Neto cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Neto
Entrez Gene ID	32303
Full Name	Neuropilin and tolloid-like
Synonyms	CG12727,CG15751,CG15752,CG15753,CG32635,CG44328,CT36003,Dmel\CG44328,Dmel_CG15753,Dmel_CG32635,NETO,net,neto,tld-like
General protein information	Preferred Names Neuropilin and tolloid-like Names CG44328 gene product from transcript CG44328-RB CG44328-PA CG44328-PB Neto-PA Neto-PB neuropilin and tolloid-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12A2-12A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298282.1	NP_001285211.1	neuropilin and tolloid-like, isoform B
NM_167371.2	NP_727708.1	neuropilin and tolloid-like, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

only Neto-beta restored PAK synaptic accumulation at neto null NMJs. Thus, Neto engages in intracellular interactions that regulate the iGluR subtype composition by preferentially recruiting and/or stabilizing selective receptor subtypes.
Title: Neto-mediated intracellular interactions shape postsynaptic composition at the Drosophila neuromuscular junction.

neto null mutants rescued with a Neto variant that cannot be processed have severely impaired NMJs and reduced iGluRs synaptic clusters. Unprocessed Neto retains the ability to engage iGluRs in vivo and to form complexes with normal synaptic transmission
Title: Prodomain removal enables neto to stabilize glutamate receptors at the Drosophila neuromuscular junction.

these studies reveal that Drosophila Neto is a novel, essential component of the iGluR complexes and is required for iGluR clustering, organization of PSDs, and synapse functionality.
Title: Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction.

The following Neto gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Neto cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01503	NM_167371.2 Latest version!	Drosophila melanogaster neuropilin and tolloid-like, transcript variant A (Neto), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa21460	NM_001298282.1 Latest version!	Drosophila melanogaster neuropilin and tolloid-like, transcript variant B (Neto), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Neto ( NM_167371.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727708.1 Drosophila melanogaster neuropilin and tolloid-like, transcript variant A (Neto), mRNA.

Price & Availability↑

CloneID	OFa01503
Clone ID Related Accession (Same CDS sequence)	NM_167371.2
Accession Version	NM_167371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuropilin and tolloid-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167371.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAAGAA GAGGAAGCAC TTGCAGCGCA CAACAACCAC AGCAGCGGCC ACACAAACAG 
CAGCAGCAGC AGCAGCAGCA AAATCAAAGG TTAATCCCCG GCCAACGCTG TTCGCTGCTA 
ATGTTGCTAA TTGTTGGCCA AGCAGTTTAC GCTTCTGCCG GCAGTGAGAT AATCGCAAAT 
CGTTCGGCTT CAGCTGGCGA ATCGTTGATA ATGCTGTCGA ATTTCACCGT TGCCACGCCG 
CCTCCCGCCC CAAGATTCAC CAGTCCCTTT CGGGAGCAGG ATGTGGCTGA GGGGGAGGAG 
TTGGCGGGTG GAGGGCAGGT CGATTGGTTG GACGAGCAGC ATCCGCTGAC CATTTCCCGA 
CCGCCAAGAG CAGGACGCAG CGAGCGGCAG GCAGAAGAGG ATCAGGTGGA CCGCTGTCGT 
CTCTTTGTCG AGGGGGATCC CACCAAGAAT GAGCTGTACA GCCCCGAGTA TCCCAACTTG 
TATCCGAAGA ACATCAACTG CACCCGGGTG ATAACAGCGC CAAAGGGACA AATCATACGC 
CTGGACTTTC GCAACTCGTT TAATATCGAG GCCAAGGAGG GGTGCAAATT TGATTTTCTC 
GAAATACGAG ATGGCCAGTA CGGTTTCTCT ACGCTGATTG GCAAGTTCTG CGGCACCGAC 
TTTCCGCCGG AGATCACCTC GAAGGAGCGG TATTTGTGGC TTCATTTCCA CTCCGACGAG 
ACCATCGAGT ACACCGGATT CAGTGCGGTC TATGAGTATC TGGACAGGAG TCGGGATGCG 
CCCAGCACCG ATCTCAACTG CACCATCGAT AAGGGCGGCT TCGAGGGCTT CATCAACTCC 
ACGGATGTTC CGGCCGAGAT CTGGGAGCAG GTCAATCGCA ATAAGATCGC GCTGGACTGC 
ATCTGGCGCA TTCAGGTCAA GGAGAATTGG AAGATATTTC TCAAGTTTCT GGACTTCAAG 
CTGAGCAAGC CGAACGATTG TCAAACCAAC TTCCTGGATA TATTCCCGGA GCAGACGGTG 
ATGCCACTGA GGGTTAAGAA CTTTTGCGGT TCAGCTGGCG AGAGTATTAC GGCGGAGTCA 
AATATCCTGC ACTTGCGCTT CTACGCAGAT CAAACCGCGA TTAACTCGAC ATTTGGCATT 
CTGTTCACGG CGTTCCGGGA ACGTGGAGCT GCCTGCACCG AGGACGAGTA CGATTGCGAG 
GACGCGACCT GCATATCAAA GGATTTGAAA TGTAATAACC TAGACAATTG TAAATTTCGA 
TGGGACGAGG AGGGCTGCAC GAGCGAGGCG GCCGGCCAAT CGGAGCACGT GGTCATCATC 
GTGATTGTGT TTGGCCTGAT ACTCGGCGGA ATGGTCATTA CGTTCATCGT CAATTGCATA 
CGCAAGATAA TACGTGATCA GAAGATAATA CGCGACGTGC CCGCGTTGAG TGACGGAATC 
TATCACATTG ATTCCTTTAT ACTGGAAGCG CCCAAGAGCG CGATTTTCGT GGGATTACAG 
AATGATTTCA TGTGCGATTT CTCCTACTCA TCGGACGGGC AAATGGCGCC CAACTTGGGG 
CATGAGACCC GAGATGAGGA GGCCGCAGTT GGTGGCGATT CCGACTTTGA TTCGCAGATG 
GACTCGCCGA AGCAGACGTG TGACTGTGAT TTCGAGTTGG ATGTGAACGA TGGCGAACAA 
ATGGAGGAAG ACGATGAAGA GGAGCTGGAG GAAGAGCAGG AGCAGGACCA TCATGTGGAG 
GATCAGGAGT ACGACGATGG CATTGGTTTC AGTTGTGACA GCGACAGCGG ACTCACACTG 
CCCGAGGACG ATGATGAGTT TGTACTGGTC ACCGGACAGT GTCTGCCCAG CAACTCCAGT 
CACTCGTGCA GCAGTTCGAT GCAATGCAGC AGCGGTTCTA GTGGCAGAGA GGTGGCTACT 
AGCTCCTCAA ACTCCATGGA ACTGGACATG GTTCTAACAC CGCCACCGCC ACCAACACTG 
GGCAAGCCCA AGTCCGGTGT ACATCCCCTC AAGTTCCTGC ACAAAATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727708.1
CDS	597..2630
Translation	MRRRGSTCSAQQPQQRPHKQQQQQQQQNQRLIPGQRCSLLMLLIVGQAVYASAGSEIIANRSASAGESLIMLSNFTVATPPPAPRFTSPFREQDVAEGEELAGGGQVDWLDEQHPLTISRPPRAGRSERQAEEDQVDRCRLFVEGDPTKNELYSPEYPNLYPKNINCTRVITAPKGQIIRLDFRNSFNIEAKEGCKFDFLEIRDGQYGFSTLIGKFCGTDFPPEITSKERYLWLHFHSDETIEYTGFSAVYEYLDRSRDAPSTDLNCTIDKGGFEGFINSTDVPAEIWEQVNRNKIALDCIWRIQVKENWKIFLKFLDFKLSKPNDCQTNFLDIFPEQTVMPLRVKNFCGSAGESITAESNILHLRFYADQTAINSTFGILFTAFRERGAACTEDEYDCEDATCISKDLKCNNLDNCKFRWDEEGCTSEAAGQSEHVVIIVIVFGLILGGMVITFIVNCIRKIIRDQKIIRDVPALSDGIYHIDSFILEAPKSAIFVGLQNDFMCDFSYSSDGQMAPNLGHETRDEEAAVGGDSDFDSQMDSPKQTCDCDFELDVNDGEQMEEDDEEELEEEQEQDHHVEDQEYDDGIGFSCDSDSGLTLPEDDDEFVLVTGQCLPSNSSHSCSSSMQCSSGSSGREVATSSSNSMELDMVLTPPPPPTLGKPKSGVHPLKFLHKII

Target ORF information:

RefSeq Version	NM_167371.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuropilin and tolloid-like, transcript variant A (Neto), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167371.2

ORF Insert Sequence:

ATGCGAAGAA GAGGAAGCAC TTGCAGCGCA CAACAACCAC AGCAGCGGCC ACACAAACAG 
CAGCAGCAGC AGCAGCAGCA AAATCAAAGG TTAATCCCCG GCCAACGCTG TTCGCTGCTA 
ATGTTGCTAA TTGTTGGCCA AGCAGTTTAC GCTTCTGCCG GCAGTGAGAT AATCGCAAAT 
CGTTCGGCTT CAGCTGGCGA ATCGTTGATA ATGCTGTCGA ATTTCACCGT TGCCACGCCG 
CCTCCCGCCC CAAGATTCAC CAGTCCCTTT CGGGAGCAGG ATGTGGCTGA GGGGGAGGAG 
TTGGCGGGTG GAGGGCAGGT CGATTGGTTG GACGAGCAGC ATCCGCTGAC CATTTCCCGA 
CCGCCAAGAG CAGGACGCAG CGAGCGGCAG GCAGAAGAGG ATCAGGTGGA CCGCTGTCGT 
CTCTTTGTCG AGGGGGATCC CACCAAGAAT GAGCTGTACA GCCCCGAGTA TCCCAACTTG 
TATCCGAAGA ACATCAACTG CACCCGGGTG ATAACAGCGC CAAAGGGACA AATCATACGC 
CTGGACTTTC GCAACTCGTT TAATATCGAG GCCAAGGAGG GGTGCAAATT TGATTTTCTC 
GAAATACGAG ATGGCCAGTA CGGTTTCTCT ACGCTGATTG GCAAGTTCTG CGGCACCGAC 
TTTCCGCCGG AGATCACCTC GAAGGAGCGG TATTTGTGGC TTCATTTCCA CTCCGACGAG 
ACCATCGAGT ACACCGGATT CAGTGCGGTC TATGAGTATC TGGACAGGAG TCGGGATGCG 
CCCAGCACCG ATCTCAACTG CACCATCGAT AAGGGCGGCT TCGAGGGCTT CATCAACTCC 
ACGGATGTTC CGGCCGAGAT CTGGGAGCAG GTCAATCGCA ATAAGATCGC GCTGGACTGC 
ATCTGGCGCA TTCAGGTCAA GGAGAATTGG AAGATATTTC TCAAGTTTCT GGACTTCAAG 
CTGAGCAAGC CGAACGATTG TCAAACCAAC TTCCTGGATA TATTCCCGGA GCAGACGGTG 
ATGCCACTGA GGGTTAAGAA CTTTTGCGGT TCAGCTGGCG AGAGTATTAC GGCGGAGTCA 
AATATCCTGC ACTTGCGCTT CTACGCAGAT CAAACCGCGA TTAACTCGAC ATTTGGCATT 
CTGTTCACGG CGTTCCGGGA ACGTGGAGCT GCCTGCACCG AGGACGAGTA CGATTGCGAG 
GACGCGACCT GCATATCAAA GGATTTGAAA TGTAATAACC TAGACAATTG TAAATTTCGA 
TGGGACGAGG AGGGCTGCAC GAGCGAGGCG GCCGGCCAAT CGGAGCACGT GGTCATCATC 
GTGATTGTGT TTGGCCTGAT ACTCGGCGGA ATGGTCATTA CGTTCATCGT CAATTGCATA 
CGCAAGATAA TACGTGATCA GAAGATAATA CGCGACGTGC CCGCGTTGAG TGACGGAATC 
TATCACATTG ATTCCTTTAT ACTGGAAGCG CCCAAGAGCG CGATTTTCGT GGGATTACAG 
AATGATTTCA TGTGCGATTT CTCCTACTCA TCGGACGGGC AAATGGCGCC CAACTTGGGG 
CATGAGACCC GAGATGAGGA GGCCGCAGTT GGTGGCGATT CCGACTTTGA TTCGCAGATG 
GACTCGCCGA AGCAGACGTG TGACTGTGAT TTCGAGTTGG ATGTGAACGA TGGCGAACAA 
ATGGAGGAAG ACGATGAAGA GGAGCTGGAG GAAGAGCAGG AGCAGGACCA TCATGTGGAG 
GATCAGGAGT ACGACGATGG CATTGGTTTC AGTTGTGACA GCGACAGCGG ACTCACACTG 
CCCGAGGACG ATGATGAGTT TGTACTGGTC ACCGGACAGT GTCTGCCCAG CAACTCCAGT 
CACTCGTGCA GCAGTTCGAT GCAATGCAGC AGCGGTTCTA GTGGCAGAGA GGTGGCTACT 
AGCTCCTCAA ACTCCATGGA ACTGGACATG GTTCTAACAC CGCCACCGCC ACCAACACTG 
GGCAAGCCCA AGTCCGGTGT ACATCCCCTC AAGTTCCTGC ACAAAATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neto ( NM_001298282.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285211.1 Drosophila melanogaster neuropilin and tolloid-like, transcript variant B (Neto), mRNA.

Price & Availability↑

CloneID	OFa21460
Clone ID Related Accession (Same CDS sequence)	NM_001298282.1
Accession Version	NM_001298282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuropilin and tolloid-like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAAGAA GAGGAAGCAC TTGCAGCGCA CAACAACCAC AGCAGCGGCC ACACAAACAG 
CAGCAGCAGC AGCAGCAGCA AAATCAAAGG TTAATCCCCG GCCAACGCTG TTCGCTGCTA 
ATGTTGCTAA TTGTTGGCCA AGCAGTTTAC GCTTCTGCCG GCAGTGAGAT AATCGCAAAT 
CGTTCGGCTT CAGCTGGCGA ATCGTTGATA ATGCTGTCGA ATTTCACCGT TGCCACGCCG 
CCTCCCGCCC CAAGATTCAC CAGTCCCTTT CGGGAGCAGG ATGTGGCTGA GGGGGAGGAG 
TTGGCGGGTG GAGGGCAGGT CGATTGGTTG GACGAGCAGC ATCCGCTGAC CATTTCCCGA 
CCGCCAAGAG CAGGACGCAG CGAGCGGCAG GCAGAAGAGG ATCAGGTGGA CCGCTGTCGT 
CTCTTTGTCG AGGGGGATCC CACCAAGAAT GAGCTGTACA GCCCCGAGTA TCCCAACTTG 
TATCCGAAGA ACATCAACTG CACCCGGGTG ATAACAGCGC CAAAGGGACA AATCATACGC 
CTGGACTTTC GCAACTCGTT TAATATCGAG GCCAAGGAGG GGTGCAAATT TGATTTTCTC 
GAAATACGAG ATGGCCAGTA CGGTTTCTCT ACGCTGATTG GCAAGTTCTG CGGCACCGAC 
TTTCCGCCGG AGATCACCTC GAAGGAGCGG TATTTGTGGC TTCATTTCCA CTCCGACGAG 
ACCATCGAGT ACACCGGATT CAGTGCGGTC TATGAGTATC TGGACAGGAG TCGGGATGCG 
CCCAGCACCG ATCTCAACTG CACCATCGAT AAGGGCGGCT TCGAGGGCTT CATCAACTCC 
ACGGATGTTC CGGCCGAGAT CTGGGAGCAG GTCAATCGCA ATAAGATCGC GCTGGACTGC 
ATCTGGCGCA TTCAGGTCAA GGAGAATTGG AAGATATTTC TCAAGTTTCT GGACTTCAAG 
CTGAGCAAGC CGAACGATTG TCAAACCAAC TTCCTGGATA TATTCCCGGA GCAGACGGTG 
ATGCCACTGA GGGTTAAGAA CTTTTGCGGT TCAGCTGGCG AGAGTATTAC GGCGGAGTCA 
AATATCCTGC ACTTGCGCTT CTACGCAGAT CAAACCGCGA TTAACTCGAC ATTTGGCATT 
CTGTTCACGG CGTTCCGGGA ACGTGGAGCT GCCTGCACCG AGGACGAGTA CGATTGCGAG 
GACGCGACCT GCATATCAAA GGATTTGAAA TGTAATAACC TAGACAATTG TAAATTTCGA 
TGGGACGAGG AGGGCTGCAC GAGCGAGGCG GCCGGCCAAT CGGAGCACGT GGTCATCATC 
GTGATTGTGT TTGGCCTGAT ACTCGGCGGA ATGGTCATTA CGTTCATCGT CAATTGCATA 
CGCAAGATAA TACGTGATCA GAAGATAATA CGCGAGCACA TCCGAGAGTC GAAGGAGAGC 
AAGCTGGACG AGATGGGCCG CAACTCCAAG GGACGCTCGC GGGAGAATAT ATCCAGGCAA 
AAGCACTCGC AAACATCGCT GCAGATCCTG GATGATGTAT CCAATCGATA CTATCGCGAG 
GCGGTTCCAC TATCGTCGCA GTCCAGCAAG GCGGACTTCA AGGAGAAGGA GCACAGCATT 
CTGCGCCGCC ATGCCGATAT GACGCAAACA AGTCTCTGCG GCGTTGGCGA GGATGATGGC 
GAATCCTCAT CGACGACGAC GGCCACACAC GAGATGATGA CTAAGGCGGC CATGTCGGCG 
GTGCCCTCCA ATGCATGCGA CATGGGCTGT CAGACGAGGG AATCGCTGTT TGTCAACAGT 
CCCGGAATTG GACCGTCGGC GGGTCCTGGT GTTGGTGTTG CTGTTGGCAT CGGTATCGCC 
GTCGCTCCCG GCGGCGGTGT TGCCACGCTA ATGAGACAAA AGTCCAGCTC CTCATCGCTG 
GGCGTTGGTG GTGGCGCTGG GGGCGGGGCC ATGATGATGC CGCCCCCGCC ACCGCGATTC 
TTCTCCACCT TTGGCTATGA GCCATCGGGA TCGCCGGCGG TGGCAACGCT GACAAGGCGC 
AGCATGCACC AGCAGCAGCA ACATCCGCAG ATGCCGCGCC AAGAGTCCAT GGAGATGGAG 
GAGCGACACA GCGGCCGCTC GCACTACGGC GGCCTGCTGG TGACCTCGAC GGCCAAGCAG 
CCGCAGGAGA TCTGTCACCA TCATCACCAG CATCAGCAGC AGCATCAGCA GCAGCATCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAG CAACAGCAGC AGCGCATGCA CCATGCACAT 
CCGCATCCGC CGAGTATTGC TGCACGTTTG CCTGCGATGA AGGGGCATGG AACAGTCATG 
GGACAGACTA CGATCCTGCC CGGCGGCAAC AAGCAGCAGC AGCAACATCA GCAAAAGAAC 
GAGGAGTCCA AAGTGTTTAT CGACATACGG AATTCAGCGC CAGACGTGAT TATCATGACG 
TCCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285211.1
CDS	597..3065
Translation	MRRRGSTCSAQQPQQRPHKQQQQQQQQNQRLIPGQRCSLLMLLIVGQAVYASAGSEIIANRSASAGESLIMLSNFTVATPPPAPRFTSPFREQDVAEGEELAGGGQVDWLDEQHPLTISRPPRAGRSERQAEEDQVDRCRLFVEGDPTKNELYSPEYPNLYPKNINCTRVITAPKGQIIRLDFRNSFNIEAKEGCKFDFLEIRDGQYGFSTLIGKFCGTDFPPEITSKERYLWLHFHSDETIEYTGFSAVYEYLDRSRDAPSTDLNCTIDKGGFEGFINSTDVPAEIWEQVNRNKIALDCIWRIQVKENWKIFLKFLDFKLSKPNDCQTNFLDIFPEQTVMPLRVKNFCGSAGESITAESNILHLRFYADQTAINSTFGILFTAFRERGAACTEDEYDCEDATCISKDLKCNNLDNCKFRWDEEGCTSEAAGQSEHVVIIVIVFGLILGGMVITFIVNCIRKIIRDQKIIREHIRESKESKLDEMGRNSKGRSRENISRQKHSQTSLQILDDVSNRYYREAVPLSSQSSKADFKEKEHSILRRHADMTQTSLCGVGEDDGESSSTTTATHEMMTKAAMSAVPSNACDMGCQTRESLFVNSPGIGPSAGPGVGVAVGIGIAVAPGGGVATLMRQKSSSSSLGVGGGAGGGAMMMPPPPPRFFSTFGYEPSGSPAVATLTRRSMHQQQQHPQMPRQESMEMEERHSGRSHYGGLLVTSTAKQPQEICHHHHQHQQQHQQQHQQQQQQQQQQQQQQQRMHHAHPHPPSIAARLPAMKGHGTVMGQTTILPGGNKQQQQHQQKNEESKVFIDIRNSAPDVIIMTSH

Target ORF information:

RefSeq Version	NM_001298282.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuropilin and tolloid-like, transcript variant B (Neto), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298282.1

ORF Insert Sequence:

ATGCGAAGAA GAGGAAGCAC TTGCAGCGCA CAACAACCAC AGCAGCGGCC ACACAAACAG 
CAGCAGCAGC AGCAGCAGCA AAATCAAAGG TTAATCCCCG GCCAACGCTG TTCGCTGCTA 
ATGTTGCTAA TTGTTGGCCA AGCAGTTTAC GCTTCTGCCG GCAGTGAGAT AATCGCAAAT 
CGTTCGGCTT CAGCTGGCGA ATCGTTGATA ATGCTGTCGA ATTTCACCGT TGCCACGCCG 
CCTCCCGCCC CAAGATTCAC CAGTCCCTTT CGGGAGCAGG ATGTGGCTGA GGGGGAGGAG 
TTGGCGGGTG GAGGGCAGGT CGATTGGTTG GACGAGCAGC ATCCGCTGAC CATTTCCCGA 
CCGCCAAGAG CAGGACGCAG CGAGCGGCAG GCAGAAGAGG ATCAGGTGGA CCGCTGTCGT 
CTCTTTGTCG AGGGGGATCC CACCAAGAAT GAGCTGTACA GCCCCGAGTA TCCCAACTTG 
TATCCGAAGA ACATCAACTG CACCCGGGTG ATAACAGCGC CAAAGGGACA AATCATACGC 
CTGGACTTTC GCAACTCGTT TAATATCGAG GCCAAGGAGG GGTGCAAATT TGATTTTCTC 
GAAATACGAG ATGGCCAGTA CGGTTTCTCT ACGCTGATTG GCAAGTTCTG CGGCACCGAC 
TTTCCGCCGG AGATCACCTC GAAGGAGCGG TATTTGTGGC TTCATTTCCA CTCCGACGAG 
ACCATCGAGT ACACCGGATT CAGTGCGGTC TATGAGTATC TGGACAGGAG TCGGGATGCG 
CCCAGCACCG ATCTCAACTG CACCATCGAT AAGGGCGGCT TCGAGGGCTT CATCAACTCC 
ACGGATGTTC CGGCCGAGAT CTGGGAGCAG GTCAATCGCA ATAAGATCGC GCTGGACTGC 
ATCTGGCGCA TTCAGGTCAA GGAGAATTGG AAGATATTTC TCAAGTTTCT GGACTTCAAG 
CTGAGCAAGC CGAACGATTG TCAAACCAAC TTCCTGGATA TATTCCCGGA GCAGACGGTG 
ATGCCACTGA GGGTTAAGAA CTTTTGCGGT TCAGCTGGCG AGAGTATTAC GGCGGAGTCA 
AATATCCTGC ACTTGCGCTT CTACGCAGAT CAAACCGCGA TTAACTCGAC ATTTGGCATT 
CTGTTCACGG CGTTCCGGGA ACGTGGAGCT GCCTGCACCG AGGACGAGTA CGATTGCGAG 
GACGCGACCT GCATATCAAA GGATTTGAAA TGTAATAACC TAGACAATTG TAAATTTCGA 
TGGGACGAGG AGGGCTGCAC GAGCGAGGCG GCCGGCCAAT CGGAGCACGT GGTCATCATC 
GTGATTGTGT TTGGCCTGAT ACTCGGCGGA ATGGTCATTA CGTTCATCGT CAATTGCATA 
CGCAAGATAA TACGTGATCA GAAGATAATA CGCGAGCACA TCCGAGAGTC GAAGGAGAGC 
AAGCTGGACG AGATGGGCCG CAACTCCAAG GGACGCTCGC GGGAGAATAT ATCCAGGCAA 
AAGCACTCGC AAACATCGCT GCAGATCCTG GATGATGTAT CCAATCGATA CTATCGCGAG 
GCGGTTCCAC TATCGTCGCA GTCCAGCAAG GCGGACTTCA AGGAGAAGGA GCACAGCATT 
CTGCGCCGCC ATGCCGATAT GACGCAAACA AGTCTCTGCG GCGTTGGCGA GGATGATGGC 
GAATCCTCAT CGACGACGAC GGCCACACAC GAGATGATGA CTAAGGCGGC CATGTCGGCG 
GTGCCCTCCA ATGCATGCGA CATGGGCTGT CAGACGAGGG AATCGCTGTT TGTCAACAGT 
CCCGGAATTG GACCGTCGGC GGGTCCTGGT GTTGGTGTTG CTGTTGGCAT CGGTATCGCC 
GTCGCTCCCG GCGGCGGTGT TGCCACGCTA ATGAGACAAA AGTCCAGCTC CTCATCGCTG 
GGCGTTGGTG GTGGCGCTGG GGGCGGGGCC ATGATGATGC CGCCCCCGCC ACCGCGATTC 
TTCTCCACCT TTGGCTATGA GCCATCGGGA TCGCCGGCGG TGGCAACGCT GACAAGGCGC 
AGCATGCACC AGCAGCAGCA ACATCCGCAG ATGCCGCGCC AAGAGTCCAT GGAGATGGAG 
GAGCGACACA GCGGCCGCTC GCACTACGGC GGCCTGCTGG TGACCTCGAC GGCCAAGCAG 
CCGCAGGAGA TCTGTCACCA TCATCACCAG CATCAGCAGC AGCATCAGCA GCAGCATCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAACAG CAACAGCAGC AGCGCATGCA CCATGCACAT 
CCGCATCCGC CGAGTATTGC TGCACGTTTG CCTGCGATGA AGGGGCATGG AACAGTCATG 
GGACAGACTA CGATCCTGCC CGGCGGCAAC AAGCAGCAGC AGCAACATCA GCAAAAGAAC 
GAGGAGTCCA AAGTGTTTAT CGACATACGG AATTCAGCGC CAGACGTGAT TATCATGACG 
TCCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
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Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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