DEK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEK1
Entrez Gene ID	841981
Full Name	calpain-type cysteine protease family
Synonyms	ATDEK1,DEFECTIVE KERNEL 1,EMB1275,EMB80,EMBRYO DEFECTIVE 80,F7A10.23,F7A10_23,embryo defective 1275
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_180635.1	NP_850966.1	calpain-type cysteine protease family
NM_104411.4	NP_175932.2	calpain-type cysteine protease family
NM_001333707.1	NP_001321273.1	calpain-type cysteine protease family
NM_180636.1	NP_850967.1	calpain-type cysteine protease family
NM_001333706.1	NP_001319240.1	calpain-type cysteine protease family
NM_001198309.1	NP_001185238.1	calpain-type cysteine protease family

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0709400	NP_001047890.1
Arabidopsis thaliana (thale cress)	DEK1	NP_001185238.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel role for DEK1 in the regulation of leaf epidermal cell wall structure, is reported.
Title: DEFECTIVE KERNEL1 (DEK1) Regulates Cell Walls in the Leaf Epidermis.

A role for DEK1 in regulating microtubule-orchestrated cell wall orientation during cell division can explain its effects on embryonic development, and suggests a more general function for calpains in microtubule organization in eukaryotic cells.
Title: Calpain-Mediated Positional Information Directs Cell Wall Orientation to Sustain Plant Stem Cell Activity, Growth and Development.

DEK1 influences HD-ZIP IV gene expression, and thus epidermis differentiation, by promoting cell adhesion and communication in the epidermis.
Title: DEFECTIVE KERNEL 1 promotes and maintains plant epidermal differentiation.

DEK1 has a role in growth regulation.
Title: The phytocalpain defective kernel 1 is a novel Arabidopsis growth regulator whose activity is regulated by proteolytic processing.

Data suggest a model for aleurone cell fate specification in which DEK1 perceives and/or transmits a positional signal
Title: Subcellular localization and functional domain studies of DEFECTIVE KERNEL1 in maize and Arabidopsis suggest a model for aleurone cell fate specification involving CRINKLY4 and SUPERNUMERARY ALEURONE LAYER1.

The following DEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb26461	NM_001198309.1 Latest version!	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb30462	NM_001333707.1 Latest version!	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb30462	NM_180635.1 Latest version!	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb30462	NM_104411.4 Latest version!	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb30462	NM_001333706.1 Latest version!	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb30462	NM_180636.1 Latest version!	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEK1 ( NM_001198309.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185238.1 Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Price & Availability↑

CloneID	OAb26461
Clone ID Related Accession (Same CDS sequence)	NM_001198309.1
Accession Version	NM_001198309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	calpain-type cysteine protease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG GTTAAAACTT ATTTTTATAT TCTGCAAGTG 
TTCTTTCTTA TGATGCTGAT TAATGGAGAA TTAACTAAGA AAAGTTATTA CCAGACTGGA 
ATATCTGGGG CAGTTTGTGT TCTTGATGAT GAACCAATGA TAAGTGGTAA ACATTGCGGC 
CAAATGGACT CAAGTGTCTG TCAAAGTCAG AAGATTAGCT TTTCCGTTAC AGCAATGATC 
CAATCCGATT CTGGACCTGT ATGTCTTTTT GGCACTGAAT TTCAAAAGAA AGTATGTTGG 
GAGATTCTGG TTGCTGGTTC TGAGCAAGGA ATTGAGGCTG GCCAAGTTGG GCTTAGGTTG 
ATAACAAAAG GTGAGAGGCA GACAACCGTT GCTAGAGAGT GGTATATTGG TGCAACCAGC 
ATAACTGATG GAAGGTGGCA TACAGTGACA ATCACAATTG ATGCTGATGC GGGGGAAGCT 
ACTTGTTACA TAGATGGTGG GTTTGATGGC TACCAGAATG GGTTACCTCT AAGTATTGGC 
AGTGCCATTT GGGAACAAGG AGCTGAAGTT TGGTTGGGTG TTAGGCCACC TATAGATGTT 
GATGCATTCG GGAGATCAGA TAGTGATGGC GTCGAATCAA AGATGCATAT TATGGATGTT 
TTCCTTTGGG GGAAATGCTT AAGTGAAGAA GAGGCCGCTT CTTTGCATGC AGCCATTGGC 
ATGGCTGACT TAGACATGAT TGATTTGTCT GATGACAATT GGCAATGGAC GGATTCACCC 
CCCAGAGTCG ATGGTTGGGA TAGTGATCCT GCCGATGTTG ATCTCTATGA TAGGGATGAC 
GTAGATTGGG ATGGACAATA TTCCAGTGGG AGGAAAAGAA GATCAGGTCG GGATTTTGTA 
ATGAGTGTCG ATTCCTTTGC CAGGAGACAC AGGAAACCCA GGATGGAGAC ACAAGAAGAT 
ATAAATCAAA GAATGCGTTC AGTTGAGTTG GCTGTCAAAG AAGCTCTCTC TGCACGAGGT 
GATAAGCAAT TTACTGACCA GGAATTTCCT CCAAATGATC GCTCTTTATT TGTGGATACA 
CAAAATCCCC CATCAAAATT GCAGGTTGTT TCTGAATGGA TGAGACCTGA CTCCATTGTG 
AAAGAAAACG GTAGTGATTC CCGTCCCTGC CTGTTCTCTG GGGATGCAAA TCCTTCAGAT 
GTTTGCCAGG GGCGTTTGGG GGATTGTTGG TTCTTAAGCG CCGTTGCAGT TTTGACAGAG 
GTTTCACGAA TATCTGAAGT GATCATTACT CCTGAATACA ACGAGGAAGG GATCTACACT 
GTTCGTTTTT GTATTCAGGG TGAGTGGGTT CCTGTTGTTA TCGATGACTG GATTCCATGT 
GAATCACCTG GTAAACCAGC TTTTGCTACT AGCAGAAAGC TCAATGAACT CTGGGTCTCC 
ATGGTGGAGA AAGCATATGC CAAGCTCCAT GGTTCTTATG AGGCACTGGA GGGGGGACTG 
GTTCAGGATG CTCTTGTCGA CCTAACTGGA GGAGCTGGTG AGGAGATTGA CTTGCGGAGT 
GCTCAAGCAC AAATAGATCT TGCAAGTGGC AGATTGTGGT CTCAATTGTT ACGTTTTAAA 
CAAGAGGGGT TCTTACTTGG TGCTGGAAGT CCATCAGGAT CTGATGTTCA TGTATCTTCC 
AGTGGCATTG TGCAAGGGCA TGCTTACTCC GTCTTACAGG TGAGAGAGGT TGATGGGCAC 
AGACTTGTTC AGATTCGAAA TCCATGGGCT AATGAAGTTG AGTGGAATGG TCCCTGGTCA 
GACTCATCCC CAGAGTGGAC TGATAGGATG AAGCACAAGC TGAAGCATGT TCCACAGTCA 
AAAGAAGGTA TATTCTGGAT GTCTTGGCAA GATTTCCAGA TTCATTTCAG ATCAATATAT 
GTGTGTCGGG TTTACCCCCG TGAGATGCGC TACTCTGTAA ATGGCCAATG GCGAGGTTAT 
AGTGCCGGTG GCTGCCAAGA TTATAGCTCA TGGCATCAAA ATCCACAATT CAGGCTGAGG 
GCAACTGGTT CTGATGCATC TTTACCAATT CATGTGTTCA TCACCTTAAC TCAGGGCGTA 
GGTTTCTCGA GAACAACTCC TGGATTTCGT AACTACCAAT CAAGCCATGA TTCACAGTTG 
TTCTATATCG GATTGAGGAT TCTTAAAACT CGTGGACGTC GTGCTGCTTA CAACATATTT 
CTTCATGAAT CTGTTGGTGG AACAGACTAT GTGAATTCCC GTGAGATTTC ATGTGAAATG 
GTTCTTGACC CTGATCCTAA GGGTTATACT ATTGTCCCAA CCACGATACA CCCAGGGGAA 
GAAGCACCTT TTGTCCTTTC AGTCTTCACA AAAGCATCCA TTGTTCTTGA AGCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185238.1
CDS	433..6972
Translation	MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQVKTYFYILQVFFLMMLINGELTKKSYYQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL

Target ORF information:

RefSeq Version	NM_001198309.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198309.1

ORF Insert Sequence:

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG GTTAAAACTT ATTTTTATAT TCTGCAAGTG 
TTCTTTCTTA TGATGCTGAT TAATGGAGAA TTAACTAAGA AAAGTTATTA CCAGACTGGA 
ATATCTGGGG CAGTTTGTGT TCTTGATGAT GAACCAATGA TAAGTGGTAA ACATTGCGGC 
CAAATGGACT CAAGTGTCTG TCAAAGTCAG AAGATTAGCT TTTCCGTTAC AGCAATGATC 
CAATCCGATT CTGGACCTGT ATGTCTTTTT GGCACTGAAT TTCAAAAGAA AGTATGTTGG 
GAGATTCTGG TTGCTGGTTC TGAGCAAGGA ATTGAGGCTG GCCAAGTTGG GCTTAGGTTG 
ATAACAAAAG GTGAGAGGCA GACAACCGTT GCTAGAGAGT GGTATATTGG TGCAACCAGC 
ATAACTGATG GAAGGTGGCA TACAGTGACA ATCACAATTG ATGCTGATGC GGGGGAAGCT 
ACTTGTTACA TAGATGGTGG GTTTGATGGC TACCAGAATG GGTTACCTCT AAGTATTGGC 
AGTGCCATTT GGGAACAAGG AGCTGAAGTT TGGTTGGGTG TTAGGCCACC TATAGATGTT 
GATGCATTCG GGAGATCAGA TAGTGATGGC GTCGAATCAA AGATGCATAT TATGGATGTT 
TTCCTTTGGG GGAAATGCTT AAGTGAAGAA GAGGCCGCTT CTTTGCATGC AGCCATTGGC 
ATGGCTGACT TAGACATGAT TGATTTGTCT GATGACAATT GGCAATGGAC GGATTCACCC 
CCCAGAGTCG ATGGTTGGGA TAGTGATCCT GCCGATGTTG ATCTCTATGA TAGGGATGAC 
GTAGATTGGG ATGGACAATA TTCCAGTGGG AGGAAAAGAA GATCAGGTCG GGATTTTGTA 
ATGAGTGTCG ATTCCTTTGC CAGGAGACAC AGGAAACCCA GGATGGAGAC ACAAGAAGAT 
ATAAATCAAA GAATGCGTTC AGTTGAGTTG GCTGTCAAAG AAGCTCTCTC TGCACGAGGT 
GATAAGCAAT TTACTGACCA GGAATTTCCT CCAAATGATC GCTCTTTATT TGTGGATACA 
CAAAATCCCC CATCAAAATT GCAGGTTGTT TCTGAATGGA TGAGACCTGA CTCCATTGTG 
AAAGAAAACG GTAGTGATTC CCGTCCCTGC CTGTTCTCTG GGGATGCAAA TCCTTCAGAT 
GTTTGCCAGG GGCGTTTGGG GGATTGTTGG TTCTTAAGCG CCGTTGCAGT TTTGACAGAG 
GTTTCACGAA TATCTGAAGT GATCATTACT CCTGAATACA ACGAGGAAGG GATCTACACT 
GTTCGTTTTT GTATTCAGGG TGAGTGGGTT CCTGTTGTTA TCGATGACTG GATTCCATGT 
GAATCACCTG GTAAACCAGC TTTTGCTACT AGCAGAAAGC TCAATGAACT CTGGGTCTCC 
ATGGTGGAGA AAGCATATGC CAAGCTCCAT GGTTCTTATG AGGCACTGGA GGGGGGACTG 
GTTCAGGATG CTCTTGTCGA CCTAACTGGA GGAGCTGGTG AGGAGATTGA CTTGCGGAGT 
GCTCAAGCAC AAATAGATCT TGCAAGTGGC AGATTGTGGT CTCAATTGTT ACGTTTTAAA 
CAAGAGGGGT TCTTACTTGG TGCTGGAAGT CCATCAGGAT CTGATGTTCA TGTATCTTCC 
AGTGGCATTG TGCAAGGGCA TGCTTACTCC GTCTTACAGG TGAGAGAGGT TGATGGGCAC 
AGACTTGTTC AGATTCGAAA TCCATGGGCT AATGAAGTTG AGTGGAATGG TCCCTGGTCA 
GACTCATCCC CAGAGTGGAC TGATAGGATG AAGCACAAGC TGAAGCATGT TCCACAGTCA 
AAAGAAGGTA TATTCTGGAT GTCTTGGCAA GATTTCCAGA TTCATTTCAG ATCAATATAT 
GTGTGTCGGG TTTACCCCCG TGAGATGCGC TACTCTGTAA ATGGCCAATG GCGAGGTTAT 
AGTGCCGGTG GCTGCCAAGA TTATAGCTCA TGGCATCAAA ATCCACAATT CAGGCTGAGG 
GCAACTGGTT CTGATGCATC TTTACCAATT CATGTGTTCA TCACCTTAAC TCAGGGCGTA 
GGTTTCTCGA GAACAACTCC TGGATTTCGT AACTACCAAT CAAGCCATGA TTCACAGTTG 
TTCTATATCG GATTGAGGAT TCTTAAAACT CGTGGACGTC GTGCTGCTTA CAACATATTT 
CTTCATGAAT CTGTTGGTGG AACAGACTAT GTGAATTCCC GTGAGATTTC ATGTGAAATG 
GTTCTTGACC CTGATCCTAA GGGTTATACT ATTGTCCCAA CCACGATACA CCCAGGGGAA 
GAAGCACCTT TTGTCCTTTC AGTCTTCACA AAAGCATCCA TTGTTCTTGA AGCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEK1 ( NM_180635.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850966.1 Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

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CloneID	OAb30462
Clone ID Related Accession (Same CDS sequence)	NM_180635.1 , NM_104411.4 , NM_001333707.1 , NM_180636.1 , NM_001333706.1
Accession Version	NM_180635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	calpain-type cysteine protease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850966.1
CDS	122..6577
Translation	MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL

Target ORF information:

RefSeq Version	NM_180635.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180635.1

ORF Insert Sequence:

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEK1 ( NM_104411.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175932.2 Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

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CloneID	OAb30462
Clone ID Related Accession (Same CDS sequence)	NM_180635.1 , NM_104411.4 , NM_001333707.1 , NM_180636.1 , NM_001333706.1
Accession Version	NM_104411.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	calpain-type cysteine protease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_104411.3.

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175932.2
CDS	445..6900
Translation	MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL

Target ORF information:

RefSeq Version	NM_104411.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_104411.4

ORF Insert Sequence:

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEK1 ( NM_001333707.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321273.1 Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

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CloneID	OAb30462
Clone ID Related Accession (Same CDS sequence)	NM_180635.1 , NM_104411.4 , NM_001333707.1 , NM_180636.1 , NM_001333706.1
Accession Version	NM_001333707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	calpain-type cysteine protease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321273.1
CDS	445..6900
Translation	MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL

Target ORF information:

RefSeq Version	NM_001333707.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333707.1

ORF Insert Sequence:

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEK1 ( NM_180636.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850967.1 Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

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CloneID	OAb30462
Clone ID Related Accession (Same CDS sequence)	NM_180635.1 , NM_104411.4 , NM_001333707.1 , NM_180636.1 , NM_001333706.1
Accession Version	NM_180636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	calpain-type cysteine protease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850967.1
CDS	122..6577
Translation	MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL

Target ORF information:

RefSeq Version	NM_180636.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180636.1

ORF Insert Sequence:

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEK1 ( NM_001333706.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319240.1 Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

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CloneID	OAb30462
Clone ID Related Accession (Same CDS sequence)	NM_180635.1 , NM_104411.4 , NM_001333707.1 , NM_180636.1 , NM_001333706.1
Accession Version	NM_001333706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	calpain-type cysteine protease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319240.1
CDS	448..6903
Translation	MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL

Target ORF information:

RefSeq Version	NM_001333706.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana calpain-type cysteine protease family (DEK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333706.1

ORF Insert Sequence:

ATGGAAGGGG ATGAGCGAGG AGTCTTACTT GCTTGTGTAA TTTCGGGTAC CCTTTTCACG 
GTTTTCGGTT CGGGTTCGTT TTGGATACTT TGGGCTGTTA ATTGGCGGCC ATGGCGTCTC 
TACAGTTGGA TCTTTGCTAG AAAATGGCCA AAAGTATTGC AAGGTCCTCA GCTTGATATA 
CTATGTGGTG TTCTATCTCT TTTTGCTTGG ATTGTGGTAG TATCCCCTAT TGCAATCTTG 
ATAGGATGGG GTTCTTGGCT GATTGTGATA TTGGATCGAC ATATCATTGG GCTGGCGATA 
ATAATGGCTG GAACAGCCCT TTTACTGGCA TTCTACTCAA TCATGCTTTG GTGGAGGACC 
CAGTGGCAAA GCTCAAGAGC TGTCGCTTTA CTTCTCCTTC TTGGTGTTGC CTTACTATGT 
GCGTATGAAC TCTGTGCTGT CTATGTTACG GCTGGTGCGC ATGCATCTCA GCAATATTCT 
CCTTCTGGTT TCTTTTTCGG TGTATCAGCA ATCGCGTTGG CAATTAACAT GCTATTTATC 
TGCCGCATGG TCTTTAATGG AAATGGTTTA GATGTGGACG AATATGTAAG GAGGGCATAT 
AAATTTGCTT ATTCAGATTG TATAGAAGTG GGTCCTGTGG CTTGTTTGCC TGAACCTCCT 
GATCCTAATG AATTATATCC CCGGCAAACC AGCAGGGCTT CACATCTTGG CCTTCTGTAC 
CTGGGCTCAC TCGTAGTTCT CCTTGCCTAC TCAGTCCTAT ATGGTCTCAC AGCTAGGGAA 
TCACGTTGGC TTGGAGGAAT CACATCAGCT GCAGTTATTG TTCTTGACTG GAATATTGGG 
GCATGCTTGT ATGGGTTTAA GCTTCTTCAG AATCGTGTTC TGGCACTTTT TGTTGCTGGC 
ATATCCCGTC TTTTCCTAAT ATGTTTTGGC ATACACTACT GGTACCTAGG GCATTGTATT 
AGTTACATTT TCGTAGCATC AGTTCTATCA GGTGCTGCTG TTTCTCGGCA TCTATCTATA 
ACAGACCCAT CAGCTGCAAG AAGAGATGCC TTACAGAGCA CAGTGATCCG CTTGAGAGAA 
GGTTTTCGGA GAAAAGAGCA GAATAGTTCT TCAAGTTCTT CAGATGGTTG TGGCTCAAGT 
ATAAAAAGAA GTAGTAGTAT CGATGCTGGC CATACTGGTT GTACTAATGA AGCAAATCGT 
ACGGCAGAAT CTTGCACGGC TGACAATCTA ACTCGAACAG GCAGCTCTCA GGAGGGAATC 
AATAGCGACA AAAGCGAAGA AAGTGGAAGA CCAAGCTTAG GTTTACGTAG TAGTTCATGT 
CGTTCTGTGG TCCAAGAGCC CGAAGCAGGA ACGTCTTATT TTATGGACAA AGTTTCTGAT 
CAAAATAACA CTCTTGTTGT TTGTTCGAGC AGTGGTCTAG ATAGCCAAGG TTACGAGTCT 
AGCACATCGA ATTCTGCAAA CCAGCAGCTT TTGGATATGA ATTTGGCTCT TGCTTTCCAG 
GACCAGTTAA ACAATCCTAG GATAGCCTCG ATACTTAAGA AGAAAGCAAA AGAAGGTGAT 
CTTGAACTGA CTAATTTGCT GCAAGACAAG GGGTTGGACC CTAACTTTGC TGTAATGTTG 
AAGGAAAAAA ACTTGGATCC TACTATATTG GCACTACTTC AGAGGAGTAG TTTGGATGCA 
GATAGAGATC ACCGCGACAA TACTGATATT ACAATCATTG ACTCAAACAG TGTTGACAAT 
ACTTTGCCAA ATCAGATTTC TTTATCCGAA GAATTGAGAC TCCGTGGACT AGAGAAGTGG 
CTTAAGTTGT CTAGACTTCT TCTGCACCAT GTAGCGGGGA CACCAGAGAG AGCATGGGGC 
CTCTTTAGTC TTGTCTTTAT CCTTGAAACA ATCATTGTGG CCATTTTTCG CCCAAAGACC 
ATCACGATTA TAAATTCTAG TCATCAACAG TTCGAATTTG GTTTCTCTGT GCTGCTATTG 
TCACCTGTTG TCTGTTCAAT AATGGCTTTT CTTCGGTCTC TTCAAGTTGA GGAAATGGCC 
TTGACATCAA AATCTCGCAA GTATGGCTTT GTTGCCTGGC TTCTGAGCAC ATCAGTTGGA 
TTGTCACTCT CGTTCTTGAG TAAATCGTCA GTACTTCTGG GAATATCCTT GACTGTGCCC 
CTCATGGCAG CATGCCTGTC TATTGCTGTT CCCATATGGA TGCATAATGG GTACCAATTT 
TGGGTTCCAC AGTTATCATG TGGTGACCAG GCAAGAGATT TACGATCTCC CAGGATAAAG 
GGGTTTATTC TTTGGATTTG TGTTGTGTTG TTTGCGGGTT CTGTAATTTC TCTTGGTGCG 
ATTATATCTG CTAAACCTTT GGATGATTTA AAGTATAAGC TGTTTAGTGC CAGAGAAAAC 
AACGTCACGT CACCATATAC ATCTTCTGTA TACCTTGGTT GGGCAATGTC ATCTGGAATT 
GCTTTAGTAG TTACCGCCAT TCTACCAATA GTTTCATGGT TTGCAACTTA TAGGTTTTCC 
CACTCTTCTG CTGTCTGTCT CATGATATTC TCAGTTGTTC TCGTGGCATT TTGTGGAACT 
TCATATTTGG AAGTTGTAAA ATCTAGAGAT GATCAGTTGC CCACAAAGGG TGATTTCCTT 
GCGGCCTTGC TTCCACTTGC ATGCATTCCG GCGCTGCTTT CACTATGCTG TGGGATGGTT 
AAATGGAAGG ACGATTGTTG GATACTCTCT CGAGGTGTAT ATGTTTTCTT TTCAATAGGT 
CTTCTTCTTC TTTTTGGTGC GATAGCAGCT GTCATTGCAG TCAAACCATG GACGATAGGC 
GTATCTTTTC TCTTAGTTCT TTTCCTTATG GTGGTAACAA TTGGTGTAAT CCATCTTTGG 
GCGTCAAACA ATTTCTATTT AACCAGGAAA CAGACATCCT TTGTCTGCTT TCTTGCTCTT 
CTTTTGGGTT TGGCCGCATT CCTTCTCGGA TGGCATCAAG ATAAAGCATT TGCTGGAGCA 
TCTGTTGGTT ACTTTACATT CCTGTCTCTG TTGGCTGGAA GAGCATTAGC TGTTCTTCTA 
TCCCCACCAA TTGTAGTATA TTCTCCAAGG GTGCTACCAG TATATGTCTA CGATGCTCAT 
GCTGATTGCG GAAAGAATGT CAGTGCTGCA TTTCTTGTCC TGTATGGAAT TGCTTTGGCA 
ACAGAAGGCT GGGGTGTTGT TGCTAGTCTG ATAATTTATC CTCCGTTTGC GGGTGCTGCT 
GTATCAGCTA TCACCCTTGT AGTAGCCTTT GGGTTTGCTG TTTCTCGCCC ATGTTTGACT 
CTTGAGATGA TGGAGGTTGC TGTACGCTTT CTTAGCAAGG ATACTATAGT GCAAGCTATC 
TCTCGATCTG CCACGAAAAC AAGAAATGCT CTATCCGGCA CGTATTCAGC TCCCCAAAGG 
TCCGCCAGCT CTGCAGCTCT TCTGGTTGGG GATCCCTCTG CAATGCGTGA TAAAGCAGGG 
AACTTTGTGC TTCCTAGAGA TGATGTCATG AAATTAAGGG ATCGTCTCAG GAACGAAGAA 
AGAGTTGCTG GATCAATCTT CTACAAAATG CAATGCAGGA AAGGATTCCG TCATGAACCA 
CCTACAAATG TAGATTATAG AAGAGACATG TGTGCCCATG CAAGAGTTTT GGCACTGGAA 
GAGGCAATTG ATACAGAATG GGTGTATATG TGGGACAAAT TTGGTGGTTA TTTACTACTA 
TTGTTAGGTT TGACAGCTAA GGCGGAGAGA GTTCAGGATG AGGTACGGTT GCGGCTCTTC 
TTAGATAGCA TTGGGTTCTC GGATTTAAGT GCCAGAAAAA TCAGTAAATG GAAGCCAGAG 
GATAGAAGAC AATTCGAAAT TATTCAAGAG AGTTATCTGA GAGAGAAAGA GATGGAAGAG 
GAAAGCCTTA TGCAGAGACG TGAAGAAGAA GGGAGAGGTA AAGAAAGAAG GAAAGCTCTT 
TTGGAGAAGG AAGAGCGCAA ATGGAAGGAA ATTGAAGCGT CCCTTATTCC ATCTATTCCT 
AATGCTGGTA GCAGGGAGGC AGCAGCCATG GCAGCTGCAA TACGTGCTGT TGGGGGTGAT 
TCTGTCCTTG AGGATTCCTT CGCAAGAGAG AGGGTCTCGG GTATTGCACG TAGGATACGC 
ACTGCTCAAC TAGAACGACG TGCACAACAG ACTGGAATAT CTGGGGCAGT TTGTGTTCTT 
GATGATGAAC CAATGATAAG TGGTAAACAT TGCGGCCAAA TGGACTCAAG TGTCTGTCAA 
AGTCAGAAGA TTAGCTTTTC CGTTACAGCA ATGATCCAAT CCGATTCTGG ACCTGTATGT 
CTTTTTGGCA CTGAATTTCA AAAGAAAGTA TGTTGGGAGA TTCTGGTTGC TGGTTCTGAG 
CAAGGAATTG AGGCTGGCCA AGTTGGGCTT AGGTTGATAA CAAAAGGTGA GAGGCAGACA 
ACCGTTGCTA GAGAGTGGTA TATTGGTGCA ACCAGCATAA CTGATGGAAG GTGGCATACA 
GTGACAATCA CAATTGATGC TGATGCGGGG GAAGCTACTT GTTACATAGA TGGTGGGTTT 
GATGGCTACC AGAATGGGTT ACCTCTAAGT ATTGGCAGTG CCATTTGGGA ACAAGGAGCT 
GAAGTTTGGT TGGGTGTTAG GCCACCTATA GATGTTGATG CATTCGGGAG ATCAGATAGT 
GATGGCGTCG AATCAAAGAT GCATATTATG GATGTTTTCC TTTGGGGGAA ATGCTTAAGT 
GAAGAAGAGG CCGCTTCTTT GCATGCAGCC ATTGGCATGG CTGACTTAGA CATGATTGAT 
TTGTCTGATG ACAATTGGCA ATGGACGGAT TCACCCCCCA GAGTCGATGG TTGGGATAGT 
GATCCTGCCG ATGTTGATCT CTATGATAGG GATGACGTAG ATTGGGATGG ACAATATTCC 
AGTGGGAGGA AAAGAAGATC AGGTCGGGAT TTTGTAATGA GTGTCGATTC CTTTGCCAGG 
AGACACAGGA AACCCAGGAT GGAGACACAA GAAGATATAA ATCAAAGAAT GCGTTCAGTT 
GAGTTGGCTG TCAAAGAAGC TCTCTCTGCA CGAGGTGATA AGCAATTTAC TGACCAGGAA 
TTTCCTCCAA ATGATCGCTC TTTATTTGTG GATACACAAA ATCCCCCATC AAAATTGCAG 
GTTGTTTCTG AATGGATGAG ACCTGACTCC ATTGTGAAAG AAAACGGTAG TGATTCCCGT 
CCCTGCCTGT TCTCTGGGGA TGCAAATCCT TCAGATGTTT GCCAGGGGCG TTTGGGGGAT 
TGTTGGTTCT TAAGCGCCGT TGCAGTTTTG ACAGAGGTTT CACGAATATC TGAAGTGATC 
ATTACTCCTG AATACAACGA GGAAGGGATC TACACTGTTC GTTTTTGTAT TCAGGGTGAG 
TGGGTTCCTG TTGTTATCGA TGACTGGATT CCATGTGAAT CACCTGGTAA ACCAGCTTTT 
GCTACTAGCA GAAAGCTCAA TGAACTCTGG GTCTCCATGG TGGAGAAAGC ATATGCCAAG 
CTCCATGGTT CTTATGAGGC ACTGGAGGGG GGACTGGTTC AGGATGCTCT TGTCGACCTA 
ACTGGAGGAG CTGGTGAGGA GATTGACTTG CGGAGTGCTC AAGCACAAAT AGATCTTGCA 
AGTGGCAGAT TGTGGTCTCA ATTGTTACGT TTTAAACAAG AGGGGTTCTT ACTTGGTGCT 
GGAAGTCCAT CAGGATCTGA TGTTCATGTA TCTTCCAGTG GCATTGTGCA AGGGCATGCT 
TACTCCGTCT TACAGGTGAG AGAGGTTGAT GGGCACAGAC TTGTTCAGAT TCGAAATCCA 
TGGGCTAATG AAGTTGAGTG GAATGGTCCC TGGTCAGACT CATCCCCAGA GTGGACTGAT 
AGGATGAAGC ACAAGCTGAA GCATGTTCCA CAGTCAAAAG AAGGTATATT CTGGATGTCT 
TGGCAAGATT TCCAGATTCA TTTCAGATCA ATATATGTGT GTCGGGTTTA CCCCCGTGAG 
ATGCGCTACT CTGTAAATGG CCAATGGCGA GGTTATAGTG CCGGTGGCTG CCAAGATTAT 
AGCTCATGGC ATCAAAATCC ACAATTCAGG CTGAGGGCAA CTGGTTCTGA TGCATCTTTA 
CCAATTCATG TGTTCATCAC CTTAACTCAG GGCGTAGGTT TCTCGAGAAC AACTCCTGGA 
TTTCGTAACT ACCAATCAAG CCATGATTCA CAGTTGTTCT ATATCGGATT GAGGATTCTT 
AAAACTCGTG GACGTCGTGC TGCTTACAAC ATATTTCTTC ATGAATCTGT TGGTGGAACA 
GACTATGTGA ATTCCCGTGA GATTTCATGT GAAATGGTTC TTGACCCTGA TCCTAAGGGT 
TATACTATTG TCCCAACCAC GATACACCCA GGGGAAGAAG CACCTTTTGT CCTTTCAGTC 
TTCACAAAAG CATCCATTGT TCTTGAAGCT TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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DEK1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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