PLCH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCH1
Entrez Gene ID	23007
Full Name	phospholipase C eta 1
Synonyms	PLCL3
General protein information	Preferred Names phospholipase C eta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 PLC eta 1 PLC-L3 phosphoinositide phospholipase C-eta-1 phospholipase C-eta1a phospholipase C-eta1b phospholipase C-like 3 phospholipase C-like protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q25.31
Summary	PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009].
Disorder MIM:	612835

mRNA and Protein(s)

mRNA	Protein	Name
NM_001349251.1	NP_001336180.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform d
NM_001130961.1	NP_001124433.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
NM_001130961.2	NP_001124433.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
NM_001130960.2	NP_001124432.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform a
XM_011512560.3	XP_011510862.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_011512561.2	XP_011510863.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_017005923.1	XP_016861412.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
NM_001349250.1	NP_001336179.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
NM_001349250.2	NP_001336179.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
XM_005247239.1	XP_005247296.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
NM_001349251.2	NP_001336180.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform d
XM_005247238.1	XP_005247295.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_011512562.3	XP_011510864.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_011512565.2	XP_011510867.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_017005926.1	XP_016861415.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_011512562.2	XP_011510864.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
NM_001130960.1	NP_001124432.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform a
NM_001349252.2	NP_001336181.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform e
NM_014996.3	NP_055811.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform b
NM_001349252.1	NP_001336181.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform e
XM_011512560.2	XP_011510862.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
XM_011512564.2	XP_011510866.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
NM_014996.4	NP_055811.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform b
XM_017005925.1	XP_016861414.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
XM_011512566.2	XP_011510868.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
XM_017005927.1	XP_016861416.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
XM_011512567.1	XP_011510869.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLCH1	XP_001149022.1
	LOC707243	XP_002808381.1
Bos taurus (cattle)	PLCH1	XP_002685035.3
Rattus norvegicus (Norway rat)	Plch1	NP_001178636.1
Xenopus tropicalis (tropical clawed frog)	plch1	XP_002933304.2
Homo sapiens (human)	PLCH1	NP_001124432.1
Gallus gallus (chicken)	PLCH1	XP_004943620.1
Danio rerio (zebrafish)	LOC566479	XP_694841.5
Mus musculus (house mouse)	Plch1	NP_899014.2
Canis lupus familiaris (dog)	PLCH1	XP_005634651.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression profile of PLC?, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation.
Tavalaee M, Nasr-Esfahani MH
Andrology4(5)850-6(2016 Sep)

A canonical EF-loop directs Ca(2+) -sensitivity in phospholipase C-?2.
Popovics P, Lu J, Nadia Kamil L, Morgan K, Millar RP, Schmid R, Blindauer CA, Stewart AJ
Journal of cellular biochemistry115(3)557-65(2014 Mar)

Association between polymorphisms in COMT, PLCH1, and CYP17A1, and non-small-cell lung cancer risk in Chinese nonsmokers.
Zhang Y, Hua S, Zhang A, Kong X, Jiang C, Deng D, Wenlong B
Clinical lung cancer14(1)45-9(2013 Jan)

Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
Lauc G, Huffman JE, Pu?i? M, Zgaga L, Adamczyk B, Mu?ini? A, Novokmet M, Pola?ek O, Gornik O, Kri?ti? J, Keser T, Vitart V, Scheijen B, Uh HW, Molokhia M, Patrick AL, McKeigue P, Kol?i? I, Luki? IK, Swann O, van Leeuwen FN, Ruhaak LR, Houwing-Duistermaat JJ, Slagboom PE, Beekman M, de Craen AJ, Deelder AM, Zeng Q, Wang W, Hastie ND, Gyllensten U, Wilson JF, Wuhrer M, Wright AF, Rudd PM, Hayward C, Aulchenko Y, Campbell H, Rudan I
PLoS genetics9(1)e1003225(2013)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCzeta, PAWP, and TR-KIT were low in globozoospermic individuals
Title: Expression profile of PLC?, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation.

A molecular model of the PLCeta2 EF-hand domain, constructed based upon the structure of calmodulin, suggested both EF-loops may participate in Ca(2+) -binding.
Title: A canonical EF-loop directs Ca(2+) -sensitivity in phospholipase C-?2.

The PLCH1 rs181696 single-nucleotide polymorphism was strongly associated with an increased risk of squamous cell carcinoma.
Title: Association between polymorphisms in COMT, PLCH1, and CYP17A1, and non-small-cell lung cancer risk in Chinese nonsmokers.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following PLCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu93905	XM_017005927.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu30734	NM_001349250.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu30734	NM_001130961.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65294	NM_001349252.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu65294	XM_011512567.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu100999	NM_001349251.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu37790	NM_014996.4 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu93903	XM_017005923.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu93904	XM_017005925.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu31414	NM_001130960.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37789	XM_011512562.3 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37789	XM_011512560.3 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37789	XM_005247238.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37789	XM_011512561.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37790	XM_011512564.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37791	XM_005247239.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu65293	XM_011512566.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu65293	XM_017005926.1 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu65293	XM_011512565.2 Latest version!	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu30734	NM_001130961.1	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu30734	NM_001349250.1	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu100999	NM_001349251.1	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu37789	XM_011512562.2	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu31414	NM_001130960.1	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu65294	NM_001349252.1	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu37789	XM_011512560.2	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu37790	NM_014996.3	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCH1 ( XM_017005927.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861416.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X12, mRNA.

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CloneID	OHu93905
Clone ID Related Accession (Same CDS sequence)	XM_017005927.1
Accession Version	XM_017005927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGGGTCAC TCCGGAGAGG AACTGTATGG 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861416.1
CDS	291..3356
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLGSLRRGTVWR

Target ORF information:

RefSeq Version	XM_017005927.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005927.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGGGTCAC TCCGGAGAGG AACTGTATGG 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001130961.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124433.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu30734
Clone ID Related Accession (Same CDS sequence)	NM_001130961.1 , NM_001130961.2 , NM_001349250.1 , NM_001349250.2
Accession Version	NM_001130961.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA206803.1, AK096620.1, BC113950.1 and AY691170.1. Transcript Variant: This variant (3), as well as variant 4, encodes isoform c. ##Evidence-Data-START## Transcript exon combination :: AY691170.1, SRR1803612.145534.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124433.1
CDS	278..3286
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	NM_001130961.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130961.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001130961.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124433.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu30734
Clone ID Related Accession (Same CDS sequence)	NM_001130961.1 , NM_001130961.2 , NM_001349250.1 , NM_001349250.2
Accession Version	NM_001130961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132006.2, DA206803.1, AK096620.1, BC113950.1, AY691170.1 and AC012022.13. On Jun 2, 2019 this sequence version replaced NM_001130961.1. Transcript Variant: This variant (3), as well as variant 4, encodes isoform c. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.108287.1, AY691170.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124433.1
CDS	305..3313
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	NM_001130961.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130961.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001349250.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336179.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu30734
Clone ID Related Accession (Same CDS sequence)	NM_001130961.1 , NM_001130961.2 , NM_001349250.1 , NM_001349250.2
Accession Version	NM_001349250.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132006.2 and AC012022.13. On Mar 9, 2017 this sequence version replaced XM_017005929.1. Transcript Variant: This variant (4), as well as variant 3, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.204602.1, SRR1803614.68166.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336179.1
CDS	278..3286
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	NM_001349250.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349250.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001349250.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336179.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu30734
Clone ID Related Accession (Same CDS sequence)	NM_001130961.1 , NM_001130961.2 , NM_001349250.1 , NM_001349250.2
Accession Version	NM_001349250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132006.2 and AC012022.13. On Jun 2, 2019 this sequence version replaced NM_001349250.1. Transcript Variant: This variant (4), as well as variant 3, encodes isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.204602.1, SRR1803615.1322.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336179.1
CDS	305..3313
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	NM_001349250.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349250.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGTA 
CAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001349252.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336181.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu65294
Clone ID Related Accession (Same CDS sequence)	NM_001349252.2 , NM_001349252.1 , XM_011512567.1
Accession Version	NM_001349252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104472.14, AC132006.2 and AC012022.13. On Jun 2, 2019 this sequence version replaced NM_001349252.1. Transcript Variant: This variant (6) encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.145125.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGTACAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336181.1
CDS	273..3317
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	NM_001349252.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349252.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGTACAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001349252.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336181.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu65294
Clone ID Related Accession (Same CDS sequence)	NM_001349252.2 , NM_001349252.1 , XM_011512567.1
Accession Version	NM_001349252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform e
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104472.14, AC132006.2 and AC012022.13. On Mar 9, 2017 this sequence version replaced XM_017005928.1. Transcript Variant: This variant (6) encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.145125.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGTACAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336181.1
CDS	291..3335
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	NM_001349252.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349252.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGTACAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512567.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510869.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X13, mRNA.

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CloneID	OHu65294
Clone ID Related Accession (Same CDS sequence)	NM_001349252.2 , NM_001349252.1 , XM_011512567.1
Accession Version	XM_011512567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGTACAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510869.1
CDS	291..3335
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLVQI

Target ORF information:

RefSeq Version	XM_011512567.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512567.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGTACAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001349251.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336180.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 5, mRNA.

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CloneID	OHu100999
Clone ID Related Accession (Same CDS sequence)	NM_001349251.1 , NM_001349251.2
Accession Version	NM_001349251.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104472.14, AC132006.2 and AC012022.13. On Mar 9, 2017 this sequence version replaced XM_017005924.1. Transcript Variant: This variant (5) encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.11765.1, SRR1803612.229258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGAAGATA AAGATGGCAG AAGAAAAGGG 
AAAGCAAGTA TAAAAGATCC ACATTTTCTA AATTTCAACA AAAAGTTATC ATCCTCCTCC 
AGTGCTCTGC TCCACAAAGA TACCAGCCAA GGGGACACCA TTGTATCTAC TGCCCACATG 
TCAGTCACAG GAGAACAGCT GGGCATGTCA AGTCCTAGGG GTGGGAGAAC CACATCAAAT 
GCCACAAGCA ATTGCCAGGA AAACCCCTGT CCCAGCAAGT CTCTCTCCCC AAAGCAGCAT 
TTGGCTCCCG ATCCTGTAGT TAACCCCACA CAAGATCTGC ATGGTGTGAA AATCAAGGAA 
AAGGGTAATC CTGAGGACTT TGTGGAAGGG AAAAGCATCT TGTCAGGAAG CGTCCTTTCT 
CATAGCAACC TAGAAATTAA GAACCTGGAA GGTAATAGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCTT TGTCAGACGT CTCCATGCTC TGTTCTGACA TACCTGACCT ACATTCAACT 
GCAATTCTGC AGGAGAGTGT AATTTCCCAT CTTATTGACA ATGTCACTTT AACAAATGAG 
AATGAGCCGG GCAGTTCCAT CTCAGCCCTG ATTGGCCAGT TTGATGAGAC CAACAATCAG 
GCTCTCACAG TTGTTTCTCA TCTTCATAAT ACCAGTGTGA TGTCAGGCCA TTGTCCCTTG 
CCTAGCCTGG GCCTAAAAAT GCCCATCAAG CATGGTTTTT GCAAGGGAAA ATCCAAGTCT 
TCCTTCCTGT GCTCATCTCC GGAGCTGATA GCACTCTCGA GTTCTGAGAC CACCAAACAT 
GCAACGAACA CAGTTTATGA AACTACCTGC ACTCCCATCT CTAAAACCAA ACCAGATGAT 
GACCTTTCTA GTAAGGCCAA GACAGCGGCC TTAGAAAGCA ACCTGCCTGG ATCCCCTAAT 
ACTTCTCGTG GCTGGTTACC AAAAAGTCCT ACCAAGGGAG AAGACTGGGA AACACTGAAG 
AGCTGCAGCC CTGCCTCTTC CCCTGATTTG ACCCTGGAGG ATGTAATAGC TGATCCCACT 
CTCTGTTTCA ATTCTGGGGA GAGCAGCCTT GTGGAAATTG ATGGAGAATC AGAAAATCTT 
TCTCTAACAA CCTGTGAATA TAGGAGAGAG GGCACAAGTC AACTTGCTTC TCCTTTAAAA 
CTCAAGTACA ATCAGGGTGT GGTAGAACAC TTTCAAAGAG GTTTGAGAAA CGGCTACTGT 
AAAGAGACCC TCCGCCCTTC TGTCCCTGAA ATATTCAACA ATATTCAAGA TGTCAAAACT 
CAAAGTATTT CTTATCTAGC CTATCAGGGT GCTGGCTTTG TGCATAATCA TTTCTCAGAT 
TCAGATGCAA AAATGTTCCA GACCTGTGTG CCCCAGCAAT CTAGTGCTCA AGATATGCAT 
GTCCCTGTAC CCAAGCAGTT GGCACATCTT CCTTTGCCTG CTCTGAAACT GCCTAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACTTAACA TCAGAGGACA TTGCCTGCAA TTTTGAGAGC 
AAGTACCAGT GTATTAGTAA GAGTTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGCGTG 
ACTGTGAAGA CAAAGTCGTT AGAGCCTATA GATGCCCTGA CCGAGCAGCT TCGGAAGCTT 
GTGTCCTTTG ACCAGGAAGA CAACTGCCAA GTGCTATATT CAAAGCAGGA TGCCAATCAG 
CTCCCCCGGG CACTGGTCAG GAAGTTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCTAGTCGTG CCAAGGAGAA ACAGGAAGCC AACAAGCAGA AAGTTCCAAA CCCCAGCAAT 
GGGGCAGGAG TGGTTCTTAG AAACAAACCC TCAGCACCCA CCCCTGCAGT GAATCGCCAC 
TCCACCGGCT CCTACATCGC AGGCTACCTG AAGAACACGA AAGGGGGTGG CCTTGAAGGC 
CGGGGCATCC CAGAGGGGGC ATGCACGGCT CTTCACTATG GCCACGTTGA CCAGTTTTGT 
TCAGATAATT CTGTTTTGCA GACTGAGCCA AGCAGTGATG ATAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336180.1
CDS	291..5345
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_001349251.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349251.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGAAGATA AAGATGGCAG AAGAAAAGGG 
AAAGCAAGTA TAAAAGATCC ACATTTTCTA AATTTCAACA AAAAGTTATC ATCCTCCTCC 
AGTGCTCTGC TCCACAAAGA TACCAGCCAA GGGGACACCA TTGTATCTAC TGCCCACATG 
TCAGTCACAG GAGAACAGCT GGGCATGTCA AGTCCTAGGG GTGGGAGAAC CACATCAAAT 
GCCACAAGCA ATTGCCAGGA AAACCCCTGT CCCAGCAAGT CTCTCTCCCC AAAGCAGCAT 
TTGGCTCCCG ATCCTGTAGT TAACCCCACA CAAGATCTGC ATGGTGTGAA AATCAAGGAA 
AAGGGTAATC CTGAGGACTT TGTGGAAGGG AAAAGCATCT TGTCAGGAAG CGTCCTTTCT 
CATAGCAACC TAGAAATTAA GAACCTGGAA GGTAATAGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCTT TGTCAGACGT CTCCATGCTC TGTTCTGACA TACCTGACCT ACATTCAACT 
GCAATTCTGC AGGAGAGTGT AATTTCCCAT CTTATTGACA ATGTCACTTT AACAAATGAG 
AATGAGCCGG GCAGTTCCAT CTCAGCCCTG ATTGGCCAGT TTGATGAGAC CAACAATCAG 
GCTCTCACAG TTGTTTCTCA TCTTCATAAT ACCAGTGTGA TGTCAGGCCA TTGTCCCTTG 
CCTAGCCTGG GCCTAAAAAT GCCCATCAAG CATGGTTTTT GCAAGGGAAA ATCCAAGTCT 
TCCTTCCTGT GCTCATCTCC GGAGCTGATA GCACTCTCGA GTTCTGAGAC CACCAAACAT 
GCAACGAACA CAGTTTATGA AACTACCTGC ACTCCCATCT CTAAAACCAA ACCAGATGAT 
GACCTTTCTA GTAAGGCCAA GACAGCGGCC TTAGAAAGCA ACCTGCCTGG ATCCCCTAAT 
ACTTCTCGTG GCTGGTTACC AAAAAGTCCT ACCAAGGGAG AAGACTGGGA AACACTGAAG 
AGCTGCAGCC CTGCCTCTTC CCCTGATTTG ACCCTGGAGG ATGTAATAGC TGATCCCACT 
CTCTGTTTCA ATTCTGGGGA GAGCAGCCTT GTGGAAATTG ATGGAGAATC AGAAAATCTT 
TCTCTAACAA CCTGTGAATA TAGGAGAGAG GGCACAAGTC AACTTGCTTC TCCTTTAAAA 
CTCAAGTACA ATCAGGGTGT GGTAGAACAC TTTCAAAGAG GTTTGAGAAA CGGCTACTGT 
AAAGAGACCC TCCGCCCTTC TGTCCCTGAA ATATTCAACA ATATTCAAGA TGTCAAAACT 
CAAAGTATTT CTTATCTAGC CTATCAGGGT GCTGGCTTTG TGCATAATCA TTTCTCAGAT 
TCAGATGCAA AAATGTTCCA GACCTGTGTG CCCCAGCAAT CTAGTGCTCA AGATATGCAT 
GTCCCTGTAC CCAAGCAGTT GGCACATCTT CCTTTGCCTG CTCTGAAACT GCCTAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACTTAACA TCAGAGGACA TTGCCTGCAA TTTTGAGAGC 
AAGTACCAGT GTATTAGTAA GAGTTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGCGTG 
ACTGTGAAGA CAAAGTCGTT AGAGCCTATA GATGCCCTGA CCGAGCAGCT TCGGAAGCTT 
GTGTCCTTTG ACCAGGAAGA CAACTGCCAA GTGCTATATT CAAAGCAGGA TGCCAATCAG 
CTCCCCCGGG CACTGGTCAG GAAGTTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCTAGTCGTG CCAAGGAGAA ACAGGAAGCC AACAAGCAGA AAGTTCCAAA CCCCAGCAAT 
GGGGCAGGAG TGGTTCTTAG AAACAAACCC TCAGCACCCA CCCCTGCAGT GAATCGCCAC 
TCCACCGGCT CCTACATCGC AGGCTACCTG AAGAACACGA AAGGGGGTGG CCTTGAAGGC 
CGGGGCATCC CAGAGGGGGC ATGCACGGCT CTTCACTATG GCCACGTTGA CCAGTTTTGT 
TCAGATAATT CTGTTTTGCA GACTGAGCCA AGCAGTGATG ATAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001349251.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336180.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 5, mRNA.

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CloneID	OHu100999
Clone ID Related Accession (Same CDS sequence)	NM_001349251.1 , NM_001349251.2
Accession Version	NM_001349251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104472.14, AC132006.2 and AC012022.13. On Jun 2, 2019 this sequence version replaced NM_001349251.1. Transcript Variant: This variant (5) encodes isoform d. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.11765.1, SRR1803612.229258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGAAGATA AAGATGGCAG AAGAAAAGGG 
AAAGCAAGTA TAAAAGATCC ACATTTTCTA AATTTCAACA AAAAGTTATC ATCCTCCTCC 
AGTGCTCTGC TCCACAAAGA TACCAGCCAA GGGGACACCA TTGTATCTAC TGCCCACATG 
TCAGTCACAG GAGAACAGCT GGGCATGTCA AGTCCTAGGG GTGGGAGAAC CACATCAAAT 
GCCACAAGCA ATTGCCAGGA AAACCCCTGT CCCAGCAAGT CTCTCTCCCC AAAGCAGCAT 
TTGGCTCCCG ATCCTGTAGT TAACCCCACA CAAGATCTGC ATGGTGTGAA AATCAAGGAA 
AAGGGTAATC CTGAGGACTT TGTGGAAGGG AAAAGCATCT TGTCAGGAAG CGTCCTTTCT 
CATAGCAACC TAGAAATTAA GAACCTGGAA GGTAATAGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCTT TGTCAGACGT CTCCATGCTC TGTTCTGACA TACCTGACCT ACATTCAACT 
GCAATTCTGC AGGAGAGTGT AATTTCCCAT CTTATTGACA ATGTCACTTT AACAAATGAG 
AATGAGCCGG GCAGTTCCAT CTCAGCCCTG ATTGGCCAGT TTGATGAGAC CAACAATCAG 
GCTCTCACAG TTGTTTCTCA TCTTCATAAT ACCAGTGTGA TGTCAGGCCA TTGTCCCTTG 
CCTAGCCTGG GCCTAAAAAT GCCCATCAAG CATGGTTTTT GCAAGGGAAA ATCCAAGTCT 
TCCTTCCTGT GCTCATCTCC GGAGCTGATA GCACTCTCGA GTTCTGAGAC CACCAAACAT 
GCAACGAACA CAGTTTATGA AACTACCTGC ACTCCCATCT CTAAAACCAA ACCAGATGAT 
GACCTTTCTA GTAAGGCCAA GACAGCGGCC TTAGAAAGCA ACCTGCCTGG ATCCCCTAAT 
ACTTCTCGTG GCTGGTTACC AAAAAGTCCT ACCAAGGGAG AAGACTGGGA AACACTGAAG 
AGCTGCAGCC CTGCCTCTTC CCCTGATTTG ACCCTGGAGG ATGTAATAGC TGATCCCACT 
CTCTGTTTCA ATTCTGGGGA GAGCAGCCTT GTGGAAATTG ATGGAGAATC AGAAAATCTT 
TCTCTAACAA CCTGTGAATA TAGGAGAGAG GGCACAAGTC AACTTGCTTC TCCTTTAAAA 
CTCAAGTACA ATCAGGGTGT GGTAGAACAC TTTCAAAGAG GTTTGAGAAA CGGCTACTGT 
AAAGAGACCC TCCGCCCTTC TGTCCCTGAA ATATTCAACA ATATTCAAGA TGTCAAAACT 
CAAAGTATTT CTTATCTAGC CTATCAGGGT GCTGGCTTTG TGCATAATCA TTTCTCAGAT 
TCAGATGCAA AAATGTTCCA GACCTGTGTG CCCCAGCAAT CTAGTGCTCA AGATATGCAT 
GTCCCTGTAC CCAAGCAGTT GGCACATCTT CCTTTGCCTG CTCTGAAACT GCCTAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACTTAACA TCAGAGGACA TTGCCTGCAA TTTTGAGAGC 
AAGTACCAGT GTATTAGTAA GAGTTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGCGTG 
ACTGTGAAGA CAAAGTCGTT AGAGCCTATA GATGCCCTGA CCGAGCAGCT TCGGAAGCTT 
GTGTCCTTTG ACCAGGAAGA CAACTGCCAA GTGCTATATT CAAAGCAGGA TGCCAATCAG 
CTCCCCCGGG CACTGGTCAG GAAGTTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCTAGTCGTG CCAAGGAGAA ACAGGAAGCC AACAAGCAGA AAGTTCCAAA CCCCAGCAAT 
GGGGCAGGAG TGGTTCTTAG AAACAAACCC TCAGCACCCA CCCCTGCAGT GAATCGCCAC 
TCCACCGGCT CCTACATCGC AGGCTACCTG AAGAACACGA AAGGGGGTGG CCTTGAAGGC 
CGGGGCATCC CAGAGGGGGC ATGCACGGCT CTTCACTATG GCCACGTTGA CCAGTTTTGT 
TCAGATAATT CTGTTTTGCA GACTGAGCCA AGCAGTGATG ATAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336180.1
CDS	273..5327
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_001349251.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349251.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGAAGATA AAGATGGCAG AAGAAAAGGG 
AAAGCAAGTA TAAAAGATCC ACATTTTCTA AATTTCAACA AAAAGTTATC ATCCTCCTCC 
AGTGCTCTGC TCCACAAAGA TACCAGCCAA GGGGACACCA TTGTATCTAC TGCCCACATG 
TCAGTCACAG GAGAACAGCT GGGCATGTCA AGTCCTAGGG GTGGGAGAAC CACATCAAAT 
GCCACAAGCA ATTGCCAGGA AAACCCCTGT CCCAGCAAGT CTCTCTCCCC AAAGCAGCAT 
TTGGCTCCCG ATCCTGTAGT TAACCCCACA CAAGATCTGC ATGGTGTGAA AATCAAGGAA 
AAGGGTAATC CTGAGGACTT TGTGGAAGGG AAAAGCATCT TGTCAGGAAG CGTCCTTTCT 
CATAGCAACC TAGAAATTAA GAACCTGGAA GGTAATAGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCTT TGTCAGACGT CTCCATGCTC TGTTCTGACA TACCTGACCT ACATTCAACT 
GCAATTCTGC AGGAGAGTGT AATTTCCCAT CTTATTGACA ATGTCACTTT AACAAATGAG 
AATGAGCCGG GCAGTTCCAT CTCAGCCCTG ATTGGCCAGT TTGATGAGAC CAACAATCAG 
GCTCTCACAG TTGTTTCTCA TCTTCATAAT ACCAGTGTGA TGTCAGGCCA TTGTCCCTTG 
CCTAGCCTGG GCCTAAAAAT GCCCATCAAG CATGGTTTTT GCAAGGGAAA ATCCAAGTCT 
TCCTTCCTGT GCTCATCTCC GGAGCTGATA GCACTCTCGA GTTCTGAGAC CACCAAACAT 
GCAACGAACA CAGTTTATGA AACTACCTGC ACTCCCATCT CTAAAACCAA ACCAGATGAT 
GACCTTTCTA GTAAGGCCAA GACAGCGGCC TTAGAAAGCA ACCTGCCTGG ATCCCCTAAT 
ACTTCTCGTG GCTGGTTACC AAAAAGTCCT ACCAAGGGAG AAGACTGGGA AACACTGAAG 
AGCTGCAGCC CTGCCTCTTC CCCTGATTTG ACCCTGGAGG ATGTAATAGC TGATCCCACT 
CTCTGTTTCA ATTCTGGGGA GAGCAGCCTT GTGGAAATTG ATGGAGAATC AGAAAATCTT 
TCTCTAACAA CCTGTGAATA TAGGAGAGAG GGCACAAGTC AACTTGCTTC TCCTTTAAAA 
CTCAAGTACA ATCAGGGTGT GGTAGAACAC TTTCAAAGAG GTTTGAGAAA CGGCTACTGT 
AAAGAGACCC TCCGCCCTTC TGTCCCTGAA ATATTCAACA ATATTCAAGA TGTCAAAACT 
CAAAGTATTT CTTATCTAGC CTATCAGGGT GCTGGCTTTG TGCATAATCA TTTCTCAGAT 
TCAGATGCAA AAATGTTCCA GACCTGTGTG CCCCAGCAAT CTAGTGCTCA AGATATGCAT 
GTCCCTGTAC CCAAGCAGTT GGCACATCTT CCTTTGCCTG CTCTGAAACT GCCTAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACTTAACA TCAGAGGACA TTGCCTGCAA TTTTGAGAGC 
AAGTACCAGT GTATTAGTAA GAGTTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGCGTG 
ACTGTGAAGA CAAAGTCGTT AGAGCCTATA GATGCCCTGA CCGAGCAGCT TCGGAAGCTT 
GTGTCCTTTG ACCAGGAAGA CAACTGCCAA GTGCTATATT CAAAGCAGGA TGCCAATCAG 
CTCCCCCGGG CACTGGTCAG GAAGTTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCTAGTCGTG CCAAGGAGAA ACAGGAAGCC AACAAGCAGA AAGTTCCAAA CCCCAGCAAT 
GGGGCAGGAG TGGTTCTTAG AAACAAACCC TCAGCACCCA CCCCTGCAGT GAATCGCCAC 
TCCACCGGCT CCTACATCGC AGGCTACCTG AAGAACACGA AAGGGGGTGG CCTTGAAGGC 
CGGGGCATCC CAGAGGGGGC ATGCACGGCT CTTCACTATG GCCACGTTGA CCAGTTTTGT 
TCAGATAATT CTGTTTTGCA GACTGAGCCA AGCAGTGATG ATAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_014996.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055811.2 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2, mRNA.

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CloneID	OHu37790
Clone ID Related Accession (Same CDS sequence)	NM_014996.3 , XM_011512564.2 , NM_014996.4
Accession Version	NM_014996.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104472.14, AC132006.2 and AC012022.13. On or before Oct 31, 2019 this sequence version replaced XM_011512564.2, NM_014996.3. Transcript Variant: This variant (2) encodes isoform b. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.154719.1, SRR7346977.2631208.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055811.2
CDS	273..5330
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_014996.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014996.4

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_017005923.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861412.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

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CloneID	OHu93903
Clone ID Related Accession (Same CDS sequence)	XM_017005923.1
Accession Version	XM_017005923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGAAGATA AAGATGGCAG AAGAAAAGGG 
AAAGCAAGTA TAAAAGATCC ACATTTTCTA AATTTCAACA AAAAGTTATC ATCCTCCTCC 
AGTGCTCTGC TCCACAAAGA TACCAGCCAA GGGGACACCA TTGTATCTAC TGCCCACATG 
TCAGTCACAG GAGAACAGCT GGGCATGTCA AGTCCTAGGG GTGGGAGAAC CACATCAAAT 
GCCACAAGCA ATTGCCAGGA AAACCCCTGT CCCAGCAAGT CTCTCTCCCC AAAGCAGCAT 
TTGGCTCCCG ATCCTGTAGT TAACCCCACA CAAGATCTGC ATGGTGTGAA AATCAAGGAA 
AAGGGTAATC CTGAGGACTT TGTGGAAGGG AAAAGCATCT TGTCAGGAAG CGTCCTTTCT 
CATAGCAACC TAGAAATTAA GAACCTGGAA GGTAATAGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCTT TGTCAGACGT CTCCATGCTC TGTTCTGACA TACCTGACCT ACATTCAACT 
GCAATTCTGC AGGAGAGTGT AATTTCCCAT CTTATTGACA ATGTCACTTT AACAAATGAG 
AATGAGCCGG GCAGTTCCAT CTCAGCCCTG ATTGGCCAGT TTGATGAGAC CAACAATCAG 
GCTCTCACAG TTGTTTCTCA TCTTCATAAT ACCAGTGTGA TGTCAGGCCA TTGTCCCTTG 
CCTAGCCTGG GCCTAAAAAT GCCCATCAAG CATGGTTTTT GCAAGGGAAA ATCCAAGTCT 
TCCTTCCTGT GCTCATCTCC GGAGCTGATA GCACTCTCGA GTTCTGAGAC CACCAAACAT 
GCAACGAACA CAGTTTATGA AACTACCTGC ACTCCCATCT CTAAAACCAA ACCAGATGAT 
GACCTTTCTA GTAAGGCCAA GACAGCGGCC TTAGAAAGCA ACCTGCCTGG ATCCCCTAAT 
ACTTCTCGTG GCTGGTTACC AAAAAGTCCT ACCAAGGGAG AAGACTGGGA AACACTGAAG 
AGCTGCAGCC CTGCCTCTTC CCCTGATTTG ACCCTGGAGG ATGTAATAGC TGATCCCACT 
CTCTGTTTCA ATTCTGGGGA GAGCAGCCTT GTGGAAATTG ATGGAGAATC AGAAAATCTT 
TCTCTAACAA CCTGTGAATA TAGGAGAGAG GGCACAAGTC AACTTGCTTC TCCTTTAAAA 
CTCAAGTACA ATCAGGGTGT GGTAGAACAC TTTCAAAGAG GTTTGAGAAA CGGCTACTGT 
AAAGAGACCC TCCGCCCTTC TGTCCCTGAA ATATTCAACA ATATTCAAGA TGTCAAAACT 
CAAAGTATTT CTTATCTAGC CTATCAGGGT GCTGGCTTTG TGCATAATCA TTTCTCAGAT 
TCAGATGCAA AAATGTTCCA GACCTGTGTG CCCCAGCAAT CTAGTGCTCA AGATATGCAT 
GTCCCTGTAC CCAAGCAGTT GGCACATCTT CCTTTGCCTG CTCTGAAACT GCCTAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACTTAACA TCAGAGGACA TTGCCTGCAA TTTTGAGAGC 
AAGTACCAGT GTATTAGTAA GAGTTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGCGTG 
ACTGTGAAGA CAAAGTCGTT AGAGCCTATA GATGCCCTGA CCGAGCAGCT TCGGAAGCTT 
GTGTCCTTTG ACCAGGAAGA CAACTGCCAA GTGCTATATT CAAAGCAGGA TGCCAATCAG 
CTCCCCCGGG CACTGGTCAG GAAGTTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCTAGTCGTG CCAAGGAGAA ACAGGAAGCC AACAAGCAGA AAGTTCCAAA CCCCAGCAAT 
GGGGCAGGAG TGGTTCTTAG AAACAAACCC TCAGCACCCA CCCCTGCAGT GAATCGCCAC 
TCCACCGGCT CCTACATCGC AGGCTACCTG AAGAACACGA AAGGGGGTGG CCTTGAAGGC 
CGGGGCATCC CAGAGGGGGC ATGCACGGCT CTTCACTATG GCCACGTTGA CCAGTTTTGT 
TCAGATAATT CTGTTTTGCA GACTGAGCCA AGCAGTGATG ATAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861412.1
CDS	291..5405
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017005923.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005923.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGAAGATA AAGATGGCAG AAGAAAAGGG 
AAAGCAAGTA TAAAAGATCC ACATTTTCTA AATTTCAACA AAAAGTTATC ATCCTCCTCC 
AGTGCTCTGC TCCACAAAGA TACCAGCCAA GGGGACACCA TTGTATCTAC TGCCCACATG 
TCAGTCACAG GAGAACAGCT GGGCATGTCA AGTCCTAGGG GTGGGAGAAC CACATCAAAT 
GCCACAAGCA ATTGCCAGGA AAACCCCTGT CCCAGCAAGT CTCTCTCCCC AAAGCAGCAT 
TTGGCTCCCG ATCCTGTAGT TAACCCCACA CAAGATCTGC ATGGTGTGAA AATCAAGGAA 
AAGGGTAATC CTGAGGACTT TGTGGAAGGG AAAAGCATCT TGTCAGGAAG CGTCCTTTCT 
CATAGCAACC TAGAAATTAA GAACCTGGAA GGTAATAGGG GTAAGGGCCG AGCTGCAACA 
TCCTTTTCTT TGTCAGACGT CTCCATGCTC TGTTCTGACA TACCTGACCT ACATTCAACT 
GCAATTCTGC AGGAGAGTGT AATTTCCCAT CTTATTGACA ATGTCACTTT AACAAATGAG 
AATGAGCCGG GCAGTTCCAT CTCAGCCCTG ATTGGCCAGT TTGATGAGAC CAACAATCAG 
GCTCTCACAG TTGTTTCTCA TCTTCATAAT ACCAGTGTGA TGTCAGGCCA TTGTCCCTTG 
CCTAGCCTGG GCCTAAAAAT GCCCATCAAG CATGGTTTTT GCAAGGGAAA ATCCAAGTCT 
TCCTTCCTGT GCTCATCTCC GGAGCTGATA GCACTCTCGA GTTCTGAGAC CACCAAACAT 
GCAACGAACA CAGTTTATGA AACTACCTGC ACTCCCATCT CTAAAACCAA ACCAGATGAT 
GACCTTTCTA GTAAGGCCAA GACAGCGGCC TTAGAAAGCA ACCTGCCTGG ATCCCCTAAT 
ACTTCTCGTG GCTGGTTACC AAAAAGTCCT ACCAAGGGAG AAGACTGGGA AACACTGAAG 
AGCTGCAGCC CTGCCTCTTC CCCTGATTTG ACCCTGGAGG ATGTAATAGC TGATCCCACT 
CTCTGTTTCA ATTCTGGGGA GAGCAGCCTT GTGGAAATTG ATGGAGAATC AGAAAATCTT 
TCTCTAACAA CCTGTGAATA TAGGAGAGAG GGCACAAGTC AACTTGCTTC TCCTTTAAAA 
CTCAAGTACA ATCAGGGTGT GGTAGAACAC TTTCAAAGAG GTTTGAGAAA CGGCTACTGT 
AAAGAGACCC TCCGCCCTTC TGTCCCTGAA ATATTCAACA ATATTCAAGA TGTCAAAACT 
CAAAGTATTT CTTATCTAGC CTATCAGGGT GCTGGCTTTG TGCATAATCA TTTCTCAGAT 
TCAGATGCAA AAATGTTCCA GACCTGTGTG CCCCAGCAAT CTAGTGCTCA AGATATGCAT 
GTCCCTGTAC CCAAGCAGTT GGCACATCTT CCTTTGCCTG CTCTGAAACT GCCTAGTCCT 
TGCAAATCCA AAAGTCTGGG GGACTTAACA TCAGAGGACA TTGCCTGCAA TTTTGAGAGC 
AAGTACCAGT GTATTAGTAA GAGTTTTGTT ACAACTGGCA TTAGAGACAA GAAGGGCGTG 
ACTGTGAAGA CAAAGTCGTT AGAGCCTATA GATGCCCTGA CCGAGCAGCT TCGGAAGCTT 
GTGTCCTTTG ACCAGGAAGA CAACTGCCAA GTGCTATATT CAAAGCAGGA TGCCAATCAG 
CTCCCCCGGG CACTGGTCAG GAAGTTGTCA TCCAGAAGTC AGAGCAGAGT GCGCAATATT 
GCTAGTCGTG CCAAGGAGAA ACAGGAAGCC AACAAGCAGA AAGTTCCAAA CCCCAGCAAT 
GGGGCAGGAG TGGTTCTTAG AAACAAACCC TCAGCACCCA CCCCTGCAGT GAATCGCCAC 
TCCACCGGCT CCTACATCGC AGGCTACCTG AAGAACACGA AAGGGGGTGG CCTTGAAGGC 
CGGGGCATCC CAGAGGGGGC ATGCACGGCT CTTCACTATG GCCACGTTGA CCAGTTTTGT 
TCAGATAATT CTGTTTTGCA GACTGAGCCA AGCAGTGATG ATAAACCAGA AATTTATTTT 
CTTTTGAGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_017005925.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861414.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

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CloneID	OHu93904
Clone ID Related Accession (Same CDS sequence)	XM_017005925.1
Accession Version	XM_017005925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAAAACTAC AAAATCACGG TCTAAATCTT ACAGTACTGA TGATGAGGAA 
GACACACAGC AGAGTACTGG CAAGGAGGGT GGCCAGCTGT ACAGATTGGG TCGCCGAAGG 
AAAACCATGA AGCTCTGCCG AGAACTCTCT GATTTGGTTG TGTACACAAA CTCCGTGGCC 
GCTCAGGACA TTGTGGATGA CGGAACCACA GGAAATGTGT TATCATTCAG TGAAACAAGA 
GCACATCAGG TTGTTCAGCA AAAATCAGAG CAGTTCATGA TTTATAATCA AAAGCAACTC 
ACGAGGATTT ACCCCTCTGC CTACCGCATT GATTCCAGTA ACTTCAACCC TCTCCCCTAC 
TGGAACGCAG GCTGCCAGCT AGTGGCACTG AATTATCAAT CTGAAGGACG AATGATGCAG 
TTAAACCGAG CCAAATTCAA GGCAAATGGC AATTGTGGCT ATGTCCTCAA ACCCCAGCAA 
ATGTGCAAAG GTACTTTCAA CCCTTTCTCT GGTGACCCTC TTCCTGCCAA CCCCAAAAAG 
CAGCTCATCC TGAAAGTTAT CAGTGGACAG CAACTCCCCA AACCTCCAGA CTCCATGTTT 
GGAGATCGAG GCGAGATCAT TGACCCTTTT GTTGAAGTTG AAATTATTGG ATTGCCAGTA 
GATTGTTGTA AAGATCAAAC CCGTGTGGTA GATGACAATG GATTTAACCC TGTGTGGGAA 
GAAACACTGA CATTTACAGT ACACATGCCA GAAATAGCTT TGGTTCGGTT CCTTGTGTGG 
GATCACGATC CCATTGGACG AGACTTTGTT GGACAAAGAA CTGTGACCTT CAGCAGCTTA 
GTGCCTGGCT ACCGGCATGT CTATTTGGAA GGACTGACAG AAGCATCCAT ATTTGTACAC 
ATAACCATCA ATGAAATCTA TGGAAAGTGG AGCCCTTTAA TACTCAACCC CAGTTATACT 
ATATTGCACT TTCTAGGAGC TACAAAGAAC AGACAACTCC AGGGTCTGAA GGGACTGTTC 
AATAAGAATC CTAGGCACAG TTCTTCAGAA AACAATTCCC ATTATGTACG GAAGCGATCC 
ATTGGAGATA GAATTCTGCG ACGCACAGCT AGCGCCCCAG CCAAAGGCAG GAAAAAGAGC 
AAAATGGGCT TCCAAGAAAT GGTGGAGATA AAGGATTCTG TGTCCGAGGC CACAAGAGAT 
CAAGATGGCG TGCTGAGGAG GACCACACGC AGTTTGCAAG CACGCCCTGT CTCTATGCCT 
GTTGACAGAA ACCTTCTGGG AGCTTTGTCG CTGCCTGTAT CTGAAACAGC AAAAGACATT 
GAAGGAAAAG AAAACTCTCT AGCAGAAGAT AAAGATGGCA GAAGAAAAGG GAAAGCAAGT 
ATAAAAGATC CACATTTTCT AAATTTCAAC AAAAAGTTAT CATCCTCCTC CAGTGCTCTG 
CTCCACAAAG ATACCAGCCA AGGGGACACC ATTGTATCTA CTGCCCACAT GTCAGTCACA 
GGAGAACAGC TGGGCATGTC AAGTCCTAGG GGTGGGAGAA CCACATCAAA TGCCACAAGC 
AATTGCCAGG AAAACCCCTG TCCCAGCAAG TCTCTCTCCC CAAAGCAGCA TTTGGCTCCC 
GATCCTGTAG TTAACCCCAC ACAAGATCTG CATGGTGTGA AAATCAAGGA AAAGGGTAAT 
CCTGAGGACT TTGTGGAAGG GAAAAGCATC TTGTCAGGAA GCGTCCTTTC TCATAGCAAC 
CTAGAAATTA AGAACCTGGA AGGTAATAGG GGTAAGGGCC GAGCTGCAAC ATCCTTTTCT 
TTGTCAGACG TCTCCATGCT CTGTTCTGAC ATACCTGACC TACATTCAAC TGCAATTCTG 
CAGGAGAGTG TAATTTCCCA TCTTATTGAC AATGTCACTT TAACAAATGA GAATGAGCCG 
GGCAGTTCCA TCTCAGCCCT GATTGGCCAG TTTGATGAGA CCAACAATCA GGCTCTCACA 
GTTGTTTCTC ATCTTCATAA TACCAGTGTG ATGTCAGGCC ATTGTCCCTT GCCTAGCCTG 
GGCCTAAAAA TGCCCATCAA GCATGGTTTT TGCAAGGGAA AATCCAAGTC TTCCTTCCTG 
TGCTCATCTC CGGAGCTGAT AGCACTCTCG AGTTCTGAGA CCACCAAACA TGCAACGAAC 
ACAGTTTATG AAACTACCTG CACTCCCATC TCTAAAACCA AACCAGATGA TGACCTTTCT 
AGTAAGGCCA AGACAGCGGC CTTAGAAAGC AACCTGCCTG GATCCCCTAA TACTTCTCGT 
GGCTGGTTAC CAAAAAGTCC TACCAAGGGA GAAGACTGGG AAACACTGAA GAGCTGCAGC 
CCTGCCTCTT CCCCTGATTT GACCCTGGAG GATGTAATAG CTGATCCCAC TCTCTGTTTC 
AATTCTGGGG AGAGCAGCCT TGTGGAAATT GATGGAGAAT CAGAAAATCT TTCTCTAACA 
ACCTGTGAAT ATAGGAGAGA GGGCACAAGT CAACTTGCTT CTCCTTTAAA ACTCAAGTAC 
AATCAGGGTG TGGTAGAACA CTTTCAAAGA GGTTTGAGAA ACGGCTACTG TAAAGAGACC 
CTCCGCCCTT CTGTCCCTGA AATATTCAAC AATATTCAAG ATGTCAAAAC TCAAAGTATT 
TCTTATCTAG CCTATCAGGG TGCTGGCTTT GTGCATAATC ATTTCTCAGA TTCAGATGCA 
AAAATGTTCC AGACCTGTGT GCCCCAGCAA TCTAGTGCTC AAGATATGCA TGTCCCTGTA 
CCCAAGCAGT TGGCACATCT TCCTTTGCCT GCTCTGAAAC TGCCTAGTCC TTGCAAATCC 
AAAAGTCTGG GGGACTTAAC ATCAGAGGAC ATTGCCTGCA ATTTTGAGAG CAAGTACCAG 
TGTATTAGTA AGAGTTTTGT TACAACTGGC ATTAGAGACA AGAAGGGCGT GACTGTGAAG 
ACAAAGTCGT TAGAGCCTAT AGATGCCCTG ACCGAGCAGC TTCGGAAGCT TGTGTCCTTT 
GACCAGGAAG ACAACTGCCA AGTGCTATAT TCAAAGCAGG ATGCCAATCA GCTCCCCCGG 
GCACTGGTCA GGAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AACAGGAAGC CAACAAGCAG AAAGTTCCAA ACCCCAGCAA TGGGGCAGGA 
GTGGTTCTTA GAAACAAACC CTCAGCACCC ACCCCTGCAG TGAATCGCCA CTCCACCGGC 
TCCTACATCG CAGGCTACCT GAAGAACACG AAAGGGGGTG GCCTTGAAGG CCGGGGCATC 
CCAGAGGGGG CATGCACGGC TCTTCACTAT GGCCACGTTG ACCAGTTTTG TTCAGATAAT 
TCTGTTTTGC AGACTGAGCC AAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861414.1
CDS	291..5336
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017005925.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005925.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAAAACTAC AAAATCACGG TCTAAATCTT ACAGTACTGA TGATGAGGAA 
GACACACAGC AGAGTACTGG CAAGGAGGGT GGCCAGCTGT ACAGATTGGG TCGCCGAAGG 
AAAACCATGA AGCTCTGCCG AGAACTCTCT GATTTGGTTG TGTACACAAA CTCCGTGGCC 
GCTCAGGACA TTGTGGATGA CGGAACCACA GGAAATGTGT TATCATTCAG TGAAACAAGA 
GCACATCAGG TTGTTCAGCA AAAATCAGAG CAGTTCATGA TTTATAATCA AAAGCAACTC 
ACGAGGATTT ACCCCTCTGC CTACCGCATT GATTCCAGTA ACTTCAACCC TCTCCCCTAC 
TGGAACGCAG GCTGCCAGCT AGTGGCACTG AATTATCAAT CTGAAGGACG AATGATGCAG 
TTAAACCGAG CCAAATTCAA GGCAAATGGC AATTGTGGCT ATGTCCTCAA ACCCCAGCAA 
ATGTGCAAAG GTACTTTCAA CCCTTTCTCT GGTGACCCTC TTCCTGCCAA CCCCAAAAAG 
CAGCTCATCC TGAAAGTTAT CAGTGGACAG CAACTCCCCA AACCTCCAGA CTCCATGTTT 
GGAGATCGAG GCGAGATCAT TGACCCTTTT GTTGAAGTTG AAATTATTGG ATTGCCAGTA 
GATTGTTGTA AAGATCAAAC CCGTGTGGTA GATGACAATG GATTTAACCC TGTGTGGGAA 
GAAACACTGA CATTTACAGT ACACATGCCA GAAATAGCTT TGGTTCGGTT CCTTGTGTGG 
GATCACGATC CCATTGGACG AGACTTTGTT GGACAAAGAA CTGTGACCTT CAGCAGCTTA 
GTGCCTGGCT ACCGGCATGT CTATTTGGAA GGACTGACAG AAGCATCCAT ATTTGTACAC 
ATAACCATCA ATGAAATCTA TGGAAAGTGG AGCCCTTTAA TACTCAACCC CAGTTATACT 
ATATTGCACT TTCTAGGAGC TACAAAGAAC AGACAACTCC AGGGTCTGAA GGGACTGTTC 
AATAAGAATC CTAGGCACAG TTCTTCAGAA AACAATTCCC ATTATGTACG GAAGCGATCC 
ATTGGAGATA GAATTCTGCG ACGCACAGCT AGCGCCCCAG CCAAAGGCAG GAAAAAGAGC 
AAAATGGGCT TCCAAGAAAT GGTGGAGATA AAGGATTCTG TGTCCGAGGC CACAAGAGAT 
CAAGATGGCG TGCTGAGGAG GACCACACGC AGTTTGCAAG CACGCCCTGT CTCTATGCCT 
GTTGACAGAA ACCTTCTGGG AGCTTTGTCG CTGCCTGTAT CTGAAACAGC AAAAGACATT 
GAAGGAAAAG AAAACTCTCT AGCAGAAGAT AAAGATGGCA GAAGAAAAGG GAAAGCAAGT 
ATAAAAGATC CACATTTTCT AAATTTCAAC AAAAAGTTAT CATCCTCCTC CAGTGCTCTG 
CTCCACAAAG ATACCAGCCA AGGGGACACC ATTGTATCTA CTGCCCACAT GTCAGTCACA 
GGAGAACAGC TGGGCATGTC AAGTCCTAGG GGTGGGAGAA CCACATCAAA TGCCACAAGC 
AATTGCCAGG AAAACCCCTG TCCCAGCAAG TCTCTCTCCC CAAAGCAGCA TTTGGCTCCC 
GATCCTGTAG TTAACCCCAC ACAAGATCTG CATGGTGTGA AAATCAAGGA AAAGGGTAAT 
CCTGAGGACT TTGTGGAAGG GAAAAGCATC TTGTCAGGAA GCGTCCTTTC TCATAGCAAC 
CTAGAAATTA AGAACCTGGA AGGTAATAGG GGTAAGGGCC GAGCTGCAAC ATCCTTTTCT 
TTGTCAGACG TCTCCATGCT CTGTTCTGAC ATACCTGACC TACATTCAAC TGCAATTCTG 
CAGGAGAGTG TAATTTCCCA TCTTATTGAC AATGTCACTT TAACAAATGA GAATGAGCCG 
GGCAGTTCCA TCTCAGCCCT GATTGGCCAG TTTGATGAGA CCAACAATCA GGCTCTCACA 
GTTGTTTCTC ATCTTCATAA TACCAGTGTG ATGTCAGGCC ATTGTCCCTT GCCTAGCCTG 
GGCCTAAAAA TGCCCATCAA GCATGGTTTT TGCAAGGGAA AATCCAAGTC TTCCTTCCTG 
TGCTCATCTC CGGAGCTGAT AGCACTCTCG AGTTCTGAGA CCACCAAACA TGCAACGAAC 
ACAGTTTATG AAACTACCTG CACTCCCATC TCTAAAACCA AACCAGATGA TGACCTTTCT 
AGTAAGGCCA AGACAGCGGC CTTAGAAAGC AACCTGCCTG GATCCCCTAA TACTTCTCGT 
GGCTGGTTAC CAAAAAGTCC TACCAAGGGA GAAGACTGGG AAACACTGAA GAGCTGCAGC 
CCTGCCTCTT CCCCTGATTT GACCCTGGAG GATGTAATAG CTGATCCCAC TCTCTGTTTC 
AATTCTGGGG AGAGCAGCCT TGTGGAAATT GATGGAGAAT CAGAAAATCT TTCTCTAACA 
ACCTGTGAAT ATAGGAGAGA GGGCACAAGT CAACTTGCTT CTCCTTTAAA ACTCAAGTAC 
AATCAGGGTG TGGTAGAACA CTTTCAAAGA GGTTTGAGAA ACGGCTACTG TAAAGAGACC 
CTCCGCCCTT CTGTCCCTGA AATATTCAAC AATATTCAAG ATGTCAAAAC TCAAAGTATT 
TCTTATCTAG CCTATCAGGG TGCTGGCTTT GTGCATAATC ATTTCTCAGA TTCAGATGCA 
AAAATGTTCC AGACCTGTGT GCCCCAGCAA TCTAGTGCTC AAGATATGCA TGTCCCTGTA 
CCCAAGCAGT TGGCACATCT TCCTTTGCCT GCTCTGAAAC TGCCTAGTCC TTGCAAATCC 
AAAAGTCTGG GGGACTTAAC ATCAGAGGAC ATTGCCTGCA ATTTTGAGAG CAAGTACCAG 
TGTATTAGTA AGAGTTTTGT TACAACTGGC ATTAGAGACA AGAAGGGCGT GACTGTGAAG 
ACAAAGTCGT TAGAGCCTAT AGATGCCCTG ACCGAGCAGC TTCGGAAGCT TGTGTCCTTT 
GACCAGGAAG ACAACTGCCA AGTGCTATAT TCAAAGCAGG ATGCCAATCA GCTCCCCCGG 
GCACTGGTCA GGAAGTTGTC ATCCAGAAGT CAGAGCAGAG TGCGCAATAT TGCTAGTCGT 
GCCAAGGAGA AACAGGAAGC CAACAAGCAG AAAGTTCCAA ACCCCAGCAA TGGGGCAGGA 
GTGGTTCTTA GAAACAAACC CTCAGCACCC ACCCCTGCAG TGAATCGCCA CTCCACCGGC 
TCCTACATCG CAGGCTACCT GAAGAACACG AAAGGGGGTG GCCTTGAAGG CCGGGGCATC 
CCAGAGGGGG CATGCACGGC TCTTCACTAT GGCCACGTTG ACCAGTTTTG TTCAGATAAT 
TCTGTTTTGC AGACTGAGCC AAGCAGTGAT GATAAACCAG AAATTTATTT TCTTTTGAGA 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001130960.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124432.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 1, mRNA.

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CloneID	OHu31414
Clone ID Related Accession (Same CDS sequence)	NM_001130960.2 , NM_001130960.1
Accession Version	NM_001130960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132006.2, DA206803.1, AK096620.1, BC113950.1, AY691171.1 and AC012022.13. On Jun 2, 2019 this sequence version replaced NM_001130960.1. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.24886.1, AY691171.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGCA 
GAAGATAAAG ATGGCAGAAG AAAAGGGAAA GCAAGTATAA AAGATCCACA TTTTCTAAAT 
TTCAACAAAA AGTTATCATC CTCCTCCAGT GCTCTGCTCC ACAAAGATAC CAGCCAAGGG 
GACACCATTG TATCTACTGC CCACATGTCA GTCACAGGAG AACAGCTGGG CATGTCAAGT 
CCTAGGGGTG GGAGAACCAC ATCAAATGCC ACAAGCAATT GCCAGGAAAA CCCCTGTCCC 
AGCAAGTCTC TCTCCCCAAA GCAGCATTTG GCTCCCGATC CTGTAGTTAA CCCCACACAA 
GATCTGCATG GTGTGAAAAT CAAGGAAAAG GGTAATCCTG AGGACTTTGT GGAAGGGAAA 
AGCATCTTGT CAGGAAGCGT CCTTTCTCAT AGCAACCTAG AAATTAAGAA CCTGGAAGGT 
AATAGGGGTA AGGGCCGAGC TGCAACATCC TTTTCTTTGT CAGACGTCTC CATGCTCTGT 
TCTGACATAC CTGACCTACA TTCAACTGCA ATTCTGCAGG AGAGTGTAAT TTCCCATCTT 
ATTGACAATG TCACTTTAAC AAATGAGAAT GAGCCGGGCA GTTCCATCTC AGCCCTGATT 
GGCCAGTTTG ATGAGACCAA CAATCAGGCT CTCACAGTTG TTTCTCATCT TCATAATACC 
AGTGTGATGT CAGGCCATTG TCCCTTGCCT AGCCTGGGCC TAAAAATGCC CATCAAGCAT 
GGTTTTTGCA AGGGAAAATC CAAGTCTTCC TTCCTGTGCT CATCTCCGGA GCTGATAGCA 
CTCTCGAGTT CTGAGACCAC CAAACATGCA ACGAACACAG TTTATGAAAC TACCTGCACT 
CCCATCTCTA AAACCAAACC AGATGATGAC CTTTCTAGTA AGGCCAAGAC AGCGGCCTTA 
GAAAGCAACC TGCCTGGATC CCCTAATACT TCTCGTGGCT GGTTACCAAA AAGTCCTACC 
AAGGGAGAAG ACTGGGAAAC ACTGAAGAGC TGCAGCCCTG CCTCTTCCCC TGATTTGACC 
CTGGAGGATG TAATAGCTGA TCCCACTCTC TGTTTCAATT CTGGGGAGAG CAGCCTTGTG 
GAAATTGATG GAGAATCAGA AAATCTTTCT CTAACAACCT GTGAATATAG GAGAGAGGGC 
ACAAGTCAAC TTGCTTCTCC TTTAAAACTC AAGTACAATC AGGGTGTGGT AGAACACTTT 
CAAAGAGGTT TGAGAAACGG CTACTGTAAA GAGACCCTCC GCCCTTCTGT CCCTGAAATA 
TTCAACAATA TTCAAGATGT CAAAACTCAA AGTATTTCTT ATCTAGCCTA TCAGGGTGCT 
GGCTTTGTGC ATAATCATTT CTCAGATTCA GATGCAAAAA TGTTCCAGAC CTGTGTGCCC 
CAGCAATCTA GTGCTCAAGA TATGCATGTC CCTGTACCCA AGCAGTTGGC ACATCTTCCT 
TTGCCTGCTC TGAAACTGCC TAGTCCTTGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAGAGCAAG TACCAGTGTA TTAGTAAGAG TTTTGTTACA 
ACTGGCATTA GAGACAAGAA GGGCGTGACT GTGAAGACAA AGTCGTTAGA GCCTATAGAT 
GCCCTGACCG AGCAGCTTCG GAAGCTTGTG TCCTTTGACC AGGAAGACAA CTGCCAAGTG 
CTATATTCAA AGCAGGATGC CAATCAGCTC CCCCGGGCAC TGGTCAGGAA GTTGTCATCC 
AGAAGTCAGA GCAGAGTGCG CAATATTGCT AGTCGTGCCA AGGAGAAACA GGAAGCCAAC 
AAGCAGAAAG TTCCAAACCC CAGCAATGGG GCAGGAGTGG TTCTTAGAAA CAAACCCTCA 
GCACCCACCC CTGCAGTGAA TCGCCACTCC ACCGGCTCCT ACATCGCAGG CTACCTGAAG 
AACACGAAAG GGGGTGGCCT TGAAGGCCGG GGCATCCCAG AGGGGGCATG CACGGCTCTT 
CACTATGGCC ACGTTGACCA GTTTTGTTCA GATAATTCTG TTTTGCAGAC TGAGCCAAGC 
AGTGATGATA AACCAGAAAT TTATTTTCTT TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124432.1
CDS	305..5386
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_001130960.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130960.2

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGCA 
GAAGATAAAG ATGGCAGAAG AAAAGGGAAA GCAAGTATAA AAGATCCACA TTTTCTAAAT 
TTCAACAAAA AGTTATCATC CTCCTCCAGT GCTCTGCTCC ACAAAGATAC CAGCCAAGGG 
GACACCATTG TATCTACTGC CCACATGTCA GTCACAGGAG AACAGCTGGG CATGTCAAGT 
CCTAGGGGTG GGAGAACCAC ATCAAATGCC ACAAGCAATT GCCAGGAAAA CCCCTGTCCC 
AGCAAGTCTC TCTCCCCAAA GCAGCATTTG GCTCCCGATC CTGTAGTTAA CCCCACACAA 
GATCTGCATG GTGTGAAAAT CAAGGAAAAG GGTAATCCTG AGGACTTTGT GGAAGGGAAA 
AGCATCTTGT CAGGAAGCGT CCTTTCTCAT AGCAACCTAG AAATTAAGAA CCTGGAAGGT 
AATAGGGGTA AGGGCCGAGC TGCAACATCC TTTTCTTTGT CAGACGTCTC CATGCTCTGT 
TCTGACATAC CTGACCTACA TTCAACTGCA ATTCTGCAGG AGAGTGTAAT TTCCCATCTT 
ATTGACAATG TCACTTTAAC AAATGAGAAT GAGCCGGGCA GTTCCATCTC AGCCCTGATT 
GGCCAGTTTG ATGAGACCAA CAATCAGGCT CTCACAGTTG TTTCTCATCT TCATAATACC 
AGTGTGATGT CAGGCCATTG TCCCTTGCCT AGCCTGGGCC TAAAAATGCC CATCAAGCAT 
GGTTTTTGCA AGGGAAAATC CAAGTCTTCC TTCCTGTGCT CATCTCCGGA GCTGATAGCA 
CTCTCGAGTT CTGAGACCAC CAAACATGCA ACGAACACAG TTTATGAAAC TACCTGCACT 
CCCATCTCTA AAACCAAACC AGATGATGAC CTTTCTAGTA AGGCCAAGAC AGCGGCCTTA 
GAAAGCAACC TGCCTGGATC CCCTAATACT TCTCGTGGCT GGTTACCAAA AAGTCCTACC 
AAGGGAGAAG ACTGGGAAAC ACTGAAGAGC TGCAGCCCTG CCTCTTCCCC TGATTTGACC 
CTGGAGGATG TAATAGCTGA TCCCACTCTC TGTTTCAATT CTGGGGAGAG CAGCCTTGTG 
GAAATTGATG GAGAATCAGA AAATCTTTCT CTAACAACCT GTGAATATAG GAGAGAGGGC 
ACAAGTCAAC TTGCTTCTCC TTTAAAACTC AAGTACAATC AGGGTGTGGT AGAACACTTT 
CAAAGAGGTT TGAGAAACGG CTACTGTAAA GAGACCCTCC GCCCTTCTGT CCCTGAAATA 
TTCAACAATA TTCAAGATGT CAAAACTCAA AGTATTTCTT ATCTAGCCTA TCAGGGTGCT 
GGCTTTGTGC ATAATCATTT CTCAGATTCA GATGCAAAAA TGTTCCAGAC CTGTGTGCCC 
CAGCAATCTA GTGCTCAAGA TATGCATGTC CCTGTACCCA AGCAGTTGGC ACATCTTCCT 
TTGCCTGCTC TGAAACTGCC TAGTCCTTGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAGAGCAAG TACCAGTGTA TTAGTAAGAG TTTTGTTACA 
ACTGGCATTA GAGACAAGAA GGGCGTGACT GTGAAGACAA AGTCGTTAGA GCCTATAGAT 
GCCCTGACCG AGCAGCTTCG GAAGCTTGTG TCCTTTGACC AGGAAGACAA CTGCCAAGTG 
CTATATTCAA AGCAGGATGC CAATCAGCTC CCCCGGGCAC TGGTCAGGAA GTTGTCATCC 
AGAAGTCAGA GCAGAGTGCG CAATATTGCT AGTCGTGCCA AGGAGAAACA GGAAGCCAAC 
AAGCAGAAAG TTCCAAACCC CAGCAATGGG GCAGGAGTGG TTCTTAGAAA CAAACCCTCA 
GCACCCACCC CTGCAGTGAA TCGCCACTCC ACCGGCTCCT ACATCGCAGG CTACCTGAAG 
AACACGAAAG GGGGTGGCCT TGAAGGCCGG GGCATCCCAG AGGGGGCATG CACGGCTCTT 
CACTATGGCC ACGTTGACCA GTTTTGTTCA GATAATTCTG TTTTGCAGAC TGAGCCAAGC 
AGTGATGATA AACCAGAAAT TTATTTTCTT TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_001130960.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124432.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 1, mRNA.

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CloneID	OHu31414
Clone ID Related Accession (Same CDS sequence)	NM_001130960.2 , NM_001130960.1
Accession Version	NM_001130960.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA206803.1, AK096620.1, BC113950.1 and AY691171.1. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: AY691171.1, SRR1803615.24886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGCA 
GAAGATAAAG ATGGCAGAAG AAAAGGGAAA GCAAGTATAA AAGATCCACA TTTTCTAAAT 
TTCAACAAAA AGTTATCATC CTCCTCCAGT GCTCTGCTCC ACAAAGATAC CAGCCAAGGG 
GACACCATTG TATCTACTGC CCACATGTCA GTCACAGGAG AACAGCTGGG CATGTCAAGT 
CCTAGGGGTG GGAGAACCAC ATCAAATGCC ACAAGCAATT GCCAGGAAAA CCCCTGTCCC 
AGCAAGTCTC TCTCCCCAAA GCAGCATTTG GCTCCCGATC CTGTAGTTAA CCCCACACAA 
GATCTGCATG GTGTGAAAAT CAAGGAAAAG GGTAATCCTG AGGACTTTGT GGAAGGGAAA 
AGCATCTTGT CAGGAAGCGT CCTTTCTCAT AGCAACCTAG AAATTAAGAA CCTGGAAGGT 
AATAGGGGTA AGGGCCGAGC TGCAACATCC TTTTCTTTGT CAGACGTCTC CATGCTCTGT 
TCTGACATAC CTGACCTACA TTCAACTGCA ATTCTGCAGG AGAGTGTAAT TTCCCATCTT 
ATTGACAATG TCACTTTAAC AAATGAGAAT GAGCCGGGCA GTTCCATCTC AGCCCTGATT 
GGCCAGTTTG ATGAGACCAA CAATCAGGCT CTCACAGTTG TTTCTCATCT TCATAATACC 
AGTGTGATGT CAGGCCATTG TCCCTTGCCT AGCCTGGGCC TAAAAATGCC CATCAAGCAT 
GGTTTTTGCA AGGGAAAATC CAAGTCTTCC TTCCTGTGCT CATCTCCGGA GCTGATAGCA 
CTCTCGAGTT CTGAGACCAC CAAACATGCA ACGAACACAG TTTATGAAAC TACCTGCACT 
CCCATCTCTA AAACCAAACC AGATGATGAC CTTTCTAGTA AGGCCAAGAC AGCGGCCTTA 
GAAAGCAACC TGCCTGGATC CCCTAATACT TCTCGTGGCT GGTTACCAAA AAGTCCTACC 
AAGGGAGAAG ACTGGGAAAC ACTGAAGAGC TGCAGCCCTG CCTCTTCCCC TGATTTGACC 
CTGGAGGATG TAATAGCTGA TCCCACTCTC TGTTTCAATT CTGGGGAGAG CAGCCTTGTG 
GAAATTGATG GAGAATCAGA AAATCTTTCT CTAACAACCT GTGAATATAG GAGAGAGGGC 
ACAAGTCAAC TTGCTTCTCC TTTAAAACTC AAGTACAATC AGGGTGTGGT AGAACACTTT 
CAAAGAGGTT TGAGAAACGG CTACTGTAAA GAGACCCTCC GCCCTTCTGT CCCTGAAATA 
TTCAACAATA TTCAAGATGT CAAAACTCAA AGTATTTCTT ATCTAGCCTA TCAGGGTGCT 
GGCTTTGTGC ATAATCATTT CTCAGATTCA GATGCAAAAA TGTTCCAGAC CTGTGTGCCC 
CAGCAATCTA GTGCTCAAGA TATGCATGTC CCTGTACCCA AGCAGTTGGC ACATCTTCCT 
TTGCCTGCTC TGAAACTGCC TAGTCCTTGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAGAGCAAG TACCAGTGTA TTAGTAAGAG TTTTGTTACA 
ACTGGCATTA GAGACAAGAA GGGCGTGACT GTGAAGACAA AGTCGTTAGA GCCTATAGAT 
GCCCTGACCG AGCAGCTTCG GAAGCTTGTG TCCTTTGACC AGGAAGACAA CTGCCAAGTG 
CTATATTCAA AGCAGGATGC CAATCAGCTC CCCCGGGCAC TGGTCAGGAA GTTGTCATCC 
AGAAGTCAGA GCAGAGTGCG CAATATTGCT AGTCGTGCCA AGGAGAAACA GGAAGCCAAC 
AAGCAGAAAG TTCCAAACCC CAGCAATGGG GCAGGAGTGG TTCTTAGAAA CAAACCCTCA 
GCACCCACCC CTGCAGTGAA TCGCCACTCC ACCGGCTCCT ACATCGCAGG CTACCTGAAG 
AACACGAAAG GGGGTGGCCT TGAAGGCCGG GGCATCCCAG AGGGGGCATG CACGGCTCTT 
CACTATGGCC ACGTTGACCA GTTTTGTTCA GATAATTCTG TTTTGCAGAC TGAGCCAAGC 
AGTGATGATA AACCAGAAAT TTATTTTCTT TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124432.1
CDS	278..5359
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_001130960.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130960.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGTGGAGCC CTTTAATACT CAACCCCAGT TATACTATAT TGCACTTTCT AGGAGCTACA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGCA 
GAAGATAAAG ATGGCAGAAG AAAAGGGAAA GCAAGTATAA AAGATCCACA TTTTCTAAAT 
TTCAACAAAA AGTTATCATC CTCCTCCAGT GCTCTGCTCC ACAAAGATAC CAGCCAAGGG 
GACACCATTG TATCTACTGC CCACATGTCA GTCACAGGAG AACAGCTGGG CATGTCAAGT 
CCTAGGGGTG GGAGAACCAC ATCAAATGCC ACAAGCAATT GCCAGGAAAA CCCCTGTCCC 
AGCAAGTCTC TCTCCCCAAA GCAGCATTTG GCTCCCGATC CTGTAGTTAA CCCCACACAA 
GATCTGCATG GTGTGAAAAT CAAGGAAAAG GGTAATCCTG AGGACTTTGT GGAAGGGAAA 
AGCATCTTGT CAGGAAGCGT CCTTTCTCAT AGCAACCTAG AAATTAAGAA CCTGGAAGGT 
AATAGGGGTA AGGGCCGAGC TGCAACATCC TTTTCTTTGT CAGACGTCTC CATGCTCTGT 
TCTGACATAC CTGACCTACA TTCAACTGCA ATTCTGCAGG AGAGTGTAAT TTCCCATCTT 
ATTGACAATG TCACTTTAAC AAATGAGAAT GAGCCGGGCA GTTCCATCTC AGCCCTGATT 
GGCCAGTTTG ATGAGACCAA CAATCAGGCT CTCACAGTTG TTTCTCATCT TCATAATACC 
AGTGTGATGT CAGGCCATTG TCCCTTGCCT AGCCTGGGCC TAAAAATGCC CATCAAGCAT 
GGTTTTTGCA AGGGAAAATC CAAGTCTTCC TTCCTGTGCT CATCTCCGGA GCTGATAGCA 
CTCTCGAGTT CTGAGACCAC CAAACATGCA ACGAACACAG TTTATGAAAC TACCTGCACT 
CCCATCTCTA AAACCAAACC AGATGATGAC CTTTCTAGTA AGGCCAAGAC AGCGGCCTTA 
GAAAGCAACC TGCCTGGATC CCCTAATACT TCTCGTGGCT GGTTACCAAA AAGTCCTACC 
AAGGGAGAAG ACTGGGAAAC ACTGAAGAGC TGCAGCCCTG CCTCTTCCCC TGATTTGACC 
CTGGAGGATG TAATAGCTGA TCCCACTCTC TGTTTCAATT CTGGGGAGAG CAGCCTTGTG 
GAAATTGATG GAGAATCAGA AAATCTTTCT CTAACAACCT GTGAATATAG GAGAGAGGGC 
ACAAGTCAAC TTGCTTCTCC TTTAAAACTC AAGTACAATC AGGGTGTGGT AGAACACTTT 
CAAAGAGGTT TGAGAAACGG CTACTGTAAA GAGACCCTCC GCCCTTCTGT CCCTGAAATA 
TTCAACAATA TTCAAGATGT CAAAACTCAA AGTATTTCTT ATCTAGCCTA TCAGGGTGCT 
GGCTTTGTGC ATAATCATTT CTCAGATTCA GATGCAAAAA TGTTCCAGAC CTGTGTGCCC 
CAGCAATCTA GTGCTCAAGA TATGCATGTC CCTGTACCCA AGCAGTTGGC ACATCTTCCT 
TTGCCTGCTC TGAAACTGCC TAGTCCTTGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAGAGCAAG TACCAGTGTA TTAGTAAGAG TTTTGTTACA 
ACTGGCATTA GAGACAAGAA GGGCGTGACT GTGAAGACAA AGTCGTTAGA GCCTATAGAT 
GCCCTGACCG AGCAGCTTCG GAAGCTTGTG TCCTTTGACC AGGAAGACAA CTGCCAAGTG 
CTATATTCAA AGCAGGATGC CAATCAGCTC CCCCGGGCAC TGGTCAGGAA GTTGTCATCC 
AGAAGTCAGA GCAGAGTGCG CAATATTGCT AGTCGTGCCA AGGAGAAACA GGAAGCCAAC 
AAGCAGAAAG TTCCAAACCC CAGCAATGGG GCAGGAGTGG TTCTTAGAAA CAAACCCTCA 
GCACCCACCC CTGCAGTGAA TCGCCACTCC ACCGGCTCCT ACATCGCAGG CTACCTGAAG 
AACACGAAAG GGGGTGGCCT TGAAGGCCGG GGCATCCCAG AGGGGGCATG CACGGCTCTT 
CACTATGGCC ACGTTGACCA GTTTTGTTCA GATAATTCTG TTTTGCAGAC TGAGCCAAGC 
AGTGATGATA AACCAGAAAT TTATTTTCTT TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( NM_014996.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055811.2 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2, mRNA.

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CloneID	OHu37790
Clone ID Related Accession (Same CDS sequence)	NM_014996.3 , XM_011512564.2 , NM_014996.4
Accession Version	NM_014996.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132006.2, BC113950.1, AC012022.13 and AB028992.1. On Mar 9, 2017 this sequence version replaced NM_014996.2. Transcript Variant: This variant (2) encodes isoform b. ##Evidence-Data-START## Transcript exon combination :: BC113950.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055811.2
CDS	41..5098
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	NM_014996.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014996.3

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512560.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510862.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu37789
Clone ID Related Accession (Same CDS sequence)	XM_011512560.3 , XM_011512561.2 , XM_005247238.1 , XM_011512562.3 , XM_011512562.2 , XM_011512560.2
Accession Version	XM_011512560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512560.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510862.1
CDS	301..5418
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512560.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512560.3

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512561.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510863.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

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CloneID	OHu37789
Clone ID Related Accession (Same CDS sequence)	XM_011512560.3 , XM_011512561.2 , XM_005247238.1 , XM_011512562.3 , XM_011512562.2 , XM_011512560.2
Accession Version	XM_011512561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510863.1
CDS	432..5549
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512561.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512561.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005247238.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247295.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

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CloneID	OHu37789
Clone ID Related Accession (Same CDS sequence)	XM_011512560.3 , XM_011512561.2 , XM_005247238.1 , XM_011512562.3 , XM_011512562.2 , XM_011512560.2
Accession Version	XM_005247238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247295.1
CDS	291..5408
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005247238.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247238.1

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512562.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510864.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OHu37789
Clone ID Related Accession (Same CDS sequence)	XM_011512560.3 , XM_011512561.2 , XM_005247238.1 , XM_011512562.3 , XM_011512562.2 , XM_011512560.2
Accession Version	XM_011512562.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510864.1
CDS	1050..6167
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512562.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512562.3

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512562.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510864.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

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CloneID	OHu37789
Clone ID Related Accession (Same CDS sequence)	XM_011512560.3 , XM_011512561.2 , XM_005247238.1 , XM_011512562.3 , XM_011512562.2 , XM_011512560.2
Accession Version	XM_011512562.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510864.1
CDS	1049..6166
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512562.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512562.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512560.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510862.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

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CloneID	OHu37789
Clone ID Related Accession (Same CDS sequence)	XM_011512560.3 , XM_011512561.2 , XM_005247238.1 , XM_011512562.3 , XM_011512562.2 , XM_011512560.2
Accession Version	XM_011512560.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510862.1
CDS	300..5417
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512560.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGT GGAGCCCTTT AATACTCAAC 
CCCAGTTATA CTATATTGCA CTTTCTAGGA GCTACAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512564.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510866.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

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CloneID	OHu37790
Clone ID Related Accession (Same CDS sequence)	NM_014996.3 , XM_011512564.2 , NM_014996.4
Accession Version	XM_011512564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510866.1
CDS	291..5348
Translation	MSYWNLEKRNCVQYRRHFLVDNSVFHVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512564.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512564.2

ORF Insert Sequence:

ATGAGTTACT GGAACTTGGA AAAAAGGAAC TGTGTGCAGT ACCGCAGGCA TTTTCTGGTG 
GACAACAGTG TGTTTCATGT TGAAAGATGC ATGAGTGTGA TGCAGTCCGG GACACAGATG 
ATCAAGCTGA AACGTGGAAC CAAAGGGCTT GTCCGCCTCT TTTACCTGGA TGAGCACCGG 
ACACGCCTCC GATGGCGACC CTCTAGGAAG AGTGAGAAGG CAAAAATACT TATTGATTCC 
ATTTACAAAG TGACTGAGGG CCGGCAGTCT GAAATATTCC ACAGACAAGC TGAGGGGAAC 
TTCGACCCCA GCTGCTGCTT CACCATCTAC CATGGCAACC ACATGGAGTC CCTGGACCTC 
ATCACCTCCA ACCCCGAGGA GGCCCGCACC TGGATCACAG GCCTCAAGTA CCTGATGGCT 
GGCATCAGTG ATGAAGACTC CCTTGCCAAA AGGCAGAGGA CCCATGACCA ATGGGTGAAG 
CAGACCTTTG AGGAAGCTGA TAAGAATGGT GACGGCTTGC TGAATATTGA AGAGATACAT 
CAGCTGATGC ATAAACTGAA TGTTAATCTG CCCCGAAGAA AAGTCAGACA AATGTTTCAG 
GAAGCCGACA CAGATGAGAA TCAGGGAACT TTGACATTTG AAGAGTTCTG TGTTTTTTAC 
AAAATGATGT CTTTGAGACG AGACCTTTAT TTGTTACTTT TGAGCTACAG TGACAAGAAA 
GATCACCTAA CTGTGGAAGA ACTGGCTCAG TTTTTGAAGG TGGAGCAAAA GATGAATAAT 
GTGACAACGG ACTATTGTCT TGACATCATA AAGAAGTTTG AAGTTTCAGA AGAAAATAAG 
GTGAAAAATG TTCTTGGCAT AGAAGGCTTC ACGAACTTCA TGCGTAGTCC TGCCTGTGAC 
ATATTTAACC CATTGCACCA TGAAGTGTAC CAAGACATGG ATCAGCCCCT CTGCAACTAC 
TACATTGCTT CCTCTCACAA TACATACCTG ACTGGAGACC AGCTCCTTTC TCAGTCCAAA 
GTGGATATGT ATGCACGGGT GCTGCAAGAG GGCTGTCGCT GTGTGGAAGT TGACTGTTGG 
GATGGCCCAG ATGGAGAGCC AGTAGTACAT CATGGTTACA CTCTCACTTC AAAAATTCTC 
TTCAGAGATG TTGTGGAGAC CATCAACAAG CATGCCTTTG TGAAGAATGA GTTTCCTGTT 
ATATTGTCTA TCGAGAATCA CTGCAGTATC CAGCAGCAAA GGAAGATTGC TCAGTACCTG 
AAAGGAATAT TCGGAGACAA ACTGGACCTG TCATCTGTTG ATACAGGGGA GTGCAAGCAG 
CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT 
CACCTTGGGG ATGATGCAGA GGAAGGGGAA GTTTCTGATG AGGACAGTGC AGATGAAATT 
GAAGACGAGT GCAAATTCAA GCTCCATTAT AGTAATGGGA CCACTGAGCA TCAGGTGGAA 
TCTTTCATAA GGAAAAAACT GGAGTCACTG TTAAAAGAAT CTCAAATTCG AGATAAAGAA 
GATCCTGATA GTTTCACAGT GCGGGCACTA CTGAAGGCCA CGCATGAAGG CTTAAATGCA 
CACCTGAAGC AGAGTCCAGA TGTAAAGGAA AGTGGAAAGA AATCACATGG ACGATCCCTC 
ATGACCAACT TTGGAAAACA TAAGAAAACT ACAAAATCAC GGTCTAAATC TTACAGTACT 
GATGATGAGG AAGACACACA GCAGAGTACT GGCAAGGAGG GTGGCCAGCT GTACAGATTG 
GGTCGCCGAA GGAAAACCAT GAAGCTCTGC CGAGAACTCT CTGATTTGGT TGTGTACACA 
AACTCCGTGG CCGCTCAGGA CATTGTGGAT GACGGAACCA CAGGAAATGT GTTATCATTC 
AGTGAAACAA GAGCACATCA GGTTGTTCAG CAAAAATCAG AGCAGTTCAT GATTTATAAT 
CAAAAGCAAC TCACGAGGAT TTACCCCTCT GCCTACCGCA TTGATTCCAG TAACTTCAAC 
CCTCTCCCCT ACTGGAACGC AGGCTGCCAG CTAGTGGCAC TGAATTATCA ATCTGAAGGA 
CGAATGATGC AGTTAAACCG AGCCAAATTC AAGGCAAATG GCAATTGTGG CTATGTCCTC 
AAACCCCAGC AAATGTGCAA AGGTACTTTC AACCCTTTCT CTGGTGACCC TCTTCCTGCC 
AACCCCAAAA AGCAGCTCAT CCTGAAAGTT ATCAGTGGAC AGCAACTCCC CAAACCTCCA 
GACTCCATGT TTGGAGATCG AGGCGAGATC ATTGACCCTT TTGTTGAAGT TGAAATTATT 
GGATTGCCAG TAGATTGTTG TAAAGATCAA ACCCGTGTGG TAGATGACAA TGGATTTAAC 
CCTGTGTGGG AAGAAACACT GACATTTACA GTACACATGC CAGAAATAGC TTTGGTTCGG 
TTCCTTGTGT GGGATCACGA TCCCATTGGA CGAGACTTTG TTGGACAAAG AACTGTGACC 
TTCAGCAGCT TAGTGCCTGG CTACCGGCAT GTCTATTTGG AAGGACTGAC AGAAGCATCC 
ATATTTGTAC ACATAACCAT CAATGAAATC TATGGAAAGA ACAGACAACT CCAGGGTCTG 
AAGGGACTGT TCAATAAGAA TCCTAGGCAC AGTTCTTCAG AAAACAATTC CCATTATGTA 
CGGAAGCGAT CCATTGGAGA TAGAATTCTG CGACGCACAG CTAGCGCCCC AGCCAAAGGC 
AGGAAAAAGA GCAAAATGGG CTTCCAAGAA ATGGTGGAGA TAAAGGATTC TGTGTCCGAG 
GCCACAAGAG ATCAAGATGG CGTGCTGAGG AGGACCACAC GCAGTTTGCA AGCACGCCCT 
GTCTCTATGC CTGTTGACAG AAACCTTCTG GGAGCTTTGT CGCTGCCTGT ATCTGAAACA 
GCAAAAGACA TTGAAGGAAA AGAAAACTCT CTAGCAGAAG ATAAAGATGG CAGAAGAAAA 
GGGAAAGCAA GTATAAAAGA TCCACATTTT CTAAATTTCA ACAAAAAGTT ATCATCCTCC 
TCCAGTGCTC TGCTCCACAA AGATACCAGC CAAGGGGACA CCATTGTATC TACTGCCCAC 
ATGTCAGTCA CAGGAGAACA GCTGGGCATG TCAAGTCCTA GGGGTGGGAG AACCACATCA 
AATGCCACAA GCAATTGCCA GGAAAACCCC TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG 
CATTTGGCTC CCGATCCTGT AGTTAACCCC ACACAAGATC TGCATGGTGT GAAAATCAAG 
GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT 
TCTCATAGCA ACCTAGAAAT TAAGAACCTG GAAGGTAATA GGGGTAAGGG CCGAGCTGCA 
ACATCCTTTT CTTTGTCAGA CGTCTCCATG CTCTGTTCTG ACATACCTGA CCTACATTCA 
ACTGCAATTC TGCAGGAGAG TGTAATTTCC CATCTTATTG ACAATGTCAC TTTAACAAAT 
GAGAATGAGC CGGGCAGTTC CATCTCAGCC CTGATTGGCC AGTTTGATGA GACCAACAAT 
CAGGCTCTCA CAGTTGTTTC TCATCTTCAT AATACCAGTG TGATGTCAGG CCATTGTCCC 
TTGCCTAGCC TGGGCCTAAA AATGCCCATC AAGCATGGTT TTTGCAAGGG AAAATCCAAG 
TCTTCCTTCC TGTGCTCATC TCCGGAGCTG ATAGCACTCT CGAGTTCTGA GACCACCAAA 
CATGCAACGA ACACAGTTTA TGAAACTACC TGCACTCCCA TCTCTAAAAC CAAACCAGAT 
GATGACCTTT CTAGTAAGGC CAAGACAGCG GCCTTAGAAA GCAACCTGCC TGGATCCCCT 
AATACTTCTC GTGGCTGGTT ACCAAAAAGT CCTACCAAGG GAGAAGACTG GGAAACACTG 
AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT TTGACCCTGG AGGATGTAAT AGCTGATCCC 
ACTCTCTGTT TCAATTCTGG GGAGAGCAGC CTTGTGGAAA TTGATGGAGA ATCAGAAAAT 
CTTTCTCTAA CAACCTGTGA ATATAGGAGA GAGGGCACAA GTCAACTTGC TTCTCCTTTA 
AAACTCAAGT ACAATCAGGG TGTGGTAGAA CACTTTCAAA GAGGTTTGAG AAACGGCTAC 
TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT GAAATATTCA ACAATATTCA AGATGTCAAA 
ACTCAAAGTA TTTCTTATCT AGCCTATCAG GGTGCTGGCT TTGTGCATAA TCATTTCTCA 
GATTCAGATG CAAAAATGTT CCAGACCTGT GTGCCCCAGC AATCTAGTGC TCAAGATATG 
CATGTCCCTG TACCCAAGCA GTTGGCACAT CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT 
CCTTGCAAAT CCAAAAGTCT GGGGGACTTA ACATCAGAGG ACATTGCCTG CAATTTTGAG 
AGCAAGTACC AGTGTATTAG TAAGAGTTTT GTTACAACTG GCATTAGAGA CAAGAAGGGC 
GTGACTGTGA AGACAAAGTC GTTAGAGCCT ATAGATGCCC TGACCGAGCA GCTTCGGAAG 
CTTGTGTCCT TTGACCAGGA AGACAACTGC CAAGTGCTAT ATTCAAAGCA GGATGCCAAT 
CAGCTCCCCC GGGCACTGGT CAGGAAGTTG TCATCCAGAA GTCAGAGCAG AGTGCGCAAT 
ATTGCTAGTC GTGCCAAGGA GAAACAGGAA GCCAACAAGC AGAAAGTTCC AAACCCCAGC 
AATGGGGCAG GAGTGGTTCT TAGAAACAAA CCCTCAGCAC CCACCCCTGC AGTGAATCGC 
CACTCCACCG GCTCCTACAT CGCAGGCTAC CTGAAGAACA CGAAAGGGGG TGGCCTTGAA 
GGCCGGGGCA TCCCAGAGGG GGCATGCACG GCTCTTCACT ATGGCCACGT TGACCAGTTT 
TGTTCAGATA ATTCTGTTTT GCAGACTGAG CCAAGCAGTG ATGATAAACC AGAAATTTAT 
TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_005247239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247296.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu37791
Clone ID Related Accession (Same CDS sequence)	XM_005247239.1
Accession Version	XM_005247239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGCA 
GAAGATAAAG ATGGCAGAAG AAAAGGGAAA GCAAGTATAA AAGATCCACA TTTTCTAAAT 
TTCAACAAAA AGTTATCATC CTCCTCCAGT GCTCTGCTCC ACAAAGATAC CAGCCAAGGG 
GACACCATTG TATCTACTGC CCACATGTCA GTCACAGGAG AACAGCTGGG CATGTCAAGT 
CCTAGGGGTG GGAGAACCAC ATCAAATGCC ACAAGCAATT GCCAGGAAAA CCCCTGTCCC 
AGCAAGTCTC TCTCCCCAAA GCAGCATTTG GCTCCCGATC CTGTAGTTAA CCCCACACAA 
GATCTGCATG GTGTGAAAAT CAAGGAAAAG GGTAATCCTG AGGACTTTGT GGAAGGGAAA 
AGCATCTTGT CAGGAAGCGT CCTTTCTCAT AGCAACCTAG AAATTAAGAA CCTGGAAGGT 
AATAGGGGTA AGGGCCGAGC TGCAACATCC TTTTCTTTGT CAGACGTCTC CATGCTCTGT 
TCTGACATAC CTGACCTACA TTCAACTGCA ATTCTGCAGG AGAGTGTAAT TTCCCATCTT 
ATTGACAATG TCACTTTAAC AAATGAGAAT GAGCCGGGCA GTTCCATCTC AGCCCTGATT 
GGCCAGTTTG ATGAGACCAA CAATCAGGCT CTCACAGTTG TTTCTCATCT TCATAATACC 
AGTGTGATGT CAGGCCATTG TCCCTTGCCT AGCCTGGGCC TAAAAATGCC CATCAAGCAT 
GGTTTTTGCA AGGGAAAATC CAAGTCTTCC TTCCTGTGCT CATCTCCGGA GCTGATAGCA 
CTCTCGAGTT CTGAGACCAC CAAACATGCA ACGAACACAG TTTATGAAAC TACCTGCACT 
CCCATCTCTA AAACCAAACC AGATGATGAC CTTTCTAGTA AGGCCAAGAC AGCGGCCTTA 
GAAAGCAACC TGCCTGGATC CCCTAATACT TCTCGTGGCT GGTTACCAAA AAGTCCTACC 
AAGGGAGAAG ACTGGGAAAC ACTGAAGAGC TGCAGCCCTG CCTCTTCCCC TGATTTGACC 
CTGGAGGATG TAATAGCTGA TCCCACTCTC TGTTTCAATT CTGGGGAGAG CAGCCTTGTG 
GAAATTGATG GAGAATCAGA AAATCTTTCT CTAACAACCT GTGAATATAG GAGAGAGGGC 
ACAAGTCAAC TTGCTTCTCC TTTAAAACTC AAGTACAATC AGGGTGTGGT AGAACACTTT 
CAAAGAGGTT TGAGAAACGG CTACTGTAAA GAGACCCTCC GCCCTTCTGT CCCTGAAATA 
TTCAACAATA TTCAAGATGT CAAAACTCAA AGTATTTCTT ATCTAGCCTA TCAGGGTGCT 
GGCTTTGTGC ATAATCATTT CTCAGATTCA GATGCAAAAA TGTTCCAGAC CTGTGTGCCC 
CAGCAATCTA GTGCTCAAGA TATGCATGTC CCTGTACCCA AGCAGTTGGC ACATCTTCCT 
TTGCCTGCTC TGAAACTGCC TAGTCCTTGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAGAGCAAG TACCAGTGTA TTAGTAAGAG TTTTGTTACA 
ACTGGCATTA GAGACAAGAA GGGCGTGACT GTGAAGACAA AGTCGTTAGA GCCTATAGAT 
GCCCTGACCG AGCAGCTTCG GAAGCTTGTG TCCTTTGACC AGGAAGACAA CTGCCAAGTG 
CTATATTCAA AGCAGGATGC CAATCAGCTC CCCCGGGCAC TGGTCAGGAA GTTGTCATCC 
AGAAGTCAGA GCAGAGTGCG CAATATTGCT AGTCGTGCCA AGGAGAAACA GGAAGCCAAC 
AAGCAGAAAG TTCCAAACCC CAGCAATGGG GCAGGAGTGG TTCTTAGAAA CAAACCCTCA 
GCACCCACCC CTGCAGTGAA TCGCCACTCC ACCGGCTCCT ACATCGCAGG CTACCTGAAG 
AACACGAAAG GGGGTGGCCT TGAAGGCCGG GGCATCCCAG AGGGGGCATG CACGGCTCTT 
CACTATGGCC ACGTTGACCA GTTTTGTTCA GATAATTCTG TTTTGCAGAC TGAGCCAAGC 
AGTGATGATA AACCAGAAAT TTATTTTCTT TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247296.1
CDS	378..5399
Translation	MADLEVYKNLSPEKVERCMSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_005247239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247239.1

ORF Insert Sequence:

ATGGCAGACC TTGAAGTGTA TAAAAACTTA AGTCCAGAAA AAGTTGAAAG ATGCATGAGT 
GTGATGCAGT CCGGGACACA GATGATCAAG CTGAAACGTG GAACCAAAGG GCTTGTCCGC 
CTCTTTTACC TGGATGAGCA CCGGACACGC CTCCGATGGC GACCCTCTAG GAAGAGTGAG 
AAGGCAAAAA TACTTATTGA TTCCATTTAC AAAGTGACTG AGGGCCGGCA GTCTGAAATA 
TTCCACAGAC AAGCTGAGGG GAACTTCGAC CCCAGCTGCT GCTTCACCAT CTACCATGGC 
AACCACATGG AGTCCCTGGA CCTCATCACC TCCAACCCCG AGGAGGCCCG CACCTGGATC 
ACAGGCCTCA AGTACCTGAT GGCTGGCATC AGTGATGAAG ACTCCCTTGC CAAAAGGCAG 
AGGACCCATG ACCAATGGGT GAAGCAGACC TTTGAGGAAG CTGATAAGAA TGGTGACGGC 
TTGCTGAATA TTGAAGAGAT ACATCAGCTG ATGCATAAAC TGAATGTTAA TCTGCCCCGA 
AGAAAAGTCA GACAAATGTT TCAGGAAGCC GACACAGATG AGAATCAGGG AACTTTGACA 
TTTGAAGAGT TCTGTGTTTT TTACAAAATG ATGTCTTTGA GACGAGACCT TTATTTGTTA 
CTTTTGAGCT ACAGTGACAA GAAAGATCAC CTAACTGTGG AAGAACTGGC TCAGTTTTTG 
AAGGTGGAGC AAAAGATGAA TAATGTGACA ACGGACTATT GTCTTGACAT CATAAAGAAG 
TTTGAAGTTT CAGAAGAAAA TAAGGTGAAA AATGTTCTTG GCATAGAAGG CTTCACGAAC 
TTCATGCGTA GTCCTGCCTG TGACATATTT AACCCATTGC ACCATGAAGT GTACCAAGAC 
ATGGATCAGC CCCTCTGCAA CTACTACATT GCTTCCTCTC ACAATACATA CCTGACTGGA 
GACCAGCTCC TTTCTCAGTC CAAAGTGGAT ATGTATGCAC GGGTGCTGCA AGAGGGCTGT 
CGCTGTGTGG AAGTTGACTG TTGGGATGGC CCAGATGGAG AGCCAGTAGT ACATCATGGT 
TACACTCTCA CTTCAAAAAT TCTCTTCAGA GATGTTGTGG AGACCATCAA CAAGCATGCC 
TTTGTGAAGA ATGAGTTTCC TGTTATATTG TCTATCGAGA ATCACTGCAG TATCCAGCAG 
CAAAGGAAGA TTGCTCAGTA CCTGAAAGGA ATATTCGGAG ACAAACTGGA CCTGTCATCT 
GTTGATACAG GGGAGTGCAA GCAGCTTCCA AGCCCTCAAA GTTTGAAAGG CAAAATTCTA 
GTGAAGGGTA AGAAGTTGCC TTATCACCTT GGGGATGATG CAGAGGAAGG GGAAGTTTCT 
GATGAGGACA GTGCAGATGA AATTGAAGAC GAGTGCAAAT TCAAGCTCCA TTATAGTAAT 
GGGACCACTG AGCATCAGGT GGAATCTTTC ATAAGGAAAA AACTGGAGTC ACTGTTAAAA 
GAATCTCAAA TTCGAGATAA AGAAGATCCT GATAGTTTCA CAGTGCGGGC ACTACTGAAG 
GCCACGCATG AAGGCTTAAA TGCACACCTG AAGCAGAGTC CAGATGTAAA GGAAAGTGGA 
AAGAAATCAC ATGGACGATC CCTCATGACC AACTTTGGAA AACATAAGAA AACTACAAAA 
TCACGGTCTA AATCTTACAG TACTGATGAT GAGGAAGACA CACAGCAGAG TACTGGCAAG 
GAGGGTGGCC AGCTGTACAG ATTGGGTCGC CGAAGGAAAA CCATGAAGCT CTGCCGAGAA 
CTCTCTGATT TGGTTGTGTA CACAAACTCC GTGGCCGCTC AGGACATTGT GGATGACGGA 
ACCACAGGAA ATGTGTTATC ATTCAGTGAA ACAAGAGCAC ATCAGGTTGT TCAGCAAAAA 
TCAGAGCAGT TCATGATTTA TAATCAAAAG CAACTCACGA GGATTTACCC CTCTGCCTAC 
CGCATTGATT CCAGTAACTT CAACCCTCTC CCCTACTGGA ACGCAGGCTG CCAGCTAGTG 
GCACTGAATT ATCAATCTGA AGGACGAATG ATGCAGTTAA ACCGAGCCAA ATTCAAGGCA 
AATGGCAATT GTGGCTATGT CCTCAAACCC CAGCAAATGT GCAAAGGTAC TTTCAACCCT 
TTCTCTGGTG ACCCTCTTCC TGCCAACCCC AAAAAGCAGC TCATCCTGAA AGTTATCAGT 
GGACAGCAAC TCCCCAAACC TCCAGACTCC ATGTTTGGAG ATCGAGGCGA GATCATTGAC 
CCTTTTGTTG AAGTTGAAAT TATTGGATTG CCAGTAGATT GTTGTAAAGA TCAAACCCGT 
GTGGTAGATG ACAATGGATT TAACCCTGTG TGGGAAGAAA CACTGACATT TACAGTACAC 
ATGCCAGAAA TAGCTTTGGT TCGGTTCCTT GTGTGGGATC ACGATCCCAT TGGACGAGAC 
TTTGTTGGAC AAAGAACTGT GACCTTCAGC AGCTTAGTGC CTGGCTACCG GCATGTCTAT 
TTGGAAGGAC TGACAGAAGC ATCCATATTT GTACACATAA CCATCAATGA AATCTATGGA 
AAGAACAGAC AACTCCAGGG TCTGAAGGGA CTGTTCAATA AGAATCCTAG GCACAGTTCT 
TCAGAAAACA ATTCCCATTA TGTACGGAAG CGATCCATTG GAGATAGAAT TCTGCGACGC 
ACAGCTAGCG CCCCAGCCAA AGGCAGGAAA AAGAGCAAAA TGGGCTTCCA AGAAATGGTG 
GAGATAAAGG ATTCTGTGTC CGAGGCCACA AGAGATCAAG ATGGCGTGCT GAGGAGGACC 
ACACGCAGTT TGCAAGCACG CCCTGTCTCT ATGCCTGTTG ACAGAAACCT TCTGGGAGCT 
TTGTCGCTGC CTGTATCTGA AACAGCAAAA GACATTGAAG GAAAAGAAAA CTCTCTAGCA 
GAAGATAAAG ATGGCAGAAG AAAAGGGAAA GCAAGTATAA AAGATCCACA TTTTCTAAAT 
TTCAACAAAA AGTTATCATC CTCCTCCAGT GCTCTGCTCC ACAAAGATAC CAGCCAAGGG 
GACACCATTG TATCTACTGC CCACATGTCA GTCACAGGAG AACAGCTGGG CATGTCAAGT 
CCTAGGGGTG GGAGAACCAC ATCAAATGCC ACAAGCAATT GCCAGGAAAA CCCCTGTCCC 
AGCAAGTCTC TCTCCCCAAA GCAGCATTTG GCTCCCGATC CTGTAGTTAA CCCCACACAA 
GATCTGCATG GTGTGAAAAT CAAGGAAAAG GGTAATCCTG AGGACTTTGT GGAAGGGAAA 
AGCATCTTGT CAGGAAGCGT CCTTTCTCAT AGCAACCTAG AAATTAAGAA CCTGGAAGGT 
AATAGGGGTA AGGGCCGAGC TGCAACATCC TTTTCTTTGT CAGACGTCTC CATGCTCTGT 
TCTGACATAC CTGACCTACA TTCAACTGCA ATTCTGCAGG AGAGTGTAAT TTCCCATCTT 
ATTGACAATG TCACTTTAAC AAATGAGAAT GAGCCGGGCA GTTCCATCTC AGCCCTGATT 
GGCCAGTTTG ATGAGACCAA CAATCAGGCT CTCACAGTTG TTTCTCATCT TCATAATACC 
AGTGTGATGT CAGGCCATTG TCCCTTGCCT AGCCTGGGCC TAAAAATGCC CATCAAGCAT 
GGTTTTTGCA AGGGAAAATC CAAGTCTTCC TTCCTGTGCT CATCTCCGGA GCTGATAGCA 
CTCTCGAGTT CTGAGACCAC CAAACATGCA ACGAACACAG TTTATGAAAC TACCTGCACT 
CCCATCTCTA AAACCAAACC AGATGATGAC CTTTCTAGTA AGGCCAAGAC AGCGGCCTTA 
GAAAGCAACC TGCCTGGATC CCCTAATACT TCTCGTGGCT GGTTACCAAA AAGTCCTACC 
AAGGGAGAAG ACTGGGAAAC ACTGAAGAGC TGCAGCCCTG CCTCTTCCCC TGATTTGACC 
CTGGAGGATG TAATAGCTGA TCCCACTCTC TGTTTCAATT CTGGGGAGAG CAGCCTTGTG 
GAAATTGATG GAGAATCAGA AAATCTTTCT CTAACAACCT GTGAATATAG GAGAGAGGGC 
ACAAGTCAAC TTGCTTCTCC TTTAAAACTC AAGTACAATC AGGGTGTGGT AGAACACTTT 
CAAAGAGGTT TGAGAAACGG CTACTGTAAA GAGACCCTCC GCCCTTCTGT CCCTGAAATA 
TTCAACAATA TTCAAGATGT CAAAACTCAA AGTATTTCTT ATCTAGCCTA TCAGGGTGCT 
GGCTTTGTGC ATAATCATTT CTCAGATTCA GATGCAAAAA TGTTCCAGAC CTGTGTGCCC 
CAGCAATCTA GTGCTCAAGA TATGCATGTC CCTGTACCCA AGCAGTTGGC ACATCTTCCT 
TTGCCTGCTC TGAAACTGCC TAGTCCTTGC AAATCCAAAA GTCTGGGGGA CTTAACATCA 
GAGGACATTG CCTGCAATTT TGAGAGCAAG TACCAGTGTA TTAGTAAGAG TTTTGTTACA 
ACTGGCATTA GAGACAAGAA GGGCGTGACT GTGAAGACAA AGTCGTTAGA GCCTATAGAT 
GCCCTGACCG AGCAGCTTCG GAAGCTTGTG TCCTTTGACC AGGAAGACAA CTGCCAAGTG 
CTATATTCAA AGCAGGATGC CAATCAGCTC CCCCGGGCAC TGGTCAGGAA GTTGTCATCC 
AGAAGTCAGA GCAGAGTGCG CAATATTGCT AGTCGTGCCA AGGAGAAACA GGAAGCCAAC 
AAGCAGAAAG TTCCAAACCC CAGCAATGGG GCAGGAGTGG TTCTTAGAAA CAAACCCTCA 
GCACCCACCC CTGCAGTGAA TCGCCACTCC ACCGGCTCCT ACATCGCAGG CTACCTGAAG 
AACACGAAAG GGGGTGGCCT TGAAGGCCGG GGCATCCCAG AGGGGGCATG CACGGCTCTT 
CACTATGGCC ACGTTGACCA GTTTTGTTCA GATAATTCTG TTTTGCAGAC TGAGCCAAGC 
AGTGATGATA AACCAGAAAT TTATTTTCTT TTGAGACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512565.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510867.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

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CloneID	OHu65293
Clone ID Related Accession (Same CDS sequence)	XM_011512565.2 , XM_017005926.1 , XM_011512566.2
Accession Version	XM_011512565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCCGG GACACAGATG ATCAAGCTGA AACGTGGAAC CAAAGGGCTT 
GTCCGCCTCT TTTACCTGGA TGAGCACCGG ACACGCCTCC GATGGCGACC CTCTAGGAAG 
AGTGAGAAGG CAAAAATACT TATTGATTCC ATTTACAAAG TGACTGAGGG CCGGCAGTCT 
GAAATATTCC ACAGACAAGC TGAGGGGAAC TTCGACCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC CCTGGACCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAAGACTC CCTTGCCAAA 
AGGCAGAGGA CCCATGACCA ATGGGTGAAG CAGACCTTTG AGGAAGCTGA TAAGAATGGT 
GACGGCTTGC TGAATATTGA AGAGATACAT CAGCTGATGC ATAAACTGAA TGTTAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCCGACA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAGTTCTG TGTTTTTTAC AAAATGATGT CTTTGAGACG AGACCTTTAT 
TTGTTACTTT TGAGCTACAG TGACAAGAAA GATCACCTAA CTGTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAATAAT GTGACAACGG ACTATTGTCT TGACATCATA 
AAGAAGTTTG AAGTTTCAGA AGAAAATAAG GTGAAAAATG TTCTTGGCAT AGAAGGCTTC 
ACGAACTTCA TGCGTAGTCC TGCCTGTGAC ATATTTAACC CATTGCACCA TGAAGTGTAC 
CAAGACATGG ATCAGCCCCT CTGCAACTAC TACATTGCTT CCTCTCACAA TACATACCTG 
ACTGGAGACC AGCTCCTTTC TCAGTCCAAA GTGGATATGT ATGCACGGGT GCTGCAAGAG 
GGCTGTCGCT GTGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTAGTACAT 
CATGGTTACA CTCTCACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CATGCCTTTG TGAAGAATGA GTTTCCTGTT ATATTGTCTA TCGAGAATCA CTGCAGTATC 
CAGCAGCAAA GGAAGATTGC TCAGTACCTG AAAGGAATAT TCGGAGACAA ACTGGACCTG 
TCATCTGTTG ATACAGGGGA GTGCAAGCAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT CACCTTGGGG ATGATGCAGA GGAAGGGGAA 
GTTTCTGATG AGGACAGTGC AGATGAAATT GAAGACGAGT GCAAATTCAA GCTCCATTAT 
AGTAATGGGA CCACTGAGCA TCAGGTGGAA TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTAAAAGAAT CTCAAATTCG AGATAAAGAA GATCCTGATA GTTTCACAGT GCGGGCACTA 
CTGAAGGCCA CGCATGAAGG CTTAAATGCA CACCTGAAGC AGAGTCCAGA TGTAAAGGAA 
AGTGGAAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGAAAACT 
ACAAAATCAC GGTCTAAATC TTACAGTACT GATGATGAGG AAGACACACA GCAGAGTACT 
GGCAAGGAGG GTGGCCAGCT GTACAGATTG GGTCGCCGAA GGAAAACCAT GAAGCTCTGC 
CGAGAACTCT CTGATTTGGT TGTGTACACA AACTCCGTGG CCGCTCAGGA CATTGTGGAT 
GACGGAACCA CAGGAAATGT GTTATCATTC AGTGAAACAA GAGCACATCA GGTTGTTCAG 
CAAAAATCAG AGCAGTTCAT GATTTATAAT CAAAAGCAAC TCACGAGGAT TTACCCCTCT 
GCCTACCGCA TTGATTCCAG TAACTTCAAC CCTCTCCCCT ACTGGAACGC AGGCTGCCAG 
CTAGTGGCAC TGAATTATCA ATCTGAAGGA CGAATGATGC AGTTAAACCG AGCCAAATTC 
AAGGCAAATG GCAATTGTGG CTATGTCCTC AAACCCCAGC AAATGTGCAA AGGTACTTTC 
AACCCTTTCT CTGGTGACCC TCTTCCTGCC AACCCCAAAA AGCAGCTCAT CCTGAAAGTT 
ATCAGTGGAC AGCAACTCCC CAAACCTCCA GACTCCATGT TTGGAGATCG AGGCGAGATC 
ATTGACCCTT TTGTTGAAGT TGAAATTATT GGATTGCCAG TAGATTGTTG TAAAGATCAA 
ACCCGTGTGG TAGATGACAA TGGATTTAAC CCTGTGTGGG AAGAAACACT GACATTTACA 
GTACACATGC CAGAAATAGC TTTGGTTCGG TTCCTTGTGT GGGATCACGA TCCCATTGGA 
CGAGACTTTG TTGGACAAAG AACTGTGACC TTCAGCAGCT TAGTGCCTGG CTACCGGCAT 
GTCTATTTGG AAGGACTGAC AGAAGCATCC ATATTTGTAC ACATAACCAT CAATGAAATC 
TATGGAAAGT GGAGCCCTTT AATACTCAAC CCCAGTTATA CTATATTGCA CTTTCTAGGA 
GCTACAAAGA ACAGACAACT CCAGGGTCTG AAGGGACTGT TCAATAAGAA TCCTAGGCAC 
AGTTCTTCAG AAAACAATTC CCATTATGTA CGGAAGCGAT CCATTGGAGA TAGAATTCTG 
CGACGCACAG CTAGCGCCCC AGCCAAAGGC AGGAAAAAGA GCAAAATGGG CTTCCAAGAA 
ATGGTGGAGA TAAAGGATTC TGTGTCCGAG GCCACAAGAG ATCAAGATGG CGTGCTGAGG 
AGGACCACAC GCAGTTTGCA AGCACGCCCT GTCTCTATGC CTGTTGACAG AAACCTTCTG 
GGAGCTTTGT CGCTGCCTGT ATCTGAAACA GCAAAAGACA TTGAAGGAAA AGAAAACTCT 
CTAGCAGAAG ATAAAGATGG CAGAAGAAAA GGGAAAGCAA GTATAAAAGA TCCACATTTT 
CTAAATTTCA ACAAAAAGTT ATCATCCTCC TCCAGTGCTC TGCTCCACAA AGATACCAGC 
CAAGGGGACA CCATTGTATC TACTGCCCAC ATGTCAGTCA CAGGAGAACA GCTGGGCATG 
TCAAGTCCTA GGGGTGGGAG AACCACATCA AATGCCACAA GCAATTGCCA GGAAAACCCC 
TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG CATTTGGCTC CCGATCCTGT AGTTAACCCC 
ACACAAGATC TGCATGGTGT GAAAATCAAG GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA 
GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT TCTCATAGCA ACCTAGAAAT TAAGAACCTG 
GAAGGTAATA GGGGTAAGGG CCGAGCTGCA ACATCCTTTT CTTTGTCAGA CGTCTCCATG 
CTCTGTTCTG ACATACCTGA CCTACATTCA ACTGCAATTC TGCAGGAGAG TGTAATTTCC 
CATCTTATTG ACAATGTCAC TTTAACAAAT GAGAATGAGC CGGGCAGTTC CATCTCAGCC 
CTGATTGGCC AGTTTGATGA GACCAACAAT CAGGCTCTCA CAGTTGTTTC TCATCTTCAT 
AATACCAGTG TGATGTCAGG CCATTGTCCC TTGCCTAGCC TGGGCCTAAA AATGCCCATC 
AAGCATGGTT TTTGCAAGGG AAAATCCAAG TCTTCCTTCC TGTGCTCATC TCCGGAGCTG 
ATAGCACTCT CGAGTTCTGA GACCACCAAA CATGCAACGA ACACAGTTTA TGAAACTACC 
TGCACTCCCA TCTCTAAAAC CAAACCAGAT GATGACCTTT CTAGTAAGGC CAAGACAGCG 
GCCTTAGAAA GCAACCTGCC TGGATCCCCT AATACTTCTC GTGGCTGGTT ACCAAAAAGT 
CCTACCAAGG GAGAAGACTG GGAAACACTG AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT 
TTGACCCTGG AGGATGTAAT AGCTGATCCC ACTCTCTGTT TCAATTCTGG GGAGAGCAGC 
CTTGTGGAAA TTGATGGAGA ATCAGAAAAT CTTTCTCTAA CAACCTGTGA ATATAGGAGA 
GAGGGCACAA GTCAACTTGC TTCTCCTTTA AAACTCAAGT ACAATCAGGG TGTGGTAGAA 
CACTTTCAAA GAGGTTTGAG AAACGGCTAC TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT 
GAAATATTCA ACAATATTCA AGATGTCAAA ACTCAAAGTA TTTCTTATCT AGCCTATCAG 
GGTGCTGGCT TTGTGCATAA TCATTTCTCA GATTCAGATG CAAAAATGTT CCAGACCTGT 
GTGCCCCAGC AATCTAGTGC TCAAGATATG CATGTCCCTG TACCCAAGCA GTTGGCACAT 
CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT CCTTGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAG AGCAAGTACC AGTGTATTAG TAAGAGTTTT 
GTTACAACTG GCATTAGAGA CAAGAAGGGC GTGACTGTGA AGACAAAGTC GTTAGAGCCT 
ATAGATGCCC TGACCGAGCA GCTTCGGAAG CTTGTGTCCT TTGACCAGGA AGACAACTGC 
CAAGTGCTAT ATTCAAAGCA GGATGCCAAT CAGCTCCCCC GGGCACTGGT CAGGAAGTTG 
TCATCCAGAA GTCAGAGCAG AGTGCGCAAT ATTGCTAGTC GTGCCAAGGA GAAACAGGAA 
GCCAACAAGC AGAAAGTTCC AAACCCCAGC AATGGGGCAG GAGTGGTTCT TAGAAACAAA 
CCCTCAGCAC CCACCCCTGC AGTGAATCGC CACTCCACCG GCTCCTACAT CGCAGGCTAC 
CTGAAGAACA CGAAAGGGGG TGGCCTTGAA GGCCGGGGCA TCCCAGAGGG GGCATGCACG 
GCTCTTCACT ATGGCCACGT TGACCAGTTT TGTTCAGATA ATTCTGTTTT GCAGACTGAG 
CCAAGCAGTG ATGATAAACC AGAAATTTAT TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510867.1
CDS	853..5880
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512565.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512565.2

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCCGG GACACAGATG ATCAAGCTGA AACGTGGAAC CAAAGGGCTT 
GTCCGCCTCT TTTACCTGGA TGAGCACCGG ACACGCCTCC GATGGCGACC CTCTAGGAAG 
AGTGAGAAGG CAAAAATACT TATTGATTCC ATTTACAAAG TGACTGAGGG CCGGCAGTCT 
GAAATATTCC ACAGACAAGC TGAGGGGAAC TTCGACCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC CCTGGACCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAAGACTC CCTTGCCAAA 
AGGCAGAGGA CCCATGACCA ATGGGTGAAG CAGACCTTTG AGGAAGCTGA TAAGAATGGT 
GACGGCTTGC TGAATATTGA AGAGATACAT CAGCTGATGC ATAAACTGAA TGTTAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCCGACA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAGTTCTG TGTTTTTTAC AAAATGATGT CTTTGAGACG AGACCTTTAT 
TTGTTACTTT TGAGCTACAG TGACAAGAAA GATCACCTAA CTGTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAATAAT GTGACAACGG ACTATTGTCT TGACATCATA 
AAGAAGTTTG AAGTTTCAGA AGAAAATAAG GTGAAAAATG TTCTTGGCAT AGAAGGCTTC 
ACGAACTTCA TGCGTAGTCC TGCCTGTGAC ATATTTAACC CATTGCACCA TGAAGTGTAC 
CAAGACATGG ATCAGCCCCT CTGCAACTAC TACATTGCTT CCTCTCACAA TACATACCTG 
ACTGGAGACC AGCTCCTTTC TCAGTCCAAA GTGGATATGT ATGCACGGGT GCTGCAAGAG 
GGCTGTCGCT GTGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTAGTACAT 
CATGGTTACA CTCTCACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CATGCCTTTG TGAAGAATGA GTTTCCTGTT ATATTGTCTA TCGAGAATCA CTGCAGTATC 
CAGCAGCAAA GGAAGATTGC TCAGTACCTG AAAGGAATAT TCGGAGACAA ACTGGACCTG 
TCATCTGTTG ATACAGGGGA GTGCAAGCAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT CACCTTGGGG ATGATGCAGA GGAAGGGGAA 
GTTTCTGATG AGGACAGTGC AGATGAAATT GAAGACGAGT GCAAATTCAA GCTCCATTAT 
AGTAATGGGA CCACTGAGCA TCAGGTGGAA TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTAAAAGAAT CTCAAATTCG AGATAAAGAA GATCCTGATA GTTTCACAGT GCGGGCACTA 
CTGAAGGCCA CGCATGAAGG CTTAAATGCA CACCTGAAGC AGAGTCCAGA TGTAAAGGAA 
AGTGGAAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGAAAACT 
ACAAAATCAC GGTCTAAATC TTACAGTACT GATGATGAGG AAGACACACA GCAGAGTACT 
GGCAAGGAGG GTGGCCAGCT GTACAGATTG GGTCGCCGAA GGAAAACCAT GAAGCTCTGC 
CGAGAACTCT CTGATTTGGT TGTGTACACA AACTCCGTGG CCGCTCAGGA CATTGTGGAT 
GACGGAACCA CAGGAAATGT GTTATCATTC AGTGAAACAA GAGCACATCA GGTTGTTCAG 
CAAAAATCAG AGCAGTTCAT GATTTATAAT CAAAAGCAAC TCACGAGGAT TTACCCCTCT 
GCCTACCGCA TTGATTCCAG TAACTTCAAC CCTCTCCCCT ACTGGAACGC AGGCTGCCAG 
CTAGTGGCAC TGAATTATCA ATCTGAAGGA CGAATGATGC AGTTAAACCG AGCCAAATTC 
AAGGCAAATG GCAATTGTGG CTATGTCCTC AAACCCCAGC AAATGTGCAA AGGTACTTTC 
AACCCTTTCT CTGGTGACCC TCTTCCTGCC AACCCCAAAA AGCAGCTCAT CCTGAAAGTT 
ATCAGTGGAC AGCAACTCCC CAAACCTCCA GACTCCATGT TTGGAGATCG AGGCGAGATC 
ATTGACCCTT TTGTTGAAGT TGAAATTATT GGATTGCCAG TAGATTGTTG TAAAGATCAA 
ACCCGTGTGG TAGATGACAA TGGATTTAAC CCTGTGTGGG AAGAAACACT GACATTTACA 
GTACACATGC CAGAAATAGC TTTGGTTCGG TTCCTTGTGT GGGATCACGA TCCCATTGGA 
CGAGACTTTG TTGGACAAAG AACTGTGACC TTCAGCAGCT TAGTGCCTGG CTACCGGCAT 
GTCTATTTGG AAGGACTGAC AGAAGCATCC ATATTTGTAC ACATAACCAT CAATGAAATC 
TATGGAAAGT GGAGCCCTTT AATACTCAAC CCCAGTTATA CTATATTGCA CTTTCTAGGA 
GCTACAAAGA ACAGACAACT CCAGGGTCTG AAGGGACTGT TCAATAAGAA TCCTAGGCAC 
AGTTCTTCAG AAAACAATTC CCATTATGTA CGGAAGCGAT CCATTGGAGA TAGAATTCTG 
CGACGCACAG CTAGCGCCCC AGCCAAAGGC AGGAAAAAGA GCAAAATGGG CTTCCAAGAA 
ATGGTGGAGA TAAAGGATTC TGTGTCCGAG GCCACAAGAG ATCAAGATGG CGTGCTGAGG 
AGGACCACAC GCAGTTTGCA AGCACGCCCT GTCTCTATGC CTGTTGACAG AAACCTTCTG 
GGAGCTTTGT CGCTGCCTGT ATCTGAAACA GCAAAAGACA TTGAAGGAAA AGAAAACTCT 
CTAGCAGAAG ATAAAGATGG CAGAAGAAAA GGGAAAGCAA GTATAAAAGA TCCACATTTT 
CTAAATTTCA ACAAAAAGTT ATCATCCTCC TCCAGTGCTC TGCTCCACAA AGATACCAGC 
CAAGGGGACA CCATTGTATC TACTGCCCAC ATGTCAGTCA CAGGAGAACA GCTGGGCATG 
TCAAGTCCTA GGGGTGGGAG AACCACATCA AATGCCACAA GCAATTGCCA GGAAAACCCC 
TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG CATTTGGCTC CCGATCCTGT AGTTAACCCC 
ACACAAGATC TGCATGGTGT GAAAATCAAG GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA 
GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT TCTCATAGCA ACCTAGAAAT TAAGAACCTG 
GAAGGTAATA GGGGTAAGGG CCGAGCTGCA ACATCCTTTT CTTTGTCAGA CGTCTCCATG 
CTCTGTTCTG ACATACCTGA CCTACATTCA ACTGCAATTC TGCAGGAGAG TGTAATTTCC 
CATCTTATTG ACAATGTCAC TTTAACAAAT GAGAATGAGC CGGGCAGTTC CATCTCAGCC 
CTGATTGGCC AGTTTGATGA GACCAACAAT CAGGCTCTCA CAGTTGTTTC TCATCTTCAT 
AATACCAGTG TGATGTCAGG CCATTGTCCC TTGCCTAGCC TGGGCCTAAA AATGCCCATC 
AAGCATGGTT TTTGCAAGGG AAAATCCAAG TCTTCCTTCC TGTGCTCATC TCCGGAGCTG 
ATAGCACTCT CGAGTTCTGA GACCACCAAA CATGCAACGA ACACAGTTTA TGAAACTACC 
TGCACTCCCA TCTCTAAAAC CAAACCAGAT GATGACCTTT CTAGTAAGGC CAAGACAGCG 
GCCTTAGAAA GCAACCTGCC TGGATCCCCT AATACTTCTC GTGGCTGGTT ACCAAAAAGT 
CCTACCAAGG GAGAAGACTG GGAAACACTG AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT 
TTGACCCTGG AGGATGTAAT AGCTGATCCC ACTCTCTGTT TCAATTCTGG GGAGAGCAGC 
CTTGTGGAAA TTGATGGAGA ATCAGAAAAT CTTTCTCTAA CAACCTGTGA ATATAGGAGA 
GAGGGCACAA GTCAACTTGC TTCTCCTTTA AAACTCAAGT ACAATCAGGG TGTGGTAGAA 
CACTTTCAAA GAGGTTTGAG AAACGGCTAC TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT 
GAAATATTCA ACAATATTCA AGATGTCAAA ACTCAAAGTA TTTCTTATCT AGCCTATCAG 
GGTGCTGGCT TTGTGCATAA TCATTTCTCA GATTCAGATG CAAAAATGTT CCAGACCTGT 
GTGCCCCAGC AATCTAGTGC TCAAGATATG CATGTCCCTG TACCCAAGCA GTTGGCACAT 
CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT CCTTGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAG AGCAAGTACC AGTGTATTAG TAAGAGTTTT 
GTTACAACTG GCATTAGAGA CAAGAAGGGC GTGACTGTGA AGACAAAGTC GTTAGAGCCT 
ATAGATGCCC TGACCGAGCA GCTTCGGAAG CTTGTGTCCT TTGACCAGGA AGACAACTGC 
CAAGTGCTAT ATTCAAAGCA GGATGCCAAT CAGCTCCCCC GGGCACTGGT CAGGAAGTTG 
TCATCCAGAA GTCAGAGCAG AGTGCGCAAT ATTGCTAGTC GTGCCAAGGA GAAACAGGAA 
GCCAACAAGC AGAAAGTTCC AAACCCCAGC AATGGGGCAG GAGTGGTTCT TAGAAACAAA 
CCCTCAGCAC CCACCCCTGC AGTGAATCGC CACTCCACCG GCTCCTACAT CGCAGGCTAC 
CTGAAGAACA CGAAAGGGGG TGGCCTTGAA GGCCGGGGCA TCCCAGAGGG GGCATGCACG 
GCTCTTCACT ATGGCCACGT TGACCAGTTT TGTTCAGATA ATTCTGTTTT GCAGACTGAG 
CCAAGCAGTG ATGATAAACC AGAAATTTAT TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_017005926.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861415.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

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CloneID	OHu65293
Clone ID Related Accession (Same CDS sequence)	XM_011512565.2 , XM_017005926.1 , XM_011512566.2
Accession Version	XM_017005926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCCGG GACACAGATG ATCAAGCTGA AACGTGGAAC CAAAGGGCTT 
GTCCGCCTCT TTTACCTGGA TGAGCACCGG ACACGCCTCC GATGGCGACC CTCTAGGAAG 
AGTGAGAAGG CAAAAATACT TATTGATTCC ATTTACAAAG TGACTGAGGG CCGGCAGTCT 
GAAATATTCC ACAGACAAGC TGAGGGGAAC TTCGACCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC CCTGGACCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAAGACTC CCTTGCCAAA 
AGGCAGAGGA CCCATGACCA ATGGGTGAAG CAGACCTTTG AGGAAGCTGA TAAGAATGGT 
GACGGCTTGC TGAATATTGA AGAGATACAT CAGCTGATGC ATAAACTGAA TGTTAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCCGACA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAGTTCTG TGTTTTTTAC AAAATGATGT CTTTGAGACG AGACCTTTAT 
TTGTTACTTT TGAGCTACAG TGACAAGAAA GATCACCTAA CTGTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAATAAT GTGACAACGG ACTATTGTCT TGACATCATA 
AAGAAGTTTG AAGTTTCAGA AGAAAATAAG GTGAAAAATG TTCTTGGCAT AGAAGGCTTC 
ACGAACTTCA TGCGTAGTCC TGCCTGTGAC ATATTTAACC CATTGCACCA TGAAGTGTAC 
CAAGACATGG ATCAGCCCCT CTGCAACTAC TACATTGCTT CCTCTCACAA TACATACCTG 
ACTGGAGACC AGCTCCTTTC TCAGTCCAAA GTGGATATGT ATGCACGGGT GCTGCAAGAG 
GGCTGTCGCT GTGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTAGTACAT 
CATGGTTACA CTCTCACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CATGCCTTTG TGAAGAATGA GTTTCCTGTT ATATTGTCTA TCGAGAATCA CTGCAGTATC 
CAGCAGCAAA GGAAGATTGC TCAGTACCTG AAAGGAATAT TCGGAGACAA ACTGGACCTG 
TCATCTGTTG ATACAGGGGA GTGCAAGCAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT CACCTTGGGG ATGATGCAGA GGAAGGGGAA 
GTTTCTGATG AGGACAGTGC AGATGAAATT GAAGACGAGT GCAAATTCAA GCTCCATTAT 
AGTAATGGGA CCACTGAGCA TCAGGTGGAA TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTAAAAGAAT CTCAAATTCG AGATAAAGAA GATCCTGATA GTTTCACAGT GCGGGCACTA 
CTGAAGGCCA CGCATGAAGG CTTAAATGCA CACCTGAAGC AGAGTCCAGA TGTAAAGGAA 
AGTGGAAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGAAAACT 
ACAAAATCAC GGTCTAAATC TTACAGTACT GATGATGAGG AAGACACACA GCAGAGTACT 
GGCAAGGAGG GTGGCCAGCT GTACAGATTG GGTCGCCGAA GGAAAACCAT GAAGCTCTGC 
CGAGAACTCT CTGATTTGGT TGTGTACACA AACTCCGTGG CCGCTCAGGA CATTGTGGAT 
GACGGAACCA CAGGAAATGT GTTATCATTC AGTGAAACAA GAGCACATCA GGTTGTTCAG 
CAAAAATCAG AGCAGTTCAT GATTTATAAT CAAAAGCAAC TCACGAGGAT TTACCCCTCT 
GCCTACCGCA TTGATTCCAG TAACTTCAAC CCTCTCCCCT ACTGGAACGC AGGCTGCCAG 
CTAGTGGCAC TGAATTATCA ATCTGAAGGA CGAATGATGC AGTTAAACCG AGCCAAATTC 
AAGGCAAATG GCAATTGTGG CTATGTCCTC AAACCCCAGC AAATGTGCAA AGGTACTTTC 
AACCCTTTCT CTGGTGACCC TCTTCCTGCC AACCCCAAAA AGCAGCTCAT CCTGAAAGTT 
ATCAGTGGAC AGCAACTCCC CAAACCTCCA GACTCCATGT TTGGAGATCG AGGCGAGATC 
ATTGACCCTT TTGTTGAAGT TGAAATTATT GGATTGCCAG TAGATTGTTG TAAAGATCAA 
ACCCGTGTGG TAGATGACAA TGGATTTAAC CCTGTGTGGG AAGAAACACT GACATTTACA 
GTACACATGC CAGAAATAGC TTTGGTTCGG TTCCTTGTGT GGGATCACGA TCCCATTGGA 
CGAGACTTTG TTGGACAAAG AACTGTGACC TTCAGCAGCT TAGTGCCTGG CTACCGGCAT 
GTCTATTTGG AAGGACTGAC AGAAGCATCC ATATTTGTAC ACATAACCAT CAATGAAATC 
TATGGAAAGT GGAGCCCTTT AATACTCAAC CCCAGTTATA CTATATTGCA CTTTCTAGGA 
GCTACAAAGA ACAGACAACT CCAGGGTCTG AAGGGACTGT TCAATAAGAA TCCTAGGCAC 
AGTTCTTCAG AAAACAATTC CCATTATGTA CGGAAGCGAT CCATTGGAGA TAGAATTCTG 
CGACGCACAG CTAGCGCCCC AGCCAAAGGC AGGAAAAAGA GCAAAATGGG CTTCCAAGAA 
ATGGTGGAGA TAAAGGATTC TGTGTCCGAG GCCACAAGAG ATCAAGATGG CGTGCTGAGG 
AGGACCACAC GCAGTTTGCA AGCACGCCCT GTCTCTATGC CTGTTGACAG AAACCTTCTG 
GGAGCTTTGT CGCTGCCTGT ATCTGAAACA GCAAAAGACA TTGAAGGAAA AGAAAACTCT 
CTAGCAGAAG ATAAAGATGG CAGAAGAAAA GGGAAAGCAA GTATAAAAGA TCCACATTTT 
CTAAATTTCA ACAAAAAGTT ATCATCCTCC TCCAGTGCTC TGCTCCACAA AGATACCAGC 
CAAGGGGACA CCATTGTATC TACTGCCCAC ATGTCAGTCA CAGGAGAACA GCTGGGCATG 
TCAAGTCCTA GGGGTGGGAG AACCACATCA AATGCCACAA GCAATTGCCA GGAAAACCCC 
TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG CATTTGGCTC CCGATCCTGT AGTTAACCCC 
ACACAAGATC TGCATGGTGT GAAAATCAAG GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA 
GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT TCTCATAGCA ACCTAGAAAT TAAGAACCTG 
GAAGGTAATA GGGGTAAGGG CCGAGCTGCA ACATCCTTTT CTTTGTCAGA CGTCTCCATG 
CTCTGTTCTG ACATACCTGA CCTACATTCA ACTGCAATTC TGCAGGAGAG TGTAATTTCC 
CATCTTATTG ACAATGTCAC TTTAACAAAT GAGAATGAGC CGGGCAGTTC CATCTCAGCC 
CTGATTGGCC AGTTTGATGA GACCAACAAT CAGGCTCTCA CAGTTGTTTC TCATCTTCAT 
AATACCAGTG TGATGTCAGG CCATTGTCCC TTGCCTAGCC TGGGCCTAAA AATGCCCATC 
AAGCATGGTT TTTGCAAGGG AAAATCCAAG TCTTCCTTCC TGTGCTCATC TCCGGAGCTG 
ATAGCACTCT CGAGTTCTGA GACCACCAAA CATGCAACGA ACACAGTTTA TGAAACTACC 
TGCACTCCCA TCTCTAAAAC CAAACCAGAT GATGACCTTT CTAGTAAGGC CAAGACAGCG 
GCCTTAGAAA GCAACCTGCC TGGATCCCCT AATACTTCTC GTGGCTGGTT ACCAAAAAGT 
CCTACCAAGG GAGAAGACTG GGAAACACTG AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT 
TTGACCCTGG AGGATGTAAT AGCTGATCCC ACTCTCTGTT TCAATTCTGG GGAGAGCAGC 
CTTGTGGAAA TTGATGGAGA ATCAGAAAAT CTTTCTCTAA CAACCTGTGA ATATAGGAGA 
GAGGGCACAA GTCAACTTGC TTCTCCTTTA AAACTCAAGT ACAATCAGGG TGTGGTAGAA 
CACTTTCAAA GAGGTTTGAG AAACGGCTAC TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT 
GAAATATTCA ACAATATTCA AGATGTCAAA ACTCAAAGTA TTTCTTATCT AGCCTATCAG 
GGTGCTGGCT TTGTGCATAA TCATTTCTCA GATTCAGATG CAAAAATGTT CCAGACCTGT 
GTGCCCCAGC AATCTAGTGC TCAAGATATG CATGTCCCTG TACCCAAGCA GTTGGCACAT 
CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT CCTTGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAG AGCAAGTACC AGTGTATTAG TAAGAGTTTT 
GTTACAACTG GCATTAGAGA CAAGAAGGGC GTGACTGTGA AGACAAAGTC GTTAGAGCCT 
ATAGATGCCC TGACCGAGCA GCTTCGGAAG CTTGTGTCCT TTGACCAGGA AGACAACTGC 
CAAGTGCTAT ATTCAAAGCA GGATGCCAAT CAGCTCCCCC GGGCACTGGT CAGGAAGTTG 
TCATCCAGAA GTCAGAGCAG AGTGCGCAAT ATTGCTAGTC GTGCCAAGGA GAAACAGGAA 
GCCAACAAGC AGAAAGTTCC AAACCCCAGC AATGGGGCAG GAGTGGTTCT TAGAAACAAA 
CCCTCAGCAC CCACCCCTGC AGTGAATCGC CACTCCACCG GCTCCTACAT CGCAGGCTAC 
CTGAAGAACA CGAAAGGGGG TGGCCTTGAA GGCCGGGGCA TCCCAGAGGG GGCATGCACG 
GCTCTTCACT ATGGCCACGT TGACCAGTTT TGTTCAGATA ATTCTGTTTT GCAGACTGAG 
CCAAGCAGTG ATGATAAACC AGAAATTTAT TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861415.1
CDS	280..5307
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_017005926.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005926.1

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCCGG GACACAGATG ATCAAGCTGA AACGTGGAAC CAAAGGGCTT 
GTCCGCCTCT TTTACCTGGA TGAGCACCGG ACACGCCTCC GATGGCGACC CTCTAGGAAG 
AGTGAGAAGG CAAAAATACT TATTGATTCC ATTTACAAAG TGACTGAGGG CCGGCAGTCT 
GAAATATTCC ACAGACAAGC TGAGGGGAAC TTCGACCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC CCTGGACCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAAGACTC CCTTGCCAAA 
AGGCAGAGGA CCCATGACCA ATGGGTGAAG CAGACCTTTG AGGAAGCTGA TAAGAATGGT 
GACGGCTTGC TGAATATTGA AGAGATACAT CAGCTGATGC ATAAACTGAA TGTTAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCCGACA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAGTTCTG TGTTTTTTAC AAAATGATGT CTTTGAGACG AGACCTTTAT 
TTGTTACTTT TGAGCTACAG TGACAAGAAA GATCACCTAA CTGTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAATAAT GTGACAACGG ACTATTGTCT TGACATCATA 
AAGAAGTTTG AAGTTTCAGA AGAAAATAAG GTGAAAAATG TTCTTGGCAT AGAAGGCTTC 
ACGAACTTCA TGCGTAGTCC TGCCTGTGAC ATATTTAACC CATTGCACCA TGAAGTGTAC 
CAAGACATGG ATCAGCCCCT CTGCAACTAC TACATTGCTT CCTCTCACAA TACATACCTG 
ACTGGAGACC AGCTCCTTTC TCAGTCCAAA GTGGATATGT ATGCACGGGT GCTGCAAGAG 
GGCTGTCGCT GTGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTAGTACAT 
CATGGTTACA CTCTCACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CATGCCTTTG TGAAGAATGA GTTTCCTGTT ATATTGTCTA TCGAGAATCA CTGCAGTATC 
CAGCAGCAAA GGAAGATTGC TCAGTACCTG AAAGGAATAT TCGGAGACAA ACTGGACCTG 
TCATCTGTTG ATACAGGGGA GTGCAAGCAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT CACCTTGGGG ATGATGCAGA GGAAGGGGAA 
GTTTCTGATG AGGACAGTGC AGATGAAATT GAAGACGAGT GCAAATTCAA GCTCCATTAT 
AGTAATGGGA CCACTGAGCA TCAGGTGGAA TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTAAAAGAAT CTCAAATTCG AGATAAAGAA GATCCTGATA GTTTCACAGT GCGGGCACTA 
CTGAAGGCCA CGCATGAAGG CTTAAATGCA CACCTGAAGC AGAGTCCAGA TGTAAAGGAA 
AGTGGAAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGAAAACT 
ACAAAATCAC GGTCTAAATC TTACAGTACT GATGATGAGG AAGACACACA GCAGAGTACT 
GGCAAGGAGG GTGGCCAGCT GTACAGATTG GGTCGCCGAA GGAAAACCAT GAAGCTCTGC 
CGAGAACTCT CTGATTTGGT TGTGTACACA AACTCCGTGG CCGCTCAGGA CATTGTGGAT 
GACGGAACCA CAGGAAATGT GTTATCATTC AGTGAAACAA GAGCACATCA GGTTGTTCAG 
CAAAAATCAG AGCAGTTCAT GATTTATAAT CAAAAGCAAC TCACGAGGAT TTACCCCTCT 
GCCTACCGCA TTGATTCCAG TAACTTCAAC CCTCTCCCCT ACTGGAACGC AGGCTGCCAG 
CTAGTGGCAC TGAATTATCA ATCTGAAGGA CGAATGATGC AGTTAAACCG AGCCAAATTC 
AAGGCAAATG GCAATTGTGG CTATGTCCTC AAACCCCAGC AAATGTGCAA AGGTACTTTC 
AACCCTTTCT CTGGTGACCC TCTTCCTGCC AACCCCAAAA AGCAGCTCAT CCTGAAAGTT 
ATCAGTGGAC AGCAACTCCC CAAACCTCCA GACTCCATGT TTGGAGATCG AGGCGAGATC 
ATTGACCCTT TTGTTGAAGT TGAAATTATT GGATTGCCAG TAGATTGTTG TAAAGATCAA 
ACCCGTGTGG TAGATGACAA TGGATTTAAC CCTGTGTGGG AAGAAACACT GACATTTACA 
GTACACATGC CAGAAATAGC TTTGGTTCGG TTCCTTGTGT GGGATCACGA TCCCATTGGA 
CGAGACTTTG TTGGACAAAG AACTGTGACC TTCAGCAGCT TAGTGCCTGG CTACCGGCAT 
GTCTATTTGG AAGGACTGAC AGAAGCATCC ATATTTGTAC ACATAACCAT CAATGAAATC 
TATGGAAAGT GGAGCCCTTT AATACTCAAC CCCAGTTATA CTATATTGCA CTTTCTAGGA 
GCTACAAAGA ACAGACAACT CCAGGGTCTG AAGGGACTGT TCAATAAGAA TCCTAGGCAC 
AGTTCTTCAG AAAACAATTC CCATTATGTA CGGAAGCGAT CCATTGGAGA TAGAATTCTG 
CGACGCACAG CTAGCGCCCC AGCCAAAGGC AGGAAAAAGA GCAAAATGGG CTTCCAAGAA 
ATGGTGGAGA TAAAGGATTC TGTGTCCGAG GCCACAAGAG ATCAAGATGG CGTGCTGAGG 
AGGACCACAC GCAGTTTGCA AGCACGCCCT GTCTCTATGC CTGTTGACAG AAACCTTCTG 
GGAGCTTTGT CGCTGCCTGT ATCTGAAACA GCAAAAGACA TTGAAGGAAA AGAAAACTCT 
CTAGCAGAAG ATAAAGATGG CAGAAGAAAA GGGAAAGCAA GTATAAAAGA TCCACATTTT 
CTAAATTTCA ACAAAAAGTT ATCATCCTCC TCCAGTGCTC TGCTCCACAA AGATACCAGC 
CAAGGGGACA CCATTGTATC TACTGCCCAC ATGTCAGTCA CAGGAGAACA GCTGGGCATG 
TCAAGTCCTA GGGGTGGGAG AACCACATCA AATGCCACAA GCAATTGCCA GGAAAACCCC 
TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG CATTTGGCTC CCGATCCTGT AGTTAACCCC 
ACACAAGATC TGCATGGTGT GAAAATCAAG GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA 
GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT TCTCATAGCA ACCTAGAAAT TAAGAACCTG 
GAAGGTAATA GGGGTAAGGG CCGAGCTGCA ACATCCTTTT CTTTGTCAGA CGTCTCCATG 
CTCTGTTCTG ACATACCTGA CCTACATTCA ACTGCAATTC TGCAGGAGAG TGTAATTTCC 
CATCTTATTG ACAATGTCAC TTTAACAAAT GAGAATGAGC CGGGCAGTTC CATCTCAGCC 
CTGATTGGCC AGTTTGATGA GACCAACAAT CAGGCTCTCA CAGTTGTTTC TCATCTTCAT 
AATACCAGTG TGATGTCAGG CCATTGTCCC TTGCCTAGCC TGGGCCTAAA AATGCCCATC 
AAGCATGGTT TTTGCAAGGG AAAATCCAAG TCTTCCTTCC TGTGCTCATC TCCGGAGCTG 
ATAGCACTCT CGAGTTCTGA GACCACCAAA CATGCAACGA ACACAGTTTA TGAAACTACC 
TGCACTCCCA TCTCTAAAAC CAAACCAGAT GATGACCTTT CTAGTAAGGC CAAGACAGCG 
GCCTTAGAAA GCAACCTGCC TGGATCCCCT AATACTTCTC GTGGCTGGTT ACCAAAAAGT 
CCTACCAAGG GAGAAGACTG GGAAACACTG AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT 
TTGACCCTGG AGGATGTAAT AGCTGATCCC ACTCTCTGTT TCAATTCTGG GGAGAGCAGC 
CTTGTGGAAA TTGATGGAGA ATCAGAAAAT CTTTCTCTAA CAACCTGTGA ATATAGGAGA 
GAGGGCACAA GTCAACTTGC TTCTCCTTTA AAACTCAAGT ACAATCAGGG TGTGGTAGAA 
CACTTTCAAA GAGGTTTGAG AAACGGCTAC TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT 
GAAATATTCA ACAATATTCA AGATGTCAAA ACTCAAAGTA TTTCTTATCT AGCCTATCAG 
GGTGCTGGCT TTGTGCATAA TCATTTCTCA GATTCAGATG CAAAAATGTT CCAGACCTGT 
GTGCCCCAGC AATCTAGTGC TCAAGATATG CATGTCCCTG TACCCAAGCA GTTGGCACAT 
CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT CCTTGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAG AGCAAGTACC AGTGTATTAG TAAGAGTTTT 
GTTACAACTG GCATTAGAGA CAAGAAGGGC GTGACTGTGA AGACAAAGTC GTTAGAGCCT 
ATAGATGCCC TGACCGAGCA GCTTCGGAAG CTTGTGTCCT TTGACCAGGA AGACAACTGC 
CAAGTGCTAT ATTCAAAGCA GGATGCCAAT CAGCTCCCCC GGGCACTGGT CAGGAAGTTG 
TCATCCAGAA GTCAGAGCAG AGTGCGCAAT ATTGCTAGTC GTGCCAAGGA GAAACAGGAA 
GCCAACAAGC AGAAAGTTCC AAACCCCAGC AATGGGGCAG GAGTGGTTCT TAGAAACAAA 
CCCTCAGCAC CCACCCCTGC AGTGAATCGC CACTCCACCG GCTCCTACAT CGCAGGCTAC 
CTGAAGAACA CGAAAGGGGG TGGCCTTGAA GGCCGGGGCA TCCCAGAGGG GGCATGCACG 
GCTCTTCACT ATGGCCACGT TGACCAGTTT TGTTCAGATA ATTCTGTTTT GCAGACTGAG 
CCAAGCAGTG ATGATAAACC AGAAATTTAT TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCH1 ( XM_011512566.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510868.1 Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X10, mRNA.

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CloneID	OHu65293
Clone ID Related Accession (Same CDS sequence)	XM_011512565.2 , XM_017005926.1 , XM_011512566.2
Accession Version	XM_011512566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA TGCAGTCCGG GACACAGATG ATCAAGCTGA AACGTGGAAC CAAAGGGCTT 
GTCCGCCTCT TTTACCTGGA TGAGCACCGG ACACGCCTCC GATGGCGACC CTCTAGGAAG 
AGTGAGAAGG CAAAAATACT TATTGATTCC ATTTACAAAG TGACTGAGGG CCGGCAGTCT 
GAAATATTCC ACAGACAAGC TGAGGGGAAC TTCGACCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC CCTGGACCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAAGACTC CCTTGCCAAA 
AGGCAGAGGA CCCATGACCA ATGGGTGAAG CAGACCTTTG AGGAAGCTGA TAAGAATGGT 
GACGGCTTGC TGAATATTGA AGAGATACAT CAGCTGATGC ATAAACTGAA TGTTAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCCGACA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAGTTCTG TGTTTTTTAC AAAATGATGT CTTTGAGACG AGACCTTTAT 
TTGTTACTTT TGAGCTACAG TGACAAGAAA GATCACCTAA CTGTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAATAAT GTGACAACGG ACTATTGTCT TGACATCATA 
AAGAAGTTTG AAGTTTCAGA AGAAAATAAG GTGAAAAATG TTCTTGGCAT AGAAGGCTTC 
ACGAACTTCA TGCGTAGTCC TGCCTGTGAC ATATTTAACC CATTGCACCA TGAAGTGTAC 
CAAGACATGG ATCAGCCCCT CTGCAACTAC TACATTGCTT CCTCTCACAA TACATACCTG 
ACTGGAGACC AGCTCCTTTC TCAGTCCAAA GTGGATATGT ATGCACGGGT GCTGCAAGAG 
GGCTGTCGCT GTGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTAGTACAT 
CATGGTTACA CTCTCACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CATGCCTTTG TGAAGAATGA GTTTCCTGTT ATATTGTCTA TCGAGAATCA CTGCAGTATC 
CAGCAGCAAA GGAAGATTGC TCAGTACCTG AAAGGAATAT TCGGAGACAA ACTGGACCTG 
TCATCTGTTG ATACAGGGGA GTGCAAGCAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT CACCTTGGGG ATGATGCAGA GGAAGGGGAA 
GTTTCTGATG AGGACAGTGC AGATGAAATT GAAGACGAGT GCAAATTCAA GCTCCATTAT 
AGTAATGGGA CCACTGAGCA TCAGGTGGAA TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTAAAAGAAT CTCAAATTCG AGATAAAGAA GATCCTGATA GTTTCACAGT GCGGGCACTA 
CTGAAGGCCA CGCATGAAGG CTTAAATGCA CACCTGAAGC AGAGTCCAGA TGTAAAGGAA 
AGTGGAAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGAAAACT 
ACAAAATCAC GGTCTAAATC TTACAGTACT GATGATGAGG AAGACACACA GCAGAGTACT 
GGCAAGGAGG GTGGCCAGCT GTACAGATTG GGTCGCCGAA GGAAAACCAT GAAGCTCTGC 
CGAGAACTCT CTGATTTGGT TGTGTACACA AACTCCGTGG CCGCTCAGGA CATTGTGGAT 
GACGGAACCA CAGGAAATGT GTTATCATTC AGTGAAACAA GAGCACATCA GGTTGTTCAG 
CAAAAATCAG AGCAGTTCAT GATTTATAAT CAAAAGCAAC TCACGAGGAT TTACCCCTCT 
GCCTACCGCA TTGATTCCAG TAACTTCAAC CCTCTCCCCT ACTGGAACGC AGGCTGCCAG 
CTAGTGGCAC TGAATTATCA ATCTGAAGGA CGAATGATGC AGTTAAACCG AGCCAAATTC 
AAGGCAAATG GCAATTGTGG CTATGTCCTC AAACCCCAGC AAATGTGCAA AGGTACTTTC 
AACCCTTTCT CTGGTGACCC TCTTCCTGCC AACCCCAAAA AGCAGCTCAT CCTGAAAGTT 
ATCAGTGGAC AGCAACTCCC CAAACCTCCA GACTCCATGT TTGGAGATCG AGGCGAGATC 
ATTGACCCTT TTGTTGAAGT TGAAATTATT GGATTGCCAG TAGATTGTTG TAAAGATCAA 
ACCCGTGTGG TAGATGACAA TGGATTTAAC CCTGTGTGGG AAGAAACACT GACATTTACA 
GTACACATGC CAGAAATAGC TTTGGTTCGG TTCCTTGTGT GGGATCACGA TCCCATTGGA 
CGAGACTTTG TTGGACAAAG AACTGTGACC TTCAGCAGCT TAGTGCCTGG CTACCGGCAT 
GTCTATTTGG AAGGACTGAC AGAAGCATCC ATATTTGTAC ACATAACCAT CAATGAAATC 
TATGGAAAGT GGAGCCCTTT AATACTCAAC CCCAGTTATA CTATATTGCA CTTTCTAGGA 
GCTACAAAGA ACAGACAACT CCAGGGTCTG AAGGGACTGT TCAATAAGAA TCCTAGGCAC 
AGTTCTTCAG AAAACAATTC CCATTATGTA CGGAAGCGAT CCATTGGAGA TAGAATTCTG 
CGACGCACAG CTAGCGCCCC AGCCAAAGGC AGGAAAAAGA GCAAAATGGG CTTCCAAGAA 
ATGGTGGAGA TAAAGGATTC TGTGTCCGAG GCCACAAGAG ATCAAGATGG CGTGCTGAGG 
AGGACCACAC GCAGTTTGCA AGCACGCCCT GTCTCTATGC CTGTTGACAG AAACCTTCTG 
GGAGCTTTGT CGCTGCCTGT ATCTGAAACA GCAAAAGACA TTGAAGGAAA AGAAAACTCT 
CTAGCAGAAG ATAAAGATGG CAGAAGAAAA GGGAAAGCAA GTATAAAAGA TCCACATTTT 
CTAAATTTCA ACAAAAAGTT ATCATCCTCC TCCAGTGCTC TGCTCCACAA AGATACCAGC 
CAAGGGGACA CCATTGTATC TACTGCCCAC ATGTCAGTCA CAGGAGAACA GCTGGGCATG 
TCAAGTCCTA GGGGTGGGAG AACCACATCA AATGCCACAA GCAATTGCCA GGAAAACCCC 
TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG CATTTGGCTC CCGATCCTGT AGTTAACCCC 
ACACAAGATC TGCATGGTGT GAAAATCAAG GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA 
GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT TCTCATAGCA ACCTAGAAAT TAAGAACCTG 
GAAGGTAATA GGGGTAAGGG CCGAGCTGCA ACATCCTTTT CTTTGTCAGA CGTCTCCATG 
CTCTGTTCTG ACATACCTGA CCTACATTCA ACTGCAATTC TGCAGGAGAG TGTAATTTCC 
CATCTTATTG ACAATGTCAC TTTAACAAAT GAGAATGAGC CGGGCAGTTC CATCTCAGCC 
CTGATTGGCC AGTTTGATGA GACCAACAAT CAGGCTCTCA CAGTTGTTTC TCATCTTCAT 
AATACCAGTG TGATGTCAGG CCATTGTCCC TTGCCTAGCC TGGGCCTAAA AATGCCCATC 
AAGCATGGTT TTTGCAAGGG AAAATCCAAG TCTTCCTTCC TGTGCTCATC TCCGGAGCTG 
ATAGCACTCT CGAGTTCTGA GACCACCAAA CATGCAACGA ACACAGTTTA TGAAACTACC 
TGCACTCCCA TCTCTAAAAC CAAACCAGAT GATGACCTTT CTAGTAAGGC CAAGACAGCG 
GCCTTAGAAA GCAACCTGCC TGGATCCCCT AATACTTCTC GTGGCTGGTT ACCAAAAAGT 
CCTACCAAGG GAGAAGACTG GGAAACACTG AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT 
TTGACCCTGG AGGATGTAAT AGCTGATCCC ACTCTCTGTT TCAATTCTGG GGAGAGCAGC 
CTTGTGGAAA TTGATGGAGA ATCAGAAAAT CTTTCTCTAA CAACCTGTGA ATATAGGAGA 
GAGGGCACAA GTCAACTTGC TTCTCCTTTA AAACTCAAGT ACAATCAGGG TGTGGTAGAA 
CACTTTCAAA GAGGTTTGAG AAACGGCTAC TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT 
GAAATATTCA ACAATATTCA AGATGTCAAA ACTCAAAGTA TTTCTTATCT AGCCTATCAG 
GGTGCTGGCT TTGTGCATAA TCATTTCTCA GATTCAGATG CAAAAATGTT CCAGACCTGT 
GTGCCCCAGC AATCTAGTGC TCAAGATATG CATGTCCCTG TACCCAAGCA GTTGGCACAT 
CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT CCTTGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAG AGCAAGTACC AGTGTATTAG TAAGAGTTTT 
GTTACAACTG GCATTAGAGA CAAGAAGGGC GTGACTGTGA AGACAAAGTC GTTAGAGCCT 
ATAGATGCCC TGACCGAGCA GCTTCGGAAG CTTGTGTCCT TTGACCAGGA AGACAACTGC 
CAAGTGCTAT ATTCAAAGCA GGATGCCAAT CAGCTCCCCC GGGCACTGGT CAGGAAGTTG 
TCATCCAGAA GTCAGAGCAG AGTGCGCAAT ATTGCTAGTC GTGCCAAGGA GAAACAGGAA 
GCCAACAAGC AGAAAGTTCC AAACCCCAGC AATGGGGCAG GAGTGGTTCT TAGAAACAAA 
CCCTCAGCAC CCACCCCTGC AGTGAATCGC CACTCCACCG GCTCCTACAT CGCAGGCTAC 
CTGAAGAACA CGAAAGGGGG TGGCCTTGAA GGCCGGGGCA TCCCAGAGGG GGCATGCACG 
GCTCTTCACT ATGGCCACGT TGACCAGTTT TGTTCAGATA ATTCTGTTTT GCAGACTGAG 
CCAAGCAGTG ATGATAAACC AGAAATTTAT TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510868.1
CDS	494..5521
Translation	MSVMQSGTQMIKLKRGTKGLVRLFYLDEHRTRLRWRPSRKSEKAKILIDSIYKVTEGRQSEIFHRQAEGNFDPSCCFTIYHGNHMESLDLITSNPEEARTWITGLKYLMAGISDEDSLAKRQRTHDQWVKQTFEEADKNGDGLLNIEEIHQLMHKLNVNLPRRKVRQMFQEADTDENQGTLTFEEFCVFYKMMSLRRDLYLLLLSYSDKKDHLTVEELAQFLKVEQKMNNVTTDYCLDIIKKFEVSEENKVKNVLGIEGFTNFMRSPACDIFNPLHHEVYQDMDQPLCNYYIASSHNTYLTGDQLLSQSKVDMYARVLQEGCRCVEVDCWDGPDGEPVVHHGYTLTSKILFRDVVETINKHAFVKNEFPVILSIENHCSIQQQRKIAQYLKGIFGDKLDLSSVDTGECKQLPSPQSLKGKILVKGKKLPYHLGDDAEEGEVSDEDSADEIEDECKFKLHYSNGTTEHQVESFIRKKLESLLKESQIRDKEDPDSFTVRALLKATHEGLNAHLKQSPDVKESGKKSHGRSLMTNFGKHKKTTKSRSKSYSTDDEEDTQQSTGKEGGQLYRLGRRRKTMKLCRELSDLVVYTNSVAAQDIVDDGTTGNVLSFSETRAHQVVQQKSEQFMIYNQKQLTRIYPSAYRIDSSNFNPLPYWNAGCQLVALNYQSEGRMMQLNRAKFKANGNCGYVLKPQQMCKGTFNPFSGDPLPANPKKQLILKVISGQQLPKPPDSMFGDRGEIIDPFVEVEIIGLPVDCCKDQTRVVDDNGFNPVWEETLTFTVHMPEIALVRFLVWDHDPIGRDFVGQRTVTFSSLVPGYRHVYLEGLTEASIFVHITINEIYGKWSPLILNPSYTILHFLGATKNRQLQGLKGLFNKNPRHSSSENNSHYVRKRSIGDRILRRTASAPAKGRKKSKMGFQEMVEIKDSVSEATRDQDGVLRRTTRSLQARPVSMPVDRNLLGALSLPVSETAKDIEGKENSLAEDKDGRRKGKASIKDPHFLNFNKKLSSSSSALLHKDTSQGDTIVSTAHMSVTGEQLGMSSPRGGRTTSNATSNCQENPCPSKSLSPKQHLAPDPVVNPTQDLHGVKIKEKGNPEDFVEGKSILSGSVLSHSNLEIKNLEGNRGKGRAATSFSLSDVSMLCSDIPDLHSTAILQESVISHLIDNVTLTNENEPGSSISALIGQFDETNNQALTVVSHLHNTSVMSGHCPLPSLGLKMPIKHGFCKGKSKSSFLCSSPELIALSSSETTKHATNTVYETTCTPISKTKPDDDLSSKAKTAALESNLPGSPNTSRGWLPKSPTKGEDWETLKSCSPASSPDLTLEDVIADPTLCFNSGESSLVEIDGESENLSLTTCEYRREGTSQLASPLKLKYNQGVVEHFQRGLRNGYCKETLRPSVPEIFNNIQDVKTQSISYLAYQGAGFVHNHFSDSDAKMFQTCVPQQSSAQDMHVPVPKQLAHLPLPALKLPSPCKSKSLGDLTSEDIACNFESKYQCISKSFVTTGIRDKKGVTVKTKSLEPIDALTEQLRKLVSFDQEDNCQVLYSKQDANQLPRALVRKLSSRSQSRVRNIASRAKEKQEANKQKVPNPSNGAGVVLRNKPSAPTPAVNRHSTGSYIAGYLKNTKGGGLEGRGIPEGACTALHYGHVDQFCSDNSVLQTEPSSDDKPEIYFLLRL

Target ORF information:

RefSeq Version	XM_011512566.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C eta 1 (PLCH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512566.2

ORF Insert Sequence:

ATGAGTGTGA TGCAGTCCGG GACACAGATG ATCAAGCTGA AACGTGGAAC CAAAGGGCTT 
GTCCGCCTCT TTTACCTGGA TGAGCACCGG ACACGCCTCC GATGGCGACC CTCTAGGAAG 
AGTGAGAAGG CAAAAATACT TATTGATTCC ATTTACAAAG TGACTGAGGG CCGGCAGTCT 
GAAATATTCC ACAGACAAGC TGAGGGGAAC TTCGACCCCA GCTGCTGCTT CACCATCTAC 
CATGGCAACC ACATGGAGTC CCTGGACCTC ATCACCTCCA ACCCCGAGGA GGCCCGCACC 
TGGATCACAG GCCTCAAGTA CCTGATGGCT GGCATCAGTG ATGAAGACTC CCTTGCCAAA 
AGGCAGAGGA CCCATGACCA ATGGGTGAAG CAGACCTTTG AGGAAGCTGA TAAGAATGGT 
GACGGCTTGC TGAATATTGA AGAGATACAT CAGCTGATGC ATAAACTGAA TGTTAATCTG 
CCCCGAAGAA AAGTCAGACA AATGTTTCAG GAAGCCGACA CAGATGAGAA TCAGGGAACT 
TTGACATTTG AAGAGTTCTG TGTTTTTTAC AAAATGATGT CTTTGAGACG AGACCTTTAT 
TTGTTACTTT TGAGCTACAG TGACAAGAAA GATCACCTAA CTGTGGAAGA ACTGGCTCAG 
TTTTTGAAGG TGGAGCAAAA GATGAATAAT GTGACAACGG ACTATTGTCT TGACATCATA 
AAGAAGTTTG AAGTTTCAGA AGAAAATAAG GTGAAAAATG TTCTTGGCAT AGAAGGCTTC 
ACGAACTTCA TGCGTAGTCC TGCCTGTGAC ATATTTAACC CATTGCACCA TGAAGTGTAC 
CAAGACATGG ATCAGCCCCT CTGCAACTAC TACATTGCTT CCTCTCACAA TACATACCTG 
ACTGGAGACC AGCTCCTTTC TCAGTCCAAA GTGGATATGT ATGCACGGGT GCTGCAAGAG 
GGCTGTCGCT GTGTGGAAGT TGACTGTTGG GATGGCCCAG ATGGAGAGCC AGTAGTACAT 
CATGGTTACA CTCTCACTTC AAAAATTCTC TTCAGAGATG TTGTGGAGAC CATCAACAAG 
CATGCCTTTG TGAAGAATGA GTTTCCTGTT ATATTGTCTA TCGAGAATCA CTGCAGTATC 
CAGCAGCAAA GGAAGATTGC TCAGTACCTG AAAGGAATAT TCGGAGACAA ACTGGACCTG 
TCATCTGTTG ATACAGGGGA GTGCAAGCAG CTTCCAAGCC CTCAAAGTTT GAAAGGCAAA 
ATTCTAGTGA AGGGTAAGAA GTTGCCTTAT CACCTTGGGG ATGATGCAGA GGAAGGGGAA 
GTTTCTGATG AGGACAGTGC AGATGAAATT GAAGACGAGT GCAAATTCAA GCTCCATTAT 
AGTAATGGGA CCACTGAGCA TCAGGTGGAA TCTTTCATAA GGAAAAAACT GGAGTCACTG 
TTAAAAGAAT CTCAAATTCG AGATAAAGAA GATCCTGATA GTTTCACAGT GCGGGCACTA 
CTGAAGGCCA CGCATGAAGG CTTAAATGCA CACCTGAAGC AGAGTCCAGA TGTAAAGGAA 
AGTGGAAAGA AATCACATGG ACGATCCCTC ATGACCAACT TTGGAAAACA TAAGAAAACT 
ACAAAATCAC GGTCTAAATC TTACAGTACT GATGATGAGG AAGACACACA GCAGAGTACT 
GGCAAGGAGG GTGGCCAGCT GTACAGATTG GGTCGCCGAA GGAAAACCAT GAAGCTCTGC 
CGAGAACTCT CTGATTTGGT TGTGTACACA AACTCCGTGG CCGCTCAGGA CATTGTGGAT 
GACGGAACCA CAGGAAATGT GTTATCATTC AGTGAAACAA GAGCACATCA GGTTGTTCAG 
CAAAAATCAG AGCAGTTCAT GATTTATAAT CAAAAGCAAC TCACGAGGAT TTACCCCTCT 
GCCTACCGCA TTGATTCCAG TAACTTCAAC CCTCTCCCCT ACTGGAACGC AGGCTGCCAG 
CTAGTGGCAC TGAATTATCA ATCTGAAGGA CGAATGATGC AGTTAAACCG AGCCAAATTC 
AAGGCAAATG GCAATTGTGG CTATGTCCTC AAACCCCAGC AAATGTGCAA AGGTACTTTC 
AACCCTTTCT CTGGTGACCC TCTTCCTGCC AACCCCAAAA AGCAGCTCAT CCTGAAAGTT 
ATCAGTGGAC AGCAACTCCC CAAACCTCCA GACTCCATGT TTGGAGATCG AGGCGAGATC 
ATTGACCCTT TTGTTGAAGT TGAAATTATT GGATTGCCAG TAGATTGTTG TAAAGATCAA 
ACCCGTGTGG TAGATGACAA TGGATTTAAC CCTGTGTGGG AAGAAACACT GACATTTACA 
GTACACATGC CAGAAATAGC TTTGGTTCGG TTCCTTGTGT GGGATCACGA TCCCATTGGA 
CGAGACTTTG TTGGACAAAG AACTGTGACC TTCAGCAGCT TAGTGCCTGG CTACCGGCAT 
GTCTATTTGG AAGGACTGAC AGAAGCATCC ATATTTGTAC ACATAACCAT CAATGAAATC 
TATGGAAAGT GGAGCCCTTT AATACTCAAC CCCAGTTATA CTATATTGCA CTTTCTAGGA 
GCTACAAAGA ACAGACAACT CCAGGGTCTG AAGGGACTGT TCAATAAGAA TCCTAGGCAC 
AGTTCTTCAG AAAACAATTC CCATTATGTA CGGAAGCGAT CCATTGGAGA TAGAATTCTG 
CGACGCACAG CTAGCGCCCC AGCCAAAGGC AGGAAAAAGA GCAAAATGGG CTTCCAAGAA 
ATGGTGGAGA TAAAGGATTC TGTGTCCGAG GCCACAAGAG ATCAAGATGG CGTGCTGAGG 
AGGACCACAC GCAGTTTGCA AGCACGCCCT GTCTCTATGC CTGTTGACAG AAACCTTCTG 
GGAGCTTTGT CGCTGCCTGT ATCTGAAACA GCAAAAGACA TTGAAGGAAA AGAAAACTCT 
CTAGCAGAAG ATAAAGATGG CAGAAGAAAA GGGAAAGCAA GTATAAAAGA TCCACATTTT 
CTAAATTTCA ACAAAAAGTT ATCATCCTCC TCCAGTGCTC TGCTCCACAA AGATACCAGC 
CAAGGGGACA CCATTGTATC TACTGCCCAC ATGTCAGTCA CAGGAGAACA GCTGGGCATG 
TCAAGTCCTA GGGGTGGGAG AACCACATCA AATGCCACAA GCAATTGCCA GGAAAACCCC 
TGTCCCAGCA AGTCTCTCTC CCCAAAGCAG CATTTGGCTC CCGATCCTGT AGTTAACCCC 
ACACAAGATC TGCATGGTGT GAAAATCAAG GAAAAGGGTA ATCCTGAGGA CTTTGTGGAA 
GGGAAAAGCA TCTTGTCAGG AAGCGTCCTT TCTCATAGCA ACCTAGAAAT TAAGAACCTG 
GAAGGTAATA GGGGTAAGGG CCGAGCTGCA ACATCCTTTT CTTTGTCAGA CGTCTCCATG 
CTCTGTTCTG ACATACCTGA CCTACATTCA ACTGCAATTC TGCAGGAGAG TGTAATTTCC 
CATCTTATTG ACAATGTCAC TTTAACAAAT GAGAATGAGC CGGGCAGTTC CATCTCAGCC 
CTGATTGGCC AGTTTGATGA GACCAACAAT CAGGCTCTCA CAGTTGTTTC TCATCTTCAT 
AATACCAGTG TGATGTCAGG CCATTGTCCC TTGCCTAGCC TGGGCCTAAA AATGCCCATC 
AAGCATGGTT TTTGCAAGGG AAAATCCAAG TCTTCCTTCC TGTGCTCATC TCCGGAGCTG 
ATAGCACTCT CGAGTTCTGA GACCACCAAA CATGCAACGA ACACAGTTTA TGAAACTACC 
TGCACTCCCA TCTCTAAAAC CAAACCAGAT GATGACCTTT CTAGTAAGGC CAAGACAGCG 
GCCTTAGAAA GCAACCTGCC TGGATCCCCT AATACTTCTC GTGGCTGGTT ACCAAAAAGT 
CCTACCAAGG GAGAAGACTG GGAAACACTG AAGAGCTGCA GCCCTGCCTC TTCCCCTGAT 
TTGACCCTGG AGGATGTAAT AGCTGATCCC ACTCTCTGTT TCAATTCTGG GGAGAGCAGC 
CTTGTGGAAA TTGATGGAGA ATCAGAAAAT CTTTCTCTAA CAACCTGTGA ATATAGGAGA 
GAGGGCACAA GTCAACTTGC TTCTCCTTTA AAACTCAAGT ACAATCAGGG TGTGGTAGAA 
CACTTTCAAA GAGGTTTGAG AAACGGCTAC TGTAAAGAGA CCCTCCGCCC TTCTGTCCCT 
GAAATATTCA ACAATATTCA AGATGTCAAA ACTCAAAGTA TTTCTTATCT AGCCTATCAG 
GGTGCTGGCT TTGTGCATAA TCATTTCTCA GATTCAGATG CAAAAATGTT CCAGACCTGT 
GTGCCCCAGC AATCTAGTGC TCAAGATATG CATGTCCCTG TACCCAAGCA GTTGGCACAT 
CTTCCTTTGC CTGCTCTGAA ACTGCCTAGT CCTTGCAAAT CCAAAAGTCT GGGGGACTTA 
ACATCAGAGG ACATTGCCTG CAATTTTGAG AGCAAGTACC AGTGTATTAG TAAGAGTTTT 
GTTACAACTG GCATTAGAGA CAAGAAGGGC GTGACTGTGA AGACAAAGTC GTTAGAGCCT 
ATAGATGCCC TGACCGAGCA GCTTCGGAAG CTTGTGTCCT TTGACCAGGA AGACAACTGC 
CAAGTGCTAT ATTCAAAGCA GGATGCCAAT CAGCTCCCCC GGGCACTGGT CAGGAAGTTG 
TCATCCAGAA GTCAGAGCAG AGTGCGCAAT ATTGCTAGTC GTGCCAAGGA GAAACAGGAA 
GCCAACAAGC AGAAAGTTCC AAACCCCAGC AATGGGGCAG GAGTGGTTCT TAGAAACAAA 
CCCTCAGCAC CCACCCCTGC AGTGAATCGC CACTCCACCG GCTCCTACAT CGCAGGCTAC 
CTGAAGAACA CGAAAGGGGG TGGCCTTGAA GGCCGGGGCA TCCCAGAGGG GGCATGCACG 
GCTCTTCACT ATGGCCACGT TGACCAGTTT TGTTCAGATA ATTCTGTTTT GCAGACTGAG 
CCAAGCAGTG ATGATAAACC AGAAATTTAT TTTCTTTTGA GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression profile of PLC?, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation.
Andrology4(5)850-6(2016 Sep)
Tavalaee M,Nasr-Esfahani MH

A canonical EF-loop directs Ca(2+) -sensitivity in phospholipase C-?2.
Journal of cellular biochemistry115(3)557-65(2014 Mar)
Popovics P,Lu J,Nadia Kamil L,Morgan K,Millar RP,Schmid R,Blindauer CA,Stewart AJ

Association between polymorphisms in COMT, PLCH1, and CYP17A1, and non-small-cell lung cancer risk in Chinese nonsmokers.
Clinical lung cancer14(1)45-9(2013 Jan)
Zhang Y,Hua S,Zhang A,Kong X,Jiang C,Deng D,Wenlong B

Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
PLoS genetics9(1)e1003225(2013)
Lauc G,Huffman JE,Pu?i? M,Zgaga L,Adamczyk B,Mu?ini? A,Novokmet M,Pola?ek O,Gornik O,Kri?ti? J,Keser T,Vitart V,Scheijen B,Uh HW,Molokhia M,Patrick AL,McKeigue P,Kol?i? I,Luki? IK,Swann O,van Leeuwen FN,Ruhaak LR,Houwing-Duistermaat JJ,Slagboom PE,Beekman M,de Craen AJ,Deelder AM,Zeng Q,Wang W,Hastie ND,Gyllensten U,Wilson JF,Wuhrer M,Wright AF,Rudd PM,Hayward C,Aulchenko Y,Campbell H,Rudan I

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

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PLCH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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