IFNA3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	IFNA3
Entrez Gene ID	396398
Full Name	interferon
Synonyms	IFN-alpha,IFN-gamma,IFNA,IFNA1,IFNA2,IFNA6
General protein information	Preferred Names interferon Names interferon type A1/A2 IFN alpha interferon alpha interferon type A3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205427.1	NP_990758.1	interferon type A1/A2 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100858177	XP_004937148.1
Gallus gallus (chicken)	LOC776018	XP_004937146.1
Gallus gallus (chicken)	LOC768614	XP_004937145.1
Gallus gallus (chicken)	LOC100859032	XP_004937153.1
Gallus gallus (chicken)	LOC100857403	XP_004937151.1
Gallus gallus (chicken)	LOC100858506	XP_004937149.1
Gallus gallus (chicken)	LOC100858762	XP_004937147.1
Gallus gallus (chicken)	LOC100857947	XP_004937154.1
Gallus gallus (chicken)	IFNB	NP_001020007.1
Gallus gallus (chicken)	LOC100859414	XP_004937150.1
Gallus gallus (chicken)	IFNA3	NP_990758.1
Gallus gallus (chicken)	LOC100857744	XP_004937152.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression analysis of cytosolic DNA-sensing pathway genes in the intestinal mucosal layer of necrotic enteritis-induced chicken.
Rengaraj D, Truong AD, Lee SH, Lillehoj HS, Hong YH
Veterinary immunology and immunopathology1701-12(2016 Feb)

Alpha-interferon suppresses hepadnavirus transcription by altering epigenetic modification of cccDNA minichromosomes.
Liu F, Campagna M, Qi Y, Zhao X, Guo F, Xu C, Li S, Li W, Block TM, Chang J, Guo JT
PLoS pathogens9(9)e1003613(2013 Sep)

Genetic screen of a library of chimeric poxviruses identifies an ankyrin repeat protein involved in resistance to the avian type I interferon response.
Buttigieg K, Laidlaw SM, Ross C, Davies M, Goodbourn S, Skinner MA
Journal of virology87(9)5028-40(2013 May)

Mx is dispensable for interferon-mediated resistance of chicken cells against influenza A virus.
Schusser B, Reuter A, von der Malsburg A, Penski N, Weigend S, Kaspers B, Staeheli P, H?rtle S
Journal of virology85(16)8307-15(2011 Aug)

Abundance of IFN-? and IFN-? gene transcripts and absence of IL-2 transcripts in the blood of chickens vaccinated with live or inactivated NDV.
Balenovi? M, Savi? V, Ekert Kabalin A, Jurinovi? L, Ragland WL
Acta veterinaria Hungarica59(1)141-8(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that down-regulation of circular viral DNA transcription is primary mechanism of IFN-alpha against duck hepatitis B virus; this process requires protein biosynthesis.
Title: Alpha-interferon suppresses hepadnavirus transcription by altering epigenetic modification of cccDNA minichromosomes.

Fpv014 contribute to increased resistance to chicken alpha interferon response.
Title: Genetic screen of a library of chimeric poxviruses identifies an ankyrin repeat protein involved in resistance to the avian type I interferon response.

the antiviral effect of type I IFN in chicken cells was not dependent on Mx
Title: Mx is dispensable for interferon-mediated resistance of chicken cells against influenza A virus.

The results show that cells stimulated to produce IFN-alpha and IFN-gamma enter the circulating blood but those stimulated to produce IL-2 do not, or in very low number, and the IFN responses to both vaccines are the same.
Title: Abundance of IFN-? and IFN-? gene transcripts and absence of IL-2 transcripts in the blood of chickens vaccinated with live or inactivated NDV.

The following IFNA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IFNA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa20800	NM_205427.1 Latest version!	Gallus gallus interferon (IFNA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IFNA3 ( NM_205427.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990758.1 Gallus gallus interferon (IFNA3), mRNA.

Price & Availability↑

CloneID	OGa20800
Clone ID Related Accession (Same CDS sequence)	NM_205427.1
Accession Version	NM_205427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	interferon type A1/A2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U07868.1. ##Evidence-Data-START## Transcript exon combination :: U07868.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTGTGC CTGCAAGCCC ACAGCACCCA CGGGGGTACG GCATCCTGCT GCTCACGCTC 
CTTCTGAAAG CTCTCGCCAC CACCGCCTCC GCCTGCAACC ACCTTCGCCC CCAGGATGCC 
ACCTTCTCTC ACGACAGCCT CCAGCTCCTC CGGGACATGG CTCCCACACT ACCCCAGCTG 
TGCCCACAGC ACAACGCGTC TTGCTCCTTC AACGACACCA TCCTGGACAC CAGCAACACC 
CGGCAAGCCG ACAAAACCAC CCACGACATC CTTCAGCACC TCTTCAAAAT CCTCAGCAGC 
CCCAGCACTC CAGCCCACTG GAACGACAGC CAACGCCAAA GCCTCCTCAA CCGGATCCAC 
CGCTACACCC AGCACCTCGA GCAATGCTTG GACAGCAGCG ACACGCGCTC CCGGACGCGA 
TGGCCTCGCA ACCTTCACCT CACCATCAAA AAACACTTCA GCTGCCTCCA CACCTTCCTC 
CAAGACAACG ATTACAGCGC CTGCGCCTGG GAACACGTCC GCCTGCAAGC TCGTGCCTGG 
TTCCTGCACA TCCACAACCT CACAGGCAAC ACGCGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990758.1
CDS	55..636
Translation	MAVPASPQHPRGYGILLLTLLLKALATTASACNHLRPQDATFSHDSLQLLRDMAPTLPQLCPQHNASCSFNDTILDTSNTRQADKTTHDILQHLFKILSSPSTPAHWNDSQRQSLLNRIHRYTQHLEQCLDSSDTRSRTRWPRNLHLTIKKHFSCLHTFLQDNDYSACAWEHVRLQARAWFLHIHNLTGNTRT

Target ORF information:

RefSeq Version	NM_205427.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interferon (IFNA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205427.1

ORF Insert Sequence:

ATGGCTGTGC CTGCAAGCCC ACAGCACCCA CGGGGGTACG GCATCCTGCT GCTCACGCTC 
CTTCTGAAAG CTCTCGCCAC CACCGCCTCC GCCTGCAACC ACCTTCGCCC CCAGGATGCC 
ACCTTCTCTC ACGACAGCCT CCAGCTCCTC CGGGACATGG CTCCCACACT ACCCCAGCTG 
TGCCCACAGC ACAACGCGTC TTGCTCCTTC AACGACACCA TCCTGGACAC CAGCAACACC 
CGGCAAGCCG ACAAAACCAC CCACGACATC CTTCAGCACC TCTTCAAAAT CCTCAGCAGC 
CCCAGCACTC CAGCCCACTG GAACGACAGC CAACGCCAAA GCCTCCTCAA CCGGATCCAC 
CGCTACACCC AGCACCTCGA GCAATGCTTG GACAGCAGCG ACACGCGCTC CCGGACGCGA 
TGGCCTCGCA ACCTTCACCT CACCATCAAA AAACACTTCA GCTGCCTCCA CACCTTCCTC 
CAAGACAACG ATTACAGCGC CTGCGCCTGG GAACACGTCC GCCTGCAAGC TCGTGCCTGG 
TTCCTGCACA TCCACAACCT CACAGGCAAC ACGCGCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression analysis of cytosolic DNA-sensing pathway genes in the intestinal mucosal layer of necrotic enteritis-induced chicken.
Veterinary immunology and immunopathology1701-12(2016 Feb)
Rengaraj D,Truong AD,Lee SH,Lillehoj HS,Hong YH

Alpha-interferon suppresses hepadnavirus transcription by altering epigenetic modification of cccDNA minichromosomes.
PLoS pathogens9(9)e1003613(2013 Sep)
Liu F,Campagna M,Qi Y,Zhao X,Guo F,Xu C,Li S,Li W,Block TM,Chang J,Guo JT

Genetic screen of a library of chimeric poxviruses identifies an ankyrin repeat protein involved in resistance to the avian type I interferon response.
Journal of virology87(9)5028-40(2013 May)
Buttigieg K,Laidlaw SM,Ross C,Davies M,Goodbourn S,Skinner MA

Mx is dispensable for interferon-mediated resistance of chicken cells against influenza A virus.
Journal of virology85(16)8307-15(2011 Aug)
Schusser B,Reuter A,von der Malsburg A,Penski N,Weigend S,Kaspers B,Staeheli P,H?rtle S

Abundance of IFN-? and IFN-? gene transcripts and absence of IL-2 transcripts in the blood of chickens vaccinated with live or inactivated NDV.
Acta veterinaria Hungarica59(1)141-8(2011 Mar)
Balenovi? M,Savi? V,Ekert Kabalin A,Jurinovi? L,Ragland WL