LOC542278 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC542278
Entrez Gene ID	542278
Full Name	nitrate reductase [NADH]-like
Synonyms	GRMZM2G568636,NAR1S,NR,NR1
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305856.1	NP_001292785.1	nitrate reductase [NADH]

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Loci controlling nitrate reductase activity in maize: ultraviolet-B signaling in aerial tissues increases nitrate reductase activity in leaf and root when responsive alleles are present.
Morrison KM, Simmons SJ, Stapleton AE
Physiologia plantarum140(4)334-41(2010 Dec)

Structural studies on corn nitrate reductase: refined structure of the cytochrome b reductase fragment at 2.5 A, its ADP complex and an active-site mutant and modeling of the cytochrome b domain.
Lu G, Lindqvist Y, Schneider G, Dwivedi U, Campbell W
Journal of molecular biology248(5)931-48(1995 May)

Crystal structure of the FAD-containing fragment of corn nitrate reductase at 2.5 A resolution: relationship to other flavoprotein reductases.
Lu G, Campbell WH, Schneider G, Lindqvist Y
Structure (London, England : 1993)2(9)809-21(1994 Sep)

Identification of an "essential" cysteine of nitrate reductase via mutagenesis of its recombinant cytochrome b reductase domain.
Dwivedi UN, Shiraishi N, Campbell WH
The Journal of biological chemistry269(19)13785-91(1994 May)

cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD(P):Glyceraldehyde-3-Phosphate Dehydrogenase : Characterization of the Clones and Analysis of the Expression of the Genes in Leaves as Influenced by Nitrate in the Light and Dark.
Gowri G, Campbell WH
Plant physiology90(3)792-8(1989 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The loci controlling regulation of nitrate reductase activity under ultraviolet-B map to different positions than the loci controlling nitrate reductase activity in unexposed plants.
Title: Loci controlling nitrate reductase activity in maize: ultraviolet-B signaling in aerial tissues increases nitrate reductase activity in leaf and root when responsive alleles are present.

The following LOC542278 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC542278 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OZa22484	NM_001305856.1 Latest version!	Zea mays nitrate reductase [NADH]-like (LOC542278), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC542278 ( NM_001305856.1 ) cDNA ORF clone, Zea mays -> NP_001292785.1 Zea mays nitrate reductase [NADH]-like (LOC542278), mRNA.

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CloneID	OZa22484
Clone ID Related Accession (Same CDS sequence)	NM_001305856.1
Accession Version	NM_001305856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Zea mays
Product	nitrate reductase [NADH]
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LPUQ01001198.1. On Apr 4, 2015 this sequence version replaced XM_008680224.1. ##Evidence-Data-START## Transcript exon combination :: SRR3147048.410.1, SRR3147048.22.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN00780085 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGCTG TGGAGCCCCG GCAGTTCGGG CGTCTGGAGC CGGGCTCCTC GCCAGTGCGC 
GTGGCCACGA ACGGCGCCAA GGCGTACCCT CCTCCGGCAA GCCACCTCCC GCGGCGCGCT 
GACTCCCCTG TCCGCGGCTG CAGCTTCCCT CCCCTCGTCT CCCCGCCGCG CAGGCTGGAC 
GACGCGTCGG ACGACGAGGA CGAGGAGCAG GAGGACTGGC GAGAGCTGTA CGGCTCGCAC 
CTGCAGCTGG AGGTGGAGCC CGCGGTGCAG GACGCGCGCG ACGAAGGCAC CGCGGACGCG 
TGGATCGAGC GGAACCCGTG CCTGGTGAGG CTGACGGGGA AGCACCCGCT CAACTGCGAG 
CCGCCGCTGG CGCGGCTGAT GCACCACGGC TTCATCACCC CGGCGCCGCT GCACTACGTG 
CGCAACCACG GCGCCGTGCC GCGGGGGGAC TGGGCGACGT GGACCGTGGA GGTGACGGGC 
CTCGTGAGGC GGCCCGCGCG GCTCACCATG GAGGAGCTGG CGCGCGACTT CCCCGCCGTG 
GAGATCCCCG TGACGCTGGC GTGCGCGGGC AACCGGCGCA AGGAGCAGAA CATGGTGCAG 
CAGACGGTGG GCTTCAACTG GGGCGCCGCC GGCGTGTCCA CGTCCGTGTG GCGCGGCGCG 
CGCCTCCGCG ACGTGCTCCG CCGCTGCGGC ATCGTGCCCC GCAAGGGCGG CGCCCTCAAC 
GTGTGCTTCG AGGGCGCCGA GGACCTCCCC GGCGGCGGGG GATCCAAGTA CGGCACCAGC 
GTCACGCGCG AGTGGGCCCT GGACCCGTCG CGGGACATCA TGCTCGCCTA CATGCAGAAC 
GGCGAGCCGC TGCTGCCGGA CCACGGCTTC CCCGTGCGCG TCATCATCCC CGGCTGCATC 
GGTGGCCGCA TGGTCAAGTG GCTCAAGCGC ATCATCGTCA CCCCCGCCGA GTCCGACAAT 
TACTACCATT TCAAGGACAA CCGCGTCCTG CCGTCGCACG TCGACGCCGA GCTCGCCAAC 
GCAGAAGCGT GGTGGTACAA GCCGGAGTAC ATCATCAACG AGCTGAACAT AAACTCGGTG 
ATAACGACGC CGGGGCACGA CGAGATCCTG CCCATCAACA GCATTACCAC ACAGCGCGGC 
TACACCATGA AAGGATACGC CTACTCCGGC GGCGGCAAGA AGGTGACGCG GGTGGAGGTG 
ACGCTGGACG GCGGCGAGAC ATGGCTGGTG TGCCACCTCG ACCACCCGGA GAAGCCCAAC 
AAGTACGGCA AGTACTGGTG CTGGTGCTTC TGGTCCGTCG AGGTGGAGGT CCTCGACCTG 
CTCGGCGCCA AGGAGATCGC CGTGCGCGCA TGGGACCAGT CGCTCAACAC CCAGCCCGAG 
AAGCTCATAT GGAACCTCAT GGGGATGATG AACAACTGCT GGTTCAAGGT GAAGGTGAAC 
GTGTGCCGTC CGCACAAGGG CGAGATCGGG CTGGTGTTCG AGCACCCGAC GCAGCCCGGC 
AACCAGCCCG GCGGGTGGAT GGCGCGGCAG AAGCACCTGG AGACGGCGGA GGCGGCCGCG 
CCGGGCCTCA AGCGCAGCAC GTCCACGCCG TTCATGAACA CCACCGACGT CGGCAAGCAG 
TTCACCATGT CCGAGGTGCG CAAGCACGCG TCGCAGGAGT CGGCGTGGAT CGTCGTGCAC 
GGCCACGTCT ACGACTGCAC CAAGTTCCTC AAGGACCACC CGGGCGGCGC CGACAGCATC 
CTCATCAACG CCGGTACCGA CTGCACCGAG GAGTTCGACG CCATCCACTC CGACAAGGCC 
AAGGCGCTCC TCGACACCTA CCGCATCGGC GAGCTCATCA CCACGGGCAC CGGCTACAGC 
TCCGACAACT CCGTCCACGG CGGCTCCGTC CTGTCGCACC TCGCGCCCAT CCGCGAGGCC 
GTCAGGGCTC CCGCGCTCTC CAACCCGCGC GAAAAGATCC ACTGCCGCCT CGTCGGCAAG 
AAGGAGCTGT CCCGCGACGT CCGCCTCTTC CGCTTCTCGC TGCCGTCGCC CGACCAGGTG 
CTCGGCCTCC CCATCGGCAA GCACATCTTC GTCTGCGCCA GTATTGAAGG GAAGCTGTGT 
ATGCGGGCGT ACACGCCCAC GAGCATGGTG GACGAGATCG GCCACTTCGA CCTCCTCGTC 
AAGGTCTACT TCAAGAACGA GCACCCCAAG TTCCCCAACG GCGGCCTCAT GACCCAGTAC 
CTCGACTCGC TTCCCGTCGG CTCCTACATC GACGTCAAGG GCCCGCTTGG CCACGTGGAG 
TACACCGGCC GCGGCAGCTT CGTCATCAAC GGCAAGCAGC GCCATGCGAG CCGCCTCGCC 
ATGATCTGCG GCGGAAGTGG GATCACGCCC ATGTACCAGA TCATCCAGGC GGTGCTGCGC 
GAGCAGCCGG AGGACCACAC GGAGATGCAC CTCGTGTACG CCAACCGGAC GGAGGACGAC 
ATCCTCCTCC GCGACGAGCT CGACCGGTGG GCGGCCGAGT ACCCGGACAG GCTCAAGGTG 
TGGTACGTCA TCGACCAGGT AAAGCGCCCG GAGGAGGGGT GGAAGTACAG CGTTGGGTTC 
GTCACGGAGG CCGTCCTGCG GGAGCACGTT CCGGAAGGTG GGGACGACAC GCTGGCCCTT 
GCCTGCGGAC CACCGCCGAT GATCCAGTTC GCCATCTCGC CCAACTTGGA GAAGATGAAG 
TACGACATGG CCAATTCTTT CGTCGTGTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292785.1
CDS	150..2882
Translation	MAAVEPRQFGRLEPGSSPVRVATNGAKAYPPPASHLPRRADSPVRGCSFPPLVSPPRRLDDASDDEDEEQEDWRELYGSHLQLEVEPAVQDARDEGTADAWIERNPCLVRLTGKHPLNCEPPLARLMHHGFITPAPLHYVRNHGAVPRGDWATWTVEVTGLVRRPARLTMEELARDFPAVEIPVTLACAGNRRKEQNMVQQTVGFNWGAAGVSTSVWRGARLRDVLRRCGIVPRKGGALNVCFEGAEDLPGGGGSKYGTSVTREWALDPSRDIMLAYMQNGEPLLPDHGFPVRVIIPGCIGGRMVKWLKRIIVTPAESDNYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITTPGHDEILPINSITTQRGYTMKGYAYSGGGKKVTRVEVTLDGGETWLVCHLDHPEKPNKYGKYWCWCFWSVEVEVLDLLGAKEIAVRAWDQSLNTQPEKLIWNLMGMMNNCWFKVKVNVCRPHKGEIGLVFEHPTQPGNQPGGWMARQKHLETAEAAAPGLKRSTSTPFMNTTDVGKQFTMSEVRKHASQESAWIVVHGHVYDCTKFLKDHPGGADSILINAGTDCTEEFDAIHSDKAKALLDTYRIGELITTGTGYSSDNSVHGGSVLSHLAPIREAVRAPALSNPREKIHCRLVGKKELSRDVRLFRFSLPSPDQVLGLPIGKHIFVCASIEGKLCMRAYTPTSMVDEIGHFDLLVKVYFKNEHPKFPNGGLMTQYLDSLPVGSYIDVKGPLGHVEYTGRGSFVINGKQRHASRLAMICGGSGITPMYQIIQAVLREQPEDHTEMHLVYANRTEDDILLRDELDRWAAEYPDRLKVWYVIDQVKRPEEGWKYSVGFVTEAVLREHVPEGGDDTLALACGPPPMIQFAISPNLEKMKYDMANSFVVF

Target ORF information:

RefSeq Version	NM_001305856.1
Organism	Zea mays
Definition	Zea mays nitrate reductase [NADH]-like (LOC542278), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305856.1

ORF Insert Sequence:

ATGGCCGCTG TGGAGCCCCG GCAGTTCGGG CGTCTGGAGC CGGGCTCCTC GCCAGTGCGC 
GTGGCCACGA ACGGCGCCAA GGCGTACCCT CCTCCGGCAA GCCACCTCCC GCGGCGCGCT 
GACTCCCCTG TCCGCGGCTG CAGCTTCCCT CCCCTCGTCT CCCCGCCGCG CAGGCTGGAC 
GACGCGTCGG ACGACGAGGA CGAGGAGCAG GAGGACTGGC GAGAGCTGTA CGGCTCGCAC 
CTGCAGCTGG AGGTGGAGCC CGCGGTGCAG GACGCGCGCG ACGAAGGCAC CGCGGACGCG 
TGGATCGAGC GGAACCCGTG CCTGGTGAGG CTGACGGGGA AGCACCCGCT CAACTGCGAG 
CCGCCGCTGG CGCGGCTGAT GCACCACGGC TTCATCACCC CGGCGCCGCT GCACTACGTG 
CGCAACCACG GCGCCGTGCC GCGGGGGGAC TGGGCGACGT GGACCGTGGA GGTGACGGGC 
CTCGTGAGGC GGCCCGCGCG GCTCACCATG GAGGAGCTGG CGCGCGACTT CCCCGCCGTG 
GAGATCCCCG TGACGCTGGC GTGCGCGGGC AACCGGCGCA AGGAGCAGAA CATGGTGCAG 
CAGACGGTGG GCTTCAACTG GGGCGCCGCC GGCGTGTCCA CGTCCGTGTG GCGCGGCGCG 
CGCCTCCGCG ACGTGCTCCG CCGCTGCGGC ATCGTGCCCC GCAAGGGCGG CGCCCTCAAC 
GTGTGCTTCG AGGGCGCCGA GGACCTCCCC GGCGGCGGGG GATCCAAGTA CGGCACCAGC 
GTCACGCGCG AGTGGGCCCT GGACCCGTCG CGGGACATCA TGCTCGCCTA CATGCAGAAC 
GGCGAGCCGC TGCTGCCGGA CCACGGCTTC CCCGTGCGCG TCATCATCCC CGGCTGCATC 
GGTGGCCGCA TGGTCAAGTG GCTCAAGCGC ATCATCGTCA CCCCCGCCGA GTCCGACAAT 
TACTACCATT TCAAGGACAA CCGCGTCCTG CCGTCGCACG TCGACGCCGA GCTCGCCAAC 
GCAGAAGCGT GGTGGTACAA GCCGGAGTAC ATCATCAACG AGCTGAACAT AAACTCGGTG 
ATAACGACGC CGGGGCACGA CGAGATCCTG CCCATCAACA GCATTACCAC ACAGCGCGGC 
TACACCATGA AAGGATACGC CTACTCCGGC GGCGGCAAGA AGGTGACGCG GGTGGAGGTG 
ACGCTGGACG GCGGCGAGAC ATGGCTGGTG TGCCACCTCG ACCACCCGGA GAAGCCCAAC 
AAGTACGGCA AGTACTGGTG CTGGTGCTTC TGGTCCGTCG AGGTGGAGGT CCTCGACCTG 
CTCGGCGCCA AGGAGATCGC CGTGCGCGCA TGGGACCAGT CGCTCAACAC CCAGCCCGAG 
AAGCTCATAT GGAACCTCAT GGGGATGATG AACAACTGCT GGTTCAAGGT GAAGGTGAAC 
GTGTGCCGTC CGCACAAGGG CGAGATCGGG CTGGTGTTCG AGCACCCGAC GCAGCCCGGC 
AACCAGCCCG GCGGGTGGAT GGCGCGGCAG AAGCACCTGG AGACGGCGGA GGCGGCCGCG 
CCGGGCCTCA AGCGCAGCAC GTCCACGCCG TTCATGAACA CCACCGACGT CGGCAAGCAG 
TTCACCATGT CCGAGGTGCG CAAGCACGCG TCGCAGGAGT CGGCGTGGAT CGTCGTGCAC 
GGCCACGTCT ACGACTGCAC CAAGTTCCTC AAGGACCACC CGGGCGGCGC CGACAGCATC 
CTCATCAACG CCGGTACCGA CTGCACCGAG GAGTTCGACG CCATCCACTC CGACAAGGCC 
AAGGCGCTCC TCGACACCTA CCGCATCGGC GAGCTCATCA CCACGGGCAC CGGCTACAGC 
TCCGACAACT CCGTCCACGG CGGCTCCGTC CTGTCGCACC TCGCGCCCAT CCGCGAGGCC 
GTCAGGGCTC CCGCGCTCTC CAACCCGCGC GAAAAGATCC ACTGCCGCCT CGTCGGCAAG 
AAGGAGCTGT CCCGCGACGT CCGCCTCTTC CGCTTCTCGC TGCCGTCGCC CGACCAGGTG 
CTCGGCCTCC CCATCGGCAA GCACATCTTC GTCTGCGCCA GTATTGAAGG GAAGCTGTGT 
ATGCGGGCGT ACACGCCCAC GAGCATGGTG GACGAGATCG GCCACTTCGA CCTCCTCGTC 
AAGGTCTACT TCAAGAACGA GCACCCCAAG TTCCCCAACG GCGGCCTCAT GACCCAGTAC 
CTCGACTCGC TTCCCGTCGG CTCCTACATC GACGTCAAGG GCCCGCTTGG CCACGTGGAG 
TACACCGGCC GCGGCAGCTT CGTCATCAAC GGCAAGCAGC GCCATGCGAG CCGCCTCGCC 
ATGATCTGCG GCGGAAGTGG GATCACGCCC ATGTACCAGA TCATCCAGGC GGTGCTGCGC 
GAGCAGCCGG AGGACCACAC GGAGATGCAC CTCGTGTACG CCAACCGGAC GGAGGACGAC 
ATCCTCCTCC GCGACGAGCT CGACCGGTGG GCGGCCGAGT ACCCGGACAG GCTCAAGGTG 
TGGTACGTCA TCGACCAGGT AAAGCGCCCG GAGGAGGGGT GGAAGTACAG CGTTGGGTTC 
GTCACGGAGG CCGTCCTGCG GGAGCACGTT CCGGAAGGTG GGGACGACAC GCTGGCCCTT 
GCCTGCGGAC CACCGCCGAT GATCCAGTTC GCCATCTCGC CCAACTTGGA GAAGATGAAG 
TACGACATGG CCAATTCTTT CGTCGTGTTC TAA