GCLM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLM
Entrez Gene ID	2730
Full Name	glutamate-cysteine ligase modifier subunit
Synonyms	GLCLR
General protein information	Preferred Names glutamate-cysteine ligase modifier subunit Names glutamate--cysteine ligase regulatory subunit GCS light chain GSC light chain gamma-ECS regulatory subunit gamma-glutamylcysteine synthetase regulatory subunit glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD) glutamate-cysteine ligase modifier subunit delta2 alternative splicing glutamate-cysteine ligase regulatory protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p22.1
Summary	Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015].
Disorder MIM:	601176
Disorder Html:	{Myocardial infarction, susceptibility to}, 608446 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002061.3	NP_002052.1	glutamate--cysteine ligase regulatory subunit isoform 1
XM_017001057.2	XP_016856546.1	glutamate--cysteine ligase regulatory subunit isoform X2
NM_001308253.1	NP_001295182.1	glutamate--cysteine ligase regulatory subunit isoform 2
NM_002061.4	NP_002052.1	glutamate--cysteine ligase regulatory subunit isoform 1
XM_017001056.2	XP_016856545.1	glutamate--cysteine ligase regulatory subunit isoform X1
XM_017001057.1	XP_016856546.1	glutamate--cysteine ligase regulatory subunit isoform X2
XM_011541261.2	XP_011539563.1	glutamate--cysteine ligase regulatory subunit isoform X3
XM_017001056.1	XP_016856545.1	glutamate--cysteine ligase regulatory subunit isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GCLM	NP_001033232.1
Gallus gallus (chicken)	GCLM	NP_001007954.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012038	XP_320488.3
Caenorhabditis elegans (roundworm)	E01A2.1	NP_001122439.1
Pan troglodytes (chimpanzee)	GCLM	XP_513572.3
Danio rerio (zebrafish)	gclm	NP_956139.1
Homo sapiens (human)	GCLM	NP_002052.1
Canis lupus familiaris (dog)	GCLM	XP_005621954.1
Xenopus tropicalis (tropical clawed frog)	gclm	NP_001016536.1
Mus musculus (house mouse)	Gclm	NP_032155.1
Rattus norvegicus (Norway rat)	Gclm	NP_059001.1
Drosophila melanogaster (fruit fly)	Gclm	NP_001262845.1

Gene Ontology
Bibliography

Related articles in PubMed

Temporal changes in glutathione biosynthesis during the lipopolysaccharide-induced inflammatory response of THP-1 macrophages.
Zhang H, Liu H, Zhou L, Yuen J, Forman HJ
Free radical biology & medicine113304-310(2017 Dec)

Association of GCLM -588C/T and GCLC -129T/C Promoter Polymorphisms of Genes Coding the Subunits of Glutamate Cysteine Ligase with Ischemic Heart Disease Development in Kazakhstan Population.
Skvortsova L, Perfelyeva A, Khussainova E, Mansharipova A, Forman HJ, Djansugurova L
Disease markers20174209257(2017)

HNF1? drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC).
Lopes-Coelho F, Gouveia-Fernandes S, Gon?alves LG, Nunes C, Faustino I, Silva F, F?lix A, Pereira SA, Serpa J
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(4)4813-29(2016 Apr)

Nrf2 is the key to chemotherapy resistance in MCF7 breast cancer cells under hypoxia.
Syu JP, Chi JT, Kung HN
Oncotarget7(12)14659-72(2016 Mar)

Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2-related factor 2-antioxidant response element signaling pathway in human endothelial cells.
Hiramatsu K, Tsuneyoshi T, Ogawa T, Morihara N
Nutrition research (New York, N.Y.)36(2)143-9(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1beta regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2
Title: HNF1? drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC).

Data suggest gene expression in vascular endothelium is altered by dietary factors; aged garlic extract induces expression of HMOX1 (heme oxygenase-1) and GCLM via activation of NRF2-ARE (nuclear factor erythroid 2-antioxidant response element) signaling.
Title: Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2-related factor 2-antioxidant response element signaling pathway in human endothelial cells.

This study demonstrated that Tuberous sclerosis complex neuropathology requires glutamate-cysteine ligase.
Title: Tuberous sclerosis complex neuropathology requires glutamate-cysteine ligase.

Knockdown of glutamate cysteine ligase catalytic subunit by siRNA causes the gold nanoparticles-induced cytotoxicity in lung cancer cells.
Title: Knockdown of glutamate cysteine ligase catalytic subunit by siRNA causes the gold nanoparticles-induced cytotoxicity in lung cancer cells.

Data suggest expression of hepatocyte GCLC and GCLM can be regulated by dietary component; alpha-lipoic acid, a vitamin B complex nutrient, protects against oxidative stress/cytotoxicity induced by cadmium via restoration of GCLC and GCLM expression.
Title: ?-Lipoic acid protects against the oxidative stress and cytotoxicity induced by cadmium in HepG2 cells through regenerating glutathione regulated by glutamate-cysteine ligase.

The following GCLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18973	NM_002061.4 Latest version!	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu57978	XM_011541261.2 Latest version!	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu33351	NM_001308253.1 Latest version!	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu87261	XM_017001056.2 Latest version!	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu87262	XM_017001057.2 Latest version!	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu18973	NM_002061.3	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu87262	XM_017001057.1	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu87261	XM_017001056.1	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLM ( NM_002061.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002052.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1, mRNA.

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CloneID	OHu18973
Clone ID Related Accession (Same CDS sequence)	NM_002061.3 , NM_002061.4
Accession Version	NM_002061.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290449.1, BG501583.1, BC041809.1, CB123306.1, CN272168.1, BQ049850.1, N53134.1, CN422503.1, BF513627.1, BM982562.1 and BU754400.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2015 this sequence version replaced NM_002061.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290449.1, SRR1163657.567749.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAACTG 
CTTTCTGAAG CAAGTTTCCA AGAAGCTCTT CAGGAAAGCA TTCCTGACAT TCAAGCGCAC 
GAGTGGGTGC CGCTGTGGCT ACTGCGGTAT TCGGTCATTG TGAAAAGTAG AGGAATTATC 
AAATCAAAAG GCTACATTTT ACAAGCTAAA AGAAGGGGTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002052.1
CDS	436..1260
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Target ORF information:

RefSeq Version	NM_002061.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002061.3

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAACTG 
CTTTCTGAAG CAAGTTTCCA AGAAGCTCTT CAGGAAAGCA TTCCTGACAT TCAAGCGCAC 
GAGTGGGTGC CGCTGTGGCT ACTGCGGTAT TCGGTCATTG TGAAAAGTAG AGGAATTATC 
AAATCAAAAG GCTACATTTT ACAAGCTAAA AGAAGGGGTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( NM_002061.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002052.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18973
Clone ID Related Accession (Same CDS sequence)	NM_002061.3 , NM_002061.4
Accession Version	NM_002061.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290449.1, BC041809.1, CB123306.1, CN272168.1, BQ049850.1, N53134.1, CN422503.1, BF513627.1, BM982562.1 and BU754400.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002061.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.644211.1, SRR1163655.300794.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370238.8/ ENSP00000359258.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAACTG 
CTTTCTGAAG CAAGTTTCCA AGAAGCTCTT CAGGAAAGCA TTCCTGACAT TCAAGCGCAC 
GAGTGGGTGC CGCTGTGGCT ACTGCGGTAT TCGGTCATTG TGAAAAGTAG AGGAATTATC 
AAATCAAAAG GCTACATTTT ACAAGCTAAA AGAAGGGGTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002052.1
CDS	268..1092
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Target ORF information:

RefSeq Version	NM_002061.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002061.4

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAACTG 
CTTTCTGAAG CAAGTTTCCA AGAAGCTCTT CAGGAAAGCA TTCCTGACAT TCAAGCGCAC 
GAGTGGGTGC CGCTGTGGCT ACTGCGGTAT TCGGTCATTG TGAAAAGTAG AGGAATTATC 
AAATCAAAAG GCTACATTTT ACAAGCTAAA AGAAGGGGTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( XM_011541261.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539563.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu57978
Clone ID Related Accession (Same CDS sequence)	XM_011541261.2
Accession Version	XM_011541261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTT CTGCAAAACT GTTCATTGTA GAATCAAACT CTTCATCATC AACTAGAAGT 
GCAGTTGACA TGGCCTGTTC AGTCCTTGGA GTTGCACAGC TGGATTCTGT GATCATTGCT 
TCACCTCCTA TTGAAGATGG AGTTAATCTT TCCTTGGAGC ATTTACAGCC TTACTGGGAG 
GAATTAGAAA ACTTAGTTCA GAGCAAAAAG ATTGTTGCCA TAGGTACCTC TGATCTAGAC 
AAAACACAGT TGGAACAGCT GTATCAGTGG GCACAGGTAA AACCAAATAG TAACCAAGTT 
AATCTTGCCT CCTGCTGTGT GATGCCACCA GATTTGACTG CATTTGCTAA ACAATTTGAC 
ATACAGCTGT TGACTCACAA TGATCCAAAA GAACTGCTTT CTGAAGCAAG TTTCCAAGAA 
GCTCTTCAGG AAAGCATTCC TGACATTCAA GCGCACGAGT GGGTGCCGCT GTGGCTACTG 
CGGTATTCGG TCATTGTGAA AAGTAGAGGA ATTATCAAAT CAAAAGGCTA CATTTTACAA 
GCTAAAAGAA GGGGTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539563.1
CDS	501..1061
Translation	MKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Target ORF information:

RefSeq Version	XM_011541261.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541261.2

ORF Insert Sequence:

ATGAAAGTTT CTGCAAAACT GTTCATTGTA GAATCAAACT CTTCATCATC AACTAGAAGT 
GCAGTTGACA TGGCCTGTTC AGTCCTTGGA GTTGCACAGC TGGATTCTGT GATCATTGCT 
TCACCTCCTA TTGAAGATGG AGTTAATCTT TCCTTGGAGC ATTTACAGCC TTACTGGGAG 
GAATTAGAAA ACTTAGTTCA GAGCAAAAAG ATTGTTGCCA TAGGTACCTC TGATCTAGAC 
AAAACACAGT TGGAACAGCT GTATCAGTGG GCACAGGTAA AACCAAATAG TAACCAAGTT 
AATCTTGCCT CCTGCTGTGT GATGCCACCA GATTTGACTG CATTTGCTAA ACAATTTGAC 
ATACAGCTGT TGACTCACAA TGATCCAAAA GAACTGCTTT CTGAAGCAAG TTTCCAAGAA 
GCTCTTCAGG AAAGCATTCC TGACATTCAA GCGCACGAGT GGGTGCCGCT GTGGCTACTG 
CGGTATTCGG TCATTGTGAA AAGTAGAGGA ATTATCAAAT CAAAAGGCTA CATTTTACAA 
GCTAAAAGAA GGGGTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( NM_001308253.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295182.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu33351
Clone ID Related Accession (Same CDS sequence)	NM_001308253.1
Accession Version	NM_001308253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290449.1, BG501583.1, AB809606.1, BC041809.1, CB123306.1, CN272168.1, BQ049850.1, N53134.1, CN422503.1, BF513627.1, BM982562.1 and BU754400.1. On May 15, 2015 this sequence version replaced XM_005270754.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2 which has the same N- and C-termini, but is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.101152.1, AB809606.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGGAGT TTCCAGATGT CTTGGAATGC ACTGTATCTC ATGCAGTAGA AAAGATAAAT 
CCTGATGAAA GAGAAGAAAT GAAAGTTTCT GCAAAACTGT TCATTGTAGA ATCAAACTCT 
TCATCATCAA CTAGAAGTGC AGTTGACATG GCCTGTTCAG TCCTTGGAGT TGCACAGCTG 
GATTCTGTGA TCATTGCTTC ACCTCCTATT GAAGATGGAG TTAATCTTTC CTTGGAGCAT 
TTACAGCCTT ACTGGGAGGA ATTAGAAAAC TTAGTTCAGA GCAAAAAGAT TGTTGCCATA 
GGTACCTCTG ATCTAGACAA AACACAGTTG GAACAGCTGT ATCAGTGGGC ACAGGTAAAA 
CCAAATAGTA ACCAAGTTAA TCTTGCCTCC TGCTGTGTGA TGCCACCAGA TTTGACTGCA 
TTTGCTAAAC AATTTGACAT ACAGCTGTTG ACTCACAATG ATCCAAAAGA ACTGCTTTCT 
GAAGCAAGTT TCCAAGAAGC TCTTCAGGAA AGCATTCCTG ACATTCAAGC GCACGAGTGG 
GTGCCGCTGT GGCTACTGCG GTATTCGGTC ATTGTGAAAA GTAGAGGAAT TATCAAATCA 
AAAGGCTACA TTTTACAAGC TAAAAGAAGG GGTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295182.1
CDS	436..1194
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEEEFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

Target ORF information:

RefSeq Version	NM_001308253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308253.1

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGGAGT TTCCAGATGT CTTGGAATGC ACTGTATCTC ATGCAGTAGA AAAGATAAAT 
CCTGATGAAA GAGAAGAAAT GAAAGTTTCT GCAAAACTGT TCATTGTAGA ATCAAACTCT 
TCATCATCAA CTAGAAGTGC AGTTGACATG GCCTGTTCAG TCCTTGGAGT TGCACAGCTG 
GATTCTGTGA TCATTGCTTC ACCTCCTATT GAAGATGGAG TTAATCTTTC CTTGGAGCAT 
TTACAGCCTT ACTGGGAGGA ATTAGAAAAC TTAGTTCAGA GCAAAAAGAT TGTTGCCATA 
GGTACCTCTG ATCTAGACAA AACACAGTTG GAACAGCTGT ATCAGTGGGC ACAGGTAAAA 
CCAAATAGTA ACCAAGTTAA TCTTGCCTCC TGCTGTGTGA TGCCACCAGA TTTGACTGCA 
TTTGCTAAAC AATTTGACAT ACAGCTGTTG ACTCACAATG ATCCAAAAGA ACTGCTTTCT 
GAAGCAAGTT TCCAAGAAGC TCTTCAGGAA AGCATTCCTG ACATTCAAGC GCACGAGTGG 
GTGCCGCTGT GGCTACTGCG GTATTCGGTC ATTGTGAAAA GTAGAGGAAT TATCAAATCA 
AAAGGCTACA TTTTACAAGC TAAAAGAAGG GGTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( XM_017001056.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856545.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu87261
Clone ID Related Accession (Same CDS sequence)	XM_017001056.2 , XM_017001056.1
Accession Version	XM_017001056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAGACA 
GGGTTTCACC ATGTCACCCA GGCTGGGCTT GAATTTCTGG ACTCAAGCAA TTCCCCCACC 
TCGGCCTCCC AAGGTGCTGG GATTTTAGGC ATGAGCCACT GTGCCTGGAC AAAAGGCTAC 
TTTATTCAAG GAGATAAATA TCTGGAAAAA GTGGCACATG TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856545.1
CDS	1201..2028
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKETGFHHVTQAGLEFLDSSNSPTSASQGAGILGMSHCAWTKGYFIQGDKYLEKVAHVI

Target ORF information:

RefSeq Version	XM_017001056.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001056.2

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAGACA 
GGGTTTCACC ATGTCACCCA GGCTGGGCTT GAATTTCTGG ACTCAAGCAA TTCCCCCACC 
TCGGCCTCCC AAGGTGCTGG GATTTTAGGC ATGAGCCACT GTGCCTGGAC AAAAGGCTAC 
TTTATTCAAG GAGATAAATA TCTGGAAAAA GTGGCACATG TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( XM_017001056.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856545.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu87261
Clone ID Related Accession (Same CDS sequence)	XM_017001056.2 , XM_017001056.1
Accession Version	XM_017001056.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAGACA 
GGGTTTCACC ATGTCACCCA GGCTGGGCTT GAATTTCTGG ACTCAAGCAA TTCCCCCACC 
TCGGCCTCCC AAGGTGCTGG GATTTTAGGC ATGAGCCACT GTGCCTGGAC AAAAGGCTAC 
TTTATTCAAG GAGATAAATA TCTGGAAAAA GTGGCACATG TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856545.1
CDS	436..1263
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKETGFHHVTQAGLEFLDSSNSPTSASQGAGILGMSHCAWTKGYFIQGDKYLEKVAHVI

Target ORF information:

RefSeq Version	XM_017001056.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001056.1

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGCTTC ATGATTGTAT CCAAAAAACC TTGAATGAAT GGAGTTCCCA AATCAACCCA 
GATTTGGTCA GGGAGTTTCC AGATGTCTTG GAATGCACTG TATCTCATGC AGTAGAAAAG 
ATAAATCCTG ATGAAAGAGA AGAAATGAAA GTTTCTGCAA AACTGTTCAT TGTAGAATCA 
AACTCTTCAT CATCAACTAG AAGTGCAGTT GACATGGCCT GTTCAGTCCT TGGAGTTGCA 
CAGCTGGATT CTGTGATCAT TGCTTCACCT CCTATTGAAG ATGGAGTTAA TCTTTCCTTG 
GAGCATTTAC AGCCTTACTG GGAGGAATTA GAAAACTTAG TTCAGAGCAA AAAGATTGTT 
GCCATAGGTA CCTCTGATCT AGACAAAACA CAGTTGGAAC AGCTGTATCA GTGGGCACAG 
GTAAAACCAA ATAGTAACCA AGTTAATCTT GCCTCCTGCT GTGTGATGCC ACCAGATTTG 
ACTGCATTTG CTAAACAATT TGACATACAG CTGTTGACTC ACAATGATCC AAAAGAGACA 
GGGTTTCACC ATGTCACCCA GGCTGGGCTT GAATTTCTGG ACTCAAGCAA TTCCCCCACC 
TCGGCCTCCC AAGGTGCTGG GATTTTAGGC ATGAGCCACT GTGCCTGGAC AAAAGGCTAC 
TTTATTCAAG GAGATAAATA TCTGGAAAAA GTGGCACATG TAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( XM_017001057.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856546.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu87262
Clone ID Related Accession (Same CDS sequence)	XM_017001057.2 , XM_017001057.1
Accession Version	XM_017001057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGGAGT TTCCAGATGT CTTGGAATGC ACTGTATCTC ATGCAGTAGA AAAGATAAAT 
CCTGATGAAA GAGAAGAAAT GAAAGTTTCT GCAAAACTGT TCATTGTAGA ATCAAACTCT 
TCATCATCAA CTAGAAGTGC AGTTGACATG GCCTGTTCAG TCCTTGGAGT TGCACAGCTG 
GATTCTGTGA TCATTGCTTC ACCTCCTATT GAAGATGGAG TTAATCTTTC CTTGGAGCAT 
TTACAGCCTT ACTGGGAGGA ATTAGAAAAC TTAGTTCAGA GCAAAAAGAT TGTTGCCATA 
GGTACCTCTG ATCTAGACAA AACACAGTTG GAACAGCTGT ATCAGTGGGC ACAGGTAAAA 
CCAAATAGTA ACCAAGTTAA TCTTGCCTCC TGCTGTGTGA TGCCACCAGA TTTGACTGCA 
TTTGCTAAAC AATTTGACAT ACAGCTGTTG ACTCACAATG ATCCAAAAGA GACAGGGTTT 
CACCATGTCA CCCAGGCTGG GCTTGAATTT CTGGACTCAA GCAATTCCCC CACCTCGGCC 
TCCCAAGGTG CTGGGATTTT AGGCATGAGC CACTGTGCCT GGACAAAAGG CTACTTTATT 
CAAGGAGATA AATATCTGGA AAAAGTGGCA CATGTAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856546.1
CDS	1208..1969
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEEEFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKETGFHHVTQAGLEFLDSSNSPTSASQGAGILGMSHCAWTKGYFIQGDKYLEKVAHVI

Target ORF information:

RefSeq Version	XM_017001057.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001057.2

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGGAGT TTCCAGATGT CTTGGAATGC ACTGTATCTC ATGCAGTAGA AAAGATAAAT 
CCTGATGAAA GAGAAGAAAT GAAAGTTTCT GCAAAACTGT TCATTGTAGA ATCAAACTCT 
TCATCATCAA CTAGAAGTGC AGTTGACATG GCCTGTTCAG TCCTTGGAGT TGCACAGCTG 
GATTCTGTGA TCATTGCTTC ACCTCCTATT GAAGATGGAG TTAATCTTTC CTTGGAGCAT 
TTACAGCCTT ACTGGGAGGA ATTAGAAAAC TTAGTTCAGA GCAAAAAGAT TGTTGCCATA 
GGTACCTCTG ATCTAGACAA AACACAGTTG GAACAGCTGT ATCAGTGGGC ACAGGTAAAA 
CCAAATAGTA ACCAAGTTAA TCTTGCCTCC TGCTGTGTGA TGCCACCAGA TTTGACTGCA 
TTTGCTAAAC AATTTGACAT ACAGCTGTTG ACTCACAATG ATCCAAAAGA GACAGGGTTT 
CACCATGTCA CCCAGGCTGG GCTTGAATTT CTGGACTCAA GCAATTCCCC CACCTCGGCC 
TCCCAAGGTG CTGGGATTTT AGGCATGAGC CACTGTGCCT GGACAAAAGG CTACTTTATT 
CAAGGAGATA AATATCTGGA AAAAGTGGCA CATGTAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLM ( XM_017001057.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856546.1 Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu87262
Clone ID Related Accession (Same CDS sequence)	XM_017001057.2 , XM_017001057.1
Accession Version	XM_017001057.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase regulatory subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGGAGT TTCCAGATGT CTTGGAATGC ACTGTATCTC ATGCAGTAGA AAAGATAAAT 
CCTGATGAAA GAGAAGAAAT GAAAGTTTCT GCAAAACTGT TCATTGTAGA ATCAAACTCT 
TCATCATCAA CTAGAAGTGC AGTTGACATG GCCTGTTCAG TCCTTGGAGT TGCACAGCTG 
GATTCTGTGA TCATTGCTTC ACCTCCTATT GAAGATGGAG TTAATCTTTC CTTGGAGCAT 
TTACAGCCTT ACTGGGAGGA ATTAGAAAAC TTAGTTCAGA GCAAAAAGAT TGTTGCCATA 
GGTACCTCTG ATCTAGACAA AACACAGTTG GAACAGCTGT ATCAGTGGGC ACAGGTAAAA 
CCAAATAGTA ACCAAGTTAA TCTTGCCTCC TGCTGTGTGA TGCCACCAGA TTTGACTGCA 
TTTGCTAAAC AATTTGACAT ACAGCTGTTG ACTCACAATG ATCCAAAAGA GACAGGGTTT 
CACCATGTCA CCCAGGCTGG GCTTGAATTT CTGGACTCAA GCAATTCCCC CACCTCGGCC 
TCCCAAGGTG CTGGGATTTT AGGCATGAGC CACTGTGCCT GGACAAAAGG CTACTTTATT 
CAAGGAGATA AATATCTGGA AAAAGTGGCA CATGTAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856546.1
CDS	436..1197
Translation	MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEEEFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKETGFHHVTQAGLEFLDSSNSPTSASQGAGILGMSHCAWTKGYFIQGDKYLEKVAHVI

Target ORF information:

RefSeq Version	XM_017001057.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001057.1

ORF Insert Sequence:

ATGGGCACCG ACAGCCGCGC GGCCAAGGCG CTCCTGGCGC GGGCCCGCAC CCTGCACCTG 
CAGACGGGGA ACCTGCTGAA CTGGGGCCGC CTGCGGAAGA AGTGCCCGTC CACGCACAGC 
GAGGAGGAGT TTCCAGATGT CTTGGAATGC ACTGTATCTC ATGCAGTAGA AAAGATAAAT 
CCTGATGAAA GAGAAGAAAT GAAAGTTTCT GCAAAACTGT TCATTGTAGA ATCAAACTCT 
TCATCATCAA CTAGAAGTGC AGTTGACATG GCCTGTTCAG TCCTTGGAGT TGCACAGCTG 
GATTCTGTGA TCATTGCTTC ACCTCCTATT GAAGATGGAG TTAATCTTTC CTTGGAGCAT 
TTACAGCCTT ACTGGGAGGA ATTAGAAAAC TTAGTTCAGA GCAAAAAGAT TGTTGCCATA 
GGTACCTCTG ATCTAGACAA AACACAGTTG GAACAGCTGT ATCAGTGGGC ACAGGTAAAA 
CCAAATAGTA ACCAAGTTAA TCTTGCCTCC TGCTGTGTGA TGCCACCAGA TTTGACTGCA 
TTTGCTAAAC AATTTGACAT ACAGCTGTTG ACTCACAATG ATCCAAAAGA GACAGGGTTT 
CACCATGTCA CCCAGGCTGG GCTTGAATTT CTGGACTCAA GCAATTCCCC CACCTCGGCC 
TCCCAAGGTG CTGGGATTTT AGGCATGAGC CACTGTGCCT GGACAAAAGG CTACTTTATT 
CAAGGAGATA AATATCTGGA AAAAGTGGCA CATGTAATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporal changes in glutathione biosynthesis during the lipopolysaccharide-induced inflammatory response of THP-1 macrophages.
Free radical biology & medicine113304-310(2017 Dec)
Zhang H,Liu H,Zhou L,Yuen J,Forman HJ

Association of GCLM -588C/T and GCLC -129T/C Promoter Polymorphisms of Genes Coding the Subunits of Glutamate Cysteine Ligase with Ischemic Heart Disease Development in Kazakhstan Population.
Disease markers20174209257(2017)
Skvortsova L,Perfelyeva A,Khussainova E,Mansharipova A,Forman HJ,Djansugurova L

HNF1? drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC).
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(4)4813-29(2016 Apr)
Lopes-Coelho F,Gouveia-Fernandes S,Gon?alves LG,Nunes C,Faustino I,Silva F,F?lix A,Pereira SA,Serpa J

Nrf2 is the key to chemotherapy resistance in MCF7 breast cancer cells under hypoxia.
Oncotarget7(12)14659-72(2016 Mar)
Syu JP,Chi JT,Kung HN

Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2-related factor 2-antioxidant response element signaling pathway in human endothelial cells.
Nutrition research (New York, N.Y.)36(2)143-9(2016 Feb)
Hiramatsu K,Tsuneyoshi T,Ogawa T,Morihara N

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GCLM cDNA ORF clone, Homo sapiens(human)

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