TRPM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRPM1
Entrez Gene ID	4308
Full Name	transient receptor potential cation channel subfamily M member 1
Synonyms	CSNB1C,LTRPC1,MLSN1
General protein information	Preferred Names transient receptor potential cation channel subfamily M member 1 Names transient receptor potential cation channel subfamily M member 1 long transient receptor potential channel 1 melastatin-1 transient receptor potential melastatin family
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q13.3
Summary	This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011].
Disorder MIM:	603576
Disorder Html:	Night blindness, congenital stationary (complete), 1C, autosomal recessive, 613216 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002420.6	NP_002411.3	transient receptor potential cation channel subfamily M member 1 isoform 2
NM_002420.5	NP_002411.3	transient receptor potential cation channel subfamily M member 1 isoform 2
NM_001252020.1	NP_001238949.1	transient receptor potential cation channel subfamily M member 1 isoform 1
NM_001252030.2	NP_001238959.1	transient receptor potential cation channel subfamily M member 1 isoform 4
NM_001252024.2	NP_001238953.1	transient receptor potential cation channel subfamily M member 1 isoform 3
NM_001252024.1	NP_001238953.1	transient receptor potential cation channel subfamily M member 1 isoform 3
NM_001252030.1	NP_001238959.1	transient receptor potential cation channel subfamily M member 1 isoform 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPM1	NP_001238949.1
Pan troglodytes (chimpanzee)	TRPM1	XP_003318096.1
	TRPM1	XP_001116180.2
Xenopus tropicalis (tropical clawed frog)	trpm1	XP_002934962.2
Caenorhabditis elegans (roundworm)	gon-2	NP_001122507.1
Canis lupus familiaris (dog)	TRPM1	XP_005618325.1
Bos taurus (cattle)	TRPM1	XP_002696651.2
Rattus norvegicus (Norway rat)	Trpm1	NP_001032822.1
Mus musculus (house mouse)	Trpm1	NP_001034193.2
Gallus gallus (chicken)	TRPM1	XP_425066.3

Gene Ontology
Bibliography

Related articles in PubMed

Founder Ashkenazi Jewish mutations of large deletion in the inherited retinal dystrophy genes.
Chiang JP, Luo H, Duan J, Ekstein J, Hirsch Y
Ophthalmic genetics39(1)135-136(2018 Jan-Feb)

Joint Analysis of Nuclear and Mitochondrial Variants in Age-Related Macular Degeneration Identifies Novel Loci TRPM1 and ABHD2/RLBP1.
Persad PJ, Heid IM, Weeks DE, Baird PN, de Jong EK, Haines JL, Pericak-Vance MA, Scott WK,
Investigative ophthalmology & visual science58(10)4027-4038(2017 Aug)

Structural basis of dual Ca
Li M, Zhang WK, Benvin NM, Zhou X, Su D, Li H, Wang S, Michailidis IE, Tong L, Li X, Yang J
Nature structural & molecular biology24(3)205-213(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results show that joint tests of main effects and gene-gene interaction reveal associations at some novel loci that were missed when considering main effects alone.
Title: Joint Analysis of Nuclear and Mitochondrial Variants in Age-Related Macular Degeneration Identifies Novel Loci TRPM1 and ABHD2/RLBP1.

Loss of TRPM1 mRNA expression appears to be a crucial event in the progression of melanoma to a more malignant, metastatic phenotype.
Title: TRPM1 (melastatin) expression is an independent predictor of overall survival in clinical AJCC stage I and II melanoma patients.

This study reveals the structural underpinnings of TRPML1's regulation, assembly and pathogenesis.
Title: Structural basis of dual Ca

Affected family members harboured the homozygous 1-bp deletion c.2394delC in exon 18 of the TRPM1 gene, whereas their unaffected parents were heterozygous carriers.
Title: Congenital stationary night blindness with hypoplastic discs, negative electroretinogram and thinning of the inner nuclear layer.

these data indicate that purified TRPM1 is mostly dimeric. The three-dimensional structure of TRPM1 dimers is characterized by a small putative transmembrane domain and a larger domain with a hollow cavity.
Title: Oligomeric state of purified transient receptor potential melastatin-1 (TRPM1), a protein essential for dim light vision.

The following TRPM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26661	NM_001252020.1 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu14795	NM_001252030.2 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu14262	NM_001252024.2 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu20829	NM_002420.6 Latest version!	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu20829	NM_002420.5	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu14262	NM_001252024.1	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu14795	NM_001252030.1	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPM1 ( NM_001252020.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238949.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 1, mRNA.

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CloneID	OHu26661
Clone ID Related Accession (Same CDS sequence)	NM_001252020.1
Accession Version	NM_001252020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HM135791.1 and BU743036.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM135791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCCT TCAAGCGGGG CTCGCTCAAG AGCTCCACAT CAGGGTCGCA GAAGGGTCAG 
AAATCTTGGA TAGAGAAAAC CTTTTGCAAA CGGGAATGTA TCTTTGTAAT TCCTAGCATG 
AAAGACTCTA ACAGGTGTTG CTGTGGCCAG TTCACCAACC AGCATATCCC CCCTCTGCCA 
AGTGCAACAC CCAGCAAAAA TGAAGAGGAA AGCAAACAGG TGGAGACTCA GCCTGAGAAA 
TGGTCTGTTG CCAAGCACAC CCAGAGCTAC CCAACAGATT CCTATGGAGT TCTTGAATTC 
CAGGGTGGCG GATATTCCAA TAAAGCCATG TATATCCGTG TATCCTATGA CACCAAGCCA 
GACTCACTGC TCCATCTCAT GGTGAAAGAT TGGCAGCTGG AACTCCCCAA GCTCTTAATA 
TCTGTGCATG GAGGCCTCCA GAACTTTGAG ATGCAGCCCA AGCTGAAACA AGTCTTTGGG 
AAAGGCCTGA TCAAGGCTGC TATGACCACC GGGGCCTGGA TCTTCACCGG GGGTGTCAGC 
ACAGGTGTTA TCAGCCACGT AGGGGATGCC TTGAAAGACC ACTCCTCCAA GTCCAGAGGC 
CGGGTTTGTG CTATAGGAAT TGCTCCATGG GGCATCGTGG AGAATAAGGA AGACCTGGTT 
GGAAAGGATG TAACAAGAGT GTACCAGACC ATGTCCAACC CTCTAAGTAA GCTCTCTGTG 
CTCAACAACT CCCACACCCA CTTCATCCTG GCTGACAATG GCACCCTGGG CAAGTATGGC 
GCCGAGGTGA AGCTGCGAAG GCTGCTGGAA AAGCACATCT CCCTGCAGAA GATCAACACA 
AGACTGGGGC AGGGCGTGCC CCTCGTGGGT CTCGTGGTGG AGGGGGGCCC TAACGTGGTG 
TCCATCGTCT TGGAATACCT GCAAGAAGAG CCTCCCATCC CTGTGGTGAT TTGTGATGGC 
AGCGGACGTG CCTCGGACAT CCTGTCCTTT GCGCACAAGT ACTGTGAAGA AGGCGGAATA 
ATAAATGAGT CCCTCAGGGA GCAGCTTCTA GTTACCATTC AGAAAACATT TAATTATAAT 
AAGGCACAAT CACATCAGCT GTTTGCAATT ATAATGGAGT GCATGAAGAA GAAAGAACTC 
GTCACTGTGT TCAGAATGGG TTCTGAGGGC CAGCAGGACA TCGAGATGGC AATTTTAACT 
GCCCTGCTGA AAGGAACAAA CGTATCTGCT CCAGATCAGC TGAGCTTGGC ACTGGCTTGG 
AACCGCGTGG ACATAGCACG AAGCCAGATC TTTGTCTTTG GGCCCCACTG GCCGCCCCTG 
GGAAGCCTGG CACCCCCGAC GGACAGCAAA GCCACGGAGA AGGAGAAGAA GCCACCCATG 
GCCACCACCA AGGGAGGAAG AGGAAAAGGG AAAGGCAAGA AGAAAGGGAA AGTGAAAGAG 
GAAGTGGAGG AAGAAACTGA CCCCCGGAAG ATAGAGCTGC TGAACTGGGT GAATGCTTTG 
GAGCAAGCGA TGCTAGATGC TTTAGTCTTA GATCGTGTCG ACTTTGTGAA GCTCCTGATT 
GAAAACGGAG TGAACATGCA ACACTTTCTG ACCATTCCGA GGCTGGAGGA GCTTTATAAC 
ACAAGACTGG GTCCACCAAA CACACTTCAT CTGCTGGTGA GGGATGTGAA AAAGAGCAAC 
CTTCCGCCTG ATTACCACAT CAGCCTCATA GACATCGGGC TCGTGCTGGA GTACCTCATG 
GGAGGAGCCT ACCGCTGCAA CTACACTCGG AAAAACTTTC GGACCCTTTA CAACAACTTG 
TTTGGACCAA AGAGGCCTAA AGCTCTTAAA CTTCTGGGAA TGGAAGATGA TGAGCCTCCA 
GCTAAAGGGA AGAAAAAGAA AAAGAAGAAA AAGGAGGAAG AGATCGACAT TGATGTGGAC 
GACCCTGCCG TGAGTCGGTT CCAGTATCCC TTCCACGAGC TGATGGTGTG GGCAGTGCTG 
ATGAAACGCC AGAAAATGGC AGTGTTCCTC TGGCAGCGAG GGGAAGAGAG CATGGCCAAG 
GCCCTGGTGG CCTGCAAGCT CTACAAGGCC ATGGCCCACG AGTCCTCCGA GAGTGATCTG 
GTGGATGACA TCTCCCAGGA CTTGGATAAC AATTCCAAAG ACTTCGGCCA GCTTGCTTTG 
GAGTTATTAG ACCAGTCCTA TAAGCATGAC GAGCAGATCG CTATGAAACT CCTGACCTAC 
GAGCTGAAAA ACTGGAGCAA CTCGACCTGC CTCAAACTGG CCGTGGCAGC CAAACACCGG 
GACTTCATTG CTCACACCTG CAGCCAGATG CTGCTGACCG ATATGTGGAT GGGAAGACTG 
CGGATGCGGA AGAACCCCGG CCTGAAGGTT ATCATGGGGA TTCTTCTACC CCCCACCATC 
TTGTTTTTGG AATTTCGCAC ATATGATGAT TTCTCGTATC AAACATCCAA GGAAAATGAG 
GATGGCAAAG AAAAAGAAGA GGAAAATACG GATGCAAATG CAGATGCTGG CTCAAGAAAG 
GGGGATGAGG AGAACGAGCA CAAAAAACAG AGAAGTATTC CCATCGGAAC AAAGATCTGT 
GAATTCTATA ACGCGCCCAT TGTCAAGTTC TGGTTTTACA CAATATCATA CTTGGGCTAC 
CTGCTGCTGT TTAACTACGT CATCCTGGTG CGGATGGATG GCTGGCCGTC CCTCCAGGAG 
TGGATCGTCA TCTCCTACAT CGTGAGCCTG GCGTTAGAGA AGATACGAGA GATCCTCATG 
TCAGAACCAG GCAAACTCAG CCAGAAAATC AAAGTTTGGC TTCAGGAGTA CTGGAACATC 
ACAGATCTCG TGGCCATTTC CACATTCATG ATTGGAGCAA TTCTTCGCCT ACAGAACCAG 
CCCTACATGG GCTATGGCCG GGTGATCTAC TGTGTGGATA TCATCTTCTG GTACATCCGT 
GTCCTGGACA TCTTTGGTGT CAACAAGTAT CTGGGGCCAT ACGTGATGAT GATTGGAAAG 
ATGATGATCG ACATGCTGTA CTTTGTGGTC ATCATGCTGG TCGTGCTCAT GAGTTTCGGA 
GTAGCCCGTC AAGCCATTCT GCATCCAGAG GAGAAGCCCT CTTGGAAACT GGCCCGAAAC 
ATCTTCTACA TGCCCTACTG GATGATCTAT GGAGAGGTGT TTGCAGACCA GATAGACCTC 
TACGCCATGG AAATTAATCC TCCTTGTGGT GAGAACCTAT ATGATGAGGA GGGCAAGCGG 
CTTCCTCCCT GTATCCCCGG CGCCTGGCTC ACTCCAGCAC TCATGGCGTG CTATCTACTG 
GTCGCCAACA TCCTGCTGGT GAACCTGCTG ATTGCTGTGT TCAACAATAC CTTCTTTGAA 
GTAAAATCAA TATCCAACCA GGTGTGGAAG TTCCAGCGAT ATCAGCTGAT TATGACATTT 
CATGACAGGC CAGTCCTGCC CCCACCGATG ATCATTTTAA GCCACATCTA CATCATCATT 
ATGCGTCTCA GCGGCCGCTG CAGGAAAAAG AGAGAAGGGG ACCAAGAGGA ACGGGATCGT 
GGATTGAAGC TCTTCCTTAG CGACGAGGAG CTAAAGAGGC TGCATGAGTT CGAGGAGCAG 
TGCGTGCAGG AGCACTTCCG GGAGAAGGAG GATGAGCAGC AGTCGTCCAG CGACGAGCGC 
ATCCGGGTCA CTTCTGAAAG AGTTGAAAAT ATGTCAATGA GGTTGGAAGA AATCAATGAA 
AGAGAAACTT TTATGAAAAC TTCCCTGCAG ACTGTTGACC TTCGACTTGC TCAGCTAGAA 
GAATTATCTA ACAGAATGGT GAATGCTCTT GAAAATCTTG CGGGAATCGA CAGGTCTGAC 
CTGATCCAGG CACGGTCCCG GGCTTCTTCT GAATGTGAGG CAACGTATCT TCTCCGGCAA 
AGCAGCATCA ATAGCGCTGA TGGCTACAGC TTGTATCGAT ATCATTTTAA CGGAGAAGAG 
TTATTATTTG AGGATACATC TCTCTCCACG TCACCAGGGA CAGGAGTCAG GAAAAAAACC 
TGTTCCTTCC GTATAAAGGA AGAGAAGGAC GTGAAAACGC ACCTAGTCCC AGAATGTCAG 
AACAGTCTTC ACCTTTCACT GGGCACAAGC ACATCAGCAA CCCCAGATGG CAGTCACCTT 
GCAGTAGATG ACTTAAAGAA CGCTGAAGAG TCAAAATTAG GTCCAGATAT TGGGATTTCA 
AAGGAAGATG ATGAAAGACA GACAGACTCT AAAAAAGAAG AAACTATTTC CCCAAGTTTA 
AATAAAACAG ATGTGATACA TGGACAGGAC AAATCAGATG TTCAAAACAC TCAGCTAACA 
GTGGAAACGA CAAATATAGA AGGCACTATT TCCTATCCCC TGGAAGAAAC CAAAATTACA 
CGCTATTTCC CCGATGAAAC GATCAATGCT TGTAAAACAA TGAAGTCCAG AAGCTTCGTC 
TATTCCCGGG GAAGAAAGCT GGTCGGTGGG GTTAACCAGG ATGTAGAGTA CAGTTCAATC 
ACGGACCAGC AATTGACGAC GGAATGGCAA TGCCAAGTTC AAAAGATCAC GCGCTCTCAT 
AGCACAGATA TTCCTTACAT TGTGTCGGAA GCTGCAGTGC AAGCTGAGCA TAAAGAGCAG 
TTTGCAGATA TGCAAGATGA ACACCATGTC GCTGAAGCAA TTCCTCGAAT CCCTCGCTTG 
TCCCTAACCA TTACTGACAG AAATGGGATG GAAAACTTAC TGTCTGTGAA GCCAGATCAA 
ACTTTGGGAT TCCCATCTCT CAGGTCAAAA AGTTTACATG GACATCCTAG GAATGTGAAA 
TCCATTCAGG GAAAGTTAGA CAGATCTGGA CATGCCAGTA GTGTAAGCAG CTTAGTAATT 
GTGTCTGGAA TGACAGCAGA AGAAAAAAAG GTTAAGAAAG AGAAAGCTTC CACAGAAACT 
GAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238949.1
CDS	315..5243
Translation	MSSFKRGSLKSSTSGSQKGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC

Target ORF information:

RefSeq Version	NM_001252020.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252020.1

ORF Insert Sequence:

ATGAGCTCCT TCAAGCGGGG CTCGCTCAAG AGCTCCACAT CAGGGTCGCA GAAGGGTCAG 
AAATCTTGGA TAGAGAAAAC CTTTTGCAAA CGGGAATGTA TCTTTGTAAT TCCTAGCATG 
AAAGACTCTA ACAGGTGTTG CTGTGGCCAG TTCACCAACC AGCATATCCC CCCTCTGCCA 
AGTGCAACAC CCAGCAAAAA TGAAGAGGAA AGCAAACAGG TGGAGACTCA GCCTGAGAAA 
TGGTCTGTTG CCAAGCACAC CCAGAGCTAC CCAACAGATT CCTATGGAGT TCTTGAATTC 
CAGGGTGGCG GATATTCCAA TAAAGCCATG TATATCCGTG TATCCTATGA CACCAAGCCA 
GACTCACTGC TCCATCTCAT GGTGAAAGAT TGGCAGCTGG AACTCCCCAA GCTCTTAATA 
TCTGTGCATG GAGGCCTCCA GAACTTTGAG ATGCAGCCCA AGCTGAAACA AGTCTTTGGG 
AAAGGCCTGA TCAAGGCTGC TATGACCACC GGGGCCTGGA TCTTCACCGG GGGTGTCAGC 
ACAGGTGTTA TCAGCCACGT AGGGGATGCC TTGAAAGACC ACTCCTCCAA GTCCAGAGGC 
CGGGTTTGTG CTATAGGAAT TGCTCCATGG GGCATCGTGG AGAATAAGGA AGACCTGGTT 
GGAAAGGATG TAACAAGAGT GTACCAGACC ATGTCCAACC CTCTAAGTAA GCTCTCTGTG 
CTCAACAACT CCCACACCCA CTTCATCCTG GCTGACAATG GCACCCTGGG CAAGTATGGC 
GCCGAGGTGA AGCTGCGAAG GCTGCTGGAA AAGCACATCT CCCTGCAGAA GATCAACACA 
AGACTGGGGC AGGGCGTGCC CCTCGTGGGT CTCGTGGTGG AGGGGGGCCC TAACGTGGTG 
TCCATCGTCT TGGAATACCT GCAAGAAGAG CCTCCCATCC CTGTGGTGAT TTGTGATGGC 
AGCGGACGTG CCTCGGACAT CCTGTCCTTT GCGCACAAGT ACTGTGAAGA AGGCGGAATA 
ATAAATGAGT CCCTCAGGGA GCAGCTTCTA GTTACCATTC AGAAAACATT TAATTATAAT 
AAGGCACAAT CACATCAGCT GTTTGCAATT ATAATGGAGT GCATGAAGAA GAAAGAACTC 
GTCACTGTGT TCAGAATGGG TTCTGAGGGC CAGCAGGACA TCGAGATGGC AATTTTAACT 
GCCCTGCTGA AAGGAACAAA CGTATCTGCT CCAGATCAGC TGAGCTTGGC ACTGGCTTGG 
AACCGCGTGG ACATAGCACG AAGCCAGATC TTTGTCTTTG GGCCCCACTG GCCGCCCCTG 
GGAAGCCTGG CACCCCCGAC GGACAGCAAA GCCACGGAGA AGGAGAAGAA GCCACCCATG 
GCCACCACCA AGGGAGGAAG AGGAAAAGGG AAAGGCAAGA AGAAAGGGAA AGTGAAAGAG 
GAAGTGGAGG AAGAAACTGA CCCCCGGAAG ATAGAGCTGC TGAACTGGGT GAATGCTTTG 
GAGCAAGCGA TGCTAGATGC TTTAGTCTTA GATCGTGTCG ACTTTGTGAA GCTCCTGATT 
GAAAACGGAG TGAACATGCA ACACTTTCTG ACCATTCCGA GGCTGGAGGA GCTTTATAAC 
ACAAGACTGG GTCCACCAAA CACACTTCAT CTGCTGGTGA GGGATGTGAA AAAGAGCAAC 
CTTCCGCCTG ATTACCACAT CAGCCTCATA GACATCGGGC TCGTGCTGGA GTACCTCATG 
GGAGGAGCCT ACCGCTGCAA CTACACTCGG AAAAACTTTC GGACCCTTTA CAACAACTTG 
TTTGGACCAA AGAGGCCTAA AGCTCTTAAA CTTCTGGGAA TGGAAGATGA TGAGCCTCCA 
GCTAAAGGGA AGAAAAAGAA AAAGAAGAAA AAGGAGGAAG AGATCGACAT TGATGTGGAC 
GACCCTGCCG TGAGTCGGTT CCAGTATCCC TTCCACGAGC TGATGGTGTG GGCAGTGCTG 
ATGAAACGCC AGAAAATGGC AGTGTTCCTC TGGCAGCGAG GGGAAGAGAG CATGGCCAAG 
GCCCTGGTGG CCTGCAAGCT CTACAAGGCC ATGGCCCACG AGTCCTCCGA GAGTGATCTG 
GTGGATGACA TCTCCCAGGA CTTGGATAAC AATTCCAAAG ACTTCGGCCA GCTTGCTTTG 
GAGTTATTAG ACCAGTCCTA TAAGCATGAC GAGCAGATCG CTATGAAACT CCTGACCTAC 
GAGCTGAAAA ACTGGAGCAA CTCGACCTGC CTCAAACTGG CCGTGGCAGC CAAACACCGG 
GACTTCATTG CTCACACCTG CAGCCAGATG CTGCTGACCG ATATGTGGAT GGGAAGACTG 
CGGATGCGGA AGAACCCCGG CCTGAAGGTT ATCATGGGGA TTCTTCTACC CCCCACCATC 
TTGTTTTTGG AATTTCGCAC ATATGATGAT TTCTCGTATC AAACATCCAA GGAAAATGAG 
GATGGCAAAG AAAAAGAAGA GGAAAATACG GATGCAAATG CAGATGCTGG CTCAAGAAAG 
GGGGATGAGG AGAACGAGCA CAAAAAACAG AGAAGTATTC CCATCGGAAC AAAGATCTGT 
GAATTCTATA ACGCGCCCAT TGTCAAGTTC TGGTTTTACA CAATATCATA CTTGGGCTAC 
CTGCTGCTGT TTAACTACGT CATCCTGGTG CGGATGGATG GCTGGCCGTC CCTCCAGGAG 
TGGATCGTCA TCTCCTACAT CGTGAGCCTG GCGTTAGAGA AGATACGAGA GATCCTCATG 
TCAGAACCAG GCAAACTCAG CCAGAAAATC AAAGTTTGGC TTCAGGAGTA CTGGAACATC 
ACAGATCTCG TGGCCATTTC CACATTCATG ATTGGAGCAA TTCTTCGCCT ACAGAACCAG 
CCCTACATGG GCTATGGCCG GGTGATCTAC TGTGTGGATA TCATCTTCTG GTACATCCGT 
GTCCTGGACA TCTTTGGTGT CAACAAGTAT CTGGGGCCAT ACGTGATGAT GATTGGAAAG 
ATGATGATCG ACATGCTGTA CTTTGTGGTC ATCATGCTGG TCGTGCTCAT GAGTTTCGGA 
GTAGCCCGTC AAGCCATTCT GCATCCAGAG GAGAAGCCCT CTTGGAAACT GGCCCGAAAC 
ATCTTCTACA TGCCCTACTG GATGATCTAT GGAGAGGTGT TTGCAGACCA GATAGACCTC 
TACGCCATGG AAATTAATCC TCCTTGTGGT GAGAACCTAT ATGATGAGGA GGGCAAGCGG 
CTTCCTCCCT GTATCCCCGG CGCCTGGCTC ACTCCAGCAC TCATGGCGTG CTATCTACTG 
GTCGCCAACA TCCTGCTGGT GAACCTGCTG ATTGCTGTGT TCAACAATAC CTTCTTTGAA 
GTAAAATCAA TATCCAACCA GGTGTGGAAG TTCCAGCGAT ATCAGCTGAT TATGACATTT 
CATGACAGGC CAGTCCTGCC CCCACCGATG ATCATTTTAA GCCACATCTA CATCATCATT 
ATGCGTCTCA GCGGCCGCTG CAGGAAAAAG AGAGAAGGGG ACCAAGAGGA ACGGGATCGT 
GGATTGAAGC TCTTCCTTAG CGACGAGGAG CTAAAGAGGC TGCATGAGTT CGAGGAGCAG 
TGCGTGCAGG AGCACTTCCG GGAGAAGGAG GATGAGCAGC AGTCGTCCAG CGACGAGCGC 
ATCCGGGTCA CTTCTGAAAG AGTTGAAAAT ATGTCAATGA GGTTGGAAGA AATCAATGAA 
AGAGAAACTT TTATGAAAAC TTCCCTGCAG ACTGTTGACC TTCGACTTGC TCAGCTAGAA 
GAATTATCTA ACAGAATGGT GAATGCTCTT GAAAATCTTG CGGGAATCGA CAGGTCTGAC 
CTGATCCAGG CACGGTCCCG GGCTTCTTCT GAATGTGAGG CAACGTATCT TCTCCGGCAA 
AGCAGCATCA ATAGCGCTGA TGGCTACAGC TTGTATCGAT ATCATTTTAA CGGAGAAGAG 
TTATTATTTG AGGATACATC TCTCTCCACG TCACCAGGGA CAGGAGTCAG GAAAAAAACC 
TGTTCCTTCC GTATAAAGGA AGAGAAGGAC GTGAAAACGC ACCTAGTCCC AGAATGTCAG 
AACAGTCTTC ACCTTTCACT GGGCACAAGC ACATCAGCAA CCCCAGATGG CAGTCACCTT 
GCAGTAGATG ACTTAAAGAA CGCTGAAGAG TCAAAATTAG GTCCAGATAT TGGGATTTCA 
AAGGAAGATG ATGAAAGACA GACAGACTCT AAAAAAGAAG AAACTATTTC CCCAAGTTTA 
AATAAAACAG ATGTGATACA TGGACAGGAC AAATCAGATG TTCAAAACAC TCAGCTAACA 
GTGGAAACGA CAAATATAGA AGGCACTATT TCCTATCCCC TGGAAGAAAC CAAAATTACA 
CGCTATTTCC CCGATGAAAC GATCAATGCT TGTAAAACAA TGAAGTCCAG AAGCTTCGTC 
TATTCCCGGG GAAGAAAGCT GGTCGGTGGG GTTAACCAGG ATGTAGAGTA CAGTTCAATC 
ACGGACCAGC AATTGACGAC GGAATGGCAA TGCCAAGTTC AAAAGATCAC GCGCTCTCAT 
AGCACAGATA TTCCTTACAT TGTGTCGGAA GCTGCAGTGC AAGCTGAGCA TAAAGAGCAG 
TTTGCAGATA TGCAAGATGA ACACCATGTC GCTGAAGCAA TTCCTCGAAT CCCTCGCTTG 
TCCCTAACCA TTACTGACAG AAATGGGATG GAAAACTTAC TGTCTGTGAA GCCAGATCAA 
ACTTTGGGAT TCCCATCTCT CAGGTCAAAA AGTTTACATG GACATCCTAG GAATGTGAAA 
TCCATTCAGG GAAAGTTAGA CAGATCTGGA CATGCCAGTA GTGTAAGCAG CTTAGTAATT 
GTGTCTGGAA TGACAGCAGA AGAAAAAAAG GTTAAGAAAG AGAAAGCTTC CACAGAAACT 
GAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM1 ( NM_001252030.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238959.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.

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CloneID	OHu14795
Clone ID Related Accession (Same CDS sequence)	NM_001252030.2 , NM_001252030.1
Accession Version	NM_001252030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, AC009562.9 and BU741333.1. On Jun 2, 2019 this sequence version replaced NM_001252030.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070356.1, BU933033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC 
ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT 
CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT 
CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238959.1
CDS	133..543
Translation	MKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMVRKAFRHGATRITAFIGGQSPSPKLQIPGLLHGCGSIFLDISLKNQEIYLCTWLLAMRLGNWTPL

Target ORF information:

RefSeq Version	NM_001252030.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252030.2

ORF Insert Sequence:

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC 
ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT 
CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT 
CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM1 ( NM_001252030.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238959.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu14795
Clone ID Related Accession (Same CDS sequence)	NM_001252030.2 , NM_001252030.1
Accession Version	NM_001252030.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, AC009562.9 and BU741333.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070356.1, BC058286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC 
ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT 
CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT 
CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238959.1
CDS	134..544
Translation	MKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMVRKAFRHGATRITAFIGGQSPSPKLQIPGLLHGCGSIFLDISLKNQEIYLCTWLLAMRLGNWTPL

Target ORF information:

RefSeq Version	NM_001252030.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252030.1

ORF Insert Sequence:

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC 
ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT 
CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT 
CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM1 ( NM_001252024.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238953.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.

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CloneID	OHu14262
Clone ID Related Accession (Same CDS sequence)	NM_001252024.2 , NM_001252024.1
Accession Version	NM_001252024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, HM135790.1, AC009562.9 and BU743036.1. On Jun 2, 2019 this sequence version replaced NM_001252024.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate exon in the 5' coding region. These differences cause translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM135790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC 
CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG 
CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT 
CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC 
ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG 
CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA 
GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG 
GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG 
TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA 
GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG 
CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC 
AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG 
ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT 
AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT 
TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA 
GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT 
AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG 
AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA 
ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA 
CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG 
CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG 
CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA 
GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG 
AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG 
CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG 
CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA 
AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG 
TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC 
AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT 
GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT 
GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG 
GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC 
ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG 
AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG 
CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC 
CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC 
AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG 
GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC 
CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG 
GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC 
TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA 
AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC 
TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC 
CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG 
ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC 
TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA 
CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG 
TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG 
ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG 
AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG 
GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG 
ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG 
GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC 
TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC 
TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT 
ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC 
ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA 
CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC 
GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC 
GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA 
ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT 
CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC 
AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT 
CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC 
GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG 
AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA 
GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC 
AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT 
GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC 
CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT 
CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC 
AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA 
AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC 
AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG 
CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT 
AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC 
CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG 
CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG 
AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC 
TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC 
ACAGAAACTG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238953.1
CDS	153..5030
Translation	MGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC

Target ORF information:

RefSeq Version	NM_001252024.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252024.2

ORF Insert Sequence:

ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC 
CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG 
CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT 
CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC 
ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG 
CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA 
GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG 
GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG 
TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA 
GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG 
CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC 
AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG 
ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT 
AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT 
TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA 
GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT 
AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG 
AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA 
ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA 
CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG 
CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG 
CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA 
GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG 
AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG 
CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG 
CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA 
AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG 
TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC 
AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT 
GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT 
GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG 
GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC 
ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG 
AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG 
CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC 
CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC 
AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG 
GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC 
CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG 
GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC 
TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA 
AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC 
TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC 
CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG 
ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC 
TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA 
CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG 
TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG 
ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG 
AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG 
GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG 
ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG 
GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC 
TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC 
TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT 
ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC 
ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA 
CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC 
GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC 
GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA 
ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT 
CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC 
AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT 
CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC 
GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG 
AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA 
GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC 
AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT 
GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC 
CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT 
CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC 
AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA 
AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC 
AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG 
CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT 
AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC 
CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG 
CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG 
AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC 
TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC 
ACAGAAACTG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM1 ( NM_001252024.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238953.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.

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CloneID	OHu14262
Clone ID Related Accession (Same CDS sequence)	NM_001252024.2 , NM_001252024.1
Accession Version	NM_001252024.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, HM135790.1, AC009562.9 and BU743036.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate exon in the 5' coding region. These differences cause translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM135790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC 
CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG 
CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT 
CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC 
ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG 
CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA 
GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG 
GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG 
TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA 
GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG 
CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC 
AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG 
ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT 
AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT 
TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA 
GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT 
AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG 
AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA 
ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA 
CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG 
CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG 
CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA 
GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG 
AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG 
CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG 
CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA 
AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG 
TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC 
AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT 
GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT 
GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG 
GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC 
ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG 
AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG 
CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC 
CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC 
AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG 
GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC 
CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG 
GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC 
TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA 
AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC 
TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC 
CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG 
ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC 
TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA 
CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG 
TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG 
ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG 
AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG 
GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG 
ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG 
GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC 
TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC 
TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT 
ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC 
ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA 
CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC 
GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC 
GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA 
ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT 
CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC 
AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT 
CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC 
GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG 
AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA 
GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC 
AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT 
GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC 
CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT 
CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC 
AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA 
AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC 
AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG 
CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT 
AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC 
CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG 
CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG 
AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC 
TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC 
ACAGAAACTG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238953.1
CDS	154..5031
Translation	MGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC

Target ORF information:

RefSeq Version	NM_001252024.1
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252024.1

ORF Insert Sequence:

ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC 
CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG 
CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT 
CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC 
ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG 
CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA 
GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG 
GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG 
TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA 
GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG 
CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC 
AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG 
ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT 
AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT 
TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA 
GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT 
AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG 
AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA 
ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA 
CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG 
CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG 
CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA 
GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG 
AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG 
CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG 
CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA 
AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG 
TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC 
AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT 
GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT 
GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG 
GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC 
ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG 
AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG 
CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC 
CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC 
AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG 
GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC 
CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG 
GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC 
TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA 
AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC 
TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC 
CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG 
ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC 
TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA 
CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG 
TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG 
ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG 
AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG 
GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG 
ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG 
GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC 
TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC 
TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT 
ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC 
ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA 
CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC 
GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC 
GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA 
ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT 
CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC 
AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT 
CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC 
GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG 
AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA 
GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC 
AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT 
GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC 
CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT 
CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC 
AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA 
AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC 
AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG 
CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT 
AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC 
CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG 
CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG 
AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC 
TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC 
ACAGAAACTG AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM1 ( NM_002420.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002411.3 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20829
Clone ID Related Accession (Same CDS sequence)	NM_002420.6 , NM_002420.5
Accession Version	NM_002420.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU933033.1, AF071787.1, AC009562.9, HM135791.1 and BU743036.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_002420.5. Transcript Variant: This variant (2) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF071787.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG 
CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA 
ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT 
GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC 
AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA 
GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG 
GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT 
GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT 
GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC 
ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG 
GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT 
GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA 
ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT 
AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA 
CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA 
ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT 
TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC 
CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC 
ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA 
GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT 
TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG 
ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT 
AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC 
AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC 
ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC 
TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT 
CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG 
GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG 
CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC 
AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT 
CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT 
TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC 
TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC 
CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA 
CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC 
ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT 
GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA 
AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC 
TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC 
TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG 
GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC 
ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC 
ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC 
CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC 
CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA 
AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC 
GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA 
AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC 
CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG 
CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA 
CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT 
GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA 
TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC 
ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT 
CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG 
CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG 
CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT 
GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA 
GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT 
GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG 
CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA 
GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA 
ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT 
CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC 
CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT 
TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT 
TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA 
ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT 
ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC 
GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA 
ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT 
CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG 
CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC 
TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT 
CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG 
AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA 
ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA 
ACTGAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002411.3
CDS	133..4944
Translation	MKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC

Target ORF information:

RefSeq Version	NM_002420.6
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002420.6

ORF Insert Sequence:

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG 
CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA 
ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT 
GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC 
AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA 
GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG 
GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT 
GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT 
GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC 
ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG 
GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT 
GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA 
ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT 
AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA 
CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA 
ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT 
TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC 
CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC 
ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA 
GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT 
TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG 
ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT 
AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC 
AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC 
ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC 
TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT 
CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG 
GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG 
CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC 
AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT 
CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT 
TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC 
TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC 
CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA 
CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC 
ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT 
GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA 
AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC 
TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC 
TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG 
GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC 
ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC 
ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC 
CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC 
CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA 
AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC 
GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA 
AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC 
CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG 
CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA 
CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT 
GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA 
TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC 
ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT 
CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG 
CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG 
CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT 
GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA 
GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT 
GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG 
CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA 
GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA 
ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT 
CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC 
CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT 
TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT 
TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA 
ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT 
ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC 
GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA 
ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT 
CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG 
CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC 
TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT 
CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG 
AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA 
ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA 
ACTGAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM1 ( NM_002420.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002411.3 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20829
Clone ID Related Accession (Same CDS sequence)	NM_002420.6 , NM_002420.5
Accession Version	NM_002420.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	transient receptor potential cation channel subfamily M member 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU933033.1, AF071787.1, AC009562.9, HM135791.1 and BU743036.1. This sequence is a reference standard in the RefSeqGene project. On or before Mar 22, 2015 this sequence version replaced XM_011548920.1, XM_011547404.1, XM_006725544.1, NM_002420.4. Transcript Variant: This variant (2) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF071787.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG 
CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA 
ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT 
GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC 
AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA 
GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG 
GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT 
GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT 
GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC 
ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG 
GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT 
GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA 
ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT 
AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA 
CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA 
ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT 
TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC 
CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC 
ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA 
GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT 
TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG 
ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT 
AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC 
AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC 
ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC 
TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT 
CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG 
GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG 
CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC 
AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT 
CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT 
TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC 
TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC 
CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA 
CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC 
ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT 
GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA 
AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC 
TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC 
TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG 
GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC 
ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC 
ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC 
CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC 
CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA 
AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC 
GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA 
AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC 
CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG 
CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA 
CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT 
GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA 
TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC 
ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT 
CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG 
CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG 
CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT 
GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA 
GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT 
GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG 
CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA 
GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA 
ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT 
CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC 
CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT 
TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT 
TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA 
ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT 
ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC 
GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA 
ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT 
CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG 
CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC 
TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT 
CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG 
AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA 
ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA 
ACTGAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002411.3
CDS	134..4945
Translation	MKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC

Target ORF information:

RefSeq Version	NM_002420.5
Organism	Homo sapiens(human)
Definition	Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002420.5

ORF Insert Sequence:

ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG 
AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA 
TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG 
CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA 
ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT 
GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC 
AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA 
GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG 
GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT 
GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT 
GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC 
ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG 
GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT 
GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA 
ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT 
AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA 
CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA 
ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT 
TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC 
CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC 
ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA 
GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT 
TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG 
ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT 
AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC 
AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC 
ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC 
TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT 
CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG 
GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG 
CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC 
AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT 
CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT 
TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC 
TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC 
CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA 
CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC 
ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT 
GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA 
AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC 
TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC 
TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG 
GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC 
ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC 
ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC 
CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC 
CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA 
AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC 
GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA 
AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC 
CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG 
CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA 
CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT 
GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA 
TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC 
ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT 
CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG 
CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG 
CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT 
GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA 
GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT 
GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG 
CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA 
GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA 
ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT 
CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC 
CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT 
TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT 
TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA 
ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT 
ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC 
GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA 
ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT 
CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG 
CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC 
TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT 
CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG 
AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA 
ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA 
ACTGAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Founder Ashkenazi Jewish mutations of large deletion in the inherited retinal dystrophy genes.
Ophthalmic genetics39(1)135-136(2018 Jan-Feb)
Chiang JP,Luo H,Duan J,Ekstein J,Hirsch Y

Joint Analysis of Nuclear and Mitochondrial Variants in Age-Related Macular Degeneration Identifies Novel Loci TRPM1 and ABHD2/RLBP1.
Investigative ophthalmology & visual science58(10)4027-4038(2017 Aug)
Persad PJ,Heid IM,Weeks DE,Baird PN,de Jong EK,Haines JL,Pericak-Vance MA,Scott WK,

Structural basis of dual Ca
Nature structural & molecular biology24(3)205-213(2017 Mar)
Li M,Zhang WK,Benvin NM,Zhou X,Su D,Li H,Wang S,Michailidis IE,Tong L,Li X,Yang J

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TRPM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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