APOE cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	APOE
Entrez Gene ID	100009337
Full Name	apolipoprotein E
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082643.1	NP_001076112.1	apolipoprotein E precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Efficient creation of an APOE knockout rabbit.
Ji D, Zhao G, Songstad A, Cui X, Weinstein EJ
Transgenic research24(2)227-35(2015 Apr)

Identification of differentially expressed proteins in atherosclerotic aorta and effect of vitamin E.
Kaga E, Karademir B, Baykal AT, Ozer NK
Journal of proteomics92260-73(2013 Oct)

LCAT cholesterol esterification is associated with the increase of ApoE/ApoA-I ratio during atherosclerosis progression in rabbit.
Carlucci A, Cigliano L, Maresca B, Spagnuolo MS, Di Salvo G, Calabr? R, Abrescia P
Journal of physiology and biochemistry68(4)541-53(2012 Dec)

Apolipoprotein-E forms dimers in human frontal cortex and hippocampus.
Elliott DA, Halliday GM, Garner B
BMC neuroscience1123(2010 Feb)

ApoE mimetic peptide reduces plasma lipid hydroperoxide content with a concomitant increase in HDL paraoxonase activity.
Datta G, Chaddha M, Handattu SP, Palgunachari MN, Nayyar G, Garber DW, Gupta H, White CR, Anantharamaiah GM
Advances in experimental medicine and biology6601-4(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we report the efficient creation of an APOE knockout rabbit by using zinc finger nucleases. The knockout rabbits had drastically elevated cholesterol and moderately increased triglyceride levels, mimicking symptoms in human heart disease.
Title: Efficient creation of an APOE knockout rabbit.

These differentially expressed proteins associated with key mechanisms involved in atherosclerosis and signaling mechanisms related with vitamin E.
Title: Identification of differentially expressed proteins in atherosclerotic aorta and effect of vitamin E.

The molar ratio ApoE/ApoA-I is negatively correlated with the enzyme activity, and positively correlated with increases in the intima-media thickness of common carotid wall and cardiac dysfunction signs.
Title: LCAT cholesterol esterification is associated with the increase of ApoE/ApoA-I ratio during atherosclerosis progression in rabbit.

The identification of disulphide-linked apoE dimers in cortical and hippocampal tissues represents a distinct structural difference between the apoE3 and apoE4 isoforms that may have functional consequences.
Title: Apolipoprotein-E forms dimers in human frontal cortex and hippocampus.

ApoE mimetic peptide reduces plasma lipid hydroperoxide content with a concomitant increase in HDL paraoxonase activity
Title: ApoE mimetic peptide reduces plasma lipid hydroperoxide content with a concomitant increase in HDL paraoxonase activity.

The following APOE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APOE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb00035	NM_001082643.1 Latest version!	Oryctolagus cuniculus apolipoprotein E (APOE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APOE ( NM_001082643.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001076112.1 Oryctolagus cuniculus apolipoprotein E (APOE), mRNA.

Price & Availability↑

CloneID	OOb00035
Clone ID Related Accession (Same CDS sequence)	NM_001082643.1
Accession Version	NM_001082643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Oryctolagus cuniculus(rabbit)
Product	apolipoprotein E precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M36603.1. ##Evidence-Data-START## Transcript exon combination :: M36603.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00013637, SAMN00013641 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGTGT GGTGGGCTGT GTTGGCGGCG GCGATCCTGG CAGGATGCCG GGCCCAGACG 
GAGCAGGAGG TGGAGGTGCC GGAGCAGGCC AGGTGGAAGG CCGGCCAGCC CTGGGAGCTG 
GCCCTGGGCC GCTTCTGGGA TTACCTGCGC TGGGTACAGT CGCTGTCTGA TCAGGTGCAG 
GAGGAGCTGC TCAGCTCGCA GGTCACCCAG GAACTGACGA TGCTGATGGA AGAAACCATG 
AAGGAGGTGA AGGCCTACAA GTCGGAGCTG GAGGAGCAGC TGAGCCCCAT GGCGCAGGAG 
CACCGGGCGC GCCTGTCCAA GGAGCTGCAG GTGGCAGGCG CGCTGGAGGC CGACATGGAG 
GACGTCTGCA ACCGCCTGGC GCAGTACCGC GGCGAGGCGC AGGCCATGCT GGGCCAGAGC 
ACCGAGGAGC TCGCGCGCGC GTTCTCCTCG CACCTGCGCA AGCTGCGCAA GCGGCTGCTG 
CGCGACGCCG AGGACCTGCA GAAGCGCATG GCGGTGTACG GCGCCGGGGC CCGCGAAGGC 
GCCGAGCGCG GCGTGAGCGC CGTCCGCGAG CGCCTCGGGT CGCGGTTGGA GCGGGGCCGC 
CTGCGGGTCG CCACCGTAGG CACCCTGGCC GGCCGGCCGC TGCGGGAGCG CGCGCAGGCC 
TGGGGCGAGC GGCTGCGCGG GCACCTGGAG GAGGTGGGCA GCCGCGCGCG CGACCGCCTG 
AACGAGGTGC GCGAGCAGGT GGAGGAGGTG CGGGTGAAGG TGGAGGAGCA GGCGCCGCAG 
ATGCGCCTGC AGGCCGAGGC CTTCCAGGCC CGCCTCAAGA GCTGGTTCGA GCCGCTGGTG 
GAAGACATGC AGCGCCAGTG GGCCGGGCTG GTGGAGAAGC TGCAGGCCGC CATGCCCAGC 
AAGGCGCCCG CGGCGGCGCC CATCGAGAAC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076112.1
CDS	59..994
Translation	MKVWWAVLAAAILAGCRAQTEQEVEVPEQARWKAGQPWELALGRFWDYLRWVQSLSDQVQEELLSSQVTQELTMLMEETMKEVKAYKSELEEQLSPMAQEHRARLSKELQVAGALEADMEDVCNRLAQYRGEAQAMLGQSTEELARAFSSHLRKLRKRLLRDAEDLQKRMAVYGAGAREGAERGVSAVRERLGSRLERGRLRVATVGTLAGRPLRERAQAWGERLRGHLEEVGSRARDRLNEVREQVEEVRVKVEEQAPQMRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKLQAAMPSKAPAAAPIENQ

Target ORF information:

RefSeq Version	NM_001082643.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus apolipoprotein E (APOE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082643.1

ORF Insert Sequence:

ATGAAGGTGT GGTGGGCTGT GTTGGCGGCG GCGATCCTGG CAGGATGCCG GGCCCAGACG 
GAGCAGGAGG TGGAGGTGCC GGAGCAGGCC AGGTGGAAGG CCGGCCAGCC CTGGGAGCTG 
GCCCTGGGCC GCTTCTGGGA TTACCTGCGC TGGGTACAGT CGCTGTCTGA TCAGGTGCAG 
GAGGAGCTGC TCAGCTCGCA GGTCACCCAG GAACTGACGA TGCTGATGGA AGAAACCATG 
AAGGAGGTGA AGGCCTACAA GTCGGAGCTG GAGGAGCAGC TGAGCCCCAT GGCGCAGGAG 
CACCGGGCGC GCCTGTCCAA GGAGCTGCAG GTGGCAGGCG CGCTGGAGGC CGACATGGAG 
GACGTCTGCA ACCGCCTGGC GCAGTACCGC GGCGAGGCGC AGGCCATGCT GGGCCAGAGC 
ACCGAGGAGC TCGCGCGCGC GTTCTCCTCG CACCTGCGCA AGCTGCGCAA GCGGCTGCTG 
CGCGACGCCG AGGACCTGCA GAAGCGCATG GCGGTGTACG GCGCCGGGGC CCGCGAAGGC 
GCCGAGCGCG GCGTGAGCGC CGTCCGCGAG CGCCTCGGGT CGCGGTTGGA GCGGGGCCGC 
CTGCGGGTCG CCACCGTAGG CACCCTGGCC GGCCGGCCGC TGCGGGAGCG CGCGCAGGCC 
TGGGGCGAGC GGCTGCGCGG GCACCTGGAG GAGGTGGGCA GCCGCGCGCG CGACCGCCTG 
AACGAGGTGC GCGAGCAGGT GGAGGAGGTG CGGGTGAAGG TGGAGGAGCA GGCGCCGCAG 
ATGCGCCTGC AGGCCGAGGC CTTCCAGGCC CGCCTCAAGA GCTGGTTCGA GCCGCTGGTG 
GAAGACATGC AGCGCCAGTG GGCCGGGCTG GTGGAGAAGC TGCAGGCCGC CATGCCCAGC 
AAGGCGCCCG CGGCGGCGCC CATCGAGAAC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient creation of an APOE knockout rabbit.
Transgenic research24(2)227-35(2015 Apr)
Ji D,Zhao G,Songstad A,Cui X,Weinstein EJ

Identification of differentially expressed proteins in atherosclerotic aorta and effect of vitamin E.
Journal of proteomics92260-73(2013 Oct)
Kaga E,Karademir B,Baykal AT,Ozer NK

LCAT cholesterol esterification is associated with the increase of ApoE/ApoA-I ratio during atherosclerosis progression in rabbit.
Journal of physiology and biochemistry68(4)541-53(2012 Dec)
Carlucci A,Cigliano L,Maresca B,Spagnuolo MS,Di Salvo G,Calabr? R,Abrescia P

Apolipoprotein-E forms dimers in human frontal cortex and hippocampus.
BMC neuroscience1123(2010 Feb)
Elliott DA,Halliday GM,Garner B

ApoE mimetic peptide reduces plasma lipid hydroperoxide content with a concomitant increase in HDL paraoxonase activity.
Advances in experimental medicine and biology6601-4(2010)
Datta G,Chaddha M,Handattu SP,Palgunachari MN,Nayyar G,Garber DW,Gupta H,White CR,Anantharamaiah GM

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APOE cDNA ORF clone, Oryctolagus cuniculus(rabbit)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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