Gug gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_206303.2	NP_996025.1	grunge, isoform D
NM_145106.1	NP_659574.1	grunge, isoform A
NM_176300.1	NP_788478.1	grunge, isoform C
NM_001259742.1	NP_001246671.1	grunge, isoform F
NM_001259743.1	NP_001246672.1	grunge, isoform G
NM_001274651.1	NP_001261580.1	grunge, isoform I
NM_001274652.1	NP_001261581.1	grunge, isoform J
NM_001259741.1	NP_001246670.1	grunge, isoform E
NM_079249.3	NP_523973.2	grunge, isoform H

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19921	NM_001259743.1 Latest version!	Drosophila melanogaster grunge, transcript variant G (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa19431	NM_079249.3 Latest version!	Drosophila melanogaster grunge, transcript variant H (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19432	NM_206303.2 Latest version!	Drosophila melanogaster grunge, transcript variant D (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19432	NM_001259741.1 Latest version!	Drosophila melanogaster grunge, transcript variant E (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19433	NM_176300.1 Latest version!	Drosophila melanogaster grunge, transcript variant C (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19434	NM_145106.1 Latest version!	Drosophila melanogaster grunge, transcript variant A (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19918	NM_001274652.1 Latest version!	Drosophila melanogaster grunge, transcript variant J (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa19919	NM_001259742.1 Latest version!	Drosophila melanogaster grunge, transcript variant F (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa19920	NM_001274651.1 Latest version!	Drosophila melanogaster grunge, transcript variant I (Gug), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

Gug ( NM_001259743.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246672.1 Drosophila melanogaster grunge, transcript variant G (Gug), mRNA.

Price & Availability↑

CloneID	OFa19921
Clone ID Related Accession (Same CDS sequence)	NM_001259743.1
Accession Version	NM_001259743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAAC AACAACAGCA GCAGCAACAA CAGCAAGCAG CGGCCGCGCA ACAGGCGGCA 
CAGCAAGCCA AGATGAAGGC TGAGCTGAAG CCACCGTATG CGGATACGCC GGCACTGCGT 
CAACTGTCCG AGTACGCTCG TCCCCACGTC GCCTTCAGTC CTGTTGAACA GATGGTGCCA 
TATCATCATC CAATGGGCCC CATGTACAGA GAGAGGGAAC TGGAGGAGAT TAAAAACGCA 
CAAGCTGCTG CGGCGAGTCA ATCCCGACTA GATCCGCACT GGATGGAATA CTATCGACGC 
GGCATCCACC CCTCGCAGTT CCCGCTGTAT GCGAATCCGG CGATATCGCA GATGGAGAGG 
GAGCGTCTGG GAATTCCACC TCCGCACCAT GTGGGGTTGG ACCCGGGCGA GCACATGGTG 
CGTATGATAC GATTGACGAG AGAATATCAT GCACACTCTC ATACTCATTT ACATTTGCCT 
TTGCATCCAC AGCCGCAACC ACCGGAGGCC GGTTTCCAAC TGCCACCGAA TGTTGGCCAG 
TATCCGCGGC CAAATATGCT TATACCTAGG GAGCCGCACT CGGATGTCCT GCTGCGCATG 
TCCTATGCCG ACCAACTACA GGCCGCCGAG TTTCAGCGAC AGTCCCTGCA TGATCAGTAC 
TTTAGACAAC GGCCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246672.1
CDS	606..6626
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFSPVEQMVPYHHPMGPMYRERELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMVRMIRLTREYHAHSHTHLHLPLHPQPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_001259743.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant G (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259743.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAAC AACAACAGCA GCAGCAACAA CAGCAAGCAG CGGCCGCGCA ACAGGCGGCA 
CAGCAAGCCA AGATGAAGGC TGAGCTGAAG CCACCGTATG CGGATACGCC GGCACTGCGT 
CAACTGTCCG AGTACGCTCG TCCCCACGTC GCCTTCAGTC CTGTTGAACA GATGGTGCCA 
TATCATCATC CAATGGGCCC CATGTACAGA GAGAGGGAAC TGGAGGAGAT TAAAAACGCA 
CAAGCTGCTG CGGCGAGTCA ATCCCGACTA GATCCGCACT GGATGGAATA CTATCGACGC 
GGCATCCACC CCTCGCAGTT CCCGCTGTAT GCGAATCCGG CGATATCGCA GATGGAGAGG 
GAGCGTCTGG GAATTCCACC TCCGCACCAT GTGGGGTTGG ACCCGGGCGA GCACATGGTG 
CGTATGATAC GATTGACGAG AGAATATCAT GCACACTCTC ATACTCATTT ACATTTGCCT 
TTGCATCCAC AGCCGCAACC ACCGGAGGCC GGTTTCCAAC TGCCACCGAA TGTTGGCCAG 
TATCCGCGGC CAAATATGCT TATACCTAGG GAGCCGCACT CGGATGTCCT GCTGCGCATG 
TCCTATGCCG ACCAACTACA GGCCGCCGAG TTTCAGCGAC AGTCCCTGCA TGATCAGTAC 
TTTAGACAAC GGCCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_079249.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523973.2 Drosophila melanogaster grunge, transcript variant H (Gug), mRNA.

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CloneID	OFa19431
Clone ID Related Accession (Same CDS sequence)	NM_079249.3
Accession Version	NM_079249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079249.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
GTGCGTATGC CGCAACCACC GGAGGCCGGT TTCCAACTGC CACCGAATGT TGGCCAGTAT 
CCGCGGCCAA ATATGCTTAT ACCTAGGGAG CCGCACTCGG ATGTCCTGCT GCGCATGTCC 
TATGCCGACC AACTACAGGC CGCCGAGTTT CAGCGACAGT CCCTGCATGA TCAGTACTTT 
AGACAACGGC CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523973.2
CDS	960..6917
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFSPVEQMVPYHHPMGPMYRERELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMVRMPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_079249.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant H (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079249.3

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
GTGCGTATGC CGCAACCACC GGAGGCCGGT TTCCAACTGC CACCGAATGT TGGCCAGTAT 
CCGCGGCCAA ATATGCTTAT ACCTAGGGAG CCGCACTCGG ATGTCCTGCT GCGCATGTCC 
TATGCCGACC AACTACAGGC CGCCGAGTTT CAGCGACAGT CCCTGCATGA TCAGTACTTT 
AGACAACGGC CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_206303.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996025.1 Drosophila melanogaster grunge, transcript variant D (Gug), mRNA.

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CloneID	OFa19432
Clone ID Related Accession (Same CDS sequence)	NM_206303.2 , NM_001259741.1
Accession Version	NM_206303.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_206303.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
CCGCAACCAC CGGAGGCCGG TTTCCAACTG CCACCGAATG TTGGCCAGTA TCCGCGGCCA 
AATATGCTTA TACCTAGGGA GCCGCACTCG GATGTCCTGC TGCGCATGTC CTATGCCGAC 
CAACTACAGT ATTTACAGGC CGCCGAGTTT CAGCGACAGT CCCTGCATGA TCAGTACTTT 
AGACAACGGC CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996025.1
CDS	960..6917
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFSPVEQMVPYHHPMGPMYRERELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQYLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_206303.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant D (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206303.2

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
CCGCAACCAC CGGAGGCCGG TTTCCAACTG CCACCGAATG TTGGCCAGTA TCCGCGGCCA 
AATATGCTTA TACCTAGGGA GCCGCACTCG GATGTCCTGC TGCGCATGTC CTATGCCGAC 
CAACTACAGT ATTTACAGGC CGCCGAGTTT CAGCGACAGT CCCTGCATGA TCAGTACTTT 
AGACAACGGC CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_001259741.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246670.1 Drosophila melanogaster grunge, transcript variant E (Gug), mRNA.

Price & Availability↑

CloneID	OFa19432
Clone ID Related Accession (Same CDS sequence)	NM_206303.2 , NM_001259741.1
Accession Version	NM_001259741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
CCGCAACCAC CGGAGGCCGG TTTCCAACTG CCACCGAATG TTGGCCAGTA TCCGCGGCCA 
AATATGCTTA TACCTAGGGA GCCGCACTCG GATGTCCTGC TGCGCATGTC CTATGCCGAC 
CAACTACAGT ATTTACAGGC CGCCGAGTTT CAGCGACAGT CCCTGCATGA TCAGTACTTT 
AGACAACGGC CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246670.1
CDS	606..6563
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFSPVEQMVPYHHPMGPMYRERELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQYLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_001259741.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant E (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259741.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
CCGCAACCAC CGGAGGCCGG TTTCCAACTG CCACCGAATG TTGGCCAGTA TCCGCGGCCA 
AATATGCTTA TACCTAGGGA GCCGCACTCG GATGTCCTGC TGCGCATGTC CTATGCCGAC 
CAACTACAGT ATTTACAGGC CGCCGAGTTT CAGCGACAGT CCCTGCATGA TCAGTACTTT 
AGACAACGGC CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_176300.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788478.1 Drosophila melanogaster grunge, transcript variant C (Gug), mRNA.

Price & Availability↑

CloneID	OFa19433
Clone ID Related Accession (Same CDS sequence)	NM_176300.1
Accession Version	NM_176300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
GTGCGTATGC CGCAACCACC GGAGGCCGGT TTCCAACTGC CACCGAATGT TGGCCAGTAT 
CCGCGGCCAA ATATGCTTAT ACCTAGGGAG CCGCACTCGG ATGTCCTGCT GCGCATGTCC 
TATGCCGACC AACTACAGTA TTTACAGGCC GCCGAGTTTC AGCGACAGTC CCTGCATGAT 
CAGTACTTTA GACAACGGCC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788478.1
CDS	464..6430
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFSPVEQMVPYHHPMGPMYRERELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMVRMPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQYLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_176300.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant C (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176300.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
GTGCGTATGC CGCAACCACC GGAGGCCGGT TTCCAACTGC CACCGAATGT TGGCCAGTAT 
CCGCGGCCAA ATATGCTTAT ACCTAGGGAG CCGCACTCGG ATGTCCTGCT GCGCATGTCC 
TATGCCGACC AACTACAGTA TTTACAGGCC GCCGAGTTTC AGCGACAGTC CCTGCATGAT 
CAGTACTTTA GACAACGGCC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_145106.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_659574.1 Drosophila melanogaster grunge, transcript variant A (Gug), mRNA.

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CloneID	OFa19434
Clone ID Related Accession (Same CDS sequence)	NM_145106.1
Accession Version	NM_145106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GGGAACTGGA GGAGATTAAA 
AACGCACAAG CTGCTGCGGC GAGTCAATCC CGACTAGATC CGCACTGGAT GGAATACTAT 
CGACGCGGCA TCCACCCCTC GCAGTTCCCG CTGTATGCGA ATCCGGCGAT ATCGCAGATG 
GAGAGGGAGC GTCTGGGAAT TCCACCTCCG CACCATGTGG GGTTGGACCC GGGCGAGCAC 
ATGGTGCGTA TGCCGCAACC ACCGGAGGCC GGTTTCCAAC TGCCACCGAA TGTTGGCCAG 
TATCCGCGGC CAAATATGCT TATACCTAGG GAGCCGCACT CGGATGTCCT GCTGCGCATG 
TCCTATGCCG ACCAACTACA GGCCGCCGAG TTTCAGCGAC AGTCCCTGCA TGATCAGTAC 
TTTAGACAAC GGCCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659574.1
CDS	464..6364
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFRELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMVRMPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_145106.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant A (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145106.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GGGAACTGGA GGAGATTAAA 
AACGCACAAG CTGCTGCGGC GAGTCAATCC CGACTAGATC CGCACTGGAT GGAATACTAT 
CGACGCGGCA TCCACCCCTC GCAGTTCCCG CTGTATGCGA ATCCGGCGAT ATCGCAGATG 
GAGAGGGAGC GTCTGGGAAT TCCACCTCCG CACCATGTGG GGTTGGACCC GGGCGAGCAC 
ATGGTGCGTA TGCCGCAACC ACCGGAGGCC GGTTTCCAAC TGCCACCGAA TGTTGGCCAG 
TATCCGCGGC CAAATATGCT TATACCTAGG GAGCCGCACT CGGATGTCCT GCTGCGCATG 
TCCTATGCCG ACCAACTACA GGCCGCCGAG TTTCAGCGAC AGTCCCTGCA TGATCAGTAC 
TTTAGACAAC GGCCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_001274652.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261581.1 Drosophila melanogaster grunge, transcript variant J (Gug), mRNA.

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CloneID	OFa19918
Clone ID Related Accession (Same CDS sequence)	NM_001274652.1
Accession Version	NM_001274652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
ATACGATTGA CGAGAGAATA TCATGCACAC TCTCATACTC ATTTACATTT GCCTTTGCAT 
CCACAGCCGC AACCACCGGA GGCCGGTTTC CAACTGCCAC CGAATGTTGG CCAGTATCCG 
CGGCCAAATA TGCTTATACC TAGGGAGCCG CACTCGGATG TCCTGCTGCG CATGTCCTAT 
GCCGACCAAC TACAGTATTT ACAGGCCGCC GAGTTTCAGC GACAGTCCCT GCATGATCAG 
TACTTTAGGA ATCAGCTGCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261581.1
CDS	960..6983
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFSPVEQMVPYHHPMGPMYRERELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMIRLTREYHAHSHTHLHLPLHPQPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQYLQAAEFQRQSLHDQYFRNQLR

Target ORF information:

RefSeq Version	NM_001274652.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant J (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274652.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GTCCTGTTGA ACAGATGGTG 
CCATATCATC ATCCAATGGG CCCCATGTAC AGAGAGAGGG AACTGGAGGA GATTAAAAAC 
GCACAAGCTG CTGCGGCGAG TCAATCCCGA CTAGATCCGC ACTGGATGGA ATACTATCGA 
CGCGGCATCC ACCCCTCGCA GTTCCCGCTG TATGCGAATC CGGCGATATC GCAGATGGAG 
AGGGAGCGTC TGGGAATTCC ACCTCCGCAC CATGTGGGGT TGGACCCGGG CGAGCACATG 
ATACGATTGA CGAGAGAATA TCATGCACAC TCTCATACTC ATTTACATTT GCCTTTGCAT 
CCACAGCCGC AACCACCGGA GGCCGGTTTC CAACTGCCAC CGAATGTTGG CCAGTATCCG 
CGGCCAAATA TGCTTATACC TAGGGAGCCG CACTCGGATG TCCTGCTGCG CATGTCCTAT 
GCCGACCAAC TACAGTATTT ACAGGCCGCC GAGTTTCAGC GACAGTCCCT GCATGATCAG 
TACTTTAGGA ATCAGCTGCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_001259742.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246671.1 Drosophila melanogaster grunge, transcript variant F (Gug), mRNA.

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CloneID	OFa19919
Clone ID Related Accession (Same CDS sequence)	NM_001259742.1
Accession Version	NM_001259742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GGGAACTGGA GGAGATTAAA 
AACGCACAAG CTGCTGCGGC GAGTCAATCC CGACTAGATC CGCACTGGAT GGAATACTAT 
CGACGCGGCA TCCACCCCTC GCAGTTCCCG CTGTATGCGA ATCCGGCGAT ATCGCAGATG 
GAGAGGGAGC GTCTGGGAAT TCCACCTCCG CACCATGTGG GGTTGGACCC GGGCGAGCAC 
ATGATACGAT TGACGAGAGA ATATCATGCA CACTCTCATA CTCATTTACA TTTGCCTTTG 
CATCCACAGC CGCAACCACC GGAGGCCGGT TTCCAACTGC CACCGAATGT TGGCCAGTAT 
CCGCGGCCAA ATATGCTTAT ACCTAGGGAG CCGCACTCGG ATGTCCTGCT GCGCATGTCC 
TATGCCGACC AACTACAGTA TTTACAGGCC GCCGAGTTTC AGCGACAGTC CCTGCATGAT 
CAGTACTTTA GACAACGGCC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246671.1
CDS	960..6926
Translation	MAASTQGEIRVGPGHQVNDVYAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFRELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMIRLTREYHAHSHTHLHLPLHPQPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQYLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_001259742.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant F (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259742.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGT AAACGATGTC 
TATGCAAAAC TGCCCGATTA TAATCCAATC TCAAGCTTCC CCATCGACAA GGAAACCGAT 
GAACGTGAAC TAGAGGAATC AAGATGGAGT CCAGGCGTTG TGGCCGATGG CGACTTGTTA 
ATGTTCTTGC GTGCGGCTCG ATCCATGGCT GCATTTCAAG GAATGTGTGA TGGTGGTTTA 
GAAGACGGTT GTTTGGCTGC TAGTCGCGAC GACACTACAA TAAACGCACT CGACGTGCTC 
CACGATTCTG GCTACGATCC AGGCAAAGCT CTACAAGCGC TCGTAAAGTG CCCCGTTTCG 
AAGGGCATCG ACAAGAAGTG GACCGAGGAC GAAACAAAGA AATTCATCAA GGGTCTGCGT 
CAGTTTGGGA AGAACTTCTT CCGCATCCAT AAGGACTTGC TGCCGCACAA GGACACGCCG 
GAGCTGGTCG AGTTCTACTA TCTGTGGAAG AAGACGCCCG GCGCGAACAA TAATCGGCCA 
CACAGGCGAC GCCGCCAAAG CGCCCTGCGA CGCAACCGTG TCACGCGGGC CAACAACAGC 
AACAGCAACA CTCCTCCGAA GAAGGAGGAC ACTCCAGAAC CACAAACTGC GACGACGGCG 
ACGGCGGCGG CAACCGCGGC GTCCGAGACG GCGAGTCGCT CCTCGCCCGC TGTCTCCAAG 
GAGGAGAACA GCTCGCTCAC CGAGGACGAC GCCAGCGAGT GCGACAGTGA TTCGAGTCTG 
ACCCACAAAA GGGATGAATC ACCCTCAAGG ATGAGGACGC GTAACAAGCA ACAGAACAAC 
AACAGCAGCA CCAGCAGCGG TAACAACACG GCCGGCAACG GTGGCGGTAA CGCCACATCC 
ATAAGCAGCG GATCAACCGG CGGCGGTGCC GCTGGCGGCA ATAGTTCGTC TAAGGATCAA 
TCAGCCAACG CCGTGGCTAA TGGCAAGCGA CCCAAGAGGG GCTCCGAAAC ACCGGACGTT 
TCCGGCGGAG CCTCGGTCGA TAGTCCCAAG ACACCGACGA AGGCTGTGGC CGAGAGTTCA 
GCCAATAAGC GCAAGGGTGG CAAGCAGGAT ACGCCCAACA AGAAGAAGCG AACGGAGCAG 
GAGTCCAACG AGCCAAGCGC CCATGAGGAG AATGCCATCA AGGAGAAGCG CAAGCGACCG 
GACAGCCCGG TTGAGAGTAT GAACTCGGAT AGCAGACCGG ATTCAGTGCT CGACGATGGG 
GAATCGAATA CCACGGACAC CACCACCGCC GAGCAGCAGT CCACAAAGGA CAGCAAGGAG 
ACGGTCAGCT GCAAGGAGGA GCGCGAAATG GTCACCAACG ATCTGGAGGC CAAGGCCGAG 
GAAAAGGCCA TCAAGGCAGA GGCTTTGGCC GAAGACAGCA AGGATAGCGC CATCAAGAAC 
ATGGACGAGG AGACAAACAT CCAGGCGCCT AGCAGTGCAG ACACTAGTTT GGTGGATGGT 
CCTAATCCCA ATGCCCTGCC CAGTCCGGTA GCCGCACCAA TCACAATGAA GGTACCAACA 
ATTGCCACCG TTGAGGCGCT GAACGCGTCC GTCGACCGCA AGGAGGCCAT CGAGAAGATG 
GAGTCGTGCG ACAGCGATCC GGAGATGCTT AAAAAACTGG CAACCATTAA GCAGGAAGTA 
TCTCCGCAGC AGCAACAGCA CATGCAACAG CAATCACAGC AGCAGATGCA GCAGCAACTC 
GCTCCAGTTG GCATACCGCA ACCTCCGTCT TGCCCGCCAT CGGAATCAGT CTATATCAAA 
AAAGAGCCCA TGGAGGACTC GATGGACGCC ACCTGCAATC AGAACAGCAA CGAACCGCAG 
GACCTGAAGG TGAAGATCGA GATTAAAAAC GAGGATGCAT TGAAGCATAG TGCTGGAGGT 
CTGCCGCCTT CAGGTCCGTG TGCACCGCCT TCAGCTCTAC ATCCGCTTTC CGGAGCTCCG 
GTAGAGAGCG GCCAGGAGCC ACTGCACCTG CAACACATGC CTCATGGGCA AGTAACGACG 
CAACCGCCCC CTGGCTACCT AATTGATGGC CAGCTAAAGT ATGGACCATC GGGACAAGGC 
GTGCCTCCAC AGCCACCACA ACTGCACAGC GATGCGGCTG GAGGAGTCAG CGGAGCACCA 
CCTGGAGCGC CAACCACGCC CCAGAAGTAT CCGCCCGAGA TGGAGATGAA GTTCGCTCCT 
CAGGATCTCA AGTATCCCCC ACCGCCGCCT CTAGATGCAC TCAAGTACAG CCAGGAGATG 
CAAGCTGCGG CGGCGGCAGC GGCTGCTGCT GGCAAATACG ATATGAAGTA TATGATGGAA 
CAGCAGGGCA AGTACAATGT GGAGTTGTCA GCTGCCCATC AGCCGCCAAG CAAGCCAGGC 
TACCAGGACT CGCTTAAGAT ACCCGATATT AAGCCCGGTT TCGGCCACCT GCCGCACAAC 
GTGGGCTCTC CGCTGGACGC CGCCCATAAA TACGGACCGC CTCCGACGTC GCAAGAGTCC 
CAGCAACAGC AACCCCAGCC GCCGGCACAT CAGGTACCGC CGGGAGCAAC TCCACCACCT 
GGTATCGCCA TGCCCAAGCC GCACTATCAA CACGATGTGC AAACACCACC GTTGGGACGG 
CCCTTCGAGC CGACCGGACT TATGCTCAAG TATGGCGATC CATTGGCAGC CAAATACGGC 
CCGCCCCAGG ATCTCAAGTA CCCGATGCCT CCGGTCTCTC AGGCGGGACC AGCGGACGTA 
AAGCCCTATG GCGGCGAGAA TCTGATCAAG TCCTCACCGT ACGGACCGCC GCCGGAGAGT 
CCTATCGATG CCTCTGCGCG CTCTACACCT GGTCAGGACA GCCAGGGCAG CAATAGCAAT 
TCACAGCCGC CCTCAATGCC CCCGCAACCG CAGCAGTTCC AGTCGCCGCA TCCCTCGCCG 
CATATGCCTT CGCCAGCAGG AGGTGGTCTA CCACCGGGAA TGCATCCGCA AAATCTCATC 
CACGGCCCGC CACCAGGTGC AGCGGGTGGC AGTGGTCCCC AGCCACCTCC ACCGCCCACA 
TCGCTACACC AGCCAACGCC CACGTCTGCA GGTCCACCCA GTCTGCAACA TGGACTACAT 
CCTGGCCATC AACACTCACA GCTGTCTGTG GCATCATCGA TACCGCCGAG CTCGATTGGA 
ATTCCTCCCA CGCTCTCGAC TATGGCGCCC TCGCACATGC ACCCGCACCT TCATCCACAT 
GCGCATCTGC AGGGTCTCCA TCGGCCGCAC GATCTGCCGC CCAGTATGCA TCCACATGCA 
CCCATGCCGC TGTCGTTGCA GGGACATCCG CAGCACGGAC ATGGATTGCC GCCCTCGCAC 
ACTTCTCAGC AACAGCAGCA GCAGCAACAA CAACAACAGC CCGGCGGACC AGCTGGTACG 
GTGCGAACTC CGTCACCAGC CCAGCAGCCG CCGAGATCCA TGCACGATCC GCAATCGTCT 
CGAGAGCCGC CCACATCGCA GCCTTCGACC ACTATGGCAG GATCGAGTGG TCCGGGTGGA 
CCACCGCCGC AACAGTCGCC GCATGCGCAT CGCACATCAC CGTTGCCAGG GCTCGCGGGT 
AGTGGTCCTC CACCCCCGGG ACTCATCGGT CATCCGATGG CCATACACCC GCACCTAGCC 
CACTTGCCGC CCGGACATCC CGCTCACGCA GCGCTGGCCC ATCCTGGACA CCATCTGCTG 
TCGCACTCGA TAGCGGGCTT GGGTCCTGGT GGTGGACCCA TCGCGTTGCT GGCCGGTCCC 
GGCGGTCTTG GTGGTATTCC AGAGTCCGCT CTAAGTCGCC GCACCCCGCC CTCACATCTG 
CCACACTCGC ATGCCTCTTC GGCCCCACTG ACGGCTCACT CGGTGGCCAG TATGACGTCT 
ACCAGTATGT CGCTGACCAC CAGCACGGTG CCATCGTCCG CCTTTAGCCG CGCCAGTCCC 
AGCGTACAGA TCTCGAGCAG TGGAGGAGGA CCTTCAGGGC CCGGAAGCGT TGGACCTGGA 
GGAATGCCAA ACTCGTCGGC AGCAGCGGCT GCTGCGGCAG CTGCTCATCG GGCAGCCTCA 
CCGGCATCCA GCGTAAGCAG CCTAAGTCGG CAGAGTCCGC TGCATCCGGT GCCGCAGTCG 
CCGCTCAGCC ATCATCCCTC GTCCTCTGCG TTATCCGCCG CAGCAGCTGC TGTGGCGGAG 
CGGGATCGAC ATGCGCTGAT GCGTCAGCAA TCGCCACACA TGACTCCACC CCCGGTGTCC 
AACGCCTCTT TAATGGCGAG TCCTCTGAGC AAGATGTACG CTCCTCAACC GGGTCAGAGA 
GGCTTGGGAA CATCACCGCC ACCGCATTTG CGGCCAGGAG CATCGCCGCC GGTCATTCGT 
CACCCGCAGA TGCCTCTACC GTTGCCACTG ATTGCGCCTG GCGGAGGAAT CCCGCAGATT 
GGAGTGCATC CGGGTCAGTC ACCGTATCCG CACCCGCTTC TGCATCCCTC GGTATTCTAC 
TCGCCGCATC ACCATCCATT TAATTCGCCA TATGGCTATG CGCCCTATGG TCCTGGATTC 
CCGGCATACA TGAAGCCGCC ACCACAGCCG GGACAGCTCG ATCCGGCAGC CGTGATGGCG 
GCCCACCATG CCGGATTGCA AGGACCGCCG CCCCAGCAGA TGCGCCAGGA CGAGCAGAAT 
GCAGCGGCCG CCGCTGCACA AGCAGCTGCT GAGAAACAAC ACCAAGCGGC TGCAGCAGCG 
GCAGCCCAGC AGCACAAGGC GCCGCAACAA CAACAGCCTG GCGGAATGCC ACCCAACAAA 
CCGCCGACGC CAAAGACGCC ACAGGGTCCG GGCGGTGGGA TGCCCCCAGG AATGGGTGGA 
CCGGGAACAC CGACGGGACT GCCGCCAGGT GCCTATCCTG GCAGCCATAT GCCGGGATAT 
CCACAAGGGC CGCCTCATGG GTCACCCTTT GCGCCACAAG ATGGTCAGCC TCACGGCTTG 
AAGCCCACAT CGCACATGGA CGCCCTGCGA GCGCATGCAC ACTCAGCCAA CTCGGCGGGT 
ATGGGCGGTG GACACCATCC GACGGAGCCA TTGCCCATTG ATATTGAACC GGATCCAGAG 
CCAGAGATTC CCAGTCCAAC GCACAATATA CCACGTGGTC CCAGTCCCGA AGCAAAACCG 
GACGACACCG AATGCCATCG CTCTCAGTCT GCCATATTTG TGCGCCACAT CGATCGTGGG 
GATTACAATT CGTGCACGAG AACAGATTTG ATCTTCAAGC CGGTGGCCGA CTCAAAGTTG 
GCCCGCAAGC GTGAAGAACG CGACCGCAAG CTGGCCGAAA AGGAGCGTGA GCGGCGACAG 
CAGCAGCAGC AACAACAACA GCAGCAGCAA CAACAGCAAG CAGCGGCCGC GCAACAGGCG 
GCACAGCAAG CCAAGATGAA GGCTGAGCTG AAGCCACCGT ATGCGGATAC GCCGGCACTG 
CGTCAACTGT CCGAGTACGC TCGTCCCCAC GTCGCCTTCA GGGAACTGGA GGAGATTAAA 
AACGCACAAG CTGCTGCGGC GAGTCAATCC CGACTAGATC CGCACTGGAT GGAATACTAT 
CGACGCGGCA TCCACCCCTC GCAGTTCCCG CTGTATGCGA ATCCGGCGAT ATCGCAGATG 
GAGAGGGAGC GTCTGGGAAT TCCACCTCCG CACCATGTGG GGTTGGACCC GGGCGAGCAC 
ATGATACGAT TGACGAGAGA ATATCATGCA CACTCTCATA CTCATTTACA TTTGCCTTTG 
CATCCACAGC CGCAACCACC GGAGGCCGGT TTCCAACTGC CACCGAATGT TGGCCAGTAT 
CCGCGGCCAA ATATGCTTAT ACCTAGGGAG CCGCACTCGG ATGTCCTGCT GCGCATGTCC 
TATGCCGACC AACTACAGTA TTTACAGGCC GCCGAGTTTC AGCGACAGTC CCTGCATGAT 
CAGTACTTTA GACAACGGCC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gug ( NM_001274651.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261580.1 Drosophila melanogaster grunge, transcript variant I (Gug), mRNA.

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CloneID	OFa19920
Clone ID Related Accession (Same CDS sequence)	NM_001274651.1
Accession Version	NM_001274651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grunge, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGC AAAACTGCCC 
GATTATAATC CAATCTCAAG CTTCCCCATC GACAAGGAAA CCGATGAACG TGAACTAGAG 
GAATCAAGAT GGAGTCCAGG CGTTGTGGCC GATGGCGACT TGTTAATGTT CTTGCGTGCG 
GCTCGATCCA TGGCTGCATT TCAAGGAATG TGTGATGGTG GTTTAGAAGA CGGTTGTTTG 
GCTGCTAGTC GCGACGACAC TACAATAAAC GCACTCGACG TGCTCCACGA TTCTGGCTAC 
GATCCAGGCA AAGCTCTACA AGCGCTCGTA AAGTGCCCCG TTTCGAAGGG CATCGACAAG 
AAGTGGACCG AGGACGAAAC AAAGAAATTC ATCAAGGGTC TGCGTCAGTT TGGGAAGAAC 
TTCTTCCGCA TCCATAAGGA CTTGCTGCCG CACAAGGACA CGCCGGAGCT GGTCGAGTTC 
TACTATCTGT GGAAGAAGAC GCCCGGCGCG AACAATAATC GGCCACACAG GCGACGCCGC 
CAAAGCGCCC TGCGACGCAA CCGTGTCACG CGGGCCAACA ACAGCAACAG CAACACTCCT 
CCGAAGAAGG AGGACACTCC AGAACCACAA ACTGCGACGA CGGCGACGGC GGCGGCAACC 
GCGGCGTCCG AGACGGCGAG TCGCTCCTCG CCCGCTGTCT CCAAGGAGGA GAACAGCTCG 
CTCACCGAGG ACGACGCCAG CGAGTGCGAC AGTGATTCGA GTCTGACCCA CAAAAGGGAT 
GAATCACCCT CAAGGATGAG GACGCGTAAC AAGCAACAGA ACAACAACAG CAGCACCAGC 
AGCGGTAACA ACACGGCCGG CAACGGTGGC GGTAACGCCA CATCCATAAG CAGCGGATCA 
ACCGGCGGCG GTGCCGCTGG CGGCAATAGT TCGTCTAAGG ATCAATCAGC CAACGCCGTG 
GCTAATGGCA AGCGACCCAA GAGGGGCTCC GAAACACCGG ACGTTTCCGG CGGAGCCTCG 
GTCGATAGTC CCAAGACACC GACGAAGGCT GTGGCCGAGA GTTCAGCCAA TAAGCGCAAG 
GGTGGCAAGC AGGATACGCC CAACAAGAAG AAGCGAACGG AGCAGGAGTC CAACGAGCCA 
AGCGCCCATG AGGAGAATGC CATCAAGGAG AAGCGCAAGC GACCGGACAG CCCGGTTGAG 
AGTATGAACT CGGATAGCAG ACCGGATTCA GTGCTCGACG ATGGGGAATC GAATACCACG 
GACACCACCA CCGCCGAGCA GCAGTCCACA AAGGACAGCA AGGAGACGGT CAGCTGCAAG 
GAGGAGCGCG AAATGGTCAC CAACGATCTG GAGGCCAAGG CCGAGGAAAA GGCCATCAAG 
GCAGAGGCTT TGGCCGAAGA CAGCAAGGAT AGCGCCATCA AGAACATGGA CGAGGAGACA 
AACATCCAGG CGCCTAGCAG TGCAGACACT AGTTTGGTGG ATGGTCCTAA TCCCAATGCC 
CTGCCCAGTC CGGTAGCCGC ACCAATCACA ATGAAGGTAC CAACAATTGC CACCGTTGAG 
GCGCTGAACG CGTCCGTCGA CCGCAAGGAG GCCATCGAGA AGATGGAGTC GTGCGACAGC 
GATCCGGAGA TGCTTAAAAA ACTGGCAACC ATTAAGCAGG AAGTATCTCC GCAGCAGCAA 
CAGCACATGC AACAGCAATC ACAGCAGCAG ATGCAGCAGC AACTCGCTCC AGTTGGCATA 
CCGCAACCTC CGTCTTGCCC GCCATCGGAA TCAGTCTATA TCAAAAAAGA GCCCATGGAG 
GACTCGATGG ACGCCACCTG CAATCAGAAC AGCAACGAAC CGCAGGACCT GAAGGTGAAG 
ATCGAGATTA AAAACGAGGA TGCATTGAAG CATAGTGCTG GAGGTCTGCC GCCTTCAGGT 
CCGTGTGCAC CGCCTTCAGC TCTACATCCG CTTTCCGGAG CTCCGGTAGA GAGCGGCCAG 
GAGCCACTGC ACCTGCAACA CATGCCTCAT GGGCAAGTAA CGACGCAACC GCCCCCTGGC 
TACCTAATTG ATGGCCAGCT AAAGTATGGA CCATCGGGAC AAGGCGTGCC TCCACAGCCA 
CCACAACTGC ACAGCGATGC GGCTGGAGGA GTCAGCGGAG CACCACCTGG AGCGCCAACC 
ACGCCCCAGA AGTATCCGCC CGAGATGGAG ATGAAGTTCG CTCCTCAGGA TCTCAAGTAT 
CCCCCACCGC CGCCTCTAGA TGCACTCAAG TACAGCCAGG AGATGCAAGC TGCGGCGGCG 
GCAGCGGCTG CTGCTGGCAA ATACGATATG AAGTATATGA TGGAACAGCA GGGCAAGTAC 
AATGTGGAGT TGTCAGCTGC CCATCAGCCG CCAAGCAAGC CAGGCTACCA GGACTCGCTT 
AAGATACCCG ATATTAAGCC CGGTTTCGGC CACCTGCCGC ACAACGTGGG CTCTCCGCTG 
GACGCCGCCC ATAAATACGG ACCGCCTCCG ACGTCGCAAG AGTCCCAGCA ACAGCAACCC 
CAGCCGCCGG CACATCAGGT ACCGCCGGGA GCAACTCCAC CACCTGGTAT CGCCATGCCC 
AAGCCGCACT ATCAACACGA TGTGCAAACA CCACCGTTGG GACGGCCCTT CGAGCCGACC 
GGACTTATGC TCAAGTATGG CGATCCATTG GCAGCCAAAT ACGGCCCGCC CCAGGATCTC 
AAGTACCCGA TGCCTCCGGT CTCTCAGGCG GGACCAGCGG ACGTAAAGCC CTATGGCGGC 
GAGAATCTGA TCAAGTCCTC ACCGTACGGA CCGCCGCCGG AGAGTCCTAT CGATGCCTCT 
GCGCGCTCTA CACCTGGTCA GGACAGCCAG GGCAGCAATA GCAATTCACA GCCGCCCTCA 
ATGCCCCCGC AACCGCAGCA GTTCCAGTCG CCGCATCCCT CGCCGCATAT GCCTTCGCCA 
GCAGGAGGTG GTCTACCACC GGGAATGCAT CCGCAAAATC TCATCCACGG CCCGCCACCA 
GGTGCAGCGG GTGGCAGTGG TCCCCAGCCA CCTCCACCGC CCACATCGCT ACACCAGCCA 
ACGCCCACGT CTGCAGGTCC ACCCAGTCTG CAACATGGAC TACATCCTGG CCATCAACAC 
TCACAGCTGT CTGTGGCATC ATCGATACCG CCGAGCTCGA TTGGAATTCC TCCCACGCTC 
TCGACTATGG CGCCCTCGCA CATGCACCCG CACCTTCATC CACATGCGCA TCTGCAGGGT 
CTCCATCGGC CGCACGATCT GCCGCCCAGT ATGCATCCAC ATGCACCCAT GCCGCTGTCG 
TTGCAGGGAC ATCCGCAGCA CGGACATGGA TTGCCGCCCT CGCACACTTC TCAGCAACAG 
CAGCAGCAGC AACAACAACA ACAGCCCGGC GGACCAGCTG GTACGGTGCG AACTCCGTCA 
CCAGCCCAGC AGCCGCCGAG ATCCATGCAC GATCCGCAAT CGTCTCGAGA GCCGCCCACA 
TCGCAGCCTT CGACCACTAT GGCAGGATCG AGTGGTCCGG GTGGACCACC GCCGCAACAG 
TCGCCGCATG CGCATCGCAC ATCACCGTTG CCAGGGCTCG CGGGTAGTGG TCCTCCACCC 
CCGGGACTCA TCGGTCATCC GATGGCCATA CACCCGCACC TAGCCCACTT GCCGCCCGGA 
CATCCCGCTC ACGCAGCGCT GGCCCATCCT GGACACCATC TGCTGTCGCA CTCGATAGCG 
GGCTTGGGTC CTGGTGGTGG ACCCATCGCG TTGCTGGCCG GTCCCGGCGG TCTTGGTGGT 
ATTCCAGAGT CCGCTCTAAG TCGCCGCACC CCGCCCTCAC ATCTGCCACA CTCGCATGCC 
TCTTCGGCCC CACTGACGGC TCACTCGGTG GCCAGTATGA CGTCTACCAG TATGTCGCTG 
ACCACCAGCA CGGTGCCATC GTCCGCCTTT AGCCGCGCCA GTCCCAGCGT ACAGATCTCG 
AGCAGTGGAG GAGGACCTTC AGGGCCCGGA AGCGTTGGAC CTGGAGGAAT GCCAAACTCG 
TCGGCAGCAG CGGCTGCTGC GGCAGCTGCT CATCGGGCAG CCTCACCGGC ATCCAGCGTA 
AGCAGCCTAA GTCGGCAGAG TCCGCTGCAT CCGGTGCCGC AGTCGCCGCT CAGCCATCAT 
CCCTCGTCCT CTGCGTTATC CGCCGCAGCA GCTGCTGTGG CGGAGCGGGA TCGACATGCG 
CTGATGCGTC AGCAATCGCC ACACATGACT CCACCCCCGG TGTCCAACGC CTCTTTAATG 
GCGAGTCCTC TGAGCAAGAT GTACGCTCCT CAACCGGGTC AGAGAGGCTT GGGAACATCA 
CCGCCACCGC ATTTGCGGCC AGGAGCATCG CCGCCGGTCA TTCGTCACCC GCAGATGCCT 
CTACCGTTGC CACTGATTGC GCCTGGCGGA GGAATCCCGC AGATTGGAGT GCATCCGGGT 
CAGTCACCGT ATCCGCACCC GCTTCTGCAT CCCTCGGTAT TCTACTCGCC GCATCACCAT 
CCATTTAATT CGCCATATGG CTATGCGCCC TATGGTCCTG GATTCCCGGC ATACATGAAG 
CCGCCACCAC AGCCGGGACA GCTCGATCCG GCAGCCGTGA TGGCGGCCCA CCATGCCGGA 
TTGCAAGGAC CGCCGCCCCA GCAGATGCGC CAGGACGAGC AGAATGCAGC GGCCGCCGCT 
GCACAAGCAG CTGCTGAGAA ACAACACCAA GCGGCTGCAG CAGCGGCAGC CCAGCAGCAC 
AAGGCGCCGC AACAACAACA GCCTGGCGGA ATGCCACCCA ACAAACCGCC GACGCCAAAG 
ACGCCACAGG GTCCGGGCGG TGGGATGCCC CCAGGAATGG GTGGACCGGG AACACCGACG 
GGACTGCCGC CAGGTGCCTA TCCTGGCAGC CATATGCCGG GATATCCACA AGGGCCGCCT 
CATGGGTCAC CCTTTGCGCC ACAAGATGGT CAGCCTCACG GCTTGAAGCC CACATCGCAC 
ATGGACGCCC TGCGAGCGCA TGCACACTCA GCCAACTCGG CGGGTATGGG CGGTGGACAC 
CATCCGACGG AGCCATTGCC CATTGATATT GAACCGGATC CAGAGCCAGA GATTCCCAGT 
CCAACGCACA ATATACCACG TGGTCCCAGT CCCGAAGCAA AACCGGACGA CACCGAATGC 
CATCGCTCTC AGTCTGCCAT ATTTGTGCGC CACATCGATC GTGGGGATTA CAATTCGTGC 
ACGAGAACAG ATTTGATCTT CAAGCCGGTG GCCGACTCAA AGTTGGCCCG CAAGCGTGAA 
GAACGCGACC GCAAGCTGGC CGAAAAGGAG CGTGAGCGGC GACAGCAGCA GCAGCAACAA 
CAACAGCAGC AGCAACAACA GCAAGCAGCG GCCGCGCAAC AGGCGGCACA GCAAGCCAAG 
ATGAAGGCTG AGCTGAAGCC ACCGTATGCG GATACGCCGG CACTGCGTCA ACTGTCCGAG 
TACGCTCGTC CCCACGTCGC CTTCAGGGAA CTGGAGGAGA TTAAAAACGC ACAAGCTGCT 
GCGGCGAGTC AATCCCGACT AGATCCGCAC TGGATGGAAT ACTATCGACG CGGCATCCAC 
CCCTCGCAGT TCCCGCTGTA TGCGAATCCG GCGATATCGC AGATGGAGAG GGAGCGTCTG 
GGAATTCCAC CTCCGCACCA TGTGGGGTTG GACCCGGGCG AGCACATGAT ACGATTGACG 
AGAGAATATC ATGCACACTC TCATACTCAT TTACATTTGC CTTTGCATCC ACAGCCGCAA 
CCACCGGAGG CCGGTTTCCA ACTGCCACCG AATGTTGGCC AGTATCCGCG GCCAAATATG 
CTTATACCTA GGGAGCCGCA CTCGGATGTC CTGCTGCGCA TGTCCTATGC CGACCAACTA 
CAGTATTTAC AGGCCGCCGA GTTTCAGCGA CAGTCCCTGC ATGATCAGTA CTTTAGACAA 
CGGCCCAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261580.1
CDS	960..6911
Translation	MAASTQGEIRVGPGHQAKLPDYNPISSFPIDKETDERELEESRWSPGVVADGDLLMFLRAARSMAAFQGMCDGGLEDGCLAASRDDTTINALDVLHDSGYDPGKALQALVKCPVSKGIDKKWTEDETKKFIKGLRQFGKNFFRIHKDLLPHKDTPELVEFYYLWKKTPGANNNRPHRRRRQSALRRNRVTRANNSNSNTPPKKEDTPEPQTATTATAAATAASETASRSSPAVSKEENSSLTEDDASECDSDSSLTHKRDESPSRMRTRNKQQNNNSSTSSGNNTAGNGGGNATSISSGSTGGGAAGGNSSSKDQSANAVANGKRPKRGSETPDVSGGASVDSPKTPTKAVAESSANKRKGGKQDTPNKKKRTEQESNEPSAHEENAIKEKRKRPDSPVESMNSDSRPDSVLDDGESNTTDTTTAEQQSTKDSKETVSCKEEREMVTNDLEAKAEEKAIKAEALAEDSKDSAIKNMDEETNIQAPSSADTSLVDGPNPNALPSPVAAPITMKVPTIATVEALNASVDRKEAIEKMESCDSDPEMLKKLATIKQEVSPQQQQHMQQQSQQQMQQQLAPVGIPQPPSCPPSESVYIKKEPMEDSMDATCNQNSNEPQDLKVKIEIKNEDALKHSAGGLPPSGPCAPPSALHPLSGAPVESGQEPLHLQHMPHGQVTTQPPPGYLIDGQLKYGPSGQGVPPQPPQLHSDAAGGVSGAPPGAPTTPQKYPPEMEMKFAPQDLKYPPPPPLDALKYSQEMQAAAAAAAAAGKYDMKYMMEQQGKYNVELSAAHQPPSKPGYQDSLKIPDIKPGFGHLPHNVGSPLDAAHKYGPPPTSQESQQQQPQPPAHQVPPGATPPPGIAMPKPHYQHDVQTPPLGRPFEPTGLMLKYGDPLAAKYGPPQDLKYPMPPVSQAGPADVKPYGGENLIKSSPYGPPPESPIDASARSTPGQDSQGSNSNSQPPSMPPQPQQFQSPHPSPHMPSPAGGGLPPGMHPQNLIHGPPPGAAGGSGPQPPPPPTSLHQPTPTSAGPPSLQHGLHPGHQHSQLSVASSIPPSSIGIPPTLSTMAPSHMHPHLHPHAHLQGLHRPHDLPPSMHPHAPMPLSLQGHPQHGHGLPPSHTSQQQQQQQQQQQPGGPAGTVRTPSPAQQPPRSMHDPQSSREPPTSQPSTTMAGSSGPGGPPPQQSPHAHRTSPLPGLAGSGPPPPGLIGHPMAIHPHLAHLPPGHPAHAALAHPGHHLLSHSIAGLGPGGGPIALLAGPGGLGGIPESALSRRTPPSHLPHSHASSAPLTAHSVASMTSTSMSLTTSTVPSSAFSRASPSVQISSSGGGPSGPGSVGPGGMPNSSAAAAAAAAAHRAASPASSVSSLSRQSPLHPVPQSPLSHHPSSSALSAAAAAVAERDRHALMRQQSPHMTPPPVSNASLMASPLSKMYAPQPGQRGLGTSPPPHLRPGASPPVIRHPQMPLPLPLIAPGGGIPQIGVHPGQSPYPHPLLHPSVFYSPHHHPFNSPYGYAPYGPGFPAYMKPPPQPGQLDPAAVMAAHHAGLQGPPPQQMRQDEQNAAAAAAQAAAEKQHQAAAAAAAQQHKAPQQQQPGGMPPNKPPTPKTPQGPGGGMPPGMGGPGTPTGLPPGAYPGSHMPGYPQGPPHGSPFAPQDGQPHGLKPTSHMDALRAHAHSANSAGMGGGHHPTEPLPIDIEPDPEPEIPSPTHNIPRGPSPEAKPDDTECHRSQSAIFVRHIDRGDYNSCTRTDLIFKPVADSKLARKREERDRKLAEKERERRQQQQQQQQQQQQQQAAAAQQAAQQAKMKAELKPPYADTPALRQLSEYARPHVAFRELEEIKNAQAAAASQSRLDPHWMEYYRRGIHPSQFPLYANPAISQMERERLGIPPPHHVGLDPGEHMIRLTREYHAHSHTHLHLPLHPQPQPPEAGFQLPPNVGQYPRPNMLIPREPHSDVLLRMSYADQLQYLQAAEFQRQSLHDQYFRQRPR

Target ORF information:

RefSeq Version	NM_001274651.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grunge, transcript variant I (Gug), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274651.1

ORF Insert Sequence:

ATGGCGGCCT CCACTCAAGG AGAAATTCGA GTGGGTCCCG GCCACCAGGC AAAACTGCCC 
GATTATAATC CAATCTCAAG CTTCCCCATC GACAAGGAAA CCGATGAACG TGAACTAGAG 
GAATCAAGAT GGAGTCCAGG CGTTGTGGCC GATGGCGACT TGTTAATGTT CTTGCGTGCG 
GCTCGATCCA TGGCTGCATT TCAAGGAATG TGTGATGGTG GTTTAGAAGA CGGTTGTTTG 
GCTGCTAGTC GCGACGACAC TACAATAAAC GCACTCGACG TGCTCCACGA TTCTGGCTAC 
GATCCAGGCA AAGCTCTACA AGCGCTCGTA AAGTGCCCCG TTTCGAAGGG CATCGACAAG 
AAGTGGACCG AGGACGAAAC AAAGAAATTC ATCAAGGGTC TGCGTCAGTT TGGGAAGAAC 
TTCTTCCGCA TCCATAAGGA CTTGCTGCCG CACAAGGACA CGCCGGAGCT GGTCGAGTTC 
TACTATCTGT GGAAGAAGAC GCCCGGCGCG AACAATAATC GGCCACACAG GCGACGCCGC 
CAAAGCGCCC TGCGACGCAA CCGTGTCACG CGGGCCAACA ACAGCAACAG CAACACTCCT 
CCGAAGAAGG AGGACACTCC AGAACCACAA ACTGCGACGA CGGCGACGGC GGCGGCAACC 
GCGGCGTCCG AGACGGCGAG TCGCTCCTCG CCCGCTGTCT CCAAGGAGGA GAACAGCTCG 
CTCACCGAGG ACGACGCCAG CGAGTGCGAC AGTGATTCGA GTCTGACCCA CAAAAGGGAT 
GAATCACCCT CAAGGATGAG GACGCGTAAC AAGCAACAGA ACAACAACAG CAGCACCAGC 
AGCGGTAACA ACACGGCCGG CAACGGTGGC GGTAACGCCA CATCCATAAG CAGCGGATCA 
ACCGGCGGCG GTGCCGCTGG CGGCAATAGT TCGTCTAAGG ATCAATCAGC CAACGCCGTG 
GCTAATGGCA AGCGACCCAA GAGGGGCTCC GAAACACCGG ACGTTTCCGG CGGAGCCTCG 
GTCGATAGTC CCAAGACACC GACGAAGGCT GTGGCCGAGA GTTCAGCCAA TAAGCGCAAG 
GGTGGCAAGC AGGATACGCC CAACAAGAAG AAGCGAACGG AGCAGGAGTC CAACGAGCCA 
AGCGCCCATG AGGAGAATGC CATCAAGGAG AAGCGCAAGC GACCGGACAG CCCGGTTGAG 
AGTATGAACT CGGATAGCAG ACCGGATTCA GTGCTCGACG ATGGGGAATC GAATACCACG 
GACACCACCA CCGCCGAGCA GCAGTCCACA AAGGACAGCA AGGAGACGGT CAGCTGCAAG 
GAGGAGCGCG AAATGGTCAC CAACGATCTG GAGGCCAAGG CCGAGGAAAA GGCCATCAAG 
GCAGAGGCTT TGGCCGAAGA CAGCAAGGAT AGCGCCATCA AGAACATGGA CGAGGAGACA 
AACATCCAGG CGCCTAGCAG TGCAGACACT AGTTTGGTGG ATGGTCCTAA TCCCAATGCC 
CTGCCCAGTC CGGTAGCCGC ACCAATCACA ATGAAGGTAC CAACAATTGC CACCGTTGAG 
GCGCTGAACG CGTCCGTCGA CCGCAAGGAG GCCATCGAGA AGATGGAGTC GTGCGACAGC 
GATCCGGAGA TGCTTAAAAA ACTGGCAACC ATTAAGCAGG AAGTATCTCC GCAGCAGCAA 
CAGCACATGC AACAGCAATC ACAGCAGCAG ATGCAGCAGC AACTCGCTCC AGTTGGCATA 
CCGCAACCTC CGTCTTGCCC GCCATCGGAA TCAGTCTATA TCAAAAAAGA GCCCATGGAG 
GACTCGATGG ACGCCACCTG CAATCAGAAC AGCAACGAAC CGCAGGACCT GAAGGTGAAG 
ATCGAGATTA AAAACGAGGA TGCATTGAAG CATAGTGCTG GAGGTCTGCC GCCTTCAGGT 
CCGTGTGCAC CGCCTTCAGC TCTACATCCG CTTTCCGGAG CTCCGGTAGA GAGCGGCCAG 
GAGCCACTGC ACCTGCAACA CATGCCTCAT GGGCAAGTAA CGACGCAACC GCCCCCTGGC 
TACCTAATTG ATGGCCAGCT AAAGTATGGA CCATCGGGAC AAGGCGTGCC TCCACAGCCA 
CCACAACTGC ACAGCGATGC GGCTGGAGGA GTCAGCGGAG CACCACCTGG AGCGCCAACC 
ACGCCCCAGA AGTATCCGCC CGAGATGGAG ATGAAGTTCG CTCCTCAGGA TCTCAAGTAT 
CCCCCACCGC CGCCTCTAGA TGCACTCAAG TACAGCCAGG AGATGCAAGC TGCGGCGGCG 
GCAGCGGCTG CTGCTGGCAA ATACGATATG AAGTATATGA TGGAACAGCA GGGCAAGTAC 
AATGTGGAGT TGTCAGCTGC CCATCAGCCG CCAAGCAAGC CAGGCTACCA GGACTCGCTT 
AAGATACCCG ATATTAAGCC CGGTTTCGGC CACCTGCCGC ACAACGTGGG CTCTCCGCTG 
GACGCCGCCC ATAAATACGG ACCGCCTCCG ACGTCGCAAG AGTCCCAGCA ACAGCAACCC 
CAGCCGCCGG CACATCAGGT ACCGCCGGGA GCAACTCCAC CACCTGGTAT CGCCATGCCC 
AAGCCGCACT ATCAACACGA TGTGCAAACA CCACCGTTGG GACGGCCCTT CGAGCCGACC 
GGACTTATGC TCAAGTATGG CGATCCATTG GCAGCCAAAT ACGGCCCGCC CCAGGATCTC 
AAGTACCCGA TGCCTCCGGT CTCTCAGGCG GGACCAGCGG ACGTAAAGCC CTATGGCGGC 
GAGAATCTGA TCAAGTCCTC ACCGTACGGA CCGCCGCCGG AGAGTCCTAT CGATGCCTCT 
GCGCGCTCTA CACCTGGTCA GGACAGCCAG GGCAGCAATA GCAATTCACA GCCGCCCTCA 
ATGCCCCCGC AACCGCAGCA GTTCCAGTCG CCGCATCCCT CGCCGCATAT GCCTTCGCCA 
GCAGGAGGTG GTCTACCACC GGGAATGCAT CCGCAAAATC TCATCCACGG CCCGCCACCA 
GGTGCAGCGG GTGGCAGTGG TCCCCAGCCA CCTCCACCGC CCACATCGCT ACACCAGCCA 
ACGCCCACGT CTGCAGGTCC ACCCAGTCTG CAACATGGAC TACATCCTGG CCATCAACAC 
TCACAGCTGT CTGTGGCATC ATCGATACCG CCGAGCTCGA TTGGAATTCC TCCCACGCTC 
TCGACTATGG CGCCCTCGCA CATGCACCCG CACCTTCATC CACATGCGCA TCTGCAGGGT 
CTCCATCGGC CGCACGATCT GCCGCCCAGT ATGCATCCAC ATGCACCCAT GCCGCTGTCG 
TTGCAGGGAC ATCCGCAGCA CGGACATGGA TTGCCGCCCT CGCACACTTC TCAGCAACAG 
CAGCAGCAGC AACAACAACA ACAGCCCGGC GGACCAGCTG GTACGGTGCG AACTCCGTCA 
CCAGCCCAGC AGCCGCCGAG ATCCATGCAC GATCCGCAAT CGTCTCGAGA GCCGCCCACA 
TCGCAGCCTT CGACCACTAT GGCAGGATCG AGTGGTCCGG GTGGACCACC GCCGCAACAG 
TCGCCGCATG CGCATCGCAC ATCACCGTTG CCAGGGCTCG CGGGTAGTGG TCCTCCACCC 
CCGGGACTCA TCGGTCATCC GATGGCCATA CACCCGCACC TAGCCCACTT GCCGCCCGGA 
CATCCCGCTC ACGCAGCGCT GGCCCATCCT GGACACCATC TGCTGTCGCA CTCGATAGCG 
GGCTTGGGTC CTGGTGGTGG ACCCATCGCG TTGCTGGCCG GTCCCGGCGG TCTTGGTGGT 
ATTCCAGAGT CCGCTCTAAG TCGCCGCACC CCGCCCTCAC ATCTGCCACA CTCGCATGCC 
TCTTCGGCCC CACTGACGGC TCACTCGGTG GCCAGTATGA CGTCTACCAG TATGTCGCTG 
ACCACCAGCA CGGTGCCATC GTCCGCCTTT AGCCGCGCCA GTCCCAGCGT ACAGATCTCG 
AGCAGTGGAG GAGGACCTTC AGGGCCCGGA AGCGTTGGAC CTGGAGGAAT GCCAAACTCG 
TCGGCAGCAG CGGCTGCTGC GGCAGCTGCT CATCGGGCAG CCTCACCGGC ATCCAGCGTA 
AGCAGCCTAA GTCGGCAGAG TCCGCTGCAT CCGGTGCCGC AGTCGCCGCT CAGCCATCAT 
CCCTCGTCCT CTGCGTTATC CGCCGCAGCA GCTGCTGTGG CGGAGCGGGA TCGACATGCG 
CTGATGCGTC AGCAATCGCC ACACATGACT CCACCCCCGG TGTCCAACGC CTCTTTAATG 
GCGAGTCCTC TGAGCAAGAT GTACGCTCCT CAACCGGGTC AGAGAGGCTT GGGAACATCA 
CCGCCACCGC ATTTGCGGCC AGGAGCATCG CCGCCGGTCA TTCGTCACCC GCAGATGCCT 
CTACCGTTGC CACTGATTGC GCCTGGCGGA GGAATCCCGC AGATTGGAGT GCATCCGGGT 
CAGTCACCGT ATCCGCACCC GCTTCTGCAT CCCTCGGTAT TCTACTCGCC GCATCACCAT 
CCATTTAATT CGCCATATGG CTATGCGCCC TATGGTCCTG GATTCCCGGC ATACATGAAG 
CCGCCACCAC AGCCGGGACA GCTCGATCCG GCAGCCGTGA TGGCGGCCCA CCATGCCGGA 
TTGCAAGGAC CGCCGCCCCA GCAGATGCGC CAGGACGAGC AGAATGCAGC GGCCGCCGCT 
GCACAAGCAG CTGCTGAGAA ACAACACCAA GCGGCTGCAG CAGCGGCAGC CCAGCAGCAC 
AAGGCGCCGC AACAACAACA GCCTGGCGGA ATGCCACCCA ACAAACCGCC GACGCCAAAG 
ACGCCACAGG GTCCGGGCGG TGGGATGCCC CCAGGAATGG GTGGACCGGG AACACCGACG 
GGACTGCCGC CAGGTGCCTA TCCTGGCAGC CATATGCCGG GATATCCACA AGGGCCGCCT 
CATGGGTCAC CCTTTGCGCC ACAAGATGGT CAGCCTCACG GCTTGAAGCC CACATCGCAC 
ATGGACGCCC TGCGAGCGCA TGCACACTCA GCCAACTCGG CGGGTATGGG CGGTGGACAC 
CATCCGACGG AGCCATTGCC CATTGATATT GAACCGGATC CAGAGCCAGA GATTCCCAGT 
CCAACGCACA ATATACCACG TGGTCCCAGT CCCGAAGCAA AACCGGACGA CACCGAATGC 
CATCGCTCTC AGTCTGCCAT ATTTGTGCGC CACATCGATC GTGGGGATTA CAATTCGTGC 
ACGAGAACAG ATTTGATCTT CAAGCCGGTG GCCGACTCAA AGTTGGCCCG CAAGCGTGAA 
GAACGCGACC GCAAGCTGGC CGAAAAGGAG CGTGAGCGGC GACAGCAGCA GCAGCAACAA 
CAACAGCAGC AGCAACAACA GCAAGCAGCG GCCGCGCAAC AGGCGGCACA GCAAGCCAAG 
ATGAAGGCTG AGCTGAAGCC ACCGTATGCG GATACGCCGG CACTGCGTCA ACTGTCCGAG 
TACGCTCGTC CCCACGTCGC CTTCAGGGAA CTGGAGGAGA TTAAAAACGC ACAAGCTGCT 
GCGGCGAGTC AATCCCGACT AGATCCGCAC TGGATGGAAT ACTATCGACG CGGCATCCAC 
CCCTCGCAGT TCCCGCTGTA TGCGAATCCG GCGATATCGC AGATGGAGAG GGAGCGTCTG 
GGAATTCCAC CTCCGCACCA TGTGGGGTTG GACCCGGGCG AGCACATGAT ACGATTGACG 
AGAGAATATC ATGCACACTC TCATACTCAT TTACATTTGC CTTTGCATCC ACAGCCGCAA 
CCACCGGAGG CCGGTTTCCA ACTGCCACCG AATGTTGGCC AGTATCCGCG GCCAAATATG 
CTTATACCTA GGGAGCCGCA CTCGGATGTC CTGCTGCGCA TGTCCTATGC CGACCAACTA 
CAGTATTTAC AGGCCGCCGA GTTTCAGCGA CAGTCCCTGC ATGATCAGTA CTTTAGACAA 
CGGCCCAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Gug
Entrez Gene ID	46156
Full Name	Grunge
Synonyms	Atro,CG6964,Dmel\CG6964,GUG,atro,gug,l(3)01323,l(3)03928,l(3)S146907,l(3)j5A3,l(3)rO116
General protein information	Preferred Names Grunge Names CG6964 gene product from transcript CG6964-RH CG6964-PA CG6964-PC CG6964-PD CG6964-PE CG6964-PF CG6964-PG CG6964-PH CG6964-PI CG6964-PJ Gug-PA Gug-PC Gug-PD Gug-PE Gug-PF Gug-PG Gug-PH Gug-PI Gug-PJ atrophin grunge
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66C13-66D1\|3-26 cM

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gug cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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