BIK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BIK
Entrez Gene ID	638
Full Name	BCL2 interacting killer
Synonyms	BIP1,BP4,NBK
General protein information	Preferred Names BCL2 interacting killer Names bcl-2-interacting killer BCL2-interacting killer (apoptosis-inducing) apoptosis inducer NBK apoptosis-inducing NBK natural born killer
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.2
Summary	The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011].
Disorder MIM:	603392

mRNA and Protein(s)

mRNA	Protein	Name
NM_001197.5	NP_001188.1	bcl-2-interacting killer
NM_001197.4	NP_001188.1	bcl-2-interacting killer

Pathways from BioSystems

Homologies

Homo sapiens (human)	BIK	NP_001188.1
Mus musculus (house mouse)	Bik	NP_031572.1
Pan troglodytes (chimpanzee)	BIK	XP_525617.3
	BIK	XP_001108040.1
Rattus norvegicus (Norway rat)	Bik	NP_446156.1

Gene Ontology
Bibliography

Related articles in PubMed

Blocking Bcl-2 resolves IL-13-mediated mucous cell hyperplasia in a Bik-dependent manner.
Chand HS, Mebratu YA, Kuehl PJ, Tesfaigzi Y
The Journal of allergy and clinical immunology140(5)1456-1459.e9(2017 Nov)

Autophagy regulatory molecule, TMEM74, interacts with BIK and inhibits BIK-induced apoptosis.
Sun Y, Li Q, Zhang J, Chen Z, He Q, Liu X, Zhao N, Yin A, Huang H, He M, Cao L, Wang L
Cellular signalling3634-41(2017 Aug)

Biphasic ROS production, p53 and BIK dictate the mode of cell death in response to DNA damage in colon cancer cells.
Kutuk O, Aytan N, Karakas B, Kurt AG, Acikbas U, Temel SG, Basaga H
PloS one12(8)e0182809(2017)

LncRNA H19 confers chemoresistance in ER?-positive breast cancer through epigenetic silencing of the pro-apoptotic gene BIK.
Si X, Zang R, Zhang E, Liu Y, Shi X, Zhang E, Shao L, Li A, Yang N, Han X, Pan B, Zhang Z, Sun L, Sun Y
Oncotarget7(49)81452-81462(2016 Dec)

The pro-apoptotic paradox: the BH3-only protein Bcl-2 interacting killer (Bik) is prognostic for unfavorable outcomes in breast cancer.
Pandya V, Glubrecht D, Vos L, Hanson J, Damaraju S, Mackey J, Hugh J, Goping IS
Oncotarget7(22)33272-85(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BIK significantly contributes to DNA damage-induced mitochondrial apoptosis in HCT-116 wt cells upstream of the second peak of ROS production, BAX and BAK activation, cytochrome c release and caspase activation.
Title: Biphasic ROS production, p53 and BIK dictate the mode of cell death in response to DNA damage in colon cancer cells.

These studies suggest a link between Bik-mediated caspase activation and cleavage of viral proteins.
Title: Bik Mediates Caspase-Dependent Cleavage of Viral Proteins to Promote Influenza A Virus Infection.

Suggest complex mechanism of tumor promotion in Bik high breast tumors.
Title: The pro-apoptotic paradox: the BH3-only protein Bcl-2 interacting killer (Bik) is prognostic for unfavorable outcomes in breast cancer.

our data demonstrated that suppression of BIK in ER-positive MCF-7 cells prevents the cytotoxic effect of TAM and favors a more aggressive phenotype, due to the molecular change of different pathways
Title: Involvement of multiple cellular pathways in regulating resistance to tamoxifen in BIK-suppressed MCF-7 cells.

HCV RNA replication and release were significantly suppressed in BIK-depleted cells and over-expression of the RNA-dependent RNA polymerase, NS5B, was able to induce BIK expression
Title: NS5B induces up-regulation of the BH3-only protein, BIK, essential for the hepatitis C virus RNA replication and viral release.

The following BIK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BIK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55968	NM_001197.5 Latest version!	Homo sapiens BCL2 interacting killer (BIK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu55968	NM_001197.4	Homo sapiens BCL2 interacting killer (BIK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BIK ( NM_001197.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188.1 Homo sapiens BCL2 interacting killer (BIK), mRNA.

Price & Availability↑

CloneID	OHu55968
Clone ID Related Accession (Same CDS sequence)	NM_001197.5 , NM_001197.4
Accession Version	NM_001197.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	bcl-2-interacting killer
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP329261.1, BC001599.1 and U34584.1. On Nov 22, 2018 this sequence version replaced NM_001197.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.81286.1, SRR1163657.62147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216115.3/ ENSP00000216115.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGAAG TAAGACCCCT CTCCAGAGAC ATCTTGATGG AGACCCTCCT GTATGAGCAG 
CTCCTGGAAC CCCCGACCAT GGAGGTTCTT GGCATGACTG ACTCTGAAGA GGACCTGGAC 
CCTATGGAGG ACTTCGATTC TTTGGAATGC ATGGAGGGCA GTGACGCATT GGCCCTGCGG 
CTGGCCTGCA TCGGGGACGA GATGGACGTG AGCCTCAGGG CCCCGCGCCT GGCCCAGCTC 
TCCGAGGTGG CCATGCACAG CCTGGGTCTG GCTTTCATCT ACGACCAGAC TGAGGACATC 
AGGGATGTTC TTAGAAGTTT CATGGACGGT TTCACCACAC TTAAGGAGAA CATAATGAGG 
TTCTGGAGAT CCCCGAACCC CGGGTCCTGG GTGTCCTGCG AACAGGTGCT GCTGGCGCTG 
CTGCTGCTGC TGGCGCTGCT GCTGCCGCTG CTCAGCGGGG GCCTGCACCT GCTGCTCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188.1
CDS	62..544
Translation	MSEVRPLSRDILMETLLYEQLLEPPTMEVLGMTDSEEDLDPMEDFDSLECMEGSDALALRLACIGDEMDVSLRAPRLAQLSEVAMHSLGLAFIYDQTEDIRDVLRSFMDGFTTLKENIMRFWRSPNPGSWVSCEQVLLALLLLLALLLPLLSGGLHLLLK

Target ORF information:

RefSeq Version	NM_001197.5
Organism	Homo sapiens(human)
Definition	Homo sapiens BCL2 interacting killer (BIK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197.5

ORF Insert Sequence:

ATGTCTGAAG TAAGACCCCT CTCCAGAGAC ATCTTGATGG AGACCCTCCT GTATGAGCAG 
CTCCTGGAAC CCCCGACCAT GGAGGTTCTT GGCATGACTG ACTCTGAAGA GGACCTGGAC 
CCTATGGAGG ACTTCGATTC TTTGGAATGC ATGGAGGGCA GTGACGCATT GGCCCTGCGG 
CTGGCCTGCA TCGGGGACGA GATGGACGTG AGCCTCAGGG CCCCGCGCCT GGCCCAGCTC 
TCCGAGGTGG CCATGCACAG CCTGGGTCTG GCTTTCATCT ACGACCAGAC TGAGGACATC 
AGGGATGTTC TTAGAAGTTT CATGGACGGT TTCACCACAC TTAAGGAGAA CATAATGAGG 
TTCTGGAGAT CCCCGAACCC CGGGTCCTGG GTGTCCTGCG AACAGGTGCT GCTGGCGCTG 
CTGCTGCTGC TGGCGCTGCT GCTGCCGCTG CTCAGCGGGG GCCTGCACCT GCTGCTCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BIK ( NM_001197.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188.1 Homo sapiens BCL2 interacting killer (BIK), mRNA.

Price & Availability↑

CloneID	OHu55968
Clone ID Related Accession (Same CDS sequence)	NM_001197.5 , NM_001197.4
Accession Version	NM_001197.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-30
Organism	Homo sapiens(human)
Product	bcl-2-interacting killer
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP329261.1, BC001599.1 and U34584.1. On Sep 24, 2011 this sequence version replaced NM_001197.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.81286.1, SRR1163657.62147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAAG TAAGACCCCT CTCCAGAGAC ATCTTGATGG AGACCCTCCT GTATGAGCAG 
CTCCTGGAAC CCCCGACCAT GGAGGTTCTT GGCATGACTG ACTCTGAAGA GGACCTGGAC 
CCTATGGAGG ACTTCGATTC TTTGGAATGC ATGGAGGGCA GTGACGCATT GGCCCTGCGG 
CTGGCCTGCA TCGGGGACGA GATGGACGTG AGCCTCAGGG CCCCGCGCCT GGCCCAGCTC 
TCCGAGGTGG CCATGCACAG CCTGGGTCTG GCTTTCATCT ACGACCAGAC TGAGGACATC 
AGGGATGTTC TTAGAAGTTT CATGGACGGT TTCACCACAC TTAAGGAGAA CATAATGAGG 
TTCTGGAGAT CCCCGAACCC CGGGTCCTGG GTGTCCTGCG AACAGGTGCT GCTGGCGCTG 
CTGCTGCTGC TGGCGCTGCT GCTGCCGCTG CTCAGCGGGG GCCTGCACCT GCTGCTCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188.1
CDS	64..546
Translation	MSEVRPLSRDILMETLLYEQLLEPPTMEVLGMTDSEEDLDPMEDFDSLECMEGSDALALRLACIGDEMDVSLRAPRLAQLSEVAMHSLGLAFIYDQTEDIRDVLRSFMDGFTTLKENIMRFWRSPNPGSWVSCEQVLLALLLLLALLLPLLSGGLHLLLK

Target ORF information:

RefSeq Version	NM_001197.4
Organism	Homo sapiens(human)
Definition	Homo sapiens BCL2 interacting killer (BIK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197.4

ORF Insert Sequence:

ATGTCTGAAG TAAGACCCCT CTCCAGAGAC ATCTTGATGG AGACCCTCCT GTATGAGCAG 
CTCCTGGAAC CCCCGACCAT GGAGGTTCTT GGCATGACTG ACTCTGAAGA GGACCTGGAC 
CCTATGGAGG ACTTCGATTC TTTGGAATGC ATGGAGGGCA GTGACGCATT GGCCCTGCGG 
CTGGCCTGCA TCGGGGACGA GATGGACGTG AGCCTCAGGG CCCCGCGCCT GGCCCAGCTC 
TCCGAGGTGG CCATGCACAG CCTGGGTCTG GCTTTCATCT ACGACCAGAC TGAGGACATC 
AGGGATGTTC TTAGAAGTTT CATGGACGGT TTCACCACAC TTAAGGAGAA CATAATGAGG 
TTCTGGAGAT CCCCGAACCC CGGGTCCTGG GTGTCCTGCG AACAGGTGCT GCTGGCGCTG 
CTGCTGCTGC TGGCGCTGCT GCTGCCGCTG CTCAGCGGGG GCCTGCACCT GCTGCTCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Blocking Bcl-2 resolves IL-13-mediated mucous cell hyperplasia in a Bik-dependent manner.
The Journal of allergy and clinical immunology140(5)1456-1459.e9(2017 Nov)
Chand HS,Mebratu YA,Kuehl PJ,Tesfaigzi Y

Autophagy regulatory molecule, TMEM74, interacts with BIK and inhibits BIK-induced apoptosis.
Cellular signalling3634-41(2017 Aug)
Sun Y,Li Q,Zhang J,Chen Z,He Q,Liu X,Zhao N,Yin A,Huang H,He M,Cao L,Wang L

Biphasic ROS production, p53 and BIK dictate the mode of cell death in response to DNA damage in colon cancer cells.
PloS one12(8)e0182809(2017)
Kutuk O,Aytan N,Karakas B,Kurt AG,Acikbas U,Temel SG,Basaga H

LncRNA H19 confers chemoresistance in ER?-positive breast cancer through epigenetic silencing of the pro-apoptotic gene BIK.
Oncotarget7(49)81452-81462(2016 Dec)
Si X,Zang R,Zhang E,Liu Y,Shi X,Zhang E,Shao L,Li A,Yang N,Han X,Pan B,Zhang Z,Sun L,Sun Y

The pro-apoptotic paradox: the BH3-only protein Bcl-2 interacting killer (Bik) is prognostic for unfavorable outcomes in breast cancer.
Oncotarget7(22)33272-85(2016 May)
Pandya V,Glubrecht D,Vos L,Hanson J,Damaraju S,Mackey J,Hugh J,Goping IS

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BIK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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