ERAS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERAS
Entrez Gene ID	3266
Full Name	ES cell expressed Ras
Synonyms	HRAS2,HRASP
General protein information	Preferred Names ES cell expressed Ras Names GTPase ERas E-Ras embryonic stem cell-expressed Ras small GTPase protein E-Ras v-Ha-ras Harvey rat sarcoma viral oncogene homolog 2 v-Ha-ras Harvey rat sarcoma viral oncogene homolog pseudogene
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.23
Summary	This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012].
Disorder MIM:	300437

mRNA and Protein(s)

mRNA	Protein	Name
NM_181532.3	NP_853510.1	GTPase ERas precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ERAS	XP_002700122.2
Mus musculus (house mouse)	Eras	NP_853526.1
Homo sapiens (human)	ERAS	NP_853510.1
Rattus norvegicus (Norway rat)	Eras	NP_001102845.1
Rattus norvegicus (Norway rat)	LOC100911243	XP_003752059.2
	ERAS	XP_001101805.1
Canis lupus familiaris (dog)	ERAS	XP_548988.1

Gene Ontology
Bibliography

Related articles in PubMed

Insertional mutagenesis in a HER2-positive breast cancer model reveals ERAS as a driver of cancer and therapy resistance.
Ikink GJ, Boer M, Bakker ERM, Vendel-Zwaagstra A, Klijn C, Ten Hoeve J, Jonkers J, Wessels LF, Hilkens J
Oncogene(2018 Jan)

The Function of Embryonic Stem Cell-expressed RAS (E-RAS), a Unique RAS Family Member, Correlates with Its Additional Motifs and Its Structural Properties.
Nakhaei-Rad S, Nakhaeizadeh H, Kordes C, Cirstea IC, Schmick M, Dvorsky R, Bastiaens PI, H?ussinger D, Ahmadian MR
The Journal of biological chemistry290(25)15892-903(2015 Jun)

BRAF inhibitor resistance mediated by the AKT pathway in an oncogenic BRAF mouse melanoma model.
Perna D, Karreth FA, Rust AG, Perez-Mancera PA, Rashid M, Iorio F, Alifrangis C, Arends MJ, Bosenberg MW, Bollag G, Tuveson DA, Adams DJ
Proceedings of the National Academy of Sciences of the United States of America112(6)E536-45(2015 Feb)

Role of the ERas gene in gastric cancer cells.
Liu Y, Wang Z, Li H, Wu Z, Wei F, Wang H
Oncology reports30(1)50-6(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

N-terminal extension of E-RAS is important for E-RAS signaling activity.
Title: The Function of Embryonic Stem Cell-expressed RAS (E-RAS), a Unique RAS Family Member, Correlates with Its Additional Motifs and Its Structural Properties.

Expression of ERAS in human melanoma cell lines conferred resistance to PLX4720 and induced hyperphosphorylation of AKT.
Title: BRAF inhibitor resistance mediated by the AKT pathway in an oncogenic BRAF mouse melanoma model.

After knocking down the ERas gene by siRNA, we observed that there was a significant decrease in proliferation, metastasis as well as clonality in gastric carcinoma.
Title: Role of the ERas gene in gastric cancer cells.

Data indicate that expression of ERAS, LHX1, and CCRK is increased in aggressive subgroups of medulloblastomas.
Title: Functional genomics identifies drivers of medulloblastoma dissemination.

ERas may be a potential biomarker for gastric cancer, but advanced studies are wanted.
Title: Detection of ?-catenin, gastrokine-2 and embryonic stem cell expressed ras in gastric cancers.

The following ERAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu07581	NM_181532.3 Latest version!	Homo sapiens ES cell expressed Ras (ERAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERAS ( NM_181532.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_853510.1 Homo sapiens ES cell expressed Ras (ERAS), mRNA.

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CloneID	OHu07581
Clone ID Related Accession (Same CDS sequence)	NM_181532.3
Accession Version	NM_181532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	GTPase ERas precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY043532.1 and BC101642.1. On Dec 5, 2012 this sequence version replaced NM_181532.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY043532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000636362.1/ ENSP00000490793.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAGCTGC CAACAAAGCC TGGCACCTTC GACCTGGGCC TGGCCACATG GAGCCCTTCC 
TTCCAGGGGG AAACCCACCG GGCTCAGGCA CGCCGCAGGG ATGTTGGCAG GCAGCTGCCT 
GAGTACAAGG CTGTGGTGGT GGGCGCCAGT GGCGTGGGCA AGAGTGCGCT GACCATCCAG 
CTGAACCACC AGTGCTTCGT GGAGGACCAC GACCCCACCA TCCAGGATTC CTACTGGAAG 
GAGTTGACCC TGGACAGTGG GGACTGCATT CTGAATGTGC TGGACACAGC AGGGCAGGCC 
ATCCATAGGG CCCTGCGTGA CCAGTGCCTG GCTGTCTGTG ATGGTGTGCT GGGCGTCTTC 
GCTCTCGATG ACCCCTCGTC TCTGATCCAG CTGCAGCAGA TATGGGCCAC CTGGGGCCCT 
CACCCCGCCC AGCCCCTTGT CCTCGTGGGC AACAAGTGTG ACCTTGTGAC CACTGCTGGA 
GATGCTCATG CCGCTGCTGC AGCCCTCGCA CACAGCTGGG GGGCCCACTT CGTGGAGACC 
TCGGCCAAAA CACGGCAAGG CGTGGAGGAG GCCTTTTCCC TGCTGGTCCA TGAGATCCAG 
AGGGTCCAGG AGGCCATGGC GAAGGAGCCC ATGGCAAGGT CCTGTAGGGA GAAGACCCGG 
CACCAGAAGG CCACCTGCCA CTGTGGCTGC TCTGTGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853510.1
CDS	81..782
Translation	MELPTKPGTFDLGLATWSPSFQGETHRAQARRRDVGRQLPEYKAVVVGASGVGKSALTIQLNHQCFVEDHDPTIQDSYWKELTLDSGDCILNVLDTAGQAIHRALRDQCLAVCDGVLGVFALDDPSSLIQLQQIWATWGPHPAQPLVLVGNKCDLVTTAGDAHAAAAALAHSWGAHFVETSAKTRQGVEEAFSLLVHEIQRVQEAMAKEPMARSCREKTRHQKATCHCGCSVA

Target ORF information:

RefSeq Version	NM_181532.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ES cell expressed Ras (ERAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181532.3

ORF Insert Sequence:

ATGGAGCTGC CAACAAAGCC TGGCACCTTC GACCTGGGCC TGGCCACATG GAGCCCTTCC 
TTCCAGGGGG AAACCCACCG GGCTCAGGCA CGCCGCAGGG ATGTTGGCAG GCAGCTGCCT 
GAGTACAAGG CTGTGGTGGT GGGCGCCAGT GGCGTGGGCA AGAGTGCGCT GACCATCCAG 
CTGAACCACC AGTGCTTCGT GGAGGACCAC GACCCCACCA TCCAGGATTC CTACTGGAAG 
GAGTTGACCC TGGACAGTGG GGACTGCATT CTGAATGTGC TGGACACAGC AGGGCAGGCC 
ATCCATAGGG CCCTGCGTGA CCAGTGCCTG GCTGTCTGTG ATGGTGTGCT GGGCGTCTTC 
GCTCTCGATG ACCCCTCGTC TCTGATCCAG CTGCAGCAGA TATGGGCCAC CTGGGGCCCT 
CACCCCGCCC AGCCCCTTGT CCTCGTGGGC AACAAGTGTG ACCTTGTGAC CACTGCTGGA 
GATGCTCATG CCGCTGCTGC AGCCCTCGCA CACAGCTGGG GGGCCCACTT CGTGGAGACC 
TCGGCCAAAA CACGGCAAGG CGTGGAGGAG GCCTTTTCCC TGCTGGTCCA TGAGATCCAG 
AGGGTCCAGG AGGCCATGGC GAAGGAGCCC ATGGCAAGGT CCTGTAGGGA GAAGACCCGG 
CACCAGAAGG CCACCTGCCA CTGTGGCTGC TCTGTGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insertional mutagenesis in a HER2-positive breast cancer model reveals ERAS as a driver of cancer and therapy resistance.
Oncogene(2018 Jan)
Ikink GJ,Boer M,Bakker ERM,Vendel-Zwaagstra A,Klijn C,Ten Hoeve J,Jonkers J,Wessels LF,Hilkens J

The Function of Embryonic Stem Cell-expressed RAS (E-RAS), a Unique RAS Family Member, Correlates with Its Additional Motifs and Its Structural Properties.
The Journal of biological chemistry290(25)15892-903(2015 Jun)
Nakhaei-Rad S,Nakhaeizadeh H,Kordes C,Cirstea IC,Schmick M,Dvorsky R,Bastiaens PI,H?ussinger D,Ahmadian MR

BRAF inhibitor resistance mediated by the AKT pathway in an oncogenic BRAF mouse melanoma model.
Proceedings of the National Academy of Sciences of the United States of America112(6)E536-45(2015 Feb)
Perna D,Karreth FA,Rust AG,Perez-Mancera PA,Rashid M,Iorio F,Alifrangis C,Arends MJ,Bosenberg MW,Bollag G,Tuveson DA,Adams DJ

Role of the ERas gene in gastric cancer cells.
Oncology reports30(1)50-6(2013 Jul)
Liu Y,Wang Z,Li H,Wu Z,Wei F,Wang H

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ERAS cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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