PDK3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	PDK3
Entrez Gene ID	5165
Full Name	pyruvate dehydrogenase kinase 3
Synonyms	CMTX6,GS1-358P8.4
General protein information	Preferred Names pyruvate dehydrogenase kinase 3 Names pyruvate dehydrogenase kinase, isozyme 3 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial pyruvate dehydrogenase kinase, isoenzyme 3 pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.11
Summary	The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010].
Disorder MIM:	300906
Disorder Html:	?Charcot-Marie-Tooth disease, X-linked dominant, 6, 300905 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005391.4	NP_005382.1	pyruvate dehydrogenase kinase, isozyme 3 isoform 2 precursor
NM_001142386.2	NP_001135858.1	pyruvate dehydrogenase kinase, isozyme 3 isoform 1 precursor
NM_001142386.3	NP_001135858.1	pyruvate dehydrogenase kinase, isozyme 3 isoform 1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PDK3	NP_001095404.1
Mus musculus (house mouse)	Pdk3	NP_663605.1
Danio rerio (zebrafish)	pdk3a	XP_005162814.1
Drosophila melanogaster (fruit fly)	Pdk	NP_001097240.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010276	XP_319468.3
Neurospora crassa	NCU06760	XP_963532.2
Pan troglodytes (chimpanzee)	PDK3	XP_003317444.2
	PDK3	XP_002808569.1
Rattus norvegicus (Norway rat)	Pdk3	NP_001100051.1
Caenorhabditis elegans (roundworm)	pdhk-2	NP_498928.1
Schizosaccharomyces pombe (fission yeast)	pkp1	NP_593879.1
Gallus gallus (chicken)	PDK3	NP_001006259.1
Eremothecium gossypii	AGOS_AER270W	NP_985127.2
Magnaporthe oryzae (rice blast fungus)	MGG_07161	XP_003715359.1
Oryza sativa (rice)	Os07g0637300	NP_001060401.1
Canis lupus familiaris (dog)	PDK3	XP_005641265.1
Oryza sativa (rice)	Os03g0370000	NP_001050196.1
Homo sapiens (human)	PDK3	NP_001135858.1
Kluyveromyces lactis	KLLA0F27423g	XP_456300.1
Arabidopsis thaliana (thale cress)	PDK	NP_187300.3
Xenopus tropicalis (tropical clawed frog)	pdk3	NP_001107154.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis.
Wang LY, Hung CL, Chen YR, Yang JC, Wang J, Campbell M, Izumiya Y, Chen HW, Wang WC, Ann DK, Kung HJ
Cell reports16(11)3016-3027(2016 09)

X-linked Charcot-Marie-Tooth disease type 6 (CMTX6) patients with a p.R158H mutation in the pyruvate dehydrogenase kinase isoenzyme 3 gene.
Kennerson ML, Kim EJ, Siddell A, Kidambi A, Kim SM, Hong YB, Hwang SH, Chung KW, Choi BO
Journal of the peripheral nervous system : JPNS21(1)45-51(2016 Mar)

HIF1? modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1-3 and PKM2.
Prigione A, Rohwer N, Hoffmann S, Mlody B, Drews K, Bukowiecki R, Bl?mlein K, Wanker EE, Ralser M, Cramer T, Adjaye J
Stem cells (Dayton, Ohio)32(2)364-76(2014 Feb)

A new locus for X-linked dominant Charcot-Marie-Tooth disease (CMTX6) is caused by mutations in the pyruvate dehydrogenase kinase isoenzyme 3 (PDK3) gene.
Kennerson ML, Yiu EM, Chuang DT, Kidambi A, Tso SC, Ly C, Chaudhry R, Drew AP, Rance G, Delatycki MB, Z?chner S, Ryan MM, Nicholson GA
Human molecular genetics22(7)1404-16(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The pyruvate dehydrogenase kinases (PDKs) PDK1 and PDK3 are direct targets of KDM4A and E2F1 and modulate the switch between glycolytic metabolism and mitochondrial oxidation.
Title: KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis.

We have identified a p.R158H PDK3 mutation in a Korean X-linked dominant Charcot-Marie-Tooth type 6 family
Title: X-linked Charcot-Marie-Tooth disease type 6 (CMTX6) patients with a p.R158H mutation in the pyruvate dehydrogenase kinase isoenzyme 3 gene.

Accordingly, elevated levels of PDK1, PDK3, and PKM2 and reduced PK activity could be observed in iPSCs and human embryonic stem cells in the undifferentiated state
Title: HIF1? modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1-3 and PKM2.

Reduced pyruvate flux due to R158H mutant PDK3-mediated hyper-phosphorylation of the pyruvate dehydrogenase complex is the underlying pathogenic cause of peripheral neuropathy in X-linked dominant Charcot-Marie-Tooth disease.
Title: A new locus for X-linked dominant Charcot-Marie-Tooth disease (CMTX6) is caused by mutations in the pyruvate dehydrogenase kinase isoenzyme 3 (PDK3) gene.

Findings suggest the HIF-1/PDK3 bioenergetic pathway as a new target for therapeutic intervention in metastatic melanoma.
Title: Inactivation of the HIF-1?/PDK3 signaling axis drives melanoma toward mitochondrial oxidative metabolism and potentiates the therapeutic activity of pro-oxidants.

The following PDK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21847	NM_005391.4 Latest version!	Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20872	NM_001142386.3 Latest version!	Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$244.30 $349.00
View Old Accession Versions >>
OHu20872	NM_001142386.2	Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$244.30 $349.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDK3 ( NM_005391.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005382.1 Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 2, mRNA.

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CloneID	OHu21847
Clone ID Related Accession (Same CDS sequence)	NM_005391.4
Accession Version	NM_005391.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 3 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI549903.1, BC015948.1, L42452.1 and AC004656.1. This sequence is a reference standard in the RefSeqGene project. On Mar 13, 2010 this sequence version replaced NM_005391.3. Transcript Variant: This variant (2) differs at the 3' end compared to variant 1, resulting in a shorter isoform (2) lacking the last 9 aa from the C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L42452.1, SRR1803615.127858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGT TCCGGTGGCT GCTGAAGCAG CCGGTGCCCA AGCAGATCGA GCGCTACTCG 
CGCTTTTCGC CGTCGCCGCT CTCCATCAAA CAATTCCTGG ACTTCGGGAG AGATAATGCA 
TGTGAGAAAA CTTCATATAT GTTTCTACGA AAGGAACTTC CTGTGCGGCT GGCTAACACA 
ATGAGAGAAG TTAATCTTCT GCCGGATAAT TTACTTAACC GCCCTTCAGT GGGATTGGTT 
CAGAGTTGGT ATATGCAGAG TTTTCTTGAA CTTTTAGAAT ATGAAAATAA GAGCCCTGAG 
GATCCACAGG TCTTGGATAA CTTTCTACAA GTTCTGATTA AAGTCAGAAA TAGACACAAT 
GATGTGGTTC CTACAATGGC ACAAGGAGTG ATTGAATACA AGGAGAAGTT TGGGTTTGAT 
CCTTTCATTA GCACTAACAT CCAATATTTT CTGGATCGGT TTTATACCAA CCGCATCTCT 
TTCCGCATGC TTATTAATCA GCACACACTT CTGTTTGGGG GTGACACTAA TCCTGTTCAT 
CCTAAACACA TAGGAAGTAT CGATCCCACC TGTAACGTGG CGGATGTGGT GAAAGATGCA 
TATGAAACAG CCAAGATGCT GTGTGAACAG TATTACCTGG TAGCTCCAGA GCTGGAAGTT 
GAAGAATTCA ATGCCAAAGC GCCAGACAAA CCTATTCAGG TGGTTTATGT GCCCTCACAT 
CTGTTTCATA TGCTATTTGA GTTGTTCAAG AACTCAATGA GAGCGACAGT TGAACTCTAT 
GAAGACAGAA AAGAGGGCTA CCCTGCTGTT AAAACCCTCG TTACTTTGGG TAAAGAAGAC 
TTATCCATTA AGATCAGTGA CCTAGGTGGT GGTGTCCCAC TTCGAAAAAT AGATCGTCTT 
TTTAACTACA TGTATTCTAC TGCTCCTAGA CCCAGCCTGG AGCCTACCAG AGCTGCCCCT 
TTGGCTGGAT TTGGTTATGG TTTGCCAATT TCCCGTCTGT ATGCTAGATA TTTTCAAGGA 
GATCTGAAAC TGTATTCCAT GGAAGGAGTG GGTACTGATG CTGTCATTTA TTTGAAGGCT 
CTTTCAAGTG AGTCATTTGA GAGACTTCCA GTTTTTAATA AGTCCGCATG GCGCCATTAC 
AAGACCACGC CTGAAGCCGA TGATTGGAGC AATCCCAGCA GTGAACCCAG GGATGCTTCA 
AAATACAAAG CAAAACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005382.1
CDS	230..1450
Translation	MRLFRWLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQ

Target ORF information:

RefSeq Version	NM_005391.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005391.4

ORF Insert Sequence:

ATGCGGCTGT TCCGGTGGCT GCTGAAGCAG CCGGTGCCCA AGCAGATCGA GCGCTACTCG 
CGCTTTTCGC CGTCGCCGCT CTCCATCAAA CAATTCCTGG ACTTCGGGAG AGATAATGCA 
TGTGAGAAAA CTTCATATAT GTTTCTACGA AAGGAACTTC CTGTGCGGCT GGCTAACACA 
ATGAGAGAAG TTAATCTTCT GCCGGATAAT TTACTTAACC GCCCTTCAGT GGGATTGGTT 
CAGAGTTGGT ATATGCAGAG TTTTCTTGAA CTTTTAGAAT ATGAAAATAA GAGCCCTGAG 
GATCCACAGG TCTTGGATAA CTTTCTACAA GTTCTGATTA AAGTCAGAAA TAGACACAAT 
GATGTGGTTC CTACAATGGC ACAAGGAGTG ATTGAATACA AGGAGAAGTT TGGGTTTGAT 
CCTTTCATTA GCACTAACAT CCAATATTTT CTGGATCGGT TTTATACCAA CCGCATCTCT 
TTCCGCATGC TTATTAATCA GCACACACTT CTGTTTGGGG GTGACACTAA TCCTGTTCAT 
CCTAAACACA TAGGAAGTAT CGATCCCACC TGTAACGTGG CGGATGTGGT GAAAGATGCA 
TATGAAACAG CCAAGATGCT GTGTGAACAG TATTACCTGG TAGCTCCAGA GCTGGAAGTT 
GAAGAATTCA ATGCCAAAGC GCCAGACAAA CCTATTCAGG TGGTTTATGT GCCCTCACAT 
CTGTTTCATA TGCTATTTGA GTTGTTCAAG AACTCAATGA GAGCGACAGT TGAACTCTAT 
GAAGACAGAA AAGAGGGCTA CCCTGCTGTT AAAACCCTCG TTACTTTGGG TAAAGAAGAC 
TTATCCATTA AGATCAGTGA CCTAGGTGGT GGTGTCCCAC TTCGAAAAAT AGATCGTCTT 
TTTAACTACA TGTATTCTAC TGCTCCTAGA CCCAGCCTGG AGCCTACCAG AGCTGCCCCT 
TTGGCTGGAT TTGGTTATGG TTTGCCAATT TCCCGTCTGT ATGCTAGATA TTTTCAAGGA 
GATCTGAAAC TGTATTCCAT GGAAGGAGTG GGTACTGATG CTGTCATTTA TTTGAAGGCT 
CTTTCAAGTG AGTCATTTGA GAGACTTCCA GTTTTTAATA AGTCCGCATG GCGCCATTAC 
AAGACCACGC CTGAAGCCGA TGATTGGAGC AATCCCAGCA GTGAACCCAG GGATGCTTCA 
AAATACAAAG CAAAACAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDK3 ( NM_001142386.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135858.1 Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20872
Clone ID Related Accession (Same CDS sequence)	NM_001142386.2 , NM_001142386.3
Accession Version	NM_001142386.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI549903.1, AK301965.1, BU069975.1, AC004656.1, BC038512.2 and AI001064.1. On Mar 13, 2010 this sequence version replaced NM_001142386.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.257981.1, SRR1803614.215677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGT TCCGGTGGCT GCTGAAGCAG CCGGTGCCCA AGCAGATCGA GCGCTACTCG 
CGCTTTTCGC CGTCGCCGCT CTCCATCAAA CAATTCCTGG ACTTCGGGAG AGATAATGCA 
TGTGAGAAAA CTTCATATAT GTTTCTACGA AAGGAACTTC CTGTGCGGCT GGCTAACACA 
ATGAGAGAAG TTAATCTTCT GCCGGATAAT TTACTTAACC GCCCTTCAGT GGGATTGGTT 
CAGAGTTGGT ATATGCAGAG TTTTCTTGAA CTTTTAGAAT ATGAAAATAA GAGCCCTGAG 
GATCCACAGG TCTTGGATAA CTTTCTACAA GTTCTGATTA AAGTCAGAAA TAGACACAAT 
GATGTGGTTC CTACAATGGC ACAAGGAGTG ATTGAATACA AGGAGAAGTT TGGGTTTGAT 
CCTTTCATTA GCACTAACAT CCAATATTTT CTGGATCGGT TTTATACCAA CCGCATCTCT 
TTCCGCATGC TTATTAATCA GCACACACTT CTGTTTGGGG GTGACACTAA TCCTGTTCAT 
CCTAAACACA TAGGAAGTAT CGATCCCACC TGTAACGTGG CGGATGTGGT GAAAGATGCA 
TATGAAACAG CCAAGATGCT GTGTGAACAG TATTACCTGG TAGCTCCAGA GCTGGAAGTT 
GAAGAATTCA ATGCCAAAGC GCCAGACAAA CCTATTCAGG TGGTTTATGT GCCCTCACAT 
CTGTTTCATA TGCTATTTGA GTTGTTCAAG AACTCAATGA GAGCGACAGT TGAACTCTAT 
GAAGACAGAA AAGAGGGCTA CCCTGCTGTT AAAACCCTCG TTACTTTGGG TAAAGAAGAC 
TTATCCATTA AGATCAGTGA CCTAGGTGGT GGTGTCCCAC TTCGAAAAAT AGATCGTCTT 
TTTAACTACA TGTATTCTAC TGCTCCTAGA CCCAGCCTGG AGCCTACCAG AGCTGCCCCT 
TTGGCTGGAT TTGGTTATGG TTTGCCAATT TCCCGTCTGT ATGCTAGATA TTTTCAAGGA 
GATCTGAAAC TGTATTCCAT GGAAGGAGTG GGTACTGATG CTGTCATTTA TTTGAAGGCT 
CTTTCAAGTG AGTCATTTGA GAGACTTCCA GTTTTTAATA AGTCCGCATG GCGCCATTAC 
AAGACCACGC CTGAAGCCGA TGATTGGAGC AATCCCAGCA GTGAACCCAG GGATGCTTCA 
AAATACAAAG CAAAACAAGA CAAGATCAAG ACTAATAGAA CTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135858.1
CDS	230..1477
Translation	MRLFRWLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQDKIKTNRTF

Target ORF information:

RefSeq Version	NM_001142386.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142386.2

ORF Insert Sequence:

ATGCGGCTGT TCCGGTGGCT GCTGAAGCAG CCGGTGCCCA AGCAGATCGA GCGCTACTCG 
CGCTTTTCGC CGTCGCCGCT CTCCATCAAA CAATTCCTGG ACTTCGGGAG AGATAATGCA 
TGTGAGAAAA CTTCATATAT GTTTCTACGA AAGGAACTTC CTGTGCGGCT GGCTAACACA 
ATGAGAGAAG TTAATCTTCT GCCGGATAAT TTACTTAACC GCCCTTCAGT GGGATTGGTT 
CAGAGTTGGT ATATGCAGAG TTTTCTTGAA CTTTTAGAAT ATGAAAATAA GAGCCCTGAG 
GATCCACAGG TCTTGGATAA CTTTCTACAA GTTCTGATTA AAGTCAGAAA TAGACACAAT 
GATGTGGTTC CTACAATGGC ACAAGGAGTG ATTGAATACA AGGAGAAGTT TGGGTTTGAT 
CCTTTCATTA GCACTAACAT CCAATATTTT CTGGATCGGT TTTATACCAA CCGCATCTCT 
TTCCGCATGC TTATTAATCA GCACACACTT CTGTTTGGGG GTGACACTAA TCCTGTTCAT 
CCTAAACACA TAGGAAGTAT CGATCCCACC TGTAACGTGG CGGATGTGGT GAAAGATGCA 
TATGAAACAG CCAAGATGCT GTGTGAACAG TATTACCTGG TAGCTCCAGA GCTGGAAGTT 
GAAGAATTCA ATGCCAAAGC GCCAGACAAA CCTATTCAGG TGGTTTATGT GCCCTCACAT 
CTGTTTCATA TGCTATTTGA GTTGTTCAAG AACTCAATGA GAGCGACAGT TGAACTCTAT 
GAAGACAGAA AAGAGGGCTA CCCTGCTGTT AAAACCCTCG TTACTTTGGG TAAAGAAGAC 
TTATCCATTA AGATCAGTGA CCTAGGTGGT GGTGTCCCAC TTCGAAAAAT AGATCGTCTT 
TTTAACTACA TGTATTCTAC TGCTCCTAGA CCCAGCCTGG AGCCTACCAG AGCTGCCCCT 
TTGGCTGGAT TTGGTTATGG TTTGCCAATT TCCCGTCTGT ATGCTAGATA TTTTCAAGGA 
GATCTGAAAC TGTATTCCAT GGAAGGAGTG GGTACTGATG CTGTCATTTA TTTGAAGGCT 
CTTTCAAGTG AGTCATTTGA GAGACTTCCA GTTTTTAATA AGTCCGCATG GCGCCATTAC 
AAGACCACGC CTGAAGCCGA TGATTGGAGC AATCCCAGCA GTGAACCCAG GGATGCTTCA 
AAATACAAAG CAAAACAAGA CAAGATCAAG ACTAATAGAA CTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDK3 ( NM_001142386.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135858.1 Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20872
Clone ID Related Accession (Same CDS sequence)	NM_001142386.2 , NM_001142386.3
Accession Version	NM_001142386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 3 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI549903.1, AK301965.1, BU069975.1, AC004656.1, BC038512.2 and AI001064.1. On Jun 2, 2019 this sequence version replaced NM_001142386.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.145761.1, SRR1803614.215677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGCTGT TCCGGTGGCT GCTGAAGCAG CCGGTGCCCA AGCAGATCGA GCGCTACTCG 
CGCTTTTCGC CGTCGCCGCT CTCCATCAAA CAATTCCTGG ACTTCGGGAG AGATAATGCA 
TGTGAGAAAA CTTCATATAT GTTTCTACGA AAGGAACTTC CTGTGCGGCT GGCTAACACA 
ATGAGAGAAG TTAATCTTCT GCCGGATAAT TTACTTAACC GCCCTTCAGT GGGATTGGTT 
CAGAGTTGGT ATATGCAGAG TTTTCTTGAA CTTTTAGAAT ATGAAAATAA GAGCCCTGAG 
GATCCACAGG TCTTGGATAA CTTTCTACAA GTTCTGATTA AAGTCAGAAA TAGACACAAT 
GATGTGGTTC CTACAATGGC ACAAGGAGTG ATTGAATACA AGGAGAAGTT TGGGTTTGAT 
CCTTTCATTA GCACTAACAT CCAATATTTT CTGGATCGGT TTTATACCAA CCGCATCTCT 
TTCCGCATGC TTATTAATCA GCACACACTT CTGTTTGGGG GTGACACTAA TCCTGTTCAT 
CCTAAACACA TAGGAAGTAT CGATCCCACC TGTAACGTGG CGGATGTGGT GAAAGATGCA 
TATGAAACAG CCAAGATGCT GTGTGAACAG TATTACCTGG TAGCTCCAGA GCTGGAAGTT 
GAAGAATTCA ATGCCAAAGC GCCAGACAAA CCTATTCAGG TGGTTTATGT GCCCTCACAT 
CTGTTTCATA TGCTATTTGA GTTGTTCAAG AACTCAATGA GAGCGACAGT TGAACTCTAT 
GAAGACAGAA AAGAGGGCTA CCCTGCTGTT AAAACCCTCG TTACTTTGGG TAAAGAAGAC 
TTATCCATTA AGATCAGTGA CCTAGGTGGT GGTGTCCCAC TTCGAAAAAT AGATCGTCTT 
TTTAACTACA TGTATTCTAC TGCTCCTAGA CCCAGCCTGG AGCCTACCAG AGCTGCCCCT 
TTGGCTGGAT TTGGTTATGG TTTGCCAATT TCCCGTCTGT ATGCTAGATA TTTTCAAGGA 
GATCTGAAAC TGTATTCCAT GGAAGGAGTG GGTACTGATG CTGTCATTTA TTTGAAGGCT 
CTTTCAAGTG AGTCATTTGA GAGACTTCCA GTTTTTAATA AGTCCGCATG GCGCCATTAC 
AAGACCACGC CTGAAGCCGA TGATTGGAGC AATCCCAGCA GTGAACCCAG GGATGCTTCA 
AAATACAAAG CAAAACAAGA CAAGATCAAG ACTAATAGAA CTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135858.1
CDS	171..1418
Translation	MRLFRWLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQDKIKTNRTF

Target ORF information:

RefSeq Version	NM_001142386.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 3 (PDK3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142386.3

ORF Insert Sequence:

ATGCGGCTGT TCCGGTGGCT GCTGAAGCAG CCGGTGCCCA AGCAGATCGA GCGCTACTCG 
CGCTTTTCGC CGTCGCCGCT CTCCATCAAA CAATTCCTGG ACTTCGGGAG AGATAATGCA 
TGTGAGAAAA CTTCATATAT GTTTCTACGA AAGGAACTTC CTGTGCGGCT GGCTAACACA 
ATGAGAGAAG TTAATCTTCT GCCGGATAAT TTACTTAACC GCCCTTCAGT GGGATTGGTT 
CAGAGTTGGT ATATGCAGAG TTTTCTTGAA CTTTTAGAAT ATGAAAATAA GAGCCCTGAG 
GATCCACAGG TCTTGGATAA CTTTCTACAA GTTCTGATTA AAGTCAGAAA TAGACACAAT 
GATGTGGTTC CTACAATGGC ACAAGGAGTG ATTGAATACA AGGAGAAGTT TGGGTTTGAT 
CCTTTCATTA GCACTAACAT CCAATATTTT CTGGATCGGT TTTATACCAA CCGCATCTCT 
TTCCGCATGC TTATTAATCA GCACACACTT CTGTTTGGGG GTGACACTAA TCCTGTTCAT 
CCTAAACACA TAGGAAGTAT CGATCCCACC TGTAACGTGG CGGATGTGGT GAAAGATGCA 
TATGAAACAG CCAAGATGCT GTGTGAACAG TATTACCTGG TAGCTCCAGA GCTGGAAGTT 
GAAGAATTCA ATGCCAAAGC GCCAGACAAA CCTATTCAGG TGGTTTATGT GCCCTCACAT 
CTGTTTCATA TGCTATTTGA GTTGTTCAAG AACTCAATGA GAGCGACAGT TGAACTCTAT 
GAAGACAGAA AAGAGGGCTA CCCTGCTGTT AAAACCCTCG TTACTTTGGG TAAAGAAGAC 
TTATCCATTA AGATCAGTGA CCTAGGTGGT GGTGTCCCAC TTCGAAAAAT AGATCGTCTT 
TTTAACTACA TGTATTCTAC TGCTCCTAGA CCCAGCCTGG AGCCTACCAG AGCTGCCCCT 
TTGGCTGGAT TTGGTTATGG TTTGCCAATT TCCCGTCTGT ATGCTAGATA TTTTCAAGGA 
GATCTGAAAC TGTATTCCAT GGAAGGAGTG GGTACTGATG CTGTCATTTA TTTGAAGGCT 
CTTTCAAGTG AGTCATTTGA GAGACTTCCA GTTTTTAATA AGTCCGCATG GCGCCATTAC 
AAGACCACGC CTGAAGCCGA TGATTGGAGC AATCCCAGCA GTGAACCCAG GGATGCTTCA 
AAATACAAAG CAAAACAAGA CAAGATCAAG ACTAATAGAA CTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis.
Cell reports16(11)3016-3027(2016 09)
Wang LY,Hung CL,Chen YR,Yang JC,Wang J,Campbell M,Izumiya Y,Chen HW,Wang WC,Ann DK,Kung HJ

X-linked Charcot-Marie-Tooth disease type 6 (CMTX6) patients with a p.R158H mutation in the pyruvate dehydrogenase kinase isoenzyme 3 gene.
Journal of the peripheral nervous system : JPNS21(1)45-51(2016 Mar)
Kennerson ML,Kim EJ,Siddell A,Kidambi A,Kim SM,Hong YB,Hwang SH,Chung KW,Choi BO

HIF1? modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1-3 and PKM2.
Stem cells (Dayton, Ohio)32(2)364-76(2014 Feb)
Prigione A,Rohwer N,Hoffmann S,Mlody B,Drews K,Bukowiecki R,Bl?mlein K,Wanker EE,Ralser M,Cramer T,Adjaye J

A new locus for X-linked dominant Charcot-Marie-Tooth disease (CMTX6) is caused by mutations in the pyruvate dehydrogenase kinase isoenzyme 3 (PDK3) gene.
Human molecular genetics22(7)1404-16(2013 Apr)
Kennerson ML,Yiu EM,Chuang DT,Kidambi A,Tso SC,Ly C,Chaudhry R,Drew AP,Rance G,Delatycki MB,Z?chner S,Ryan MM,Nicholson GA

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PDK3 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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