dmrt2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dmrt2b
Entrez Gene ID	558970
Full Name	doublesex and mab-3 related transcription factor 2b
General protein information	Preferred Names doublesex and mab-3 related transcription factor 2b Names doublesex and mab-3 related transcription factor 2b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001079976.1	NP_001073445.1	doublesex and mab-3 related transcription factor 2b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos.
Lu C, Wu J, Xiong S, Zhang X, Zhang J, Mei J
Gene62549-54(2017 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Expression profiling of the sex-related gene Dmrt1 in adults of the Lusitanian toadfish Halobatrachus didactylus (Bloch and Schneider, 1801).
Ubeda-Manzanaro M, Merlo MA, Ortiz-Delgado JB, Rebordinos L, Sarasquete C
Gene535(2)255-65(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

fish-specific duplicated dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway and maintains the common function for left-right asymmetry establishment
Title: Fish-specific duplicated dmrt2b contributes to a divergent function through Hedgehog pathway and maintains left-right asymmetry establishment function.

Cloning, expression and synteny analysis of a duplicated copy of zebrafish Dmrt2a gene, the Dmrt2b which is fish specific, whereas Dmrt2a exists in all vertebrates.
Title: Fish specific duplication of Dmrt2: characterization of zebrafish Dmrt2b.

The following dmrt2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dmrt2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36885	NM_001079976.1 Latest version!	Danio rerio doublesex and mab-3 related transcription factor 2b (dmrt2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dmrt2b ( NM_001079976.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073445.1 Danio rerio doublesex and mab-3 related transcription factor 2b (dmrt2b), mRNA.

Price & Availability↑

CloneID	ODa36885
Clone ID Related Accession (Same CDS sequence)	NM_001079976.1
Accession Version	NM_001079976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Danio rerio(zebrafish)
Product	doublesex and mab-3 related transcription factor 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ307066.1. On Mar 11, 2007 this sequence version replaced XM_682264.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ307066.1, BC163825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGTCCACTA AAGCGGATAG GGGCGCACAG GTAGATGCGA CCCACCTAGA GTGCCTGAAA 
GCGGAGTGGG TTGCAGACTG CGCCGGACGG GAGGTCATGG GCACGGATGA AGCTCCCGCG 
GCGCGCAGGC TCAGCCGCAG CCCGAAGTGC GCTCGCTGCA GGAACCACGG CGTCGTGTCG 
CGCCTGAAGG GCCACAAGCG CCTGTGTCGC TGGCGGGACT GTCAGTGCGC CAACTGCCTG 
CTGGTCGTGG AGAGGCAGCG AGTGATGGCA GCTCAAGTCG CCCTACGGAG ACAGCAGGCC 
ACAGAGGGTA AGAAAGGTCA GAAAAACAGC GCTGTTCTCA GACGCACAGC TTACCAGCGC 
TACACGAGGG CACCGAGTCT CTTGGCAAAG AGTATTCTAG AGGGCTACAA GCCACCAGTA 
CCGGAAGACT GGCCGAAACG ACTCCACCAA CCCCCCGTGA GTGTTCGGAT GAGGAAGAGA 
AGAGCGTTTG CTGATAAAGA GCTGGAGACG GTGATGCTGG AGAGAGAGCT GAGGCAGAGA 
GAGATAGAGG AGCTGCCCTC TCTCCTCCTG CAGGCTGTGG TGCCCTCTGC CCCCTCCCCG 
TATTTCTTCC CGCTGTCAGA CCCAATCATG CCCACATTTG TGCCTGTTTC CAAAAACAGC 
CCACCTTTGA CTGACTGTGA TCTCCGTCAA CACGGGCTCA AATCTAAAAC TCTAGACACA 
CTGACAGATG ACGTCTCTCT GAGAGTGTGC AAGAATTGGG ACATTTTCAG TTCCATTCAG 
TCCCCATTGC AATGTTACAA CAGCACAAGC CCAGGTGTCA ACTTGGTTAA AAACACACCG 
GACAGAAGCT TTTCTGATTT GTATGTCAAA CCAAAATTTG CAGTTGCTGA CATCATGTCA 
AAGCAAGACA TTCCTCCAAT ACTGAACATG GCGGATGTGT TGATACATGC AAAATCGCCA 
CACAAACGCT GCAGGTCATT GGGACATGAG GCCTCTGTAC GTTTGGACTG TGTGGAGGGT 
GCTAGTAAAG ACCCCGTCAA AAAAGCTGTA ACACGGCCAT TACCCTTTTC TGTCGAGGCA 
CTGCTCATGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073445.1
CDS	1..1095
Translation	MSTKADRGAQVDATHLECLKAEWVADCAGREVMGTDEAPAARRLSRSPKCARCRNHGVVSRLKGHKRLCRWRDCQCANCLLVVERQRVMAAQVALRRQQATEGKKGQKNSAVLRRTAYQRYTRAPSLLAKSILEGYKPPVPEDWPKRLHQPPVSVRMRKRRAFADKELETVMLERELRQREIEELPSLLLQAVVPSAPSPYFFPLSDPIMPTFVPVSKNSPPLTDCDLRQHGLKSKTLDTLTDDVSLRVCKNWDIFSSIQSPLQCYNSTSPGVNLVKNTPDRSFSDLYVKPKFAVADIMSKQDIPPILNMADVLIHAKSPHKRCRSLGHEASVRLDCVEGASKDPVKKAVTRPLPFSVEALLMR

Target ORF information:

RefSeq Version	NM_001079976.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio doublesex and mab-3 related transcription factor 2b (dmrt2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079976.1

ORF Insert Sequence:

ATGTCCACTA AAGCGGATAG GGGCGCACAG GTAGATGCGA CCCACCTAGA GTGCCTGAAA 
GCGGAGTGGG TTGCAGACTG CGCCGGACGG GAGGTCATGG GCACGGATGA AGCTCCCGCG 
GCGCGCAGGC TCAGCCGCAG CCCGAAGTGC GCTCGCTGCA GGAACCACGG CGTCGTGTCG 
CGCCTGAAGG GCCACAAGCG CCTGTGTCGC TGGCGGGACT GTCAGTGCGC CAACTGCCTG 
CTGGTCGTGG AGAGGCAGCG AGTGATGGCA GCTCAAGTCG CCCTACGGAG ACAGCAGGCC 
ACAGAGGGTA AGAAAGGTCA GAAAAACAGC GCTGTTCTCA GACGCACAGC TTACCAGCGC 
TACACGAGGG CACCGAGTCT CTTGGCAAAG AGTATTCTAG AGGGCTACAA GCCACCAGTA 
CCGGAAGACT GGCCGAAACG ACTCCACCAA CCCCCCGTGA GTGTTCGGAT GAGGAAGAGA 
AGAGCGTTTG CTGATAAAGA GCTGGAGACG GTGATGCTGG AGAGAGAGCT GAGGCAGAGA 
GAGATAGAGG AGCTGCCCTC TCTCCTCCTG CAGGCTGTGG TGCCCTCTGC CCCCTCCCCG 
TATTTCTTCC CGCTGTCAGA CCCAATCATG CCCACATTTG TGCCTGTTTC CAAAAACAGC 
CCACCTTTGA CTGACTGTGA TCTCCGTCAA CACGGGCTCA AATCTAAAAC TCTAGACACA 
CTGACAGATG ACGTCTCTCT GAGAGTGTGC AAGAATTGGG ACATTTTCAG TTCCATTCAG 
TCCCCATTGC AATGTTACAA CAGCACAAGC CCAGGTGTCA ACTTGGTTAA AAACACACCG 
GACAGAAGCT TTTCTGATTT GTATGTCAAA CCAAAATTTG CAGTTGCTGA CATCATGTCA 
AAGCAAGACA TTCCTCCAAT ACTGAACATG GCGGATGTGT TGATACATGC AAAATCGCCA 
CACAAACGCT GCAGGTCATT GGGACATGAG GCCTCTGTAC GTTTGGACTG TGTGGAGGGT 
GCTAGTAAAG ACCCCGTCAA AAAAGCTGTA ACACGGCCAT TACCCTTTTC TGTCGAGGCA 
CTGCTCATGA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos.
Gene62549-54(2017 Aug)
Lu C,Wu J,Xiong S,Zhang X,Zhang J,Mei J

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Expression profiling of the sex-related gene Dmrt1 in adults of the Lusitanian toadfish Halobatrachus didactylus (Bloch and Schneider, 1801).
Gene535(2)255-65(2014 Feb)
Ubeda-Manzanaro M,Merlo MA,Ortiz-Delgado JB,Rebordinos L,Sarasquete C