CNGB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	CNGB1
Entrez Gene ID	1258
Full Name	cyclic nucleotide gated channel beta 1
Synonyms	CNCG2,CNCG3L,CNCG4,CNG4,CNGB1B,GAR1,GARP,GARP2,RCNC2,RCNCb,RCNCbeta,RP45
General protein information	Preferred Names cyclic nucleotide gated channel beta 1 Names cyclic nucleotide-gated cation channel beta-1 CNG channel beta-1 CNG-4 cyclic nucleotide-gated cation channel 4 cyclic nucleotide-gated cation channel gamma cyclic nucleotide-gated cation channel modulatory subunit glutamic-acid-rich protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q21
Summary	In humans, the rod photoreceptor cGMP-gated cation channel helps regulate ion flow into the rod photoreceptor outer segment in response to light-induced alteration of the levels of intracellular cGMP. This channel consists of two subunits, alpha and beta, with the protein encoded by this gene representing the beta subunit. Defects in this gene are a cause of cause of retinitis pigmentosa type 45. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013].
Disorder MIM:	600724
Disorder Html:	Retinitis pigmentosa 45, 613767 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297.4	NP_001288.3	cyclic nucleotide-gated cation channel beta-1 isoform a
NM_001286130.1	NP_001273059.1	cyclic nucleotide-gated cation channel beta-1 isoform c
NM_001135639.1	NP_001129111.1	cyclic nucleotide-gated cation channel beta-1 isoform b
NM_001297.5	NP_001288.3	cyclic nucleotide-gated cation channel beta-1 isoform a
NM_001286130.2	NP_001273059.1	cyclic nucleotide-gated cation channel beta-1 isoform c
NM_001135639.2	NP_001129111.1	cyclic nucleotide-gated cation channel beta-1 isoform b
XM_011522870.2	XP_011521172.1	cyclic nucleotide-gated cation channel beta-1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CNGB1	XP_001231878.3
Caenorhabditis elegans (roundworm)	tax-2	NP_492427.3
Homo sapiens (human)	CNGB1	NP_001273059.1
	CNGB1	XP_001100587.2
Pan troglodytes (chimpanzee)	CNGB1	XP_510998.3
Bos taurus (cattle)	CNGB1	NP_851362.1
Canis lupus familiaris (dog)	CNGB1	XP_005617564.1
Rattus norvegicus (Norway rat)	Cngb1	NP_113997.1

Gene Ontology
Bibliography

Related articles in PubMed

Patients and animal models of CNG?1-deficient retinitis pigmentosa support gene augmentation approach.
Petersen-Jones SM, Occelli LM, Winkler PA, Lee W, Sparrow JR, Tsukikawa M, Boye SL, Chiodo V, Capasso JE, Becirovic E, Sch?n C, Seeliger MW, Levin AV, Michalakis S, Hauswirth WW, Tsang SH
The Journal of clinical investigation128(1)190-206(2018 Jan)

Run of homozygosity analysis reveals a novel nonsense variant of the CNGB1 gene involved in retinitis pigmentosa 45.
Fradin M, Colin E, Hannouche-Bared D, Audo I, Sahel JA, Biskup S, Carr? W, Ziegler A, Wilhelm C, Guichet A, Odent S, Bonneau D
Ophthalmic genetics37(3)357-9(2016 09)

Varying the GARP2-to-RDS Ratio Leads to Defects in Rim Formation and Rod and Cone Function.
Chakraborty D, Conley SM, DeRamus ML, Pittler SJ, Naash MI
Investigative ophthalmology & visual science56(13)8187-98(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, we present the first case of RP45 caused by an unequivocal homozygous nonsense variant of CNGB1 identified by run of homozygosity (ROH) analysis.
Title: Run of homozygosity analysis reveals a novel nonsense variant of the CNGB1 gene involved in retinitis pigmentosa 45.

GARP2 expression in cones can be detrimental to cones. RDS/GARP interactions remain under investigation but are critical for both OS structure and function.
Title: Varying the GARP2-to-RDS Ratio Leads to Defects in Rim Formation and Rod and Cone Function.

Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP, and FoxP3+Helios+ Tregs have more suppressive characteristics, compared with FoxP3+Helios- Tregs.
Title: Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP.

The p.Arg86Gln mutation actually appears to be a polymorphism common in ethnic West Africans and not associated with RP. This change may provide a useful marker for West African ancestry.
Title: The p.Arg86Gln change in GARP2 (glutamic acid-rich protein-2) is a common West African-related polymorphism.

Deletion of the cyclic nucleotide gated channel CNGB1 alters response waveform but leaves light adaptation intact in a transgenic model.
Title: Channel modulation and the mechanism of light adaptation in mouse rods.

The following CNGB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNGB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16506	NM_001135639.2 Latest version!	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu18553	NM_001297.5 Latest version!	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu21806	NM_001286130.2 Latest version!	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu56956	XM_011522870.2 Latest version!	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu18553	NM_001297.4	Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu21806	NM_001286130.1	Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu16506	NM_001135639.1	Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNGB1 ( NM_001135639.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129111.1 Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 2, mRNA.

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CloneID	OHu16506
Clone ID Related Accession (Same CDS sequence)	NM_001135639.1 , NM_001135639.2
Accession Version	NM_001135639.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U18945.1, BQ638084.1, AC010543.9, EL945866.1 and BM672421.1. Transcript Variant: This variant (2) uses an alternate exon and lacks the 3' coding region, compared to variant 1. The resulting isoform (b), also known as GARP2, is much shorter and has a unique C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U18945.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142853 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCAGGGTGA TGGGAGCTGG AGGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129111.1
CDS	66..965
Translation	MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPSPQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCTDEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATGAASDPAPPGRPQEMGPKLQARETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQEPDSPGICDVQTRVMGAGGL

Target ORF information:

RefSeq Version	NM_001135639.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135639.1

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCAGGGTGA TGGGAGCTGG AGGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB1 ( NM_001135639.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129111.1 Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 2, mRNA.

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CloneID	OHu16506
Clone ID Related Accession (Same CDS sequence)	NM_001135639.1 , NM_001135639.2
Accession Version	NM_001135639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010543.9, U18945.1, BQ638084.1, EL945866.1 and BM672421.1. On May 31, 2019 this sequence version replaced NM_001135639.1. Transcript Variant: This variant (2) uses an alternate exon and lacks the 3' coding region, compared to variant 1. The resulting isoform (b), also known as GARP2, is much shorter and has a unique C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U18945.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142853 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCAGGGTGA TGGGAGCTGG AGGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129111.1
CDS	78..977
Translation	MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPSPQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCTDEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATGAASDPAPPGRPQEMGPKLQARETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQEPDSPGICDVQTRVMGAGGL

Target ORF information:

RefSeq Version	NM_001135639.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135639.2

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCAGGGTGA TGGGAGCTGG AGGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB1 ( NM_001297.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288.3 Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18553
Clone ID Related Accession (Same CDS sequence)	NM_001297.4 , NM_001297.5
Accession Version	NM_001297.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U18945.1, U58837.1, AC010543.9 and BM678849.1. This sequence is a reference standard in the RefSeqGene project. On Oct 3, 2008 this sequence version replaced NM_001297.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U58837.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCATCAGCA TCCTTCCTGG AGGACAAGTG 
GAGCCTGACC TTGTCCTAGA GGAGGTTGAA CCGCCCTGGG AGGATGCCCA CCAGGATGTC 
AGTACCAGCC CACAGGGTAC AGAGGTGGTT CCAGCTTATG AAGAAGAGAA CAAAGCTGTG 
GAGAAGATGC CCAGAGAGCT GTCCCGGATT GAAGAGGAGA AAGAAGATGA GGAGGAGGAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGACTG AGGTGCTGCT GGATAGCTGT 
GTGGTGTCGC AGGTGGGCGT GGGCCAGAGT GAAGAAGACG GGACCCGGCC CCAGAGCACT 
TCAGATCAGA AGCTGTGGGA GGAAGTTGGG GAGGAGGCCA AGAAGGAGGC TGAAGAGAAG 
GCCAAGGAGG AGGCCGAGGA GGTGGCTGAA GAGGAGGCTG AAAAGGAGCC CCAGGACTGG 
GCGGAGACCA AGGAGGAGCC TGAGGCTGAG GCCGAGGCTG CCAGTTCAGG AGTGCCTGCC 
ACGAAACAGC ACCCAGAAGT GCAGGTGGAA GATACTGATG CTGATAGCTG CCCCCTCATG 
GCAGAAGAGA ATCCACCCTC AACCGTGTTG CCGCCACCGT CTCCTGCCAA ATCAGACACC 
CTTATAGTCC CAAGCTCAGC CTCGGGGACA CACAGGAAGA AGCTGCCCTC TGAGGATGAT 
GAGGCTGAAG AGCTCAAGGC GTTGTCACCA GCAGAGTCCC CAGTGGTTGC CTGGTCTGAC 
CCCACCACCC CGAAGGACAC TGATGGCCAG GACCGTGCGG CCTCCACGGC CAGCACAAAT 
AGCGCCATCA TCAACGACCG GCTCCAGGAG CTGGTGAAGC TCTTCAAGGA GCGGACAGAG 
AAAGTGAAGG AGAAACTCAT TGACCCTGAC GTCACCTCTG ATGAGGAGAG CCCCAAGCCC 
TCCCCAGCCA AGAAAGCCCC AGAGCCAGCT CCAGACACAA AGCCCGCTGA AGCCGAGCCA 
GTGGAAGAGG AGCACTATTG CGACATGCTC TGCTGCAAGT TCAAACACCG CCCCTGGAAG 
AAGTACCAGT TTCCCCAGAG CATTGACCCG CTGACCAACC TGATGTATGT CCTATGGCTG 
TTCTTCGTGG TGATGGCCTG GAATTGGAAC TGTTGGCTGA TTCCCGTGCG CTGGGCCTTC 
CCCTACCAGA CCCCGGACAA CATCCACCAC TGGCTGCTGA TGGATTACCT ATGCGACCTC 
ATCTACTTCC TGGACATCAC CGTGTTCCAG ACACGCCTGC AGTTTGTCAG AGGCGGGGAC 
ATCATTACGG ACAAAAAGGA CATGCGAAAT AACTACCTGA AGTCTCGCCG CTTCAAGATG 
GACCTGCTCA GCCTCCTGCC CTTGGATTTT CTCTATTTGA AAGTCGGTGT GAACCCCCTC 
CTCCGCCTGC CCCGCTGTTT AAAGTACATG GCCTTCTTCG AGTTTAACAG CCGCCTGGAA 
TCCATCCTCA GCAAAGCCTA CGTGTACAGG GTCATCAGGA CCACAGCCTA CCTTCTCTAC 
AGCCTGCATT TGAATTCCTG TCTTTATTAC TGGGCATCGG CCTATCAGGG CCTCGGCTCC 
ACTCACTGGG TTTACGATGG CGTGGGAAAC AGTTATATTC GCTGTTACTA CTTTGCTGTG 
AAGACCCTCA TCACCATCGG GGGGCTGCCT GACCCCAAGA CACTCTTTGA AATTGTCTTC 
CAGCTGCTGA ATTATTTCAC GGGCGTCTTT GCTTTCTCTG TGATGATCGG ACAGATGAGA 
GATGTGGTAG GGGCCGCCAC CGCCGGACAG ACCTACTACC GCAGCTGCAT GGACAGCACG 
GTGAAGTACA TGAATTTCTA CAAGATCCCC AAGTCCGTGC AGAACCGCGT CAAGACCTGG 
TACGAGTACA CCTGGCACTC GCAAGGCATG CTGGATGAGT CAGAGCTGAT GGTGCAGCTT 
CCAGACAAGA TGCGGCTGGA CCTCGCCATC GACGTGAACT ACAACATCGT TAGCAAAGTC 
GCACTCTTTC AGGGCTGTGA CCGGCAGATG ATCTTTGACA TGCTGAAGAG GCTTCGCTCT 
GTTGTCTACC TGCCCAACGA CTATGTGTGC AAGAAGGGGG AGATCGGCCG TGAGATGTAC 
ATCATCCAGG CAGGGCAAGT GCAGGTCTTG GGCGGCCCTG ATGGGAAATC TGTGCTGGTG 
ACGCTGAAAG CTGGATCTGT GTTTGGAGAA ATAAGCTTGC TGGCTGTTGG GGGCGGGAAC 
CGGCGCACGG CCAACGTGGT GGCGCACGGG TTTACCAACC TCTTCATCCT GGATAAGAAG 
GACCTGAATG AGATTTTGGT GCATTATCCT GAGTCTCAGA AGTTACTCCG GAAGAAAGCC 
AGGCGCATGC TGAGAAGCAA CAATAAGCCC AAGGAGGAGA AGAGCGTGCT GATCCTTCCA 
CCCCGGGCGG GCACCCCAAA GCTCTTCAAC GCTGCCCTCG CTATGACAGG AAAGATGGGT 
GGCAAGGGGG CAAAAGGCGG CAAACTTGCT CACCTCCGGG CCCGGCTCAA AGAACTGGCC 
GCGCTGGAGG CGGCTGCAAA GCAGCAAGAG TTGGTGGAAC AGGCCAAGAG CTCGCAAGAC 
GTCAAGGGAG AGGAAGGCTC CGCCGCCCCA GACCAGCACA CGCACCCAAA GGAGGCCGCC 
ACCGACCCAC CCGCGCCCCG GACGCCCCCC GAGCCCCCGG GGTCTCCACC GAGCTCTCCA 
CCGCCTGCCT CCCTTGGGAG GCCGGAGGGA GAGGAGGAGG GGCCGGCCGA GCCCGAAGAG 
CACTCGGTGA GGATCTGCAT GAGCCCGGGC CCGGAGCCGG GAGAGCAGAT CCTGTCGGTG 
AAGATGCCGG AGGAAAGGGA GGAGAAGGCG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288.3
CDS	66..3821
Translation	MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPSPQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCTDEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATGAASDPAPPGRPQEMGPKLQARETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQEPDSPGICDVQTISILPGGQVEPDLVLEEVEPPWEDAHQDVSTSPQGTEVVPAYEEENKAVEKMPRELSRIEEEKEDEEEEEEEEEEEEEEEVTEVLLDSCVVSQVGVGQSEEDGTRPQSTSDQKLWEEVGEEAKKEAEEKAKEEAEEVAEEEAEKEPQDWAETKEEPEAEAEAASSGVPATKQHPEVQVEDTDADSCPLMAEENPPSTVLPPPSPAKSDTLIVPSSASGTHRKKLPSEDDEAEELKALSPAESPVVAWSDPTTPKDTDGQDRAASTASTNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPDTKPAEAEPVEEEHYCDMLCCKFKHRPWKKYQFPQSIDPLTNLMYVLWLFFVVMAWNWNCWLIPVRWAFPYQTPDNIHHWLLMDYLCDLIYFLDITVFQTRLQFVRGGDIITDKKDMRNNYLKSRRFKMDLLSLLPLDFLYLKVGVNPLLRLPRCLKYMAFFEFNSRLESILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAYQGLGSTHWVYDGVGNSYIRCYYFAVKTLITIGGLPDPKTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPKSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYNIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKSVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRSNNKPKEEKSVLILPPRAGTPKLFNAALAMTGKMGGKGAKGGKLAHLRARLKELAALEAAAKQQELVEQAKSSQDVKGEEGSAAPDQHTHPKEAATDPPAPRTPPEPPGSPPSSPPPASLGRPEGEEEGPAEPEEHSVRICMSPGPEPGEQILSVKMPEEREEKAE

Target ORF information:

RefSeq Version	NM_001297.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297.4

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCATCAGCA TCCTTCCTGG AGGACAAGTG 
GAGCCTGACC TTGTCCTAGA GGAGGTTGAA CCGCCCTGGG AGGATGCCCA CCAGGATGTC 
AGTACCAGCC CACAGGGTAC AGAGGTGGTT CCAGCTTATG AAGAAGAGAA CAAAGCTGTG 
GAGAAGATGC CCAGAGAGCT GTCCCGGATT GAAGAGGAGA AAGAAGATGA GGAGGAGGAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGACTG AGGTGCTGCT GGATAGCTGT 
GTGGTGTCGC AGGTGGGCGT GGGCCAGAGT GAAGAAGACG GGACCCGGCC CCAGAGCACT 
TCAGATCAGA AGCTGTGGGA GGAAGTTGGG GAGGAGGCCA AGAAGGAGGC TGAAGAGAAG 
GCCAAGGAGG AGGCCGAGGA GGTGGCTGAA GAGGAGGCTG AAAAGGAGCC CCAGGACTGG 
GCGGAGACCA AGGAGGAGCC TGAGGCTGAG GCCGAGGCTG CCAGTTCAGG AGTGCCTGCC 
ACGAAACAGC ACCCAGAAGT GCAGGTGGAA GATACTGATG CTGATAGCTG CCCCCTCATG 
GCAGAAGAGA ATCCACCCTC AACCGTGTTG CCGCCACCGT CTCCTGCCAA ATCAGACACC 
CTTATAGTCC CAAGCTCAGC CTCGGGGACA CACAGGAAGA AGCTGCCCTC TGAGGATGAT 
GAGGCTGAAG AGCTCAAGGC GTTGTCACCA GCAGAGTCCC CAGTGGTTGC CTGGTCTGAC 
CCCACCACCC CGAAGGACAC TGATGGCCAG GACCGTGCGG CCTCCACGGC CAGCACAAAT 
AGCGCCATCA TCAACGACCG GCTCCAGGAG CTGGTGAAGC TCTTCAAGGA GCGGACAGAG 
AAAGTGAAGG AGAAACTCAT TGACCCTGAC GTCACCTCTG ATGAGGAGAG CCCCAAGCCC 
TCCCCAGCCA AGAAAGCCCC AGAGCCAGCT CCAGACACAA AGCCCGCTGA AGCCGAGCCA 
GTGGAAGAGG AGCACTATTG CGACATGCTC TGCTGCAAGT TCAAACACCG CCCCTGGAAG 
AAGTACCAGT TTCCCCAGAG CATTGACCCG CTGACCAACC TGATGTATGT CCTATGGCTG 
TTCTTCGTGG TGATGGCCTG GAATTGGAAC TGTTGGCTGA TTCCCGTGCG CTGGGCCTTC 
CCCTACCAGA CCCCGGACAA CATCCACCAC TGGCTGCTGA TGGATTACCT ATGCGACCTC 
ATCTACTTCC TGGACATCAC CGTGTTCCAG ACACGCCTGC AGTTTGTCAG AGGCGGGGAC 
ATCATTACGG ACAAAAAGGA CATGCGAAAT AACTACCTGA AGTCTCGCCG CTTCAAGATG 
GACCTGCTCA GCCTCCTGCC CTTGGATTTT CTCTATTTGA AAGTCGGTGT GAACCCCCTC 
CTCCGCCTGC CCCGCTGTTT AAAGTACATG GCCTTCTTCG AGTTTAACAG CCGCCTGGAA 
TCCATCCTCA GCAAAGCCTA CGTGTACAGG GTCATCAGGA CCACAGCCTA CCTTCTCTAC 
AGCCTGCATT TGAATTCCTG TCTTTATTAC TGGGCATCGG CCTATCAGGG CCTCGGCTCC 
ACTCACTGGG TTTACGATGG CGTGGGAAAC AGTTATATTC GCTGTTACTA CTTTGCTGTG 
AAGACCCTCA TCACCATCGG GGGGCTGCCT GACCCCAAGA CACTCTTTGA AATTGTCTTC 
CAGCTGCTGA ATTATTTCAC GGGCGTCTTT GCTTTCTCTG TGATGATCGG ACAGATGAGA 
GATGTGGTAG GGGCCGCCAC CGCCGGACAG ACCTACTACC GCAGCTGCAT GGACAGCACG 
GTGAAGTACA TGAATTTCTA CAAGATCCCC AAGTCCGTGC AGAACCGCGT CAAGACCTGG 
TACGAGTACA CCTGGCACTC GCAAGGCATG CTGGATGAGT CAGAGCTGAT GGTGCAGCTT 
CCAGACAAGA TGCGGCTGGA CCTCGCCATC GACGTGAACT ACAACATCGT TAGCAAAGTC 
GCACTCTTTC AGGGCTGTGA CCGGCAGATG ATCTTTGACA TGCTGAAGAG GCTTCGCTCT 
GTTGTCTACC TGCCCAACGA CTATGTGTGC AAGAAGGGGG AGATCGGCCG TGAGATGTAC 
ATCATCCAGG CAGGGCAAGT GCAGGTCTTG GGCGGCCCTG ATGGGAAATC TGTGCTGGTG 
ACGCTGAAAG CTGGATCTGT GTTTGGAGAA ATAAGCTTGC TGGCTGTTGG GGGCGGGAAC 
CGGCGCACGG CCAACGTGGT GGCGCACGGG TTTACCAACC TCTTCATCCT GGATAAGAAG 
GACCTGAATG AGATTTTGGT GCATTATCCT GAGTCTCAGA AGTTACTCCG GAAGAAAGCC 
AGGCGCATGC TGAGAAGCAA CAATAAGCCC AAGGAGGAGA AGAGCGTGCT GATCCTTCCA 
CCCCGGGCGG GCACCCCAAA GCTCTTCAAC GCTGCCCTCG CTATGACAGG AAAGATGGGT 
GGCAAGGGGG CAAAAGGCGG CAAACTTGCT CACCTCCGGG CCCGGCTCAA AGAACTGGCC 
GCGCTGGAGG CGGCTGCAAA GCAGCAAGAG TTGGTGGAAC AGGCCAAGAG CTCGCAAGAC 
GTCAAGGGAG AGGAAGGCTC CGCCGCCCCA GACCAGCACA CGCACCCAAA GGAGGCCGCC 
ACCGACCCAC CCGCGCCCCG GACGCCCCCC GAGCCCCCGG GGTCTCCACC GAGCTCTCCA 
CCGCCTGCCT CCCTTGGGAG GCCGGAGGGA GAGGAGGAGG GGCCGGCCGA GCCCGAAGAG 
CACTCGGTGA GGATCTGCAT GAGCCCGGGC CCGGAGCCGG GAGAGCAGAT CCTGTCGGTG 
AAGATGCCGG AGGAAAGGGA GGAGAAGGCG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB1 ( NM_001297.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288.3 Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 1, mRNA.

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CloneID	OHu18553
Clone ID Related Accession (Same CDS sequence)	NM_001297.4 , NM_001297.5
Accession Version	NM_001297.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010543.9, U18945.1, U58837.1 and BM678849.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001297.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U58837.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000251102.13/ ENSP00000251102.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCATCAGCA TCCTTCCTGG AGGACAAGTG 
GAGCCTGACC TTGTCCTAGA GGAGGTTGAA CCGCCCTGGG AGGATGCCCA CCAGGATGTC 
AGTACCAGCC CACAGGGTAC AGAGGTGGTT CCAGCTTATG AAGAAGAGAA CAAAGCTGTG 
GAGAAGATGC CCAGAGAGCT GTCCCGGATT GAAGAGGAGA AAGAAGATGA GGAGGAGGAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGACTG AGGTGCTGCT GGATAGCTGT 
GTGGTGTCGC AGGTGGGCGT GGGCCAGAGT GAAGAAGACG GGACCCGGCC CCAGAGCACT 
TCAGATCAGA AGCTGTGGGA GGAAGTTGGG GAGGAGGCCA AGAAGGAGGC TGAAGAGAAG 
GCCAAGGAGG AGGCCGAGGA GGTGGCTGAA GAGGAGGCTG AAAAGGAGCC CCAGGACTGG 
GCGGAGACCA AGGAGGAGCC TGAGGCTGAG GCCGAGGCTG CCAGTTCAGG AGTGCCTGCC 
ACGAAACAGC ACCCAGAAGT GCAGGTGGAA GATACTGATG CTGATAGCTG CCCCCTCATG 
GCAGAAGAGA ATCCACCCTC AACCGTGTTG CCGCCACCGT CTCCTGCCAA ATCAGACACC 
CTTATAGTCC CAAGCTCAGC CTCGGGGACA CACAGGAAGA AGCTGCCCTC TGAGGATGAT 
GAGGCTGAAG AGCTCAAGGC GTTGTCACCA GCAGAGTCCC CAGTGGTTGC CTGGTCTGAC 
CCCACCACCC CGAAGGACAC TGATGGCCAG GACCGTGCGG CCTCCACGGC CAGCACAAAT 
AGCGCCATCA TCAACGACCG GCTCCAGGAG CTGGTGAAGC TCTTCAAGGA GCGGACAGAG 
AAAGTGAAGG AGAAACTCAT TGACCCTGAC GTCACCTCTG ATGAGGAGAG CCCCAAGCCC 
TCCCCAGCCA AGAAAGCCCC AGAGCCAGCT CCAGACACAA AGCCCGCTGA AGCCGAGCCA 
GTGGAAGAGG AGCACTATTG CGACATGCTC TGCTGCAAGT TCAAACACCG CCCCTGGAAG 
AAGTACCAGT TTCCCCAGAG CATTGACCCG CTGACCAACC TGATGTATGT CCTATGGCTG 
TTCTTCGTGG TGATGGCCTG GAATTGGAAC TGTTGGCTGA TTCCCGTGCG CTGGGCCTTC 
CCCTACCAGA CCCCGGACAA CATCCACCAC TGGCTGCTGA TGGATTACCT ATGCGACCTC 
ATCTACTTCC TGGACATCAC CGTGTTCCAG ACACGCCTGC AGTTTGTCAG AGGCGGGGAC 
ATCATTACGG ACAAAAAGGA CATGCGAAAT AACTACCTGA AGTCTCGCCG CTTCAAGATG 
GACCTGCTCA GCCTCCTGCC CTTGGATTTT CTCTATTTGA AAGTCGGTGT GAACCCCCTC 
CTCCGCCTGC CCCGCTGTTT AAAGTACATG GCCTTCTTCG AGTTTAACAG CCGCCTGGAA 
TCCATCCTCA GCAAAGCCTA CGTGTACAGG GTCATCAGGA CCACAGCCTA CCTTCTCTAC 
AGCCTGCATT TGAATTCCTG TCTTTATTAC TGGGCATCGG CCTATCAGGG CCTCGGCTCC 
ACTCACTGGG TTTACGATGG CGTGGGAAAC AGTTATATTC GCTGTTACTA CTTTGCTGTG 
AAGACCCTCA TCACCATCGG GGGGCTGCCT GACCCCAAGA CACTCTTTGA AATTGTCTTC 
CAGCTGCTGA ATTATTTCAC GGGCGTCTTT GCTTTCTCTG TGATGATCGG ACAGATGAGA 
GATGTGGTAG GGGCCGCCAC CGCCGGACAG ACCTACTACC GCAGCTGCAT GGACAGCACG 
GTGAAGTACA TGAATTTCTA CAAGATCCCC AAGTCCGTGC AGAACCGCGT CAAGACCTGG 
TACGAGTACA CCTGGCACTC GCAAGGCATG CTGGATGAGT CAGAGCTGAT GGTGCAGCTT 
CCAGACAAGA TGCGGCTGGA CCTCGCCATC GACGTGAACT ACAACATCGT TAGCAAAGTC 
GCACTCTTTC AGGGCTGTGA CCGGCAGATG ATCTTTGACA TGCTGAAGAG GCTTCGCTCT 
GTTGTCTACC TGCCCAACGA CTATGTGTGC AAGAAGGGGG AGATCGGCCG TGAGATGTAC 
ATCATCCAGG CAGGGCAAGT GCAGGTCTTG GGCGGCCCTG ATGGGAAATC TGTGCTGGTG 
ACGCTGAAAG CTGGATCTGT GTTTGGAGAA ATAAGCTTGC TGGCTGTTGG GGGCGGGAAC 
CGGCGCACGG CCAACGTGGT GGCGCACGGG TTTACCAACC TCTTCATCCT GGATAAGAAG 
GACCTGAATG AGATTTTGGT GCATTATCCT GAGTCTCAGA AGTTACTCCG GAAGAAAGCC 
AGGCGCATGC TGAGAAGCAA CAATAAGCCC AAGGAGGAGA AGAGCGTGCT GATCCTTCCA 
CCCCGGGCGG GCACCCCAAA GCTCTTCAAC GCTGCCCTCG CTATGACAGG AAAGATGGGT 
GGCAAGGGGG CAAAAGGCGG CAAACTTGCT CACCTCCGGG CCCGGCTCAA AGAACTGGCC 
GCGCTGGAGG CGGCTGCAAA GCAGCAAGAG TTGGTGGAAC AGGCCAAGAG CTCGCAAGAC 
GTCAAGGGAG AGGAAGGCTC CGCCGCCCCA GACCAGCACA CGCACCCAAA GGAGGCCGCC 
ACCGACCCAC CCGCGCCCCG GACGCCCCCC GAGCCCCCGG GGTCTCCACC GAGCTCTCCA 
CCGCCTGCCT CCCTTGGGAG GCCGGAGGGA GAGGAGGAGG GGCCGGCCGA GCCCGAAGAG 
CACTCGGTGA GGATCTGCAT GAGCCCGGGC CCGGAGCCGG GAGAGCAGAT CCTGTCGGTG 
AAGATGCCGG AGGAAAGGGA GGAGAAGGCG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288.3
CDS	78..3833
Translation	MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPSPQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCTDEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATGAASDPAPPGRPQEMGPKLQARETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQEPDSPGICDVQTISILPGGQVEPDLVLEEVEPPWEDAHQDVSTSPQGTEVVPAYEEENKAVEKMPRELSRIEEEKEDEEEEEEEEEEEEEEEVTEVLLDSCVVSQVGVGQSEEDGTRPQSTSDQKLWEEVGEEAKKEAEEKAKEEAEEVAEEEAEKEPQDWAETKEEPEAEAEAASSGVPATKQHPEVQVEDTDADSCPLMAEENPPSTVLPPPSPAKSDTLIVPSSASGTHRKKLPSEDDEAEELKALSPAESPVVAWSDPTTPKDTDGQDRAASTASTNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPDTKPAEAEPVEEEHYCDMLCCKFKHRPWKKYQFPQSIDPLTNLMYVLWLFFVVMAWNWNCWLIPVRWAFPYQTPDNIHHWLLMDYLCDLIYFLDITVFQTRLQFVRGGDIITDKKDMRNNYLKSRRFKMDLLSLLPLDFLYLKVGVNPLLRLPRCLKYMAFFEFNSRLESILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAYQGLGSTHWVYDGVGNSYIRCYYFAVKTLITIGGLPDPKTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPKSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYNIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKSVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRSNNKPKEEKSVLILPPRAGTPKLFNAALAMTGKMGGKGAKGGKLAHLRARLKELAALEAAAKQQELVEQAKSSQDVKGEEGSAAPDQHTHPKEAATDPPAPRTPPEPPGSPPSSPPPASLGRPEGEEEGPAEPEEHSVRICMSPGPEPGEQILSVKMPEEREEKAE

Target ORF information:

RefSeq Version	NM_001297.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297.5

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGGTGCT GCCTCAGACC CAGCGCCTCC AGGACGCCCC 
CAGGAAATGG GGCCCAAGCT GCAGGCCCGG GAGACCCCCT CCCTGCCCAC ACCCATCCCC 
CTGCAGCCCA AGGAGGAACC CAAGGAGGCA CCAGCTCCAG AGCCCCAGCC CGGCTCCCAG 
GCCCAGACCT CCTCCCTGCC ACCAACCAGG GACCCTGCCA GGCTGGTGGC ATGGGTCCTG 
CACAGGCTGG AGATGGCCTT GCCGCAGCCA GTGCTACATG GGAAAATAGG GGAACAGGAG 
CCTGACTCCC CTGGGATATG TGATGTGCAG ACCATCAGCA TCCTTCCTGG AGGACAAGTG 
GAGCCTGACC TTGTCCTAGA GGAGGTTGAA CCGCCCTGGG AGGATGCCCA CCAGGATGTC 
AGTACCAGCC CACAGGGTAC AGAGGTGGTT CCAGCTTATG AAGAAGAGAA CAAAGCTGTG 
GAGAAGATGC CCAGAGAGCT GTCCCGGATT GAAGAGGAGA AAGAAGATGA GGAGGAGGAA 
GAGGAAGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGACTG AGGTGCTGCT GGATAGCTGT 
GTGGTGTCGC AGGTGGGCGT GGGCCAGAGT GAAGAAGACG GGACCCGGCC CCAGAGCACT 
TCAGATCAGA AGCTGTGGGA GGAAGTTGGG GAGGAGGCCA AGAAGGAGGC TGAAGAGAAG 
GCCAAGGAGG AGGCCGAGGA GGTGGCTGAA GAGGAGGCTG AAAAGGAGCC CCAGGACTGG 
GCGGAGACCA AGGAGGAGCC TGAGGCTGAG GCCGAGGCTG CCAGTTCAGG AGTGCCTGCC 
ACGAAACAGC ACCCAGAAGT GCAGGTGGAA GATACTGATG CTGATAGCTG CCCCCTCATG 
GCAGAAGAGA ATCCACCCTC AACCGTGTTG CCGCCACCGT CTCCTGCCAA ATCAGACACC 
CTTATAGTCC CAAGCTCAGC CTCGGGGACA CACAGGAAGA AGCTGCCCTC TGAGGATGAT 
GAGGCTGAAG AGCTCAAGGC GTTGTCACCA GCAGAGTCCC CAGTGGTTGC CTGGTCTGAC 
CCCACCACCC CGAAGGACAC TGATGGCCAG GACCGTGCGG CCTCCACGGC CAGCACAAAT 
AGCGCCATCA TCAACGACCG GCTCCAGGAG CTGGTGAAGC TCTTCAAGGA GCGGACAGAG 
AAAGTGAAGG AGAAACTCAT TGACCCTGAC GTCACCTCTG ATGAGGAGAG CCCCAAGCCC 
TCCCCAGCCA AGAAAGCCCC AGAGCCAGCT CCAGACACAA AGCCCGCTGA AGCCGAGCCA 
GTGGAAGAGG AGCACTATTG CGACATGCTC TGCTGCAAGT TCAAACACCG CCCCTGGAAG 
AAGTACCAGT TTCCCCAGAG CATTGACCCG CTGACCAACC TGATGTATGT CCTATGGCTG 
TTCTTCGTGG TGATGGCCTG GAATTGGAAC TGTTGGCTGA TTCCCGTGCG CTGGGCCTTC 
CCCTACCAGA CCCCGGACAA CATCCACCAC TGGCTGCTGA TGGATTACCT ATGCGACCTC 
ATCTACTTCC TGGACATCAC CGTGTTCCAG ACACGCCTGC AGTTTGTCAG AGGCGGGGAC 
ATCATTACGG ACAAAAAGGA CATGCGAAAT AACTACCTGA AGTCTCGCCG CTTCAAGATG 
GACCTGCTCA GCCTCCTGCC CTTGGATTTT CTCTATTTGA AAGTCGGTGT GAACCCCCTC 
CTCCGCCTGC CCCGCTGTTT AAAGTACATG GCCTTCTTCG AGTTTAACAG CCGCCTGGAA 
TCCATCCTCA GCAAAGCCTA CGTGTACAGG GTCATCAGGA CCACAGCCTA CCTTCTCTAC 
AGCCTGCATT TGAATTCCTG TCTTTATTAC TGGGCATCGG CCTATCAGGG CCTCGGCTCC 
ACTCACTGGG TTTACGATGG CGTGGGAAAC AGTTATATTC GCTGTTACTA CTTTGCTGTG 
AAGACCCTCA TCACCATCGG GGGGCTGCCT GACCCCAAGA CACTCTTTGA AATTGTCTTC 
CAGCTGCTGA ATTATTTCAC GGGCGTCTTT GCTTTCTCTG TGATGATCGG ACAGATGAGA 
GATGTGGTAG GGGCCGCCAC CGCCGGACAG ACCTACTACC GCAGCTGCAT GGACAGCACG 
GTGAAGTACA TGAATTTCTA CAAGATCCCC AAGTCCGTGC AGAACCGCGT CAAGACCTGG 
TACGAGTACA CCTGGCACTC GCAAGGCATG CTGGATGAGT CAGAGCTGAT GGTGCAGCTT 
CCAGACAAGA TGCGGCTGGA CCTCGCCATC GACGTGAACT ACAACATCGT TAGCAAAGTC 
GCACTCTTTC AGGGCTGTGA CCGGCAGATG ATCTTTGACA TGCTGAAGAG GCTTCGCTCT 
GTTGTCTACC TGCCCAACGA CTATGTGTGC AAGAAGGGGG AGATCGGCCG TGAGATGTAC 
ATCATCCAGG CAGGGCAAGT GCAGGTCTTG GGCGGCCCTG ATGGGAAATC TGTGCTGGTG 
ACGCTGAAAG CTGGATCTGT GTTTGGAGAA ATAAGCTTGC TGGCTGTTGG GGGCGGGAAC 
CGGCGCACGG CCAACGTGGT GGCGCACGGG TTTACCAACC TCTTCATCCT GGATAAGAAG 
GACCTGAATG AGATTTTGGT GCATTATCCT GAGTCTCAGA AGTTACTCCG GAAGAAAGCC 
AGGCGCATGC TGAGAAGCAA CAATAAGCCC AAGGAGGAGA AGAGCGTGCT GATCCTTCCA 
CCCCGGGCGG GCACCCCAAA GCTCTTCAAC GCTGCCCTCG CTATGACAGG AAAGATGGGT 
GGCAAGGGGG CAAAAGGCGG CAAACTTGCT CACCTCCGGG CCCGGCTCAA AGAACTGGCC 
GCGCTGGAGG CGGCTGCAAA GCAGCAAGAG TTGGTGGAAC AGGCCAAGAG CTCGCAAGAC 
GTCAAGGGAG AGGAAGGCTC CGCCGCCCCA GACCAGCACA CGCACCCAAA GGAGGCCGCC 
ACCGACCCAC CCGCGCCCCG GACGCCCCCC GAGCCCCCGG GGTCTCCACC GAGCTCTCCA 
CCGCCTGCCT CCCTTGGGAG GCCGGAGGGA GAGGAGGAGG GGCCGGCCGA GCCCGAAGAG 
CACTCGGTGA GGATCTGCAT GAGCCCGGGC CCGGAGCCGG GAGAGCAGAT CCTGTCGGTG 
AAGATGCCGG AGGAAAGGGA GGAGAAGGCG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB1 ( NM_001286130.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273059.1 Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21806
Clone ID Related Accession (Same CDS sequence)	NM_001286130.1 , NM_001286130.2
Accession Version	NM_001286130.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U18945.1, AF042498.1, AC010543.9, U58837.1 and BM678849.1. On Oct 30, 2013 this sequence version replaced XM_005255797.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042498.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGCGCCT CCAGGACGCC CCCAGGAAAT GGGGCCCAAG 
CTGCAGGCCC GGGAGACCCC CTCCCTGCCC ACACCCATCC CCCTGCAGCC CAAGGAGGAA 
CCCAAGGAGG CACCAGCTCC AGAGCCCCAG CCCGGCTCCC AGGCCCAGAC CTCCTCCCTG 
CCACCAACCA GGGACCCTGC CAGGCTGGTG GCATGGGTCC TGCACAGGCT GGAGATGGCC 
TTGCCGCAGC CAGTGCTACA TGGGAAAATA GGGGAACAGG AGCCTGACTC CCCTGGGATA 
TGTGATGTGC AGACCATCAG CATCCTTCCT GGAGGACAAG TGGAGCCTGA CCTTGTCCTA 
GAGGAGGTTG AACCGCCCTG GGAGGATGCC CACCAGGATG TCAGTACCAG CCCACAGGGT 
ACAGAGGTGG TTCCAGCTTA TGAAGAAGAG AACAAAGCTG TGGAGAAGAT GCCCAGAGAG 
CTGTCCCGGA TTGAAGAGGA GAAAGAAGAT GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGAGG AGGAGGTGAC TGAGGTGCTG CTGGATAGCT GTGTGGTGTC GCAGGTGGGC 
GTGGGCCAGA GTGAAGAAGA CGGGACCCGG CCCCAGAGCA CTTCAGATCA GAAGCTGTGG 
GAGGAAGTTG GGGAGGAGGC CAAGAAGGAG GCTGAAGAGA AGGCCAAGGA GGAGGCCGAG 
GAGGTGGCTG AAGAGGAGGC TGAAAAGGAG CCCCAGGACT GGGCGGAGAC CAAGGAGGAG 
CCTGAGGCTG AGGCCGAGGC TGCCAGTTCA GGAGTGCCTG CCACGAAACA GCACCCAGAA 
GTGCAGGTGG AAGATACTGA TGCTGATAGC TGCCCCCTCA TGGCAGAAGA GAATCCACCC 
TCAACCGTGT TGCCGCCACC GTCTCCTGCC AAATCAGACA CCCTTATAGT CCCAAGCTCA 
GCCTCGGGGA CACACAGGAA GAAGCTGCCC TCTGAGGATG ATGAGGCTGA AGAGCTCAAG 
GCGTTGTCAC CAGCAGAGTC CCCAGTGGTT GCCTGGTCTG ACCCCACCAC CCCGAAGGAC 
ACTGATGGCC AGGACCGTGC GGCCTCCACG GCCAGCACAA ATAGCGCCAT CATCAACGAC 
CGGCTCCAGG AGCTGGTGAA GCTCTTCAAG GAGCGGACAG AGAAAGTGAA GGAGAAACTC 
ATTGACCCTG ACGTCACCTC TGATGAGGAG AGCCCCAAGC CCTCCCCAGC CAAGAAAGCC 
CCAGAGCCAG CTCCAGACAC AAAGCCCGCT GAAGCCGAGC CAGTGGAAGA GGAGCACTAT 
TGCGACATGC TCTGCTGCAA GTTCAAACAC CGCCCCTGGA AGAAGTACCA GTTTCCCCAG 
AGCATTGACC CGCTGACCAA CCTGATGTAT GTCCTATGGC TGTTCTTCGT GGTGATGGCC 
TGGAATTGGA ACTGTTGGCT GATTCCCGTG CGCTGGGCCT TCCCCTACCA GACCCCGGAC 
AACATCCACC ACTGGCTGCT GATGGATTAC CTATGCGACC TCATCTACTT CCTGGACATC 
ACCGTGTTCC AGACACGCCT GCAGTTTGTC AGAGGCGGGG ACATCATTAC GGACAAAAAG 
GACATGCGAA ATAACTACCT GAAGTCTCGC CGCTTCAAGA TGGACCTGCT CAGCCTCCTG 
CCCTTGGATT TTCTCTATTT GAAAGTCGGT GTGAACCCCC TCCTCCGCCT GCCCCGCTGT 
TTAAAGTACA TGGCCTTCTT CGAGTTTAAC AGCCGCCTGG AATCCATCCT CAGCAAAGCC 
TACGTGTACA GGGTCATCAG GACCACAGCC TACCTTCTCT ACAGCCTGCA TTTGAATTCC 
TGTCTTTATT ACTGGGCATC GGCCTATCAG GGCCTCGGCT CCACTCACTG GGTTTACGAT 
GGCGTGGGAA ACAGTTATAT TCGCTGTTAC TACTTTGCTG TGAAGACCCT CATCACCATC 
GGGGGGCTGC CTGACCCCAA GACACTCTTT GAAATTGTCT TCCAGCTGCT GAATTATTTC 
ACGGGCGTCT TTGCTTTCTC TGTGATGATC GGACAGATGA GAGATGTGGT AGGGGCCGCC 
ACCGCCGGAC AGACCTACTA CCGCAGCTGC ATGGACAGCA CGGTGAAGTA CATGAATTTC 
TACAAGATCC CCAAGTCCGT GCAGAACCGC GTCAAGACCT GGTACGAGTA CACCTGGCAC 
TCGCAAGGCA TGCTGGATGA GTCAGAGCTG ATGGTGCAGC TTCCAGACAA GATGCGGCTG 
GACCTCGCCA TCGACGTGAA CTACAACATC GTTAGCAAAG TCGCACTCTT TCAGGGCTGT 
GACCGGCAGA TGATCTTTGA CATGCTGAAG AGGCTTCGCT CTGTTGTCTA CCTGCCCAAC 
GACTATGTGT GCAAGAAGGG GGAGATCGGC CGTGAGATGT ACATCATCCA GGCAGGGCAA 
GTGCAGGTCT TGGGCGGCCC TGATGGGAAA TCTGTGCTGG TGACGCTGAA AGCTGGATCT 
GTGTTTGGAG AAATAAGCTT GCTGGCTGTT GGGGGCGGGA ACCGGCGCAC GGCCAACGTG 
GTGGCGCACG GGTTTACCAA CCTCTTCATC CTGGATAAGA AGGACCTGAA TGAGATTTTG 
GTGCATTATC CTGAGTCTCA GAAGTTACTC CGGAAGAAAG CCAGGCGCAT GCTGAGAAGC 
AACAATAAGC CCAAGGAGGA GAAGAGCGTG CTGATCCTTC CACCCCGGGC GGGCACCCCA 
AAGCTCTTCA ACGCTGCCCT CGCTATGACA GGAAAGATGG GTGGCAAGGG GGCAAAAGGC 
GGCAAACTTG CTCACCTCCG GGCCCGGCTC AAAGAACTGG CCGCGCTGGA GGCGGCTGCA 
AAGCAGCAAG AGTTGGTGGA ACAGGCCAAG AGCTCGCAAG ACGTCAAGGG AGAGGAAGGC 
TCCGCCGCCC CAGACCAGCA CACGCACCCA AAGGAGGCCG CCACCGACCC ACCCGCGCCC 
CGGACGCCCC CCGAGCCCCC GGGGTCTCCA CCGAGCTCTC CACCGCCTGC CTCCCTTGGG 
AGGCCGGAGG GAGAGGAGGA GGGGCCGGCC GAGCCCGAAG AGCACTCGGT GAGGATCTGC 
ATGAGCCCGG GCCCGGAGCC GGGAGAGCAG ATCCTGTCGG TGAAGATGCC GGAGGAAAGG 
GAGGAGAAGG CGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273059.1
CDS	66..3803
Translation	MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPSPQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCTDEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATAPPGRPQEMGPKLQARETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQEPDSPGICDVQTISILPGGQVEPDLVLEEVEPPWEDAHQDVSTSPQGTEVVPAYEEENKAVEKMPRELSRIEEEKEDEEEEEEEEEEEEEEEVTEVLLDSCVVSQVGVGQSEEDGTRPQSTSDQKLWEEVGEEAKKEAEEKAKEEAEEVAEEEAEKEPQDWAETKEEPEAEAEAASSGVPATKQHPEVQVEDTDADSCPLMAEENPPSTVLPPPSPAKSDTLIVPSSASGTHRKKLPSEDDEAEELKALSPAESPVVAWSDPTTPKDTDGQDRAASTASTNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPDTKPAEAEPVEEEHYCDMLCCKFKHRPWKKYQFPQSIDPLTNLMYVLWLFFVVMAWNWNCWLIPVRWAFPYQTPDNIHHWLLMDYLCDLIYFLDITVFQTRLQFVRGGDIITDKKDMRNNYLKSRRFKMDLLSLLPLDFLYLKVGVNPLLRLPRCLKYMAFFEFNSRLESILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAYQGLGSTHWVYDGVGNSYIRCYYFAVKTLITIGGLPDPKTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPKSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYNIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKSVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRSNNKPKEEKSVLILPPRAGTPKLFNAALAMTGKMGGKGAKGGKLAHLRARLKELAALEAAAKQQELVEQAKSSQDVKGEEGSAAPDQHTHPKEAATDPPAPRTPPEPPGSPPSSPPPASLGRPEGEEEGPAEPEEHSVRICMSPGPEPGEQILSVKMPEEREEKAE

Target ORF information:

RefSeq Version	NM_001286130.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel beta 1 (CNGB1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286130.1

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGCGCCT CCAGGACGCC CCCAGGAAAT GGGGCCCAAG 
CTGCAGGCCC GGGAGACCCC CTCCCTGCCC ACACCCATCC CCCTGCAGCC CAAGGAGGAA 
CCCAAGGAGG CACCAGCTCC AGAGCCCCAG CCCGGCTCCC AGGCCCAGAC CTCCTCCCTG 
CCACCAACCA GGGACCCTGC CAGGCTGGTG GCATGGGTCC TGCACAGGCT GGAGATGGCC 
TTGCCGCAGC CAGTGCTACA TGGGAAAATA GGGGAACAGG AGCCTGACTC CCCTGGGATA 
TGTGATGTGC AGACCATCAG CATCCTTCCT GGAGGACAAG TGGAGCCTGA CCTTGTCCTA 
GAGGAGGTTG AACCGCCCTG GGAGGATGCC CACCAGGATG TCAGTACCAG CCCACAGGGT 
ACAGAGGTGG TTCCAGCTTA TGAAGAAGAG AACAAAGCTG TGGAGAAGAT GCCCAGAGAG 
CTGTCCCGGA TTGAAGAGGA GAAAGAAGAT GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGAGG AGGAGGTGAC TGAGGTGCTG CTGGATAGCT GTGTGGTGTC GCAGGTGGGC 
GTGGGCCAGA GTGAAGAAGA CGGGACCCGG CCCCAGAGCA CTTCAGATCA GAAGCTGTGG 
GAGGAAGTTG GGGAGGAGGC CAAGAAGGAG GCTGAAGAGA AGGCCAAGGA GGAGGCCGAG 
GAGGTGGCTG AAGAGGAGGC TGAAAAGGAG CCCCAGGACT GGGCGGAGAC CAAGGAGGAG 
CCTGAGGCTG AGGCCGAGGC TGCCAGTTCA GGAGTGCCTG CCACGAAACA GCACCCAGAA 
GTGCAGGTGG AAGATACTGA TGCTGATAGC TGCCCCCTCA TGGCAGAAGA GAATCCACCC 
TCAACCGTGT TGCCGCCACC GTCTCCTGCC AAATCAGACA CCCTTATAGT CCCAAGCTCA 
GCCTCGGGGA CACACAGGAA GAAGCTGCCC TCTGAGGATG ATGAGGCTGA AGAGCTCAAG 
GCGTTGTCAC CAGCAGAGTC CCCAGTGGTT GCCTGGTCTG ACCCCACCAC CCCGAAGGAC 
ACTGATGGCC AGGACCGTGC GGCCTCCACG GCCAGCACAA ATAGCGCCAT CATCAACGAC 
CGGCTCCAGG AGCTGGTGAA GCTCTTCAAG GAGCGGACAG AGAAAGTGAA GGAGAAACTC 
ATTGACCCTG ACGTCACCTC TGATGAGGAG AGCCCCAAGC CCTCCCCAGC CAAGAAAGCC 
CCAGAGCCAG CTCCAGACAC AAAGCCCGCT GAAGCCGAGC CAGTGGAAGA GGAGCACTAT 
TGCGACATGC TCTGCTGCAA GTTCAAACAC CGCCCCTGGA AGAAGTACCA GTTTCCCCAG 
AGCATTGACC CGCTGACCAA CCTGATGTAT GTCCTATGGC TGTTCTTCGT GGTGATGGCC 
TGGAATTGGA ACTGTTGGCT GATTCCCGTG CGCTGGGCCT TCCCCTACCA GACCCCGGAC 
AACATCCACC ACTGGCTGCT GATGGATTAC CTATGCGACC TCATCTACTT CCTGGACATC 
ACCGTGTTCC AGACACGCCT GCAGTTTGTC AGAGGCGGGG ACATCATTAC GGACAAAAAG 
GACATGCGAA ATAACTACCT GAAGTCTCGC CGCTTCAAGA TGGACCTGCT CAGCCTCCTG 
CCCTTGGATT TTCTCTATTT GAAAGTCGGT GTGAACCCCC TCCTCCGCCT GCCCCGCTGT 
TTAAAGTACA TGGCCTTCTT CGAGTTTAAC AGCCGCCTGG AATCCATCCT CAGCAAAGCC 
TACGTGTACA GGGTCATCAG GACCACAGCC TACCTTCTCT ACAGCCTGCA TTTGAATTCC 
TGTCTTTATT ACTGGGCATC GGCCTATCAG GGCCTCGGCT CCACTCACTG GGTTTACGAT 
GGCGTGGGAA ACAGTTATAT TCGCTGTTAC TACTTTGCTG TGAAGACCCT CATCACCATC 
GGGGGGCTGC CTGACCCCAA GACACTCTTT GAAATTGTCT TCCAGCTGCT GAATTATTTC 
ACGGGCGTCT TTGCTTTCTC TGTGATGATC GGACAGATGA GAGATGTGGT AGGGGCCGCC 
ACCGCCGGAC AGACCTACTA CCGCAGCTGC ATGGACAGCA CGGTGAAGTA CATGAATTTC 
TACAAGATCC CCAAGTCCGT GCAGAACCGC GTCAAGACCT GGTACGAGTA CACCTGGCAC 
TCGCAAGGCA TGCTGGATGA GTCAGAGCTG ATGGTGCAGC TTCCAGACAA GATGCGGCTG 
GACCTCGCCA TCGACGTGAA CTACAACATC GTTAGCAAAG TCGCACTCTT TCAGGGCTGT 
GACCGGCAGA TGATCTTTGA CATGCTGAAG AGGCTTCGCT CTGTTGTCTA CCTGCCCAAC 
GACTATGTGT GCAAGAAGGG GGAGATCGGC CGTGAGATGT ACATCATCCA GGCAGGGCAA 
GTGCAGGTCT TGGGCGGCCC TGATGGGAAA TCTGTGCTGG TGACGCTGAA AGCTGGATCT 
GTGTTTGGAG AAATAAGCTT GCTGGCTGTT GGGGGCGGGA ACCGGCGCAC GGCCAACGTG 
GTGGCGCACG GGTTTACCAA CCTCTTCATC CTGGATAAGA AGGACCTGAA TGAGATTTTG 
GTGCATTATC CTGAGTCTCA GAAGTTACTC CGGAAGAAAG CCAGGCGCAT GCTGAGAAGC 
AACAATAAGC CCAAGGAGGA GAAGAGCGTG CTGATCCTTC CACCCCGGGC GGGCACCCCA 
AAGCTCTTCA ACGCTGCCCT CGCTATGACA GGAAAGATGG GTGGCAAGGG GGCAAAAGGC 
GGCAAACTTG CTCACCTCCG GGCCCGGCTC AAAGAACTGG CCGCGCTGGA GGCGGCTGCA 
AAGCAGCAAG AGTTGGTGGA ACAGGCCAAG AGCTCGCAAG ACGTCAAGGG AGAGGAAGGC 
TCCGCCGCCC CAGACCAGCA CACGCACCCA AAGGAGGCCG CCACCGACCC ACCCGCGCCC 
CGGACGCCCC CCGAGCCCCC GGGGTCTCCA CCGAGCTCTC CACCGCCTGC CTCCCTTGGG 
AGGCCGGAGG GAGAGGAGGA GGGGCCGGCC GAGCCCGAAG AGCACTCGGT GAGGATCTGC 
ATGAGCCCGG GCCCGGAGCC GGGAGAGCAG ATCCTGTCGG TGAAGATGCC GGAGGAAAGG 
GAGGAGAAGG CGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB1 ( NM_001286130.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273059.1 Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu21806
Clone ID Related Accession (Same CDS sequence)	NM_001286130.1 , NM_001286130.2
Accession Version	NM_001286130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010543.9, U18945.1, AF042498.1, U58837.1 and BM678849.1. On May 31, 2019 this sequence version replaced NM_001286130.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF042498.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGCGCCT CCAGGACGCC CCCAGGAAAT GGGGCCCAAG 
CTGCAGGCCC GGGAGACCCC CTCCCTGCCC ACACCCATCC CCCTGCAGCC CAAGGAGGAA 
CCCAAGGAGG CACCAGCTCC AGAGCCCCAG CCCGGCTCCC AGGCCCAGAC CTCCTCCCTG 
CCACCAACCA GGGACCCTGC CAGGCTGGTG GCATGGGTCC TGCACAGGCT GGAGATGGCC 
TTGCCGCAGC CAGTGCTACA TGGGAAAATA GGGGAACAGG AGCCTGACTC CCCTGGGATA 
TGTGATGTGC AGACCATCAG CATCCTTCCT GGAGGACAAG TGGAGCCTGA CCTTGTCCTA 
GAGGAGGTTG AACCGCCCTG GGAGGATGCC CACCAGGATG TCAGTACCAG CCCACAGGGT 
ACAGAGGTGG TTCCAGCTTA TGAAGAAGAG AACAAAGCTG TGGAGAAGAT GCCCAGAGAG 
CTGTCCCGGA TTGAAGAGGA GAAAGAAGAT GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGAGG AGGAGGTGAC TGAGGTGCTG CTGGATAGCT GTGTGGTGTC GCAGGTGGGC 
GTGGGCCAGA GTGAAGAAGA CGGGACCCGG CCCCAGAGCA CTTCAGATCA GAAGCTGTGG 
GAGGAAGTTG GGGAGGAGGC CAAGAAGGAG GCTGAAGAGA AGGCCAAGGA GGAGGCCGAG 
GAGGTGGCTG AAGAGGAGGC TGAAAAGGAG CCCCAGGACT GGGCGGAGAC CAAGGAGGAG 
CCTGAGGCTG AGGCCGAGGC TGCCAGTTCA GGAGTGCCTG CCACGAAACA GCACCCAGAA 
GTGCAGGTGG AAGATACTGA TGCTGATAGC TGCCCCCTCA TGGCAGAAGA GAATCCACCC 
TCAACCGTGT TGCCGCCACC GTCTCCTGCC AAATCAGACA CCCTTATAGT CCCAAGCTCA 
GCCTCGGGGA CACACAGGAA GAAGCTGCCC TCTGAGGATG ATGAGGCTGA AGAGCTCAAG 
GCGTTGTCAC CAGCAGAGTC CCCAGTGGTT GCCTGGTCTG ACCCCACCAC CCCGAAGGAC 
ACTGATGGCC AGGACCGTGC GGCCTCCACG GCCAGCACAA ATAGCGCCAT CATCAACGAC 
CGGCTCCAGG AGCTGGTGAA GCTCTTCAAG GAGCGGACAG AGAAAGTGAA GGAGAAACTC 
ATTGACCCTG ACGTCACCTC TGATGAGGAG AGCCCCAAGC CCTCCCCAGC CAAGAAAGCC 
CCAGAGCCAG CTCCAGACAC AAAGCCCGCT GAAGCCGAGC CAGTGGAAGA GGAGCACTAT 
TGCGACATGC TCTGCTGCAA GTTCAAACAC CGCCCCTGGA AGAAGTACCA GTTTCCCCAG 
AGCATTGACC CGCTGACCAA CCTGATGTAT GTCCTATGGC TGTTCTTCGT GGTGATGGCC 
TGGAATTGGA ACTGTTGGCT GATTCCCGTG CGCTGGGCCT TCCCCTACCA GACCCCGGAC 
AACATCCACC ACTGGCTGCT GATGGATTAC CTATGCGACC TCATCTACTT CCTGGACATC 
ACCGTGTTCC AGACACGCCT GCAGTTTGTC AGAGGCGGGG ACATCATTAC GGACAAAAAG 
GACATGCGAA ATAACTACCT GAAGTCTCGC CGCTTCAAGA TGGACCTGCT CAGCCTCCTG 
CCCTTGGATT TTCTCTATTT GAAAGTCGGT GTGAACCCCC TCCTCCGCCT GCCCCGCTGT 
TTAAAGTACA TGGCCTTCTT CGAGTTTAAC AGCCGCCTGG AATCCATCCT CAGCAAAGCC 
TACGTGTACA GGGTCATCAG GACCACAGCC TACCTTCTCT ACAGCCTGCA TTTGAATTCC 
TGTCTTTATT ACTGGGCATC GGCCTATCAG GGCCTCGGCT CCACTCACTG GGTTTACGAT 
GGCGTGGGAA ACAGTTATAT TCGCTGTTAC TACTTTGCTG TGAAGACCCT CATCACCATC 
GGGGGGCTGC CTGACCCCAA GACACTCTTT GAAATTGTCT TCCAGCTGCT GAATTATTTC 
ACGGGCGTCT TTGCTTTCTC TGTGATGATC GGACAGATGA GAGATGTGGT AGGGGCCGCC 
ACCGCCGGAC AGACCTACTA CCGCAGCTGC ATGGACAGCA CGGTGAAGTA CATGAATTTC 
TACAAGATCC CCAAGTCCGT GCAGAACCGC GTCAAGACCT GGTACGAGTA CACCTGGCAC 
TCGCAAGGCA TGCTGGATGA GTCAGAGCTG ATGGTGCAGC TTCCAGACAA GATGCGGCTG 
GACCTCGCCA TCGACGTGAA CTACAACATC GTTAGCAAAG TCGCACTCTT TCAGGGCTGT 
GACCGGCAGA TGATCTTTGA CATGCTGAAG AGGCTTCGCT CTGTTGTCTA CCTGCCCAAC 
GACTATGTGT GCAAGAAGGG GGAGATCGGC CGTGAGATGT ACATCATCCA GGCAGGGCAA 
GTGCAGGTCT TGGGCGGCCC TGATGGGAAA TCTGTGCTGG TGACGCTGAA AGCTGGATCT 
GTGTTTGGAG AAATAAGCTT GCTGGCTGTT GGGGGCGGGA ACCGGCGCAC GGCCAACGTG 
GTGGCGCACG GGTTTACCAA CCTCTTCATC CTGGATAAGA AGGACCTGAA TGAGATTTTG 
GTGCATTATC CTGAGTCTCA GAAGTTACTC CGGAAGAAAG CCAGGCGCAT GCTGAGAAGC 
AACAATAAGC CCAAGGAGGA GAAGAGCGTG CTGATCCTTC CACCCCGGGC GGGCACCCCA 
AAGCTCTTCA ACGCTGCCCT CGCTATGACA GGAAAGATGG GTGGCAAGGG GGCAAAAGGC 
GGCAAACTTG CTCACCTCCG GGCCCGGCTC AAAGAACTGG CCGCGCTGGA GGCGGCTGCA 
AAGCAGCAAG AGTTGGTGGA ACAGGCCAAG AGCTCGCAAG ACGTCAAGGG AGAGGAAGGC 
TCCGCCGCCC CAGACCAGCA CACGCACCCA AAGGAGGCCG CCACCGACCC ACCCGCGCCC 
CGGACGCCCC CCGAGCCCCC GGGGTCTCCA CCGAGCTCTC CACCGCCTGC CTCCCTTGGG 
AGGCCGGAGG GAGAGGAGGA GGGGCCGGCC GAGCCCGAAG AGCACTCGGT GAGGATCTGC 
ATGAGCCCGG GCCCGGAGCC GGGAGAGCAG ATCCTGTCGG TGAAGATGCC GGAGGAAAGG 
GAGGAGAAGG CGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273059.1
CDS	78..3815
Translation	MLGWVQRVLPQPPGTPRKTKMQEEEEVEPEPEMEAEVEPEPNPEEAETESESMPPEESFKEEEVAVADPSPQETKEAALTSTISLRAQGAEISEMNSPSRRVLTWLMKGVEKVIPQPVHSITEDPAQILGHGSTGDTGCTDEPNEALEAQDTRPGLRLLLWLEQNLERVLPQPPKSSEVWRDEPAVATAPPGRPQEMGPKLQARETPSLPTPIPLQPKEEPKEAPAPEPQPGSQAQTSSLPPTRDPARLVAWVLHRLEMALPQPVLHGKIGEQEPDSPGICDVQTISILPGGQVEPDLVLEEVEPPWEDAHQDVSTSPQGTEVVPAYEEENKAVEKMPRELSRIEEEKEDEEEEEEEEEEEEEEEVTEVLLDSCVVSQVGVGQSEEDGTRPQSTSDQKLWEEVGEEAKKEAEEKAKEEAEEVAEEEAEKEPQDWAETKEEPEAEAEAASSGVPATKQHPEVQVEDTDADSCPLMAEENPPSTVLPPPSPAKSDTLIVPSSASGTHRKKLPSEDDEAEELKALSPAESPVVAWSDPTTPKDTDGQDRAASTASTNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPDTKPAEAEPVEEEHYCDMLCCKFKHRPWKKYQFPQSIDPLTNLMYVLWLFFVVMAWNWNCWLIPVRWAFPYQTPDNIHHWLLMDYLCDLIYFLDITVFQTRLQFVRGGDIITDKKDMRNNYLKSRRFKMDLLSLLPLDFLYLKVGVNPLLRLPRCLKYMAFFEFNSRLESILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAYQGLGSTHWVYDGVGNSYIRCYYFAVKTLITIGGLPDPKTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPKSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYNIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKSVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRSNNKPKEEKSVLILPPRAGTPKLFNAALAMTGKMGGKGAKGGKLAHLRARLKELAALEAAAKQQELVEQAKSSQDVKGEEGSAAPDQHTHPKEAATDPPAPRTPPEPPGSPPSSPPPASLGRPEGEEEGPAEPEEHSVRICMSPGPEPGEQILSVKMPEEREEKAE

Target ORF information:

RefSeq Version	NM_001286130.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286130.2

ORF Insert Sequence:

ATGTTGGGCT GGGTCCAGAG GGTGCTGCCT CAGCCCCCAG GGACCCCTCG GAAGACCAAG 
ATGCAGGAGG AAGAGGAAGT GGAACCAGAG CCAGAGATGG AGGCGGAGGT GGAACCAGAA 
CCGAATCCTG AGGAGGCCGA GACAGAGTCC GAGTCCATGC CCCCCGAAGA GTCATTCAAG 
GAGGAGGAAG TGGCTGTGGC AGACCCAAGC CCTCAGGAGA CCAAGGAGGC TGCCCTTACT 
TCCACCATAT CCCTCCGGGC CCAGGGCGCT GAGATTTCTG AAATGAATAG TCCCAGCCGC 
AGGGTACTGA CCTGGCTCAT GAAGGGCGTA GAGAAGGTGA TCCCGCAGCC TGTTCACAGC 
ATCACGGAGG ACCCGGCTCA GATCCTGGGG CATGGCAGCA CTGGGGACAC AGGGTGCACA 
GATGAACCCA ATGAGGCCCT TGAGGCCCAA GACACTAGGC CTGGGCTGCG GCTGCTTCTG 
TGGCTGGAGC AGAATCTGGA AAGAGTGCTT CCTCAGCCCC CCAAATCCTC TGAGGTCTGG 
AGAGATGAGC CTGCAGTTGC TACAGCGCCT CCAGGACGCC CCCAGGAAAT GGGGCCCAAG 
CTGCAGGCCC GGGAGACCCC CTCCCTGCCC ACACCCATCC CCCTGCAGCC CAAGGAGGAA 
CCCAAGGAGG CACCAGCTCC AGAGCCCCAG CCCGGCTCCC AGGCCCAGAC CTCCTCCCTG 
CCACCAACCA GGGACCCTGC CAGGCTGGTG GCATGGGTCC TGCACAGGCT GGAGATGGCC 
TTGCCGCAGC CAGTGCTACA TGGGAAAATA GGGGAACAGG AGCCTGACTC CCCTGGGATA 
TGTGATGTGC AGACCATCAG CATCCTTCCT GGAGGACAAG TGGAGCCTGA CCTTGTCCTA 
GAGGAGGTTG AACCGCCCTG GGAGGATGCC CACCAGGATG TCAGTACCAG CCCACAGGGT 
ACAGAGGTGG TTCCAGCTTA TGAAGAAGAG AACAAAGCTG TGGAGAAGAT GCCCAGAGAG 
CTGTCCCGGA TTGAAGAGGA GAAAGAAGAT GAGGAGGAGG AAGAGGAAGA GGAGGAGGAG 
GAGGAAGAGG AGGAGGTGAC TGAGGTGCTG CTGGATAGCT GTGTGGTGTC GCAGGTGGGC 
GTGGGCCAGA GTGAAGAAGA CGGGACCCGG CCCCAGAGCA CTTCAGATCA GAAGCTGTGG 
GAGGAAGTTG GGGAGGAGGC CAAGAAGGAG GCTGAAGAGA AGGCCAAGGA GGAGGCCGAG 
GAGGTGGCTG AAGAGGAGGC TGAAAAGGAG CCCCAGGACT GGGCGGAGAC CAAGGAGGAG 
CCTGAGGCTG AGGCCGAGGC TGCCAGTTCA GGAGTGCCTG CCACGAAACA GCACCCAGAA 
GTGCAGGTGG AAGATACTGA TGCTGATAGC TGCCCCCTCA TGGCAGAAGA GAATCCACCC 
TCAACCGTGT TGCCGCCACC GTCTCCTGCC AAATCAGACA CCCTTATAGT CCCAAGCTCA 
GCCTCGGGGA CACACAGGAA GAAGCTGCCC TCTGAGGATG ATGAGGCTGA AGAGCTCAAG 
GCGTTGTCAC CAGCAGAGTC CCCAGTGGTT GCCTGGTCTG ACCCCACCAC CCCGAAGGAC 
ACTGATGGCC AGGACCGTGC GGCCTCCACG GCCAGCACAA ATAGCGCCAT CATCAACGAC 
CGGCTCCAGG AGCTGGTGAA GCTCTTCAAG GAGCGGACAG AGAAAGTGAA GGAGAAACTC 
ATTGACCCTG ACGTCACCTC TGATGAGGAG AGCCCCAAGC CCTCCCCAGC CAAGAAAGCC 
CCAGAGCCAG CTCCAGACAC AAAGCCCGCT GAAGCCGAGC CAGTGGAAGA GGAGCACTAT 
TGCGACATGC TCTGCTGCAA GTTCAAACAC CGCCCCTGGA AGAAGTACCA GTTTCCCCAG 
AGCATTGACC CGCTGACCAA CCTGATGTAT GTCCTATGGC TGTTCTTCGT GGTGATGGCC 
TGGAATTGGA ACTGTTGGCT GATTCCCGTG CGCTGGGCCT TCCCCTACCA GACCCCGGAC 
AACATCCACC ACTGGCTGCT GATGGATTAC CTATGCGACC TCATCTACTT CCTGGACATC 
ACCGTGTTCC AGACACGCCT GCAGTTTGTC AGAGGCGGGG ACATCATTAC GGACAAAAAG 
GACATGCGAA ATAACTACCT GAAGTCTCGC CGCTTCAAGA TGGACCTGCT CAGCCTCCTG 
CCCTTGGATT TTCTCTATTT GAAAGTCGGT GTGAACCCCC TCCTCCGCCT GCCCCGCTGT 
TTAAAGTACA TGGCCTTCTT CGAGTTTAAC AGCCGCCTGG AATCCATCCT CAGCAAAGCC 
TACGTGTACA GGGTCATCAG GACCACAGCC TACCTTCTCT ACAGCCTGCA TTTGAATTCC 
TGTCTTTATT ACTGGGCATC GGCCTATCAG GGCCTCGGCT CCACTCACTG GGTTTACGAT 
GGCGTGGGAA ACAGTTATAT TCGCTGTTAC TACTTTGCTG TGAAGACCCT CATCACCATC 
GGGGGGCTGC CTGACCCCAA GACACTCTTT GAAATTGTCT TCCAGCTGCT GAATTATTTC 
ACGGGCGTCT TTGCTTTCTC TGTGATGATC GGACAGATGA GAGATGTGGT AGGGGCCGCC 
ACCGCCGGAC AGACCTACTA CCGCAGCTGC ATGGACAGCA CGGTGAAGTA CATGAATTTC 
TACAAGATCC CCAAGTCCGT GCAGAACCGC GTCAAGACCT GGTACGAGTA CACCTGGCAC 
TCGCAAGGCA TGCTGGATGA GTCAGAGCTG ATGGTGCAGC TTCCAGACAA GATGCGGCTG 
GACCTCGCCA TCGACGTGAA CTACAACATC GTTAGCAAAG TCGCACTCTT TCAGGGCTGT 
GACCGGCAGA TGATCTTTGA CATGCTGAAG AGGCTTCGCT CTGTTGTCTA CCTGCCCAAC 
GACTATGTGT GCAAGAAGGG GGAGATCGGC CGTGAGATGT ACATCATCCA GGCAGGGCAA 
GTGCAGGTCT TGGGCGGCCC TGATGGGAAA TCTGTGCTGG TGACGCTGAA AGCTGGATCT 
GTGTTTGGAG AAATAAGCTT GCTGGCTGTT GGGGGCGGGA ACCGGCGCAC GGCCAACGTG 
GTGGCGCACG GGTTTACCAA CCTCTTCATC CTGGATAAGA AGGACCTGAA TGAGATTTTG 
GTGCATTATC CTGAGTCTCA GAAGTTACTC CGGAAGAAAG CCAGGCGCAT GCTGAGAAGC 
AACAATAAGC CCAAGGAGGA GAAGAGCGTG CTGATCCTTC CACCCCGGGC GGGCACCCCA 
AAGCTCTTCA ACGCTGCCCT CGCTATGACA GGAAAGATGG GTGGCAAGGG GGCAAAAGGC 
GGCAAACTTG CTCACCTCCG GGCCCGGCTC AAAGAACTGG CCGCGCTGGA GGCGGCTGCA 
AAGCAGCAAG AGTTGGTGGA ACAGGCCAAG AGCTCGCAAG ACGTCAAGGG AGAGGAAGGC 
TCCGCCGCCC CAGACCAGCA CACGCACCCA AAGGAGGCCG CCACCGACCC ACCCGCGCCC 
CGGACGCCCC CCGAGCCCCC GGGGTCTCCA CCGAGCTCTC CACCGCCTGC CTCCCTTGGG 
AGGCCGGAGG GAGAGGAGGA GGGGCCGGCC GAGCCCGAAG AGCACTCGGT GAGGATCTGC 
ATGAGCCCGG GCCCGGAGCC GGGAGAGCAG ATCCTGTCGG TGAAGATGCC GGAGGAAAGG 
GAGGAGAAGG CGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNGB1 ( XM_011522870.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521172.1 Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant X1, mRNA.

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CloneID	OHu56956
Clone ID Related Accession (Same CDS sequence)	XM_011522870.2
Accession Version	XM_011522870.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cyclic nucleotide-gated cation channel beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACAC CATGTCAGAA CATGGAGCTG AATTGCCTAG TCCAGGACCA GATACCTGGC 
CAGGTGGCCT CTGCCTCTGC CCTCCAAAAC CTGGTAGAGC ATCTCCCTAA TGTGCCCAGC 
TACCGCATCC CAATCACCCG CATCCCTGTC CTCACCTCCC GGAGAACCAG CTTGTCCAAC 
TCCAGCTTCG CCAAGGAGAC CAGGAGCTCC ATCCGCCAAC TAGTGCCTGC CACGAAACAG 
CACCCAGAAG TGCAGGTGGA AGATACTGAT GCTGATAGCT GCCCCCTCAT GGCAGAAGAG 
AATCCACCCT CAACCGTGTT GCCGCCACCG TCTCCTGCCA AATCAGACAC CCTTATAGTC 
CCAAGCTCAG CCTCGGGGAC ACACAGGAAG AAGCTGCCCT CTGAGGATGA TGAGGCTGAA 
GAGCTCAAGG CGTTGTCACC AGCAGAGTCC CCAGTGGTTG CCTGGTCTGA CCCCACCACC 
CCGAAGGACA CTGATGGCCA GGACCGTGCG GCCTCCACGG CCAGCACAAA TAGCGCCATC 
ATCAACGACC GGCTCCAGGA GCTGGTGAAG CTCTTCAAGG AGCGGACAGA GAAAGTGAAG 
GAGAAACTCA TTGACCCTGA CGTCACCTCT GATGAGGAGA GCCCCAAGCC CTCCCCAGCC 
AAGAAAGCCC CAGAGCCAGC TCCAGACACA AAGCCCGCTG AAGCCGAGCC AGTGGAAGAG 
GAGCACTATT GCGACATGCT CTGCTGCAAG TTCAAACACC GCCCCTGGAA GAAGTACCAG 
TTTCCCCAGA GCATTGACCC GCTGACCAAC CTGATGTATG TCCTATGGCT GTTCTTCGTG 
GTGATGGCCT GGAATTGGAA CTGTTGGCTG ATTCCCGTGC GCTGGGCCTT CCCCTACCAG 
ACCCCGGACA ACATCCACCA CTGGCTGCTG ATGGATTACC TATGCGACCT CATCTACTTC 
CTGGACATCA CCGTGTTCCA GACACGCCTG CAGTTTGTCA GAGGCGGGGA CATCATTACG 
GACAAAAAGG ACATGCGAAA TAACTACCTG AAGTCTCGCC GCTTCAAGAT GGACCTGCTC 
AGCCTCCTGC CCTTGGATTT TCTCTATTTG AAAGTCGGTG TGAACCCCCT CCTCCGCCTG 
CCCCGCTGTT TAAAGTACAT GGCCTTCTTC GAGTTTAACA GCCGCCTGGA ATCCATCCTC 
AGCAAAGCCT ACGTGTACAG GGTCATCAGG ACCACAGCCT ACCTTCTCTA CAGCCTGCAT 
TTGAATTCCT GTCTTTATTA CTGGGCATCG GCCTATCAGG GCCTCGGCTC CACTCACTGG 
GTTTACGATG GCGTGGGAAA CAGTTATATT CGCTGTTACT ACTTTGCTGT GAAGACCCTC 
ATCACCATCG GGGGGCTGCC TGACCCCAAG ACACTCTTTG AAATTGTCTT CCAGCTGCTG 
AATTATTTCA CGGGCGTCTT TGCTTTCTCT GTGATGATCG GACAGATGAG AGATGTGGTA 
GGGGCCGCCA CCGCCGGACA GACCTACTAC CGCAGCTGCA TGGACAGCAC GGTGAAGTAC 
ATGAATTTCT ACAAGATCCC CAAGTCCGTG CAGAACCGCG TCAAGACCTG GTACGAGTAC 
ACCTGGCACT CGCAAGGCAT GCTGGATGAG TCAGAGCTGA TGGTGCAGCT TCCAGACAAG 
ATGCGGCTGG ACCTCGCCAT CGACGTGAAC TACAACATCG TTAGCAAAGT CGCACTCTTT 
CAGGGCTGTG ACCGGCAGAT GATCTTTGAC ATGCTGAAGA GGCTTCGCTC TGTTGTCTAC 
CTGCCCAACG ACTATGTGTG CAAGAAGGGG GAGATCGGCC GTGAGATGTA CATCATCCAG 
GCAGGGCAAG TGCAGGTCTT GGGCGGCCCT GATGGGAAAT CTGTGCTGGT GACGCTGAAA 
GCTGGATCTG TGTTTGGAGA AATAAGCTTG CTGGCTGTTG GGGGCGGGAA CCGGCGCACG 
GCCAACGTGG TGGCGCACGG GTTTACCAAC CTCTTCATCC TGGATAAGAA GGACCTGAAT 
GAGATTTTGG TGCATTATCC TGAGTCTCAG AAGTTACTCC GGAAGAAAGC CAGGCGCATG 
CTGAGAAGCA ACAATAAGCC CAAGGAGGAG AAGAGCGTGC TGATCCTTCC ACCCCGGGCG 
GGCACCCCAA AGCTCTTCAA CGCTGCCCTC GCTATGACAG GAAAGATGGG TGGCAAGGGG 
GCAAAAGGCG GCAAACTTGC TCACCTCCGG GCCCGGCTCA AAGAACTGGC CGCGCTGGAG 
GCGGCTGCAA AGCAGCAAGA GTTGGTGGAA CAGGCCAAGA GCTCGCAAGA CGTCAAGGGA 
GAGGAAGGCT CCGCCGCCCC AGACCAGCAC ACGCACCCAA AGGAGGCCGC CACCGACCCA 
CCCGCGCCCC GGACGCCCCC CGAGCCCCCG GGGTCTCCAC CGAGCTCTCC ACCGCCTGCC 
TCCCTTGGGA GGCCGGAGGG AGAGGAGGAG GGGCCGGCCG AGCCCGAAGA GCACTCGGTG 
AGGATCTGCA TGAGCCCGGG CCCGGAGCCG GGAGAGCAGA TCCTGTCGGT GAAGATGCCG 
GAGGAAAGGG AGGAGAAGGC GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521172.1
CDS	484..3090
Translation	MATPCQNMELNCLVQDQIPGQVASASALQNLVEHLPNVPSYRIPITRIPVLTSRRTSLSNSSFAKETRSSIRQLVPATKQHPEVQVEDTDADSCPLMAEENPPSTVLPPPSPAKSDTLIVPSSASGTHRKKLPSEDDEAEELKALSPAESPVVAWSDPTTPKDTDGQDRAASTASTNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPDTKPAEAEPVEEEHYCDMLCCKFKHRPWKKYQFPQSIDPLTNLMYVLWLFFVVMAWNWNCWLIPVRWAFPYQTPDNIHHWLLMDYLCDLIYFLDITVFQTRLQFVRGGDIITDKKDMRNNYLKSRRFKMDLLSLLPLDFLYLKVGVNPLLRLPRCLKYMAFFEFNSRLESILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAYQGLGSTHWVYDGVGNSYIRCYYFAVKTLITIGGLPDPKTLFEIVFQLLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPKSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYNIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKSVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRSNNKPKEEKSVLILPPRAGTPKLFNAALAMTGKMGGKGAKGGKLAHLRARLKELAALEAAAKQQELVEQAKSSQDVKGEEGSAAPDQHTHPKEAATDPPAPRTPPEPPGSPPSSPPPASLGRPEGEEEGPAEPEEHSVRICMSPGPEPGEQILSVKMPEEREEKAE

Target ORF information:

RefSeq Version	XM_011522870.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cyclic nucleotide gated channel subunit beta 1 (CNGB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522870.2

ORF Insert Sequence:

ATGGCTACAC CATGTCAGAA CATGGAGCTG AATTGCCTAG TCCAGGACCA GATACCTGGC 
CAGGTGGCCT CTGCCTCTGC CCTCCAAAAC CTGGTAGAGC ATCTCCCTAA TGTGCCCAGC 
TACCGCATCC CAATCACCCG CATCCCTGTC CTCACCTCCC GGAGAACCAG CTTGTCCAAC 
TCCAGCTTCG CCAAGGAGAC CAGGAGCTCC ATCCGCCAAC TAGTGCCTGC CACGAAACAG 
CACCCAGAAG TGCAGGTGGA AGATACTGAT GCTGATAGCT GCCCCCTCAT GGCAGAAGAG 
AATCCACCCT CAACCGTGTT GCCGCCACCG TCTCCTGCCA AATCAGACAC CCTTATAGTC 
CCAAGCTCAG CCTCGGGGAC ACACAGGAAG AAGCTGCCCT CTGAGGATGA TGAGGCTGAA 
GAGCTCAAGG CGTTGTCACC AGCAGAGTCC CCAGTGGTTG CCTGGTCTGA CCCCACCACC 
CCGAAGGACA CTGATGGCCA GGACCGTGCG GCCTCCACGG CCAGCACAAA TAGCGCCATC 
ATCAACGACC GGCTCCAGGA GCTGGTGAAG CTCTTCAAGG AGCGGACAGA GAAAGTGAAG 
GAGAAACTCA TTGACCCTGA CGTCACCTCT GATGAGGAGA GCCCCAAGCC CTCCCCAGCC 
AAGAAAGCCC CAGAGCCAGC TCCAGACACA AAGCCCGCTG AAGCCGAGCC AGTGGAAGAG 
GAGCACTATT GCGACATGCT CTGCTGCAAG TTCAAACACC GCCCCTGGAA GAAGTACCAG 
TTTCCCCAGA GCATTGACCC GCTGACCAAC CTGATGTATG TCCTATGGCT GTTCTTCGTG 
GTGATGGCCT GGAATTGGAA CTGTTGGCTG ATTCCCGTGC GCTGGGCCTT CCCCTACCAG 
ACCCCGGACA ACATCCACCA CTGGCTGCTG ATGGATTACC TATGCGACCT CATCTACTTC 
CTGGACATCA CCGTGTTCCA GACACGCCTG CAGTTTGTCA GAGGCGGGGA CATCATTACG 
GACAAAAAGG ACATGCGAAA TAACTACCTG AAGTCTCGCC GCTTCAAGAT GGACCTGCTC 
AGCCTCCTGC CCTTGGATTT TCTCTATTTG AAAGTCGGTG TGAACCCCCT CCTCCGCCTG 
CCCCGCTGTT TAAAGTACAT GGCCTTCTTC GAGTTTAACA GCCGCCTGGA ATCCATCCTC 
AGCAAAGCCT ACGTGTACAG GGTCATCAGG ACCACAGCCT ACCTTCTCTA CAGCCTGCAT 
TTGAATTCCT GTCTTTATTA CTGGGCATCG GCCTATCAGG GCCTCGGCTC CACTCACTGG 
GTTTACGATG GCGTGGGAAA CAGTTATATT CGCTGTTACT ACTTTGCTGT GAAGACCCTC 
ATCACCATCG GGGGGCTGCC TGACCCCAAG ACACTCTTTG AAATTGTCTT CCAGCTGCTG 
AATTATTTCA CGGGCGTCTT TGCTTTCTCT GTGATGATCG GACAGATGAG AGATGTGGTA 
GGGGCCGCCA CCGCCGGACA GACCTACTAC CGCAGCTGCA TGGACAGCAC GGTGAAGTAC 
ATGAATTTCT ACAAGATCCC CAAGTCCGTG CAGAACCGCG TCAAGACCTG GTACGAGTAC 
ACCTGGCACT CGCAAGGCAT GCTGGATGAG TCAGAGCTGA TGGTGCAGCT TCCAGACAAG 
ATGCGGCTGG ACCTCGCCAT CGACGTGAAC TACAACATCG TTAGCAAAGT CGCACTCTTT 
CAGGGCTGTG ACCGGCAGAT GATCTTTGAC ATGCTGAAGA GGCTTCGCTC TGTTGTCTAC 
CTGCCCAACG ACTATGTGTG CAAGAAGGGG GAGATCGGCC GTGAGATGTA CATCATCCAG 
GCAGGGCAAG TGCAGGTCTT GGGCGGCCCT GATGGGAAAT CTGTGCTGGT GACGCTGAAA 
GCTGGATCTG TGTTTGGAGA AATAAGCTTG CTGGCTGTTG GGGGCGGGAA CCGGCGCACG 
GCCAACGTGG TGGCGCACGG GTTTACCAAC CTCTTCATCC TGGATAAGAA GGACCTGAAT 
GAGATTTTGG TGCATTATCC TGAGTCTCAG AAGTTACTCC GGAAGAAAGC CAGGCGCATG 
CTGAGAAGCA ACAATAAGCC CAAGGAGGAG AAGAGCGTGC TGATCCTTCC ACCCCGGGCG 
GGCACCCCAA AGCTCTTCAA CGCTGCCCTC GCTATGACAG GAAAGATGGG TGGCAAGGGG 
GCAAAAGGCG GCAAACTTGC TCACCTCCGG GCCCGGCTCA AAGAACTGGC CGCGCTGGAG 
GCGGCTGCAA AGCAGCAAGA GTTGGTGGAA CAGGCCAAGA GCTCGCAAGA CGTCAAGGGA 
GAGGAAGGCT CCGCCGCCCC AGACCAGCAC ACGCACCCAA AGGAGGCCGC CACCGACCCA 
CCCGCGCCCC GGACGCCCCC CGAGCCCCCG GGGTCTCCAC CGAGCTCTCC ACCGCCTGCC 
TCCCTTGGGA GGCCGGAGGG AGAGGAGGAG GGGCCGGCCG AGCCCGAAGA GCACTCGGTG 
AGGATCTGCA TGAGCCCGGG CCCGGAGCCG GGAGAGCAGA TCCTGTCGGT GAAGATGCCG 
GAGGAAAGGG AGGAGAAGGC GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Patients and animal models of CNG?1-deficient retinitis pigmentosa support gene augmentation approach.
The Journal of clinical investigation128(1)190-206(2018 Jan)
Petersen-Jones SM,Occelli LM,Winkler PA,Lee W,Sparrow JR,Tsukikawa M,Boye SL,Chiodo V,Capasso JE,Becirovic E,Sch?n C,Seeliger MW,Levin AV,Michalakis S,Hauswirth WW,Tsang SH

Run of homozygosity analysis reveals a novel nonsense variant of the CNGB1 gene involved in retinitis pigmentosa 45.
Ophthalmic genetics37(3)357-9(2016 09)
Fradin M,Colin E,Hannouche-Bared D,Audo I,Sahel JA,Biskup S,Carr? W,Ziegler A,Wilhelm C,Guichet A,Odent S,Bonneau D

Varying the GARP2-to-RDS Ratio Leads to Defects in Rim Formation and Rod and Cone Function.
Investigative ophthalmology & visual science56(13)8187-98(2015 Dec)
Chakraborty D,Conley SM,DeRamus ML,Pittler SJ,Naash MI

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CNGB1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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