTGGGATA CAAAATGGAT 
GATCTTCTGA CTTCCTACAT CAGCCAGATG CTCACAGCCA TGAGCAAGCA GAGGAACTCC 
AGGAGTGGAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239991.1
CDS	34..6648
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241689.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241689.2

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGAGT GCCGCGTCTT GCTCTCACTG 
GGCTGCTCTG ACTTGGGCTG TGCTACTTGT CAATCGGGCC GGGCAGGGCT GACCCCGGCT 
GGACCCTGTT CTCCGTGTTG GTCCTGTAGG GGAACAAAGA TGATGGCCCC CAGCTTTACC 
CTGGCCACCA TCAAAGGAGA TGAGTACACC TTCACATCCA GCAATGCTGA GGACATCCGT 
GACCTGGTGG TCACCTTTCT GGAGGGGCTA CGGAAGAGGT CTAAGTATGT GGTGGCACTG 
CAGGACAATC CTAACCCTGC TGGTGAGGAG TCAGGCTTCC TCAGCTTCGC CAAGGGAGAC 
CTCATCATCC TTGACCATGA TACTGGTGAG CAGGTCATGA ACTCAGGCTG GGCCAACGGC 
ATCAACGAGA GGACCAAGCA GCGCGGCGAC TTCCCCACTG ACTGTGTATA CGTCATGCCC 
ACTGTCACCT TGCCACCAAG GGAGATTGTG GCCCTGGTCA CTATGACCCC AGACCAGAGG 
CAGGATGTCG TCCGGCTCCT GCAGCTTCGC ACAGCAGAGC CAGAGGTGCG CGCCAAGCCC 
TACACGCTAG AGGAGTTCTC CTACGACTAC TTCAGGCCCC CACCCAAGCA CACGCTGAGC 
CGTGTCATGG TGTCCAAGGC CCGCGGTAAG GACAGGCTGT GGAGCCACAC ACGAGAGCCC 
CTCAAGCAGG CCCTGCTCAA GAAGATCCTG GGCAGTGAAG AACTCTCCCA GGAAGCCTGC 
ATGGCCTTTG TAGCTGTGCT CAAGTACATG GGCGACTACC CATCCAAGAG GATGCGATCC 
GTCAATGAGC TCACTGACCA GATCTTTGAG TGGGCACTCA AGGCTGAGCC CCTCAAGGAT 
GAGGCCTACG TGCAGATCCT GAAGCAGCTG ACTGACAATC ACATCAGGTA CAGCGAAGAG 
AGGGGCTGGG AACTGCTGTG GCTGTGCACG GGCCTCTTCC CGCCCAGCAA CATCCTCCTG 
CCTCATGTTC AGCGGTTTCT GCAGTCCCGC AAGCACTGTC CTCTTGCCAT TGACTGCCTG 
CAGAGGCTCC AGAAAGCCCT GAGAAATGGC TCCCGGAAGT ACCCTCCGCA CCTGGTGGAG 
GTGGAGGCCA TCCAACATAA GACTACCCAG ATCTTCCACA AGGTCTACTT CCCCGATGAC 
ACGGACGAGG CTTTTGAGGT GGAGTCCAGC ACCAAGGCCA AGGACTTCTG CCAGAACATC 
GCCAGCCGGC TGCTGCTCAA GTCTTCCGAG GGATTCAGCC TTTTTGTCAA AATCGCAGAT 
AAGGTCATCA GCGTCCCAGA GAATGATTTC TTCTTTGACT TTGTCCGACA CCTGACAGAC 
TGGATAAAGA AAGCACGGCC CATCAAGGAC GGAATCGTGC CCTCACTAAC CTACCAGGTG 
TTCTTCATGA AGAAGCTGTG GACCACCACA GTGCCGGGCA AGGACCCCAT GGCTGACTCC 
ATCTTCCACT ATTACCAGGA ACTGCCCAAA TATCTCCGAG GCTACCACAA GTGCACCCGG 
GAGGAGGTGC TGCAGCTGGG CGCACTCATC TACAGGGTCA AGTTTGAGGA GGACAAATCC 
TACTTCCCTA GCATCCCCAA GTTGCTGAGG GAGCTGGTAC CCCAGGACCT AATCCGGCAG 
GTCTCACCTG ATGACTGGAA ACGGTCTATT GTCGCCTACT TCAACAAACA TGCGGGGAAG 
TCCAAGGAGG AAGCCAAGCT GGCCTTCCTC AAACTCATCT TCAAGTGGCC CACCTTTGGC 
TCAGCCTTCT TTGAGGTGAA GCAAACTACA GAACCAAACT TCCCAGAGAT TCTCTTAATT 
GCCATCAACA AGTACGGGGT CAGCCTCATC GATCCCAGAA CCAAGGACAT CCTGACTACT 
CACCCCTTCA CCAAGATCTC CAACTGGAGT AGTGGCAACA CCTACTTCCA CATCACCATT 
GGGAACTTGG TCCGTGGGAG CAAACTGCTC TGTGAGACAT CGCTGGGATA CAAAATGGAT 
GATCTTCTGA CTTCCTACAT CAGCCAGATG CTCACAGCCA TGAGCAAGCA GAGGAACTCC 
AGGAGTGGAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241689.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239991.1 Mus musculus myosin VIIA (Myo7a), transcript variant X2, mRNA.

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CloneID	OMu68263
Clone ID Related Accession (Same CDS sequence)	XM_011241689.2 , XM_011241689.1
Accession Version	XM_011241689.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGAGT GCCGCGTCTT GCTCTCACTG 
GGCTGCTCTG ACTTGGGCTG TGCTACTTGT CAATCGGGCC GGGCAGGGCT GACCCCGGCT 
GGACCCTGTT CTCCGTGTTG GTCCTGTAGG GGAACAAAGA TGATGGCCCC CAGCTTTACC 
CTGGCCACCA TCAAAGGAGA TGAGTACACC TTCACATCCA GCAATGCTGA GGACATCCGT 
GACCTGGTGG TCACCTTTCT GGAGGGGCTA CGGAAGAGGT CTAAGTATGT GGTGGCACTG 
CAGGACAATC CTAACCCTGC TGGTGAGGAG TCAGGCTTCC TCAGCTTCGC CAAGGGAGAC 
CTCATCATCC TTGACCATGA TACTGGTGAG CAGGTCATGA ACTCAGGCTG GGCCAACGGC 
ATCAACGAGA GGACCAAGCA GCGCGGCGAC TTCCCCACTG ACTGTGTATA CGTCATGCCC 
ACTGTCACCT TGCCACCAAG GGAGATTGTG GCCCTGGTCA CTATGACCCC AGACCAGAGG 
CAGGATGTCG TCCGGCTCCT GCAGCTTCGC ACAGCAGAGC CAGAGGTGCG CGCCAAGCCC 
TACACGCTAG AGGAGTTCTC CTACGACTAC TTCAGGCCCC CACCCAAGCA CACGCTGAGC 
CGTGTCATGG TGTCCAAGGC CCGCGGTAAG GACAGGCTGT GGAGCCACAC ACGAGAGCCC 
CTCAAGCAGG CCCTGCTCAA GAAGATCCTG GGCAGTGAAG AACTCTCCCA GGAAGCCTGC 
ATGGCCTTTG TAGCTGTGCT CAAGTACATG GGCGACTACC CATCCAAGAG GATGCGATCC 
GTCAATGAGC TCACTGACCA GATCTTTGAG TGGGCACTCA AGGCTGAGCC CCTCAAGGAT 
GAGGCCTACG TGCAGATCCT GAAGCAGCTG ACTGACAATC ACATCAGGTA CAGCGAAGAG 
AGGGGCTGGG AACTGCTGTG GCTGTGCACG GGCCTCTTCC CGCCCAGCAA CATCCTCCTG 
CCTCATGTTC AGCGGTTTCT GCAGTCCCGC AAGCACTGTC CTCTTGCCAT TGACTGCCTG 
CAGAGGCTCC AGAAAGCCCT GAGAAATGGC TCCCGGAAGT ACCCTCCGCA CCTGGTGGAG 
GTGGAGGCCA TCCAACATAA GACTACCCAG ATCTTCCACA AGGTCTACTT CCCCGATGAC 
ACGGACGAGG CTTTTGAGGT GGAGTCCAGC ACCAAGGCCA AGGACTTCTG CCAGAACATC 
GCCAGCCGGC TGCTGCTCAA GTCTTCCGAG GGATTCAGCC TTTTTGTCAA AATCGCAGAT 
AAGGTCATCA GCGTCCCAGA GAATGATTTC TTCTTTGACT TTGTCCGACA CCTGACAGAC 
TGGATAAAGA AAGCACGGCC CATCAAGGAC GGAATCGTGC CCTCACTAAC CTACCAGGTG 
TTCTTCATGA AGAAGCTGTG GACCACCACA GTGCCGGGCA AGGACCCCAT GGCTGACTCC 
ATCTTCCACT ATTACCAGGA ACTGCCCAAA TATCTCCGAG GCTACCACAA GTGCACCCGG 
GAGGAGGTGC TGCAGCTGGG CGCACTCATC TACAGGGTCA AGTTTGAGGA GGACAAATCC 
TACTTCCCTA GCATCCCCAA GTTGCTGAGG GAGCTGGTAC CCCAGGACCT AATCCGGCAG 
GTCTCACCTG ATGACTGGAA ACGGTCTATT GTCGCCTACT TCAACAAACA TGCGGGGAAG 
TCCAAGGAGG AAGCCAAGCT GGCCTTCCTC AAACTCATCT TCAAGTGGCC CACCTTTGGC 
TCAGCCTTCT TTGAGGTGAA GCAAACTACA GAACCAAACT TCCCAGAGAT TCTCTTAATT 
GCCATCAACA AGTACGGGGT CAGCCTCATC GATCCCAGAA CCAAGGACAT CCTGACTACT 
CACCCCTTCA CCAAGATCTC CAACTGGAGT AGTGGCAACA CCTACTTCCA CATCACCATT 
GGGAACTTGG TCCGTGGGAG CAAACTGCTC TGTGAGACAT CGCTGGGATA CAAAATGGAT 
GATCTTCTGA CTTCCTACAT CAGCCAGATG CTCACAGCCA TGAGCAAGCA GAGGAACTCC 
AGGAGTGGAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239991.1
CDS	35..6649
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241689.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241689.1

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGAGT GCCGCGTCTT GCTCTCACTG 
GGCTGCTCTG ACTTGGGCTG TGCTACTTGT CAATCGGGCC GGGCAGGGCT GACCCCGGCT 
GGACCCTGTT CTCCGTGTTG GTCCTGTAGG GGAACAAAGA TGATGGCCCC CAGCTTTACC 
CTGGCCACCA TCAAAGGAGA TGAGTACACC TTCACATCCA GCAATGCTGA GGACATCCGT 
GACCTGGTGG TCACCTTTCT GGAGGGGCTA CGGAAGAGGT CTAAGTATGT GGTGGCACTG 
CAGGACAATC CTAACCCTGC TGGTGAGGAG TCAGGCTTCC TCAGCTTCGC CAAGGGAGAC 
CTCATCATCC TTGACCATGA TACTGGTGAG CAGGTCATGA ACTCAGGCTG GGCCAACGGC 
ATCAACGAGA GGACCAAGCA GCGCGGCGAC TTCCCCACTG ACTGTGTATA CGTCATGCCC 
ACTGTCACCT TGCCACCAAG GGAGATTGTG GCCCTGGTCA CTATGACCCC AGACCAGAGG 
CAGGATGTCG TCCGGCTCCT GCAGCTTCGC ACAGCAGAGC CAGAGGTGCG CGCCAAGCCC 
TACACGCTAG AGGAGTTCTC CTACGACTAC TTCAGGCCCC CACCCAAGCA CACGCTGAGC 
CGTGTCATGG TGTCCAAGGC CCGCGGTAAG GACAGGCTGT GGAGCCACAC ACGAGAGCCC 
CTCAAGCAGG CCCTGCTCAA GAAGATCCTG GGCAGTGAAG AACTCTCCCA GGAAGCCTGC 
ATGGCCTTTG TAGCTGTGCT CAAGTACATG GGCGACTACC CATCCAAGAG GATGCGATCC 
GTCAATGAGC TCACTGACCA GATCTTTGAG TGGGCACTCA AGGCTGAGCC CCTCAAGGAT 
GAGGCCTACG TGCAGATCCT GAAGCAGCTG ACTGACAATC ACATCAGGTA CAGCGAAGAG 
AGGGGCTGGG AACTGCTGTG GCTGTGCACG GGCCTCTTCC CGCCCAGCAA CATCCTCCTG 
CCTCATGTTC AGCGGTTTCT GCAGTCCCGC AAGCACTGTC CTCTTGCCAT TGACTGCCTG 
CAGAGGCTCC AGAAAGCCCT GAGAAATGGC TCCCGGAAGT ACCCTCCGCA CCTGGTGGAG 
GTGGAGGCCA TCCAACATAA GACTACCCAG ATCTTCCACA AGGTCTACTT CCCCGATGAC 
ACGGACGAGG CTTTTGAGGT GGAGTCCAGC ACCAAGGCCA AGGACTTCTG CCAGAACATC 
GCCAGCCGGC TGCTGCTCAA GTCTTCCGAG GGATTCAGCC TTTTTGTCAA AATCGCAGAT 
AAGGTCATCA GCGTCCCAGA GAATGATTTC TTCTTTGACT TTGTCCGACA CCTGACAGAC 
TGGATAAAGA AAGCACGGCC CATCAAGGAC GGAATCGTGC CCTCACTAAC CTACCAGGTG 
TTCTTCATGA AGAAGCTGTG GACCACCACA GTGCCGGGCA AGGACCCCAT GGCTGACTCC 
ATCTTCCACT ATTACCAGGA ACTGCCCAAA TATCTCCGAG GCTACCACAA GTGCACCCGG 
GAGGAGGTGC TGCAGCTGGG CGCACTCATC TACAGGGTCA AGTTTGAGGA GGACAAATCC 
TACTTCCCTA GCATCCCCAA GTTGCTGAGG GAGCTGGTAC CCCAGGACCT AATCCGGCAG 
GTCTCACCTG ATGACTGGAA ACGGTCTATT GTCGCCTACT TCAACAAACA TGCGGGGAAG 
TCCAAGGAGG AAGCCAAGCT GGCCTTCCTC AAACTCATCT TCAAGTGGCC CACCTTTGGC 
TCAGCCTTCT TTGAGGTGAA GCAAACTACA GAACCAAACT TCCCAGAGAT TCTCTTAATT 
GCCATCAACA AGTACGGGGT CAGCCTCATC GATCCCAGAA CCAAGGACAT CCTGACTACT 
CACCCCTTCA CCAAGATCTC CAACTGGAGT AGTGGCAACA CCTACTTCCA CATCACCATT 
GGGAACTTGG TCCGTGGGAG CAAACTGCTC TGTGAGACAT CGCTGGGATA CAAAATGGAT 
GATCTTCTGA CTTCCTACAT CAGCCAGATG CTCACAGCCA TGAGCAAGCA GAGGAACTCC 
AGGAGTGGAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( NM_001256082.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243011.1 Mus musculus myosin VIIA (Myo7a), transcript variant 3, mRNA.

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CloneID	OMu20144
Clone ID Related Accession (Same CDS sequence)	XM_011241690.1 , XM_011241690.2 , NM_001256082.1
Accession Version	NM_001256082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115022.11, AY821854.1 and AC157792.4. Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY821854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGGAACA 
AAGATGATGG CCCCCAGCTT TACCCTGGCC ACCATCAAAG GAGATGAGTA CACCTTCACA 
TCCAGCAATG CTGAGGACAT CCGTGACCTG GTGGTCACCT TTCTGGAGGG GCTACGGAAG 
AGGTCTAAGT ATGTGGTGGC ACTGCAGGAC AATCCTAACC CTGCTGGTGA GGAGTCAGGC 
TTCCTCAGCT TCGCCAAGGG AGACCTCATC ATCCTTGACC ATGATACTGG TGAGCAGGTC 
ATGAACTCAG GCTGGGCCAA CGGCATCAAC GAGAGGACCA AGCAGCGCGG CGACTTCCCC 
ACTGACTGTG TATACGTCAT GCCCACTGTC ACCTTGCCAC CAAGGGAGAT TGTGGCCCTG 
GTCACTATGA CCCCAGACCA GAGGCAGGAT GTCGTCCGGC TCCTGCAGCT TCGCACAGCA 
GAGCCAGAGG TGCGCGCCAA GCCCTACACG CTAGAGGAGT TCTCCTACGA CTACTTCAGG 
CCCCCACCCA AGCACACGCT GAGCCGTGTC ATGGTGTCCA AGGCCCGCGG TAAGGACAGG 
CTGTGGAGCC ACACACGAGA GCCCCTCAAG CAGGCCCTGC TCAAGAAGAT CCTGGGCAGT 
GAAGAACTCT CCCAGGAAGC CTGCATGGCC TTTGTAGCTG TGCTCAAGTA CATGGGCGAC 
TACCCATCCA AGAGGATGCG ATCCGTCAAT GAGCTCACTG ACCAGATCTT TGAGTGGGCA 
CTCAAGGCTG AGCCCCTCAA GGATGAGGCC TACGTGCAGA TCCTGAAGCA GCTGACTGAC 
AATCACATCA GGTACAGCGA AGAGAGGGGC TGGGAACTGC TGTGGCTGTG CACGGGCCTC 
TTCCCGCCCA GCAACATCCT CCTGCCTCAT GTTCAGCGGT TTCTGCAGTC CCGCAAGCAC 
TGTCCTCTTG CCATTGACTG CCTGCAGAGG CTCCAGAAAG CCCTGAGAAA TGGCTCCCGG 
AAGTACCCTC CGCACCTGGT GGAGGTGGAG GCCATCCAAC ATAAGACTAC CCAGATCTTC 
CACAAGGTCT ACTTCCCCGA TGACACGGAC GAGGCTTTTG AGGTGGAGTC CAGCACCAAG 
GCCAAGGACT TCTGCCAGAA CATCGCCAGC CGGCTGCTGC TCAAGTCTTC CGAGGGATTC 
AGCCTTTTTG TCAAAATCGC AGATAAGGTC ATCAGCGTCC CAGAGAATGA TTTCTTCTTT 
GACTTTGTCC GACACCTGAC AGACTGGATA AAGAAAGCAC GGCCCATCAA GGACGGAATC 
GTGCCCTCAC TAACCTACCA GGTGTTCTTC ATGAAGAAGC TGTGGACCAC CACAGTGCCG 
GGCAAGGACC CCATGGCTGA CTCCATCTTC CACTATTACC AGGAACTGCC CAAATATCTC 
CGAGGCTACC ACAAGTGCAC CCGGGAGGAG GTGCTGCAGC TGGGCGCACT CATCTACAGG 
GTCAAGTTTG AGGAGGACAA ATCCTACTTC CCTAGCATCC CCAAGTTGCT GAGGGAGCTG 
GTACCCCAGG ACCTAATCCG GCAGGTCTCA CCTGATGACT GGAAACGGTC TATTGTCGCC 
TACTTCAACA AACATGCGGG GAAGTCCAAG GAGGAAGCCA AGCTGGCCTT CCTCAAACTC 
ATCTTCAAGT GGCCCACCTT TGGCTCAGCC TTCTTTGAGG TGAAGCAAAC TACAGAACCA 
AACTTCCCAG AGATTCTCTT AATTGCCATC AACAAGTACG GGGTCAGCCT CATCGATCCC 
AGAACCAAGG ACATCCTGAC TACTCACCCC TTCACCAAGA TCTCCAACTG GAGTAGTGGC 
AACACCTACT TCCACATCAC CATTGGGAAC TTGGTCCGTG GGAGCAAACT GCTCTGTGAG 
ACATCGCTGG GATACAAAAT GGATGATCTT CTGACTTCCT ACATCAGCCA GATGCTCACA 
GCCATGAGCA AGCAGAGGAA CTCCAGGAGT GGAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243011.1
CDS	123..6641
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	NM_001256082.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256082.1

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGGAACA 
AAGATGATGG CCCCCAGCTT TACCCTGGCC ACCATCAAAG GAGATGAGTA CACCTTCACA 
TCCAGCAATG CTGAGGACAT CCGTGACCTG GTGGTCACCT TTCTGGAGGG GCTACGGAAG 
AGGTCTAAGT ATGTGGTGGC ACTGCAGGAC AATCCTAACC CTGCTGGTGA GGAGTCAGGC 
TTCCTCAGCT TCGCCAAGGG AGACCTCATC ATCCTTGACC ATGATACTGG TGAGCAGGTC 
ATGAACTCAG GCTGGGCCAA CGGCATCAAC GAGAGGACCA AGCAGCGCGG CGACTTCCCC 
ACTGACTGTG TATACGTCAT GCCCACTGTC ACCTTGCCAC CAAGGGAGAT TGTGGCCCTG 
GTCACTATGA CCCCAGACCA GAGGCAGGAT GTCGTCCGGC TCCTGCAGCT TCGCACAGCA 
GAGCCAGAGG TGCGCGCCAA GCCCTACACG CTAGAGGAGT TCTCCTACGA CTACTTCAGG 
CCCCCACCCA AGCACACGCT GAGCCGTGTC ATGGTGTCCA AGGCCCGCGG TAAGGACAGG 
CTGTGGAGCC ACACACGAGA GCCCCTCAAG CAGGCCCTGC TCAAGAAGAT CCTGGGCAGT 
GAAGAACTCT CCCAGGAAGC CTGCATGGCC TTTGTAGCTG TGCTCAAGTA CATGGGCGAC 
TACCCATCCA AGAGGATGCG ATCCGTCAAT GAGCTCACTG ACCAGATCTT TGAGTGGGCA 
CTCAAGGCTG AGCCCCTCAA GGATGAGGCC TACGTGCAGA TCCTGAAGCA GCTGACTGAC 
AATCACATCA GGTACAGCGA AGAGAGGGGC TGGGAACTGC TGTGGCTGTG CACGGGCCTC 
TTCCCGCCCA GCAACATCCT CCTGCCTCAT GTTCAGCGGT TTCTGCAGTC CCGCAAGCAC 
TGTCCTCTTG CCATTGACTG CCTGCAGAGG CTCCAGAAAG CCCTGAGAAA TGGCTCCCGG 
AAGTACCCTC CGCACCTGGT GGAGGTGGAG GCCATCCAAC ATAAGACTAC CCAGATCTTC 
CACAAGGTCT ACTTCCCCGA TGACACGGAC GAGGCTTTTG AGGTGGAGTC CAGCACCAAG 
GCCAAGGACT TCTGCCAGAA CATCGCCAGC CGGCTGCTGC TCAAGTCTTC CGAGGGATTC 
AGCCTTTTTG TCAAAATCGC AGATAAGGTC ATCAGCGTCC CAGAGAATGA TTTCTTCTTT 
GACTTTGTCC GACACCTGAC AGACTGGATA AAGAAAGCAC GGCCCATCAA GGACGGAATC 
GTGCCCTCAC TAACCTACCA GGTGTTCTTC ATGAAGAAGC TGTGGACCAC CACAGTGCCG 
GGCAAGGACC CCATGGCTGA CTCCATCTTC CACTATTACC AGGAACTGCC CAAATATCTC 
CGAGGCTACC ACAAGTGCAC CCGGGAGGAG GTGCTGCAGC TGGGCGCACT CATCTACAGG 
GTCAAGTTTG AGGAGGACAA ATCCTACTTC CCTAGCATCC CCAAGTTGCT GAGGGAGCTG 
GTACCCCAGG ACCTAATCCG GCAGGTCTCA CCTGATGACT GGAAACGGTC TATTGTCGCC 
TACTTCAACA AACATGCGGG GAAGTCCAAG GAGGAAGCCA AGCTGGCCTT CCTCAAACTC 
ATCTTCAAGT GGCCCACCTT TGGCTCAGCC TTCTTTGAGG TGAAGCAAAC TACAGAACCA 
AACTTCCCAG AGATTCTCTT AATTGCCATC AACAAGTACG GGGTCAGCCT CATCGATCCC 
AGAACCAAGG ACATCCTGAC TACTCACCCC TTCACCAAGA TCTCCAACTG GAGTAGTGGC 
AACACCTACT TCCACATCAC CATTGGGAAC TTGGTCCGTG GGAGCAAACT GCTCTGTGAG 
ACATCGCTGG GATACAAAAT GGATGATCTT CTGACTTCCT ACATCAGCCA GATGCTCACA 
GCCATGAGCA AGCAGAGGAA CTCCAGGAGT GGAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( NM_008663.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032689.2 Mus musculus myosin VIIA (Myo7a), transcript variant 2, mRNA.

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CloneID	OMu20162
Clone ID Related Accession (Same CDS sequence)	NM_008663.2
Accession Version	NM_008663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115022.11, AY821853.1 and AC157792.4. On Oct 4, 2006 this sequence version replaced NM_008663.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACGTA CACAGGTTCC 
ATCCTGGTGG CCGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTACACCA ACAAGAAGAT AGGGGAGATG CCCCCCCACA TCTTCGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAACCGG GACCAGTGCT GTATTATCAG CGGGGAGTCG 
GGAGCTGGCA AGACAGAGAG CACAAAGTTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGA 
CAGCACTCAT GGATCGAGCA GCAGGTGCTG GAGGCCACCC CGATCCTGGA AGCATTTGGG 
AACGCCAAGA CCATCCGCAA CGACAACTCT AGCCGCTTTG GCAAGTACAT TGACATCCAC 
TTTAACAAGC GTGGTGCCAT CGAGGGCGCC AAAATAGAGC AATACCTGCT GGAGAAGTCA 
CGTGTCTGCC GCCAGGCCCC TGACGAGAGG AACTATCACG TGTTCTACTG TATGCTGGAG 
GGCATGAATG AGGAGGAGAA GAAGAAACTG GGCCTAGGCC AGGCCGCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT CACCTGTGAG GGCCGCGTGG ACAGTCAGGA GTATGCCAAC 
ATCCGCTCTG CCATGAAGGT TCTCATGTTC ACAGACACGG AGAACTGGGA GATCTCGAAG 
CTTCTGGCTG CCATCCTACA CATGGGCAAT CTGCAGTATG AGGCCCGGAC ATTTGAGAAC 
TTGGATGCGT GTGAAGTCCT CTTCTCCCCA TCGCTGGCCA CGGCAGCTTC TCTGCTCGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTC ACCAGCCGCA CCCTCATCAC CCGTGGGGAG 
ACGGTGTCCA CCCCTCTCAG CAGGGAACAG GCGCTGGATG TGCGAGATGC CTTTGTCAAG 
GGCATCTATG GGCGGCTCTT TGTGTGGATT GTGGAGAAGA TCAACGCAGC AATCTACAAG 
CCACCCCCCC TGGAAGTGAA GAACTCTCGC CGGTCCATCG GTCTCCTGGA CATCTTTGGA 
TTTGAGAACT TCACTGTGAA CAGCTTCGAG CAGCTCTGCA TTAACTTTGC CAATGAGCAC 
CTGCAGCAAT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA CGACCTGGAG 
AGCATCGACT GGTTGCACAT TGAGTTCACT GACAACCAGG AAGCACTGGA CATGATTGCC 
AACCGGCCTA TGAACGTCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG 
GATGCCACCA TGCTGCATAA GCTGAACTCA CAGCACAAGC TCAATGCCAA CTACGTGCCA 
CCCAAGAACA GCCACGAGAC CCAGTTTGGA ATCAACCACT TTGCGGGTGT TGTCTATTAT 
GAGAGTCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT CATCCAGCTG 
GTCCACTCTT CCCGGAACAA GTTCATAAAG CAGATTTTCC AAGCTGACGT TGCCATGGGT 
GCCGAGACCA GGAAGCGCTC GCCTACACTC AGCAGCCAGT TCAAGCGGTC TCTGGAGCTG 
CTGATGCGCA CACTGGGCGC CTGCCAGCCC TTCTTTGTGC GTTGTATCAA ACCCAATGAG 
TTCAAGAAGC CCATGCTCTT CGACCGGCAC TTGTGTGTAC GCCAGCTGCG ATATTCGGGC 
ATGATGGAGA CAATCCGCAT CCGCCACGCA GGCTACCCCA TTCGCTACAG CTTTGTGGAG 
TTTGTGGAGC GCTACCGGGT ACTGCTGCCT GGTGTGAAGC CAGCATACAA GCAGGGTGAC 
CTCCGAGGGA CATGCCAGCG CATGGCTGAG GCTGTGCTGG GCACGCACGA TGACTGGCAG 
ATTGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGTTGCTGGA GGTGGAGCGG 
GACAAGGCCA TCACAGACAG AGTCATTCTC CTCCAGAAGG TTATCCGGGG CTTCAAAGAC 
AGGTCCAACT TCCTGAGACT GAAGAGTGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC 
CACCACTGTA GGAAAAACTA TGAGCTGATT CGTCTTGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CAAGCAGTAC CGCCTGGCCA GACAGCGCAT AATAGAGTTC 
CAGGCCCGCT GCCGGGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG 
ATCACCGTGC AGGCCTATGC CCGAGGCATG ATTGCCCGCC GGCTACACCG CCGCCTCCGG 
GTTGAGTACC AGCGGCGCCT CGAGGCAGAG AGGATGCGTC TGGCAGAGGA GGAGAAACTC 
CGAAAGGAGA TGAGTGCCAA GAAGGCCAAA GAGGAGGCTG AGCGCAAGCA TCAGGAGCGC 
CTGGCTCAGC TAGCCCGCGA GGATGCGGAG CGGGAACTGA AGGAGAAGGA GGAGGCTCGG 
AGGAAGAAGG AACTGCTGGA GCAGATGGAG AAGGCCCGCC ACGAACCCAT CAACCACTCA 
GATATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GCAGCCTGCC AGGCCAGGAA 
GGCCAGGCGC CTAGTGGCTT TGAGGACCTA GAGCGCGGAC GGAGGGAGAT GGTGGAAGAG 
GATGTTGACG CTGCCCTGCC CCTGCCTGAT GAAGACGAGG AGGACCTTTC TGAGTACAAA 
TTCGCCAAGT TTGCTGCCAC CTACTTCCAG GGCACAACCA CACACTCCTA CACCCGGAGG 
CCTCTCAAGC AGCCGCTGCT CTACCACGAC GATGAGGGTG ACCAGCTGGC GGCGCTGGCT 
GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAAGTA CCACACAGCC 
ATGAGCGACG GCAGTGAGAA GATCCCAGTG ATGACTAAGA TCTACGAGAC CCTAGGCAAG 
AAGACATATA AGAGGGAGCT GCAGGCCTTG CAGGGCGAGG GCGAGACCCA GCTCCCTGAG 
GGGCAGAAGA AGACCAGTGT GAGACACAAG TTGGTACACT TGACACTGAA GAAAAAGTCC 
AAACTCACAG AAGAGGTGAC CAAGAGGCTG AACGATGGGG AATCCACGGT ACAGGGCAAC 
AGCATGCTGG AGGATCGGCC CACCTCAAAT CTAGAGAAGC TGCACTTCAT CATCGGCAAC 
GGCATCCTGC GGCCTGCGCT GCGGGACGAG ATTTACTGCC AGATCAGTAA GCAGCTCACA 
CACAACCCAT CCAAGAGCAG CTATGCCAGG GGCTGGATCC TCGTGTCGCT CTGTGTGGGC 
TGCTTCGCCC CCTCTGAGAA GTTCGTTAAG TACCTGCGGA ACTTCATCCA CGGAGGCCCA 
CCTGGCTATG CTCCTTACTG TGAGGAGCGC CTGAGGAGGA CCTTTGTCAA CGGAACTCGG 
ACACAGCCAC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG 
CCCGTGACCT TCATGGATGG GACCACCAAG ACCCTGCTAA CAGATTCAGC AACTACAGCC 
AGGGAGCTGT GCAATGCTCT GGCTGACAAG ATCTCACTCA AGGACCGCTT TGGCTTCTCC 
CTCTACATCG CTCTGTTCGA TAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCATGTCATG 
GATGCCATCT CTCAGTGTGA GCAGTACGCC AAGGAGCAGG GTGCTCAGGA GCGCAACGCC 
CCATGGAGGC TCTTCTTTAG AAAGGAGGTC TTCACACCCT GGCACAACCC CTCGGAGGAC 
AACGTGGCCA CGAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCTTCTC AGCAGTACTT TGTGGACTAT 
GGTTCTGAGA TGATTCTGGA GCGCCTGCTG AGCCTCGTGC CCACTTACAT CCCTGACCGT 
GAGATCACAC CGCTGAAGAA TCTTGAGAAG TGGGCACAGC TGGCCATTGC TGCCCACAAG 
AAGGGAATTT ATGCCCAGAG GAGAACTGAC TCCCAGAAGG TCAAAGAGGA TGTGGTCAAT 
TATGCCCGTT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ACGAAGCTTA CAAATTCTCA 
GGCCCTCCCC TCCCCAAGAG CGACGTCATC GTGGCTGTCA ACTGGACGGG TGTGTACTTC 
GTGGACGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CGGAGATCAT GGCTGTGTCC 
AGCAGTAGGG GAACAAAGAT GATGGCCCCC AGCTTTACCC TGGCCACCAT CAAAGGAGAT 
GAGTACACCT TCACATCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTTCTG 
GAGGGGCTAC GGAAGAGGTC TAAGTATGTG GTGGCACTGC AGGACAATCC TAACCCTGCT 
GGTGAGGAGT CAGGCTTCCT CAGCTTCGCC AAGGGAGACC TCATCATCCT TGACCATGAT 
ACTGGTGAGC AGGTCATGAA CTCAGGCTGG GCCAACGGCA TCAACGAGAG GACCAAGCAG 
CGCGGCGACT TCCCCACTGA CTGTGTATAC GTCATGCCCA CTGTCACCTT GCCACCAAGG 
GAGATTGTGG CCCTGGTCAC TATGACCCCA GACCAGAGGC AGGATGTCGT CCGGCTCCTG 
CAGCTTCGCA CAGCAGAGCC AGAGGTGCGC GCCAAGCCCT ACACGCTAGA GGAGTTCTCC 
TACGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGCGGTAAGG ACAGGCTGTG GAGCCACACA CGAGAGCCCC TCAAGCAGGC CCTGCTCAAG 
AAGATCCTGG GCAGTGAAGA ACTCTCCCAG GAAGCCTGCA TGGCCTTTGT AGCTGTGCTC 
AAGTACATGG GCGACTACCC ATCCAAGAGG ATGCGATCCG TCAATGAGCT CACTGACCAG 
ATCTTTGAGT GGGCACTCAA GGCTGAGCCC CTCAAGGATG AGGCCTACGT GCAGATCCTG 
AAGCAGCTGA CTGACAATCA CATCAGGTAC AGCGAAGAGA GGGGCTGGGA ACTGCTGTGG 
CTGTGCACGG GCCTCTTCCC GCCCAGCAAC ATCCTCCTGC CTCATGTTCA GCGGTTTCTG 
CAGTCCCGCA AGCACTGTCC TCTTGCCATT GACTGCCTGC AGAGGCTCCA GAAAGCCCTG 
AGAAATGGCT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAACATAAG 
ACTACCCAGA TCTTCCACAA GGTCTACTTC CCCGATGACA CGGACGAGGC TTTTGAGGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCAGCCGGCT GCTGCTCAAG 
TCTTCCGAGG GATTCAGCCT TTTTGTCAAA ATCGCAGATA AGGTCATCAG CGTCCCAGAG 
AATGATTTCT TCTTTGACTT TGTCCGACAC CTGACAGACT GGATAAAGAA AGCACGGCCC 
ATCAAGGACG GAATCGTGCC CTCACTAACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACAG TGCCGGGCAA GGACCCCATG GCTGACTCCA TCTTCCACTA TTACCAGGAA 
CTGCCCAAAT ATCTCCGAGG CTACCACAAG TGCACCCGGG AGGAGGTGCT GCAGCTGGGC 
GCACTCATCT ACAGGGTCAA GTTTGAGGAG GACAAATCCT ACTTCCCTAG CATCCCCAAG 
TTGCTGAGGG AGCTGGTACC CCAGGACCTA ATCCGGCAGG TCTCACCTGA TGACTGGAAA 
CGGTCTATTG TCGCCTACTT CAACAAACAT GCGGGGAAGT CCAAGGAGGA AGCCAAGCTG 
GCCTTCCTCA AACTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT TGAGGTGAAG 
CAAACTACAG AACCAAACTT CCCAGAGATT CTCTTAATTG CCATCAACAA GTACGGGGTC 
AGCCTCATCG ATCCCAGAAC CAAGGACATC CTGACTACTC ACCCCTTCAC CAAGATCTCC 
AACTGGAGTA GTGGCAACAC CTACTTCCAC ATCACCATTG GGAACTTGGT CCGTGGGAGC 
AAACTGCTCT GTGAGACATC GCTGGGATAC AAAATGGATG ATCTTCTGAC TTCCTACATC 
AGCCAGATGC TCACAGCCAT GAGCAAGCAG AGGAACTCCA GGAGTGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032689.2
CDS	261..6794
Translation	MVILQKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	NM_008663.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008663.2

ORF Insert Sequence:

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACGTA CACAGGTTCC 
ATCCTGGTGG CCGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTACACCA ACAAGAAGAT AGGGGAGATG CCCCCCCACA TCTTCGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAACCGG GACCAGTGCT GTATTATCAG CGGGGAGTCG 
GGAGCTGGCA AGACAGAGAG CACAAAGTTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGA 
CAGCACTCAT GGATCGAGCA GCAGGTGCTG GAGGCCACCC CGATCCTGGA AGCATTTGGG 
AACGCCAAGA CCATCCGCAA CGACAACTCT AGCCGCTTTG GCAAGTACAT TGACATCCAC 
TTTAACAAGC GTGGTGCCAT CGAGGGCGCC AAAATAGAGC AATACCTGCT GGAGAAGTCA 
CGTGTCTGCC GCCAGGCCCC TGACGAGAGG AACTATCACG TGTTCTACTG TATGCTGGAG 
GGCATGAATG AGGAGGAGAA GAAGAAACTG GGCCTAGGCC AGGCCGCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT CACCTGTGAG GGCCGCGTGG ACAGTCAGGA GTATGCCAAC 
ATCCGCTCTG CCATGAAGGT TCTCATGTTC ACAGACACGG AGAACTGGGA GATCTCGAAG 
CTTCTGGCTG CCATCCTACA CATGGGCAAT CTGCAGTATG AGGCCCGGAC ATTTGAGAAC 
TTGGATGCGT GTGAAGTCCT CTTCTCCCCA TCGCTGGCCA CGGCAGCTTC TCTGCTCGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTC ACCAGCCGCA CCCTCATCAC CCGTGGGGAG 
ACGGTGTCCA CCCCTCTCAG CAGGGAACAG GCGCTGGATG TGCGAGATGC CTTTGTCAAG 
GGCATCTATG GGCGGCTCTT TGTGTGGATT GTGGAGAAGA TCAACGCAGC AATCTACAAG 
CCACCCCCCC TGGAAGTGAA GAACTCTCGC CGGTCCATCG GTCTCCTGGA CATCTTTGGA 
TTTGAGAACT TCACTGTGAA CAGCTTCGAG CAGCTCTGCA TTAACTTTGC CAATGAGCAC 
CTGCAGCAAT TCTTCGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAGTA CGACCTGGAG 
AGCATCGACT GGTTGCACAT TGAGTTCACT GACAACCAGG AAGCACTGGA CATGATTGCC 
AACCGGCCTA TGAACGTCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACG 
GATGCCACCA TGCTGCATAA GCTGAACTCA CAGCACAAGC TCAATGCCAA CTACGTGCCA 
CCCAAGAACA GCCACGAGAC CCAGTTTGGA ATCAACCACT TTGCGGGTGT TGTCTATTAT 
GAGAGTCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT CATCCAGCTG 
GTCCACTCTT CCCGGAACAA GTTCATAAAG CAGATTTTCC AAGCTGACGT TGCCATGGGT 
GCCGAGACCA GGAAGCGCTC GCCTACACTC AGCAGCCAGT TCAAGCGGTC TCTGGAGCTG 
CTGATGCGCA CACTGGGCGC CTGCCAGCCC TTCTTTGTGC GTTGTATCAA ACCCAATGAG 
TTCAAGAAGC CCATGCTCTT CGACCGGCAC TTGTGTGTAC GCCAGCTGCG ATATTCGGGC 
ATGATGGAGA CAATCCGCAT CCGCCACGCA GGCTACCCCA TTCGCTACAG CTTTGTGGAG 
TTTGTGGAGC GCTACCGGGT ACTGCTGCCT GGTGTGAAGC CAGCATACAA GCAGGGTGAC 
CTCCGAGGGA CATGCCAGCG CATGGCTGAG GCTGTGCTGG GCACGCACGA TGACTGGCAG 
ATTGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGTTGCTGGA GGTGGAGCGG 
GACAAGGCCA TCACAGACAG AGTCATTCTC CTCCAGAAGG TTATCCGGGG CTTCAAAGAC 
AGGTCCAACT TCCTGAGACT GAAGAGTGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGC 
CACCACTGTA GGAAAAACTA TGAGCTGATT CGTCTTGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CAAGCAGTAC CGCCTGGCCA GACAGCGCAT AATAGAGTTC 
CAGGCCCGCT GCCGGGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCCGTG 
ATCACCGTGC AGGCCTATGC CCGAGGCATG ATTGCCCGCC GGCTACACCG CCGCCTCCGG 
GTTGAGTACC AGCGGCGCCT CGAGGCAGAG AGGATGCGTC TGGCAGAGGA GGAGAAACTC 
CGAAAGGAGA TGAGTGCCAA GAAGGCCAAA GAGGAGGCTG AGCGCAAGCA TCAGGAGCGC 
CTGGCTCAGC TAGCCCGCGA GGATGCGGAG CGGGAACTGA AGGAGAAGGA GGAGGCTCGG 
AGGAAGAAGG AACTGCTGGA GCAGATGGAG AAGGCCCGCC ACGAACCCAT CAACCACTCA 
GATATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GCAGCCTGCC AGGCCAGGAA 
GGCCAGGCGC CTAGTGGCTT TGAGGACCTA GAGCGCGGAC GGAGGGAGAT GGTGGAAGAG 
GATGTTGACG CTGCCCTGCC CCTGCCTGAT GAAGACGAGG AGGACCTTTC TGAGTACAAA 
TTCGCCAAGT TTGCTGCCAC CTACTTCCAG GGCACAACCA CACACTCCTA CACCCGGAGG 
CCTCTCAAGC AGCCGCTGCT CTACCACGAC GATGAGGGTG ACCAGCTGGC GGCGCTGGCT 
GTCTGGATCA CCATCCTCCG GTTCATGGGG GACCTCCCAG AGCCCAAGTA CCACACAGCC 
ATGAGCGACG GCAGTGAGAA GATCCCAGTG ATGACTAAGA TCTACGAGAC CCTAGGCAAG 
AAGACATATA AGAGGGAGCT GCAGGCCTTG CAGGGCGAGG GCGAGACCCA GCTCCCTGAG 
GGGCAGAAGA AGACCAGTGT GAGACACAAG TTGGTACACT TGACACTGAA GAAAAAGTCC 
AAACTCACAG AAGAGGTGAC CAAGAGGCTG AACGATGGGG AATCCACGGT ACAGGGCAAC 
AGCATGCTGG AGGATCGGCC CACCTCAAAT CTAGAGAAGC TGCACTTCAT CATCGGCAAC 
GGCATCCTGC GGCCTGCGCT GCGGGACGAG ATTTACTGCC AGATCAGTAA GCAGCTCACA 
CACAACCCAT CCAAGAGCAG CTATGCCAGG GGCTGGATCC TCGTGTCGCT CTGTGTGGGC 
TGCTTCGCCC CCTCTGAGAA GTTCGTTAAG TACCTGCGGA ACTTCATCCA CGGAGGCCCA 
CCTGGCTATG CTCCTTACTG TGAGGAGCGC CTGAGGAGGA CCTTTGTCAA CGGAACTCGG 
ACACAGCCAC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC CATCATGTTG 
CCCGTGACCT TCATGGATGG GACCACCAAG ACCCTGCTAA CAGATTCAGC AACTACAGCC 
AGGGAGCTGT GCAATGCTCT GGCTGACAAG ATCTCACTCA AGGACCGCTT TGGCTTCTCC 
CTCTACATCG CTCTGTTCGA TAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCATGTCATG 
GATGCCATCT CTCAGTGTGA GCAGTACGCC AAGGAGCAGG GTGCTCAGGA GCGCAACGCC 
CCATGGAGGC TCTTCTTTAG AAAGGAGGTC TTCACACCCT GGCACAACCC CTCGGAGGAC 
AACGTGGCCA CGAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCTTCTC AGCAGTACTT TGTGGACTAT 
GGTTCTGAGA TGATTCTGGA GCGCCTGCTG AGCCTCGTGC CCACTTACAT CCCTGACCGT 
GAGATCACAC CGCTGAAGAA TCTTGAGAAG TGGGCACAGC TGGCCATTGC TGCCCACAAG 
AAGGGAATTT ATGCCCAGAG GAGAACTGAC TCCCAGAAGG TCAAAGAGGA TGTGGTCAAT 
TATGCCCGTT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ACGAAGCTTA CAAATTCTCA 
GGCCCTCCCC TCCCCAAGAG CGACGTCATC GTGGCTGTCA ACTGGACGGG TGTGTACTTC 
GTGGACGAGC AGGAGCAGGT GCTTCTGGAG CTGTCCTTCC CGGAGATCAT GGCTGTGTCC 
AGCAGTAGGG GAACAAAGAT GATGGCCCCC AGCTTTACCC TGGCCACCAT CAAAGGAGAT 
GAGTACACCT TCACATCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTTCTG 
GAGGGGCTAC GGAAGAGGTC TAAGTATGTG GTGGCACTGC AGGACAATCC TAACCCTGCT 
GGTGAGGAGT CAGGCTTCCT CAGCTTCGCC AAGGGAGACC TCATCATCCT TGACCATGAT 
ACTGGTGAGC AGGTCATGAA CTCAGGCTGG GCCAACGGCA TCAACGAGAG GACCAAGCAG 
CGCGGCGACT TCCCCACTGA CTGTGTATAC GTCATGCCCA CTGTCACCTT GCCACCAAGG 
GAGATTGTGG CCCTGGTCAC TATGACCCCA GACCAGAGGC AGGATGTCGT CCGGCTCCTG 
CAGCTTCGCA CAGCAGAGCC AGAGGTGCGC GCCAAGCCCT ACACGCTAGA GGAGTTCTCC 
TACGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGCGGTAAGG ACAGGCTGTG GAGCCACACA CGAGAGCCCC TCAAGCAGGC CCTGCTCAAG 
AAGATCCTGG GCAGTGAAGA ACTCTCCCAG GAAGCCTGCA TGGCCTTTGT AGCTGTGCTC 
AAGTACATGG GCGACTACCC ATCCAAGAGG ATGCGATCCG TCAATGAGCT CACTGACCAG 
ATCTTTGAGT GGGCACTCAA GGCTGAGCCC CTCAAGGATG AGGCCTACGT GCAGATCCTG 
AAGCAGCTGA CTGACAATCA CATCAGGTAC AGCGAAGAGA GGGGCTGGGA ACTGCTGTGG 
CTGTGCACGG GCCTCTTCCC GCCCAGCAAC ATCCTCCTGC CTCATGTTCA GCGGTTTCTG 
CAGTCCCGCA AGCACTGTCC TCTTGCCATT GACTGCCTGC AGAGGCTCCA GAAAGCCCTG 
AGAAATGGCT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAACATAAG 
ACTACCCAGA TCTTCCACAA GGTCTACTTC CCCGATGACA CGGACGAGGC TTTTGAGGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCAGCCGGCT GCTGCTCAAG 
TCTTCCGAGG GATTCAGCCT TTTTGTCAAA ATCGCAGATA AGGTCATCAG CGTCCCAGAG 
AATGATTTCT TCTTTGACTT TGTCCGACAC CTGACAGACT GGATAAAGAA AGCACGGCCC 
ATCAAGGACG GAATCGTGCC CTCACTAACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACAG TGCCGGGCAA GGACCCCATG GCTGACTCCA TCTTCCACTA TTACCAGGAA 
CTGCCCAAAT ATCTCCGAGG CTACCACAAG TGCACCCGGG AGGAGGTGCT GCAGCTGGGC 
GCACTCATCT ACAGGGTCAA GTTTGAGGAG GACAAATCCT ACTTCCCTAG CATCCCCAAG 
TTGCTGAGGG AGCTGGTACC CCAGGACCTA ATCCGGCAGG TCTCACCTGA TGACTGGAAA 
CGGTCTATTG TCGCCTACTT CAACAAACAT GCGGGGAAGT CCAAGGAGGA AGCCAAGCTG 
GCCTTCCTCA AACTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT TGAGGTGAAG 
CAAACTACAG AACCAAACTT CCCAGAGATT CTCTTAATTG CCATCAACAA GTACGGGGTC 
AGCCTCATCG ATCCCAGAAC CAAGGACATC CTGACTACTC ACCCCTTCAC CAAGATCTCC 
AACTGGAGTA GTGGCAACAC CTACTTCCAC ATCACCATTG GGAACTTGGT CCGTGGGAGC 
AAACTGCTCT GTGAGACATC GCTGGGATAC AAAATGGATG ATCTTCTGAC TTCCTACATC 
AGCCAGATGC TCACAGCCAT GAGCAAGCAG AGGAACTCCA GGAGTGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( NM_001256083.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243012.1 Mus musculus myosin VIIA (Myo7a), transcript variant 4, mRNA.

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CloneID	OMu20201
Clone ID Related Accession (Same CDS sequence)	XM_011241691.2 , NM_001256083.1 , XM_011241691.1
Accession Version	NM_001256083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115022.11, AY821853.1 and AC157792.4. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY821853.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGGAA CAAAGATGAT GGCCCCCAGC 
TTTACCCTGG CCACCATCAA AGGAGATGAG TACACCTTCA CATCCAGCAA TGCTGAGGAC 
ATCCGTGACC TGGTGGTCAC CTTTCTGGAG GGGCTACGGA AGAGGTCTAA GTATGTGGTG 
GCACTGCAGG ACAATCCTAA CCCTGCTGGT GAGGAGTCAG GCTTCCTCAG CTTCGCCAAG 
GGAGACCTCA TCATCCTTGA CCATGATACT GGTGAGCAGG TCATGAACTC AGGCTGGGCC 
AACGGCATCA ACGAGAGGAC CAAGCAGCGC GGCGACTTCC CCACTGACTG TGTATACGTC 
ATGCCCACTG TCACCTTGCC ACCAAGGGAG ATTGTGGCCC TGGTCACTAT GACCCCAGAC 
CAGAGGCAGG ATGTCGTCCG GCTCCTGCAG CTTCGCACAG CAGAGCCAGA GGTGCGCGCC 
AAGCCCTACA CGCTAGAGGA GTTCTCCTAC GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGC GGTAAGGACA GGCTGTGGAG CCACACACGA 
GAGCCCCTCA AGCAGGCCCT GCTCAAGAAG ATCCTGGGCA GTGAAGAACT CTCCCAGGAA 
GCCTGCATGG CCTTTGTAGC TGTGCTCAAG TACATGGGCG ACTACCCATC CAAGAGGATG 
CGATCCGTCA ATGAGCTCAC TGACCAGATC TTTGAGTGGG CACTCAAGGC TGAGCCCCTC 
AAGGATGAGG CCTACGTGCA GATCCTGAAG CAGCTGACTG ACAATCACAT CAGGTACAGC 
GAAGAGAGGG GCTGGGAACT GCTGTGGCTG TGCACGGGCC TCTTCCCGCC CAGCAACATC 
CTCCTGCCTC ATGTTCAGCG GTTTCTGCAG TCCCGCAAGC ACTGTCCTCT TGCCATTGAC 
TGCCTGCAGA GGCTCCAGAA AGCCCTGAGA AATGGCTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA ACATAAGACT ACCCAGATCT TCCACAAGGT CTACTTCCCC 
GATGACACGG ACGAGGCTTT TGAGGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA GCCGGCTGCT GCTCAAGTCT TCCGAGGGAT TCAGCCTTTT TGTCAAAATC 
GCAGATAAGG TCATCAGCGT CCCAGAGAAT GATTTCTTCT TTGACTTTGT CCGACACCTG 
ACAGACTGGA TAAAGAAAGC ACGGCCCATC AAGGACGGAA TCGTGCCCTC ACTAACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACAGTGC CGGGCAAGGA CCCCATGGCT 
GACTCCATCT TCCACTATTA CCAGGAACTG CCCAAATATC TCCGAGGCTA CCACAAGTGC 
ACCCGGGAGG AGGTGCTGCA GCTGGGCGCA CTCATCTACA GGGTCAAGTT TGAGGAGGAC 
AAATCCTACT TCCCTAGCAT CCCCAAGTTG CTGAGGGAGC TGGTACCCCA GGACCTAATC 
CGGCAGGTCT CACCTGATGA CTGGAAACGG TCTATTGTCG CCTACTTCAA CAAACATGCG 
GGGAAGTCCA AGGAGGAAGC CAAGCTGGCC TTCCTCAAAC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTTGA GGTGAAGCAA ACTACAGAAC CAAACTTCCC AGAGATTCTC 
TTAATTGCCA TCAACAAGTA CGGGGTCAGC CTCATCGATC CCAGAACCAA GGACATCCTG 
ACTACTCACC CCTTCACCAA GATCTCCAAC TGGAGTAGTG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTCCG TGGGAGCAAA CTGCTCTGTG AGACATCGCT GGGATACAAA 
ATGGATGATC TTCTGACTTC CTACATCAGC CAGATGCTCA CAGCCATGAG CAAGCAGAGG 
AACTCCAGGA GTGGAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243012.1
CDS	123..6623
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	NM_001256083.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256083.1

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGGAA CAAAGATGAT GGCCCCCAGC 
TTTACCCTGG CCACCATCAA AGGAGATGAG TACACCTTCA CATCCAGCAA TGCTGAGGAC 
ATCCGTGACC TGGTGGTCAC CTTTCTGGAG GGGCTACGGA AGAGGTCTAA GTATGTGGTG 
GCACTGCAGG ACAATCCTAA CCCTGCTGGT GAGGAGTCAG GCTTCCTCAG CTTCGCCAAG 
GGAGACCTCA TCATCCTTGA CCATGATACT GGTGAGCAGG TCATGAACTC AGGCTGGGCC 
AACGGCATCA ACGAGAGGAC CAAGCAGCGC GGCGACTTCC CCACTGACTG TGTATACGTC 
ATGCCCACTG TCACCTTGCC ACCAAGGGAG ATTGTGGCCC TGGTCACTAT GACCCCAGAC 
CAGAGGCAGG ATGTCGTCCG GCTCCTGCAG CTTCGCACAG CAGAGCCAGA GGTGCGCGCC 
AAGCCCTACA CGCTAGAGGA GTTCTCCTAC GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGC GGTAAGGACA GGCTGTGGAG CCACACACGA 
GAGCCCCTCA AGCAGGCCCT GCTCAAGAAG ATCCTGGGCA GTGAAGAACT CTCCCAGGAA 
GCCTGCATGG CCTTTGTAGC TGTGCTCAAG TACATGGGCG ACTACCCATC CAAGAGGATG 
CGATCCGTCA ATGAGCTCAC TGACCAGATC TTTGAGTGGG CACTCAAGGC TGAGCCCCTC 
AAGGATGAGG CCTACGTGCA GATCCTGAAG CAGCTGACTG ACAATCACAT CAGGTACAGC 
GAAGAGAGGG GCTGGGAACT GCTGTGGCTG TGCACGGGCC TCTTCCCGCC CAGCAACATC 
CTCCTGCCTC ATGTTCAGCG GTTTCTGCAG TCCCGCAAGC ACTGTCCTCT TGCCATTGAC 
TGCCTGCAGA GGCTCCAGAA AGCCCTGAGA AATGGCTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA ACATAAGACT ACCCAGATCT TCCACAAGGT CTACTTCCCC 
GATGACACGG ACGAGGCTTT TGAGGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA GCCGGCTGCT GCTCAAGTCT TCCGAGGGAT TCAGCCTTTT TGTCAAAATC 
GCAGATAAGG TCATCAGCGT CCCAGAGAAT GATTTCTTCT TTGACTTTGT CCGACACCTG 
ACAGACTGGA TAAAGAAAGC ACGGCCCATC AAGGACGGAA TCGTGCCCTC ACTAACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACAGTGC CGGGCAAGGA CCCCATGGCT 
GACTCCATCT TCCACTATTA CCAGGAACTG CCCAAATATC TCCGAGGCTA CCACAAGTGC 
ACCCGGGAGG AGGTGCTGCA GCTGGGCGCA CTCATCTACA GGGTCAAGTT TGAGGAGGAC 
AAATCCTACT TCCCTAGCAT CCCCAAGTTG CTGAGGGAGC TGGTACCCCA GGACCTAATC 
CGGCAGGTCT CACCTGATGA CTGGAAACGG TCTATTGTCG CCTACTTCAA CAAACATGCG 
GGGAAGTCCA AGGAGGAAGC CAAGCTGGCC TTCCTCAAAC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTTGA GGTGAAGCAA ACTACAGAAC CAAACTTCCC AGAGATTCTC 
TTAATTGCCA TCAACAAGTA CGGGGTCAGC CTCATCGATC CCAGAACCAA GGACATCCTG 
ACTACTCACC CCTTCACCAA GATCTCCAAC TGGAGTAGTG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTCCG TGGGAGCAAA CTGCTCTGTG AGACATCGCT GGGATACAAA 
ATGGATGATC TTCTGACTTC CTACATCAGC CAGATGCTCA CAGCCATGAG CAAGCAGAGG 
AACTCCAGGA GTGGAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241690.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239992.1 Mus musculus myosin VIIA (Myo7a), transcript variant X3, mRNA.

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CloneID	OMu20144
Clone ID Related Accession (Same CDS sequence)	XM_011241690.1 , XM_011241690.2 , NM_001256082.1
Accession Version	XM_011241690.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGGAACA 
AAGATGATGG CCCCCAGCTT TACCCTGGCC ACCATCAAAG GAGATGAGTA CACCTTCACA 
TCCAGCAATG CTGAGGACAT CCGTGACCTG GTGGTCACCT TTCTGGAGGG GCTACGGAAG 
AGGTCTAAGT ATGTGGTGGC ACTGCAGGAC AATCCTAACC CTGCTGGTGA GGAGTCAGGC 
TTCCTCAGCT TCGCCAAGGG AGACCTCATC ATCCTTGACC ATGATACTGG TGAGCAGGTC 
ATGAACTCAG GCTGGGCCAA CGGCATCAAC GAGAGGACCA AGCAGCGCGG CGACTTCCCC 
ACTGACTGTG TATACGTCAT GCCCACTGTC ACCTTGCCAC CAAGGGAGAT TGTGGCCCTG 
GTCACTATGA CCCCAGACCA GAGGCAGGAT GTCGTCCGGC TCCTGCAGCT TCGCACAGCA 
GAGCCAGAGG TGCGCGCCAA GCCCTACACG CTAGAGGAGT TCTCCTACGA CTACTTCAGG 
CCCCCACCCA AGCACACGCT GAGCCGTGTC ATGGTGTCCA AGGCCCGCGG TAAGGACAGG 
CTGTGGAGCC ACACACGAGA GCCCCTCAAG CAGGCCCTGC TCAAGAAGAT CCTGGGCAGT 
GAAGAACTCT CCCAGGAAGC CTGCATGGCC TTTGTAGCTG TGCTCAAGTA CATGGGCGAC 
TACCCATCCA AGAGGATGCG ATCCGTCAAT GAGCTCACTG ACCAGATCTT TGAGTGGGCA 
CTCAAGGCTG AGCCCCTCAA GGATGAGGCC TACGTGCAGA TCCTGAAGCA GCTGACTGAC 
AATCACATCA GGTACAGCGA AGAGAGGGGC TGGGAACTGC TGTGGCTGTG CACGGGCCTC 
TTCCCGCCCA GCAACATCCT CCTGCCTCAT GTTCAGCGGT TTCTGCAGTC CCGCAAGCAC 
TGTCCTCTTG CCATTGACTG CCTGCAGAGG CTCCAGAAAG CCCTGAGAAA TGGCTCCCGG 
AAGTACCCTC CGCACCTGGT GGAGGTGGAG GCCATCCAAC ATAAGACTAC CCAGATCTTC 
CACAAGGTCT ACTTCCCCGA TGACACGGAC GAGGCTTTTG AGGTGGAGTC CAGCACCAAG 
GCCAAGGACT TCTGCCAGAA CATCGCCAGC CGGCTGCTGC TCAAGTCTTC CGAGGGATTC 
AGCCTTTTTG TCAAAATCGC AGATAAGGTC ATCAGCGTCC CAGAGAATGA TTTCTTCTTT 
GACTTTGTCC GACACCTGAC AGACTGGATA AAGAAAGCAC GGCCCATCAA GGACGGAATC 
GTGCCCTCAC TAACCTACCA GGTGTTCTTC ATGAAGAAGC TGTGGACCAC CACAGTGCCG 
GGCAAGGACC CCATGGCTGA CTCCATCTTC CACTATTACC AGGAACTGCC CAAATATCTC 
CGAGGCTACC ACAAGTGCAC CCGGGAGGAG GTGCTGCAGC TGGGCGCACT CATCTACAGG 
GTCAAGTTTG AGGAGGACAA ATCCTACTTC CCTAGCATCC CCAAGTTGCT GAGGGAGCTG 
GTACCCCAGG ACCTAATCCG GCAGGTCTCA CCTGATGACT GGAAACGGTC TATTGTCGCC 
TACTTCAACA AACATGCGGG GAAGTCCAAG GAGGAAGCCA AGCTGGCCTT CCTCAAACTC 
ATCTTCAAGT GGCCCACCTT TGGCTCAGCC TTCTTTGAGG TGAAGCAAAC TACAGAACCA 
AACTTCCCAG AGATTCTCTT AATTGCCATC AACAAGTACG GGGTCAGCCT CATCGATCCC 
AGAACCAAGG ACATCCTGAC TACTCACCCC TTCACCAAGA TCTCCAACTG GAGTAGTGGC 
AACACCTACT TCCACATCAC CATTGGGAAC TTGGTCCGTG GGAGCAAACT GCTCTGTGAG 
ACATCGCTGG GATACAAAAT GGATGATCTT CTGACTTCCT ACATCAGCCA GATGCTCACA 
GCCATGAGCA AGCAGAGGAA CTCCAGGAGT GGAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239992.1
CDS	35..6553
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241690.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241690.1

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGGAACA 
AAGATGATGG CCCCCAGCTT TACCCTGGCC ACCATCAAAG GAGATGAGTA CACCTTCACA 
TCCAGCAATG CTGAGGACAT CCGTGACCTG GTGGTCACCT TTCTGGAGGG GCTACGGAAG 
AGGTCTAAGT ATGTGGTGGC ACTGCAGGAC AATCCTAACC CTGCTGGTGA GGAGTCAGGC 
TTCCTCAGCT TCGCCAAGGG AGACCTCATC ATCCTTGACC ATGATACTGG TGAGCAGGTC 
ATGAACTCAG GCTGGGCCAA CGGCATCAAC GAGAGGACCA AGCAGCGCGG CGACTTCCCC 
ACTGACTGTG TATACGTCAT GCCCACTGTC ACCTTGCCAC CAAGGGAGAT TGTGGCCCTG 
GTCACTATGA CCCCAGACCA GAGGCAGGAT GTCGTCCGGC TCCTGCAGCT TCGCACAGCA 
GAGCCAGAGG TGCGCGCCAA GCCCTACACG CTAGAGGAGT TCTCCTACGA CTACTTCAGG 
CCCCCACCCA AGCACACGCT GAGCCGTGTC ATGGTGTCCA AGGCCCGCGG TAAGGACAGG 
CTGTGGAGCC ACACACGAGA GCCCCTCAAG CAGGCCCTGC TCAAGAAGAT CCTGGGCAGT 
GAAGAACTCT CCCAGGAAGC CTGCATGGCC TTTGTAGCTG TGCTCAAGTA CATGGGCGAC 
TACCCATCCA AGAGGATGCG ATCCGTCAAT GAGCTCACTG ACCAGATCTT TGAGTGGGCA 
CTCAAGGCTG AGCCCCTCAA GGATGAGGCC TACGTGCAGA TCCTGAAGCA GCTGACTGAC 
AATCACATCA GGTACAGCGA AGAGAGGGGC TGGGAACTGC TGTGGCTGTG CACGGGCCTC 
TTCCCGCCCA GCAACATCCT CCTGCCTCAT GTTCAGCGGT TTCTGCAGTC CCGCAAGCAC 
TGTCCTCTTG CCATTGACTG CCTGCAGAGG CTCCAGAAAG CCCTGAGAAA TGGCTCCCGG 
AAGTACCCTC CGCACCTGGT GGAGGTGGAG GCCATCCAAC ATAAGACTAC CCAGATCTTC 
CACAAGGTCT ACTTCCCCGA TGACACGGAC GAGGCTTTTG AGGTGGAGTC CAGCACCAAG 
GCCAAGGACT TCTGCCAGAA CATCGCCAGC CGGCTGCTGC TCAAGTCTTC CGAGGGATTC 
AGCCTTTTTG TCAAAATCGC AGATAAGGTC ATCAGCGTCC CAGAGAATGA TTTCTTCTTT 
GACTTTGTCC GACACCTGAC AGACTGGATA AAGAAAGCAC GGCCCATCAA GGACGGAATC 
GTGCCCTCAC TAACCTACCA GGTGTTCTTC ATGAAGAAGC TGTGGACCAC CACAGTGCCG 
GGCAAGGACC CCATGGCTGA CTCCATCTTC CACTATTACC AGGAACTGCC CAAATATCTC 
CGAGGCTACC ACAAGTGCAC CCGGGAGGAG GTGCTGCAGC TGGGCGCACT CATCTACAGG 
GTCAAGTTTG AGGAGGACAA ATCCTACTTC CCTAGCATCC CCAAGTTGCT GAGGGAGCTG 
GTACCCCAGG ACCTAATCCG GCAGGTCTCA CCTGATGACT GGAAACGGTC TATTGTCGCC 
TACTTCAACA AACATGCGGG GAAGTCCAAG GAGGAAGCCA AGCTGGCCTT CCTCAAACTC 
ATCTTCAAGT GGCCCACCTT TGGCTCAGCC TTCTTTGAGG TGAAGCAAAC TACAGAACCA 
AACTTCCCAG AGATTCTCTT AATTGCCATC AACAAGTACG GGGTCAGCCT CATCGATCCC 
AGAACCAAGG ACATCCTGAC TACTCACCCC TTCACCAAGA TCTCCAACTG GAGTAGTGGC 
AACACCTACT TCCACATCAC CATTGGGAAC TTGGTCCGTG GGAGCAAACT GCTCTGTGAG 
ACATCGCTGG GATACAAAAT GGATGATCTT CTGACTTCCT ACATCAGCCA GATGCTCACA 
GCCATGAGCA AGCAGAGGAA CTCCAGGAGT GGAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241691.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239993.1 Mus musculus myosin VIIA (Myo7a), transcript variant X4, mRNA.

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CloneID	OMu20201
Clone ID Related Accession (Same CDS sequence)	XM_011241691.2 , NM_001256083.1 , XM_011241691.1
Accession Version	XM_011241691.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGGAA CAAAGATGAT GGCCCCCAGC 
TTTACCCTGG CCACCATCAA AGGAGATGAG TACACCTTCA CATCCAGCAA TGCTGAGGAC 
ATCCGTGACC TGGTGGTCAC CTTTCTGGAG GGGCTACGGA AGAGGTCTAA GTATGTGGTG 
GCACTGCAGG ACAATCCTAA CCCTGCTGGT GAGGAGTCAG GCTTCCTCAG CTTCGCCAAG 
GGAGACCTCA TCATCCTTGA CCATGATACT GGTGAGCAGG TCATGAACTC AGGCTGGGCC 
AACGGCATCA ACGAGAGGAC CAAGCAGCGC GGCGACTTCC CCACTGACTG TGTATACGTC 
ATGCCCACTG TCACCTTGCC ACCAAGGGAG ATTGTGGCCC TGGTCACTAT GACCCCAGAC 
CAGAGGCAGG ATGTCGTCCG GCTCCTGCAG CTTCGCACAG CAGAGCCAGA GGTGCGCGCC 
AAGCCCTACA CGCTAGAGGA GTTCTCCTAC GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGC GGTAAGGACA GGCTGTGGAG CCACACACGA 
GAGCCCCTCA AGCAGGCCCT GCTCAAGAAG ATCCTGGGCA GTGAAGAACT CTCCCAGGAA 
GCCTGCATGG CCTTTGTAGC TGTGCTCAAG TACATGGGCG ACTACCCATC CAAGAGGATG 
CGATCCGTCA ATGAGCTCAC TGACCAGATC TTTGAGTGGG CACTCAAGGC TGAGCCCCTC 
AAGGATGAGG CCTACGTGCA GATCCTGAAG CAGCTGACTG ACAATCACAT CAGGTACAGC 
GAAGAGAGGG GCTGGGAACT GCTGTGGCTG TGCACGGGCC TCTTCCCGCC CAGCAACATC 
CTCCTGCCTC ATGTTCAGCG GTTTCTGCAG TCCCGCAAGC ACTGTCCTCT TGCCATTGAC 
TGCCTGCAGA GGCTCCAGAA AGCCCTGAGA AATGGCTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA ACATAAGACT ACCCAGATCT TCCACAAGGT CTACTTCCCC 
GATGACACGG ACGAGGCTTT TGAGGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA GCCGGCTGCT GCTCAAGTCT TCCGAGGGAT TCAGCCTTTT TGTCAAAATC 
GCAGATAAGG TCATCAGCGT CCCAGAGAAT GATTTCTTCT TTGACTTTGT CCGACACCTG 
ACAGACTGGA TAAAGAAAGC ACGGCCCATC AAGGACGGAA TCGTGCCCTC ACTAACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACAGTGC CGGGCAAGGA CCCCATGGCT 
GACTCCATCT TCCACTATTA CCAGGAACTG CCCAAATATC TCCGAGGCTA CCACAAGTGC 
ACCCGGGAGG AGGTGCTGCA GCTGGGCGCA CTCATCTACA GGGTCAAGTT TGAGGAGGAC 
AAATCCTACT TCCCTAGCAT CCCCAAGTTG CTGAGGGAGC TGGTACCCCA GGACCTAATC 
CGGCAGGTCT CACCTGATGA CTGGAAACGG TCTATTGTCG CCTACTTCAA CAAACATGCG 
GGGAAGTCCA AGGAGGAAGC CAAGCTGGCC TTCCTCAAAC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTTGA GGTGAAGCAA ACTACAGAAC CAAACTTCCC AGAGATTCTC 
TTAATTGCCA TCAACAAGTA CGGGGTCAGC CTCATCGATC CCAGAACCAA GGACATCCTG 
ACTACTCACC CCTTCACCAA GATCTCCAAC TGGAGTAGTG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTCCG TGGGAGCAAA CTGCTCTGTG AGACATCGCT GGGATACAAA 
ATGGATGATC TTCTGACTTC CTACATCAGC CAGATGCTCA CAGCCATGAG CAAGCAGAGG 
AACTCCAGGA GTGGAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239993.1
CDS	35..6535
Translation	MDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241691.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241691.1

ORF Insert Sequence:

ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACGTACAC AGGTTCCATC CTGGTGGCCG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TACACCAACA AGAAGATAGG GGAGATGCCC 
CCCCACATCT TCGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAACCGGGAC 
CAGTGCTGTA TTATCAGCGG GGAGTCGGGA GCTGGCAAGA CAGAGAGCAC AAAGTTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGACAG CACTCATGGA TCGAGCAGCA GGTGCTGGAG 
GCCACCCCGA TCCTGGAAGC ATTTGGGAAC GCCAAGACCA TCCGCAACGA CAACTCTAGC 
CGCTTTGGCA AGTACATTGA CATCCACTTT AACAAGCGTG GTGCCATCGA GGGCGCCAAA 
ATAGAGCAAT ACCTGCTGGA GAAGTCACGT GTCTGCCGCC AGGCCCCTGA CGAGAGGAAC 
TATCACGTGT TCTACTGTAT GCTGGAGGGC ATGAATGAGG AGGAGAAGAA GAAACTGGGC 
CTAGGCCAGG CCGCTGACTA CAACTACTTG GCCATGGGTA ACTGCATCAC CTGTGAGGGC 
CGCGTGGACA GTCAGGAGTA TGCCAACATC CGCTCTGCCA TGAAGGTTCT CATGTTCACA 
GACACGGAGA ACTGGGAGAT CTCGAAGCTT CTGGCTGCCA TCCTACACAT GGGCAATCTG 
CAGTATGAGG CCCGGACATT TGAGAACTTG GATGCGTGTG AAGTCCTCTT CTCCCCATCG 
CTGGCCACGG CAGCTTCTCT GCTCGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTCACC 
AGCCGCACCC TCATCACCCG TGGGGAGACG GTGTCCACCC CTCTCAGCAG GGAACAGGCG 
CTGGATGTGC GAGATGCCTT TGTCAAGGGC ATCTATGGGC GGCTCTTTGT GTGGATTGTG 
GAGAAGATCA ACGCAGCAAT CTACAAGCCA CCCCCCCTGG AAGTGAAGAA CTCTCGCCGG 
TCCATCGGTC TCCTGGACAT CTTTGGATTT GAGAACTTCA CTGTGAACAG CTTCGAGCAG 
CTCTGCATTA ACTTTGCCAA TGAGCACCTG CAGCAATTCT TCGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAGTACGA CCTGGAGAGC ATCGACTGGT TGCACATTGA GTTCACTGAC 
AACCAGGAAG CACTGGACAT GATTGCCAAC CGGCCTATGA ACGTCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACGGAT GCCACCATGC TGCATAAGCT GAACTCACAG 
CACAAGCTCA ATGCCAACTA CGTGCCACCC AAGAACAGCC ACGAGACCCA GTTTGGAATC 
AACCACTTTG CGGGTGTTGT CTATTATGAG AGTCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATCAT CCAGCTGGTC CACTCTTCCC GGAACAAGTT CATAAAGCAG 
ATTTTCCAAG CTGACGTTGC CATGGGTGCC GAGACCAGGA AGCGCTCGCC TACACTCAGC 
AGCCAGTTCA AGCGGTCTCT GGAGCTGCTG ATGCGCACAC TGGGCGCCTG CCAGCCCTTC 
TTTGTGCGTT GTATCAAACC CAATGAGTTC AAGAAGCCCA TGCTCTTCGA CCGGCACTTG 
TGTGTACGCC AGCTGCGATA TTCGGGCATG ATGGAGACAA TCCGCATCCG CCACGCAGGC 
TACCCCATTC GCTACAGCTT TGTGGAGTTT GTGGAGCGCT ACCGGGTACT GCTGCCTGGT 
GTGAAGCCAG CATACAAGCA GGGTGACCTC CGAGGGACAT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CGCACGATGA CTGGCAGATT GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGT TGCTGGAGGT GGAGCGGGAC AAGGCCATCA CAGACAGAGT CATTCTCCTC 
CAGAAGGTTA TCCGGGGCTT CAAAGACAGG TCCAACTTCC TGAGACTGAA GAGTGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGCCAC CACTGTAGGA AAAACTATGA GCTGATTCGT 
CTTGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACAA GCAGTACCGC 
CTGGCCAGAC AGCGCATAAT AGAGTTCCAG GCCCGCTGCC GGGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCCGTGATC ACCGTGCAGG CCTATGCCCG AGGCATGATT 
GCCCGCCGGC TACACCGCCG CCTCCGGGTT GAGTACCAGC GGCGCCTCGA GGCAGAGAGG 
ATGCGTCTGG CAGAGGAGGA GAAACTCCGA AAGGAGATGA GTGCCAAGAA GGCCAAAGAG 
GAGGCTGAGC GCAAGCATCA GGAGCGCCTG GCTCAGCTAG CCCGCGAGGA TGCGGAGCGG 
GAACTGAAGG AGAAGGAGGA GGCTCGGAGG AAGAAGGAAC TGCTGGAGCA GATGGAGAAG 
GCCCGCCACG AACCCATCAA CCACTCAGAT ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGCA GCCTGCCAGG CCAGGAAGGC CAGGCGCCTA GTGGCTTTGA GGACCTAGAG 
CGCGGACGGA GGGAGATGGT GGAAGAGGAT GTTGACGCTG CCCTGCCCCT GCCTGATGAA 
GACGAGGAGG ACCTTTCTGA GTACAAATTC GCCAAGTTTG CTGCCACCTA CTTCCAGGGC 
ACAACCACAC ACTCCTACAC CCGGAGGCCT CTCAAGCAGC CGCTGCTCTA CCACGACGAT 
GAGGGTGACC AGCTGGCGGC GCTGGCTGTC TGGATCACCA TCCTCCGGTT CATGGGGGAC 
CTCCCAGAGC CCAAGTACCA CACAGCCATG AGCGACGGCA GTGAGAAGAT CCCAGTGATG 
ACTAAGATCT ACGAGACCCT AGGCAAGAAG ACATATAAGA GGGAGCTGCA GGCCTTGCAG 
GGCGAGGGCG AGACCCAGCT CCCTGAGGGG CAGAAGAAGA CCAGTGTGAG ACACAAGTTG 
GTACACTTGA CACTGAAGAA AAAGTCCAAA CTCACAGAAG AGGTGACCAA GAGGCTGAAC 
GATGGGGAAT CCACGGTACA GGGCAACAGC ATGCTGGAGG ATCGGCCCAC CTCAAATCTA 
GAGAAGCTGC ACTTCATCAT CGGCAACGGC ATCCTGCGGC CTGCGCTGCG GGACGAGATT 
TACTGCCAGA TCAGTAAGCA GCTCACACAC AACCCATCCA AGAGCAGCTA TGCCAGGGGC 
TGGATCCTCG TGTCGCTCTG TGTGGGCTGC TTCGCCCCCT CTGAGAAGTT CGTTAAGTAC 
CTGCGGAACT TCATCCACGG AGGCCCACCT GGCTATGCTC CTTACTGTGA GGAGCGCCTG 
AGGAGGACCT TTGTCAACGG AACTCGGACA CAGCCACCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCCAT CATGTTGCCC GTGACCTTCA TGGATGGGAC CACCAAGACC 
CTGCTAACAG ATTCAGCAAC TACAGCCAGG GAGCTGTGCA ATGCTCTGGC TGACAAGATC 
TCACTCAAGG ACCGCTTTGG CTTCTCCCTC TACATCGCTC TGTTCGATAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA TGTCATGGAT GCCATCTCTC AGTGTGAGCA GTACGCCAAG 
GAGCAGGGTG CTCAGGAGCG CAACGCCCCA TGGAGGCTCT TCTTTAGAAA GGAGGTCTTC 
ACACCCTGGC ACAACCCCTC GGAGGACAAC GTGGCCACGA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCTTCTCAGC AGTACTTTGT GGACTATGGT TCTGAGATGA TTCTGGAGCG CCTGCTGAGC 
CTCGTGCCCA CTTACATCCC TGACCGTGAG ATCACACCGC TGAAGAATCT TGAGAAGTGG 
GCACAGCTGG CCATTGCTGC CCACAAGAAG GGAATTTATG CCCAGAGGAG AACTGACTCC 
CAGAAGGTCA AAGAGGATGT GGTCAATTAT GCCCGTTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTACG AAGCTTACAA ATTCTCAGGC CCTCCCCTCC CCAAGAGCGA CGTCATCGTG 
GCTGTCAACT GGACGGGTGT GTACTTCGTG GACGAGCAGG AGCAGGTGCT TCTGGAGCTG 
TCCTTCCCGG AGATCATGGC TGTGTCCAGC AGTAGGGGAA CAAAGATGAT GGCCCCCAGC 
TTTACCCTGG CCACCATCAA AGGAGATGAG TACACCTTCA CATCCAGCAA TGCTGAGGAC 
ATCCGTGACC TGGTGGTCAC CTTTCTGGAG GGGCTACGGA AGAGGTCTAA GTATGTGGTG 
GCACTGCAGG ACAATCCTAA CCCTGCTGGT GAGGAGTCAG GCTTCCTCAG CTTCGCCAAG 
GGAGACCTCA TCATCCTTGA CCATGATACT GGTGAGCAGG TCATGAACTC AGGCTGGGCC 
AACGGCATCA ACGAGAGGAC CAAGCAGCGC GGCGACTTCC CCACTGACTG TGTATACGTC 
ATGCCCACTG TCACCTTGCC ACCAAGGGAG ATTGTGGCCC TGGTCACTAT GACCCCAGAC 
CAGAGGCAGG ATGTCGTCCG GCTCCTGCAG CTTCGCACAG CAGAGCCAGA GGTGCGCGCC 
AAGCCCTACA CGCTAGAGGA GTTCTCCTAC GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGC GGTAAGGACA GGCTGTGGAG CCACACACGA 
GAGCCCCTCA AGCAGGCCCT GCTCAAGAAG ATCCTGGGCA GTGAAGAACT CTCCCAGGAA 
GCCTGCATGG CCTTTGTAGC TGTGCTCAAG TACATGGGCG ACTACCCATC CAAGAGGATG 
CGATCCGTCA ATGAGCTCAC TGACCAGATC TTTGAGTGGG CACTCAAGGC TGAGCCCCTC 
AAGGATGAGG CCTACGTGCA GATCCTGAAG CAGCTGACTG ACAATCACAT CAGGTACAGC 
GAAGAGAGGG GCTGGGAACT GCTGTGGCTG TGCACGGGCC TCTTCCCGCC CAGCAACATC 
CTCCTGCCTC ATGTTCAGCG GTTTCTGCAG TCCCGCAAGC ACTGTCCTCT TGCCATTGAC 
TGCCTGCAGA GGCTCCAGAA AGCCCTGAGA AATGGCTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA ACATAAGACT ACCCAGATCT TCCACAAGGT CTACTTCCCC 
GATGACACGG ACGAGGCTTT TGAGGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA GCCGGCTGCT GCTCAAGTCT TCCGAGGGAT TCAGCCTTTT TGTCAAAATC 
GCAGATAAGG TCATCAGCGT CCCAGAGAAT GATTTCTTCT TTGACTTTGT CCGACACCTG 
ACAGACTGGA TAAAGAAAGC ACGGCCCATC AAGGACGGAA TCGTGCCCTC ACTAACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACAGTGC CGGGCAAGGA CCCCATGGCT 
GACTCCATCT TCCACTATTA CCAGGAACTG CCCAAATATC TCCGAGGCTA CCACAAGTGC 
ACCCGGGAGG AGGTGCTGCA GCTGGGCGCA CTCATCTACA GGGTCAAGTT TGAGGAGGAC 
AAATCCTACT TCCCTAGCAT CCCCAAGTTG CTGAGGGAGC TGGTACCCCA GGACCTAATC 
CGGCAGGTCT CACCTGATGA CTGGAAACGG TCTATTGTCG CCTACTTCAA CAAACATGCG 
GGGAAGTCCA AGGAGGAAGC CAAGCTGGCC TTCCTCAAAC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTTGA GGTGAAGCAA ACTACAGAAC CAAACTTCCC AGAGATTCTC 
TTAATTGCCA TCAACAAGTA CGGGGTCAGC CTCATCGATC CCAGAACCAA GGACATCCTG 
ACTACTCACC CCTTCACCAA GATCTCCAAC TGGAGTAGTG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTCCG TGGGAGCAAA CTGCTCTGTG AGACATCGCT GGGATACAAA 
ATGGATGATC TTCTGACTTC CTACATCAGC CAGATGCTCA CAGCCATGAG CAAGCAGAGG 
AACTCCAGGA GTGGAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_006507427.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507490.1 Mus musculus myosin VIIA (Myo7a), transcript variant X5, mRNA.

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CloneID	OMu39357
Clone ID Related Accession (Same CDS sequence)	XM_006507427.4 , XM_006507427.3
Accession Version	XM_006507427.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507427.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACCAG CTGTGGAGGA 
CGGACGTACA CAGGTTCCAT CCTGGTGGCC GTGAACCCCT ACCAGCTGCT CTCCATCTAC 
TCGCCAGAGC ACATCCGCCA GTACACCAAC AAGAAGATAG GGGAGATGCC CCCCCACATC 
TTCGCCATTG CTGACAACTG CTACTTCAAC ATGAAACGCA ACAACCGGGA CCAGTGCTGT 
ATTATCAGCG GGGAGTCGGG AGCTGGCAAG ACAGAGAGCA CAAAGTTGAT CCTGCAGTTC 
CTGGCAGCCA TCAGTGGACA GCACTCATGG ATCGAGCAGC AGGTGCTGGA GGCCACCCCG 
ATCCTGGAAG CATTTGGGAA CGCCAAGACC ATCCGCAACG ACAACTCTAG CCGCTTTGGC 
AAGTACATTG ACATCCACTT TAACAAGCGT GGTGCCATCG AGGGCGCCAA AATAGAGCAA 
TACCTGCTGG AGAAGTCACG TGTCTGCCGC CAGGCCCCTG ACGAGAGGAA CTATCACGTG 
TTCTACTGTA TGCTGGAGGG CATGAATGAG GAGGAGAAGA AGAAACTGGG CCTAGGCCAG 
GCCGCTGACT ACAACTACTT GGCCATGGGT AACTGCATCA CCTGTGAGGG CCGCGTGGAC 
AGTCAGGAGT ATGCCAACAT CCGCTCTGCC ATGAAGGTTC TCATGTTCAC AGACACGGAG 
AACTGGGAGA TCTCGAAGCT TCTGGCTGCC ATCCTACACA TGGGCAATCT GCAGTATGAG 
GCCCGGACAT TTGAGAACTT GGATGCGTGT GAAGTCCTCT TCTCCCCATC GCTGGCCACG 
GCAGCTTCTC TGCTCGAGGT GAACCCCCCA GACCTGATGA GCTGCCTCAC CAGCCGCACC 
CTCATCACCC GTGGGGAGAC GGTGTCCACC CCTCTCAGCA GGGAACAGGC GCTGGATGTG 
CGAGATGCCT TTGTCAAGGG CATCTATGGG CGGCTCTTTG TGTGGATTGT GGAGAAGATC 
AACGCAGCAA TCTACAAGCC ACCCCCCCTG GAAGTGAAGA ACTCTCGCCG GTCCATCGGT 
CTCCTGGACA TCTTTGGATT TGAGAACTTC ACTGTGAACA GCTTCGAGCA GCTCTGCATT 
AACTTTGCCA ATGAGCACCT GCAGCAATTC TTCGTGCGGC ACGTGTTCAA GCTGGAGCAG 
GAGGAGTACG ACCTGGAGAG CATCGACTGG TTGCACATTG AGTTCACTGA CAACCAGGAA 
GCACTGGACA TGATTGCCAA CCGGCCTATG AACGTCATCT CCCTCATCGA TGAGGAGAGC 
AAGTTCCCCA AGGGCACGGA TGCCACCATG CTGCATAAGC TGAACTCACA GCACAAGCTC 
AATGCCAACT ACGTGCCACC CAAGAACAGC CACGAGACCC AGTTTGGAAT CAACCACTTT 
GCGGGTGTTG TCTATTATGA GAGTCAAGGC TTCCTGGAGA AGAACCGAGA CACCCTGCAT 
GGGGACATCA TCCAGCTGGT CCACTCTTCC CGGAACAAGT TCATAAAGCA GATTTTCCAA 
GCTGACGTTG CCATGGGTGC CGAGACCAGG AAGCGCTCGC CTACACTCAG CAGCCAGTTC 
AAGCGGTCTC TGGAGCTGCT GATGCGCACA CTGGGCGCCT GCCAGCCCTT CTTTGTGCGT 
TGTATCAAAC CCAATGAGTT CAAGAAGCCC ATGCTCTTCG ACCGGCACTT GTGTGTACGC 
CAGCTGCGAT ATTCGGGCAT GATGGAGACA ATCCGCATCC GCCACGCAGG CTACCCCATT 
CGCTACAGCT TTGTGGAGTT TGTGGAGCGC TACCGGGTAC TGCTGCCTGG TGTGAAGCCA 
GCATACAAGC AGGGTGACCT CCGAGGGACA TGCCAGCGCA TGGCTGAGGC TGTGCTGGGC 
ACGCACGATG ACTGGCAGAT TGGCAAAACC AAGATCTTTC TGAAGGACCA CCATGACATG 
TTGCTGGAGG TGGAGCGGGA CAAGGCCATC ACAGACAGAG TCATTCTCCT CCAGAAGGTT 
ATCCGGGGCT TCAAAGACAG GTCCAACTTC CTGAGACTGA AGAGTGCTGC CACACTGATC 
CAGAGGCACT GGCGGGGCCA CCACTGTAGG AAAAACTATG AGCTGATTCG TCTTGGCTTC 
CTGCGGCTGC AGGCCCTGCA CCGCTCCCGG AAGCTGCACA AGCAGTACCG CCTGGCCAGA 
CAGCGCATAA TAGAGTTCCA GGCCCGCTGC CGGGCCTATC TGGTGCGCAA GGCCTTCCGC 
CACCGCCTCT GGGCCGTGAT CACCGTGCAG GCCTATGCCC GAGGCATGAT TGCCCGCCGG 
CTACACCGCC GCCTCCGGGT TGAGTACCAG CGGCGCCTCG AGGCAGAGAG GATGCGTCTG 
GCAGAGGAGG AGAAACTCCG AAAGGAGATG AGTGCCAAGA AGGCCAAAGA GGAGGCTGAG 
CGCAAGCATC AGGAGCGCCT GGCTCAGCTA GCCCGCGAGG ATGCGGAGCG GGAACTGAAG 
GAGAAGGAGG AGGCTCGGAG GAAGAAGGAA CTGCTGGAGC AGATGGAGAA GGCCCGCCAC 
GAACCCATCA ACCACTCAGA TATGGTGGAC AAGATGTTTG GCTTCCTGGG GACTTCAGGC 
AGCCTGCCAG GCCAGGAAGG CCAGGCGCCT AGTGGCTTTG AGGACCTAGA GCGCGGACGG 
AGGGAGATGG TGGAAGAGGA TGTTGACGCT GCCCTGCCCC TGCCTGATGA AGACGAGGAG 
GACCTTTCTG AGTACAAATT CGCCAAGTTT GCTGCCACCT ACTTCCAGGG CACAACCACA 
CACTCCTACA CCCGGAGGCC TCTCAAGCAG CCGCTGCTCT ACCACGACGA TGAGGGTGAC 
CAGCTGGCGG CGCTGGCTGT CTGGATCACC ATCCTCCGGT TCATGGGGGA CCTCCCAGAG 
CCCAAGTACC ACACAGCCAT GAGCGACGGC AGTGAGAAGA TCCCAGTGAT GACTAAGATC 
TACGAGACCC TAGGCAAGAA GACATATAAG AGGGAGCTGC AGGCCTTGCA GGGCGAGGGC 
GAGACCCAGC TCCCTGAGGG GCAGAAGAAG ACCAGTGTGA GACACAAGTT GGTACACTTG 
ACACTGAAGA AAAAGTCCAA ACTCACAGAA GAGGTGACCA AGAGGCTGAA CGATGGGGAA 
TCCACGGTAC AGGGCAACAG CATGCTGGAG GATCGGCCCA CCTCAAATCT AGAGAAGCTG 
CACTTCATCA TCGGCAACGG CATCCTGCGG CCTGCGCTGC GGGACGAGAT TTACTGCCAG 
ATCAGTAAGC AGCTCACACA CAACCCATCC AAGAGCAGCT ATGCCAGGGG CTGGATCCTC 
GTGTCGCTCT GTGTGGGCTG CTTCGCCCCC TCTGAGAAGT TCGTTAAGTA CCTGCGGAAC 
TTCATCCACG GAGGCCCACC TGGCTATGCT CCTTACTGTG AGGAGCGCCT GAGGAGGACC 
TTTGTCAACG GAACTCGGAC ACAGCCACCC AGCTGGCTGG AGCTGCAGGC CACCAAGTCC 
AAGAAGCCCA TCATGTTGCC CGTGACCTTC ATGGATGGGA CCACCAAGAC CCTGCTAACA 
GATTCAGCAA CTACAGCCAG GGAGCTGTGC AATGCTCTGG CTGACAAGAT CTCACTCAAG 
GACCGCTTTG GCTTCTCCCT CTACATCGCT CTGTTCGATA AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ATGTCATGGA TGCCATCTCT CAGTGTGAGC AGTACGCCAA GGAGCAGGGT 
GCTCAGGAGC GCAACGCCCC ATGGAGGCTC TTCTTTAGAA AGGAGGTCTT CACACCCTGG 
CACAACCCCT CGGAGGACAA CGTGGCCACG AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCTTCTCAG 
CAGTACTTTG TGGACTATGG TTCTGAGATG ATTCTGGAGC GCCTGCTGAG CCTCGTGCCC 
ACTTACATCC CTGACCGTGA GATCACACCG CTGAAGAATC TTGAGAAGTG GGCACAGCTG 
GCCATTGCTG CCCACAAGAA GGGAATTTAT GCCCAGAGGA GAACTGACTC CCAGAAGGTC 
AAAGAGGATG TGGTCAATTA TGCCCGTTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAC 
GAAGCTTACA AATTCTCAGG CCCTCCCCTC CCCAAGAGCG ACGTCATCGT GGCTGTCAAC 
TGGACGGGTG TGTACTTCGT GGACGAGCAG GAGCAGGTGC TTCTGGAGCT GTCCTTCCCG 
GAGATCATGG CTGTGTCCAG CAGTAGGGAG TGCCGCGTCT TGCTCTCACT GGGCTGCTCT 
GACTTGGGCT GTGCTACTTG TCAATCGGGC CGGGCAGGGC TGACCCCGGC TGGACCCTGT 
TCTCCGTGTT GGTCCTGTAG GGGAACAAAG ATGATGGCCC CCAGCTTTAC CCTGGCCACC 
ATCAAAGGAG ATGAGTACAC CTTCACATCC AGCAATGCTG AGGACATCCG TGACCTGGTG 
GTCACCTTTC TGGAGGGGCT ACGGAAGAGG TCTAAGTATG TGGTGGCACT GCAGGACAAT 
CCTAACCCTG CTGGTGAGGA GTCAGGCTTC CTCAGCTTCG CCAAGGGAGA CCTCATCATC 
CTTGACCATG ATACTGGTGA GCAGGTCATG AACTCAGGCT GGGCCAACGG CATCAACGAG 
AGGACCAAGC AGCGCGGCGA CTTCCCCACT GACTGTGTAT ACGTCATGCC CACTGTCACC 
TTGCCACCAA GGGAGATTGT GGCCCTGGTC ACTATGACCC CAGACCAGAG GCAGGATGTC 
GTCCGGCTCC TGCAGCTTCG CACAGCAGAG CCAGAGGTGC GCGCCAAGCC CTACACGCTA 
GAGGAGTTCT CCTACGACTA CTTCAGGCCC CCACCCAAGC ACACGCTGAG CCGTGTCATG 
GTGTCCAAGG CCCGCGGTAA GGACAGGCTG TGGAGCCACA CACGAGAGCC CCTCAAGCAG 
GCCCTGCTCA AGAAGATCCT GGGCAGTGAA GAACTCTCCC AGGAAGCCTG CATGGCCTTT 
GTAGCTGTGC TCAAGTACAT GGGCGACTAC CCATCCAAGA GGATGCGATC CGTCAATGAG 
CTCACTGACC AGATCTTTGA GTGGGCACTC AAGGCTGAGC CCCTCAAGGA TGAGGCCTAC 
GTGCAGATCC TGAAGCAGCT GACTGACAAT CACATCAGGT ACAGCGAAGA GAGGGGCTGG 
GAACTGCTGT GGCTGTGCAC GGGCCTCTTC CCGCCCAGCA ACATCCTCCT GCCTCATGTT 
CAGCGGTTTC TGCAGTCCCG CAAGCACTGT CCTCTTGCCA TTGACTGCCT GCAGAGGCTC 
CAGAAAGCCC TGAGAAATGG CTCCCGGAAG TACCCTCCGC ACCTGGTGGA GGTGGAGGCC 
ATCCAACATA AGACTACCCA GATCTTCCAC AAGGTCTACT TCCCCGATGA CACGGACGAG 
GCTTTTGAGG TGGAGTCCAG CACCAAGGCC AAGGACTTCT GCCAGAACAT CGCCAGCCGG 
CTGCTGCTCA AGTCTTCCGA GGGATTCAGC CTTTTTGTCA AAATCGCAGA TAAGGTCATC 
AGCGTCCCAG AGAATGATTT CTTCTTTGAC TTTGTCCGAC ACCTGACAGA CTGGATAAAG 
AAAGCACGGC CCATCAAGGA CGGAATCGTG CCCTCACTAA CCTACCAGGT GTTCTTCATG 
AAGAAGCTGT GGACCACCAC AGTGCCGGGC AAGGACCCCA TGGCTGACTC CATCTTCCAC 
TATTACCAGG AACTGCCCAA ATATCTCCGA GGCTACCACA AGTGCACCCG GGAGGAGGTG 
CTGCAGCTGG GCGCACTCAT CTACAGGGTC AAGTTTGAGG AGGACAAATC CTACTTCCCT 
AGCATCCCCA AGTTGCTGAG GGAGCTGGTA CCCCAGGACC TAATCCGGCA GGTCTCACCT 
GATGACTGGA AACGGTCTAT TGTCGCCTAC TTCAACAAAC ATGCGGGGAA GTCCAAGGAG 
GAAGCCAAGC TGGCCTTCCT CAAACTCATC TTCAAGTGGC CCACCTTTGG CTCAGCCTTC 
TTTGAGGTGA AGCAAACTAC AGAACCAAAC TTCCCAGAGA TTCTCTTAAT TGCCATCAAC 
AAGTACGGGG TCAGCCTCAT CGATCCCAGA ACCAAGGACA TCCTGACTAC TCACCCCTTC 
ACCAAGATCT CCAACTGGAG TAGTGGCAAC ACCTACTTCC ACATCACCAT TGGGAACTTG 
GTCCGTGGGA GCAAACTGCT CTGTGAGACA TCGCTGGGAT ACAAAATGGA TGATCTTCTG 
ACTTCCTACA TCAGCCAGAT GCTCACAGCC ATGAGCAAGC AGAGGAACTC CAGGAGTGGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507490.1
CDS	292..6957
Translation	MVILQKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_006507427.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507427.3

ORF Insert Sequence:

ATGGTTATTC TGCAGAAGGG GGACTATGTA TGGATGGACC TGAAGTCAGG CCAGGAGTTT 
GATGTGCCCA TCGGGGCCGT GGTGAAGCTC TGCGACTCGG GCCAGATCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATATCCCCT CAGAATGCCA CGCACATCAA GCCAATGCAC 
CCCACATCGG TGCACGGCGT GGAGGACATG ATCCGCCTGG GGGATCTCAA CGAGGCAGGC 
ATCCTTCGAA ACCTTCTCAT TCGCTACCGG GACCACCTCA TCTATACCAG CTGTGGAGGA 
CGGACGTACA CAGGTTCCAT CCTGGTGGCC GTGAACCCCT ACCAGCTGCT CTCCATCTAC 
TCGCCAGAGC ACATCCGCCA GTACACCAAC AAGAAGATAG GGGAGATGCC CCCCCACATC 
TTCGCCATTG CTGACAACTG CTACTTCAAC ATGAAACGCA ACAACCGGGA CCAGTGCTGT 
ATTATCAGCG GGGAGTCGGG AGCTGGCAAG ACAGAGAGCA CAAAGTTGAT CCTGCAGTTC 
CTGGCAGCCA TCAGTGGACA GCACTCATGG ATCGAGCAGC AGGTGCTGGA GGCCACCCCG 
ATCCTGGAAG CATTTGGGAA CGCCAAGACC ATCCGCAACG ACAACTCTAG CCGCTTTGGC 
AAGTACATTG ACATCCACTT TAACAAGCGT GGTGCCATCG AGGGCGCCAA AATAGAGCAA 
TACCTGCTGG AGAAGTCACG TGTCTGCCGC CAGGCCCCTG ACGAGAGGAA CTATCACGTG 
TTCTACTGTA TGCTGGAGGG CATGAATGAG GAGGAGAAGA AGAAACTGGG CCTAGGCCAG 
GCCGCTGACT ACAACTACTT GGCCATGGGT AACTGCATCA CCTGTGAGGG CCGCGTGGAC 
AGTCAGGAGT ATGCCAACAT CCGCTCTGCC ATGAAGGTTC TCATGTTCAC AGACACGGAG 
AACTGGGAGA TCTCGAAGCT TCTGGCTGCC ATCCTACACA TGGGCAATCT GCAGTATGAG 
GCCCGGACAT TTGAGAACTT GGATGCGTGT GAAGTCCTCT TCTCCCCATC GCTGGCCACG 
GCAGCTTCTC TGCTCGAGGT GAACCCCCCA GACCTGATGA GCTGCCTCAC CAGCCGCACC 
CTCATCACCC GTGGGGAGAC GGTGTCCACC CCTCTCAGCA GGGAACAGGC GCTGGATGTG 
CGAGATGCCT TTGTCAAGGG CATCTATGGG CGGCTCTTTG TGTGGATTGT GGAGAAGATC 
AACGCAGCAA TCTACAAGCC ACCCCCCCTG GAAGTGAAGA ACTCTCGCCG GTCCATCGGT 
CTCCTGGACA TCTTTGGATT TGAGAACTTC ACTGTGAACA GCTTCGAGCA GCTCTGCATT 
AACTTTGCCA ATGAGCACCT GCAGCAATTC TTCGTGCGGC ACGTGTTCAA GCTGGAGCAG 
GAGGAGTACG ACCTGGAGAG CATCGACTGG TTGCACATTG AGTTCACTGA CAACCAGGAA 
GCACTGGACA TGATTGCCAA CCGGCCTATG AACGTCATCT CCCTCATCGA TGAGGAGAGC 
AAGTTCCCCA AGGGCACGGA TGCCACCATG CTGCATAAGC TGAACTCACA GCACAAGCTC 
AATGCCAACT ACGTGCCACC CAAGAACAGC CACGAGACCC AGTTTGGAAT CAACCACTTT 
GCGGGTGTTG TCTATTATGA GAGTCAAGGC TTCCTGGAGA AGAACCGAGA CACCCTGCAT 
GGGGACATCA TCCAGCTGGT CCACTCTTCC CGGAACAAGT TCATAAAGCA GATTTTCCAA 
GCTGACGTTG CCATGGGTGC CGAGACCAGG AAGCGCTCGC CTACACTCAG CAGCCAGTTC 
AAGCGGTCTC TGGAGCTGCT GATGCGCACA CTGGGCGCCT GCCAGCCCTT CTTTGTGCGT 
TGTATCAAAC CCAATGAGTT CAAGAAGCCC ATGCTCTTCG ACCGGCACTT GTGTGTACGC 
CAGCTGCGAT ATTCGGGCAT GATGGAGACA ATCCGCATCC GCCACGCAGG CTACCCCATT 
CGCTACAGCT TTGTGGAGTT TGTGGAGCGC TACCGGGTAC TGCTGCCTGG TGTGAAGCCA 
GCATACAAGC AGGGTGACCT CCGAGGGACA TGCCAGCGCA TGGCTGAGGC TGTGCTGGGC 
ACGCACGATG ACTGGCAGAT TGGCAAAACC AAGATCTTTC TGAAGGACCA CCATGACATG 
TTGCTGGAGG TGGAGCGGGA CAAGGCCATC ACAGACAGAG TCATTCTCCT CCAGAAGGTT 
ATCCGGGGCT TCAAAGACAG GTCCAACTTC CTGAGACTGA AGAGTGCTGC CACACTGATC 
CAGAGGCACT GGCGGGGCCA CCACTGTAGG AAAAACTATG AGCTGATTCG TCTTGGCTTC 
CTGCGGCTGC AGGCCCTGCA CCGCTCCCGG AAGCTGCACA AGCAGTACCG CCTGGCCAGA 
CAGCGCATAA TAGAGTTCCA GGCCCGCTGC CGGGCCTATC TGGTGCGCAA GGCCTTCCGC 
CACCGCCTCT GGGCCGTGAT CACCGTGCAG GCCTATGCCC GAGGCATGAT TGCCCGCCGG 
CTACACCGCC GCCTCCGGGT TGAGTACCAG CGGCGCCTCG AGGCAGAGAG GATGCGTCTG 
GCAGAGGAGG AGAAACTCCG AAAGGAGATG AGTGCCAAGA AGGCCAAAGA GGAGGCTGAG 
CGCAAGCATC AGGAGCGCCT GGCTCAGCTA GCCCGCGAGG ATGCGGAGCG GGAACTGAAG 
GAGAAGGAGG AGGCTCGGAG GAAGAAGGAA CTGCTGGAGC AGATGGAGAA GGCCCGCCAC 
GAACCCATCA ACCACTCAGA TATGGTGGAC AAGATGTTTG GCTTCCTGGG GACTTCAGGC 
AGCCTGCCAG GCCAGGAAGG CCAGGCGCCT AGTGGCTTTG AGGACCTAGA GCGCGGACGG 
AGGGAGATGG TGGAAGAGGA TGTTGACGCT GCCCTGCCCC TGCCTGATGA AGACGAGGAG 
GACCTTTCTG AGTACAAATT CGCCAAGTTT GCTGCCACCT ACTTCCAGGG CACAACCACA 
CACTCCTACA CCCGGAGGCC TCTCAAGCAG CCGCTGCTCT ACCACGACGA TGAGGGTGAC 
CAGCTGGCGG CGCTGGCTGT CTGGATCACC ATCCTCCGGT TCATGGGGGA CCTCCCAGAG 
CCCAAGTACC ACACAGCCAT GAGCGACGGC AGTGAGAAGA TCCCAGTGAT GACTAAGATC 
TACGAGACCC TAGGCAAGAA GACATATAAG AGGGAGCTGC AGGCCTTGCA GGGCGAGGGC 
GAGACCCAGC TCCCTGAGGG GCAGAAGAAG ACCAGTGTGA GACACAAGTT GGTACACTTG 
ACACTGAAGA AAAAGTCCAA ACTCACAGAA GAGGTGACCA AGAGGCTGAA CGATGGGGAA 
TCCACGGTAC AGGGCAACAG CATGCTGGAG GATCGGCCCA CCTCAAATCT AGAGAAGCTG 
CACTTCATCA TCGGCAACGG CATCCTGCGG CCTGCGCTGC GGGACGAGAT TTACTGCCAG 
ATCAGTAAGC AGCTCACACA CAACCCATCC AAGAGCAGCT ATGCCAGGGG CTGGATCCTC 
GTGTCGCTCT GTGTGGGCTG CTTCGCCCCC TCTGAGAAGT TCGTTAAGTA CCTGCGGAAC 
TTCATCCACG GAGGCCCACC TGGCTATGCT CCTTACTGTG AGGAGCGCCT GAGGAGGACC 
TTTGTCAACG GAACTCGGAC ACAGCCACCC AGCTGGCTGG AGCTGCAGGC CACCAAGTCC 
AAGAAGCCCA TCATGTTGCC CGTGACCTTC ATGGATGGGA CCACCAAGAC CCTGCTAACA 
GATTCAGCAA CTACAGCCAG GGAGCTGTGC AATGCTCTGG CTGACAAGAT CTCACTCAAG 
GACCGCTTTG GCTTCTCCCT CTACATCGCT CTGTTCGATA AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ATGTCATGGA TGCCATCTCT CAGTGTGAGC AGTACGCCAA GGAGCAGGGT 
GCTCAGGAGC GCAACGCCCC ATGGAGGCTC TTCTTTAGAA AGGAGGTCTT CACACCCTGG 
CACAACCCCT CGGAGGACAA CGTGGCCACG AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCTTCTCAG 
CAGTACTTTG TGGACTATGG TTCTGAGATG ATTCTGGAGC GCCTGCTGAG CCTCGTGCCC 
ACTTACATCC CTGACCGTGA GATCACACCG CTGAAGAATC TTGAGAAGTG GGCACAGCTG 
GCCATTGCTG CCCACAAGAA GGGAATTTAT GCCCAGAGGA GAACTGACTC CCAGAAGGTC 
AAAGAGGATG TGGTCAATTA TGCCCGTTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAC 
GAAGCTTACA AATTCTCAGG CCCTCCCCTC CCCAAGAGCG ACGTCATCGT GGCTGTCAAC 
TGGACGGGTG TGTACTTCGT GGACGAGCAG GAGCAGGTGC TTCTGGAGCT GTCCTTCCCG 
GAGATCATGG CTGTGTCCAG CAGTAGGGAG TGCCGCGTCT TGCTCTCACT GGGCTGCTCT 
GACTTGGGCT GTGCTACTTG TCAATCGGGC CGGGCAGGGC TGACCCCGGC TGGACCCTGT 
TCTCCGTGTT GGTCCTGTAG GGGAACAAAG ATGATGGCCC CCAGCTTTAC CCTGGCCACC 
ATCAAAGGAG ATGAGTACAC CTTCACATCC AGCAATGCTG AGGACATCCG TGACCTGGTG 
GTCACCTTTC TGGAGGGGCT ACGGAAGAGG TCTAAGTATG TGGTGGCACT GCAGGACAAT 
CCTAACCCTG CTGGTGAGGA GTCAGGCTTC CTCAGCTTCG CCAAGGGAGA CCTCATCATC 
CTTGACCATG ATACTGGTGA GCAGGTCATG AACTCAGGCT GGGCCAACGG CATCAACGAG 
AGGACCAAGC AGCGCGGCGA CTTCCCCACT GACTGTGTAT ACGTCATGCC CACTGTCACC 
TTGCCACCAA GGGAGATTGT GGCCCTGGTC ACTATGACCC CAGACCAGAG GCAGGATGTC 
GTCCGGCTCC TGCAGCTTCG CACAGCAGAG CCAGAGGTGC GCGCCAAGCC CTACACGCTA 
GAGGAGTTCT CCTACGACTA CTTCAGGCCC CCACCCAAGC ACACGCTGAG CCGTGTCATG 
GTGTCCAAGG CCCGCGGTAA GGACAGGCTG TGGAGCCACA CACGAGAGCC CCTCAAGCAG 
GCCCTGCTCA AGAAGATCCT GGGCAGTGAA GAACTCTCCC AGGAAGCCTG CATGGCCTTT 
GTAGCTGTGC TCAAGTACAT GGGCGACTAC CCATCCAAGA GGATGCGATC CGTCAATGAG 
CTCACTGACC AGATCTTTGA GTGGGCACTC AAGGCTGAGC CCCTCAAGGA TGAGGCCTAC 
GTGCAGATCC TGAAGCAGCT GACTGACAAT CACATCAGGT ACAGCGAAGA GAGGGGCTGG 
GAACTGCTGT GGCTGTGCAC GGGCCTCTTC CCGCCCAGCA ACATCCTCCT GCCTCATGTT 
CAGCGGTTTC TGCAGTCCCG CAAGCACTGT CCTCTTGCCA TTGACTGCCT GCAGAGGCTC 
CAGAAAGCCC TGAGAAATGG CTCCCGGAAG TACCCTCCGC ACCTGGTGGA GGTGGAGGCC 
ATCCAACATA AGACTACCCA GATCTTCCAC AAGGTCTACT TCCCCGATGA CACGGACGAG 
GCTTTTGAGG TGGAGTCCAG CACCAAGGCC AAGGACTTCT GCCAGAACAT CGCCAGCCGG 
CTGCTGCTCA AGTCTTCCGA GGGATTCAGC CTTTTTGTCA AAATCGCAGA TAAGGTCATC 
AGCGTCCCAG AGAATGATTT CTTCTTTGAC TTTGTCCGAC ACCTGACAGA CTGGATAAAG 
AAAGCACGGC CCATCAAGGA CGGAATCGTG CCCTCACTAA CCTACCAGGT GTTCTTCATG 
AAGAAGCTGT GGACCACCAC AGTGCCGGGC AAGGACCCCA TGGCTGACTC CATCTTCCAC 
TATTACCAGG AACTGCCCAA ATATCTCCGA GGCTACCACA AGTGCACCCG GGAGGAGGTG 
CTGCAGCTGG GCGCACTCAT CTACAGGGTC AAGTTTGAGG AGGACAAATC CTACTTCCCT 
AGCATCCCCA AGTTGCTGAG GGAGCTGGTA CCCCAGGACC TAATCCGGCA GGTCTCACCT 
GATGACTGGA AACGGTCTAT TGTCGCCTAC TTCAACAAAC ATGCGGGGAA GTCCAAGGAG 
GAAGCCAAGC TGGCCTTCCT CAAACTCATC TTCAAGTGGC CCACCTTTGG CTCAGCCTTC 
TTTGAGGTGA AGCAAACTAC AGAACCAAAC TTCCCAGAGA TTCTCTTAAT TGCCATCAAC 
AAGTACGGGG TCAGCCTCAT CGATCCCAGA ACCAAGGACA TCCTGACTAC TCACCCCTTC 
ACCAAGATCT CCAACTGGAG TAGTGGCAAC ACCTACTTCC ACATCACCAT TGGGAACTTG 
GTCCGTGGGA GCAAACTGCT CTGTGAGACA TCGCTGGGAT ACAAAATGGA TGATCTTCTG 
ACTTCCTACA TCAGCCAGAT GCTCACAGCC ATGAGCAAGC AGAGGAACTC CAGGAGTGGA 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_006507428.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507491.1 Mus musculus myosin VIIA (Myo7a), transcript variant X6, mRNA.

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CloneID	OMu39358
Clone ID Related Accession (Same CDS sequence)	XM_006507428.2 , XM_006507428.3
Accession Version	XM_006507428.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACCAGCTGT 
GGAGGACGGA CGTACACAGG TTCCATCCTG GTGGCCGTGA ACCCCTACCA GCTGCTCTCC 
ATCTACTCGC CAGAGCACAT CCGCCAGTAC ACCAACAAGA AGATAGGGGA GATGCCCCCC 
CACATCTTCG CCATTGCTGA CAACTGCTAC TTCAACATGA AACGCAACAA CCGGGACCAG 
TGCTGTATTA TCAGCGGGGA GTCGGGAGCT GGCAAGACAG AGAGCACAAA GTTGATCCTG 
CAGTTCCTGG CAGCCATCAG TGGACAGCAC TCATGGATCG AGCAGCAGGT GCTGGAGGCC 
ACCCCGATCC TGGAAGCATT TGGGAACGCC AAGACCATCC GCAACGACAA CTCTAGCCGC 
TTTGGCAAGT ACATTGACAT CCACTTTAAC AAGCGTGGTG CCATCGAGGG CGCCAAAATA 
GAGCAATACC TGCTGGAGAA GTCACGTGTC TGCCGCCAGG CCCCTGACGA GAGGAACTAT 
CACGTGTTCT ACTGTATGCT GGAGGGCATG AATGAGGAGG AGAAGAAGAA ACTGGGCCTA 
GGCCAGGCCG CTGACTACAA CTACTTGGCC ATGGGTAACT GCATCACCTG TGAGGGCCGC 
GTGGACAGTC AGGAGTATGC CAACATCCGC TCTGCCATGA AGGTTCTCAT GTTCACAGAC 
ACGGAGAACT GGGAGATCTC GAAGCTTCTG GCTGCCATCC TACACATGGG CAATCTGCAG 
TATGAGGCCC GGACATTTGA GAACTTGGAT GCGTGTGAAG TCCTCTTCTC CCCATCGCTG 
GCCACGGCAG CTTCTCTGCT CGAGGTGAAC CCCCCAGACC TGATGAGCTG CCTCACCAGC 
CGCACCCTCA TCACCCGTGG GGAGACGGTG TCCACCCCTC TCAGCAGGGA ACAGGCGCTG 
GATGTGCGAG ATGCCTTTGT CAAGGGCATC TATGGGCGGC TCTTTGTGTG GATTGTGGAG 
AAGATCAACG CAGCAATCTA CAAGCCACCC CCCCTGGAAG TGAAGAACTC TCGCCGGTCC 
ATCGGTCTCC TGGACATCTT TGGATTTGAG AACTTCACTG TGAACAGCTT CGAGCAGCTC 
TGCATTAACT TTGCCAATGA GCACCTGCAG CAATTCTTCG TGCGGCACGT GTTCAAGCTG 
GAGCAGGAGG AGTACGACCT GGAGAGCATC GACTGGTTGC ACATTGAGTT CACTGACAAC 
CAGGAAGCAC TGGACATGAT TGCCAACCGG CCTATGAACG TCATCTCCCT CATCGATGAG 
GAGAGCAAGT TCCCCAAGGG CACGGATGCC ACCATGCTGC ATAAGCTGAA CTCACAGCAC 
AAGCTCAATG CCAACTACGT GCCACCCAAG AACAGCCACG AGACCCAGTT TGGAATCAAC 
CACTTTGCGG GTGTTGTCTA TTATGAGAGT CAAGGCTTCC TGGAGAAGAA CCGAGACACC 
CTGCATGGGG ACATCATCCA GCTGGTCCAC TCTTCCCGGA ACAAGTTCAT AAAGCAGATT 
TTCCAAGCTG ACGTTGCCAT GGGTGCCGAG ACCAGGAAGC GCTCGCCTAC ACTCAGCAGC 
CAGTTCAAGC GGTCTCTGGA GCTGCTGATG CGCACACTGG GCGCCTGCCA GCCCTTCTTT 
GTGCGTTGTA TCAAACCCAA TGAGTTCAAG AAGCCCATGC TCTTCGACCG GCACTTGTGT 
GTACGCCAGC TGCGATATTC GGGCATGATG GAGACAATCC GCATCCGCCA CGCAGGCTAC 
CCCATTCGCT ACAGCTTTGT GGAGTTTGTG GAGCGCTACC GGGTACTGCT GCCTGGTGTG 
AAGCCAGCAT ACAAGCAGGG TGACCTCCGA GGGACATGCC AGCGCATGGC TGAGGCTGTG 
CTGGGCACGC ACGATGACTG GCAGATTGGC AAAACCAAGA TCTTTCTGAA GGACCACCAT 
GACATGTTGC TGGAGGTGGA GCGGGACAAG GCCATCACAG ACAGAGTCAT TCTCCTCCAG 
AAGGTTATCC GGGGCTTCAA AGACAGGTCC AACTTCCTGA GACTGAAGAG TGCTGCCACA 
CTGATCCAGA GGCACTGGCG GGGCCACCAC TGTAGGAAAA ACTATGAGCT GATTCGTCTT 
GGCTTCCTGC GGCTGCAGGC CCTGCACCGC TCCCGGAAGC TGCACAAGCA GTACCGCCTG 
GCCAGACAGC GCATAATAGA GTTCCAGGCC CGCTGCCGGG CCTATCTGGT GCGCAAGGCC 
TTCCGCCACC GCCTCTGGGC CGTGATCACC GTGCAGGCCT ATGCCCGAGG CATGATTGCC 
CGCCGGCTAC ACCGCCGCCT CCGGGTTGAG TACCAGCGGC GCCTCGAGGC AGAGAGGATG 
CGTCTGGCAG AGGAGGAGAA ACTCCGAAAG GAGATGAGTG CCAAGAAGGC CAAAGAGGAG 
GCTGAGCGCA AGCATCAGGA GCGCCTGGCT CAGCTAGCCC GCGAGGATGC GGAGCGGGAA 
CTGAAGGAGA AGGAGGAGGC TCGGAGGAAG AAGGAACTGC TGGAGCAGAT GGAGAAGGCC 
CGCCACGAAC CCATCAACCA CTCAGATATG GTGGACAAGA TGTTTGGCTT CCTGGGGACT 
TCAGGCAGCC TGCCAGGCCA GGAAGGCCAG GCGCCTAGTG GCTTTGAGGA CCTAGAGCGC 
GGACGGAGGG AGATGGTGGA AGAGGATGTT GACGCTGCCC TGCCCCTGCC TGATGAAGAC 
GAGGAGGACC TTTCTGAGTA CAAATTCGCC AAGTTTGCTG CCACCTACTT CCAGGGCACA 
ACCACACACT CCTACACCCG GAGGCCTCTC AAGCAGCCGC TGCTCTACCA CGACGATGAG 
GGTGACCAGC TGGCGGCGCT GGCTGTCTGG ATCACCATCC TCCGGTTCAT GGGGGACCTC 
CCAGAGCCCA AGTACCACAC AGCCATGAGC GACGGCAGTG AGAAGATCCC AGTGATGACT 
AAGATCTACG AGACCCTAGG CAAGAAGACA TATAAGAGGG AGCTGCAGGC CTTGCAGGGC 
GAGGGCGAGA CCCAGCTCCC TGAGGGGCAG AAGAAGACCA GTGTGAGACA CAAGTTGGTA 
CACTTGACAC TGAAGAAAAA GTCCAAACTC ACAGAAGAGG TGACCAAGAG GCTGAACGAT 
GGGGAATCCA CGGTACAGGG CAACAGCATG CTGGAGGATC GGCCCACCTC AAATCTAGAG 
AAGCTGCACT TCATCATCGG CAACGGCATC CTGCGGCCTG CGCTGCGGGA CGAGATTTAC 
TGCCAGATCA GTAAGCAGCT CACACACAAC CCATCCAAGA GCAGCTATGC CAGGGGCTGG 
ATCCTCGTGT CGCTCTGTGT GGGCTGCTTC GCCCCCTCTG AGAAGTTCGT TAAGTACCTG 
CGGAACTTCA TCCACGGAGG CCCACCTGGC TATGCTCCTT ACTGTGAGGA GCGCCTGAGG 
AGGACCTTTG TCAACGGAAC TCGGACACAG CCACCCAGCT GGCTGGAGCT GCAGGCCACC 
AAGTCCAAGA AGCCCATCAT GTTGCCCGTG ACCTTCATGG ATGGGACCAC CAAGACCCTG 
CTAACAGATT CAGCAACTAC AGCCAGGGAG CTGTGCAATG CTCTGGCTGA CAAGATCTCA 
CTCAAGGACC GCTTTGGCTT CTCCCTCTAC ATCGCTCTGT TCGATAAGGT GTCCTCCCTG 
GGCAGCGGCA GTGACCATGT CATGGATGCC ATCTCTCAGT GTGAGCAGTA CGCCAAGGAG 
CAGGGTGCTC AGGAGCGCAA CGCCCCATGG AGGCTCTTCT TTAGAAAGGA GGTCTTCACA 
CCCTGGCACA ACCCCTCGGA GGACAACGTG GCCACGAACC TCATCTACCA GCAGGTGGTG 
CGAGGAGTCA AGTTTGGGGA GTACAGGTGT GAGAAGGAGG ACGACCTGGC TGAGCTGGCT 
TCTCAGCAGT ACTTTGTGGA CTATGGTTCT GAGATGATTC TGGAGCGCCT GCTGAGCCTC 
GTGCCCACTT ACATCCCTGA CCGTGAGATC ACACCGCTGA AGAATCTTGA GAAGTGGGCA 
CAGCTGGCCA TTGCTGCCCA CAAGAAGGGA ATTTATGCCC AGAGGAGAAC TGACTCCCAG 
AAGGTCAAAG AGGATGTGGT CAATTATGCC CGTTTCAAGT GGCCCTTGCT CTTCTCCAGG 
TTTTACGAAG CTTACAAATT CTCAGGCCCT CCCCTCCCCA AGAGCGACGT CATCGTGGCT 
GTCAACTGGA CGGGTGTGTA CTTCGTGGAC GAGCAGGAGC AGGTGCTTCT GGAGCTGTCC 
TTCCCGGAGA TCATGGCTGT GTCCAGCAGT AGGGAGTGCC GCGTCTTGCT CTCACTGGGC 
TGCTCTGACT TGGGCTGTGC TACTTGTCAA TCGGGCCGGG CAGGGCTGAC CCCGGCTGGA 
CCCTGTTCTC CGTGTTGGTC CTGTAGGGGA ACAAAGATGA TGGCCCCCAG CTTTACCCTG 
GCCACCATCA AAGGAGATGA GTACACCTTC ACATCCAGCA ATGCTGAGGA CATCCGTGAC 
CTGGTGGTCA CCTTTCTGGA GGGGCTACGG AAGAGGTCTA AGTATGTGGT GGCACTGCAG 
GACAATCCTA ACCCTGCTGG TGAGGAGTCA GGCTTCCTCA GCTTCGCCAA GGGAGACCTC 
ATCATCCTTG ACCATGATAC TGGTGAGCAG GTCATGAACT CAGGCTGGGC CAACGGCATC 
AACGAGAGGA CCAAGCAGCG CGGCGACTTC CCCACTGACT GTGTATACGT CATGCCCACT 
GTCACCTTGC CACCAAGGGA GATTGTGGCC CTGGTCACTA TGACCCCAGA CCAGAGGCAG 
GATGTCGTCC GGCTCCTGCA GCTTCGCACA GCAGAGCCAG AGGTGCGCGC CAAGCCCTAC 
ACGCTAGAGG AGTTCTCCTA CGACTACTTC AGGCCCCCAC CCAAGCACAC GCTGAGCCGT 
GTCATGGTGT CCAAGGCCCG CGGTAAGGAC AGGCTGTGGA GCCACACACG AGAGCCCCTC 
AAGCAGGCCC TGCTCAAGAA GATCCTGGGC AGTGAAGAAC TCTCCCAGGA AGCCTGCATG 
GCCTTTGTAG CTGTGCTCAA GTACATGGGC GACTACCCAT CCAAGAGGAT GCGATCCGTC 
AATGAGCTCA CTGACCAGAT CTTTGAGTGG GCACTCAAGG CTGAGCCCCT CAAGGATGAG 
GCCTACGTGC AGATCCTGAA GCAGCTGACT GACAATCACA TCAGGTACAG CGAAGAGAGG 
GGCTGGGAAC TGCTGTGGCT GTGCACGGGC CTCTTCCCGC CCAGCAACAT CCTCCTGCCT 
CATGTTCAGC GGTTTCTGCA GTCCCGCAAG CACTGTCCTC TTGCCATTGA CTGCCTGCAG 
AGGCTCCAGA AAGCCCTGAG AAATGGCTCC CGGAAGTACC CTCCGCACCT GGTGGAGGTG 
GAGGCCATCC AACATAAGAC TACCCAGATC TTCCACAAGG TCTACTTCCC CGATGACACG 
GACGAGGCTT TTGAGGTGGA GTCCAGCACC AAGGCCAAGG ACTTCTGCCA GAACATCGCC 
AGCCGGCTGC TGCTCAAGTC TTCCGAGGGA TTCAGCCTTT TTGTCAAAAT CGCAGATAAG 
GTCATCAGCG TCCCAGAGAA TGATTTCTTC TTTGACTTTG TCCGACACCT GACAGACTGG 
ATAAAGAAAG CACGGCCCAT CAAGGACGGA ATCGTGCCCT CACTAACCTA CCAGGTGTTC 
TTCATGAAGA AGCTGTGGAC CACCACAGTG CCGGGCAAGG ACCCCATGGC TGACTCCATC 
TTCCACTATT ACCAGGAACT GCCCAAATAT CTCCGAGGCT ACCACAAGTG CACCCGGGAG 
GAGGTGCTGC AGCTGGGCGC ACTCATCTAC AGGGTCAAGT TTGAGGAGGA CAAATCCTAC 
TTCCCTAGCA TCCCCAAGTT GCTGAGGGAG CTGGTACCCC AGGACCTAAT CCGGCAGGTC 
TCACCTGATG ACTGGAAACG GTCTATTGTC GCCTACTTCA ACAAACATGC GGGGAAGTCC 
AAGGAGGAAG CCAAGCTGGC CTTCCTCAAA CTCATCTTCA AGTGGCCCAC CTTTGGCTCA 
GCCTTCTTTG AGGTGAAGCA AACTACAGAA CCAAACTTCC CAGAGATTCT CTTAATTGCC 
ATCAACAAGT ACGGGGTCAG CCTCATCGAT CCCAGAACCA AGGACATCCT GACTACTCAC 
CCCTTCACCA AGATCTCCAA CTGGAGTAGT GGCAACACCT ACTTCCACAT CACCATTGGG 
AACTTGGTCC GTGGGAGCAA ACTGCTCTGT GAGACATCGC TGGGATACAA AATGGATGAT 
CTTCTGACTT CCTACATCAG CCAGATGCTC ACAGCCATGA GCAAGCAGAG GAACTCCAGG 
AGTGGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507491.1
CDS	209..6700
Translation	MHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_006507428.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507428.2

ORF Insert Sequence:

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACCAGCTGT 
GGAGGACGGA CGTACACAGG TTCCATCCTG GTGGCCGTGA ACCCCTACCA GCTGCTCTCC 
ATCTACTCGC CAGAGCACAT CCGCCAGTAC ACCAACAAGA AGATAGGGGA GATGCCCCCC 
CACATCTTCG CCATTGCTGA CAACTGCTAC TTCAACATGA AACGCAACAA CCGGGACCAG 
TGCTGTATTA TCAGCGGGGA GTCGGGAGCT GGCAAGACAG AGAGCACAAA GTTGATCCTG 
CAGTTCCTGG CAGCCATCAG TGGACAGCAC TCATGGATCG AGCAGCAGGT GCTGGAGGCC 
ACCCCGATCC TGGAAGCATT TGGGAACGCC AAGACCATCC GCAACGACAA CTCTAGCCGC 
TTTGGCAAGT ACATTGACAT CCACTTTAAC AAGCGTGGTG CCATCGAGGG CGCCAAAATA 
GAGCAATACC TGCTGGAGAA GTCACGTGTC TGCCGCCAGG CCCCTGACGA GAGGAACTAT 
CACGTGTTCT ACTGTATGCT GGAGGGCATG AATGAGGAGG AGAAGAAGAA ACTGGGCCTA 
GGCCAGGCCG CTGACTACAA CTACTTGGCC ATGGGTAACT GCATCACCTG TGAGGGCCGC 
GTGGACAGTC AGGAGTATGC CAACATCCGC TCTGCCATGA AGGTTCTCAT GTTCACAGAC 
ACGGAGAACT GGGAGATCTC GAAGCTTCTG GCTGCCATCC TACACATGGG CAATCTGCAG 
TATGAGGCCC GGACATTTGA GAACTTGGAT GCGTGTGAAG TCCTCTTCTC CCCATCGCTG 
GCCACGGCAG CTTCTCTGCT CGAGGTGAAC CCCCCAGACC TGATGAGCTG CCTCACCAGC 
CGCACCCTCA TCACCCGTGG GGAGACGGTG TCCACCCCTC TCAGCAGGGA ACAGGCGCTG 
GATGTGCGAG ATGCCTTTGT CAAGGGCATC TATGGGCGGC TCTTTGTGTG GATTGTGGAG 
AAGATCAACG CAGCAATCTA CAAGCCACCC CCCCTGGAAG TGAAGAACTC TCGCCGGTCC 
ATCGGTCTCC TGGACATCTT TGGATTTGAG AACTTCACTG TGAACAGCTT CGAGCAGCTC 
TGCATTAACT TTGCCAATGA GCACCTGCAG CAATTCTTCG TGCGGCACGT GTTCAAGCTG 
GAGCAGGAGG AGTACGACCT GGAGAGCATC GACTGGTTGC ACATTGAGTT CACTGACAAC 
CAGGAAGCAC TGGACATGAT TGCCAACCGG CCTATGAACG TCATCTCCCT CATCGATGAG 
GAGAGCAAGT TCCCCAAGGG CACGGATGCC ACCATGCTGC ATAAGCTGAA CTCACAGCAC 
AAGCTCAATG CCAACTACGT GCCACCCAAG AACAGCCACG AGACCCAGTT TGGAATCAAC 
CACTTTGCGG GTGTTGTCTA TTATGAGAGT CAAGGCTTCC TGGAGAAGAA CCGAGACACC 
CTGCATGGGG ACATCATCCA GCTGGTCCAC TCTTCCCGGA ACAAGTTCAT AAAGCAGATT 
TTCCAAGCTG ACGTTGCCAT GGGTGCCGAG ACCAGGAAGC GCTCGCCTAC ACTCAGCAGC 
CAGTTCAAGC GGTCTCTGGA GCTGCTGATG CGCACACTGG GCGCCTGCCA GCCCTTCTTT 
GTGCGTTGTA TCAAACCCAA TGAGTTCAAG AAGCCCATGC TCTTCGACCG GCACTTGTGT 
GTACGCCAGC TGCGATATTC GGGCATGATG GAGACAATCC GCATCCGCCA CGCAGGCTAC 
CCCATTCGCT ACAGCTTTGT GGAGTTTGTG GAGCGCTACC GGGTACTGCT GCCTGGTGTG 
AAGCCAGCAT ACAAGCAGGG TGACCTCCGA GGGACATGCC AGCGCATGGC TGAGGCTGTG 
CTGGGCACGC ACGATGACTG GCAGATTGGC AAAACCAAGA TCTTTCTGAA GGACCACCAT 
GACATGTTGC TGGAGGTGGA GCGGGACAAG GCCATCACAG ACAGAGTCAT TCTCCTCCAG 
AAGGTTATCC GGGGCTTCAA AGACAGGTCC AACTTCCTGA GACTGAAGAG TGCTGCCACA 
CTGATCCAGA GGCACTGGCG GGGCCACCAC TGTAGGAAAA ACTATGAGCT GATTCGTCTT 
GGCTTCCTGC GGCTGCAGGC CCTGCACCGC TCCCGGAAGC TGCACAAGCA GTACCGCCTG 
GCCAGACAGC GCATAATAGA GTTCCAGGCC CGCTGCCGGG CCTATCTGGT GCGCAAGGCC 
TTCCGCCACC GCCTCTGGGC CGTGATCACC GTGCAGGCCT ATGCCCGAGG CATGATTGCC 
CGCCGGCTAC ACCGCCGCCT CCGGGTTGAG TACCAGCGGC GCCTCGAGGC AGAGAGGATG 
CGTCTGGCAG AGGAGGAGAA ACTCCGAAAG GAGATGAGTG CCAAGAAGGC CAAAGAGGAG 
GCTGAGCGCA AGCATCAGGA GCGCCTGGCT CAGCTAGCCC GCGAGGATGC GGAGCGGGAA 
CTGAAGGAGA AGGAGGAGGC TCGGAGGAAG AAGGAACTGC TGGAGCAGAT GGAGAAGGCC 
CGCCACGAAC CCATCAACCA CTCAGATATG GTGGACAAGA TGTTTGGCTT CCTGGGGACT 
TCAGGCAGCC TGCCAGGCCA GGAAGGCCAG GCGCCTAGTG GCTTTGAGGA CCTAGAGCGC 
GGACGGAGGG AGATGGTGGA AGAGGATGTT GACGCTGCCC TGCCCCTGCC TGATGAAGAC 
GAGGAGGACC TTTCTGAGTA CAAATTCGCC AAGTTTGCTG CCACCTACTT CCAGGGCACA 
ACCACACACT CCTACACCCG GAGGCCTCTC AAGCAGCCGC TGCTCTACCA CGACGATGAG 
GGTGACCAGC TGGCGGCGCT GGCTGTCTGG ATCACCATCC TCCGGTTCAT GGGGGACCTC 
CCAGAGCCCA AGTACCACAC AGCCATGAGC GACGGCAGTG AGAAGATCCC AGTGATGACT 
AAGATCTACG AGACCCTAGG CAAGAAGACA TATAAGAGGG AGCTGCAGGC CTTGCAGGGC 
GAGGGCGAGA CCCAGCTCCC TGAGGGGCAG AAGAAGACCA GTGTGAGACA CAAGTTGGTA 
CACTTGACAC TGAAGAAAAA GTCCAAACTC ACAGAAGAGG TGACCAAGAG GCTGAACGAT 
GGGGAATCCA CGGTACAGGG CAACAGCATG CTGGAGGATC GGCCCACCTC AAATCTAGAG 
AAGCTGCACT TCATCATCGG CAACGGCATC CTGCGGCCTG CGCTGCGGGA CGAGATTTAC 
TGCCAGATCA GTAAGCAGCT CACACACAAC CCATCCAAGA GCAGCTATGC CAGGGGCTGG 
ATCCTCGTGT CGCTCTGTGT GGGCTGCTTC GCCCCCTCTG AGAAGTTCGT TAAGTACCTG 
CGGAACTTCA TCCACGGAGG CCCACCTGGC TATGCTCCTT ACTGTGAGGA GCGCCTGAGG 
AGGACCTTTG TCAACGGAAC TCGGACACAG CCACCCAGCT GGCTGGAGCT GCAGGCCACC 
AAGTCCAAGA AGCCCATCAT GTTGCCCGTG ACCTTCATGG ATGGGACCAC CAAGACCCTG 
CTAACAGATT CAGCAACTAC AGCCAGGGAG CTGTGCAATG CTCTGGCTGA CAAGATCTCA 
CTCAAGGACC GCTTTGGCTT CTCCCTCTAC ATCGCTCTGT TCGATAAGGT GTCCTCCCTG 
GGCAGCGGCA GTGACCATGT CATGGATGCC ATCTCTCAGT GTGAGCAGTA CGCCAAGGAG 
CAGGGTGCTC AGGAGCGCAA CGCCCCATGG AGGCTCTTCT TTAGAAAGGA GGTCTTCACA 
CCCTGGCACA ACCCCTCGGA GGACAACGTG GCCACGAACC TCATCTACCA GCAGGTGGTG 
CGAGGAGTCA AGTTTGGGGA GTACAGGTGT GAGAAGGAGG ACGACCTGGC TGAGCTGGCT 
TCTCAGCAGT ACTTTGTGGA CTATGGTTCT GAGATGATTC TGGAGCGCCT GCTGAGCCTC 
GTGCCCACTT ACATCCCTGA CCGTGAGATC ACACCGCTGA AGAATCTTGA GAAGTGGGCA 
CAGCTGGCCA TTGCTGCCCA CAAGAAGGGA ATTTATGCCC AGAGGAGAAC TGACTCCCAG 
AAGGTCAAAG AGGATGTGGT CAATTATGCC CGTTTCAAGT GGCCCTTGCT CTTCTCCAGG 
TTTTACGAAG CTTACAAATT CTCAGGCCCT CCCCTCCCCA AGAGCGACGT CATCGTGGCT 
GTCAACTGGA CGGGTGTGTA CTTCGTGGAC GAGCAGGAGC AGGTGCTTCT GGAGCTGTCC 
TTCCCGGAGA TCATGGCTGT GTCCAGCAGT AGGGAGTGCC GCGTCTTGCT CTCACTGGGC 
TGCTCTGACT TGGGCTGTGC TACTTGTCAA TCGGGCCGGG CAGGGCTGAC CCCGGCTGGA 
CCCTGTTCTC CGTGTTGGTC CTGTAGGGGA ACAAAGATGA TGGCCCCCAG CTTTACCCTG 
GCCACCATCA AAGGAGATGA GTACACCTTC ACATCCAGCA ATGCTGAGGA CATCCGTGAC 
CTGGTGGTCA CCTTTCTGGA GGGGCTACGG AAGAGGTCTA AGTATGTGGT GGCACTGCAG 
GACAATCCTA ACCCTGCTGG TGAGGAGTCA GGCTTCCTCA GCTTCGCCAA GGGAGACCTC 
ATCATCCTTG ACCATGATAC TGGTGAGCAG GTCATGAACT CAGGCTGGGC CAACGGCATC 
AACGAGAGGA CCAAGCAGCG CGGCGACTTC CCCACTGACT GTGTATACGT CATGCCCACT 
GTCACCTTGC CACCAAGGGA GATTGTGGCC CTGGTCACTA TGACCCCAGA CCAGAGGCAG 
GATGTCGTCC GGCTCCTGCA GCTTCGCACA GCAGAGCCAG AGGTGCGCGC CAAGCCCTAC 
ACGCTAGAGG AGTTCTCCTA CGACTACTTC AGGCCCCCAC CCAAGCACAC GCTGAGCCGT 
GTCATGGTGT CCAAGGCCCG CGGTAAGGAC AGGCTGTGGA GCCACACACG AGAGCCCCTC 
AAGCAGGCCC TGCTCAAGAA GATCCTGGGC AGTGAAGAAC TCTCCCAGGA AGCCTGCATG 
GCCTTTGTAG CTGTGCTCAA GTACATGGGC GACTACCCAT CCAAGAGGAT GCGATCCGTC 
AATGAGCTCA CTGACCAGAT CTTTGAGTGG GCACTCAAGG CTGAGCCCCT CAAGGATGAG 
GCCTACGTGC AGATCCTGAA GCAGCTGACT GACAATCACA TCAGGTACAG CGAAGAGAGG 
GGCTGGGAAC TGCTGTGGCT GTGCACGGGC CTCTTCCCGC CCAGCAACAT CCTCCTGCCT 
CATGTTCAGC GGTTTCTGCA GTCCCGCAAG CACTGTCCTC TTGCCATTGA CTGCCTGCAG 
AGGCTCCAGA AAGCCCTGAG AAATGGCTCC CGGAAGTACC CTCCGCACCT GGTGGAGGTG 
GAGGCCATCC AACATAAGAC TACCCAGATC TTCCACAAGG TCTACTTCCC CGATGACACG 
GACGAGGCTT TTGAGGTGGA GTCCAGCACC AAGGCCAAGG ACTTCTGCCA GAACATCGCC 
AGCCGGCTGC TGCTCAAGTC TTCCGAGGGA TTCAGCCTTT TTGTCAAAAT CGCAGATAAG 
GTCATCAGCG TCCCAGAGAA TGATTTCTTC TTTGACTTTG TCCGACACCT GACAGACTGG 
ATAAAGAAAG CACGGCCCAT CAAGGACGGA ATCGTGCCCT CACTAACCTA CCAGGTGTTC 
TTCATGAAGA AGCTGTGGAC CACCACAGTG CCGGGCAAGG ACCCCATGGC TGACTCCATC 
TTCCACTATT ACCAGGAACT GCCCAAATAT CTCCGAGGCT ACCACAAGTG CACCCGGGAG 
GAGGTGCTGC AGCTGGGCGC ACTCATCTAC AGGGTCAAGT TTGAGGAGGA CAAATCCTAC 
TTCCCTAGCA TCCCCAAGTT GCTGAGGGAG CTGGTACCCC AGGACCTAAT CCGGCAGGTC 
TCACCTGATG ACTGGAAACG GTCTATTGTC GCCTACTTCA ACAAACATGC GGGGAAGTCC 
AAGGAGGAAG CCAAGCTGGC CTTCCTCAAA CTCATCTTCA AGTGGCCCAC CTTTGGCTCA 
GCCTTCTTTG AGGTGAAGCA AACTACAGAA CCAAACTTCC CAGAGATTCT CTTAATTGCC 
ATCAACAAGT ACGGGGTCAG CCTCATCGAT CCCAGAACCA AGGACATCCT GACTACTCAC 
CCCTTCACCA AGATCTCCAA CTGGAGTAGT GGCAACACCT ACTTCCACAT CACCATTGGG 
AACTTGGTCC GTGGGAGCAA ACTGCTCTGT GAGACATCGC TGGGATACAA AATGGATGAT 
CTTCTGACTT CCTACATCAG CCAGATGCTC ACAGCCATGA GCAAGCAGAG GAACTCCAGG 
AGTGGAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_006507429.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507492.1 Mus musculus myosin VIIA (Myo7a), transcript variant X7, mRNA.

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CloneID	OMu39359
Clone ID Related Accession (Same CDS sequence)	XM_006507429.2 , XM_006507429.3
Accession Version	XM_006507429.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACGTACACA 
GGTTCCATCC TGGTGGCCGT GAACCCCTAC CAGCTGCTCT CCATCTACTC GCCAGAGCAC 
ATCCGCCAGT ACACCAACAA GAAGATAGGG GAGATGCCCC CCCACATCTT CGCCATTGCT 
GACAACTGCT ACTTCAACAT GAAACGCAAC AACCGGGACC AGTGCTGTAT TATCAGCGGG 
GAGTCGGGAG CTGGCAAGAC AGAGAGCACA AAGTTGATCC TGCAGTTCCT GGCAGCCATC 
AGTGGACAGC ACTCATGGAT CGAGCAGCAG GTGCTGGAGG CCACCCCGAT CCTGGAAGCA 
TTTGGGAACG CCAAGACCAT CCGCAACGAC AACTCTAGCC GCTTTGGCAA GTACATTGAC 
ATCCACTTTA ACAAGCGTGG TGCCATCGAG GGCGCCAAAA TAGAGCAATA CCTGCTGGAG 
AAGTCACGTG TCTGCCGCCA GGCCCCTGAC GAGAGGAACT ATCACGTGTT CTACTGTATG 
CTGGAGGGCA TGAATGAGGA GGAGAAGAAG AAACTGGGCC TAGGCCAGGC CGCTGACTAC 
AACTACTTGG CCATGGGTAA CTGCATCACC TGTGAGGGCC GCGTGGACAG TCAGGAGTAT 
GCCAACATCC GCTCTGCCAT GAAGGTTCTC ATGTTCACAG ACACGGAGAA CTGGGAGATC 
TCGAAGCTTC TGGCTGCCAT CCTACACATG GGCAATCTGC AGTATGAGGC CCGGACATTT 
GAGAACTTGG ATGCGTGTGA AGTCCTCTTC TCCCCATCGC TGGCCACGGC AGCTTCTCTG 
CTCGAGGTGA ACCCCCCAGA CCTGATGAGC TGCCTCACCA GCCGCACCCT CATCACCCGT 
GGGGAGACGG TGTCCACCCC TCTCAGCAGG GAACAGGCGC TGGATGTGCG AGATGCCTTT 
GTCAAGGGCA TCTATGGGCG GCTCTTTGTG TGGATTGTGG AGAAGATCAA CGCAGCAATC 
TACAAGCCAC CCCCCCTGGA AGTGAAGAAC TCTCGCCGGT CCATCGGTCT CCTGGACATC 
TTTGGATTTG AGAACTTCAC TGTGAACAGC TTCGAGCAGC TCTGCATTAA CTTTGCCAAT 
GAGCACCTGC AGCAATTCTT CGTGCGGCAC GTGTTCAAGC TGGAGCAGGA GGAGTACGAC 
CTGGAGAGCA TCGACTGGTT GCACATTGAG TTCACTGACA ACCAGGAAGC ACTGGACATG 
ATTGCCAACC GGCCTATGAA CGTCATCTCC CTCATCGATG AGGAGAGCAA GTTCCCCAAG 
GGCACGGATG CCACCATGCT GCATAAGCTG AACTCACAGC ACAAGCTCAA TGCCAACTAC 
GTGCCACCCA AGAACAGCCA CGAGACCCAG TTTGGAATCA ACCACTTTGC GGGTGTTGTC 
TATTATGAGA GTCAAGGCTT CCTGGAGAAG AACCGAGACA CCCTGCATGG GGACATCATC 
CAGCTGGTCC ACTCTTCCCG GAACAAGTTC ATAAAGCAGA TTTTCCAAGC TGACGTTGCC 
ATGGGTGCCG AGACCAGGAA GCGCTCGCCT ACACTCAGCA GCCAGTTCAA GCGGTCTCTG 
GAGCTGCTGA TGCGCACACT GGGCGCCTGC CAGCCCTTCT TTGTGCGTTG TATCAAACCC 
AATGAGTTCA AGAAGCCCAT GCTCTTCGAC CGGCACTTGT GTGTACGCCA GCTGCGATAT 
TCGGGCATGA TGGAGACAAT CCGCATCCGC CACGCAGGCT ACCCCATTCG CTACAGCTTT 
GTGGAGTTTG TGGAGCGCTA CCGGGTACTG CTGCCTGGTG TGAAGCCAGC ATACAAGCAG 
GGTGACCTCC GAGGGACATG CCAGCGCATG GCTGAGGCTG TGCTGGGCAC GCACGATGAC 
TGGCAGATTG GCAAAACCAA GATCTTTCTG AAGGACCACC ATGACATGTT GCTGGAGGTG 
GAGCGGGACA AGGCCATCAC AGACAGAGTC ATTCTCCTCC AGAAGGTTAT CCGGGGCTTC 
AAAGACAGGT CCAACTTCCT GAGACTGAAG AGTGCTGCCA CACTGATCCA GAGGCACTGG 
CGGGGCCACC ACTGTAGGAA AAACTATGAG CTGATTCGTC TTGGCTTCCT GCGGCTGCAG 
GCCCTGCACC GCTCCCGGAA GCTGCACAAG CAGTACCGCC TGGCCAGACA GCGCATAATA 
GAGTTCCAGG CCCGCTGCCG GGCCTATCTG GTGCGCAAGG CCTTCCGCCA CCGCCTCTGG 
GCCGTGATCA CCGTGCAGGC CTATGCCCGA GGCATGATTG CCCGCCGGCT ACACCGCCGC 
CTCCGGGTTG AGTACCAGCG GCGCCTCGAG GCAGAGAGGA TGCGTCTGGC AGAGGAGGAG 
AAACTCCGAA AGGAGATGAG TGCCAAGAAG GCCAAAGAGG AGGCTGAGCG CAAGCATCAG 
GAGCGCCTGG CTCAGCTAGC CCGCGAGGAT GCGGAGCGGG AACTGAAGGA GAAGGAGGAG 
GCTCGGAGGA AGAAGGAACT GCTGGAGCAG ATGGAGAAGG CCCGCCACGA ACCCATCAAC 
CACTCAGATA TGGTGGACAA GATGTTTGGC TTCCTGGGGA CTTCAGGCAG CCTGCCAGGC 
CAGGAAGGCC AGGCGCCTAG TGGCTTTGAG GACCTAGAGC GCGGACGGAG GGAGATGGTG 
GAAGAGGATG TTGACGCTGC CCTGCCCCTG CCTGATGAAG ACGAGGAGGA CCTTTCTGAG 
TACAAATTCG CCAAGTTTGC TGCCACCTAC TTCCAGGGCA CAACCACACA CTCCTACACC 
CGGAGGCCTC TCAAGCAGCC GCTGCTCTAC CACGACGATG AGGGTGACCA GCTGGCGGCG 
CTGGCTGTCT GGATCACCAT CCTCCGGTTC ATGGGGGACC TCCCAGAGCC CAAGTACCAC 
ACAGCCATGA GCGACGGCAG TGAGAAGATC CCAGTGATGA CTAAGATCTA CGAGACCCTA 
GGCAAGAAGA CATATAAGAG GGAGCTGCAG GCCTTGCAGG GCGAGGGCGA GACCCAGCTC 
CCTGAGGGGC AGAAGAAGAC CAGTGTGAGA CACAAGTTGG TACACTTGAC ACTGAAGAAA 
AAGTCCAAAC TCACAGAAGA GGTGACCAAG AGGCTGAACG ATGGGGAATC CACGGTACAG 
GGCAACAGCA TGCTGGAGGA TCGGCCCACC TCAAATCTAG AGAAGCTGCA CTTCATCATC 
GGCAACGGCA TCCTGCGGCC TGCGCTGCGG GACGAGATTT ACTGCCAGAT CAGTAAGCAG 
CTCACACACA ACCCATCCAA GAGCAGCTAT GCCAGGGGCT GGATCCTCGT GTCGCTCTGT 
GTGGGCTGCT TCGCCCCCTC TGAGAAGTTC GTTAAGTACC TGCGGAACTT CATCCACGGA 
GGCCCACCTG GCTATGCTCC TTACTGTGAG GAGCGCCTGA GGAGGACCTT TGTCAACGGA 
ACTCGGACAC AGCCACCCAG CTGGCTGGAG CTGCAGGCCA CCAAGTCCAA GAAGCCCATC 
ATGTTGCCCG TGACCTTCAT GGATGGGACC ACCAAGACCC TGCTAACAGA TTCAGCAACT 
ACAGCCAGGG AGCTGTGCAA TGCTCTGGCT GACAAGATCT CACTCAAGGA CCGCTTTGGC 
TTCTCCCTCT ACATCGCTCT GTTCGATAAG GTGTCCTCCC TGGGCAGCGG CAGTGACCAT 
GTCATGGATG CCATCTCTCA GTGTGAGCAG TACGCCAAGG AGCAGGGTGC TCAGGAGCGC 
AACGCCCCAT GGAGGCTCTT CTTTAGAAAG GAGGTCTTCA CACCCTGGCA CAACCCCTCG 
GAGGACAACG TGGCCACGAA CCTCATCTAC CAGCAGGTGG TGCGAGGAGT CAAGTTTGGG 
GAGTACAGGT GTGAGAAGGA GGACGACCTG GCTGAGCTGG CTTCTCAGCA GTACTTTGTG 
GACTATGGTT CTGAGATGAT TCTGGAGCGC CTGCTGAGCC TCGTGCCCAC TTACATCCCT 
GACCGTGAGA TCACACCGCT GAAGAATCTT GAGAAGTGGG CACAGCTGGC CATTGCTGCC 
CACAAGAAGG GAATTTATGC CCAGAGGAGA ACTGACTCCC AGAAGGTCAA AGAGGATGTG 
GTCAATTATG CCCGTTTCAA GTGGCCCTTG CTCTTCTCCA GGTTTTACGA AGCTTACAAA 
TTCTCAGGCC CTCCCCTCCC CAAGAGCGAC GTCATCGTGG CTGTCAACTG GACGGGTGTG 
TACTTCGTGG ACGAGCAGGA GCAGGTGCTT CTGGAGCTGT CCTTCCCGGA GATCATGGCT 
GTGTCCAGCA GTAGGGAGTG CCGCGTCTTG CTCTCACTGG GCTGCTCTGA CTTGGGCTGT 
GCTACTTGTC AATCGGGCCG GGCAGGGCTG ACCCCGGCTG GACCCTGTTC TCCGTGTTGG 
TCCTGTAGGG GAACAAAGAT GATGGCCCCC AGCTTTACCC TGGCCACCAT CAAAGGAGAT 
GAGTACACCT TCACATCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTTCTG 
GAGGGGCTAC GGAAGAGGTC TAAGTATGTG GTGGCACTGC AGGACAATCC TAACCCTGCT 
GGTGAGGAGT CAGGCTTCCT CAGCTTCGCC AAGGGAGACC TCATCATCCT TGACCATGAT 
ACTGGTGAGC AGGTCATGAA CTCAGGCTGG GCCAACGGCA TCAACGAGAG GACCAAGCAG 
CGCGGCGACT TCCCCACTGA CTGTGTATAC GTCATGCCCA CTGTCACCTT GCCACCAAGG 
GAGATTGTGG CCCTGGTCAC TATGACCCCA GACCAGAGGC AGGATGTCGT CCGGCTCCTG 
CAGCTTCGCA CAGCAGAGCC AGAGGTGCGC GCCAAGCCCT ACACGCTAGA GGAGTTCTCC 
TACGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGCGGTAAGG ACAGGCTGTG GAGCCACACA CGAGAGCCCC TCAAGCAGGC CCTGCTCAAG 
AAGATCCTGG GCAGTGAAGA ACTCTCCCAG GAAGCCTGCA TGGCCTTTGT AGCTGTGCTC 
AAGTACATGG GCGACTACCC ATCCAAGAGG ATGCGATCCG TCAATGAGCT CACTGACCAG 
ATCTTTGAGT GGGCACTCAA GGCTGAGCCC CTCAAGGATG AGGCCTACGT GCAGATCCTG 
AAGCAGCTGA CTGACAATCA CATCAGGTAC AGCGAAGAGA GGGGCTGGGA ACTGCTGTGG 
CTGTGCACGG GCCTCTTCCC GCCCAGCAAC ATCCTCCTGC CTCATGTTCA GCGGTTTCTG 
CAGTCCCGCA AGCACTGTCC TCTTGCCATT GACTGCCTGC AGAGGCTCCA GAAAGCCCTG 
AGAAATGGCT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAACATAAG 
ACTACCCAGA TCTTCCACAA GGTCTACTTC CCCGATGACA CGGACGAGGC TTTTGAGGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCAGCCGGCT GCTGCTCAAG 
TCTTCCGAGG GATTCAGCCT TTTTGTCAAA ATCGCAGATA AGGTCATCAG CGTCCCAGAG 
AATGATTTCT TCTTTGACTT TGTCCGACAC CTGACAGACT GGATAAAGAA AGCACGGCCC 
ATCAAGGACG GAATCGTGCC CTCACTAACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACAG TGCCGGGCAA GGACCCCATG GCTGACTCCA TCTTCCACTA TTACCAGGAA 
CTGCCCAAAT ATCTCCGAGG CTACCACAAG TGCACCCGGG AGGAGGTGCT GCAGCTGGGC 
GCACTCATCT ACAGGGTCAA GTTTGAGGAG GACAAATCCT ACTTCCCTAG CATCCCCAAG 
TTGCTGAGGG AGCTGGTACC CCAGGACCTA ATCCGGCAGG TCTCACCTGA TGACTGGAAA 
CGGTCTATTG TCGCCTACTT CAACAAACAT GCGGGGAAGT CCAAGGAGGA AGCCAAGCTG 
GCCTTCCTCA AACTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT TGAGGTGAAG 
CAAACTACAG AACCAAACTT CCCAGAGATT CTCTTAATTG CCATCAACAA GTACGGGGTC 
AGCCTCATCG ATCCCAGAAC CAAGGACATC CTGACTACTC ACCCCTTCAC CAAGATCTCC 
AACTGGAGTA GTGGCAACAC CTACTTCCAC ATCACCATTG GGAACTTGGT CCGTGGGAGC 
AAACTGCTCT GTGAGACATC GCTGGGATAC AAAATGGATG ATCTTCTGAC TTCCTACATC 
AGCCAGATGC TCACAGCCAT GAGCAAGCAG AGGAACTCCA GGAGTGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507492.1
CDS	213..6686
Translation	MHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_006507429.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507429.2

ORF Insert Sequence:

ATGCACCCCA CATCGGTGCA CGGCGTGGAG GACATGATCC GCCTGGGGGA TCTCAACGAG 
GCAGGCATCC TTCGAAACCT TCTCATTCGC TACCGGGACC ACCTCATCTA TACGTACACA 
GGTTCCATCC TGGTGGCCGT GAACCCCTAC CAGCTGCTCT CCATCTACTC GCCAGAGCAC 
ATCCGCCAGT ACACCAACAA GAAGATAGGG GAGATGCCCC CCCACATCTT CGCCATTGCT 
GACAACTGCT ACTTCAACAT GAAACGCAAC AACCGGGACC AGTGCTGTAT TATCAGCGGG 
GAGTCGGGAG CTGGCAAGAC AGAGAGCACA AAGTTGATCC TGCAGTTCCT GGCAGCCATC 
AGTGGACAGC ACTCATGGAT CGAGCAGCAG GTGCTGGAGG CCACCCCGAT CCTGGAAGCA 
TTTGGGAACG CCAAGACCAT CCGCAACGAC AACTCTAGCC GCTTTGGCAA GTACATTGAC 
ATCCACTTTA ACAAGCGTGG TGCCATCGAG GGCGCCAAAA TAGAGCAATA CCTGCTGGAG 
AAGTCACGTG TCTGCCGCCA GGCCCCTGAC GAGAGGAACT ATCACGTGTT CTACTGTATG 
CTGGAGGGCA TGAATGAGGA GGAGAAGAAG AAACTGGGCC TAGGCCAGGC CGCTGACTAC 
AACTACTTGG CCATGGGTAA CTGCATCACC TGTGAGGGCC GCGTGGACAG TCAGGAGTAT 
GCCAACATCC GCTCTGCCAT GAAGGTTCTC ATGTTCACAG ACACGGAGAA CTGGGAGATC 
TCGAAGCTTC TGGCTGCCAT CCTACACATG GGCAATCTGC AGTATGAGGC CCGGACATTT 
GAGAACTTGG ATGCGTGTGA AGTCCTCTTC TCCCCATCGC TGGCCACGGC AGCTTCTCTG 
CTCGAGGTGA ACCCCCCAGA CCTGATGAGC TGCCTCACCA GCCGCACCCT CATCACCCGT 
GGGGAGACGG TGTCCACCCC TCTCAGCAGG GAACAGGCGC TGGATGTGCG AGATGCCTTT 
GTCAAGGGCA TCTATGGGCG GCTCTTTGTG TGGATTGTGG AGAAGATCAA CGCAGCAATC 
TACAAGCCAC CCCCCCTGGA AGTGAAGAAC TCTCGCCGGT CCATCGGTCT CCTGGACATC 
TTTGGATTTG AGAACTTCAC TGTGAACAGC TTCGAGCAGC TCTGCATTAA CTTTGCCAAT 
GAGCACCTGC AGCAATTCTT CGTGCGGCAC GTGTTCAAGC TGGAGCAGGA GGAGTACGAC 
CTGGAGAGCA TCGACTGGTT GCACATTGAG TTCACTGACA ACCAGGAAGC ACTGGACATG 
ATTGCCAACC GGCCTATGAA CGTCATCTCC CTCATCGATG AGGAGAGCAA GTTCCCCAAG 
GGCACGGATG CCACCATGCT GCATAAGCTG AACTCACAGC ACAAGCTCAA TGCCAACTAC 
GTGCCACCCA AGAACAGCCA CGAGACCCAG TTTGGAATCA ACCACTTTGC GGGTGTTGTC 
TATTATGAGA GTCAAGGCTT CCTGGAGAAG AACCGAGACA CCCTGCATGG GGACATCATC 
CAGCTGGTCC ACTCTTCCCG GAACAAGTTC ATAAAGCAGA TTTTCCAAGC TGACGTTGCC 
ATGGGTGCCG AGACCAGGAA GCGCTCGCCT ACACTCAGCA GCCAGTTCAA GCGGTCTCTG 
GAGCTGCTGA TGCGCACACT GGGCGCCTGC CAGCCCTTCT TTGTGCGTTG TATCAAACCC 
AATGAGTTCA AGAAGCCCAT GCTCTTCGAC CGGCACTTGT GTGTACGCCA GCTGCGATAT 
TCGGGCATGA TGGAGACAAT CCGCATCCGC CACGCAGGCT ACCCCATTCG CTACAGCTTT 
GTGGAGTTTG TGGAGCGCTA CCGGGTACTG CTGCCTGGTG TGAAGCCAGC ATACAAGCAG 
GGTGACCTCC GAGGGACATG CCAGCGCATG GCTGAGGCTG TGCTGGGCAC GCACGATGAC 
TGGCAGATTG GCAAAACCAA GATCTTTCTG AAGGACCACC ATGACATGTT GCTGGAGGTG 
GAGCGGGACA AGGCCATCAC AGACAGAGTC ATTCTCCTCC AGAAGGTTAT CCGGGGCTTC 
AAAGACAGGT CCAACTTCCT GAGACTGAAG AGTGCTGCCA CACTGATCCA GAGGCACTGG 
CGGGGCCACC ACTGTAGGAA AAACTATGAG CTGATTCGTC TTGGCTTCCT GCGGCTGCAG 
GCCCTGCACC GCTCCCGGAA GCTGCACAAG CAGTACCGCC TGGCCAGACA GCGCATAATA 
GAGTTCCAGG CCCGCTGCCG GGCCTATCTG GTGCGCAAGG CCTTCCGCCA CCGCCTCTGG 
GCCGTGATCA CCGTGCAGGC CTATGCCCGA GGCATGATTG CCCGCCGGCT ACACCGCCGC 
CTCCGGGTTG AGTACCAGCG GCGCCTCGAG GCAGAGAGGA TGCGTCTGGC AGAGGAGGAG 
AAACTCCGAA AGGAGATGAG TGCCAAGAAG GCCAAAGAGG AGGCTGAGCG CAAGCATCAG 
GAGCGCCTGG CTCAGCTAGC CCGCGAGGAT GCGGAGCGGG AACTGAAGGA GAAGGAGGAG 
GCTCGGAGGA AGAAGGAACT GCTGGAGCAG ATGGAGAAGG CCCGCCACGA ACCCATCAAC 
CACTCAGATA TGGTGGACAA GATGTTTGGC TTCCTGGGGA CTTCAGGCAG CCTGCCAGGC 
CAGGAAGGCC AGGCGCCTAG TGGCTTTGAG GACCTAGAGC GCGGACGGAG GGAGATGGTG 
GAAGAGGATG TTGACGCTGC CCTGCCCCTG CCTGATGAAG ACGAGGAGGA CCTTTCTGAG 
TACAAATTCG CCAAGTTTGC TGCCACCTAC TTCCAGGGCA CAACCACACA CTCCTACACC 
CGGAGGCCTC TCAAGCAGCC GCTGCTCTAC CACGACGATG AGGGTGACCA GCTGGCGGCG 
CTGGCTGTCT GGATCACCAT CCTCCGGTTC ATGGGGGACC TCCCAGAGCC CAAGTACCAC 
ACAGCCATGA GCGACGGCAG TGAGAAGATC CCAGTGATGA CTAAGATCTA CGAGACCCTA 
GGCAAGAAGA CATATAAGAG GGAGCTGCAG GCCTTGCAGG GCGAGGGCGA GACCCAGCTC 
CCTGAGGGGC AGAAGAAGAC CAGTGTGAGA CACAAGTTGG TACACTTGAC ACTGAAGAAA 
AAGTCCAAAC TCACAGAAGA GGTGACCAAG AGGCTGAACG ATGGGGAATC CACGGTACAG 
GGCAACAGCA TGCTGGAGGA TCGGCCCACC TCAAATCTAG AGAAGCTGCA CTTCATCATC 
GGCAACGGCA TCCTGCGGCC TGCGCTGCGG GACGAGATTT ACTGCCAGAT CAGTAAGCAG 
CTCACACACA ACCCATCCAA GAGCAGCTAT GCCAGGGGCT GGATCCTCGT GTCGCTCTGT 
GTGGGCTGCT TCGCCCCCTC TGAGAAGTTC GTTAAGTACC TGCGGAACTT CATCCACGGA 
GGCCCACCTG GCTATGCTCC TTACTGTGAG GAGCGCCTGA GGAGGACCTT TGTCAACGGA 
ACTCGGACAC AGCCACCCAG CTGGCTGGAG CTGCAGGCCA CCAAGTCCAA GAAGCCCATC 
ATGTTGCCCG TGACCTTCAT GGATGGGACC ACCAAGACCC TGCTAACAGA TTCAGCAACT 
ACAGCCAGGG AGCTGTGCAA TGCTCTGGCT GACAAGATCT CACTCAAGGA CCGCTTTGGC 
TTCTCCCTCT ACATCGCTCT GTTCGATAAG GTGTCCTCCC TGGGCAGCGG CAGTGACCAT 
GTCATGGATG CCATCTCTCA GTGTGAGCAG TACGCCAAGG AGCAGGGTGC TCAGGAGCGC 
AACGCCCCAT GGAGGCTCTT CTTTAGAAAG GAGGTCTTCA CACCCTGGCA CAACCCCTCG 
GAGGACAACG TGGCCACGAA CCTCATCTAC CAGCAGGTGG TGCGAGGAGT CAAGTTTGGG 
GAGTACAGGT GTGAGAAGGA GGACGACCTG GCTGAGCTGG CTTCTCAGCA GTACTTTGTG 
GACTATGGTT CTGAGATGAT TCTGGAGCGC CTGCTGAGCC TCGTGCCCAC TTACATCCCT 
GACCGTGAGA TCACACCGCT GAAGAATCTT GAGAAGTGGG CACAGCTGGC CATTGCTGCC 
CACAAGAAGG GAATTTATGC CCAGAGGAGA ACTGACTCCC AGAAGGTCAA AGAGGATGTG 
GTCAATTATG CCCGTTTCAA GTGGCCCTTG CTCTTCTCCA GGTTTTACGA AGCTTACAAA 
TTCTCAGGCC CTCCCCTCCC CAAGAGCGAC GTCATCGTGG CTGTCAACTG GACGGGTGTG 
TACTTCGTGG ACGAGCAGGA GCAGGTGCTT CTGGAGCTGT CCTTCCCGGA GATCATGGCT 
GTGTCCAGCA GTAGGGAGTG CCGCGTCTTG CTCTCACTGG GCTGCTCTGA CTTGGGCTGT 
GCTACTTGTC AATCGGGCCG GGCAGGGCTG ACCCCGGCTG GACCCTGTTC TCCGTGTTGG 
TCCTGTAGGG GAACAAAGAT GATGGCCCCC AGCTTTACCC TGGCCACCAT CAAAGGAGAT 
GAGTACACCT TCACATCCAG CAATGCTGAG GACATCCGTG ACCTGGTGGT CACCTTTCTG 
GAGGGGCTAC GGAAGAGGTC TAAGTATGTG GTGGCACTGC AGGACAATCC TAACCCTGCT 
GGTGAGGAGT CAGGCTTCCT CAGCTTCGCC AAGGGAGACC TCATCATCCT TGACCATGAT 
ACTGGTGAGC AGGTCATGAA CTCAGGCTGG GCCAACGGCA TCAACGAGAG GACCAAGCAG 
CGCGGCGACT TCCCCACTGA CTGTGTATAC GTCATGCCCA CTGTCACCTT GCCACCAAGG 
GAGATTGTGG CCCTGGTCAC TATGACCCCA GACCAGAGGC AGGATGTCGT CCGGCTCCTG 
CAGCTTCGCA CAGCAGAGCC AGAGGTGCGC GCCAAGCCCT ACACGCTAGA GGAGTTCTCC 
TACGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGCGGTAAGG ACAGGCTGTG GAGCCACACA CGAGAGCCCC TCAAGCAGGC CCTGCTCAAG 
AAGATCCTGG GCAGTGAAGA ACTCTCCCAG GAAGCCTGCA TGGCCTTTGT AGCTGTGCTC 
AAGTACATGG GCGACTACCC ATCCAAGAGG ATGCGATCCG TCAATGAGCT CACTGACCAG 
ATCTTTGAGT GGGCACTCAA GGCTGAGCCC CTCAAGGATG AGGCCTACGT GCAGATCCTG 
AAGCAGCTGA CTGACAATCA CATCAGGTAC AGCGAAGAGA GGGGCTGGGA ACTGCTGTGG 
CTGTGCACGG GCCTCTTCCC GCCCAGCAAC ATCCTCCTGC CTCATGTTCA GCGGTTTCTG 
CAGTCCCGCA AGCACTGTCC TCTTGCCATT GACTGCCTGC AGAGGCTCCA GAAAGCCCTG 
AGAAATGGCT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAACATAAG 
ACTACCCAGA TCTTCCACAA GGTCTACTTC CCCGATGACA CGGACGAGGC TTTTGAGGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCAGCCGGCT GCTGCTCAAG 
TCTTCCGAGG GATTCAGCCT TTTTGTCAAA ATCGCAGATA AGGTCATCAG CGTCCCAGAG 
AATGATTTCT TCTTTGACTT TGTCCGACAC CTGACAGACT GGATAAAGAA AGCACGGCCC 
ATCAAGGACG GAATCGTGCC CTCACTAACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACAG TGCCGGGCAA GGACCCCATG GCTGACTCCA TCTTCCACTA TTACCAGGAA 
CTGCCCAAAT ATCTCCGAGG CTACCACAAG TGCACCCGGG AGGAGGTGCT GCAGCTGGGC 
GCACTCATCT ACAGGGTCAA GTTTGAGGAG GACAAATCCT ACTTCCCTAG CATCCCCAAG 
TTGCTGAGGG AGCTGGTACC CCAGGACCTA ATCCGGCAGG TCTCACCTGA TGACTGGAAA 
CGGTCTATTG TCGCCTACTT CAACAAACAT GCGGGGAAGT CCAAGGAGGA AGCCAAGCTG 
GCCTTCCTCA AACTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT TGAGGTGAAG 
CAAACTACAG AACCAAACTT CCCAGAGATT CTCTTAATTG CCATCAACAA GTACGGGGTC 
AGCCTCATCG ATCCCAGAAC CAAGGACATC CTGACTACTC ACCCCTTCAC CAAGATCTCC 
AACTGGAGTA GTGGCAACAC CTACTTCCAC ATCACCATTG GGAACTTGGT CCGTGGGAGC 
AAACTGCTCT GTGAGACATC GCTGGGATAC AAAATGGATG ATCTTCTGAC TTCCTACATC 
AGCCAGATGC TCACAGCCAT GAGCAAGCAG AGGAACTCCA GGAGTGGAAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo7a ( XM_011241688.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239990.1 Mus musculus myosin VIIA (Myo7a), transcript variant X1, mRNA.

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CloneID	OMu68262
Clone ID Related Accession (Same CDS sequence)	XM_011241688.2 , XM_011241688.3
Accession Version	XM_011241688.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-VIIa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGAC AGTGGCATCT GGCTCAGGGC TCTGCATCGA CTGGAGCCTG GTACCTCATC 
TTGTTTAGAT ACCGGGACAC AGGAATGCTG CCAAGGTCCT ACAAGGGGGA CTATGTATGG 
ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGAGTGC 
CGCGTCTTGC TCTCACTGGG CTGCTCTGAC TTGGGCTGTG CTACTTGTCA ATCGGGCCGG 
GCAGGGCTGA CCCCGGCTGG ACCCTGTTCT CCGTGTTGGT CCTGTAGGGG AACAAAGATG 
ATGGCCCCCA GCTTTACCCT GGCCACCATC AAAGGAGATG AGTACACCTT CACATCCAGC 
AATGCTGAGG ACATCCGTGA CCTGGTGGTC ACCTTTCTGG AGGGGCTACG GAAGAGGTCT 
AAGTATGTGG TGGCACTGCA GGACAATCCT AACCCTGCTG GTGAGGAGTC AGGCTTCCTC 
AGCTTCGCCA AGGGAGACCT CATCATCCTT GACCATGATA CTGGTGAGCA GGTCATGAAC 
TCAGGCTGGG CCAACGGCAT CAACGAGAGG ACCAAGCAGC GCGGCGACTT CCCCACTGAC 
TGTGTATACG TCATGCCCAC TGTCACCTTG CCACCAAGGG AGATTGTGGC CCTGGTCACT 
ATGACCCCAG ACCAGAGGCA GGATGTCGTC CGGCTCCTGC AGCTTCGCAC AGCAGAGCCA 
GAGGTGCGCG CCAAGCCCTA CACGCTAGAG GAGTTCTCCT ACGACTACTT CAGGCCCCCA 
CCCAAGCACA CGCTGAGCCG TGTCATGGTG TCCAAGGCCC GCGGTAAGGA CAGGCTGTGG 
AGCCACACAC GAGAGCCCCT CAAGCAGGCC CTGCTCAAGA AGATCCTGGG CAGTGAAGAA 
CTCTCCCAGG AAGCCTGCAT GGCCTTTGTA GCTGTGCTCA AGTACATGGG CGACTACCCA 
TCCAAGAGGA TGCGATCCGT CAATGAGCTC ACTGACCAGA TCTTTGAGTG GGCACTCAAG 
GCTGAGCCCC TCAAGGATGA GGCCTACGTG CAGATCCTGA AGCAGCTGAC TGACAATCAC 
ATCAGGTACA GCGAAGAGAG GGGCTGGGAA CTGCTGTGGC TGTGCACGGG CCTCTTCCCG 
CCCAGCAACA TCCTCCTGCC TCATGTTCAG CGGTTTCTGC AGTCCCGCAA GCACTGTCCT 
CTTGCCATTG ACTGCCTGCA GAGGCTCCAG AAAGCCCTGA GAAATGGCTC CCGGAAGTAC 
CCTCCGCACC TGGTGGAGGT GGAGGCCATC CAACATAAGA CTACCCAGAT CTTCCACAAG 
GTCTACTTCC CCGATGACAC GGACGAGGCT TTTGAGGTGG AGTCCAGCAC CAAGGCCAAG 
GACTTCTGCC AGAACATCGC CAGCCGGCTG CTGCTCAAGT CTTCCGAGGG ATTCAGCCTT 
TTTGTCAAAA TCGCAGATAA GGTCATCAGC GTCCCAGAGA ATGATTTCTT CTTTGACTTT 
GTCCGACACC TGACAGACTG GATAAAGAAA GCACGGCCCA TCAAGGACGG AATCGTGCCC 
TCACTAACCT ACCAGGTGTT CTTCATGAAG AAGCTGTGGA CCACCACAGT GCCGGGCAAG 
GACCCCATGG CTGACTCCAT CTTCCACTAT TACCAGGAAC TGCCCAAATA TCTCCGAGGC 
TACCACAAGT GCACCCGGGA GGAGGTGCTG CAGCTGGGCG CACTCATCTA CAGGGTCAAG 
TTTGAGGAGG ACAAATCCTA CTTCCCTAGC ATCCCCAAGT TGCTGAGGGA GCTGGTACCC 
CAGGACCTAA TCCGGCAGGT CTCACCTGAT GACTGGAAAC GGTCTATTGT CGCCTACTTC 
AACAAACATG CGGGGAAGTC CAAGGAGGAA GCCAAGCTGG CCTTCCTCAA ACTCATCTTC 
AAGTGGCCCA CCTTTGGCTC AGCCTTCTTT GAGGTGAAGC AAACTACAGA ACCAAACTTC 
CCAGAGATTC TCTTAATTGC CATCAACAAG TACGGGGTCA GCCTCATCGA TCCCAGAACC 
AAGGACATCC TGACTACTCA CCCCTTCACC AAGATCTCCA ACTGGAGTAG TGGCAACACC 
TACTTCCACA TCACCATTGG GAACTTGGTC CGTGGGAGCA AACTGCTCTG TGAGACATCG 
CTGGGATACA AAATGGATGA TCTTCTGACT TCCTACATCA GCCAGATGCT CACAGCCATG 
AGCAAGCAGA GGAACTCCAG GAGTGGAAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239990.1
CDS	23..6775
Translation	MDRQWHLAQGSASTGAWYLILFRYRDTGMLPRSYKGDYVWMDLKSGQEFDVPIGAVVKLCDSGQIQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTSCGGRTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNNRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHVFYCMLEGMNEEEKKKLGLGQAADYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHMGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPPLEVKNSRRSIGLLDIFGFENFTVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQEALDMIANRPMNVISLIDEESKFPKGTDATMLHKLNSQHKLNANYVPPKNSHETQFGINHFAGVVYYESQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRHAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLRLKSAATLIQRHWRGHHCRKNYELIRLGFLRLQALHRSRKLHKQYRLARQRIIEFQARCRAYLVRKAFRHRLWAVITVQAYARGMIARRLHRRLRVEYQRRLEAERMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEEARRKKELLEQMEKARHEPINHSDMVDKMFGFLGTSGSLPGQEGQAPSGFEDLERGRREMVEEDVDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGETQLPEGQKKTSVRHKLVHLTLKKKSKLTEEVTKRLNDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTARELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHNPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLSLVPTYIPDREITPLKNLEKWAQLAIAAHKKGIYAQRRTDSQKVKEDVVNYARFKWPLLFSRFYEAYKFSGPPLPKSDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVLLSLGCSDLGCATCQSGRAGLTPAGPCSPCWSCRGTKMMAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDCVYVMPTVTLPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKILGSEELSQEACMAFVAVLKYMGDYPSKRMRSVNELTDQIFEWALKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIASRLLLKSSEGFSLFVKIADKVISVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPRTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRNSRSGR

Target ORF information:

RefSeq Version	XM_011241688.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin VIIA (Myo7a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241688.2

ORF Insert Sequence:

ATGGATCGAC AGTGGCATCT GGCTCAGGGC TCTGCATCGA CTGGAGCCTG GTACCTCATC 
TTGTTTAGAT ACCGGGACAC AGGAATGCTG CCAAGGTCCT ACAAGGGGGA CTATGTATGG 
ATGGACCTGA AGTCAGGCCA GGAGTTTGAT GTGCCCATCG GGGCCGTGGT GAAGCTCTGC 
GACTCGGGCC AGATCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT ATCCCCTCAG 
AATGCCACGC ACATCAAGCC AATGCACCCC ACATCGGTGC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ATCTCAACGA GGCAGGCATC CTTCGAAACC TTCTCATTCG CTACCGGGAC 
CACCTCATCT ATACCAGCTG TGGAGGACGG ACGTACACAG GTTCCATCCT GGTGGCCGTG 
AACCCCTACC AGCTGCTCTC CATCTACTCG CCAGAGCACA TCCGCCAGTA CACCAACAAG 
AAGATAGGGG AGATGCCCCC CCACATCTTC GCCATTGCTG ACAACTGCTA CTTCAACATG 
AAACGCAACA ACCGGGACCA GTGCTGTATT ATCAGCGGGG AGTCGGGAGC TGGCAAGACA 
GAGAGCACAA AGTTGATCCT GCAGTTCCTG GCAGCCATCA GTGGACAGCA CTCATGGATC 
GAGCAGCAGG TGCTGGAGGC CACCCCGATC CTGGAAGCAT TTGGGAACGC CAAGACCATC 
CGCAACGACA ACTCTAGCCG CTTTGGCAAG TACATTGACA TCCACTTTAA CAAGCGTGGT 
GCCATCGAGG GCGCCAAAAT AGAGCAATAC CTGCTGGAGA AGTCACGTGT CTGCCGCCAG 
GCCCCTGACG AGAGGAACTA TCACGTGTTC TACTGTATGC TGGAGGGCAT GAATGAGGAG 
GAGAAGAAGA AACTGGGCCT AGGCCAGGCC GCTGACTACA ACTACTTGGC CATGGGTAAC 
TGCATCACCT GTGAGGGCCG CGTGGACAGT CAGGAGTATG CCAACATCCG CTCTGCCATG 
AAGGTTCTCA TGTTCACAGA CACGGAGAAC TGGGAGATCT CGAAGCTTCT GGCTGCCATC 
CTACACATGG GCAATCTGCA GTATGAGGCC CGGACATTTG AGAACTTGGA TGCGTGTGAA 
GTCCTCTTCT CCCCATCGCT GGCCACGGCA GCTTCTCTGC TCGAGGTGAA CCCCCCAGAC 
CTGATGAGCT GCCTCACCAG CCGCACCCTC ATCACCCGTG GGGAGACGGT GTCCACCCCT 
CTCAGCAGGG AACAGGCGCT GGATGTGCGA GATGCCTTTG TCAAGGGCAT CTATGGGCGG 
CTCTTTGTGT GGATTGTGGA GAAGATCAAC GCAGCAATCT ACAAGCCACC CCCCCTGGAA 
GTGAAGAACT CTCGCCGGTC CATCGGTCTC CTGGACATCT TTGGATTTGA GAACTTCACT 
GTGAACAGCT TCGAGCAGCT CTGCATTAAC TTTGCCAATG AGCACCTGCA GCAATTCTTC 
GTGCGGCACG TGTTCAAGCT GGAGCAGGAG GAGTACGACC TGGAGAGCAT CGACTGGTTG 
CACATTGAGT TCACTGACAA CCAGGAAGCA CTGGACATGA TTGCCAACCG GCCTATGAAC 
GTCATCTCCC TCATCGATGA GGAGAGCAAG TTCCCCAAGG GCACGGATGC CACCATGCTG 
CATAAGCTGA ACTCACAGCA CAAGCTCAAT GCCAACTACG TGCCACCCAA GAACAGCCAC 
GAGACCCAGT TTGGAATCAA CCACTTTGCG GGTGTTGTCT ATTATGAGAG TCAAGGCTTC 
CTGGAGAAGA ACCGAGACAC CCTGCATGGG GACATCATCC AGCTGGTCCA CTCTTCCCGG 
AACAAGTTCA TAAAGCAGAT TTTCCAAGCT GACGTTGCCA TGGGTGCCGA GACCAGGAAG 
CGCTCGCCTA CACTCAGCAG CCAGTTCAAG CGGTCTCTGG AGCTGCTGAT GCGCACACTG 
GGCGCCTGCC AGCCCTTCTT TGTGCGTTGT ATCAAACCCA ATGAGTTCAA GAAGCCCATG 
CTCTTCGACC GGCACTTGTG TGTACGCCAG CTGCGATATT CGGGCATGAT GGAGACAATC 
CGCATCCGCC ACGCAGGCTA CCCCATTCGC TACAGCTTTG TGGAGTTTGT GGAGCGCTAC 
CGGGTACTGC TGCCTGGTGT GAAGCCAGCA TACAAGCAGG GTGACCTCCG AGGGACATGC 
CAGCGCATGG CTGAGGCTGT GCTGGGCACG CACGATGACT GGCAGATTGG CAAAACCAAG 
ATCTTTCTGA AGGACCACCA TGACATGTTG CTGGAGGTGG AGCGGGACAA GGCCATCACA 
GACAGAGTCA TTCTCCTCCA GAAGGTTATC CGGGGCTTCA AAGACAGGTC CAACTTCCTG 
AGACTGAAGA GTGCTGCCAC ACTGATCCAG AGGCACTGGC GGGGCCACCA CTGTAGGAAA 
AACTATGAGC TGATTCGTCT TGGCTTCCTG CGGCTGCAGG CCCTGCACCG CTCCCGGAAG 
CTGCACAAGC AGTACCGCCT GGCCAGACAG CGCATAATAG AGTTCCAGGC CCGCTGCCGG 
GCCTATCTGG TGCGCAAGGC CTTCCGCCAC CGCCTCTGGG CCGTGATCAC CGTGCAGGCC 
TATGCCCGAG GCATGATTGC CCGCCGGCTA CACCGCCGCC TCCGGGTTGA GTACCAGCGG 
CGCCTCGAGG CAGAGAGGAT GCGTCTGGCA GAGGAGGAGA AACTCCGAAA GGAGATGAGT 
GCCAAGAAGG CCAAAGAGGA GGCTGAGCGC AAGCATCAGG AGCGCCTGGC TCAGCTAGCC 
CGCGAGGATG CGGAGCGGGA ACTGAAGGAG AAGGAGGAGG CTCGGAGGAA GAAGGAACTG 
CTGGAGCAGA TGGAGAAGGC CCGCCACGAA CCCATCAACC ACTCAGATAT GGTGGACAAG 
ATGTTTGGCT TCCTGGGGAC TTCAGGCAGC CTGCCAGGCC AGGAAGGCCA GGCGCCTAGT 
GGCTTTGAGG ACCTAGAGCG CGGACGGAGG GAGATGGTGG AAGAGGATGT TGACGCTGCC 
CTGCCCCTGC CTGATGAAGA CGAGGAGGAC CTTTCTGAGT ACAAATTCGC CAAGTTTGCT 
GCCACCTACT TCCAGGGCAC AACCACACAC TCCTACACCC GGAGGCCTCT CAAGCAGCCG 
CTGCTCTACC ACGACGATGA GGGTGACCAG CTGGCGGCGC TGGCTGTCTG GATCACCATC 
CTCCGGTTCA TGGGGGACCT CCCAGAGCCC AAGTACCACA CAGCCATGAG CGACGGCAGT 
GAGAAGATCC CAGTGATGAC TAAGATCTAC GAGACCCTAG GCAAGAAGAC ATATAAGAGG 
GAGCTGCAGG CCTTGCAGGG CGAGGGCGAG ACCCAGCTCC CTGAGGGGCA GAAGAAGACC 
AGTGTGAGAC ACAAGTTGGT ACACTTGACA CTGAAGAAAA AGTCCAAACT CACAGAAGAG 
GTGACCAAGA GGCTGAACGA TGGGGAATCC ACGGTACAGG GCAACAGCAT GCTGGAGGAT 
CGGCCCACCT CAAATCTAGA GAAGCTGCAC TTCATCATCG GCAACGGCAT CCTGCGGCCT 
GCGCTGCGGG ACGAGATTTA CTGCCAGATC AGTAAGCAGC TCACACACAA CCCATCCAAG 
AGCAGCTATG CCAGGGGCTG GATCCTCGTG TCGCTCTGTG TGGGCTGCTT CGCCCCCTCT 
GAGAAGTTCG TTAAGTACCT GCGGAACTTC ATCCACGGAG GCCCACCTGG CTATGCTCCT 
TACTGTGAGG AGCGCCTGAG GAGGACCTTT GTCAACGGAA CTCGGACACA GCCACCCAGC 
TGGCTGGAGC TGCAGGCCAC CAAGTCCAAG AAGCCCATCA TGTTGCCCGT GACCTTCATG 
GATGGGACCA CCAAGACCCT GCTAACAGAT TCAGCAACTA CAGCCAGGGA GCTGTGCAAT 
GCTCTGGCTG ACAAGATCTC ACTCAAGGAC CGCTTTGGCT TCTCCCTCTA CATCGCTCTG 
TTCGATAAGG TGTCCTCCCT GGGCAGCGGC AGTGACCATG TCATGGATGC CATCTCTCAG 
TGTGAGCAGT ACGCCAAGGA GCAGGGTGCT CAGGAGCGCA ACGCCCCATG GAGGCTCTTC 
TTTAGAAAGG AGGTCTTCAC ACCCTGGCAC AACCCCTCGG AGGACAACGT GGCCACGAAC 
CTCATCTACC AGCAGGTGGT GCGAGGAGTC AAGTTTGGGG AGTACAGGTG TGAGAAGGAG 
GACGACCTGG CTGAGCTGGC TTCTCAGCAG TACTTTGTGG ACTATGGTTC TGAGATGATT 
CTGGAGCGCC TGCTGAGCCT CGTGCCCACT TACATCCCTG ACCGTGAGAT CACACCGCTG 
AAGAATCTTG AGAAGTGGGC ACAGCTGGCC ATTGCTGCCC ACAAGAAGGG AATTTATGCC 
CAGAGGAGAA CTGACTCCCA GAAGGTCAAA GAGGATGTGG TCAATTATGC CCGTTTCAAG 
TGGCCCTTGC TCTTCTCCAG GTTTTACGAA GCTTACAAAT TCTCAGGCCC TCCCCTCCCC 
AAGAGCGACG TCATCGTGGC TGTCAACTGG ACGGGTGTGT ACTTCGTGGA CGAGCAGGAG 
CAGGTGCTTC TGGAGCTGTC CTTCCCGGAG ATCATGGCTG TGTCCAGCAG TAGGGAGTGC 
CGCGTCTTGC TCTCACTGGG CTGCTCTGAC TTGGGCTGTG CTACTTGTCA ATCGGGCCGG 
GCAGGGCTGA CCCCGGCTGG ACCCTGTTCT CCGTGTTGGT CCTGTAGGGG AACAAAGATG 
ATGGCCCCCA GCTTTACCCT GGCCACCATC AAAGGAGATG AGTACACCTT CACATCCAGC 
AATGCTGAGG ACATCCGTGA CCTGGTGGTC ACCTTTCTGG AGGGGCTACG GAAGAGGTCT 
AAGTATGTGG TGGCACTGCA GGACAATCCT AACCCTGCTG GTGAGGAGTC AGGCTTCCTC 
AGCTTCGCCA AGGGAGACCT CATCATCCTT GACCATGATA CTGGTGAGCA GGTCATGAAC 
TCAGGCTGGG CCAACGGCAT CAACGAGAGG ACCAAGCAGC GCGGCGACTT CCCCACTGAC 
TGTGTATACG TCATGCCCAC TGTCACCTTG CCACCAAGGG AGATTGTGGC CCTGGTCACT 
ATGACCCCAG ACCAGAGGCA GGATGTCGTC CGGCTCCTGC AGCTTCGCAC AGCAGAGCCA 
GAGGTGCGCG CCAAGCCCTA CACGCTAGAG GAGTTCTCCT ACGACTACTT CAGGCCCCCA 
CCCAAGCACA CGCTGAGCCG TGTCATGGTG TCCAAGGCCC GCGGTAAGGA CAGGCTGTGG 
AGCCACACAC GAGAGCCCCT CAAGCAGGCC CTGCTCAAGA AGATCCTGGG CAGTGAAGAA 
CTCTCCCAGG AAGCCTGCAT GGCCTTTGTA GCTGTGCTCA AGTACATGGG CGACTACCCA 
TCCAAGAGGA TGCGATCCGT CAATGAGCTC ACTGACCAGA TCTTTGAGTG GGCACTCAAG 
GCTGAGCCCC TCAAGGATGA GGCCTACGTG CAGATCCTGA AGCAGCTGAC TGACAATCAC 
ATCAGGTACA GCGAAGAGAG GGGCTGGGAA CTGCTGTGGC TGTGCACGGG CCTCTTCCCG 
CCCAGCAACA TCCTCCTGCC TCATGTTCAG CGGTTTCTGC AGTCCCGCAA GCACTGTCCT 
CTTGCCATTG ACTGCCTGCA GAGGCTCCAG AAAGCCCTGA GAAATGGCTC CCGGAAGTAC 
CCTCCGCACC TGGTGGAGGT GGAGGCCATC CAACATAAGA CTACCCAGAT CTTCCACAAG 
GTCTACTTCC CCGATGACAC GGACGAGGCT TTTGAGGTGG AGTCCAGCAC CAAGGCCAAG 
GACTTCTGCC AGAACATCGC CAGCCGGCTG CTGCTCAAGT CTTCCGAGGG ATTCAGCCTT 
TTTGTCAAAA TCGCAGATAA GGTCATCAGC GTCCCAGAGA ATGATTTCTT CTTTGACTTT 
GTCCGACACC TGACAGACTG GATAAAGAAA GCACGGCCCA TCAAGGACGG AATCGTGCCC 
TCACTAACCT ACCAGGTGTT CTTCATGAAG AAGCTGTGGA CCACCACAGT GCCGGGCAAG 
GACCCCATGG CTGACTCCAT CTTCCACTAT TACCAGGAAC TGCCCAAATA TCTCCGAGGC 
TACCACAAGT GCACCCGGGA GGAGGTGCTG CAGCTGGGCG CACTCATCTA CAGGGTCAAG 
TTTGAGGAGG ACAAATCCTA CTTCCCTAGC ATCCCCAAGT TGCTGAGGGA GCTGGTACCC 
CAGGACCTAA TCCGGCAGGT CTCACCTGAT GACTGGAAAC GGTCTATTGT CGCCTACTTC 
AACAAACATG CGGGGAAGTC CAAGGAGGAA GCCAAGCTGG CCTTCCTCAA ACTCATCTTC 
AAGTGGCCCA CCTTTGGCTC AGCCTTCTTT GAGGTGAAGC AAACTACAGA ACCAAACTTC 
CCAGAGATTC TCTTAATTGC CATCAACAAG TACGGGGTCA GCCTCATCGA TCCCAGAACC 
AAGGACATCC TGACTACTCA CCCCTTCACC AAGATCTCCA ACTGGAGTAG TGGCAACACC 
TACTTCCACA TCACCATTGG GAACTTGGTC CGTGGGAGCA AACTGCTCTG TGAGACATCG 
CTGGGATACA AAATGGATGA TCTTCTGACT TCCTACATCA GCCAGATGCT CACAGCCATG 
AGCAAGCAGA GGAACTCCAG GAGTGGAAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Myo7a
Entrez Gene ID	17921
Full Name	myosin VIIA
Synonyms	Hdb,Myo7,USH1B,nmf371,polka,sh-1,sh1
General protein information	Preferred Names myosin VIIA Names unconventional myosin-VIIa motor protein myosin-VIIa shaker 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 E1\|7 53.57 cM

	MYO7A	XP_001087868.2
Gallus gallus (chicken)	MYO7A	XP_004938998.1
Danio rerio (zebrafish)	myo7aa	NP_694515.1
Rattus norvegicus (Norway rat)	Myo7a	NP_703203.1
Xenopus tropicalis (tropical clawed frog)	myo7a	XP_004912319.1
Homo sapiens (human)	MYO7A	NP_000251.3
Bos taurus (cattle)	MYO7A	XP_005216396.1
Pan troglodytes (chimpanzee)	MYO7A	XP_003313297.2
Canis lupus familiaris (dog)	MYO7A	XP_005633514.1
Mus musculus (house mouse)	Myo7a	NP_001243010.1
Drosophila melanogaster (fruit fly)	ck	NP_523571.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Myo7a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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