Nkx2-2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nkx2-2
Entrez Gene ID	366214
Full Name	NK2 homeobox 2
General protein information	Preferred Names NK2 homeobox 2 Names homeobox protein Nkx-2.2 NK2 transcription factor related, locus 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591951.1	XP_017447440.1	homeobox protein Nkx-2.2 isoform X1
NM_001191904.1	NP_001178833.1	homeobox protein Nkx-2.2

Pathways from BioSystems

Homologies

	NKX2-2	XP_001094853.1
Pan troglodytes (chimpanzee)	NKX2-2	XP_525279.2
Rattus norvegicus (Norway rat)	Nkx2-2	NP_001178833.1
Homo sapiens (human)	NKX2-2	NP_002500.1
Bos taurus (cattle)	NKX2-2	NP_001179638.1
Mus musculus (house mouse)	Nkx2-2	NP_035049.1
Gallus gallus (chicken)	NKX2-2	NP_001264647.1
Canis lupus familiaris (dog)	NKX2-2	XP_542867.2
Danio rerio (zebrafish)	nkx2.2a	NP_571497.1
Xenopus tropicalis (tropical clawed frog)	nkx2-2	XP_002939477.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-23a promotes myelination in the central nervous system.
Lin ST, Huang Y, Zhang L, Heng MY, Pt?cek LJ, Fu YH
Proceedings of the National Academy of Sciences of the United States of America110(43)17468-73(2013 Oct)

Ectopic transgenic expression of NKX2.2 induces differentiation of adult pancreatic progenitors and mediates islet regeneration.
Chen S, Shimoda M, Chen J, Matsumoto S, Grayburn PA
Cell cycle (Georgetown, Tex.)11(8)1544-53(2012 Apr)

Identification of Juxtanodin promoter and its transcriptional regulation during the ATRA-induced differentiation of C6 cells.
Wang T, Jia L, Yang G, Ji S, Yao L, Zhang B
Molecular and cellular biochemistry350(1-2)177-83(2011 Apr)

Cooperative transcriptional regulation of the essential pancreatic islet gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3.
Anderson KR, Torres CA, Solomon K, Becker TC, Newgard CB, Wright CV, Hagman J, Sussel L
The Journal of biological chemistry284(45)31236-48(2009 Nov)

Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain.
Prakash N, Puelles E, Freude K, Tr?mbach D, Omodei D, Di Salvio M, Sussel L, Ericson J, Sander M, Simeone A, Wurst W
Development (Cambridge, England)136(15)2545-55(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nkx2.2 targeted to the pancreas by UTMD induces pancreatic progenitor cell proliferation and differentiation with subsequent islet regeneration and cure of STZ-induced diabetes for three months.
Title: Ectopic transgenic expression of NKX2.2 induces differentiation of adult pancreatic progenitors and mediates islet regeneration.

Nkx2.2 is one of the essential transcription factors delivering the specificity of JN promoter in oligodendrocytes.
Title: Identification of Juxtanodin promoter and its transcriptional regulation during the ATRA-induced differentiation of C6 cells.

These results suggest that environmental factors such as cytokines may affect Nkx2.2 expression or oligodendrocyte precursor cell differentiation in the contusion-induced spinal cord injury model.
Title: Nkx2.2 expression in differentiation of oligodendrocyte precursor cells and inhibitory factors for differentiation of oligodendrocytes after traumatic spinal cord injury.

prevents the precocious expression of myelin basic protein in immature oligodendrocytes
Title: Stage-specific expression of myelin basic protein in oligodendrocytes involves Nkx2.2-mediated repression that is relieved by the Sp1 transcription factor.

At 7 dpi, a significant up-regulation of Nkx2.2 by OPCs within the lesion was observed, 90% of which coexpressed NG2 and virtually all of which coexpressed the bHLH transcription factor Olig2.
Title: Endogenous Nkx2.2+/Olig2+ oligodendrocyte precursor cells fail to remyelinate the demyelinated adult rat spinal cord in the absence of astrocytes.

The following Nkx2-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkx2-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07989	NM_001191904.1 Latest version!	Rattus norvegicus NK2 homeobox 2 (Nkx2-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa07989	XM_017591951.1 Latest version!	Rattus norvegicus NK2 homeobox 2 (Nkx2-2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nkx2-2 ( NM_001191904.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178833.1 Rattus norvegicus NK2 homeobox 2 (Nkx2-2), mRNA.

Price & Availability↑

CloneID	ORa07989
Clone ID Related Accession (Same CDS sequence)	XM_017591951.1 , NM_001191904.1
Accession Version	NM_001191904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	homeobox protein Nkx-2.2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07054109.1. On Jul 17, 2010 this sequence version replaced XM_345446.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689605, SAMN02642610 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCTGA CCAACACAAA GACGGGGTTT TCAGTCAAGG ACATCTTGGA CCTTCCGGAC 
ACCAACGATG AAGAAGGCTC GGTGGCCGAA GGGCCGGAGG AGGAGAACGA AGGGCCGGAG 
CCGGCCAAGA GGGCCGGGCC GCTAGGGCAG AGCGCCTTGG ATGCTGTGCA GAGCCTGCCC 
CTTAAGAGTC CTTTCTACGA CAGCAGCGAC AACCCCTACA CTCGCTGGCT GGCCAGCACC 
GAGGGCCTCC AATACTCCCT GCACGGACTG GCGGCGAGCG CTCCCCCCCA AGACTCGAGC 
TCCAAGTCCC CAGAGCCCTC GGCTGACGAG TCACCGGACA ATGACAAGGA GACCCAGGGC 
GGCGGGGGGG ACGCAGGCAA GAAGCGGAAG CGCCGAGTGC TCTTCTCCAA AGCGCAGACC 
TACGAGCTGG AGCGGCGCTT CCGGCAGCAG CGGTACCTGT CGGCGCCCGA GCGCGAGCAC 
CTGGCCAGCC TCATCCGTCT CACGCCCACG CAGGTCAAGA TCTGGTTCCA AAACCATCGC 
TACAAGATGA AACGTGCCCG GGCTGAGAAA GGTATGGAGG TGACGCCTCT ACCCTCGCCG 
CGCCGTGTGG CAGTGCCGGT CTTGGTCAGG GACGGCAAAC CGTGCCACGC GCTCAAAGCC 
CAGGACCTGG CAGCTGCCAC CTTCCAGGCA GGCATCCCCT TTTCCGCCTA CAGCGCGCAG 
TCGCTGCAGC ACATGCAATA CAACGCCCAG TACAGCTCGG CCAGCACCCC CCAGTACCCG 
ACAGCACACC CCCTGGTCCA GGCCCAGCAG TGGACTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178833.1
CDS	297..1118
Translation	MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQSALDAVQSLPLKSPFYDSSDNPYTRWLASTEGLQYSLHGLAASAPPQDSSSKSPEPSADESPDNDKETQGGGGDAGKKRKRRVLFSKAQTYELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSPRRVAVPVLVRDGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHMQYNAQYSSASTPQYPTAHPLVQAQQWTW

Target ORF information:

RefSeq Version	NM_001191904.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NK2 homeobox 2 (Nkx2-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191904.1

ORF Insert Sequence:

ATGTCGCTGA CCAACACAAA GACGGGGTTT TCAGTCAAGG ACATCTTGGA CCTTCCGGAC 
ACCAACGATG AAGAAGGCTC GGTGGCCGAA GGGCCGGAGG AGGAGAACGA AGGGCCGGAG 
CCGGCCAAGA GGGCCGGGCC GCTAGGGCAG AGCGCCTTGG ATGCTGTGCA GAGCCTGCCC 
CTTAAGAGTC CTTTCTACGA CAGCAGCGAC AACCCCTACA CTCGCTGGCT GGCCAGCACC 
GAGGGCCTCC AATACTCCCT GCACGGACTG GCGGCGAGCG CTCCCCCCCA AGACTCGAGC 
TCCAAGTCCC CAGAGCCCTC GGCTGACGAG TCACCGGACA ATGACAAGGA GACCCAGGGC 
GGCGGGGGGG ACGCAGGCAA GAAGCGGAAG CGCCGAGTGC TCTTCTCCAA AGCGCAGACC 
TACGAGCTGG AGCGGCGCTT CCGGCAGCAG CGGTACCTGT CGGCGCCCGA GCGCGAGCAC 
CTGGCCAGCC TCATCCGTCT CACGCCCACG CAGGTCAAGA TCTGGTTCCA AAACCATCGC 
TACAAGATGA AACGTGCCCG GGCTGAGAAA GGTATGGAGG TGACGCCTCT ACCCTCGCCG 
CGCCGTGTGG CAGTGCCGGT CTTGGTCAGG GACGGCAAAC CGTGCCACGC GCTCAAAGCC 
CAGGACCTGG CAGCTGCCAC CTTCCAGGCA GGCATCCCCT TTTCCGCCTA CAGCGCGCAG 
TCGCTGCAGC ACATGCAATA CAACGCCCAG TACAGCTCGG CCAGCACCCC CCAGTACCCG 
ACAGCACACC CCCTGGTCCA GGCCCAGCAG TGGACTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nkx2-2 ( XM_017591951.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447440.1 Rattus norvegicus NK2 homeobox 2 (Nkx2-2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07989
Clone ID Related Accession (Same CDS sequence)	XM_017591951.1 , NM_001191904.1
Accession Version	XM_017591951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	homeobox protein Nkx-2.2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA CCAACACAAA GACGGGGTTT TCAGTCAAGG ACATCTTGGA CCTTCCGGAC 
ACCAACGATG AAGAAGGCTC GGTGGCCGAA GGGCCGGAGG AGGAGAACGA AGGGCCGGAG 
CCGGCCAAGA GGGCCGGGCC GCTAGGGCAG AGCGCCTTGG ATGCTGTGCA GAGCCTGCCC 
CTTAAGAGTC CTTTCTACGA CAGCAGCGAC AACCCCTACA CTCGCTGGCT GGCCAGCACC 
GAGGGCCTCC AATACTCCCT GCACGGACTG GCGGCGAGCG CTCCCCCCCA AGACTCGAGC 
TCCAAGTCCC CAGAGCCCTC GGCTGACGAG TCACCGGACA ATGACAAGGA GACCCAGGGC 
GGCGGGGGGG ACGCAGGCAA GAAGCGGAAG CGCCGAGTGC TCTTCTCCAA AGCGCAGACC 
TACGAGCTGG AGCGGCGCTT CCGGCAGCAG CGGTACCTGT CGGCGCCCGA GCGCGAGCAC 
CTGGCCAGCC TCATCCGTCT CACGCCCACG CAGGTCAAGA TCTGGTTCCA AAACCATCGC 
TACAAGATGA AACGTGCCCG GGCTGAGAAA GGTATGGAGG TGACGCCTCT ACCCTCGCCG 
CGCCGTGTGG CAGTGCCGGT CTTGGTCAGG GACGGCAAAC CGTGCCACGC GCTCAAAGCC 
CAGGACCTGG CAGCTGCCAC CTTCCAGGCA GGCATCCCCT TTTCCGCCTA CAGCGCGCAG 
TCGCTGCAGC ACATGCAATA CAACGCCCAG TACAGCTCGG CCAGCACCCC CCAGTACCCG 
ACAGCACACC CCCTGGTCCA GGCCCAGCAG TGGACTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447440.1
CDS	725..1546
Translation	MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQSALDAVQSLPLKSPFYDSSDNPYTRWLASTEGLQYSLHGLAASAPPQDSSSKSPEPSADESPDNDKETQGGGGDAGKKRKRRVLFSKAQTYELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSPRRVAVPVLVRDGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHMQYNAQYSSASTPQYPTAHPLVQAQQWTW

Target ORF information:

RefSeq Version	XM_017591951.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NK2 homeobox 2 (Nkx2-2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591951.1

ORF Insert Sequence:

ATGTCGCTGA CCAACACAAA GACGGGGTTT TCAGTCAAGG ACATCTTGGA CCTTCCGGAC 
ACCAACGATG AAGAAGGCTC GGTGGCCGAA GGGCCGGAGG AGGAGAACGA AGGGCCGGAG 
CCGGCCAAGA GGGCCGGGCC GCTAGGGCAG AGCGCCTTGG ATGCTGTGCA GAGCCTGCCC 
CTTAAGAGTC CTTTCTACGA CAGCAGCGAC AACCCCTACA CTCGCTGGCT GGCCAGCACC 
GAGGGCCTCC AATACTCCCT GCACGGACTG GCGGCGAGCG CTCCCCCCCA AGACTCGAGC 
TCCAAGTCCC CAGAGCCCTC GGCTGACGAG TCACCGGACA ATGACAAGGA GACCCAGGGC 
GGCGGGGGGG ACGCAGGCAA GAAGCGGAAG CGCCGAGTGC TCTTCTCCAA AGCGCAGACC 
TACGAGCTGG AGCGGCGCTT CCGGCAGCAG CGGTACCTGT CGGCGCCCGA GCGCGAGCAC 
CTGGCCAGCC TCATCCGTCT CACGCCCACG CAGGTCAAGA TCTGGTTCCA AAACCATCGC 
TACAAGATGA AACGTGCCCG GGCTGAGAAA GGTATGGAGG TGACGCCTCT ACCCTCGCCG 
CGCCGTGTGG CAGTGCCGGT CTTGGTCAGG GACGGCAAAC CGTGCCACGC GCTCAAAGCC 
CAGGACCTGG CAGCTGCCAC CTTCCAGGCA GGCATCCCCT TTTCCGCCTA CAGCGCGCAG 
TCGCTGCAGC ACATGCAATA CAACGCCCAG TACAGCTCGG CCAGCACCCC CCAGTACCCG 
ACAGCACACC CCCTGGTCCA GGCCCAGCAG TGGACTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-23a promotes myelination in the central nervous system.
Proceedings of the National Academy of Sciences of the United States of America110(43)17468-73(2013 Oct)
Lin ST,Huang Y,Zhang L,Heng MY,Pt?cek LJ,Fu YH

Ectopic transgenic expression of NKX2.2 induces differentiation of adult pancreatic progenitors and mediates islet regeneration.
Cell cycle (Georgetown, Tex.)11(8)1544-53(2012 Apr)
Chen S,Shimoda M,Chen J,Matsumoto S,Grayburn PA

Identification of Juxtanodin promoter and its transcriptional regulation during the ATRA-induced differentiation of C6 cells.
Molecular and cellular biochemistry350(1-2)177-83(2011 Apr)
Wang T,Jia L,Yang G,Ji S,Yao L,Zhang B

Cooperative transcriptional regulation of the essential pancreatic islet gene NeuroD1 (beta2) by Nkx2.2 and neurogenin 3.
The Journal of biological chemistry284(45)31236-48(2009 Nov)
Anderson KR,Torres CA,Solomon K,Becker TC,Newgard CB,Wright CV,Hagman J,Sussel L

Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain.
Development (Cambridge, England)136(15)2545-55(2009 Aug)
Prakash N,Puelles E,Freude K,Tr?mbach D,Omodei D,Di Salvio M,Sussel L,Ericson J,Sander M,Simeone A,Wurst W

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Nkx2-2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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