Polr2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr2b
Entrez Gene ID	231329
Full Name	polymerase (RNA) II (DNA directed) polypeptide B
Synonyms	Pol2rb,Rpb140,Rpb2
General protein information	Preferred Names polymerase (RNA) II (DNA directed) polypeptide B Names DNA-directed RNA polymerase II subunit RPB2 DNA-directed RNA polymerase II 140 kDa polypeptide DNA-directed RNA polymerase II subunit B RNA polymerase II subunit 2 RNA polymerase II subunit B2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_153798.2	NP_722493.2	DNA-directed RNA polymerase II subunit RPB2

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLR2B	NP_000929.1
Rattus norvegicus (Norway rat)	Polr2b	NP_001099472.1
Gallus gallus (chicken)	POLR2B	NP_001006448.1
Drosophila melanogaster (fruit fly)	RpII140	NP_476706.1
Mus musculus (house mouse)	Polr2b	NP_722493.2
Danio rerio (zebrafish)	polr2b	NP_001019632.2
Schizosaccharomyces pombe (fission yeast)	rpb2	NP_593101.2
Magnaporthe oryzae (rice blast fungus)	MGG_04714	XP_003710821.1
Neurospora crassa	NCU05120	XP_957106.2
Arabidopsis thaliana (thale cress)	NRPB2	NP_193902.1
Canis lupus familiaris (dog)	POLR2B	XP_532382.3
Saccharomyces cerevisiae (baker's yeast)	RPB2	NP_014794.3
Kluyveromyces lactis	KLLA0B05577g	XP_451784.1
Eremothecium gossypii	AGOS_AFR404C	NP_985951.1
	POLR2B	XP_001083325.2
Pan troglodytes (chimpanzee)	POLR2B	XP_517275.2
Bos taurus (cattle)	POLR2B	NP_001092552.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003648	XP_313416.4
Caenorhabditis elegans (roundworm)	rpb-2	NP_498047.1
Oryza sativa (rice)	Os03g0646800	NP_001050770.1
Xenopus tropicalis (tropical clawed frog)	polr2b	XP_002937598.2

Gene Ontology
Bibliography

Related articles in PubMed

Reference genes in the developing murine brain and in differentiating embryonic stem cells.
Kraemer N, Neubert G, Issa L, Ninnemann O, Seiler AE, Kaindl AM
Neurological research34(7)664-8(2012 Sep)

A multiplicity of factors contributes to selective RNA polymerase III occupancy of a subset of RNA polymerase III genes in mouse liver.
Canella D, Bernasconi D, Gilardi F, LeMartelot G, Migliavacca E, Praz V, Cousin P, Delorenzi M, Hernandez N,
Genome research22(4)666-80(2012 Apr)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
Kannan S, Huang H, Seeger D, Audet A, Chen Y, Huang C, Gao H, Li S, Wu M
PloS one4(3)e4891(2009)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

For mouse embryonic stem cells with induced neural differentiation, all strategies used in parallel indicate RpII to be expressed in a stable manner.
Title: Reference genes in the developing murine brain and in differentiating embryonic stem cells.

NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters to permit strong transcriptional activation independently of histone modifications
Title: NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes.

The following Polr2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03065	NM_153798.2 Latest version!	Mus musculus polymerase (RNA) II (DNA directed) polypeptide B (Polr2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr2b ( NM_153798.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_722493.2 Mus musculus polymerase (RNA) II (DNA directed) polypeptide B (Polr2b), mRNA.

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CloneID	OMu03065
Clone ID Related Accession (Same CDS sequence)	NM_153798.2
Accession Version	NM_153798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK039766.1, CN690754.1, CF155991.1, CF553167.1, BQ177004.1 and AA501042.1. On Apr 18, 2009 this sequence version replaced NM_153798.1. ##Evidence-Data-START## Transcript exon combination :: BC016415.1, AK039766.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGACG CCGACGAGGA CATGCAATAT GATGAAGACG ACGATGAGAT CACTCCGGAT 
CTGTGGCAGG AAGCATGCTG GATCGTGATC AGTTCTTACT TTGATGAGAA AGGCTTGGTC 
AGACAACAGC TGGATTCCTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GATGCACCAC CGATTGACCT ACAGGCCGAA GCTCAGCACG CCAGTGGAGA AGTTGAAGAG 
CCGCCAAGAT ATTTACTGAA GTTTGAACAA ATTTACCTCT CCAAACCTAC TCATTGGGAA 
AGAGATGGTG CCCCTTCGCC AATGATGCCA AATGAAGCCA GGTTAAGAAA TCTCACGTAT 
TCTGCTCCGC TTTACGTTGA CATAACAAAG ACAGTCATTA AAGAAGGTGA AGAACAGCTG 
CAGACTCAGC ACCAGAAAAC GTTCATAGGG AAAATTCCAA TTATGCTGCG CTCCACCTAC 
TGCCTGTTAA ATGGCTTAAC AGATCGTGAC CTGTGTGAGC TGAATGAGTG TCCTTTGGAT 
CCCGGTGGCT ATTTTATTAT CAATGGATCA GAAAAGGTTC TGATAGCTCA AGAGAAAATG 
GCAACAAACA CAGTCTATGT GTTTGCCAAA AAGGATTCCA AGTATGCCTA TACAGGAGAA 
TGTCGCTCAT GCCTGGAGAA TTCTTCCCGA CCCACCAGTA CTATATGGGT TAGCATGCTG 
GCCAGAGGAG GACAGGGTGC AAAGAAGAGT GCTATTGGCC AGCGCATCGT GGCAACGTTA 
CCATACATCA AGCAAGAAGT TCCTATCATC ATTGTGTTCA GAGCACTAGG CTTTGTGTCT 
GATAGAGATA TTTTAGAACA CATCATTTAT GACTTCGAGG ATCCAGAGAT GATGGAAATG 
GTTAAACCAT CTCTCGATGA AGCGTTTGTC ATCCAAGAGC AGAATGTTGC CTTAAACTTC 
ATCGGTTCAA GAGGGGCAAA GCCTGGTGTT ACTAAGGAGA AAAGAATTAA ATATGCAAAA 
GAAGTCTTAC AGAAAGAAAT GCTCCCTCAC GTTGGTGTCA GTGATTTCTG TGAGACCAAA 
AAGGCCTATT TCTTGGGGTA TATGGTTCAT AGGTTGCTTC TAGCAGCCCT AGGCAGAAGA 
GAACTAGACG ACAGAGATCA CTATGGCAAC AAGAGATTGG ATCTTGCTGG GCCTCTGCTT 
GCATTCTTAT TCAGAGGCAT GTTTAAGAAT TTGCTGAAAG AAGTACGAAT CTATGCGCAG 
AAGTTTATTG ATCGCGGGAA GGATTTTAAC TTAGAGCTGG CAATCAAGAC AAGGATCATA 
TCTGATGGGC TAAAATACTC TTTAGCCACT GGGAACTGGG GTGACCAGAA GAAAGCTCAT 
CAAGCCAGAG CTGGAGTATC CCAGGTATTG AACCGCCTGA CGTTTGCATC TACTCTTTCT 
CACCTGCGTC GTCTAAACTC TCCTATTGGC AGAGATGGCA AGCTAGCGAA GCCCAGACAG 
TTGCATAATA CCTTGTGGGG AATGGTGTGT CCTGCTGAAA CTCCAGAGGG CCATGCTGTA 
GGGCTTGTGA AGAATTTAGC CCTGATGGCT TATATTTCAG TTGGATCTCA ACCCTCTCCA 
ATTTTGGAAT TTTTAGAAGA ATGGAGCATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATCCA TAAAGACCCC 
GAACAACTCA TGAACACGCT AAGGAAGTTA CGGCGGCAGA TGGACATCAT TGTATCCGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGCGG GAGATTCGGA TCTATACAGA TGCAGGCCGT 
ATTTGTAGAC CCCTTCTGAT TGTGGAAAAG CAGAAGCTGC TTTTGAAGAA GAGACACATT 
GACCAGTTGA AAGAGAGAGA ATATAACAAC TACAGTTGGC AGGATCTGGT AGCCAGTGGG 
GTAGTAGAGT ATATTGATAC CCTGGAAGAG GAGACCGTGA TGCTTGCAAT GACTCCAGAT 
GATTTGCAGG AGAAGGAAGT AGCTTATTGT TCTACCTACA CCCACTGTGA GATTCACCCA 
TCAATGATTC TTGGAGTCTG TGCATCTATT ATTCCTTTTC CTGATCATAA CCAGTCCCCT 
AGAAACACAT ACCAGTCTGC TATGGGCAAG CAGGCTATGG GAGTTTATAT CACCAACTTC 
CATGTGCGGA TGGACACCTT GGCCCATGTT CTCTACTATC CTCAGAAACC CCTTGTGACT 
ACACGGTCTA TGGAATATCT ACGATTCAGA GAGCTGCCAG CAGGAATCAA CTCAATTGTG 
GCCATTGCAT CCTACACGGG CTATAATCAA GAAGACTCTG TTATCATGAA TCGTTCAGCT 
GTGGATCGAG GCTTTTTCAG GTCTGTTTTC TACCGATCAT ACAAAGAACA AGAGTCTAAA 
AAGGGATTTG ATCAAGAAGA AGTGTTTGAG AAGCCTACAC GTGAGACATG CCAGGGAATG 
AGGCATGCCA TCTATGAAAA GTTGGACGAT GACGGTTTGA TAGCTCCGGG GGTCCGAGTC 
TCCGGAGATG ACGTTATTAT CGGCAAGACA GTCACCTTAC CCGAGAACGA GGATGAGCTG 
GAGAGCACCA ACAGGCGCTA CACCAAGAGG GACTGCAGCA CTTTCCTCAG AACTAGTGAG 
ACGGGCATCG TGGACCAGGT CATGGTCACT CTCAACCAGG AAGGATATAA ATTTTGTAAA 
ATAAGGGTGC GCTCTGTTAG AATTCCGCAG ATTGGAGACA AATTTGCTAG CCGACATGGT 
CAGAAGGGAA CTTGTGGTAT TCAGTATAGA CAAGAGGACA TGCCGTTCAC TTGCGAAGGC 
ATAACTCCTG ACATCATCAT AAACCCCCAC GCCATCCCCT CCCGGATGAC AATTGGTCAC 
TTGATCGAAT GTCTCCAAGG GAAGGTCTCT GCCAACAAGG GTGAAATTGG TGACGCTACT 
CCATTTAATG ATGCTGTCAA TGTGCAGAAG ATTTCTAATC TTTTGTCTGA CTATGGCTAC 
CATCTCAGAG GGAACGAGGT GCTCTACAAT GGATTCACTG GACGAAAAAT CACATCACAG 
ATTTTTATTG GCCCCACTTA CTACCAGCGT TTGAAGCATA TGGTGGATGA CAAAATTCAT 
TCTCGTGCTA GGGGACCCAT TCAGATTCTT AACAGACAGC CCATGGAGGG CAGATCTCGT 
GATGGTGGCC TGCGCTTCGG AGAAATGGAA CGAGACTGTC AAATTGCCCA CGGTGCAGCA 
CAGTTTCTAA GAGAAAGACT GTTTGAAGCA TCAGATCCGT ATCAGGTTCA TGTCTGTAAT 
CTTTGTGGAA TAATGGCAAT TGCTAACACG AGGACTCATA CATATGAGTG CAGGGGATGC 
CGCAATAAAA CCCAGATTTC CTTGGTGCGA ATGCCGTACG CGTGCAAGCT GTTGTTCCAG 
GAGCTCATGT CCATGAGCAT CGCACCACGA ATGATGAGTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722493.2
CDS	129..3653
Translation	MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYEKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	NM_153798.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (RNA) II (DNA directed) polypeptide B (Polr2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153798.2

ORF Insert Sequence:

ATGTATGACG CCGACGAGGA CATGCAATAT GATGAAGACG ACGATGAGAT CACTCCGGAT 
CTGTGGCAGG AAGCATGCTG GATCGTGATC AGTTCTTACT TTGATGAGAA AGGCTTGGTC 
AGACAACAGC TGGATTCCTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GATGCACCAC CGATTGACCT ACAGGCCGAA GCTCAGCACG CCAGTGGAGA AGTTGAAGAG 
CCGCCAAGAT ATTTACTGAA GTTTGAACAA ATTTACCTCT CCAAACCTAC TCATTGGGAA 
AGAGATGGTG CCCCTTCGCC AATGATGCCA AATGAAGCCA GGTTAAGAAA TCTCACGTAT 
TCTGCTCCGC TTTACGTTGA CATAACAAAG ACAGTCATTA AAGAAGGTGA AGAACAGCTG 
CAGACTCAGC ACCAGAAAAC GTTCATAGGG AAAATTCCAA TTATGCTGCG CTCCACCTAC 
TGCCTGTTAA ATGGCTTAAC AGATCGTGAC CTGTGTGAGC TGAATGAGTG TCCTTTGGAT 
CCCGGTGGCT ATTTTATTAT CAATGGATCA GAAAAGGTTC TGATAGCTCA AGAGAAAATG 
GCAACAAACA CAGTCTATGT GTTTGCCAAA AAGGATTCCA AGTATGCCTA TACAGGAGAA 
TGTCGCTCAT GCCTGGAGAA TTCTTCCCGA CCCACCAGTA CTATATGGGT TAGCATGCTG 
GCCAGAGGAG GACAGGGTGC AAAGAAGAGT GCTATTGGCC AGCGCATCGT GGCAACGTTA 
CCATACATCA AGCAAGAAGT TCCTATCATC ATTGTGTTCA GAGCACTAGG CTTTGTGTCT 
GATAGAGATA TTTTAGAACA CATCATTTAT GACTTCGAGG ATCCAGAGAT GATGGAAATG 
GTTAAACCAT CTCTCGATGA AGCGTTTGTC ATCCAAGAGC AGAATGTTGC CTTAAACTTC 
ATCGGTTCAA GAGGGGCAAA GCCTGGTGTT ACTAAGGAGA AAAGAATTAA ATATGCAAAA 
GAAGTCTTAC AGAAAGAAAT GCTCCCTCAC GTTGGTGTCA GTGATTTCTG TGAGACCAAA 
AAGGCCTATT TCTTGGGGTA TATGGTTCAT AGGTTGCTTC TAGCAGCCCT AGGCAGAAGA 
GAACTAGACG ACAGAGATCA CTATGGCAAC AAGAGATTGG ATCTTGCTGG GCCTCTGCTT 
GCATTCTTAT TCAGAGGCAT GTTTAAGAAT TTGCTGAAAG AAGTACGAAT CTATGCGCAG 
AAGTTTATTG ATCGCGGGAA GGATTTTAAC TTAGAGCTGG CAATCAAGAC AAGGATCATA 
TCTGATGGGC TAAAATACTC TTTAGCCACT GGGAACTGGG GTGACCAGAA GAAAGCTCAT 
CAAGCCAGAG CTGGAGTATC CCAGGTATTG AACCGCCTGA CGTTTGCATC TACTCTTTCT 
CACCTGCGTC GTCTAAACTC TCCTATTGGC AGAGATGGCA AGCTAGCGAA GCCCAGACAG 
TTGCATAATA CCTTGTGGGG AATGGTGTGT CCTGCTGAAA CTCCAGAGGG CCATGCTGTA 
GGGCTTGTGA AGAATTTAGC CCTGATGGCT TATATTTCAG TTGGATCTCA ACCCTCTCCA 
ATTTTGGAAT TTTTAGAAGA ATGGAGCATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATCCA TAAAGACCCC 
GAACAACTCA TGAACACGCT AAGGAAGTTA CGGCGGCAGA TGGACATCAT TGTATCCGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGCGG GAGATTCGGA TCTATACAGA TGCAGGCCGT 
ATTTGTAGAC CCCTTCTGAT TGTGGAAAAG CAGAAGCTGC TTTTGAAGAA GAGACACATT 
GACCAGTTGA AAGAGAGAGA ATATAACAAC TACAGTTGGC AGGATCTGGT AGCCAGTGGG 
GTAGTAGAGT ATATTGATAC CCTGGAAGAG GAGACCGTGA TGCTTGCAAT GACTCCAGAT 
GATTTGCAGG AGAAGGAAGT AGCTTATTGT TCTACCTACA CCCACTGTGA GATTCACCCA 
TCAATGATTC TTGGAGTCTG TGCATCTATT ATTCCTTTTC CTGATCATAA CCAGTCCCCT 
AGAAACACAT ACCAGTCTGC TATGGGCAAG CAGGCTATGG GAGTTTATAT CACCAACTTC 
CATGTGCGGA TGGACACCTT GGCCCATGTT CTCTACTATC CTCAGAAACC CCTTGTGACT 
ACACGGTCTA TGGAATATCT ACGATTCAGA GAGCTGCCAG CAGGAATCAA CTCAATTGTG 
GCCATTGCAT CCTACACGGG CTATAATCAA GAAGACTCTG TTATCATGAA TCGTTCAGCT 
GTGGATCGAG GCTTTTTCAG GTCTGTTTTC TACCGATCAT ACAAAGAACA AGAGTCTAAA 
AAGGGATTTG ATCAAGAAGA AGTGTTTGAG AAGCCTACAC GTGAGACATG CCAGGGAATG 
AGGCATGCCA TCTATGAAAA GTTGGACGAT GACGGTTTGA TAGCTCCGGG GGTCCGAGTC 
TCCGGAGATG ACGTTATTAT CGGCAAGACA GTCACCTTAC CCGAGAACGA GGATGAGCTG 
GAGAGCACCA ACAGGCGCTA CACCAAGAGG GACTGCAGCA CTTTCCTCAG AACTAGTGAG 
ACGGGCATCG TGGACCAGGT CATGGTCACT CTCAACCAGG AAGGATATAA ATTTTGTAAA 
ATAAGGGTGC GCTCTGTTAG AATTCCGCAG ATTGGAGACA AATTTGCTAG CCGACATGGT 
CAGAAGGGAA CTTGTGGTAT TCAGTATAGA CAAGAGGACA TGCCGTTCAC TTGCGAAGGC 
ATAACTCCTG ACATCATCAT AAACCCCCAC GCCATCCCCT CCCGGATGAC AATTGGTCAC 
TTGATCGAAT GTCTCCAAGG GAAGGTCTCT GCCAACAAGG GTGAAATTGG TGACGCTACT 
CCATTTAATG ATGCTGTCAA TGTGCAGAAG ATTTCTAATC TTTTGTCTGA CTATGGCTAC 
CATCTCAGAG GGAACGAGGT GCTCTACAAT GGATTCACTG GACGAAAAAT CACATCACAG 
ATTTTTATTG GCCCCACTTA CTACCAGCGT TTGAAGCATA TGGTGGATGA CAAAATTCAT 
TCTCGTGCTA GGGGACCCAT TCAGATTCTT AACAGACAGC CCATGGAGGG CAGATCTCGT 
GATGGTGGCC TGCGCTTCGG AGAAATGGAA CGAGACTGTC AAATTGCCCA CGGTGCAGCA 
CAGTTTCTAA GAGAAAGACT GTTTGAAGCA TCAGATCCGT ATCAGGTTCA TGTCTGTAAT 
CTTTGTGGAA TAATGGCAAT TGCTAACACG AGGACTCATA CATATGAGTG CAGGGGATGC 
CGCAATAAAA CCCAGATTTC CTTGGTGCGA ATGCCGTACG CGTGCAAGCT GTTGTTCCAG 
GAGCTCATGT CCATGAGCAT CGCACCACGA ATGATGAGTG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reference genes in the developing murine brain and in differentiating embryonic stem cells.
Neurological research34(7)664-8(2012 Sep)
Kraemer N,Neubert G,Issa L,Ninnemann O,Seiler AE,Kaindl AM

A multiplicity of factors contributes to selective RNA polymerase III occupancy of a subset of RNA polymerase III genes in mouse liver.
Genome research22(4)666-80(2012 Apr)
Canella D,Bernasconi D,Gilardi F,LeMartelot G,Migliavacca E,Praz V,Cousin P,Delorenzi M,Hernandez N,

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Alveolar epithelial type II cells activate alveolar macrophages and mitigate P. Aeruginosa infection.
PloS one4(3)e4891(2009)
Kannan S,Huang H,Seeger D,Audet A,Chen Y,Huang C,Gao H,Li S,Wu M

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

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Polr2b cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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