Gja4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gja4
Entrez Gene ID	25655
Full Name	gap junction protein, alpha 4
Synonyms	CXN37
General protein information	Preferred Names gap junction protein, alpha 4 Names gap junction alpha-4 protein Gap junction membrane channel, protein alpha 4 (connexin 37) connexin-37 cx37 gap junction membrane channel protein alpha 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	gap junction protein that mediates cell-cell communication; may mediate hyperexcitability following nerve injury [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021654.2	NP_067686.1	gap junction alpha-4 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GJA4	NP_002051.2
Bos taurus (cattle)	GJA4	NP_001077207.1
Pan troglodytes (chimpanzee)	GJA4	XP_001166110.1
	LOC710913	XP_001108418.1
Canis lupus familiaris (dog)	GJA4	XP_005629029.1
Xenopus tropicalis (tropical clawed frog)	gja4	NP_001072904.1
Mus musculus (house mouse)	Gja4	NP_032146.1
Rattus norvegicus (Norway rat)	Gja4	NP_067686.1
Gallus gallus (chicken)	GJA4	NP_996867.3

Gene Ontology
Bibliography

Related articles in PubMed

Improving myocardial fractional flow reserve in coronary atherosclerosis via CX37 gene silence: a preclinical validation study in pigs.
Guo S, Yang Y, Yang Z, You H, Shi Y, Hu Z, Meng Z, Xiao J
Interactive cardiovascular and thoracic surgery26(1)139-145(2018 Jan)

Regulation of Cx37 channel and growth-suppressive properties by phosphorylation.
Jacobsen NL, Pontifex TK, Li H, Solan JL, Lampe PD, Sorgen PL, Burt JM
Journal of cell science130(19)3308-3321(2017 Oct)

Structural determinants and proliferative consequences of connexin 37 hemichannel function in insulinoma cells.
Good ME, Ek-Vitor?n JF, Burt JM
The Journal of biological chemistry289(44)30379-86(2014 Oct)

Impact of 7,12-dimethylbenz[a]anthracene exposure on connexin gap junction proteins in cultured rat ovaries.
Ganesan S, Keating AF
Toxicology and applied pharmacology274(2)209-14(2014 Jan)

Carboxy terminus and pore-forming domain properties specific to Cx37 are necessary for Cx37-mediated suppression of insulinoma cell proliferation.
Nelson TK, Sorgen PL, Burt JM
American journal of physiology. Cell physiology305(12)C1246-56(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cx37-N55I and -Q58L mutations formed hemichannels with behavior and permeation properties similar to Cx37-WT (especially Q58L), but none of the mutants suppressed rat insulinoma cell proliferation.
Title: Structural determinants and proliferative consequences of connexin 37 hemichannel function in insulinoma cells.

7,12-Dimethylbenz[a]anthracene increases Cx37 and Cx43 expression prior to follicle loss and during follicle loss both Cx37 and Cx43 expressions are reduced in cultured rat ovaries.
Title: Impact of 7,12-dimethylbenz[a]anthracene exposure on connexin gap junction proteins in cultured rat ovaries.

Differences in channel behavior of connexin 37 (Cx37) cytoplasmic loop mutants suggest that the Cx37-CT and pore-forming domains are both essential to growth suppression by Cx37.
Title: Carboxy terminus and pore-forming domain properties specific to Cx37 are necessary for Cx37-mediated suppression of insulinoma cell proliferation.

In the IVCs of IVC-banded ACF rats, which were exposed to a hemodynamic condition of high pressure and low flow velocity, the GJSNs and the TGJAs of Cx37 increased markedly.
Title: Differential endothelial gap junction expression in venous vessels exposed to different hemodynamics.

Renal epithelia express Cx37 in their basolateral membranes.
Title: Connexin 37 is localized in renal epithelia and responds to changes in dietary salt intake.

The following Gja4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gja4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12327	NM_021654.2 Latest version!	Rattus norvegicus gap junction protein, alpha 4 (Gja4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gja4 ( NM_021654.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067686.1 Rattus norvegicus gap junction protein, alpha 4 (Gja4), mRNA.

Price & Availability↑

CloneID	ORa12327
Clone ID Related Accession (Same CDS sequence)	NM_021654.2
Accession Version	NM_021654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	gap junction alpha-4 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078837.1. On Aug 8, 2004 this sequence version replaced NM_021654.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078837.1, EX490393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGACT GGGGCTTCCT GGAGAAGCTG CTAGACCAGG TCCAGGAGCA CTCGACCGTG 
GTGGGCAAGA TCTGGTTAAC GGTGCTCTTC ATCTTCCGAA TCCTCATCCT GGGCCTGGCT 
GGTGAGTCGG TGTGGGGCGA CGAGCAGTCT GATTTCGAAT GTAACACAGC CCAGCCAGGC 
TGCACCAATG TGTGCTATGA CCAGGCTTTC CCCATCTCCC ACATCCGATA CTGGGTGCTG 
CAGTTCCTCT TCGTGAGCAC ACCCACCCTG ATCTACCTGG GCCACGTCAT TTACCTGTCT 
CGGCGGGAAG AACGGTTGCG GCAGAAAGAG GGAGAGCTTC GGGCACTGCC ATCCAAGGAC 
CCTCATGTAG AGCGGGCACT AGCAGCCATA GAACATCAGA TGGCCAAGAT CTCGGTGGCA 
GAGGACGGTC GTCTTCGCAT TCGAGGGGCG CTCATGGGTA CCTACGTGAT CAGTGTGCTG 
TGTAAGAGTG TGCTGGAGGC AGGCTTCCTC TATGGCCAGT GGCGTCTCTA CGGCTGGACC 
ATGGAGCCGG TGTTTGTGTG CCAGCGTGCG CCCTGCCCCC ACGTCGTGGA CTGCTACGTC 
TCTCGACCCA CCGAGAAGAC CATCTTCATC ATTTTCATGT TGGTGGTAGG GGTCATCTCC 
TTGGTGCTCA ACCTACTGGA GCTGGTGCAC CTGTTGTGCC GCTGTGTCAG CCGGGAGATA 
AAGGCACGGA GGGACCATGA CACCCGCCCG GCTCAGGGCA GTGCCTCAGA CCCTTACCCT 
GAACAGGTTT TCTTCTACCT CCCCATGGGC GAGGGACCCT CGTCCCCACC GTGTCCCACC 
TACAATGGGC TCTCATCCAC TGAGCAAAAC TGGGCCAATT TGACCACCGA GGAGAGACTG 
ACCTCTACCA GACCTCCCCC ATTTGTAAAT GCAGCCCCCC AGGGTGGCCA GAAGTCCTCT 
AGCCGCCCCA ACAGCTCTGC ATCCAAGAAG CAGTATGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067686.1
CDS	78..1079
Translation	MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPGCTNVCYDQAFPISHIRYWVLQFLFVSTPTLIYLGHVIYLSRREERLRQKEGELRALPSKDPHVERALAAIEHQMAKISVAEDGRLRIRGALMGTYVISVLCKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPHVVDCYVSRPTEKTIFIIFMLVVGVISLVLNLLELVHLLCRCVSREIKARRDHDTRPAQGSASDPYPEQVFFYLPMGEGPSSPPCPTYNGLSSTEQNWANLTTEERLTSTRPPPFVNAAPQGGQKSSSRPNSSASKKQYV

Target ORF information:

RefSeq Version	NM_021654.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, alpha 4 (Gja4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021654.2

ORF Insert Sequence:

ATGGGTGACT GGGGCTTCCT GGAGAAGCTG CTAGACCAGG TCCAGGAGCA CTCGACCGTG 
GTGGGCAAGA TCTGGTTAAC GGTGCTCTTC ATCTTCCGAA TCCTCATCCT GGGCCTGGCT 
GGTGAGTCGG TGTGGGGCGA CGAGCAGTCT GATTTCGAAT GTAACACAGC CCAGCCAGGC 
TGCACCAATG TGTGCTATGA CCAGGCTTTC CCCATCTCCC ACATCCGATA CTGGGTGCTG 
CAGTTCCTCT TCGTGAGCAC ACCCACCCTG ATCTACCTGG GCCACGTCAT TTACCTGTCT 
CGGCGGGAAG AACGGTTGCG GCAGAAAGAG GGAGAGCTTC GGGCACTGCC ATCCAAGGAC 
CCTCATGTAG AGCGGGCACT AGCAGCCATA GAACATCAGA TGGCCAAGAT CTCGGTGGCA 
GAGGACGGTC GTCTTCGCAT TCGAGGGGCG CTCATGGGTA CCTACGTGAT CAGTGTGCTG 
TGTAAGAGTG TGCTGGAGGC AGGCTTCCTC TATGGCCAGT GGCGTCTCTA CGGCTGGACC 
ATGGAGCCGG TGTTTGTGTG CCAGCGTGCG CCCTGCCCCC ACGTCGTGGA CTGCTACGTC 
TCTCGACCCA CCGAGAAGAC CATCTTCATC ATTTTCATGT TGGTGGTAGG GGTCATCTCC 
TTGGTGCTCA ACCTACTGGA GCTGGTGCAC CTGTTGTGCC GCTGTGTCAG CCGGGAGATA 
AAGGCACGGA GGGACCATGA CACCCGCCCG GCTCAGGGCA GTGCCTCAGA CCCTTACCCT 
GAACAGGTTT TCTTCTACCT CCCCATGGGC GAGGGACCCT CGTCCCCACC GTGTCCCACC 
TACAATGGGC TCTCATCCAC TGAGCAAAAC TGGGCCAATT TGACCACCGA GGAGAGACTG 
ACCTCTACCA GACCTCCCCC ATTTGTAAAT GCAGCCCCCC AGGGTGGCCA GAAGTCCTCT 
AGCCGCCCCA ACAGCTCTGC ATCCAAGAAG CAGTATGTAT AG