Hecw1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_017315649.1	XP_017171138.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_017315651.1	XP_017171140.1	E3 ubiquitin-protein ligase HECW1 isoform X9
XM_006516809.4	XP_006516872.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_006516813.4	XP_006516876.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_006516814.4	XP_006516877.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_006516816.4	XP_006516879.1	E3 ubiquitin-protein ligase HECW1 isoform X7
NM_001081348.3	NP_001074817.3	E3 ubiquitin-protein ligase HECW1
XM_006516810.3	XP_006516873.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_006516811.1	XP_006516874.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_006516812.3	XP_006516875.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_006516816.3	XP_006516879.1	E3 ubiquitin-protein ligase HECW1 isoform X7
XM_006516811.2	XP_006516874.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_006516812.4	XP_006516875.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_006516813.3	XP_006516876.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_006516814.3	XP_006516877.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_017315650.1	XP_017171139.1	E3 ubiquitin-protein ligase HECW1 isoform X8
XM_006516815.4	XP_006516878.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017315649.2	XP_017171138.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_006516815.3	XP_006516878.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017315650.2	XP_017171139.1	E3 ubiquitin-protein ligase HECW1 isoform X8
XM_006516809.3	XP_006516872.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_006516810.4	XP_006516873.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_017315651.2	XP_017171140.1	E3 ubiquitin-protein ligase HECW1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02184	XM_006516812.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu02184	XM_006516811.2 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu02184	XM_006516810.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48477	XM_006516809.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48478	XM_006516813.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48479	XM_006516814.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48480	XM_006516815.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48481	XM_006516816.4 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78556	XM_017315649.2 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78557	XM_017315650.2 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu78558	XM_017315651.2 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu02184	NM_001081348.3 Latest version!	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu48481	XM_006516816.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu02184	XM_006516811.1	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu02184	XM_006516812.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu48478	XM_006516813.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu48479	XM_006516814.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu78556	XM_017315649.1	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48480	XM_006516815.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu78557	XM_017315650.1	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu48477	XM_006516809.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu02184	XM_006516810.3	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu78558	XM_017315651.1	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

Hecw1 ( XM_006516811.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516874.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	XM_006516811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516874.1
CDS	195..5009
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516811.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516811.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516812.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516875.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

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CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	XM_006516812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516812.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516875.1
CDS	9417..14231
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516812.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516812.4

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516810.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516873.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	XM_006516810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516810.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516873.1
CDS	997..5811
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516810.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516810.4

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516809.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516872.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

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CloneID	OMu48477
Clone ID Related Accession (Same CDS sequence)	XM_006516809.4 , XM_006516809.3
Accession Version	XM_006516809.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516809.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGGTGAATGT 
CCTATACTCC ATAATTCCCA GCCAATAAGC CAACTTCCTT CCTTGAGGCC TGAACATCAT 
CACTACCCAA CAATCGATGA GCCTCTTCCA CCAAACTGGG AGGCTCGAAT TGACAGCCAT 
GGACGGGTCT TTTATGTGGA CCATATAAAT CGCACAACCA CCTGGCAGCG ACCATCGATG 
GCCCCTACCC CCGATGGAAT GATCAGATCC GGGTCTGTCC ACCAGATGGA GCAGCTTAAC 
AGAAGGTATC AAAACATCCA GAGGACCATG GCAACAGAGA GAGCTGAAGA AGATTCTGGC 
AATCAAAACT CTGAGCAAAT ACCAGACGGA GGAGGAGGAG GTGGTGGTGG GAGTGACTCG 
GAAGCAGAAT CATCCCAATC AAGCTTAGAT CTGAGGAGAG AAGGGTCACT TTCTCCTGTC 
AACTCACAGA AGGTCACCTT GCTGCTGCAG TCTCCAGCGG TCAAGTTCAT CACCAATCCT 
GAGTTCTTCA CTGTGTTGCA TGCCAATTAT AGTGCCTACA GGGTTTTCAC AAGCAGCACT 
TGCCTGAAGC ATATGATTTT AAAAGTCCGA AGAGATGCTC GAAATTTTGA GCGCTATCAG 
CACAACCGGG ACCTGGTGAA TTTCATCAAC ATGTTTGCAG ATACTCGGCT GGAACTGCCC 
CGGGGTTGGG AGATCAAAAC TGATCATCAG GGAAAGTCTT TCTTCGTGGA CCACAACAGT 
CGAGCTACTA CTTTCATAGA TCCCCGAATC CCTCTTCAGA ATGGCCGTCT TCCCAATCAT 
CTAACCCACC GACAGCACCT CCAGAGGCTC CGGAGTTACA GTGCTGGGGA GGCCTCAGAA 
GTCTCTAGAA ACAGAGGAGC ATCTTTGCTG GCCAGACCAG GACACAGCCT AATAGCTGCT 
ATTCGAAGCC AACATCAACA TGAATCCTTG CCACTGGCAT ACAATGACAA GATTGTGGCA 
TTTCTTCGCC AGCCAAACAT TTTTGAAATG CTACAAGAAC GTCAGCCAAG CTTGGCAAGA 
AACCACACAC TCAGGGAGAA AATCCATTAT ATTCGGACAG AGGGCAATCA TGGGCTTGAT 
AAATTGTCCT GTGATGCAGA TCTAGTCATT TTGCTGAGTC TCTTTGAAGA AGAGATCATG 
TCCTATGTTC CCCTGCAATC TGCCTTCCAC CCTGGATATA GCTTCTCTCC CCGCTGTTCA 
CCCTGTTCCT CACCTCAGAA CTCCCCAGGT TTACAGAGAG CCAGCGCAAG AGCCCCTTCA 
CCATACCGAA GGGATTTTGA AGCTAAGCTC CGCAATTTCT ACCGAAAACT GGAAGCCAAA 
GGATTTGGAC AGGGTCCGGG TAAAATTAAG CTCATCATCC GTCGAGACCA CTTGTTGGAG 
GGGACCTTCA ATCAAGTGAT GGCATACTCA AGGAAGGAGC TCCAGAGAAA CAAGCTCTAC 
ATCACCTTTG TTGGAGAGGA GGGGCTGGAC TACAGTGGCC CTTCTCGAGA GTTCTTCTTC 
CTGTTGTCTC AGGAGCTCTT CAACCCTTAC TATGGACTCT TTGAGTATTC TGCAAATGAC 
ACATACACAG TACAGATCAG CCCCATGTCA GCATTTGTAG AAAACTATCT TGAATGGTTT 
AGATTCAGTG GTCGTATCCT GGGCCTAGCT CTGATCCATC AGTACCTCCT GGACGCTTTC 
TTCACAAGAC CCTTCTATAA GGGACTCCTG AAGCTGCCAT GTGATCTGAG TGACCTAGAA 
TATTTGGATG AGGAATTCCA CCAGAGTTTA CAGTGGATGA AGGACAACAA CATCACAGAC 
ATTCTAGATC TCACCTTCAC TGTTAATGAA GAAGTTTTTG GACAGGTAAC AGAAAGAGAG 
TTGAAGTCTG GAGGCGCCAA CACGCAGGTG ACTGAGAAAA ACAAGAAGGA ATACATAGAG 
AGGATGGTGA AGTGGCGTGT GGAGCGTGGT GTGGTACAGC AGACGGAGGC ATTGCTCAGG 
GGTTTCTATG AGGTTGTAGA CTCAAGGCTC GTCTCAGTGT TTGATGCGAG GGAACTGGAG 
CTGGTGATCG CTGGAACTGC TGAAATCGAC CTGAATGACT GGAGGAATAA CACAGAGTAC 
CGGGGAGGTT ATCACGATGG CCACCTTGTG ATCCGCTGGT TCTGGGCTGC AGTGGAACGC 
TTCAATAATG AGCAGAGATT GCGATTACTT CAGTTTGTGA CAGGAACATC AAGTGTGCCC 
TATGAAGGCT TTGCAGCTCT GCGGGGAAGC AATGGGCTTC GACGTTTCTG CATAGAAAAG 
TGGGGGAAGA TTACATCTCT TCCCAGGGCA CATACGTGCT TCAACCGATT GGATCTCCCA 
CCATACCCTT CATATTCCAT GTTGTATGAA AAGCTGTTAA CAGCAGTAGA AGAAACCAGC 
ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516872.1
CDS	437..5254
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516809.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516809.4

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGGTGAATGT 
CCTATACTCC ATAATTCCCA GCCAATAAGC CAACTTCCTT CCTTGAGGCC TGAACATCAT 
CACTACCCAA CAATCGATGA GCCTCTTCCA CCAAACTGGG AGGCTCGAAT TGACAGCCAT 
GGACGGGTCT TTTATGTGGA CCATATAAAT CGCACAACCA CCTGGCAGCG ACCATCGATG 
GCCCCTACCC CCGATGGAAT GATCAGATCC GGGTCTGTCC ACCAGATGGA GCAGCTTAAC 
AGAAGGTATC AAAACATCCA GAGGACCATG GCAACAGAGA GAGCTGAAGA AGATTCTGGC 
AATCAAAACT CTGAGCAAAT ACCAGACGGA GGAGGAGGAG GTGGTGGTGG GAGTGACTCG 
GAAGCAGAAT CATCCCAATC AAGCTTAGAT CTGAGGAGAG AAGGGTCACT TTCTCCTGTC 
AACTCACAGA AGGTCACCTT GCTGCTGCAG TCTCCAGCGG TCAAGTTCAT CACCAATCCT 
GAGTTCTTCA CTGTGTTGCA TGCCAATTAT AGTGCCTACA GGGTTTTCAC AAGCAGCACT 
TGCCTGAAGC ATATGATTTT AAAAGTCCGA AGAGATGCTC GAAATTTTGA GCGCTATCAG 
CACAACCGGG ACCTGGTGAA TTTCATCAAC ATGTTTGCAG ATACTCGGCT GGAACTGCCC 
CGGGGTTGGG AGATCAAAAC TGATCATCAG GGAAAGTCTT TCTTCGTGGA CCACAACAGT 
CGAGCTACTA CTTTCATAGA TCCCCGAATC CCTCTTCAGA ATGGCCGTCT TCCCAATCAT 
CTAACCCACC GACAGCACCT CCAGAGGCTC CGGAGTTACA GTGCTGGGGA GGCCTCAGAA 
GTCTCTAGAA ACAGAGGAGC ATCTTTGCTG GCCAGACCAG GACACAGCCT AATAGCTGCT 
ATTCGAAGCC AACATCAACA TGAATCCTTG CCACTGGCAT ACAATGACAA GATTGTGGCA 
TTTCTTCGCC AGCCAAACAT TTTTGAAATG CTACAAGAAC GTCAGCCAAG CTTGGCAAGA 
AACCACACAC TCAGGGAGAA AATCCATTAT ATTCGGACAG AGGGCAATCA TGGGCTTGAT 
AAATTGTCCT GTGATGCAGA TCTAGTCATT TTGCTGAGTC TCTTTGAAGA AGAGATCATG 
TCCTATGTTC CCCTGCAATC TGCCTTCCAC CCTGGATATA GCTTCTCTCC CCGCTGTTCA 
CCCTGTTCCT CACCTCAGAA CTCCCCAGGT TTACAGAGAG CCAGCGCAAG AGCCCCTTCA 
CCATACCGAA GGGATTTTGA AGCTAAGCTC CGCAATTTCT ACCGAAAACT GGAAGCCAAA 
GGATTTGGAC AGGGTCCGGG TAAAATTAAG CTCATCATCC GTCGAGACCA CTTGTTGGAG 
GGGACCTTCA ATCAAGTGAT GGCATACTCA AGGAAGGAGC TCCAGAGAAA CAAGCTCTAC 
ATCACCTTTG TTGGAGAGGA GGGGCTGGAC TACAGTGGCC CTTCTCGAGA GTTCTTCTTC 
CTGTTGTCTC AGGAGCTCTT CAACCCTTAC TATGGACTCT TTGAGTATTC TGCAAATGAC 
ACATACACAG TACAGATCAG CCCCATGTCA GCATTTGTAG AAAACTATCT TGAATGGTTT 
AGATTCAGTG GTCGTATCCT GGGCCTAGCT CTGATCCATC AGTACCTCCT GGACGCTTTC 
TTCACAAGAC CCTTCTATAA GGGACTCCTG AAGCTGCCAT GTGATCTGAG TGACCTAGAA 
TATTTGGATG AGGAATTCCA CCAGAGTTTA CAGTGGATGA AGGACAACAA CATCACAGAC 
ATTCTAGATC TCACCTTCAC TGTTAATGAA GAAGTTTTTG GACAGGTAAC AGAAAGAGAG 
TTGAAGTCTG GAGGCGCCAA CACGCAGGTG ACTGAGAAAA ACAAGAAGGA ATACATAGAG 
AGGATGGTGA AGTGGCGTGT GGAGCGTGGT GTGGTACAGC AGACGGAGGC ATTGCTCAGG 
GGTTTCTATG AGGTTGTAGA CTCAAGGCTC GTCTCAGTGT TTGATGCGAG GGAACTGGAG 
CTGGTGATCG CTGGAACTGC TGAAATCGAC CTGAATGACT GGAGGAATAA CACAGAGTAC 
CGGGGAGGTT ATCACGATGG CCACCTTGTG ATCCGCTGGT TCTGGGCTGC AGTGGAACGC 
TTCAATAATG AGCAGAGATT GCGATTACTT CAGTTTGTGA CAGGAACATC AAGTGTGCCC 
TATGAAGGCT TTGCAGCTCT GCGGGGAAGC AATGGGCTTC GACGTTTCTG CATAGAAAAG 
TGGGGGAAGA TTACATCTCT TCCCAGGGCA CATACGTGCT TCAACCGATT GGATCTCCCA 
CCATACCCTT CATATTCCAT GTTGTATGAA AAGCTGTTAA CAGCAGTAGA AGAAACCAGC 
ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516813.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516876.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.

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CloneID	OMu48478
Clone ID Related Accession (Same CDS sequence)	XM_006516813.4 , XM_006516813.3
Accession Version	XM_006516813.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516813.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAATAAGCCA ACTTCCTTCC 
TTGAGGCCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516876.1
CDS	437..5212
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516813.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516813.4

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAATAAGCCA ACTTCCTTCC 
TTGAGGCCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516814.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516877.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.

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CloneID	OMu48479
Clone ID Related Accession (Same CDS sequence)	XM_006516814.4 , XM_006516814.3
Accession Version	XM_006516814.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516814.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGGTGA ATGTCCTATA 
CTCCATAATT CCCAGCCAAT AAGCCAACTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GAATTGACAG CCATGGACGG 
GTCTTTTATG TGGACCATAT AAATCGCACA ACCACCTGGC AGCGACCATC GATGGCCCCT 
ACCCCCGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGGAC CATGGCAACA GAGAGAGCTG AAGAAGATTC TGGCAATCAA 
AACTCTGAGC AAATACCAGA CGGAGGAGGA GGAGGTGGTG GTGGGAGTGA CTCGGAAGCA 
GAATCATCCC AATCAAGCTT AGATCTGAGG AGAGAAGGGT CACTTTCTCC TGTCAACTCA 
CAGAAGGTCA CCTTGCTGCT GCAGTCTCCA GCGGTCAAGT TCATCACCAA TCCTGAGTTC 
TTCACTGTGT TGCATGCCAA TTATAGTGCC TACAGGGTTT TCACAAGCAG CACTTGCCTG 
AAGCATATGA TTTTAAAAGT CCGAAGAGAT GCTCGAAATT TTGAGCGCTA TCAGCACAAC 
CGGGACCTGG TGAATTTCAT CAACATGTTT GCAGATACTC GGCTGGAACT GCCCCGGGGT 
TGGGAGATCA AAACTGATCA TCAGGGAAAG TCTTTCTTCG TGGACCACAA CAGTCGAGCT 
ACTACTTTCA TAGATCCCCG AATCCCTCTT CAGAATGGCC GTCTTCCCAA TCATCTAACC 
CACCGACAGC ACCTCCAGAG GCTCCGGAGT TACAGTGCTG GGGAGGCCTC AGAAGTCTCT 
AGAAACAGAG GAGCATCTTT GCTGGCCAGA CCAGGACACA GCCTAATAGC TGCTATTCGA 
AGCCAACATC AACATGAATC CTTGCCACTG GCATACAATG ACAAGATTGT GGCATTTCTT 
CGCCAGCCAA ACATTTTTGA AATGCTACAA GAACGTCAGC CAAGCTTGGC AAGAAACCAC 
ACACTCAGGG AGAAAATCCA TTATATTCGG ACAGAGGGCA ATCATGGGCT TGATAAATTG 
TCCTGTGATG CAGATCTAGT CATTTTGCTG AGTCTCTTTG AAGAAGAGAT CATGTCCTAT 
GTTCCCCTGC AATCTGCCTT CCACCCTGGA TATAGCTTCT CTCCCCGCTG TTCACCCTGT 
TCCTCACCTC AGAACTCCCC AGGTTTACAG AGAGCCAGCG CAAGAGCCCC TTCACCATAC 
CGAAGGGATT TTGAAGCTAA GCTCCGCAAT TTCTACCGAA AACTGGAAGC CAAAGGATTT 
GGACAGGGTC CGGGTAAAAT TAAGCTCATC ATCCGTCGAG ACCACTTGTT GGAGGGGACC 
TTCAATCAAG TGATGGCATA CTCAAGGAAG GAGCTCCAGA GAAACAAGCT CTACATCACC 
TTTGTTGGAG AGGAGGGGCT GGACTACAGT GGCCCTTCTC GAGAGTTCTT CTTCCTGTTG 
TCTCAGGAGC TCTTCAACCC TTACTATGGA CTCTTTGAGT ATTCTGCAAA TGACACATAC 
ACAGTACAGA TCAGCCCCAT GTCAGCATTT GTAGAAAACT ATCTTGAATG GTTTAGATTC 
AGTGGTCGTA TCCTGGGCCT AGCTCTGATC CATCAGTACC TCCTGGACGC TTTCTTCACA 
AGACCCTTCT ATAAGGGACT CCTGAAGCTG CCATGTGATC TGAGTGACCT AGAATATTTG 
GATGAGGAAT TCCACCAGAG TTTACAGTGG ATGAAGGACA ACAACATCAC AGACATTCTA 
GATCTCACCT TCACTGTTAA TGAAGAAGTT TTTGGACAGG TAACAGAAAG AGAGTTGAAG 
TCTGGAGGCG CCAACACGCA GGTGACTGAG AAAAACAAGA AGGAATACAT AGAGAGGATG 
GTGAAGTGGC GTGTGGAGCG TGGTGTGGTA CAGCAGACGG AGGCATTGCT CAGGGGTTTC 
TATGAGGTTG TAGACTCAAG GCTCGTCTCA GTGTTTGATG CGAGGGAACT GGAGCTGGTG 
ATCGCTGGAA CTGCTGAAAT CGACCTGAAT GACTGGAGGA ATAACACAGA GTACCGGGGA 
GGTTATCACG ATGGCCACCT TGTGATCCGC TGGTTCTGGG CTGCAGTGGA ACGCTTCAAT 
AATGAGCAGA GATTGCGATT ACTTCAGTTT GTGACAGGAA CATCAAGTGT GCCCTATGAA 
GGCTTTGCAG CTCTGCGGGG AAGCAATGGG CTTCGACGTT TCTGCATAGA AAAGTGGGGG 
AAGATTACAT CTCTTCCCAG GGCACATACG TGCTTCAACC GATTGGATCT CCCACCATAC 
CCTTCATATT CCATGTTGTA TGAAAAGCTG TTAACAGCAG TAGAAGAAAC CAGCACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516877.1
CDS	419..5170
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516814.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516814.4

ORF Insert Sequence:

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGGTGA ATGTCCTATA 
CTCCATAATT CCCAGCCAAT AAGCCAACTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GAATTGACAG CCATGGACGG 
GTCTTTTATG TGGACCATAT AAATCGCACA ACCACCTGGC AGCGACCATC GATGGCCCCT 
ACCCCCGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGGAC CATGGCAACA GAGAGAGCTG AAGAAGATTC TGGCAATCAA 
AACTCTGAGC AAATACCAGA CGGAGGAGGA GGAGGTGGTG GTGGGAGTGA CTCGGAAGCA 
GAATCATCCC AATCAAGCTT AGATCTGAGG AGAGAAGGGT CACTTTCTCC TGTCAACTCA 
CAGAAGGTCA CCTTGCTGCT GCAGTCTCCA GCGGTCAAGT TCATCACCAA TCCTGAGTTC 
TTCACTGTGT TGCATGCCAA TTATAGTGCC TACAGGGTTT TCACAAGCAG CACTTGCCTG 
AAGCATATGA TTTTAAAAGT CCGAAGAGAT GCTCGAAATT TTGAGCGCTA TCAGCACAAC 
CGGGACCTGG TGAATTTCAT CAACATGTTT GCAGATACTC GGCTGGAACT GCCCCGGGGT 
TGGGAGATCA AAACTGATCA TCAGGGAAAG TCTTTCTTCG TGGACCACAA CAGTCGAGCT 
ACTACTTTCA TAGATCCCCG AATCCCTCTT CAGAATGGCC GTCTTCCCAA TCATCTAACC 
CACCGACAGC ACCTCCAGAG GCTCCGGAGT TACAGTGCTG GGGAGGCCTC AGAAGTCTCT 
AGAAACAGAG GAGCATCTTT GCTGGCCAGA CCAGGACACA GCCTAATAGC TGCTATTCGA 
AGCCAACATC AACATGAATC CTTGCCACTG GCATACAATG ACAAGATTGT GGCATTTCTT 
CGCCAGCCAA ACATTTTTGA AATGCTACAA GAACGTCAGC CAAGCTTGGC AAGAAACCAC 
ACACTCAGGG AGAAAATCCA TTATATTCGG ACAGAGGGCA ATCATGGGCT TGATAAATTG 
TCCTGTGATG CAGATCTAGT CATTTTGCTG AGTCTCTTTG AAGAAGAGAT CATGTCCTAT 
GTTCCCCTGC AATCTGCCTT CCACCCTGGA TATAGCTTCT CTCCCCGCTG TTCACCCTGT 
TCCTCACCTC AGAACTCCCC AGGTTTACAG AGAGCCAGCG CAAGAGCCCC TTCACCATAC 
CGAAGGGATT TTGAAGCTAA GCTCCGCAAT TTCTACCGAA AACTGGAAGC CAAAGGATTT 
GGACAGGGTC CGGGTAAAAT TAAGCTCATC ATCCGTCGAG ACCACTTGTT GGAGGGGACC 
TTCAATCAAG TGATGGCATA CTCAAGGAAG GAGCTCCAGA GAAACAAGCT CTACATCACC 
TTTGTTGGAG AGGAGGGGCT GGACTACAGT GGCCCTTCTC GAGAGTTCTT CTTCCTGTTG 
TCTCAGGAGC TCTTCAACCC TTACTATGGA CTCTTTGAGT ATTCTGCAAA TGACACATAC 
ACAGTACAGA TCAGCCCCAT GTCAGCATTT GTAGAAAACT ATCTTGAATG GTTTAGATTC 
AGTGGTCGTA TCCTGGGCCT AGCTCTGATC CATCAGTACC TCCTGGACGC TTTCTTCACA 
AGACCCTTCT ATAAGGGACT CCTGAAGCTG CCATGTGATC TGAGTGACCT AGAATATTTG 
GATGAGGAAT TCCACCAGAG TTTACAGTGG ATGAAGGACA ACAACATCAC AGACATTCTA 
GATCTCACCT TCACTGTTAA TGAAGAAGTT TTTGGACAGG TAACAGAAAG AGAGTTGAAG 
TCTGGAGGCG CCAACACGCA GGTGACTGAG AAAAACAAGA AGGAATACAT AGAGAGGATG 
GTGAAGTGGC GTGTGGAGCG TGGTGTGGTA CAGCAGACGG AGGCATTGCT CAGGGGTTTC 
TATGAGGTTG TAGACTCAAG GCTCGTCTCA GTGTTTGATG CGAGGGAACT GGAGCTGGTG 
ATCGCTGGAA CTGCTGAAAT CGACCTGAAT GACTGGAGGA ATAACACAGA GTACCGGGGA 
GGTTATCACG ATGGCCACCT TGTGATCCGC TGGTTCTGGG CTGCAGTGGA ACGCTTCAAT 
AATGAGCAGA GATTGCGATT ACTTCAGTTT GTGACAGGAA CATCAAGTGT GCCCTATGAA 
GGCTTTGCAG CTCTGCGGGG AAGCAATGGG CTTCGACGTT TCTGCATAGA AAAGTGGGGG 
AAGATTACAT CTCTTCCCAG GGCACATACG TGCTTCAACC GATTGGATCT CCCACCATAC 
CCTTCATATT CCATGTTGTA TGAAAAGCTG TTAACAGCAG TAGAAGAAAC CAGCACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516815.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516878.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.

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CloneID	OMu48480
Clone ID Related Accession (Same CDS sequence)	XM_006516815.4 , XM_006516815.3
Accession Version	XM_006516815.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516815.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516878.1
CDS	437..5152
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516815.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516815.4

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516816.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516879.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X9, mRNA.

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CloneID	OMu48481
Clone ID Related Accession (Same CDS sequence)	XM_006516816.4 , XM_006516816.3
Accession Version	XM_006516816.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006516816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG ACTGGGAGGC TCGAATTGAC AGCCATGGAC GGGTCTTTTA TGTGGACCAT 
ATAAATCGCA CAACCACCTG GCAGCGACCA TCGATGGCCC CTACCCCCGA TGGAATGATC 
AGATCCGGGT CTGTCCACCA GATGGAGCAG CTTAACAGAA GGTATCAAAA CATCCAGAGG 
ACCATGGCAA CAGAGAGAGC TGAAGAAGAT TCTGGCAATC AAAACTCTGA GCAAATACCA 
GACGGAGGAG GAGGAGGTGG TGGTGGGAGT GACTCGGAAG CAGAATCATC CCAATCAAGC 
TTAGATCTGA GGAGAGAAGG GTCACTTTCT CCTGTCAACT CACAGAAGGT CACCTTGCTG 
CTGCAGTCTC CAGCGGTCAA GTTCATCACC AATCCTGAGT TCTTCACTGT GTTGCATGCC 
AATTATAGTG CCTACAGGGT TTTCACAAGC AGCACTTGCC TGAAGCATAT GATTTTAAAA 
GTCCGAAGAG ATGCTCGAAA TTTTGAGCGC TATCAGCACA ACCGGGACCT GGTGAATTTC 
ATCAACATGT TTGCAGATAC TCGGCTGGAA CTGCCCCGGG GTTGGGAGAT CAAAACTGAT 
CATCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACTACTTT CATAGATCCC 
CGAATCCCTC TTCAGAATGG CCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGGCTCCGGA GTTACAGTGC TGGGGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCATCT 
TTGCTGGCCA GACCAGGACA CAGCCTAATA GCTGCTATTC GAAGCCAACA TCAACATGAA 
TCCTTGCCAC TGGCATACAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTAC AAGAACGTCA GCCAAGCTTG GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTATATTC GGACAGAGGG CAATCATGGG CTTGATAAAT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATCATGTCCT ATGTTCCCCT GCAATCTGCC 
TTCCACCCTG GATATAGCTT CTCTCCCCGC TGTTCACCCT GTTCCTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCACCAT ACCGAAGGGA TTTTGAAGCT 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGACAGGG TCCGGGTAAA 
ATTAAGCTCA TCATCCGTCG AGACCACTTG TTGGAGGGGA CCTTCAATCA AGTGATGGCA 
TACTCAAGGA AGGAGCTCCA GAGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGG 
CTGGACTACA GTGGCCCTTC TCGAGAGTTC TTCTTCCTGT TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTATTCTGCA AATGACACAT ACACAGTACA GATCAGCCCC 
ATGTCAGCAT TTGTAGAAAA CTATCTTGAA TGGTTTAGAT TCAGTGGTCG TATCCTGGGC 
CTAGCTCTGA TCCATCAGTA CCTCCTGGAC GCTTTCTTCA CAAGACCCTT CTATAAGGGA 
CTCCTGAAGC TGCCATGTGA TCTGAGTGAC CTAGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTACAGT GGATGAAGGA CAACAACATC ACAGACATTC TAGATCTCAC CTTCACTGTT 
AATGAAGAAG TTTTTGGACA GGTAACAGAA AGAGAGTTGA AGTCTGGAGG CGCCAACACG 
CAGGTGACTG AGAAAAACAA GAAGGAATAC ATAGAGAGGA TGGTGAAGTG GCGTGTGGAG 
CGTGGTGTGG TACAGCAGAC GGAGGCATTG CTCAGGGGTT TCTATGAGGT TGTAGACTCA 
AGGCTCGTCT CAGTGTTTGA TGCGAGGGAA CTGGAGCTGG TGATCGCTGG AACTGCTGAA 
ATCGACCTGA ATGACTGGAG GAATAACACA GAGTACCGGG GAGGTTATCA CGATGGCCAC 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAACGCTTCA ATAATGAGCA GAGATTGCGA 
TTACTTCAGT TTGTGACAGG AACATCAAGT GTGCCCTATG AAGGCTTTGC AGCTCTGCGG 
GGAAGCAATG GGCTTCGACG TTTCTGCATA GAAAAGTGGG GGAAGATTAC ATCTCTTCCC 
AGGGCACATA CGTGCTTCAA CCGATTGGAT CTCCCACCAT ACCCTTCATA TTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516879.1
CDS	437..5110
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516816.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516816.4

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG ACTGGGAGGC TCGAATTGAC AGCCATGGAC GGGTCTTTTA TGTGGACCAT 
ATAAATCGCA CAACCACCTG GCAGCGACCA TCGATGGCCC CTACCCCCGA TGGAATGATC 
AGATCCGGGT CTGTCCACCA GATGGAGCAG CTTAACAGAA GGTATCAAAA CATCCAGAGG 
ACCATGGCAA CAGAGAGAGC TGAAGAAGAT TCTGGCAATC AAAACTCTGA GCAAATACCA 
GACGGAGGAG GAGGAGGTGG TGGTGGGAGT GACTCGGAAG CAGAATCATC CCAATCAAGC 
TTAGATCTGA GGAGAGAAGG GTCACTTTCT CCTGTCAACT CACAGAAGGT CACCTTGCTG 
CTGCAGTCTC CAGCGGTCAA GTTCATCACC AATCCTGAGT TCTTCACTGT GTTGCATGCC 
AATTATAGTG CCTACAGGGT TTTCACAAGC AGCACTTGCC TGAAGCATAT GATTTTAAAA 
GTCCGAAGAG ATGCTCGAAA TTTTGAGCGC TATCAGCACA ACCGGGACCT GGTGAATTTC 
ATCAACATGT TTGCAGATAC TCGGCTGGAA CTGCCCCGGG GTTGGGAGAT CAAAACTGAT 
CATCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACTACTTT CATAGATCCC 
CGAATCCCTC TTCAGAATGG CCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGGCTCCGGA GTTACAGTGC TGGGGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCATCT 
TTGCTGGCCA GACCAGGACA CAGCCTAATA GCTGCTATTC GAAGCCAACA TCAACATGAA 
TCCTTGCCAC TGGCATACAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTAC AAGAACGTCA GCCAAGCTTG GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTATATTC GGACAGAGGG CAATCATGGG CTTGATAAAT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATCATGTCCT ATGTTCCCCT GCAATCTGCC 
TTCCACCCTG GATATAGCTT CTCTCCCCGC TGTTCACCCT GTTCCTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCACCAT ACCGAAGGGA TTTTGAAGCT 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGACAGGG TCCGGGTAAA 
ATTAAGCTCA TCATCCGTCG AGACCACTTG TTGGAGGGGA CCTTCAATCA AGTGATGGCA 
TACTCAAGGA AGGAGCTCCA GAGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGG 
CTGGACTACA GTGGCCCTTC TCGAGAGTTC TTCTTCCTGT TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTATTCTGCA AATGACACAT ACACAGTACA GATCAGCCCC 
ATGTCAGCAT TTGTAGAAAA CTATCTTGAA TGGTTTAGAT TCAGTGGTCG TATCCTGGGC 
CTAGCTCTGA TCCATCAGTA CCTCCTGGAC GCTTTCTTCA CAAGACCCTT CTATAAGGGA 
CTCCTGAAGC TGCCATGTGA TCTGAGTGAC CTAGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTACAGT GGATGAAGGA CAACAACATC ACAGACATTC TAGATCTCAC CTTCACTGTT 
AATGAAGAAG TTTTTGGACA GGTAACAGAA AGAGAGTTGA AGTCTGGAGG CGCCAACACG 
CAGGTGACTG AGAAAAACAA GAAGGAATAC ATAGAGAGGA TGGTGAAGTG GCGTGTGGAG 
CGTGGTGTGG TACAGCAGAC GGAGGCATTG CTCAGGGGTT TCTATGAGGT TGTAGACTCA 
AGGCTCGTCT CAGTGTTTGA TGCGAGGGAA CTGGAGCTGG TGATCGCTGG AACTGCTGAA 
ATCGACCTGA ATGACTGGAG GAATAACACA GAGTACCGGG GAGGTTATCA CGATGGCCAC 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAACGCTTCA ATAATGAGCA GAGATTGCGA 
TTACTTCAGT TTGTGACAGG AACATCAAGT GTGCCCTATG AAGGCTTTGC AGCTCTGCGG 
GGAAGCAATG GGCTTCGACG TTTCTGCATA GAAAAGTGGG GGAAGATTAC ATCTCTTCCC 
AGGGCACATA CGTGCTTCAA CCGATTGGAT CTCCCACCAT ACCCTTCATA TTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017315649.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171138.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu78556
Clone ID Related Accession (Same CDS sequence)	XM_017315649.1 , XM_017315649.2
Accession Version	XM_017315649.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGA CTGGGAGGCT 
CGAATTGACA GCCATGGACG GGTCTTTTAT GTGGACCATA TAAATCGCAC AACCACCTGG 
CAGCGACCAT CGATGGCCCC TACCCCCGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGGA CCATGGCAAC AGAGAGAGCT 
GAAGAAGATT CTGGCAATCA AAACTCTGAG CAAATACCAG ACGGAGGAGG AGGAGGTGGT 
GGTGGGAGTG ACTCGGAAGC AGAATCATCC CAATCAAGCT TAGATCTGAG GAGAGAAGGG 
TCACTTTCTC CTGTCAACTC ACAGAAGGTC ACCTTGCTGC TGCAGTCTCC AGCGGTCAAG 
TTCATCACCA ATCCTGAGTT CTTCACTGTG TTGCATGCCA ATTATAGTGC CTACAGGGTT 
TTCACAAGCA GCACTTGCCT GAAGCATATG ATTTTAAAAG TCCGAAGAGA TGCTCGAAAT 
TTTGAGCGCT ATCAGCACAA CCGGGACCTG GTGAATTTCA TCAACATGTT TGCAGATACT 
CGGCTGGAAC TGCCCCGGGG TTGGGAGATC AAAACTGATC ATCAGGGAAA GTCTTTCTTC 
GTGGACCACA ACAGTCGAGC TACTACTTTC ATAGATCCCC GAATCCCTCT TCAGAATGGC 
CGTCTTCCCA ATCATCTAAC CCACCGACAG CACCTCCAGA GGCTCCGGAG TTACAGTGCT 
GGGGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCATCTT TGCTGGCCAG ACCAGGACAC 
AGCCTAATAG CTGCTATTCG AAGCCAACAT CAACATGAAT CCTTGCCACT GGCATACAAT 
GACAAGATTG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAACGTCAG 
CCAAGCTTGG CAAGAAACCA CACACTCAGG GAGAAAATCC ATTATATTCG GACAGAGGGC 
AATCATGGGC TTGATAAATT GTCCTGTGAT GCAGATCTAG TCATTTTGCT GAGTCTCTTT 
GAAGAAGAGA TCATGTCCTA TGTTCCCCTG CAATCTGCCT TCCACCCTGG ATATAGCTTC 
TCTCCCCGCT GTTCACCCTG TTCCTCACCT CAGAACTCCC CAGGTTTACA GAGAGCCAGC 
GCAAGAGCCC CTTCACCATA CCGAAGGGAT TTTGAAGCTA AGCTCCGCAA TTTCTACCGA 
AAACTGGAAG CCAAAGGATT TGGACAGGGT CCGGGTAAAA TTAAGCTCAT CATCCGTCGA 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCAT ACTCAAGGAA GGAGCTCCAG 
AGAAACAAGC TCTACATCAC CTTTGTTGGA GAGGAGGGGC TGGACTACAG TGGCCCTTCT 
CGAGAGTTCT TCTTCCTGTT GTCTCAGGAG CTCTTCAACC CTTACTATGG ACTCTTTGAG 
TATTCTGCAA ATGACACATA CACAGTACAG ATCAGCCCCA TGTCAGCATT TGTAGAAAAC 
TATCTTGAAT GGTTTAGATT CAGTGGTCGT ATCCTGGGCC TAGCTCTGAT CCATCAGTAC 
CTCCTGGACG CTTTCTTCAC AAGACCCTTC TATAAGGGAC TCCTGAAGCT GCCATGTGAT 
CTGAGTGACC TAGAATATTT GGATGAGGAA TTCCACCAGA GTTTACAGTG GATGAAGGAC 
AACAACATCA CAGACATTCT AGATCTCACC TTCACTGTTA ATGAAGAAGT TTTTGGACAG 
GTAACAGAAA GAGAGTTGAA GTCTGGAGGC GCCAACACGC AGGTGACTGA GAAAAACAAG 
AAGGAATACA TAGAGAGGAT GGTGAAGTGG CGTGTGGAGC GTGGTGTGGT ACAGCAGACG 
GAGGCATTGC TCAGGGGTTT CTATGAGGTT GTAGACTCAA GGCTCGTCTC AGTGTTTGAT 
GCGAGGGAAC TGGAGCTGGT GATCGCTGGA ACTGCTGAAA TCGACCTGAA TGACTGGAGG 
AATAACACAG AGTACCGGGG AGGTTATCAC GATGGCCACC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AACGCTTCAA TAATGAGCAG AGATTGCGAT TACTTCAGTT TGTGACAGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCA GCTCTGCGGG GAAGCAATGG GCTTCGACGT 
TTCTGCATAG AAAAGTGGGG GAAGATTACA TCTCTTCCCA GGGCACATAC GTGCTTCAAC 
CGATTGGATC TCCCACCATA CCCTTCATAT TCCATGTTGT ATGAAAAGCT GTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171138.1
CDS	471..5183
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017315649.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315649.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGA CTGGGAGGCT 
CGAATTGACA GCCATGGACG GGTCTTTTAT GTGGACCATA TAAATCGCAC AACCACCTGG 
CAGCGACCAT CGATGGCCCC TACCCCCGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGGA CCATGGCAAC AGAGAGAGCT 
GAAGAAGATT CTGGCAATCA AAACTCTGAG CAAATACCAG ACGGAGGAGG AGGAGGTGGT 
GGTGGGAGTG ACTCGGAAGC AGAATCATCC CAATCAAGCT TAGATCTGAG GAGAGAAGGG 
TCACTTTCTC CTGTCAACTC ACAGAAGGTC ACCTTGCTGC TGCAGTCTCC AGCGGTCAAG 
TTCATCACCA ATCCTGAGTT CTTCACTGTG TTGCATGCCA ATTATAGTGC CTACAGGGTT 
TTCACAAGCA GCACTTGCCT GAAGCATATG ATTTTAAAAG TCCGAAGAGA TGCTCGAAAT 
TTTGAGCGCT ATCAGCACAA CCGGGACCTG GTGAATTTCA TCAACATGTT TGCAGATACT 
CGGCTGGAAC TGCCCCGGGG TTGGGAGATC AAAACTGATC ATCAGGGAAA GTCTTTCTTC 
GTGGACCACA ACAGTCGAGC TACTACTTTC ATAGATCCCC GAATCCCTCT TCAGAATGGC 
CGTCTTCCCA ATCATCTAAC CCACCGACAG CACCTCCAGA GGCTCCGGAG TTACAGTGCT 
GGGGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCATCTT TGCTGGCCAG ACCAGGACAC 
AGCCTAATAG CTGCTATTCG AAGCCAACAT CAACATGAAT CCTTGCCACT GGCATACAAT 
GACAAGATTG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAACGTCAG 
CCAAGCTTGG CAAGAAACCA CACACTCAGG GAGAAAATCC ATTATATTCG GACAGAGGGC 
AATCATGGGC TTGATAAATT GTCCTGTGAT GCAGATCTAG TCATTTTGCT GAGTCTCTTT 
GAAGAAGAGA TCATGTCCTA TGTTCCCCTG CAATCTGCCT TCCACCCTGG ATATAGCTTC 
TCTCCCCGCT GTTCACCCTG TTCCTCACCT CAGAACTCCC CAGGTTTACA GAGAGCCAGC 
GCAAGAGCCC CTTCACCATA CCGAAGGGAT TTTGAAGCTA AGCTCCGCAA TTTCTACCGA 
AAACTGGAAG CCAAAGGATT TGGACAGGGT CCGGGTAAAA TTAAGCTCAT CATCCGTCGA 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCAT ACTCAAGGAA GGAGCTCCAG 
AGAAACAAGC TCTACATCAC CTTTGTTGGA GAGGAGGGGC TGGACTACAG TGGCCCTTCT 
CGAGAGTTCT TCTTCCTGTT GTCTCAGGAG CTCTTCAACC CTTACTATGG ACTCTTTGAG 
TATTCTGCAA ATGACACATA CACAGTACAG ATCAGCCCCA TGTCAGCATT TGTAGAAAAC 
TATCTTGAAT GGTTTAGATT CAGTGGTCGT ATCCTGGGCC TAGCTCTGAT CCATCAGTAC 
CTCCTGGACG CTTTCTTCAC AAGACCCTTC TATAAGGGAC TCCTGAAGCT GCCATGTGAT 
CTGAGTGACC TAGAATATTT GGATGAGGAA TTCCACCAGA GTTTACAGTG GATGAAGGAC 
AACAACATCA CAGACATTCT AGATCTCACC TTCACTGTTA ATGAAGAAGT TTTTGGACAG 
GTAACAGAAA GAGAGTTGAA GTCTGGAGGC GCCAACACGC AGGTGACTGA GAAAAACAAG 
AAGGAATACA TAGAGAGGAT GGTGAAGTGG CGTGTGGAGC GTGGTGTGGT ACAGCAGACG 
GAGGCATTGC TCAGGGGTTT CTATGAGGTT GTAGACTCAA GGCTCGTCTC AGTGTTTGAT 
GCGAGGGAAC TGGAGCTGGT GATCGCTGGA ACTGCTGAAA TCGACCTGAA TGACTGGAGG 
AATAACACAG AGTACCGGGG AGGTTATCAC GATGGCCACC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AACGCTTCAA TAATGAGCAG AGATTGCGAT TACTTCAGTT TGTGACAGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCA GCTCTGCGGG GAAGCAATGG GCTTCGACGT 
TTCTGCATAG AAAAGTGGGG GAAGATTACA TCTCTTCCCA GGGCACATAC GTGCTTCAAC 
CGATTGGATC TCCCACCATA CCCTTCATAT TCCATGTTGT ATGAAAAGCT GTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017315649.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171138.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X8, mRNA.

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CloneID	OMu78556
Clone ID Related Accession (Same CDS sequence)	XM_017315649.1 , XM_017315649.2
Accession Version	XM_017315649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGA CTGGGAGGCT 
CGAATTGACA GCCATGGACG GGTCTTTTAT GTGGACCATA TAAATCGCAC AACCACCTGG 
CAGCGACCAT CGATGGCCCC TACCCCCGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGGA CCATGGCAAC AGAGAGAGCT 
GAAGAAGATT CTGGCAATCA AAACTCTGAG CAAATACCAG ACGGAGGAGG AGGAGGTGGT 
GGTGGGAGTG ACTCGGAAGC AGAATCATCC CAATCAAGCT TAGATCTGAG GAGAGAAGGG 
TCACTTTCTC CTGTCAACTC ACAGAAGGTC ACCTTGCTGC TGCAGTCTCC AGCGGTCAAG 
TTCATCACCA ATCCTGAGTT CTTCACTGTG TTGCATGCCA ATTATAGTGC CTACAGGGTT 
TTCACAAGCA GCACTTGCCT GAAGCATATG ATTTTAAAAG TCCGAAGAGA TGCTCGAAAT 
TTTGAGCGCT ATCAGCACAA CCGGGACCTG GTGAATTTCA TCAACATGTT TGCAGATACT 
CGGCTGGAAC TGCCCCGGGG TTGGGAGATC AAAACTGATC ATCAGGGAAA GTCTTTCTTC 
GTGGACCACA ACAGTCGAGC TACTACTTTC ATAGATCCCC GAATCCCTCT TCAGAATGGC 
CGTCTTCCCA ATCATCTAAC CCACCGACAG CACCTCCAGA GGCTCCGGAG TTACAGTGCT 
GGGGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCATCTT TGCTGGCCAG ACCAGGACAC 
AGCCTAATAG CTGCTATTCG AAGCCAACAT CAACATGAAT CCTTGCCACT GGCATACAAT 
GACAAGATTG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAACGTCAG 
CCAAGCTTGG CAAGAAACCA CACACTCAGG GAGAAAATCC ATTATATTCG GACAGAGGGC 
AATCATGGGC TTGATAAATT GTCCTGTGAT GCAGATCTAG TCATTTTGCT GAGTCTCTTT 
GAAGAAGAGA TCATGTCCTA TGTTCCCCTG CAATCTGCCT TCCACCCTGG ATATAGCTTC 
TCTCCCCGCT GTTCACCCTG TTCCTCACCT CAGAACTCCC CAGGTTTACA GAGAGCCAGC 
GCAAGAGCCC CTTCACCATA CCGAAGGGAT TTTGAAGCTA AGCTCCGCAA TTTCTACCGA 
AAACTGGAAG CCAAAGGATT TGGACAGGGT CCGGGTAAAA TTAAGCTCAT CATCCGTCGA 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCAT ACTCAAGGAA GGAGCTCCAG 
AGAAACAAGC TCTACATCAC CTTTGTTGGA GAGGAGGGGC TGGACTACAG TGGCCCTTCT 
CGAGAGTTCT TCTTCCTGTT GTCTCAGGAG CTCTTCAACC CTTACTATGG ACTCTTTGAG 
TATTCTGCAA ATGACACATA CACAGTACAG ATCAGCCCCA TGTCAGCATT TGTAGAAAAC 
TATCTTGAAT GGTTTAGATT CAGTGGTCGT ATCCTGGGCC TAGCTCTGAT CCATCAGTAC 
CTCCTGGACG CTTTCTTCAC AAGACCCTTC TATAAGGGAC TCCTGAAGCT GCCATGTGAT 
CTGAGTGACC TAGAATATTT GGATGAGGAA TTCCACCAGA GTTTACAGTG GATGAAGGAC 
AACAACATCA CAGACATTCT AGATCTCACC TTCACTGTTA ATGAAGAAGT TTTTGGACAG 
GTAACAGAAA GAGAGTTGAA GTCTGGAGGC GCCAACACGC AGGTGACTGA GAAAAACAAG 
AAGGAATACA TAGAGAGGAT GGTGAAGTGG CGTGTGGAGC GTGGTGTGGT ACAGCAGACG 
GAGGCATTGC TCAGGGGTTT CTATGAGGTT GTAGACTCAA GGCTCGTCTC AGTGTTTGAT 
GCGAGGGAAC TGGAGCTGGT GATCGCTGGA ACTGCTGAAA TCGACCTGAA TGACTGGAGG 
AATAACACAG AGTACCGGGG AGGTTATCAC GATGGCCACC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AACGCTTCAA TAATGAGCAG AGATTGCGAT TACTTCAGTT TGTGACAGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCA GCTCTGCGGG GAAGCAATGG GCTTCGACGT 
TTCTGCATAG AAAAGTGGGG GAAGATTACA TCTCTTCCCA GGGCACATAC GTGCTTCAAC 
CGATTGGATC TCCCACCATA CCCTTCATAT TCCATGTTGT ATGAAAAGCT GTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171138.1
CDS	997..5709
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017315649.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315649.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGA CTGGGAGGCT 
CGAATTGACA GCCATGGACG GGTCTTTTAT GTGGACCATA TAAATCGCAC AACCACCTGG 
CAGCGACCAT CGATGGCCCC TACCCCCGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGGA CCATGGCAAC AGAGAGAGCT 
GAAGAAGATT CTGGCAATCA AAACTCTGAG CAAATACCAG ACGGAGGAGG AGGAGGTGGT 
GGTGGGAGTG ACTCGGAAGC AGAATCATCC CAATCAAGCT TAGATCTGAG GAGAGAAGGG 
TCACTTTCTC CTGTCAACTC ACAGAAGGTC ACCTTGCTGC TGCAGTCTCC AGCGGTCAAG 
TTCATCACCA ATCCTGAGTT CTTCACTGTG TTGCATGCCA ATTATAGTGC CTACAGGGTT 
TTCACAAGCA GCACTTGCCT GAAGCATATG ATTTTAAAAG TCCGAAGAGA TGCTCGAAAT 
TTTGAGCGCT ATCAGCACAA CCGGGACCTG GTGAATTTCA TCAACATGTT TGCAGATACT 
CGGCTGGAAC TGCCCCGGGG TTGGGAGATC AAAACTGATC ATCAGGGAAA GTCTTTCTTC 
GTGGACCACA ACAGTCGAGC TACTACTTTC ATAGATCCCC GAATCCCTCT TCAGAATGGC 
CGTCTTCCCA ATCATCTAAC CCACCGACAG CACCTCCAGA GGCTCCGGAG TTACAGTGCT 
GGGGAGGCCT CAGAAGTCTC TAGAAACAGA GGAGCATCTT TGCTGGCCAG ACCAGGACAC 
AGCCTAATAG CTGCTATTCG AAGCCAACAT CAACATGAAT CCTTGCCACT GGCATACAAT 
GACAAGATTG TGGCATTTCT TCGCCAGCCA AACATTTTTG AAATGCTACA AGAACGTCAG 
CCAAGCTTGG CAAGAAACCA CACACTCAGG GAGAAAATCC ATTATATTCG GACAGAGGGC 
AATCATGGGC TTGATAAATT GTCCTGTGAT GCAGATCTAG TCATTTTGCT GAGTCTCTTT 
GAAGAAGAGA TCATGTCCTA TGTTCCCCTG CAATCTGCCT TCCACCCTGG ATATAGCTTC 
TCTCCCCGCT GTTCACCCTG TTCCTCACCT CAGAACTCCC CAGGTTTACA GAGAGCCAGC 
GCAAGAGCCC CTTCACCATA CCGAAGGGAT TTTGAAGCTA AGCTCCGCAA TTTCTACCGA 
AAACTGGAAG CCAAAGGATT TGGACAGGGT CCGGGTAAAA TTAAGCTCAT CATCCGTCGA 
GACCACTTGT TGGAGGGGAC CTTCAATCAA GTGATGGCAT ACTCAAGGAA GGAGCTCCAG 
AGAAACAAGC TCTACATCAC CTTTGTTGGA GAGGAGGGGC TGGACTACAG TGGCCCTTCT 
CGAGAGTTCT TCTTCCTGTT GTCTCAGGAG CTCTTCAACC CTTACTATGG ACTCTTTGAG 
TATTCTGCAA ATGACACATA CACAGTACAG ATCAGCCCCA TGTCAGCATT TGTAGAAAAC 
TATCTTGAAT GGTTTAGATT CAGTGGTCGT ATCCTGGGCC TAGCTCTGAT CCATCAGTAC 
CTCCTGGACG CTTTCTTCAC AAGACCCTTC TATAAGGGAC TCCTGAAGCT GCCATGTGAT 
CTGAGTGACC TAGAATATTT GGATGAGGAA TTCCACCAGA GTTTACAGTG GATGAAGGAC 
AACAACATCA CAGACATTCT AGATCTCACC TTCACTGTTA ATGAAGAAGT TTTTGGACAG 
GTAACAGAAA GAGAGTTGAA GTCTGGAGGC GCCAACACGC AGGTGACTGA GAAAAACAAG 
AAGGAATACA TAGAGAGGAT GGTGAAGTGG CGTGTGGAGC GTGGTGTGGT ACAGCAGACG 
GAGGCATTGC TCAGGGGTTT CTATGAGGTT GTAGACTCAA GGCTCGTCTC AGTGTTTGAT 
GCGAGGGAAC TGGAGCTGGT GATCGCTGGA ACTGCTGAAA TCGACCTGAA TGACTGGAGG 
AATAACACAG AGTACCGGGG AGGTTATCAC GATGGCCACC TTGTGATCCG CTGGTTCTGG 
GCTGCAGTGG AACGCTTCAA TAATGAGCAG AGATTGCGAT TACTTCAGTT TGTGACAGGA 
ACATCAAGTG TGCCCTATGA AGGCTTTGCA GCTCTGCGGG GAAGCAATGG GCTTCGACGT 
TTCTGCATAG AAAAGTGGGG GAAGATTACA TCTCTTCCCA GGGCACATAC GTGCTTCAAC 
CGATTGGATC TCCCACCATA CCCTTCATAT TCCATGTTGT ATGAAAAGCT GTTAACAGCA 
GTAGAAGAAA CCAGCACCTT TGGACTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017315650.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171139.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X10, mRNA.

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CloneID	OMu78557
Clone ID Related Accession (Same CDS sequence)	XM_017315650.1 , XM_017315650.2
Accession Version	XM_017315650.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGACT GGGAGGCTCG AATTGACAGC CATGGACGGG TCTTTTATGT GGACCATATA 
AATCGCACAA CCACCTGGCA GCGACCATCG ATGGCCCCTA CCCCCGATGG AATGATCAGA 
TCCGGGTCTG TCCACCAGAT GGAGCAGCTT AACAGAAGGT ATCAAAACAT CCAGAGGACC 
ATGGCAACAG AGAGAGCTGA AGAAGATTCT GGCAATCAAA ACTCTGAGCA AATACCAGAC 
GGAGGAGGAG GAGGTGGTGG TGGGAGTGAC TCGGAAGCAG AATCATCCCA ATCAAGCTTA 
GATCTGAGGA GAGAAGGGTC ACTTTCTCCT GTCAACTCAC AGAAGGTCAC CTTGCTGCTG 
CAGTCTCCAG CGGTCAAGTT CATCACCAAT CCTGAGTTCT TCACTGTGTT GCATGCCAAT 
TATAGTGCCT ACAGGGTTTT CACAAGCAGC ACTTGCCTGA AGCATATGAT TTTAAAAGTC 
CGAAGAGATG CTCGAAATTT TGAGCGCTAT CAGCACAACC GGGACCTGGT GAATTTCATC 
AACATGTTTG CAGATACTCG GCTGGAACTG CCCCGGGGTT GGGAGATCAA AACTGATCAT 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CTACTTTCAT AGATCCCCGA 
ATCCCTCTTC AGAATGGCCG TCTTCCCAAT CATCTAACCC ACCGACAGCA CCTCCAGAGG 
CTCCGGAGTT ACAGTGCTGG GGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCATCTTTG 
CTGGCCAGAC CAGGACACAG CCTAATAGCT GCTATTCGAA GCCAACATCA ACATGAATCC 
TTGCCACTGG CATACAATGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AACGTCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CAGAGGGCAA TCATGGGCTT GATAAATTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA AGAAGAGATC ATGTCCTATG TTCCCCTGCA ATCTGCCTTC 
CACCCTGGAT ATAGCTTCTC TCCCCGCTGT TCACCCTGTT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGCGC AAGAGCCCCT TCACCATACC GAAGGGATTT TGAAGCTAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GACAGGGTCC GGGTAAAATT 
AAGCTCATCA TCCGTCGAGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCATAC 
TCAAGGAAGG AGCTCCAGAG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGGCTG 
GACTACAGTG GCCCTTCTCG AGAGTTCTTC TTCCTGTTGT CTCAGGAGCT CTTCAACCCT 
TACTATGGAC TCTTTGAGTA TTCTGCAAAT GACACATACA CAGTACAGAT CAGCCCCATG 
TCAGCATTTG TAGAAAACTA TCTTGAATGG TTTAGATTCA GTGGTCGTAT CCTGGGCCTA 
GCTCTGATCC ATCAGTACCT CCTGGACGCT TTCTTCACAA GACCCTTCTA TAAGGGACTC 
CTGAAGCTGC CATGTGATCT GAGTGACCTA GAATATTTGG ATGAGGAATT CCACCAGAGT 
TTACAGTGGA TGAAGGACAA CAACATCACA GACATTCTAG ATCTCACCTT CACTGTTAAT 
GAAGAAGTTT TTGGACAGGT AACAGAAAGA GAGTTGAAGT CTGGAGGCGC CAACACGCAG 
GTGACTGAGA AAAACAAGAA GGAATACATA GAGAGGATGG TGAAGTGGCG TGTGGAGCGT 
GGTGTGGTAC AGCAGACGGA GGCATTGCTC AGGGGTTTCT ATGAGGTTGT AGACTCAAGG 
CTCGTCTCAG TGTTTGATGC GAGGGAACTG GAGCTGGTGA TCGCTGGAAC TGCTGAAATC 
GACCTGAATG ACTGGAGGAA TAACACAGAG TACCGGGGAG GTTATCACGA TGGCCACCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAA CGCTTCAATA ATGAGCAGAG ATTGCGATTA 
CTTCAGTTTG TGACAGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCAGC TCTGCGGGGA 
AGCAATGGGC TTCGACGTTT CTGCATAGAA AAGTGGGGGA AGATTACATC TCTTCCCAGG 
GCACATACGT GCTTCAACCG ATTGGATCTC CCACCATACC CTTCATATTC CATGTTGTAT 
GAAAAGCTGT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171139.1
CDS	471..5141
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017315650.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315650.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGACT GGGAGGCTCG AATTGACAGC CATGGACGGG TCTTTTATGT GGACCATATA 
AATCGCACAA CCACCTGGCA GCGACCATCG ATGGCCCCTA CCCCCGATGG AATGATCAGA 
TCCGGGTCTG TCCACCAGAT GGAGCAGCTT AACAGAAGGT ATCAAAACAT CCAGAGGACC 
ATGGCAACAG AGAGAGCTGA AGAAGATTCT GGCAATCAAA ACTCTGAGCA AATACCAGAC 
GGAGGAGGAG GAGGTGGTGG TGGGAGTGAC TCGGAAGCAG AATCATCCCA ATCAAGCTTA 
GATCTGAGGA GAGAAGGGTC ACTTTCTCCT GTCAACTCAC AGAAGGTCAC CTTGCTGCTG 
CAGTCTCCAG CGGTCAAGTT CATCACCAAT CCTGAGTTCT TCACTGTGTT GCATGCCAAT 
TATAGTGCCT ACAGGGTTTT CACAAGCAGC ACTTGCCTGA AGCATATGAT TTTAAAAGTC 
CGAAGAGATG CTCGAAATTT TGAGCGCTAT CAGCACAACC GGGACCTGGT GAATTTCATC 
AACATGTTTG CAGATACTCG GCTGGAACTG CCCCGGGGTT GGGAGATCAA AACTGATCAT 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CTACTTTCAT AGATCCCCGA 
ATCCCTCTTC AGAATGGCCG TCTTCCCAAT CATCTAACCC ACCGACAGCA CCTCCAGAGG 
CTCCGGAGTT ACAGTGCTGG GGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCATCTTTG 
CTGGCCAGAC CAGGACACAG CCTAATAGCT GCTATTCGAA GCCAACATCA ACATGAATCC 
TTGCCACTGG CATACAATGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AACGTCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CAGAGGGCAA TCATGGGCTT GATAAATTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA AGAAGAGATC ATGTCCTATG TTCCCCTGCA ATCTGCCTTC 
CACCCTGGAT ATAGCTTCTC TCCCCGCTGT TCACCCTGTT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGCGC AAGAGCCCCT TCACCATACC GAAGGGATTT TGAAGCTAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GACAGGGTCC GGGTAAAATT 
AAGCTCATCA TCCGTCGAGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCATAC 
TCAAGGAAGG AGCTCCAGAG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGGCTG 
GACTACAGTG GCCCTTCTCG AGAGTTCTTC TTCCTGTTGT CTCAGGAGCT CTTCAACCCT 
TACTATGGAC TCTTTGAGTA TTCTGCAAAT GACACATACA CAGTACAGAT CAGCCCCATG 
TCAGCATTTG TAGAAAACTA TCTTGAATGG TTTAGATTCA GTGGTCGTAT CCTGGGCCTA 
GCTCTGATCC ATCAGTACCT CCTGGACGCT TTCTTCACAA GACCCTTCTA TAAGGGACTC 
CTGAAGCTGC CATGTGATCT GAGTGACCTA GAATATTTGG ATGAGGAATT CCACCAGAGT 
TTACAGTGGA TGAAGGACAA CAACATCACA GACATTCTAG ATCTCACCTT CACTGTTAAT 
GAAGAAGTTT TTGGACAGGT AACAGAAAGA GAGTTGAAGT CTGGAGGCGC CAACACGCAG 
GTGACTGAGA AAAACAAGAA GGAATACATA GAGAGGATGG TGAAGTGGCG TGTGGAGCGT 
GGTGTGGTAC AGCAGACGGA GGCATTGCTC AGGGGTTTCT ATGAGGTTGT AGACTCAAGG 
CTCGTCTCAG TGTTTGATGC GAGGGAACTG GAGCTGGTGA TCGCTGGAAC TGCTGAAATC 
GACCTGAATG ACTGGAGGAA TAACACAGAG TACCGGGGAG GTTATCACGA TGGCCACCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAA CGCTTCAATA ATGAGCAGAG ATTGCGATTA 
CTTCAGTTTG TGACAGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCAGC TCTGCGGGGA 
AGCAATGGGC TTCGACGTTT CTGCATAGAA AAGTGGGGGA AGATTACATC TCTTCCCAGG 
GCACATACGT GCTTCAACCG ATTGGATCTC CCACCATACC CTTCATATTC CATGTTGTAT 
GAAAAGCTGT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017315650.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171139.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X10, mRNA.

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CloneID	OMu78557
Clone ID Related Accession (Same CDS sequence)	XM_017315650.1 , XM_017315650.2
Accession Version	XM_017315650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGACT GGGAGGCTCG AATTGACAGC CATGGACGGG TCTTTTATGT GGACCATATA 
AATCGCACAA CCACCTGGCA GCGACCATCG ATGGCCCCTA CCCCCGATGG AATGATCAGA 
TCCGGGTCTG TCCACCAGAT GGAGCAGCTT AACAGAAGGT ATCAAAACAT CCAGAGGACC 
ATGGCAACAG AGAGAGCTGA AGAAGATTCT GGCAATCAAA ACTCTGAGCA AATACCAGAC 
GGAGGAGGAG GAGGTGGTGG TGGGAGTGAC TCGGAAGCAG AATCATCCCA ATCAAGCTTA 
GATCTGAGGA GAGAAGGGTC ACTTTCTCCT GTCAACTCAC AGAAGGTCAC CTTGCTGCTG 
CAGTCTCCAG CGGTCAAGTT CATCACCAAT CCTGAGTTCT TCACTGTGTT GCATGCCAAT 
TATAGTGCCT ACAGGGTTTT CACAAGCAGC ACTTGCCTGA AGCATATGAT TTTAAAAGTC 
CGAAGAGATG CTCGAAATTT TGAGCGCTAT CAGCACAACC GGGACCTGGT GAATTTCATC 
AACATGTTTG CAGATACTCG GCTGGAACTG CCCCGGGGTT GGGAGATCAA AACTGATCAT 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CTACTTTCAT AGATCCCCGA 
ATCCCTCTTC AGAATGGCCG TCTTCCCAAT CATCTAACCC ACCGACAGCA CCTCCAGAGG 
CTCCGGAGTT ACAGTGCTGG GGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCATCTTTG 
CTGGCCAGAC CAGGACACAG CCTAATAGCT GCTATTCGAA GCCAACATCA ACATGAATCC 
TTGCCACTGG CATACAATGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AACGTCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CAGAGGGCAA TCATGGGCTT GATAAATTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA AGAAGAGATC ATGTCCTATG TTCCCCTGCA ATCTGCCTTC 
CACCCTGGAT ATAGCTTCTC TCCCCGCTGT TCACCCTGTT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGCGC AAGAGCCCCT TCACCATACC GAAGGGATTT TGAAGCTAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GACAGGGTCC GGGTAAAATT 
AAGCTCATCA TCCGTCGAGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCATAC 
TCAAGGAAGG AGCTCCAGAG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGGCTG 
GACTACAGTG GCCCTTCTCG AGAGTTCTTC TTCCTGTTGT CTCAGGAGCT CTTCAACCCT 
TACTATGGAC TCTTTGAGTA TTCTGCAAAT GACACATACA CAGTACAGAT CAGCCCCATG 
TCAGCATTTG TAGAAAACTA TCTTGAATGG TTTAGATTCA GTGGTCGTAT CCTGGGCCTA 
GCTCTGATCC ATCAGTACCT CCTGGACGCT TTCTTCACAA GACCCTTCTA TAAGGGACTC 
CTGAAGCTGC CATGTGATCT GAGTGACCTA GAATATTTGG ATGAGGAATT CCACCAGAGT 
TTACAGTGGA TGAAGGACAA CAACATCACA GACATTCTAG ATCTCACCTT CACTGTTAAT 
GAAGAAGTTT TTGGACAGGT AACAGAAAGA GAGTTGAAGT CTGGAGGCGC CAACACGCAG 
GTGACTGAGA AAAACAAGAA GGAATACATA GAGAGGATGG TGAAGTGGCG TGTGGAGCGT 
GGTGTGGTAC AGCAGACGGA GGCATTGCTC AGGGGTTTCT ATGAGGTTGT AGACTCAAGG 
CTCGTCTCAG TGTTTGATGC GAGGGAACTG GAGCTGGTGA TCGCTGGAAC TGCTGAAATC 
GACCTGAATG ACTGGAGGAA TAACACAGAG TACCGGGGAG GTTATCACGA TGGCCACCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAA CGCTTCAATA ATGAGCAGAG ATTGCGATTA 
CTTCAGTTTG TGACAGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCAGC TCTGCGGGGA 
AGCAATGGGC TTCGACGTTT CTGCATAGAA AAGTGGGGGA AGATTACATC TCTTCCCAGG 
GCACATACGT GCTTCAACCG ATTGGATCTC CCACCATACC CTTCATATTC CATGTTGTAT 
GAAAAGCTGT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171139.1
CDS	997..5667
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017315650.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315650.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGACT GGGAGGCTCG AATTGACAGC CATGGACGGG TCTTTTATGT GGACCATATA 
AATCGCACAA CCACCTGGCA GCGACCATCG ATGGCCCCTA CCCCCGATGG AATGATCAGA 
TCCGGGTCTG TCCACCAGAT GGAGCAGCTT AACAGAAGGT ATCAAAACAT CCAGAGGACC 
ATGGCAACAG AGAGAGCTGA AGAAGATTCT GGCAATCAAA ACTCTGAGCA AATACCAGAC 
GGAGGAGGAG GAGGTGGTGG TGGGAGTGAC TCGGAAGCAG AATCATCCCA ATCAAGCTTA 
GATCTGAGGA GAGAAGGGTC ACTTTCTCCT GTCAACTCAC AGAAGGTCAC CTTGCTGCTG 
CAGTCTCCAG CGGTCAAGTT CATCACCAAT CCTGAGTTCT TCACTGTGTT GCATGCCAAT 
TATAGTGCCT ACAGGGTTTT CACAAGCAGC ACTTGCCTGA AGCATATGAT TTTAAAAGTC 
CGAAGAGATG CTCGAAATTT TGAGCGCTAT CAGCACAACC GGGACCTGGT GAATTTCATC 
AACATGTTTG CAGATACTCG GCTGGAACTG CCCCGGGGTT GGGAGATCAA AACTGATCAT 
CAGGGAAAGT CTTTCTTCGT GGACCACAAC AGTCGAGCTA CTACTTTCAT AGATCCCCGA 
ATCCCTCTTC AGAATGGCCG TCTTCCCAAT CATCTAACCC ACCGACAGCA CCTCCAGAGG 
CTCCGGAGTT ACAGTGCTGG GGAGGCCTCA GAAGTCTCTA GAAACAGAGG AGCATCTTTG 
CTGGCCAGAC CAGGACACAG CCTAATAGCT GCTATTCGAA GCCAACATCA ACATGAATCC 
TTGCCACTGG CATACAATGA CAAGATTGTG GCATTTCTTC GCCAGCCAAA CATTTTTGAA 
ATGCTACAAG AACGTCAGCC AAGCTTGGCA AGAAACCACA CACTCAGGGA GAAAATCCAT 
TATATTCGGA CAGAGGGCAA TCATGGGCTT GATAAATTGT CCTGTGATGC AGATCTAGTC 
ATTTTGCTGA GTCTCTTTGA AGAAGAGATC ATGTCCTATG TTCCCCTGCA ATCTGCCTTC 
CACCCTGGAT ATAGCTTCTC TCCCCGCTGT TCACCCTGTT CCTCACCTCA GAACTCCCCA 
GGTTTACAGA GAGCCAGCGC AAGAGCCCCT TCACCATACC GAAGGGATTT TGAAGCTAAG 
CTCCGCAATT TCTACCGAAA ACTGGAAGCC AAAGGATTTG GACAGGGTCC GGGTAAAATT 
AAGCTCATCA TCCGTCGAGA CCACTTGTTG GAGGGGACCT TCAATCAAGT GATGGCATAC 
TCAAGGAAGG AGCTCCAGAG AAACAAGCTC TACATCACCT TTGTTGGAGA GGAGGGGCTG 
GACTACAGTG GCCCTTCTCG AGAGTTCTTC TTCCTGTTGT CTCAGGAGCT CTTCAACCCT 
TACTATGGAC TCTTTGAGTA TTCTGCAAAT GACACATACA CAGTACAGAT CAGCCCCATG 
TCAGCATTTG TAGAAAACTA TCTTGAATGG TTTAGATTCA GTGGTCGTAT CCTGGGCCTA 
GCTCTGATCC ATCAGTACCT CCTGGACGCT TTCTTCACAA GACCCTTCTA TAAGGGACTC 
CTGAAGCTGC CATGTGATCT GAGTGACCTA GAATATTTGG ATGAGGAATT CCACCAGAGT 
TTACAGTGGA TGAAGGACAA CAACATCACA GACATTCTAG ATCTCACCTT CACTGTTAAT 
GAAGAAGTTT TTGGACAGGT AACAGAAAGA GAGTTGAAGT CTGGAGGCGC CAACACGCAG 
GTGACTGAGA AAAACAAGAA GGAATACATA GAGAGGATGG TGAAGTGGCG TGTGGAGCGT 
GGTGTGGTAC AGCAGACGGA GGCATTGCTC AGGGGTTTCT ATGAGGTTGT AGACTCAAGG 
CTCGTCTCAG TGTTTGATGC GAGGGAACTG GAGCTGGTGA TCGCTGGAAC TGCTGAAATC 
GACCTGAATG ACTGGAGGAA TAACACAGAG TACCGGGGAG GTTATCACGA TGGCCACCTT 
GTGATCCGCT GGTTCTGGGC TGCAGTGGAA CGCTTCAATA ATGAGCAGAG ATTGCGATTA 
CTTCAGTTTG TGACAGGAAC ATCAAGTGTG CCCTATGAAG GCTTTGCAGC TCTGCGGGGA 
AGCAATGGGC TTCGACGTTT CTGCATAGAA AAGTGGGGGA AGATTACATC TCTTCCCAGG 
GCACATACGT GCTTCAACCG ATTGGATCTC CCACCATACC CTTCATATTC CATGTTGTAT 
GAAAAGCTGT TAACAGCAGT AGAAGAAACC AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017315651.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171140.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu78558
Clone ID Related Accession (Same CDS sequence)	XM_017315651.1 , XM_017315651.2
Accession Version	XM_017315651.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGACTG GGAGGCTCGA 
ATTGACAGCC ATGGACGGGT CTTTTATGTG GACCATATAA ATCGCACAAC CACCTGGCAG 
CGACCATCGA TGGCCCCTAC CCCCGATGGA ATGATCAGAT CCGGGTCTGT CCACCAGATG 
GAGCAGCTTA ACAGAAGGTA TCAAAACATC CAGAGGACCA TGGCAACAGA GAGAGCTGAA 
GAAGATTCTG GCAATCAAAA CTCTGAGCAA ATACCAGACG GAGGAGGAGG AGGTGGTGGT 
GGGAGTGACT CGGAAGCAGA ATCATCCCAA TCAAGCTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC GGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTC 
ACAAGCAGCA CTTGCCTGAA GCATATGATT TTAAAAGTCC GAAGAGATGC TCGAAATTTT 
GAGCGCTATC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTCGTG 
GACCACAACA GTCGAGCTAC TACTTTCATA GATCCCCGAA TCCCTCTTCA GAATGGCCGT 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCCGGAGTTA CAGTGCTGGG 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGAAG CCAACATCAA CATGAATCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG CCAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGGCTTG ATAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTGAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT TCCCCTGCAA TCTGCCTTCC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCTCAG AACTCCCCAG GTTTACAGAG AGCCAGCGCA 
AGAGCCCCTT CACCATACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG ACAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAAGTG ATGGCATACT CAAGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACATACAC AGTACAGATC AGCCCCATGT CAGCATTTGT AGAAAACTAT 
CTTGAATGGT TTAGATTCAG TGGTCGTATC CTGGGCCTAG CTCTGATCCA TCAGTACCTC 
CTGGACGCTT TCTTCACAAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGTGATCTG 
AGTGACCTAG AATATTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ACATTCTAGA TCTCACCTTC ACTGTTAATG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGCGCC AACACGCAGG TGACTGAGAA AAACAAGAAG 
GAATACATAG AGAGGATGGT GAAGTGGCGT GTGGAGCGTG GTGTGGTACA GCAGACGGAG 
GCATTGCTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TCGTCTCAGT GTTTGATGCG 
AGGGAACTGG AGCTGGTGAT CGCTGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACAGAGT ACCGGGGAGG TTATCACGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAAC GCTTCAATAA TGAGCAGAGA TTGCGATTAC TTCAGTTTGT GACAGGAACA 
TCAAGTGTGC CCTATGAAGG CTTTGCAGCT CTGCGGGGAA GCAATGGGCT TCGACGTTTC 
TGCATAGAAA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACGTG CTTCAACCGA 
TTGGATCTCC CACCATACCC TTCATATTCC ATGTTGTATG AAAAGCTGTT AACAGCAGTA 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171140.1
CDS	476..5125
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017315651.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315651.1

ORF Insert Sequence:

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGACTG GGAGGCTCGA 
ATTGACAGCC ATGGACGGGT CTTTTATGTG GACCATATAA ATCGCACAAC CACCTGGCAG 
CGACCATCGA TGGCCCCTAC CCCCGATGGA ATGATCAGAT CCGGGTCTGT CCACCAGATG 
GAGCAGCTTA ACAGAAGGTA TCAAAACATC CAGAGGACCA TGGCAACAGA GAGAGCTGAA 
GAAGATTCTG GCAATCAAAA CTCTGAGCAA ATACCAGACG GAGGAGGAGG AGGTGGTGGT 
GGGAGTGACT CGGAAGCAGA ATCATCCCAA TCAAGCTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC GGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTC 
ACAAGCAGCA CTTGCCTGAA GCATATGATT TTAAAAGTCC GAAGAGATGC TCGAAATTTT 
GAGCGCTATC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTCGTG 
GACCACAACA GTCGAGCTAC TACTTTCATA GATCCCCGAA TCCCTCTTCA GAATGGCCGT 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCCGGAGTTA CAGTGCTGGG 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGAAG CCAACATCAA CATGAATCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG CCAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGGCTTG ATAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTGAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT TCCCCTGCAA TCTGCCTTCC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCTCAG AACTCCCCAG GTTTACAGAG AGCCAGCGCA 
AGAGCCCCTT CACCATACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG ACAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAAGTG ATGGCATACT CAAGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACATACAC AGTACAGATC AGCCCCATGT CAGCATTTGT AGAAAACTAT 
CTTGAATGGT TTAGATTCAG TGGTCGTATC CTGGGCCTAG CTCTGATCCA TCAGTACCTC 
CTGGACGCTT TCTTCACAAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGTGATCTG 
AGTGACCTAG AATATTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ACATTCTAGA TCTCACCTTC ACTGTTAATG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGCGCC AACACGCAGG TGACTGAGAA AAACAAGAAG 
GAATACATAG AGAGGATGGT GAAGTGGCGT GTGGAGCGTG GTGTGGTACA GCAGACGGAG 
GCATTGCTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TCGTCTCAGT GTTTGATGCG 
AGGGAACTGG AGCTGGTGAT CGCTGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACAGAGT ACCGGGGAGG TTATCACGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAAC GCTTCAATAA TGAGCAGAGA TTGCGATTAC TTCAGTTTGT GACAGGAACA 
TCAAGTGTGC CCTATGAAGG CTTTGCAGCT CTGCGGGGAA GCAATGGGCT TCGACGTTTC 
TGCATAGAAA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACGTG CTTCAACCGA 
TTGGATCTCC CACCATACCC TTCATATTCC ATGTTGTATG AAAAGCTGTT AACAGCAGTA 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017315651.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171140.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu78558
Clone ID Related Accession (Same CDS sequence)	XM_017315651.1 , XM_017315651.2
Accession Version	XM_017315651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017315651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGACTG GGAGGCTCGA 
ATTGACAGCC ATGGACGGGT CTTTTATGTG GACCATATAA ATCGCACAAC CACCTGGCAG 
CGACCATCGA TGGCCCCTAC CCCCGATGGA ATGATCAGAT CCGGGTCTGT CCACCAGATG 
GAGCAGCTTA ACAGAAGGTA TCAAAACATC CAGAGGACCA TGGCAACAGA GAGAGCTGAA 
GAAGATTCTG GCAATCAAAA CTCTGAGCAA ATACCAGACG GAGGAGGAGG AGGTGGTGGT 
GGGAGTGACT CGGAAGCAGA ATCATCCCAA TCAAGCTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC GGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTC 
ACAAGCAGCA CTTGCCTGAA GCATATGATT TTAAAAGTCC GAAGAGATGC TCGAAATTTT 
GAGCGCTATC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTCGTG 
GACCACAACA GTCGAGCTAC TACTTTCATA GATCCCCGAA TCCCTCTTCA GAATGGCCGT 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCCGGAGTTA CAGTGCTGGG 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGAAG CCAACATCAA CATGAATCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG CCAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGGCTTG ATAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTGAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT TCCCCTGCAA TCTGCCTTCC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCTCAG AACTCCCCAG GTTTACAGAG AGCCAGCGCA 
AGAGCCCCTT CACCATACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG ACAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAAGTG ATGGCATACT CAAGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACATACAC AGTACAGATC AGCCCCATGT CAGCATTTGT AGAAAACTAT 
CTTGAATGGT TTAGATTCAG TGGTCGTATC CTGGGCCTAG CTCTGATCCA TCAGTACCTC 
CTGGACGCTT TCTTCACAAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGTGATCTG 
AGTGACCTAG AATATTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ACATTCTAGA TCTCACCTTC ACTGTTAATG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGCGCC AACACGCAGG TGACTGAGAA AAACAAGAAG 
GAATACATAG AGAGGATGGT GAAGTGGCGT GTGGAGCGTG GTGTGGTACA GCAGACGGAG 
GCATTGCTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TCGTCTCAGT GTTTGATGCG 
AGGGAACTGG AGCTGGTGAT CGCTGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACAGAGT ACCGGGGAGG TTATCACGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAAC GCTTCAATAA TGAGCAGAGA TTGCGATTAC TTCAGTTTGT GACAGGAACA 
TCAAGTGTGC CCTATGAAGG CTTTGCAGCT CTGCGGGGAA GCAATGGGCT TCGACGTTTC 
TGCATAGAAA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACGTG CTTCAACCGA 
TTGGATCTCC CACCATACCC TTCATATTCC ATGTTGTATG AAAAGCTGTT AACAGCAGTA 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171140.1
CDS	419..5068
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017315651.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315651.2

ORF Insert Sequence:

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGACTG GGAGGCTCGA 
ATTGACAGCC ATGGACGGGT CTTTTATGTG GACCATATAA ATCGCACAAC CACCTGGCAG 
CGACCATCGA TGGCCCCTAC CCCCGATGGA ATGATCAGAT CCGGGTCTGT CCACCAGATG 
GAGCAGCTTA ACAGAAGGTA TCAAAACATC CAGAGGACCA TGGCAACAGA GAGAGCTGAA 
GAAGATTCTG GCAATCAAAA CTCTGAGCAA ATACCAGACG GAGGAGGAGG AGGTGGTGGT 
GGGAGTGACT CGGAAGCAGA ATCATCCCAA TCAAGCTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC GGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTC 
ACAAGCAGCA CTTGCCTGAA GCATATGATT TTAAAAGTCC GAAGAGATGC TCGAAATTTT 
GAGCGCTATC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTCGTG 
GACCACAACA GTCGAGCTAC TACTTTCATA GATCCCCGAA TCCCTCTTCA GAATGGCCGT 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCCGGAGTTA CAGTGCTGGG 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGAAG CCAACATCAA CATGAATCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG CCAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGGCTTG ATAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTGAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT TCCCCTGCAA TCTGCCTTCC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCTCAG AACTCCCCAG GTTTACAGAG AGCCAGCGCA 
AGAGCCCCTT CACCATACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG ACAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAAGTG ATGGCATACT CAAGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACATACAC AGTACAGATC AGCCCCATGT CAGCATTTGT AGAAAACTAT 
CTTGAATGGT TTAGATTCAG TGGTCGTATC CTGGGCCTAG CTCTGATCCA TCAGTACCTC 
CTGGACGCTT TCTTCACAAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGTGATCTG 
AGTGACCTAG AATATTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ACATTCTAGA TCTCACCTTC ACTGTTAATG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGCGCC AACACGCAGG TGACTGAGAA AAACAAGAAG 
GAATACATAG AGAGGATGGT GAAGTGGCGT GTGGAGCGTG GTGTGGTACA GCAGACGGAG 
GCATTGCTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TCGTCTCAGT GTTTGATGCG 
AGGGAACTGG AGCTGGTGAT CGCTGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACAGAGT ACCGGGGAGG TTATCACGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAAC GCTTCAATAA TGAGCAGAGA TTGCGATTAC TTCAGTTTGT GACAGGAACA 
TCAAGTGTGC CCTATGAAGG CTTTGCAGCT CTGCGGGGAA GCAATGGGCT TCGACGTTTC 
TGCATAGAAA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACGTG CTTCAACCGA 
TTGGATCTCC CACCATACCC TTCATATTCC ATGTTGTATG AAAAGCTGTT AACAGCAGTA 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( NM_001081348.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074817.3 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), mRNA.

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CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	NM_001081348.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB606770.2, AK047678.1, AB083710.1, AK172932.1 and CT025604.10. On Dec 20, 2007 this sequence version replaced NM_001081348.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849380 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074817.3
CDS	435..5249
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001081348.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081348.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516810.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516873.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

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CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	XM_006516810.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516810.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516873.1
CDS	471..5285
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516810.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516810.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516811.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516874.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

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CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	XM_006516811.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516874.1
CDS	101..4915
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516811.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516811.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516812.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516875.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

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CloneID	OMu02184
Clone ID Related Accession (Same CDS sequence)	NM_001081348.3 , XM_006516810.3 , XM_006516811.1 , XM_006516812.3 , XM_006516811.2 , XM_006516812.4 , XM_006516810.4
Accession Version	XM_006516812.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516875.1
CDS	2817..7631
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516812.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516812.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCGT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA ACCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGCT 
ATCACCATCC CTCGCTCCAC CAGTGACACT GACCTGGTCA CTTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCGATA GGGCACTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA TTATAAGAAC CGTGGAGTCA ATGGTTCTCA CAGAGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG TGGGGCCCTG CGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCAG CCCCTATTTT TAAAGGTATT GGCTCTGAAG AGACTGCACA AAGTCAAGGA 
AGCCGGAGGC TGATCAGCTT CTCCCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCCGACCC TTATCTGAAG ATTTCCATTC AACCTGGGAA ACATAGCATC 
TTCCCAGCCC TCCCTCATCA TGGACAGGAA AGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACCGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CTGTTCAGAG GCTGCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCCGGCT TCCAACTGAC CATGTGAGTG GGCAGCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA TGCAGATGAT GAAGAGATCT CTCTGAGTGC TGAGCCTGAG 
TCGTCAGCAG AAACGCAGGA CAGCATCATG AATAGTATGG TGGGAAACAG CAATGGAGAG 
CCTTCTGGTG ATGCTACAGA ATTCTGCAAG GATGCGAAGC CAGAGTCACC AAGTGAAGGG 
AATGGAGTAA ACAGCTCAGA AAACCAAAAC CAGGAGCATG CCGGGCCAGT TGAGGAAGCG 
GCAGGAGCTA TGGAGGCCAG AGATGGTAGC AATGTCTCTG AAGCACCCGA AGAGCCCGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACCC TGAGTGCAGA AGAAGTGGCC 
GAGGGACTTC CCCTGGATGA GGATTCGCCA TCCTCGCTGT TGCCAGAAGA GAATACAGCT 
CTAGGCAGCA AGGTAGAAGA GGAAACAGTA CCAGAAAATG GAGCCAGAGA GGAAGAAATG 
CAGAAGGGGA AAGATGAAGA AGAGGAAGAA GAGGACGTTT CTACCCTGGA GCAAGGAGAG 
CCTGGACTGG AGCTGAGGGT CTCCGTGAGA AAAAAAAGCA GACCATGTTC CCTACCTGTA 
TCAGAGCTTG AGACAGTGAT TGCATCTGCC TGTGGAGATG CTGAGACCCC GAGAACCCAT 
TATATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGAGAGG AAGCACCACG 
GAGGAGGAAG ATGGCTTGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAGCGAT 
GGGGCTACTC TGTGCATGGC ACCCTCTGAC TGCTCTGGGG GACATTTCTC CAGTTTATCC 
AAAGGCATTG GTGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGCAGC 
CCGTCTTGCT ATAGCACATC TTGCTACAGC AGCTCTTGTT ACAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGTAA CAATCGGTTC GCCAGCCACA CACGCTTCTC CTCTGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA AGAGGAAAAC 
AGTGCATTCG AGTCGGTACC CGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GCCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAAGT 
ACTGAAGGTC TGGAGTCCCC CATGGCAGGT CCTAGCAATA GGAGAGAAGG TGAATGTCCT 
ATACTCCATA ATTCCCAGCC AATAAGCCAA CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAGG CTCGAATTGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TATAAATCGC ACAACCACCT GGCAGCGACC ATCGATGGCC 
CCTACCCCCG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG GACCATGGCA ACAGAGAGAG CTGAAGAAGA TTCTGGCAAT 
CAAAACTCTG AGCAAATACC AGACGGAGGA GGAGGAGGTG GTGGTGGGAG TGACTCGGAA 
GCAGAATCAT CCCAATCAAG CTTAGATCTG AGGAGAGAAG GGTCACTTTC TCCTGTCAAC 
TCACAGAAGG TCACCTTGCT GCTGCAGTCT CCAGCGGTCA AGTTCATCAC CAATCCTGAG 
TTCTTCACTG TGTTGCATGC CAATTATAGT GCCTACAGGG TTTTCACAAG CAGCACTTGC 
CTGAAGCATA TGATTTTAAA AGTCCGAAGA GATGCTCGAA ATTTTGAGCG CTATCAGCAC 
AACCGGGACC TGGTGAATTT CATCAACATG TTTGCAGATA CTCGGCTGGA ACTGCCCCGG 
GGTTGGGAGA TCAAAACTGA TCATCAGGGA AAGTCTTTCT TCGTGGACCA CAACAGTCGA 
GCTACTACTT TCATAGATCC CCGAATCCCT CTTCAGAATG GCCGTCTTCC CAATCATCTA 
ACCCACCGAC AGCACCTCCA GAGGCTCCGG AGTTACAGTG CTGGGGAGGC CTCAGAAGTC 
TCTAGAAACA GAGGAGCATC TTTGCTGGCC AGACCAGGAC ACAGCCTAAT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA ATCCTTGCCA CTGGCATACA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTA CAAGAACGTC AGCCAAGCTT GGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTATATT CGGACAGAGG GCAATCATGG GCTTGATAAA 
TTGTCCTGTG ATGCAGATCT AGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATCATGTCC 
TATGTTCCCC TGCAATCTGC CTTCCACCCT GGATATAGCT TCTCTCCCCG CTGTTCACCC 
TGTTCCTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GCGCAAGAGC CCCTTCACCA 
TACCGAAGGG ATTTTGAAGC TAAGCTCCGC AATTTCTACC GAAAACTGGA AGCCAAAGGA 
TTTGGACAGG GTCCGGGTAA AATTAAGCTC ATCATCCGTC GAGACCACTT GTTGGAGGGG 
ACCTTCAATC AAGTGATGGC ATACTCAAGG AAGGAGCTCC AGAGAAACAA GCTCTACATC 
ACCTTTGTTG GAGAGGAGGG GCTGGACTAC AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG 
TTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTATTCTGC AAATGACACA 
TACACAGTAC AGATCAGCCC CATGTCAGCA TTTGTAGAAA ACTATCTTGA ATGGTTTAGA 
TTCAGTGGTC GTATCCTGGG CCTAGCTCTG ATCCATCAGT ACCTCCTGGA CGCTTTCTTC 
ACAAGACCCT TCTATAAGGG ACTCCTGAAG CTGCCATGTG ATCTGAGTGA CCTAGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTACAG TGGATGAAGG ACAACAACAT CACAGACATT 
CTAGATCTCA CCTTCACTGT TAATGAAGAA GTTTTTGGAC AGGTAACAGA AAGAGAGTTG 
AAGTCTGGAG GCGCCAACAC GCAGGTGACT GAGAAAAACA AGAAGGAATA CATAGAGAGG 
ATGGTGAAGT GGCGTGTGGA GCGTGGTGTG GTACAGCAGA CGGAGGCATT GCTCAGGGGT 
TTCTATGAGG TTGTAGACTC AAGGCTCGTC TCAGTGTTTG ATGCGAGGGA ACTGGAGCTG 
GTGATCGCTG GAACTGCTGA AATCGACCTG AATGACTGGA GGAATAACAC AGAGTACCGG 
GGAGGTTATC ACGATGGCCA CCTTGTGATC CGCTGGTTCT GGGCTGCAGT GGAACGCTTC 
AATAATGAGC AGAGATTGCG ATTACTTCAG TTTGTGACAG GAACATCAAG TGTGCCCTAT 
GAAGGCTTTG CAGCTCTGCG GGGAAGCAAT GGGCTTCGAC GTTTCTGCAT AGAAAAGTGG 
GGGAAGATTA CATCTCTTCC CAGGGCACAT ACGTGCTTCA ACCGATTGGA TCTCCCACCA 
TACCCTTCAT ATTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAAGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516809.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516872.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu48477
Clone ID Related Accession (Same CDS sequence)	XM_006516809.4 , XM_006516809.3
Accession Version	XM_006516809.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGGTGAATGT 
CCTATACTCC ATAATTCCCA GCCAATAAGC CAACTTCCTT CCTTGAGGCC TGAACATCAT 
CACTACCCAA CAATCGATGA GCCTCTTCCA CCAAACTGGG AGGCTCGAAT TGACAGCCAT 
GGACGGGTCT TTTATGTGGA CCATATAAAT CGCACAACCA CCTGGCAGCG ACCATCGATG 
GCCCCTACCC CCGATGGAAT GATCAGATCC GGGTCTGTCC ACCAGATGGA GCAGCTTAAC 
AGAAGGTATC AAAACATCCA GAGGACCATG GCAACAGAGA GAGCTGAAGA AGATTCTGGC 
AATCAAAACT CTGAGCAAAT ACCAGACGGA GGAGGAGGAG GTGGTGGTGG GAGTGACTCG 
GAAGCAGAAT CATCCCAATC AAGCTTAGAT CTGAGGAGAG AAGGGTCACT TTCTCCTGTC 
AACTCACAGA AGGTCACCTT GCTGCTGCAG TCTCCAGCGG TCAAGTTCAT CACCAATCCT 
GAGTTCTTCA CTGTGTTGCA TGCCAATTAT AGTGCCTACA GGGTTTTCAC AAGCAGCACT 
TGCCTGAAGC ATATGATTTT AAAAGTCCGA AGAGATGCTC GAAATTTTGA GCGCTATCAG 
CACAACCGGG ACCTGGTGAA TTTCATCAAC ATGTTTGCAG ATACTCGGCT GGAACTGCCC 
CGGGGTTGGG AGATCAAAAC TGATCATCAG GGAAAGTCTT TCTTCGTGGA CCACAACAGT 
CGAGCTACTA CTTTCATAGA TCCCCGAATC CCTCTTCAGA ATGGCCGTCT TCCCAATCAT 
CTAACCCACC GACAGCACCT CCAGAGGCTC CGGAGTTACA GTGCTGGGGA GGCCTCAGAA 
GTCTCTAGAA ACAGAGGAGC ATCTTTGCTG GCCAGACCAG GACACAGCCT AATAGCTGCT 
ATTCGAAGCC AACATCAACA TGAATCCTTG CCACTGGCAT ACAATGACAA GATTGTGGCA 
TTTCTTCGCC AGCCAAACAT TTTTGAAATG CTACAAGAAC GTCAGCCAAG CTTGGCAAGA 
AACCACACAC TCAGGGAGAA AATCCATTAT ATTCGGACAG AGGGCAATCA TGGGCTTGAT 
AAATTGTCCT GTGATGCAGA TCTAGTCATT TTGCTGAGTC TCTTTGAAGA AGAGATCATG 
TCCTATGTTC CCCTGCAATC TGCCTTCCAC CCTGGATATA GCTTCTCTCC CCGCTGTTCA 
CCCTGTTCCT CACCTCAGAA CTCCCCAGGT TTACAGAGAG CCAGCGCAAG AGCCCCTTCA 
CCATACCGAA GGGATTTTGA AGCTAAGCTC CGCAATTTCT ACCGAAAACT GGAAGCCAAA 
GGATTTGGAC AGGGTCCGGG TAAAATTAAG CTCATCATCC GTCGAGACCA CTTGTTGGAG 
GGGACCTTCA ATCAAGTGAT GGCATACTCA AGGAAGGAGC TCCAGAGAAA CAAGCTCTAC 
ATCACCTTTG TTGGAGAGGA GGGGCTGGAC TACAGTGGCC CTTCTCGAGA GTTCTTCTTC 
CTGTTGTCTC AGGAGCTCTT CAACCCTTAC TATGGACTCT TTGAGTATTC TGCAAATGAC 
ACATACACAG TACAGATCAG CCCCATGTCA GCATTTGTAG AAAACTATCT TGAATGGTTT 
AGATTCAGTG GTCGTATCCT GGGCCTAGCT CTGATCCATC AGTACCTCCT GGACGCTTTC 
TTCACAAGAC CCTTCTATAA GGGACTCCTG AAGCTGCCAT GTGATCTGAG TGACCTAGAA 
TATTTGGATG AGGAATTCCA CCAGAGTTTA CAGTGGATGA AGGACAACAA CATCACAGAC 
ATTCTAGATC TCACCTTCAC TGTTAATGAA GAAGTTTTTG GACAGGTAAC AGAAAGAGAG 
TTGAAGTCTG GAGGCGCCAA CACGCAGGTG ACTGAGAAAA ACAAGAAGGA ATACATAGAG 
AGGATGGTGA AGTGGCGTGT GGAGCGTGGT GTGGTACAGC AGACGGAGGC ATTGCTCAGG 
GGTTTCTATG AGGTTGTAGA CTCAAGGCTC GTCTCAGTGT TTGATGCGAG GGAACTGGAG 
CTGGTGATCG CTGGAACTGC TGAAATCGAC CTGAATGACT GGAGGAATAA CACAGAGTAC 
CGGGGAGGTT ATCACGATGG CCACCTTGTG ATCCGCTGGT TCTGGGCTGC AGTGGAACGC 
TTCAATAATG AGCAGAGATT GCGATTACTT CAGTTTGTGA CAGGAACATC AAGTGTGCCC 
TATGAAGGCT TTGCAGCTCT GCGGGGAAGC AATGGGCTTC GACGTTTCTG CATAGAAAAG 
TGGGGGAAGA TTACATCTCT TCCCAGGGCA CATACGTGCT TCAACCGATT GGATCTCCCA 
CCATACCCTT CATATTCCAT GTTGTATGAA AAGCTGTTAA CAGCAGTAGA AGAAACCAGC 
ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516872.1
CDS	427..5244
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516809.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516809.3

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGGTGAATGT 
CCTATACTCC ATAATTCCCA GCCAATAAGC CAACTTCCTT CCTTGAGGCC TGAACATCAT 
CACTACCCAA CAATCGATGA GCCTCTTCCA CCAAACTGGG AGGCTCGAAT TGACAGCCAT 
GGACGGGTCT TTTATGTGGA CCATATAAAT CGCACAACCA CCTGGCAGCG ACCATCGATG 
GCCCCTACCC CCGATGGAAT GATCAGATCC GGGTCTGTCC ACCAGATGGA GCAGCTTAAC 
AGAAGGTATC AAAACATCCA GAGGACCATG GCAACAGAGA GAGCTGAAGA AGATTCTGGC 
AATCAAAACT CTGAGCAAAT ACCAGACGGA GGAGGAGGAG GTGGTGGTGG GAGTGACTCG 
GAAGCAGAAT CATCCCAATC AAGCTTAGAT CTGAGGAGAG AAGGGTCACT TTCTCCTGTC 
AACTCACAGA AGGTCACCTT GCTGCTGCAG TCTCCAGCGG TCAAGTTCAT CACCAATCCT 
GAGTTCTTCA CTGTGTTGCA TGCCAATTAT AGTGCCTACA GGGTTTTCAC AAGCAGCACT 
TGCCTGAAGC ATATGATTTT AAAAGTCCGA AGAGATGCTC GAAATTTTGA GCGCTATCAG 
CACAACCGGG ACCTGGTGAA TTTCATCAAC ATGTTTGCAG ATACTCGGCT GGAACTGCCC 
CGGGGTTGGG AGATCAAAAC TGATCATCAG GGAAAGTCTT TCTTCGTGGA CCACAACAGT 
CGAGCTACTA CTTTCATAGA TCCCCGAATC CCTCTTCAGA ATGGCCGTCT TCCCAATCAT 
CTAACCCACC GACAGCACCT CCAGAGGCTC CGGAGTTACA GTGCTGGGGA GGCCTCAGAA 
GTCTCTAGAA ACAGAGGAGC ATCTTTGCTG GCCAGACCAG GACACAGCCT AATAGCTGCT 
ATTCGAAGCC AACATCAACA TGAATCCTTG CCACTGGCAT ACAATGACAA GATTGTGGCA 
TTTCTTCGCC AGCCAAACAT TTTTGAAATG CTACAAGAAC GTCAGCCAAG CTTGGCAAGA 
AACCACACAC TCAGGGAGAA AATCCATTAT ATTCGGACAG AGGGCAATCA TGGGCTTGAT 
AAATTGTCCT GTGATGCAGA TCTAGTCATT TTGCTGAGTC TCTTTGAAGA AGAGATCATG 
TCCTATGTTC CCCTGCAATC TGCCTTCCAC CCTGGATATA GCTTCTCTCC CCGCTGTTCA 
CCCTGTTCCT CACCTCAGAA CTCCCCAGGT TTACAGAGAG CCAGCGCAAG AGCCCCTTCA 
CCATACCGAA GGGATTTTGA AGCTAAGCTC CGCAATTTCT ACCGAAAACT GGAAGCCAAA 
GGATTTGGAC AGGGTCCGGG TAAAATTAAG CTCATCATCC GTCGAGACCA CTTGTTGGAG 
GGGACCTTCA ATCAAGTGAT GGCATACTCA AGGAAGGAGC TCCAGAGAAA CAAGCTCTAC 
ATCACCTTTG TTGGAGAGGA GGGGCTGGAC TACAGTGGCC CTTCTCGAGA GTTCTTCTTC 
CTGTTGTCTC AGGAGCTCTT CAACCCTTAC TATGGACTCT TTGAGTATTC TGCAAATGAC 
ACATACACAG TACAGATCAG CCCCATGTCA GCATTTGTAG AAAACTATCT TGAATGGTTT 
AGATTCAGTG GTCGTATCCT GGGCCTAGCT CTGATCCATC AGTACCTCCT GGACGCTTTC 
TTCACAAGAC CCTTCTATAA GGGACTCCTG AAGCTGCCAT GTGATCTGAG TGACCTAGAA 
TATTTGGATG AGGAATTCCA CCAGAGTTTA CAGTGGATGA AGGACAACAA CATCACAGAC 
ATTCTAGATC TCACCTTCAC TGTTAATGAA GAAGTTTTTG GACAGGTAAC AGAAAGAGAG 
TTGAAGTCTG GAGGCGCCAA CACGCAGGTG ACTGAGAAAA ACAAGAAGGA ATACATAGAG 
AGGATGGTGA AGTGGCGTGT GGAGCGTGGT GTGGTACAGC AGACGGAGGC ATTGCTCAGG 
GGTTTCTATG AGGTTGTAGA CTCAAGGCTC GTCTCAGTGT TTGATGCGAG GGAACTGGAG 
CTGGTGATCG CTGGAACTGC TGAAATCGAC CTGAATGACT GGAGGAATAA CACAGAGTAC 
CGGGGAGGTT ATCACGATGG CCACCTTGTG ATCCGCTGGT TCTGGGCTGC AGTGGAACGC 
TTCAATAATG AGCAGAGATT GCGATTACTT CAGTTTGTGA CAGGAACATC AAGTGTGCCC 
TATGAAGGCT TTGCAGCTCT GCGGGGAAGC AATGGGCTTC GACGTTTCTG CATAGAAAAG 
TGGGGGAAGA TTACATCTCT TCCCAGGGCA CATACGTGCT TCAACCGATT GGATCTCCCA 
CCATACCCTT CATATTCCAT GTTGTATGAA AAGCTGTTAA CAGCAGTAGA AGAAACCAGC 
ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516813.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516876.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.

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CloneID	OMu48478
Clone ID Related Accession (Same CDS sequence)	XM_006516813.4 , XM_006516813.3
Accession Version	XM_006516813.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAATAAGCCA ACTTCCTTCC 
TTGAGGCCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516876.1
CDS	427..5202
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516813.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516813.3

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTGAATGTCC TATACTCCAT AATTCCCAGC CAATAAGCCA ACTTCCTTCC 
TTGAGGCCTG AACATCATCA CTACCCAACA ATCGATGAGC CTCTTCCACC AAACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516814.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516877.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.

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CloneID	OMu48479
Clone ID Related Accession (Same CDS sequence)	XM_006516814.4 , XM_006516814.3
Accession Version	XM_006516814.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGGTGA ATGTCCTATA 
CTCCATAATT CCCAGCCAAT AAGCCAACTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GAATTGACAG CCATGGACGG 
GTCTTTTATG TGGACCATAT AAATCGCACA ACCACCTGGC AGCGACCATC GATGGCCCCT 
ACCCCCGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGGAC CATGGCAACA GAGAGAGCTG AAGAAGATTC TGGCAATCAA 
AACTCTGAGC AAATACCAGA CGGAGGAGGA GGAGGTGGTG GTGGGAGTGA CTCGGAAGCA 
GAATCATCCC AATCAAGCTT AGATCTGAGG AGAGAAGGGT CACTTTCTCC TGTCAACTCA 
CAGAAGGTCA CCTTGCTGCT GCAGTCTCCA GCGGTCAAGT TCATCACCAA TCCTGAGTTC 
TTCACTGTGT TGCATGCCAA TTATAGTGCC TACAGGGTTT TCACAAGCAG CACTTGCCTG 
AAGCATATGA TTTTAAAAGT CCGAAGAGAT GCTCGAAATT TTGAGCGCTA TCAGCACAAC 
CGGGACCTGG TGAATTTCAT CAACATGTTT GCAGATACTC GGCTGGAACT GCCCCGGGGT 
TGGGAGATCA AAACTGATCA TCAGGGAAAG TCTTTCTTCG TGGACCACAA CAGTCGAGCT 
ACTACTTTCA TAGATCCCCG AATCCCTCTT CAGAATGGCC GTCTTCCCAA TCATCTAACC 
CACCGACAGC ACCTCCAGAG GCTCCGGAGT TACAGTGCTG GGGAGGCCTC AGAAGTCTCT 
AGAAACAGAG GAGCATCTTT GCTGGCCAGA CCAGGACACA GCCTAATAGC TGCTATTCGA 
AGCCAACATC AACATGAATC CTTGCCACTG GCATACAATG ACAAGATTGT GGCATTTCTT 
CGCCAGCCAA ACATTTTTGA AATGCTACAA GAACGTCAGC CAAGCTTGGC AAGAAACCAC 
ACACTCAGGG AGAAAATCCA TTATATTCGG ACAGAGGGCA ATCATGGGCT TGATAAATTG 
TCCTGTGATG CAGATCTAGT CATTTTGCTG AGTCTCTTTG AAGAAGAGAT CATGTCCTAT 
GTTCCCCTGC AATCTGCCTT CCACCCTGGA TATAGCTTCT CTCCCCGCTG TTCACCCTGT 
TCCTCACCTC AGAACTCCCC AGGTTTACAG AGAGCCAGCG CAAGAGCCCC TTCACCATAC 
CGAAGGGATT TTGAAGCTAA GCTCCGCAAT TTCTACCGAA AACTGGAAGC CAAAGGATTT 
GGACAGGGTC CGGGTAAAAT TAAGCTCATC ATCCGTCGAG ACCACTTGTT GGAGGGGACC 
TTCAATCAAG TGATGGCATA CTCAAGGAAG GAGCTCCAGA GAAACAAGCT CTACATCACC 
TTTGTTGGAG AGGAGGGGCT GGACTACAGT GGCCCTTCTC GAGAGTTCTT CTTCCTGTTG 
TCTCAGGAGC TCTTCAACCC TTACTATGGA CTCTTTGAGT ATTCTGCAAA TGACACATAC 
ACAGTACAGA TCAGCCCCAT GTCAGCATTT GTAGAAAACT ATCTTGAATG GTTTAGATTC 
AGTGGTCGTA TCCTGGGCCT AGCTCTGATC CATCAGTACC TCCTGGACGC TTTCTTCACA 
AGACCCTTCT ATAAGGGACT CCTGAAGCTG CCATGTGATC TGAGTGACCT AGAATATTTG 
GATGAGGAAT TCCACCAGAG TTTACAGTGG ATGAAGGACA ACAACATCAC AGACATTCTA 
GATCTCACCT TCACTGTTAA TGAAGAAGTT TTTGGACAGG TAACAGAAAG AGAGTTGAAG 
TCTGGAGGCG CCAACACGCA GGTGACTGAG AAAAACAAGA AGGAATACAT AGAGAGGATG 
GTGAAGTGGC GTGTGGAGCG TGGTGTGGTA CAGCAGACGG AGGCATTGCT CAGGGGTTTC 
TATGAGGTTG TAGACTCAAG GCTCGTCTCA GTGTTTGATG CGAGGGAACT GGAGCTGGTG 
ATCGCTGGAA CTGCTGAAAT CGACCTGAAT GACTGGAGGA ATAACACAGA GTACCGGGGA 
GGTTATCACG ATGGCCACCT TGTGATCCGC TGGTTCTGGG CTGCAGTGGA ACGCTTCAAT 
AATGAGCAGA GATTGCGATT ACTTCAGTTT GTGACAGGAA CATCAAGTGT GCCCTATGAA 
GGCTTTGCAG CTCTGCGGGG AAGCAATGGG CTTCGACGTT TCTGCATAGA AAAGTGGGGG 
AAGATTACAT CTCTTCCCAG GGCACATACG TGCTTCAACC GATTGGATCT CCCACCATAC 
CCTTCATATT CCATGTTGTA TGAAAAGCTG TTAACAGCAG TAGAAGAAAC CAGCACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516877.1
CDS	476..5227
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516814.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516814.3

ORF Insert Sequence:

ATGGCGTCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAACC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGCTATC 
ACCATCCCTC GCTCCACCAG TGACACTGAC CTGGTCACTT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCGATAGGG CACTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGATTA TAAGAACCGT GGAGTCAATG GTTCTCACAG AGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGTGG GGCCCTGCGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCAGCCC CTATTTTTAA AGGTATTGGC TCTGAAGAGA CTGCACAAAG TCAAGGAAGC 
CGGAGGCTGA TCAGCTTCTC CCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CCGACCCTTA TCTGAAGATT TCCATTCAAC CTGGGAAACA TAGCATCTTC 
CCAGCCCTCC CTCATCATGG ACAGGAAAGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACCGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCTG TTCAGAGGCT GCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCCGGCTTCC AACTGACCAT GTGAGTGGGC AGCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCATGC AGATGATGAA GAGATCTCTC TGAGTGCTGA GCCTGAGTCG 
TCAGCAGAAA CGCAGGACAG CATCATGAAT AGTATGGTGG GAAACAGCAA TGGAGAGCCT 
TCTGGTGATG CTACAGAATT CTGCAAGGAT GCGAAGCCAG AGTCACCAAG TGAAGGGAAT 
GGAGTAAACA GCTCAGAAAA CCAAAACCAG GAGCATGCCG GGCCAGTTGA GGAAGCGGCA 
GGAGCTATGG AGGCCAGAGA TGGTAGCAAT GTCTCTGAAG CACCCGAAGA GCCCGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACCCTGA GTGCAGAAGA AGTGGCCGAG 
GGACTTCCCC TGGATGAGGA TTCGCCATCC TCGCTGTTGC CAGAAGAGAA TACAGCTCTA 
GGCAGCAAGG TAGAAGAGGA AACAGTACCA GAAAATGGAG CCAGAGAGGA AGAAATGCAG 
AAGGGGAAAG ATGAAGAAGA GGAAGAAGAG GACGTTTCTA CCCTGGAGCA AGGAGAGCCT 
GGACTGGAGC TGAGGGTCTC CGTGAGAAAA AAAAGCAGAC CATGTTCCCT ACCTGTATCA 
GAGCTTGAGA CAGTGATTGC ATCTGCCTGT GGAGATGCTG AGACCCCGAG AACCCATTAT 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGAGAGGAAG CACCACGGAG 
GAGGAAGATG GCTTGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAGCGATGGG 
GCTACTCTGT GCATGGCACC CTCTGACTGC TCTGGGGGAC ATTTCTCCAG TTTATCCAAA 
GGCATTGGTG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGCAGCCCG 
TCTTGCTATA GCACATCTTG CTACAGCAGC TCTTGTTACA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGTAACAA TCGGTTCGCC AGCCACACAC GCTTCTCCTC TGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAAGA GGAAAACAGT 
GCATTCGAGT CGGTACCCGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGCC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAAGTACT 
GAAGGTCTGG AGTCCCCCAT GGCAGGTCCT AGCAATAGGA GAGAAGGTGA ATGTCCTATA 
CTCCATAATT CCCAGCCAAT AAGCCAACTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAGGCTC GAATTGACAG CCATGGACGG 
GTCTTTTATG TGGACCATAT AAATCGCACA ACCACCTGGC AGCGACCATC GATGGCCCCT 
ACCCCCGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGGAC CATGGCAACA GAGAGAGCTG AAGAAGATTC TGGCAATCAA 
AACTCTGAGC AAATACCAGA CGGAGGAGGA GGAGGTGGTG GTGGGAGTGA CTCGGAAGCA 
GAATCATCCC AATCAAGCTT AGATCTGAGG AGAGAAGGGT CACTTTCTCC TGTCAACTCA 
CAGAAGGTCA CCTTGCTGCT GCAGTCTCCA GCGGTCAAGT TCATCACCAA TCCTGAGTTC 
TTCACTGTGT TGCATGCCAA TTATAGTGCC TACAGGGTTT TCACAAGCAG CACTTGCCTG 
AAGCATATGA TTTTAAAAGT CCGAAGAGAT GCTCGAAATT TTGAGCGCTA TCAGCACAAC 
CGGGACCTGG TGAATTTCAT CAACATGTTT GCAGATACTC GGCTGGAACT GCCCCGGGGT 
TGGGAGATCA AAACTGATCA TCAGGGAAAG TCTTTCTTCG TGGACCACAA CAGTCGAGCT 
ACTACTTTCA TAGATCCCCG AATCCCTCTT CAGAATGGCC GTCTTCCCAA TCATCTAACC 
CACCGACAGC ACCTCCAGAG GCTCCGGAGT TACAGTGCTG GGGAGGCCTC AGAAGTCTCT 
AGAAACAGAG GAGCATCTTT GCTGGCCAGA CCAGGACACA GCCTAATAGC TGCTATTCGA 
AGCCAACATC AACATGAATC CTTGCCACTG GCATACAATG ACAAGATTGT GGCATTTCTT 
CGCCAGCCAA ACATTTTTGA AATGCTACAA GAACGTCAGC CAAGCTTGGC AAGAAACCAC 
ACACTCAGGG AGAAAATCCA TTATATTCGG ACAGAGGGCA ATCATGGGCT TGATAAATTG 
TCCTGTGATG CAGATCTAGT CATTTTGCTG AGTCTCTTTG AAGAAGAGAT CATGTCCTAT 
GTTCCCCTGC AATCTGCCTT CCACCCTGGA TATAGCTTCT CTCCCCGCTG TTCACCCTGT 
TCCTCACCTC AGAACTCCCC AGGTTTACAG AGAGCCAGCG CAAGAGCCCC TTCACCATAC 
CGAAGGGATT TTGAAGCTAA GCTCCGCAAT TTCTACCGAA AACTGGAAGC CAAAGGATTT 
GGACAGGGTC CGGGTAAAAT TAAGCTCATC ATCCGTCGAG ACCACTTGTT GGAGGGGACC 
TTCAATCAAG TGATGGCATA CTCAAGGAAG GAGCTCCAGA GAAACAAGCT CTACATCACC 
TTTGTTGGAG AGGAGGGGCT GGACTACAGT GGCCCTTCTC GAGAGTTCTT CTTCCTGTTG 
TCTCAGGAGC TCTTCAACCC TTACTATGGA CTCTTTGAGT ATTCTGCAAA TGACACATAC 
ACAGTACAGA TCAGCCCCAT GTCAGCATTT GTAGAAAACT ATCTTGAATG GTTTAGATTC 
AGTGGTCGTA TCCTGGGCCT AGCTCTGATC CATCAGTACC TCCTGGACGC TTTCTTCACA 
AGACCCTTCT ATAAGGGACT CCTGAAGCTG CCATGTGATC TGAGTGACCT AGAATATTTG 
GATGAGGAAT TCCACCAGAG TTTACAGTGG ATGAAGGACA ACAACATCAC AGACATTCTA 
GATCTCACCT TCACTGTTAA TGAAGAAGTT TTTGGACAGG TAACAGAAAG AGAGTTGAAG 
TCTGGAGGCG CCAACACGCA GGTGACTGAG AAAAACAAGA AGGAATACAT AGAGAGGATG 
GTGAAGTGGC GTGTGGAGCG TGGTGTGGTA CAGCAGACGG AGGCATTGCT CAGGGGTTTC 
TATGAGGTTG TAGACTCAAG GCTCGTCTCA GTGTTTGATG CGAGGGAACT GGAGCTGGTG 
ATCGCTGGAA CTGCTGAAAT CGACCTGAAT GACTGGAGGA ATAACACAGA GTACCGGGGA 
GGTTATCACG ATGGCCACCT TGTGATCCGC TGGTTCTGGG CTGCAGTGGA ACGCTTCAAT 
AATGAGCAGA GATTGCGATT ACTTCAGTTT GTGACAGGAA CATCAAGTGT GCCCTATGAA 
GGCTTTGCAG CTCTGCGGGG AAGCAATGGG CTTCGACGTT TCTGCATAGA AAAGTGGGGG 
AAGATTACAT CTCTTCCCAG GGCACATACG TGCTTCAACC GATTGGATCT CCCACCATAC 
CCTTCATATT CCATGTTGTA TGAAAAGCTG TTAACAGCAG TAGAAGAAAC CAGCACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516815.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516878.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.

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CloneID	OMu48480
Clone ID Related Accession (Same CDS sequence)	XM_006516815.4 , XM_006516815.3
Accession Version	XM_006516815.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516878.1
CDS	427..5142
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516815.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516815.3

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG GTCTGGAGTC CCCCATGGCA GGTCCTAGCA ATAGGAGAGA AGACTGGGAG 
GCTCGAATTG ACAGCCATGG ACGGGTCTTT TATGTGGACC ATATAAATCG CACAACCACC 
TGGCAGCGAC CATCGATGGC CCCTACCCCC GATGGAATGA TCAGATCCGG GTCTGTCCAC 
CAGATGGAGC AGCTTAACAG AAGGTATCAA AACATCCAGA GGACCATGGC AACAGAGAGA 
GCTGAAGAAG ATTCTGGCAA TCAAAACTCT GAGCAAATAC CAGACGGAGG AGGAGGAGGT 
GGTGGTGGGA GTGACTCGGA AGCAGAATCA TCCCAATCAA GCTTAGATCT GAGGAGAGAA 
GGGTCACTTT CTCCTGTCAA CTCACAGAAG GTCACCTTGC TGCTGCAGTC TCCAGCGGTC 
AAGTTCATCA CCAATCCTGA GTTCTTCACT GTGTTGCATG CCAATTATAG TGCCTACAGG 
GTTTTCACAA GCAGCACTTG CCTGAAGCAT ATGATTTTAA AAGTCCGAAG AGATGCTCGA 
AATTTTGAGC GCTATCAGCA CAACCGGGAC CTGGTGAATT TCATCAACAT GTTTGCAGAT 
ACTCGGCTGG AACTGCCCCG GGGTTGGGAG ATCAAAACTG ATCATCAGGG AAAGTCTTTC 
TTCGTGGACC ACAACAGTCG AGCTACTACT TTCATAGATC CCCGAATCCC TCTTCAGAAT 
GGCCGTCTTC CCAATCATCT AACCCACCGA CAGCACCTCC AGAGGCTCCG GAGTTACAGT 
GCTGGGGAGG CCTCAGAAGT CTCTAGAAAC AGAGGAGCAT CTTTGCTGGC CAGACCAGGA 
CACAGCCTAA TAGCTGCTAT TCGAAGCCAA CATCAACATG AATCCTTGCC ACTGGCATAC 
AATGACAAGA TTGTGGCATT TCTTCGCCAG CCAAACATTT TTGAAATGCT ACAAGAACGT 
CAGCCAAGCT TGGCAAGAAA CCACACACTC AGGGAGAAAA TCCATTATAT TCGGACAGAG 
GGCAATCATG GGCTTGATAA ATTGTCCTGT GATGCAGATC TAGTCATTTT GCTGAGTCTC 
TTTGAAGAAG AGATCATGTC CTATGTTCCC CTGCAATCTG CCTTCCACCC TGGATATAGC 
TTCTCTCCCC GCTGTTCACC CTGTTCCTCA CCTCAGAACT CCCCAGGTTT ACAGAGAGCC 
AGCGCAAGAG CCCCTTCACC ATACCGAAGG GATTTTGAAG CTAAGCTCCG CAATTTCTAC 
CGAAAACTGG AAGCCAAAGG ATTTGGACAG GGTCCGGGTA AAATTAAGCT CATCATCCGT 
CGAGACCACT TGTTGGAGGG GACCTTCAAT CAAGTGATGG CATACTCAAG GAAGGAGCTC 
CAGAGAAACA AGCTCTACAT CACCTTTGTT GGAGAGGAGG GGCTGGACTA CAGTGGCCCT 
TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG GAGCTCTTCA ACCCTTACTA TGGACTCTTT 
GAGTATTCTG CAAATGACAC ATACACAGTA CAGATCAGCC CCATGTCAGC ATTTGTAGAA 
AACTATCTTG AATGGTTTAG ATTCAGTGGT CGTATCCTGG GCCTAGCTCT GATCCATCAG 
TACCTCCTGG ACGCTTTCTT CACAAGACCC TTCTATAAGG GACTCCTGAA GCTGCCATGT 
GATCTGAGTG ACCTAGAATA TTTGGATGAG GAATTCCACC AGAGTTTACA GTGGATGAAG 
GACAACAACA TCACAGACAT TCTAGATCTC ACCTTCACTG TTAATGAAGA AGTTTTTGGA 
CAGGTAACAG AAAGAGAGTT GAAGTCTGGA GGCGCCAACA CGCAGGTGAC TGAGAAAAAC 
AAGAAGGAAT ACATAGAGAG GATGGTGAAG TGGCGTGTGG AGCGTGGTGT GGTACAGCAG 
ACGGAGGCAT TGCTCAGGGG TTTCTATGAG GTTGTAGACT CAAGGCTCGT CTCAGTGTTT 
GATGCGAGGG AACTGGAGCT GGTGATCGCT GGAACTGCTG AAATCGACCT GAATGACTGG 
AGGAATAACA CAGAGTACCG GGGAGGTTAT CACGATGGCC ACCTTGTGAT CCGCTGGTTC 
TGGGCTGCAG TGGAACGCTT CAATAATGAG CAGAGATTGC GATTACTTCA GTTTGTGACA 
GGAACATCAA GTGTGCCCTA TGAAGGCTTT GCAGCTCTGC GGGGAAGCAA TGGGCTTCGA 
CGTTTCTGCA TAGAAAAGTG GGGGAAGATT ACATCTCTTC CCAGGGCACA TACGTGCTTC 
AACCGATTGG ATCTCCCACC ATACCCTTCA TATTCCATGT TGTATGAAAA GCTGTTAACA 
GCAGTAGAAG AAACCAGCAC CTTTGGACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_006516816.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006516879.1 Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X9, mRNA.

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CloneID	OMu48481
Clone ID Related Accession (Same CDS sequence)	XM_006516816.4 , XM_006516816.3
Accession Version	XM_006516816.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG ACTGGGAGGC TCGAATTGAC AGCCATGGAC GGGTCTTTTA TGTGGACCAT 
ATAAATCGCA CAACCACCTG GCAGCGACCA TCGATGGCCC CTACCCCCGA TGGAATGATC 
AGATCCGGGT CTGTCCACCA GATGGAGCAG CTTAACAGAA GGTATCAAAA CATCCAGAGG 
ACCATGGCAA CAGAGAGAGC TGAAGAAGAT TCTGGCAATC AAAACTCTGA GCAAATACCA 
GACGGAGGAG GAGGAGGTGG TGGTGGGAGT GACTCGGAAG CAGAATCATC CCAATCAAGC 
TTAGATCTGA GGAGAGAAGG GTCACTTTCT CCTGTCAACT CACAGAAGGT CACCTTGCTG 
CTGCAGTCTC CAGCGGTCAA GTTCATCACC AATCCTGAGT TCTTCACTGT GTTGCATGCC 
AATTATAGTG CCTACAGGGT TTTCACAAGC AGCACTTGCC TGAAGCATAT GATTTTAAAA 
GTCCGAAGAG ATGCTCGAAA TTTTGAGCGC TATCAGCACA ACCGGGACCT GGTGAATTTC 
ATCAACATGT TTGCAGATAC TCGGCTGGAA CTGCCCCGGG GTTGGGAGAT CAAAACTGAT 
CATCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACTACTTT CATAGATCCC 
CGAATCCCTC TTCAGAATGG CCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGGCTCCGGA GTTACAGTGC TGGGGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCATCT 
TTGCTGGCCA GACCAGGACA CAGCCTAATA GCTGCTATTC GAAGCCAACA TCAACATGAA 
TCCTTGCCAC TGGCATACAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTAC AAGAACGTCA GCCAAGCTTG GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTATATTC GGACAGAGGG CAATCATGGG CTTGATAAAT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATCATGTCCT ATGTTCCCCT GCAATCTGCC 
TTCCACCCTG GATATAGCTT CTCTCCCCGC TGTTCACCCT GTTCCTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCACCAT ACCGAAGGGA TTTTGAAGCT 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGACAGGG TCCGGGTAAA 
ATTAAGCTCA TCATCCGTCG AGACCACTTG TTGGAGGGGA CCTTCAATCA AGTGATGGCA 
TACTCAAGGA AGGAGCTCCA GAGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGG 
CTGGACTACA GTGGCCCTTC TCGAGAGTTC TTCTTCCTGT TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTATTCTGCA AATGACACAT ACACAGTACA GATCAGCCCC 
ATGTCAGCAT TTGTAGAAAA CTATCTTGAA TGGTTTAGAT TCAGTGGTCG TATCCTGGGC 
CTAGCTCTGA TCCATCAGTA CCTCCTGGAC GCTTTCTTCA CAAGACCCTT CTATAAGGGA 
CTCCTGAAGC TGCCATGTGA TCTGAGTGAC CTAGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTACAGT GGATGAAGGA CAACAACATC ACAGACATTC TAGATCTCAC CTTCACTGTT 
AATGAAGAAG TTTTTGGACA GGTAACAGAA AGAGAGTTGA AGTCTGGAGG CGCCAACACG 
CAGGTGACTG AGAAAAACAA GAAGGAATAC ATAGAGAGGA TGGTGAAGTG GCGTGTGGAG 
CGTGGTGTGG TACAGCAGAC GGAGGCATTG CTCAGGGGTT TCTATGAGGT TGTAGACTCA 
AGGCTCGTCT CAGTGTTTGA TGCGAGGGAA CTGGAGCTGG TGATCGCTGG AACTGCTGAA 
ATCGACCTGA ATGACTGGAG GAATAACACA GAGTACCGGG GAGGTTATCA CGATGGCCAC 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAACGCTTCA ATAATGAGCA GAGATTGCGA 
TTACTTCAGT TTGTGACAGG AACATCAAGT GTGCCCTATG AAGGCTTTGC AGCTCTGCGG 
GGAAGCAATG GGCTTCGACG TTTCTGCATA GAAAAGTGGG GGAAGATTAC ATCTCTTCCC 
AGGGCACATA CGTGCTTCAA CCGATTGGAT CTCCCACCAT ACCCTTCATA TTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006516879.1
CDS	427..5100
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEDWEARIDSHGRVFYVDHINRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006516816.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006516816.3

ORF Insert Sequence:

ATGATCTGTG CCCAGTTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CGTCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAACCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GCTATCACCA TCCCTCGCTC CACCAGTGAC ACTGACCTGG TCACTTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCG ATAGGGCACT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCTGGA GACTGGATTG GCATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGATTATAAG AACCGTGGAG TCAATGGTTC TCACAGAGGC 
CAGATCATCT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGTGGGGCC CTGCGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CAGCCCCTAT TTTTAAAGGT ATTGGCTCTG AAGAGACTGC ACAAAGTCAA 
GGAAGCCGGA GGCTGATCAG CTTCTCCCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCCGA CCCTTATCTG AAGATTTCCA TTCAACCTGG GAAACATAGC 
ATCTTCCCAG CCCTCCCTCA TCATGGACAG GAAAGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC CGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCTGTTCA GAGGCTGCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCCG GCTTCCAACT GACCATGTGA GTGGGCAGCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCATGCAGAT GATGAAGAGA TCTCTCTGAG TGCTGAGCCT 
GAGTCGTCAG CAGAAACGCA GGACAGCATC ATGAATAGTA TGGTGGGAAA CAGCAATGGA 
GAGCCTTCTG GTGATGCTAC AGAATTCTGC AAGGATGCGA AGCCAGAGTC ACCAAGTGAA 
GGGAATGGAG TAAACAGCTC AGAAAACCAA AACCAGGAGC ATGCCGGGCC AGTTGAGGAA 
GCGGCAGGAG CTATGGAGGC CAGAGATGGT AGCAATGTCT CTGAAGCACC CGAAGAGCCC 
GGAGAGCTTC AGGACCCAGA GCAACATGAC ACCCAGCCCA CCCTGAGTGC AGAAGAAGTG 
GCCGAGGGAC TTCCCCTGGA TGAGGATTCG CCATCCTCGC TGTTGCCAGA AGAGAATACA 
GCTCTAGGCA GCAAGGTAGA AGAGGAAACA GTACCAGAAA ATGGAGCCAG AGAGGAAGAA 
ATGCAGAAGG GGAAAGATGA AGAAGAGGAA GAAGAGGACG TTTCTACCCT GGAGCAAGGA 
GAGCCTGGAC TGGAGCTGAG GGTCTCCGTG AGAAAAAAAA GCAGACCATG TTCCCTACCT 
GTATCAGAGC TTGAGACAGT GATTGCATCT GCCTGTGGAG ATGCTGAGAC CCCGAGAACC 
CATTATATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGAG AGGAAGCACC 
ACGGAGGAGG AAGATGGCTT GGAGGAGGAA TCCACCCTCA AGGAGTCCTC TGAAAAGGAT 
GGGCTCAGCG AGGTGGACAC AATAGCTGCT GACCCACAGT CCATGGAAGA TGGCGAGAGC 
GATGGGGCTA CTCTGTGCAT GGCACCCTCT GACTGCTCTG GGGGACATTT CTCCAGTTTA 
TCCAAAGGCA TTGGTGCTGG CCAAGATGGC GAAGCTCACC CCAGCACTGG GAGTGAGAGT 
GACTCCAGCC CCCAGCAGGG AGCAGACCAC AGTTGCGAGG GCTGTGATGC CTCATGCTGC 
AGCCCGTCTT GCTATAGCAC ATCTTGCTAC AGCAGCTCTT GTTACAGCAG CTCCTGTTAC 
AGCAGCTCCT GCTACAATGG TAACAATCGG TTCGCCAGCC ACACACGCTT CTCCTCTGTG 
GACAGCGCCA AGATCTCTGA GAGCACAGTC TTTTCTTCAC AAGAGGATGA GGAAGAGGAA 
AACAGTGCAT TCGAGTCGGT ACCCGACTCG GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT 
ACAAATGGGG CTGGCCCTTG GCAGGATGAA CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA 
AGTACTGAAG ACTGGGAGGC TCGAATTGAC AGCCATGGAC GGGTCTTTTA TGTGGACCAT 
ATAAATCGCA CAACCACCTG GCAGCGACCA TCGATGGCCC CTACCCCCGA TGGAATGATC 
AGATCCGGGT CTGTCCACCA GATGGAGCAG CTTAACAGAA GGTATCAAAA CATCCAGAGG 
ACCATGGCAA CAGAGAGAGC TGAAGAAGAT TCTGGCAATC AAAACTCTGA GCAAATACCA 
GACGGAGGAG GAGGAGGTGG TGGTGGGAGT GACTCGGAAG CAGAATCATC CCAATCAAGC 
TTAGATCTGA GGAGAGAAGG GTCACTTTCT CCTGTCAACT CACAGAAGGT CACCTTGCTG 
CTGCAGTCTC CAGCGGTCAA GTTCATCACC AATCCTGAGT TCTTCACTGT GTTGCATGCC 
AATTATAGTG CCTACAGGGT TTTCACAAGC AGCACTTGCC TGAAGCATAT GATTTTAAAA 
GTCCGAAGAG ATGCTCGAAA TTTTGAGCGC TATCAGCACA ACCGGGACCT GGTGAATTTC 
ATCAACATGT TTGCAGATAC TCGGCTGGAA CTGCCCCGGG GTTGGGAGAT CAAAACTGAT 
CATCAGGGAA AGTCTTTCTT CGTGGACCAC AACAGTCGAG CTACTACTTT CATAGATCCC 
CGAATCCCTC TTCAGAATGG CCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGGCTCCGGA GTTACAGTGC TGGGGAGGCC TCAGAAGTCT CTAGAAACAG AGGAGCATCT 
TTGCTGGCCA GACCAGGACA CAGCCTAATA GCTGCTATTC GAAGCCAACA TCAACATGAA 
TCCTTGCCAC TGGCATACAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTAC AAGAACGTCA GCCAAGCTTG GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTATATTC GGACAGAGGG CAATCATGGG CTTGATAAAT TGTCCTGTGA TGCAGATCTA 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATCATGTCCT ATGTTCCCCT GCAATCTGCC 
TTCCACCCTG GATATAGCTT CTCTCCCCGC TGTTCACCCT GTTCCTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCACCAT ACCGAAGGGA TTTTGAAGCT 
AAGCTCCGCA ATTTCTACCG AAAACTGGAA GCCAAAGGAT TTGGACAGGG TCCGGGTAAA 
ATTAAGCTCA TCATCCGTCG AGACCACTTG TTGGAGGGGA CCTTCAATCA AGTGATGGCA 
TACTCAAGGA AGGAGCTCCA GAGAAACAAG CTCTACATCA CCTTTGTTGG AGAGGAGGGG 
CTGGACTACA GTGGCCCTTC TCGAGAGTTC TTCTTCCTGT TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTATTCTGCA AATGACACAT ACACAGTACA GATCAGCCCC 
ATGTCAGCAT TTGTAGAAAA CTATCTTGAA TGGTTTAGAT TCAGTGGTCG TATCCTGGGC 
CTAGCTCTGA TCCATCAGTA CCTCCTGGAC GCTTTCTTCA CAAGACCCTT CTATAAGGGA 
CTCCTGAAGC TGCCATGTGA TCTGAGTGAC CTAGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTACAGT GGATGAAGGA CAACAACATC ACAGACATTC TAGATCTCAC CTTCACTGTT 
AATGAAGAAG TTTTTGGACA GGTAACAGAA AGAGAGTTGA AGTCTGGAGG CGCCAACACG 
CAGGTGACTG AGAAAAACAA GAAGGAATAC ATAGAGAGGA TGGTGAAGTG GCGTGTGGAG 
CGTGGTGTGG TACAGCAGAC GGAGGCATTG CTCAGGGGTT TCTATGAGGT TGTAGACTCA 
AGGCTCGTCT CAGTGTTTGA TGCGAGGGAA CTGGAGCTGG TGATCGCTGG AACTGCTGAA 
ATCGACCTGA ATGACTGGAG GAATAACACA GAGTACCGGG GAGGTTATCA CGATGGCCAC 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAACGCTTCA ATAATGAGCA GAGATTGCGA 
TTACTTCAGT TTGTGACAGG AACATCAAGT GTGCCCTATG AAGGCTTTGC AGCTCTGCGG 
GGAAGCAATG GGCTTCGACG TTTCTGCATA GAAAAGTGGG GGAAGATTAC ATCTCTTCCC 
AGGGCACATA CGTGCTTCAA CCGATTGGAT CTCCCACCAT ACCCTTCATA TTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAAGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Hecw1
Entrez Gene ID	94253
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms	9330116H24Rik,AV273951,E130207I19Rik,Nedl1
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HECW1 HECT type E3 ubiquitin ligase HECT, C2 and WW domain-containing protein 1 HECT-type E3 ubiquitin transferase HECW1 NEDD4-like E3 ubiquitin-protein ligase 1 mNEDL1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A1\|13 5.29 cM

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Hecw1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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REQUEST A QUOTE

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