CDK3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDK3
Entrez Gene ID	1018
Full Name	cyclin dependent kinase 3
General protein information	Preferred Names cyclin dependent kinase 3 Names cyclin-dependent kinase 3 cell division protein kinase 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.1
Summary	This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008].
Disorder MIM:	123828

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258.2	NP_001249.1	cyclin-dependent kinase 3

Pathways from BioSystems

Homologies

	CDK3	XP_001102314.2
Pan troglodytes (chimpanzee)	CDK3	XP_523720.2
Bos taurus (cattle)	CDK3	NP_001092648.1
Homo sapiens (human)	CDK3	NP_001249.1
Canis lupus familiaris (dog)	CDK3	XP_540442.3
Gallus gallus (chicken)	CDK3	NP_001075175.2

Gene Ontology
Bibliography

Related articles in PubMed

HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis.
Zhang Y, Yang L, Ling C, Heng W
Biotechnology letters(2018 Jan)

miR-125a-3p inhibits ER? transactivation and overrides tamoxifen resistance by targeting CDK3 in estrogen receptor-positive breast cancer.
Zheng L, Meng X, Li X, Zhang Y, Li C, Xiang C, Xing Y, Xia Y, Xi T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Sep)

HuR promotes breast cancer cell proliferation and survival via binding to CDK3 mRNA.
Zhang Z, Huang A, Zhang A, Zhou C
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie91788-795(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect.
Wang L, Xi Y, Sun C, Zhang F, Jiang H, He Q, Li D
Experimental and molecular pathology102(2)181-190(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results provided evidence supporting the oncogenic potential of NFAT3 and suggested that CDK3-mediated phosphorylation of NFAT3 has an important role in skin tumorigenesis.
Title: Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer.

The analysis of tumor and matched normal lung tissues indicates that miR-150 downregulation in lung tumors correlates with higher CDK3 levels. In addition, miR-150 transfection experiments with cancer-derived cell lines reveal that miR-150-mediated CDK3 suppression directly induces growth inhibition.
Title: CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect.

High Cdk3-promoted epithelial-mesenchymal transition through activating AP-1 is involved in colorectal cancer metastasis.
Title: Cdk3-promoted epithelial-mesenchymal transition through activating AP-1 is involved in colorectal cancer metastasis.

Data indicate that microRNA miR-214 has tumor-suppressive activity in hepatocellular carcinoma (HCC) through inhibition of E2F2 transcription factor (E2F2), cyclin-dependent kinases CDK3 and CDK6.
Title: miR-214/199a/199a* cluster levels predict poor survival in hepatocellular carcinoma through interference with cell-cycle regulators.

Mir-873 inhibits ESR1 activity and cell growth via targeting CDK3.
Title: MiR-873 regulates ER? transcriptional activity and tamoxifen resistance via targeting CDK3 in breast cancer cells.

The following CDK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu31943	NM_001258.2 Latest version!	Homo sapiens cyclin dependent kinase 3 (CDK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDK3 ( NM_001258.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001249.1 Homo sapiens cyclin dependent kinase 3 (CDK3), mRNA.

Price & Availability↑

CloneID	OHu31943
Clone ID Related Accession (Same CDS sequence)	NM_001258.2
Accession Version	NM_001258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	cyclin-dependent kinase 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB455174.1, X66357.1, BU784937.1, AU146328.1 and BX098771.1. On Jan 9, 2008 this sequence version replaced NM_001258.1. Sequence Note: A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species and on a strong Kozak signal. An upstream in-frame start codon is also present but has a weaker Kozak signal and is poorly conserved. Use of the upstream start codon would result in a protein that is 20 aa longer at the N-terminus. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK097104.1, SRR1803611.219851.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATATGT TCCAGAAGGT AGAGAAGATC GGAGAGGGCA CCTATGGGGT GGTGTACAAG 
GCCAAGAACA GGGAGACAGG GCAGCTGGTG GCCCTGAAGA AGATCAGACT GGATTTGGAG 
ATGGAGGGGG TCCCAAGCAC TGCCATCAGG GAGATCTCGC TGCTCAAGGA ACTGAAGCAC 
CCCAACATCG TCCGACTGCT GGACGTGGTG CACAACGAGA GGAAGCTCTA TCTGGTGTTT 
GAGTTCCTCA GCCAGGACCT GAAGAAGTAC ATGGACTCCA CCCCAGGCTC AGAGCTCCCC 
CTGCACCTCA TCAAGAGCTA CCTCTTCCAG CTGCTGCAGG GGGTGAGTTT CTGCCACTCA 
CATCGGGTCA TCCACCGAGA CCTGAAGCCC CAGAACCTGC TCATCAATGA GTTGGGTGCC 
ATCAAGCTGG CTGACTTCGG CCTGGCTCGC GCCTTCGGGG TGCCCCTGCG CACCTACACC 
CATGAGGTGG TGACACTGTG GTATCGCGCC CCCGAGATTC TCTTGGGCAG CAAGTTCTAT 
ACCACAGCTG TGGATATCTG GAGCATTGGT TGCATCTTTG CAGAGATGGT GACTCGAAAA 
GCCCTGTTTC CTGGTGACTC TGAGATTGAC CAGCTCTTTC GTATCTTTCG TATGCTGGGG 
ACACCCAGCG AAGACACATG GCCCGGGGTC ACCCAGCTGC CTGACTATAA GGGCAGCTTC 
CCTAAGTGGA CCAGGAAGGG ACTGGAAGAG ATTGTGCCCA ATCTGGAGCC AGAGGGCAGG 
GACCTGCTCA TGCAACTCCT GCAGTATGAC CCCAGCCAGC GGATCACAGC CAAGACTGCC 
CTGGCCCACC CGTACTTCTC ATCCCCTGAG CCCTCCCCAG CTGCCCGCCA GTATGTGCTG 
CAGCGATTCC GCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001249.1
CDS	77..994
Translation	MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH

Target ORF information:

RefSeq Version	NM_001258.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclin dependent kinase 3 (CDK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258.2

ORF Insert Sequence:

ATGGATATGT TCCAGAAGGT AGAGAAGATC GGAGAGGGCA CCTATGGGGT GGTGTACAAG 
GCCAAGAACA GGGAGACAGG GCAGCTGGTG GCCCTGAAGA AGATCAGACT GGATTTGGAG 
ATGGAGGGGG TCCCAAGCAC TGCCATCAGG GAGATCTCGC TGCTCAAGGA ACTGAAGCAC 
CCCAACATCG TCCGACTGCT GGACGTGGTG CACAACGAGA GGAAGCTCTA TCTGGTGTTT 
GAGTTCCTCA GCCAGGACCT GAAGAAGTAC ATGGACTCCA CCCCAGGCTC AGAGCTCCCC 
CTGCACCTCA TCAAGAGCTA CCTCTTCCAG CTGCTGCAGG GGGTGAGTTT CTGCCACTCA 
CATCGGGTCA TCCACCGAGA CCTGAAGCCC CAGAACCTGC TCATCAATGA GTTGGGTGCC 
ATCAAGCTGG CTGACTTCGG CCTGGCTCGC GCCTTCGGGG TGCCCCTGCG CACCTACACC 
CATGAGGTGG TGACACTGTG GTATCGCGCC CCCGAGATTC TCTTGGGCAG CAAGTTCTAT 
ACCACAGCTG TGGATATCTG GAGCATTGGT TGCATCTTTG CAGAGATGGT GACTCGAAAA 
GCCCTGTTTC CTGGTGACTC TGAGATTGAC CAGCTCTTTC GTATCTTTCG TATGCTGGGG 
ACACCCAGCG AAGACACATG GCCCGGGGTC ACCCAGCTGC CTGACTATAA GGGCAGCTTC 
CCTAAGTGGA CCAGGAAGGG ACTGGAAGAG ATTGTGCCCA ATCTGGAGCC AGAGGGCAGG 
GACCTGCTCA TGCAACTCCT GCAGTATGAC CCCAGCCAGC GGATCACAGC CAAGACTGCC 
CTGGCCCACC CGTACTTCTC ATCCCCTGAG CCCTCCCCAG CTGCCCGCCA GTATGTGCTG 
CAGCGATTCC GCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis.
Biotechnology letters(2018 Jan)
Zhang Y,Yang L,Ling C,Heng W

miR-125a-3p inhibits ER? transactivation and overrides tamoxifen resistance by targeting CDK3 in estrogen receptor-positive breast cancer.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Sep)
Zheng L,Meng X,Li X,Zhang Y,Li C,Xiang C,Xing Y,Xia Y,Xi T

HuR promotes breast cancer cell proliferation and survival via binding to CDK3 mRNA.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie91788-795(2017 Jul)
Zhang Z,Huang A,Zhang A,Zhou C

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect.
Experimental and molecular pathology102(2)181-190(2017 04)
Wang L,Xi Y,Sun C,Zhang F,Jiang H,He Q,Li D

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CDK3 cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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